LOCUS       NZ_KZ195574            10600 bp    DNA     linear   CON 06-SEP-2024
DEFINITION  Streptomyces sp. CS113 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ195574
VERSION     NZ_KZ195574.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS113
  ORGANISM  Streptomyces sp. CS113
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8695358)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8695358)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8695358)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 78.17x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188365.1-RS_2024_09_06
            Annotation Date                   :: 09/06/2024 02:31:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,790
            CDSs (total)                      :: 7,719
            Genes (coding)                    :: 7,614
            CDSs (with protein)               :: 7,614
            Genes (RNA)                       :: 71
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 35 of 105
            Pseudo Genes (incomplete)         :: 81 of 105
            Pseudo Genes (internal stop)      :: 4 of 105
            Pseudo Genes (multiple problems)  :: 15 of 105
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:56:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5614826
            Orig. end                         :: 5625426
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195574.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
FEATURES             Location/Qualifiers
     region          1..10600
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="24"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10600
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10600
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5600](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5600
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(722..2203)
                     /locus_tag="B9W62_RS25120"
                     /old_locus_tag="B9W62_25145"
     CDS             complement(722..2203)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028527.1"
                     /locus_tag="B9W62_RS25120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_25145"
                     /product="glycosyltransferase"
                     /protein_id="WP_373433703.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 3.2e-33,
                     bitscore: 113.6, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLSPTGSRPRVLHVTQPADGGVARVVAELTRAQLAAGLRVVVAC
                     PAGGLAGRLRALGADVRHWPATRSPGPALVAEVRRLCRVIEDVRPDLVHAHSAKAGLAG
                     RLAVRGRIPTVFQPHAWSFEAVGGSAARLALGWERWSGRWTARTVCVSEAERLRGVRAG
                     LRGPWSVVPNGVDLARFPADVPGYGHDDPAGPRLRHGVAADTPLVVCVGRLCRQKGQDV
                     LLGAWESVRARVPDARLVLVGDGPDRARLAARAPASVLFTGAVDDAAAWYRAADLVVLP
                     SRWEGMALAPLEAMACGRPVVVTDVDGAREGLPPALVPHCLVPPGDPAALADATAALLL
                     DAPLRASLGRRGRAHVRSAHDVRHTAAAIAALYRDLLDGVPDDDLLDGDPLDGFPDDGL
                     PDGEPLDGPPDGGLPDGDLHDDLPDGGLLDGFPDDVRPAATATPAAAAVARGADAVGGV
                     RGRPPTAPRLLPGEPPAAAGRSAVVPIECRESTHP"
