LOCUS       NZ_KZ195574            11320 bp    DNA     linear   CON 06-SEP-2024
DEFINITION  Streptomyces sp. CS113 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ195574
VERSION     NZ_KZ195574.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS113
  ORGANISM  Streptomyces sp. CS113
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8695358)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8695358)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8695358)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 78.17x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188365.1-RS_2024_09_06
            Annotation Date                   :: 09/06/2024 02:31:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,790
            CDSs (total)                      :: 7,719
            Genes (coding)                    :: 7,614
            CDSs (with protein)               :: 7,614
            Genes (RNA)                       :: 71
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 35 of 105
            Pseudo Genes (incomplete)         :: 81 of 105
            Pseudo Genes (internal stop)      :: 4 of 105
            Pseudo Genes (multiple problems)  :: 15 of 105
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:56:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1998990
            Orig. end                         :: 2010310
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195574.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
FEATURES             Location/Qualifiers
     region          1..11320
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="14"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11320
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11320
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6320](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6320
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(538..558)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
     misc_feature    1174..1189
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.16"
                     /tool="antismash"
     gene            1391..2275
                     /locus_tag="B9W62_RS08690"
                     /old_locus_tag="B9W62_08700"
     CDS             1391..2275
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030618.1"
                     /locus_tag="B9W62_RS08690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_08700"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_087806623.1"
                     /transl_table=11
                     /translation="MISLVRRATALCVLGAALTACGTTRAEVAPRPAPSAPAPSPSPSS
                     SRTPTPTLAPGPAGLTPVFEHGPRTGAAKDGAEDGKLVALTFDADMTADQGPRAASGER
                     FDNPGLIAALRELKVPATVFMTGRWAEEYPDEARSIGRDPRFEIANHSYSHYAYTDDCY
                     GLPTVSGDRMRSDLERAYTAFEKAGVPNPMPYFRFPGGCYDEAALKKLSATGVTAVQWD
                     VVSGDAFATDADAVTRQVLDGVRPGSVVVMHCTRSAAPVTEEVVRAVVPELRRQGYRFA
                     KVSELIGAANGHA"
     misc_feature    2283..2301
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 20.62"
                     /tool="antismash"
     gene            2293..2520
                     /locus_tag="B9W62_RS08695"
                     /old_locus_tag="B9W62_08705"
     CDS             2293..2520
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972872.1"
                     /locus_tag="B9W62_RS08695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_08705"
                     /product="hypothetical protein"
                     /protein_id="WP_087806624.1"
                     /transl_table=11
                     /translation="MNSDHGQNPDGADGAGCEPGGYCLIDAPRPPKADGPPYAECVQCR
                     EPTEYPESYKGITLCPVCEWQEAQRTACSG"
     gene            complement(2512..3186)
                     /locus_tag="B9W62_RS08700"
                     /old_locus_tag="B9W62_08710"
     CDS             complement(2512..3186)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972873.1"
                     /locus_tag="B9W62_RS08700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_08710"
                     /product="ABC transporter"
                     /protein_id="WP_087806625.1"
                     /transl_table=11
                     /translation="MTALLRYQTALLLRSQRWLPPVVLYAAALGIGVQGGQPVLDSLGW
                     AAAVLLPVAAWLVRICVTSEPPAARSCVSAARGPGRAHLAAVLAALAVAVVLGAVATVV
                     VTLISDPVSTGHGTRVPPLPAAGAGLLAALVCALLGTAVGALTAWPVLRSPGRAVPALL
                     LAALLALVASGSPARAAVGDLVAGSKSGAVPLPLLPLAGAAVIAVAAVAAACALGSRRP
                     SA"
     gene            complement(3183..4069)
                     /locus_tag="B9W62_RS08705"
                     /old_locus_tag="B9W62_08715"
                     /pseudo=""
     CDS             complement(3183..4069)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972874.1"
                     /locus_tag="B9W62_RS08705"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_08715"
                     /product="ATP-binding cassette domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRRELRLDGVGRRYGLRGPWVLRGVELGVPPGALVRVEGANGTGK
                     STLLRLLAGIDAPTEGRVTGRPRTAYVPERFPAALPFTAARYLTHLGAVHGLSRRAATR