     gene            2490..2729
                     /gene="chpG"
                     /locus_tag="B9W62_RS25125"
                     /old_locus_tag="B9W62_25150"
     CDS             2490..2729
                     /codon_start=1
                     /gene="chpG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449696.1"
                     /locus_tag="B9W62_RS25125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_25150"
                     /product="chaplin ChpG"
                     /protein_id="WP_030179666.1"
                     /transl_table=11
                     /translation="MSRIAKGLALTSVAAAAVAGTAGVAAADSGAQAAAAHSPGVLSGN
                     VVQVPVHIPVNVCGNTIDIIGLLNPAFGNECEND"
     gene            3005..3754
                     /locus_tag="B9W62_RS25130"
                     /old_locus_tag="B9W62_25155"
     CDS             3005..3754
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976096.1"
                     /locus_tag="B9W62_RS25130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_25155"
                     /product="hypothetical protein"
                     /protein_id="WP_087809272.1"
                     /transl_table=11
                     /translation="MRLPARRIATSALCAGLLIGLSGPAVMAADGDSVRERTHAASHAP
                     LPDAEELQSQVGSLAGLGGVLTPVTDMLSAVLKADNGQLSATDADKLSAAVKDALAKAD
                     AADADADDVATTPGTTTPGTTTPAQPPAVTAPEAGTDTPVTLPAPVTAQSDDGTAAATD
                     LSAAAEAALQKQIDALVKATTSGTAEQVSPAVKDLMTGIVNVVAATMVGNGLPAADLAG
                     LPGVPTAPEATTPEAATPEVAAPADPS"
     gene            3942..4244
                     /locus_tag="B9W62_RS25135"
                     /old_locus_tag="B9W62_25160"
     CDS             3942..4244
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445910.1"
                     /locus_tag="B9W62_RS25135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_25160"
                     /product="hypothetical protein"
                     /protein_id="WP_087809273.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSLIAAGSAAPAFAYDASDAAALTPTSLNGAVNA
                     LTAEPIDIMPIKHQSEGRSDALDTENKDSVLHTVKGATTKLNSANGLLGGLPLGG"
     gene            4365..4880
                     /locus_tag="B9W62_RS25140"
                     /old_locus_tag="B9W62_25165"
     CDS             4365..4880
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="B9W62_RS25140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_25165"
                     /product="hypothetical protein"
                     /protein_id="WP_087809274.1"
                     /transl_table=11
                     /translation="MKPTTRRTLAAVVTGVAAAVGVAATPAAAARAVPVPVPLGGAETA
                     LGMELPEVAGELPVPTAGAPEGPRYVEGRLLPERTLPQLPVRGGLPGVDVRQPLPHILG
                     DDFDHAGVDAPAAELRTLAPGLSLDAPLTAPNPEALGLPAPKLPEVGVLAPVVQTVPVA
                     DLGVGPGR"
     gene            5001..5600
                     /locus_tag="B9W62_RS25145"
                     /old_locus_tag="B9W62_25170"
     CDS             5001..5600
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028525.1"
                     /locus_tag="B9W62_RS25145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_25170"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_087809275.1"
                     /sec_met_domain="melC (E-value: 2e-49, bitscore: 166.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGNTGATVNGATERGTERAYAAEDTASGGPSSGTRRQVMRGLLA
                     PALAVGLAPLIAASRPSGAAEAAEAATTGPRPARPGHPGPHAVESAQSAESADGAESTA
                     FDETYRGRRIRGVRSAAGRAVGAGTWQVTVDGRPLHLMRRADGSWLSMVDHYRSYPTPL
                     AAARGAVDELGPGEHLRDTPSIGHDQHSGGRHGVHA"
     gene            5584..6450
                     /locus_tag="B9W62_RS25150"
                     /old_locus_tag="B9W62_25175"
     CDS             5584..6450
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="B9W62_RS25150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_25175"
                     /product="tyrosinase family protein"
                     /protein_id="WP_087809276.1"
                     /transl_table=11
                     /translation="MAYTRKDVSTLTRTEKRRFVNALLEIKRRGEYDEFVRTHIEYYVS
                     DGEDGLRTAHMAPSFLPWHRRFLLDLEEALRRVDDAVTVPYWDWTKDRGAKSTPWTADL
                     LGGTGRRSDHRVTTGPFAHAGGNWTLKVNVTDTEYLTRDLGRAGDPLGLPSKSDLEWAL
                     DDPAYDTSPYDSTVRKGFRNKLEGWGTGRGEVSWRNHNRVHRWVGGAMVGGASVNDPVF
                     WLHHAFVDLQWSRWQARHRGARYLPAEPPGRGSAQRGRIVARHERLPPWDVTPDELEDV
                     GRIYRYA"
     gene            complement(6608..6883)
                     /gene="chpF"
                     /locus_tag="B9W62_RS25155"
                     /old_locus_tag="B9W62_25180"
     CDS             complement(6608..6883)
                     /codon_start=1
                     /gene="chpF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028524.1"
                     /locus_tag="B9W62_RS25155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_25180"
                     /product="chaplin ChpF"
                     /protein_id="WP_087809277.1"
                     /transl_table=11
                     /translation="MSRIAKAAGVALGAGAVVLSGTGMAMADAGAAGAAVGSPGVLSGN
                     VVQIPVHVPVNLCGNTIDVIGLLNPAFGNACENGDDDGDKSGGYGG"
     gene            7062..7526
                     /locus_tag="B9W62_RS40840"
                     /old_locus_tag="B9W62_25185"
     CDS             7062..7526
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007499745.1"
                     /locus_tag="B9W62_RS40840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_25185"
                     /product="DUF2087 domain-containing protein"
                     /protein_id="WP_256976573.1"
                     /transl_table=11
                     /translation="MPNDRSDSPVEDPESSQPPQQSQLSQQPQLSQSSQSSQPLQPLPP
                     LQPSPDTGAAGGDARDGMIVRTFVRDGRLVRLPARWTRKKVVLRHIAEQTFVPGVEYPE
                     RTVDEKLRAWCEDGEVDHVTLRRHLVDFGHLRRSDGIYARPADSARTGSA"
     gene            complement(7533..8522)
                     /locus_tag="B9W62_RS25170"
                     /old_locus_tag="B9W62_25190"
     CDS             complement(7533..8522)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028521.1"
                     /locus_tag="B9W62_RS25170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_25190"
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_087812089.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 3.6e-51, bitscore:
                     171.8, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDFQQVATEIADWSPVADDVDVVSYATHFPDEDALAA
                     ALADADIVVTLRERVPFPGSLLARLPRLKLLVASGMRNSVIDYAAAEAHGVTVCGTASS
                     STPPVELTWALLLGLARGIVQEGNGLREGGPWQQTVGADLHGRRLGLLGLGKIGGRVAQ
                     VGLAFGMRVSAWSQNLTRERTDEVGAELARSKEELLSTADFVSVHLALGERTRGLLGPA
                     ELALLKPTAYLVNTSRAAIVDQEALLAALHEGRIAGAGVDVFDTEPLPAGHPMRTAPRL
                     LATPHLGYVSRANYATYYGQAVEAIAAYLAGSPVRRLPVPAPAAPNPA"
     misc_feature    complement(8658..8673)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.49"
                     /tool="antismash"
     tRNA            complement(8686..8758)
                     /anticodon=(pos:complement(5623549..5623551),aa:Arg,
                     seq:tct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="B9W62_RS25175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="B9W62_25195"
                     /product="tRNA-Arg"
     gene            complement(8686..8758)
                     /locus_tag="B9W62_RS25175"
                     /old_locus_tag="B9W62_25195"
     misc_feature    9031..9046
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.68"
                     /tool="antismash"
     misc_feature    9033..9047
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.51"
                     /tool="antismash"
     misc_feature    complement(9034..9049)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.93"
                     /tool="antismash"
     misc_feature    complement(9036..9049)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.26"
                     /tool="antismash"
     misc_feature    complement(9058..9072)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.23"
                     /tool="antismash"
     misc_feature    9061..9077
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.29"
                     /tool="antismash"
ORIGIN
        1 gccgcctgcc ccacccgtac ggaggggtcc acggccagca cgtcccgtac gccgttctgc
       61 acgaccgccc gccgcacctc ctcgccggtg gacagcacgg gcaggcccgc ggcgccgccc
      121 gcgtcgccgg tgaccacgcc gacggggcgc accccgcagc gcgggtggcg cagcagcgcc
      181 gcggccacgc gctgcgcggt cgcggccggt ccgacgacga gggcggcgcg ggggcggttc
      241 agcagcgccg tacggtgcgt gcggtgcacg gcgccccgtc cggcgcagcc cgtcaccgcg
      301 tgcagcagga aaccggcgca cagggtgcgt acggacagcg cgtgcggcgg agcgtacgcc
      361 gcgacgagcg ccgccagcgc cagccagacc accgcgaccc gggcgcacag ggcgggcagg
      421 tcgtccagcg cgccggaggt ccgccgggcc gggcgcggcc gggggcgcag cagcagcgag
      481 gcggccacca gcagggccgc gagcagcggt tggtgcgggg tgccggtgag gaccagcgag
      541 cccggcaggg ccgccgcgcc gtcggtgacg agcagcggca gcggggagtc cgggcgcggg
      601 gcggggcgcc gggcgggcag ccgggggccc ggggcgccgt cgcgccgcgg cagcaccgag
      661 acgggggagg agccgaggtc gcgcgagggg ccggcggggg aggggacggt gctttccgcg
      721 gtcacgggtg ggtggactcc ctgcactcga tgggcacgac ggcggaccgc cccgcggccg
      781 cgggcggctc gccggggagg aggcgcggtg cggtgggcgg tctgccgcgg acgccgccga
      841 ccgcgtcggc gccgcgcgcc acggccgcgg ccgcgggcgt ggccgtggcc gcgggccgta
      901 cgtcgtcggg gaagccatcg aggaggccgc cgtcgggaag gtcgtcgtgc aggtcgccgt
      961 cgggaaggcc gccgtcggga gggccgtcga gcggctcgcc gtcgggaagt ccgtcgtcgg
     1021 ggaagccgtc gagcgggtcg ccgtcgagga gatcgtcgtc gggcacgccg tcgaggaggt
     1081 cgcggtagag ggccgcgatc gccgcggccg tgtgccgtac gtcgtgcgcg gaccggacgt
     1141 gcgcccgccc ccggcggccg agcgaggcgc gcagcggcgc gtcgagcagc agcgcggcgg
     1201 tggcgtcggc cagcgccgcg ggatccccgg gcggcaccaa gcagtgcggc accagcgcgg
     1261 gcggcaggcc ctcgcgggca ccgtccacgt cggtcaccac caccggccgc ccgcaggcca
     1321 tcgcctccag cggggccagc gccatgccct cccagcgcga cggcaggacg accaggtcgg
     1381 cggcccggta ccaggcggcg gcgtcgtcga cggcccccgt gaacagcacg gacgccgggg
     1441 cccgcgccgc gagccgggcg cggtccgggc cgtcaccgac caggaccagc cgggcgtcgg
     1501 gcacccgcgc gcgcaccgac tcccaggcgc ccagcagcac gtcctgcccc ttctgccggc
     1561 acagccgccc cacacagacc accagcgggg tgtccgcggc gacgccgtgc cgcaggcgcg
     1621 gaccggccgg gtcgtcgtgg ccgtacccgg gcacgtcggc ggggaagcgg gcgaggtcga
     1681 cgccgttcgg gacgacggac cagggcccgc gcagcccggc ccgcaccccc cgcagccgtt
     1741 ccgcctcgct cacgcacacc gtccgcgccg tccaccgccc cgaccaccgc tcccagccca
     1801 gcgcgaggcg tgcggcggac ccgccgaccg cctcgaacga ccaggcgtgc ggctggaaca
     1861 cggtggggat ccggccccgt acggcgagcc gtccggccag accggccttc gcactgtgcg
     1921 cgtgcaccag gtcgggccgt acgtcctcga tcacgcggca cagccgccgt acctccgcca
     1981 ccagcgccgg gcccggcgaa cgggtggccg gccagtgccg cacgtcggcg cccagcgccc
     2041 gcagccggcc ggcgaggccc ccggcgggac aggcgaccac gacccgcagt ccggcggcga
     2101 gctgagcccg ggtcagctcc gccaccaccc gggcgacccc gccgtccgcc ggttgggtga
     2161 cgtgcagaac ccgtggccgc gagccggtcg gtgacaggtg catgcgcgtt tcctcgctca
     2221 cggagattgc tcgggacggg cgggaacgcg cccggccgcg gggcgccggc cgcgacgcgg
     2281 catacgggtg cgtcgaatgg gtcgaatggt gcgggactct agctgctatc cgtactaata
     2341 cggtgaaacc gggtaagtgt gttggatttg tgaacgccgg tctttcgcga gatcacccct
     2401 ttggcggcac aactcgcgct cgcgccacgc gttgacactg cgccgggcag ccgcccggat
     2461 gtgtgtacaa ccccaaggag cacttctcca tgtcgcgtat cgcgaagggc ctggccctga
     2521 cctccgtcgc cgccgccgcg gtggcgggca ccgccggtgt cgccgccgcc gacagcggcg
     2581 cgcaggccgc cgccgcccac tccccgggcg tcctgtccgg caacgtggtg caggtcccgg
     2641 tccacatccc ggtcaacgtc tgcggtaaca ccatcgacat catcggtctg ctgaacccgg
     2701 cgttcggcaa cgagtgcgag aacgactgac gtcggctcac cactctccgt accggccgtc
     2761 cgtaccgcgg ttcctccgcg agcgggcggc cggttcgcgt tgccgcgcgg acggcggtgt
     2821 gctgccccga atggggggtc cgggcggcga cacggcgcac ggcccttgac agggtgtctc
     2881 accaggtaag acgcggccgg tgagaagggc gggcgggcgc acgggctcgc cgcctgcgtt
     2941 gcagagcgcc aagggcgctt ccacggtggg cacaacatcc gacgcaccac cgaaaggaac
     3001 ccccatgcgt ctgcccgcac ggcgaatcgc cacctccgct ctctgcgccg gcctgctgat
     3061 cggcctctcc ggtccggcgg tgatggcggc cgacggcgac tcggtccggg aacgcaccca
     3121 cgcggcgtcc cacgcacccc tccccgacgc ggaggaactg caaagccagg tcggcagcct
     3181 ggccggcctg ggcggagtgc tcacgcccgt caccgacatg ctcagcgccg tcctcaaggc
     3241 cgacaacggc cagctctccg ccaccgacgc ggacaagctc tccgccgccg tcaaggacgc
     3301 cctcgccaag gcggacgccg cggacgcgga cgccgacgac gtggcgacca cccccggcac
     3361 gaccaccccc ggcacgacca ccccggccca gccgccggcc gtcaccgcgc cggaggccgg
     3421 gaccgacacc ccggtcacgc tgcccgcgcc cgtcacggcc cagagcgacg acggcaccgc
     3481 ggccgccacc gacctgtccg ccgccgcgga ggccgcgctc cagaagcaga tcgacgccct
     3541 ggtgaaggcc accacctccg gcaccgccga acaggtgagc ccggccgtca aggacctgat
     3601 gaccggcatc gtcaacgtcg tcgccgccac gatggtcggc aacgggctgc ccgccgcgga
     3661 cctggccggc ctgcccggcg taccgaccgc gccggaggcc accacgcctg aggccgccac
     3721 gccggaggtc gccgcgcccg cggacccctc ctgacaccgg gcctccctca ctccgcgttt
     3781 cccgggccgt cgcacctccc tgccggaggt gggacggccc ttcgcgtgtc cggagatctc
     3841 atatgtcttc cggacggcgg agtttccggc tccggcgcag gtcgttaggg aggtcgtcag
     3901 caatacctcc ggtgacacgg gtcgccgaag aaaggaacac catgaagtcc ctcaaggccg
     3961 ccgccgtcgt cgccggttcc ctgatcgccg ccggctccgc cgcgcccgcg ttcgcctacg
     4021 acgcctccga cgccgcagcc ctcacgccca ccagcctcaa cggcgccgtg aacgcgctga
     4081 ccgcggagcc catcgacatc atgccgatca agcaccagtc ggaagggcgg tccgacgcgc
     4141 tggacaccga gaacaaggac tccgtgctgc acaccgtgaa gggcgcgacc accaagctga
     4201 actccgcgaa cggcctgctg ggcggcctcc cgctgggcgg ctgagcctcc ccgctccccg
     4261 ccgtacctcc gccctaactc cgccgtacct ccgccttccg gagcagtctc tcccgccttc
     4321 ccgaagccgt acctcccgcc ttctcgaaag ggaccgtgcc tgtcatgaag ccgaccacca
     4381 gacgaaccct ggccgccgtg gtcaccggtg tggccgccgc cgtgggggtg gccgcgaccc
     4441 ccgccgccgc ggcccgcgcc gttcccgtgc cggtgccgct gggcggcgcg gagacggccc
     4501 tcggcatgga actgcccgag gtggcgggcg agctgcccgt gccgacggcg ggcgcaccgg
     4561 agggaccgcg gtatgtcgag gggcggctgc tgccggagcg gacgctgccg cagctgccgg
     4621 tgcgcggcgg gctgcccggc gtcgacgtgc gccagccgct gccgcacatc ctcggcgacg
     4681 acttcgacca cgccggtgtc gacgcccccg ccgccgagct gcgcaccctg gcgcccgggc
     4741 tgtccctgga cgccccgctc accgcgccga accccgaggc cctcggcctg ccggcgccga
     4801 agcttcccga ggtcggtgtg ctcgccccgg tggtccagac ggttccggtg gccgacctgg
     4861 gcgtcggacc gggccggtaa cccgcgcggc ggcctcgtgc gaccgggcgg tgctgctccg
     4921 actggccctg cgtgcggtga ccgaggggcc cgccgtccgg ttagcggtgc ggaccaacgg
     4981 aaccggacga ggagtgagcc atggccggca acacgggcgc gacggtgaac ggcgcgacgg
     5041 agagaggcac ggagcgggcg tacgcggcgg aggatacggc gagcggcggg ccctcgtccg
     5101 gtacgcggag gcaggtgatg cgggggctgc tcgccccggc cctcgccgtc ggcctggcgc
     5161 ccctgatcgc cgcctctcgg ccctccggag ccgccgaggc cgccgaggcc gcgacgaccg
     5221 gaccgcgacc cgcccgcccc gggcaccccg ggccgcacgc cgtcgagtcc gcccagtccg
     5281 ccgagtccgc cgacggggcc gagagcaccg ccttcgacga gacctaccgg ggccggcgca
     5341 tccgcggagt ccgctccgcc gcgggccggg cggtgggcgc cggcacctgg caggtcaccg
     5401 tggacggccg tccgctgcac ctgatgcgcc gggccgacgg cagctggctg agcatggtcg
     5461 accactaccg gtcgtacccc accccgcttg ccgcggcccg cggcgcggtc gacgaactcg
     5521 gccccggcga gcacctgcgc gacaccccgt ccatcggcca cgaccagcac tccggggggc
     5581 ggcatggcgt acacgcgtaa ggacgtcagc acgctgaccc gcaccgagaa gcgacggttc
     5641 gtgaacgcgc tgctggagat caaacgacgc ggcgagtacg acgagttcgt gcgcacccac
     5701 atcgagtact acgtctccga cggcgaggac ggactgcgca cggcgcacat ggcaccgtcc
     5761 ttcctgccct ggcaccgccg gttcctgctg gacctggaag aggcgctgcg ccgggtggac
     5821 gacgcggtca ccgtgccgta ctgggactgg accaaggacc gcggcgccaa gtcgacgccc
     5881 tggaccgccg acctcctggg cggcaccggg aggcgttccg accaccgggt caccaccggt
     5941 cccttcgccc acgccggggg caactggacc ctcaaggtga acgtcaccga cacggagtac
     6001 ctcacccggg acctggggcg cgcgggcgat cccctcggcc tgccctcgaa gagcgacctg
     6061 gagtgggcgc tggacgaccc ggcgtacgac acctcgccct acgactccac cgtgcgcaag
     6121 gggttccgca acaagctgga ggggtggggg accgggcggg gcgaggtctc ctggcgcaac
     6181 cacaaccgcg tgcaccgctg ggtcggcggc gccatggtcg gcggcgcctc cgtcaacgac
     6241 ccggtgttct ggctgcacca cgccttcgtc gacctccagt ggtcgcgctg gcaggcccgc
     6301 caccgcggcg cccgctacct gcccgccgag ccgcccggcc ggggcagcgc gcagcgcggg
     6361 cggatcgtcg cccggcacga gaggctgccg ccgtgggacg tgacaccgga cgagctggag
     6421 gacgtcggcc ggatctaccg gtacgcctga gcgcgtccgt cccggacacg gcgacacggg
     6481 gacacgggga cgagttcggg cgcgggcccg agcgcgggag cccgggtacg ggggcccggg
     6541 tacgggagcc cgagtgcggg aagagccccg gccgggaagg ccggggctcc tcggtgcctg
     6601 gggggactca gccgccgtag ccgccgctct tgtcgccgtc gtcgtcgccg ttttcgcagg
     6661 cgttgccgaa ggccgggttc agcaggccga tcacgtcgat ggtgttgccg cagaggttga
     6721 ccggcacgtg caccgggatc tggacgacgt tgccggacag gacaccgggc gagccgacgg
     6781 ccgcgcccgc cgcgcccgcg tccgccatgg ccatgccggt gccgctgagc accacggcac
     6841 cggcgccgag ggcgacgccg gcggccttcg cgatgcgaga catcacgttc tccttctgac
     6901 tcggggagcg cggcggcagg acgcgccgcc gcactgccgg ttcaacgcac ccggtcgcgg
     6961 cgggtcacgg ggggaatgtg tggatcaccc tttttgagcg tccggggcgg ggccggaaaa
     7021 accgttctcg tgcgcccccg tgggtccact agcctcccgg catgccgaat gaccgatccg
     7081 actcgcccgt cgaggatccc gagtcgtccc agccgcccca gcagtcgcag ctgtcccagc
     7141 agccgcagct gtctcaatcg tcccagtcgt cacagccgct ccagccgctc ccgccgctcc
     7201 agccgtcccc ggacaccggc gcggcgggcg gcgacgcccg ggacggcatg atcgtgcgga
     7261 ccttcgtcag ggacggccgg ctggtgcgtc tccccgcacg atggacgcgc aagaaggtgg
     7321 tgctgcggca catcgccgag cagaccttcg tgcccggtgt ggagtacccg gaacgcaccg
     7381 tcgacgagaa gctgcgcgcc tggtgcgagg acggcgaggt cgaccatgtg acgctccggc
     7441 gccacctggt ggacttcggt cacctgcgcc ggagcgacgg aatctacgcg cgcccggcgg
     7501 actccgcccg gacgggctcc gcctgagcgg ccctacgccg ggttcggtgc cgcaggcgcc
     7561 ggcaccggca gccgccgtac gggggaaccc gcgaggtagg cggcgatcgc ctcgacggcc
     7621 tggccgtagt acgtggcgta gttggcgcgc gagacatagc ccaggtgggg cgtcgcgagc
     7681 aggcgcggag cggtgcgcat cgggtggccg gcgggcagcg gctcggtgtc gaagacgtcc
     7741 acgcccgcgc cggcgatgcg gccctcgtgc agtgcggcga gcagcgcctc ctggtcgacg
     7801 atcgccgcgc gcgaggtgtt gaccaggtag gcggtcggct tgagcagggc cagttcggcg
     7861 gggccgagca ggccgcgggt gcgctcgccc agcgccaggt ggacggagac gaagtcggcg
     7921 gtgctcagga gctcttcctt ggaacgggcc agctcggcgc cgacttcgtc ggtgcgctcc
     7981 cgggtgaggt tctggctcca ggcgctcacc cgcatgccga aggccaggcc gacctgtgcc
     8041 acccgcccgc cgatcttccc caggcccagc aggccgagcc ggcggccgtg caggtcggcg
     8101 cccaccgtct gctgccaggg gccgccctcg cgcagtccgt tgccctcctg gacgatgccg
     8161 cgggccagac ccagcagcag ggcccaggtc agctcgacgg gcggggtgga ggaggaggcc
     8221 gtaccgcaga cggtgacgcc gtgcgcctcg gcggcggcgt agtcgatcac gctgttgcgc
     8281 atgccggagg cgacgaggag cttcagccgg ggcagccggg cgagcaacga gccggggaac
     8341 ggcacgcgtt cgcgcagggt gaccacgatg tcggcgtccg cgagcgccgc ggcgagggcg
     8401 tcctcgtccg ggaagtgggt ggcgtaggac acgacgtcca cgtcgtccgc gaccggggac
     8461 cagtccgcga tctcggtcgc cacctgctgg aagtcgtcga gtaccgcgca gcgcagtcgc
     8521 acgtcgtcca gtccctcccc gcccggcaag cggtggcgtc ccggcgggcg ccacgatcgg
     8581 cagccgatcg tgtcacaccc ggaccggggc gtgtcacacc cggaccgggg aacgggccgt
     8641 tgcggtcgct cgggccgttg atcaacccgt caacacatga gtgagcgccc ccggcaggac
     8701 tcgaacctgc ggccaagcgc ttagaaggcg cctgctctat ccactgagct acgggggcct
     8761 gatgtggggc ctcggtcctg gtgtccggct gtgaaccggt ccgtgacctc gccggggaca
     8821 aggatagggc tcccggctcc atgtcccgga tgcttcccct ccgtggctcg atgtggaggt
     8881 tcggtgaagc gatcccgata atcgcaggca ggtacgaatc gtgcatcgtt tttggcgcct
     8941 cgcgcatcgg gtgttgtcca ctcgttatgc ctggactgtc ctcgcgtccc agtagtccca
     9001 tccgcccgga gtgtccgatc ggcgcgcaga catgctcatc atgctcatat gcttcagaat
     9061 tccctcaaaa ttgggcattc ttcacatgtg gtgaccttgg acgtacggcc tcagctgctc
     9121 gacgcactct ccgccctgcg cgaccgtgtc gcggccgcac gcttcccgct gcccctggcg
     9181 ggggccccac gcgcgcgtgc caaccgcgac gaactgctcg cccagctcga cgactacctg
     9241 atgccccgcc tgaaggatcc cgaggcgccg ctgctcgccg tggtgggcgg atccaccggc
     9301 gccgggaagt ccaccctggt caactccctg gtgggacggc gggtcagcga ggcgggtgtg
     9361 ctgcggccca cgacacgcac gccggtcctg gtgtgccacc cggaggacca tcactggttc
     9421 agcggcatgc gggtgctgcc cgacctcacg cgcgtgtggg tgccccaccg ggaccccgac
     9481 gacgacctgc tgctgcccgg cgagaacccc gcgcgcgtgc tgcgggtcga gaccgccgac
     9541 accctgccgc ccggcatcgc cctcctggac gcgcccgacg tcgactccct cgtcgccgac
     9601 aaccgcgtcc tggcggccga actgatctgc gccgccgaca tctgggtgat ggtcaccacg
     9661 gccgcgcggt acgccgacgc ggtgccctgg cacctgctgc gcaccgccaa ggagtacgac
     9721 gccaccctgg tcaccgtgct cgaccgggtg ccccaccagg tggtgtccga ggtgtcgcgg
     9781 cagtacgggg cgctgctcac caaggccggg ctcggcgacg tcccgcgctt caccgtcccc
     9841 gaactgcccg agtcggcctg gggcggcgga ctgctgccgg ccagcgccgt ggcgccgctg
     9901 cgctcctggc tcgtccaccg ggcgcaggaa ctcgacgccc ggcaccacgc catggcccgc
     9961 acggcgcacg gcatcctgga ctcgctcaag tcccggatgc ccgagctggc cggagccacc
    10021 gccgcacagt acgccgctgc gctgcggctc accgccgccg tcgacggcgc ctacgacgat
    10081 gagcacgcgc gcgtgcgggg gcggttgcag gcgggcgcgg tgctcgccgg ggacgcgctg
    10141 aagcgctggc gggccttccc cctggactgt tctccggaag agctcctcga ctcgctggtg
    10201 gagagcctga gcgccctgct gctgtgcgcc gtcacggctg ccgacgagcg ggtcgacgag
    10261 gcctggcgcc acgaaccggc ggcggacgcc cccgaactcg cgggccacgc ccccgcgccg
    10321 gagagcgccg agcaccgcat cgggctggcc gtacggcgct ggcggcgcga gctggaggag
    10381 tacgccgagg aggaggtgcg cgagctggac cggaacctgg caccggaccc cggggtggtg
    10441 gccggcctgg tcgccaccgt gctgctgggc gggcggcgcg cgcgtgtcgc cggcgagcgg
    10501 ctcgccgaga ggatcggcgc ccacggcgcg ctgcggctgc gcgaccgggg cgggcggctg
    10561 ctcgaagaac acgtcgaccg ggtgatgcac gtcgagcgcg
//