                     AARQWLERLGAAGYADTPLTRLSKGSSQKVAVAQALLAEPELLVLDEAWTGLDADARAE
                     LERAVAVGVPPRPSGSGGGRRPAVPSSSSTTIRAAWAGRATPCTSCATAPCTAVPDRNR
                     YRPGRGSSSGCRVRPVPPCPATCAGRPRRRRRPAPVPTRSPSPPRTPTSCCAPC"
     gene            4114..4863
                     /locus_tag="B9W62_RS08710"
                     /old_locus_tag="B9W62_08720"
     CDS             4114..4863
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030616.1"
                     /locus_tag="B9W62_RS08710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_08720"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_087806626.1"
                     /transl_table=11
                     /translation="MSNDPTSPVPAPVPVPDSPFRPEPGDRDLAPQFVLPLVVRIERAA
                     PPPRTDALETAARAVLVMLGDERSTGDGAWARAMRDWQDARIRKVVRRARGAEWRRAEA
                     LPGITVTGKTAEVRVFPPVPLDGWPKDLARLQVSGTDLDDPEPPADADPSAPVLWLNPD
                     LDMSAGKAMAQAGHGAQLAWWELSDGERTAWREAGYPLSVRTADPARWTGLTTGGLPVV
                     RDAGFTEIAPGSCTVVADHPALRRPRP"
     gene            5001..6320
                     /locus_tag="B9W62_RS08715"
                     /old_locus_tag="B9W62_08725"
     CDS             5001..6320
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016327585.1"
                     /locus_tag="B9W62_RS08715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_08725"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_087806627.1"
                     /sec_met_domain="DUF692 (E-value: 2.8e-102, bitscore:
                     340.3, seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRLGTGIGWRPEIADAVEEMPGIEWVEAVSENLCPGHLPGSLLR
                     LRERGVTVVPHGVSLGLGGADRPDPGRLAALAERAEVLGSPLVTEHIAFVRAGGALTAS
                     PPLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALVSWPDEELTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNLGEDPAEALAELPLEAIAYVHVAGGFERDGVWHDSH
                     AHPVARPVLDILTDLASRVAPPGVLLERDENFPDGDELRGEVEAIRAAVEKGRAAATGT
                     GAAARTPRTGTDAASGADDAVRQRLGLAQAALLSALVAGTPAPEGFDRVRLGVQARALA
                     GKRADVVAKVAPELPVLLGARYRPAFLAYAQGCPMTGGYRRDALGFVEHVLRTPPGEGE
                     DPGVRRELREWWLERSGPVPRSARPGVRLARATRRVLLRR"
     gene            6617..7837
                     /locus_tag="B9W62_RS08720"
                     /old_locus_tag="B9W62_08730"
     CDS             6617..7837
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1192: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449240.1"
                     /locus_tag="B9W62_RS08720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_08730"
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="WP_087806628.1"
                     /transl_table=11
                     /translation="MAQAGADEKVGHARYTYRLRVSSAARALLAAEWGRCRWLWNECVA
                     KSRAVHLHNRATGEKTTCGPAQLDRMLTEARSRTPWLREGSSVPQQQVIRNFGRSRAKA
                     HKDIAERQPVARRAGMPTWKTRRESLATLNYTRRGFRLKDGRLHLTGGIVVRAVWSREL
                     PAEPSSVRVYQDSLGHWYASFVVPAQVQPLPATGRVLGVDWGVRETATTTTDAHDLPHS
                     SHGGKAKEKLTRYDRMMARRRPKKGTAASRGYREAKRLRAKAYKKVARQRADTGRKWAK
                     KVVRDHDAVAVEDFRPKFLARSTMARKAADAAIGATKAALIEMGRKHGRDIRLVHPAYT
                     TMDCAQCGARAKHALPLGERTHTCTACGTTSPRDKNSARVMLARAGLNPAGAEGGRPPG
                     APLQEAA"
     gene            7949..8746
                     /locus_tag="B9W62_RS08725"
                     /old_locus_tag="B9W62_08735"
     CDS             7949..8746
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972877.1"
                     /locus_tag="B9W62_RS08725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_08735"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_087806629.1"
                     /transl_table=11
                     /translation="MFWVLFLLSAWAVAGLACLRLCLAAVRAAAVDPRAAAPEHTLTLY
                     EAAFLSGGPRRVADLTLVSMARQRRLLLAHTGWATVVDPCGRDDMERSVIGAIGPGGQS
                     RIAPVRAAAAAAEAVRSLADRLVGAGLAVPDGGAGGVAAGVRRVRVAAVTVCALGLVAL
                     ALPLPATEPRLLIALWFALPLALTLGCLAITRMEIHPYARWASPAGQRLVGALVRQADG
                     TADDRTFLTSVAVRGVHAIGEPDLRAAFAHREKYAGDHGERRA"
     gene            8871..10454
                     /locus_tag="B9W62_RS08730"
                     /old_locus_tag="B9W62_08740"
     CDS             8871..10454
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (110-291). E-value:
                     3.2e-17. Score: 54.9. Matches aSDomain:
                     nrpspksdomains_B9W62_RS08730_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006380016.1"
                     /locus_tag="B9W62_RS08730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_08740"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_087806630.1"
                     /sec_met_domain="PF00561 (E-value: 1.9e-22, bitscore: 78.9,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALYSAAGSLLLTTLAATPAQSAPPAGPGSAEAHGTAVAARR
                     AAAAGIEFGRCPKAEDLPGGIRCGTVSVPLDYAHPDGKRIELTVSRMRATHQDPAGGER
                     EVPGRGALLYNPGGPGASGLYFPLVGMVPEWKRIAAAYDLVGYAPRGVAPSAPLSCQDP
                     GDFFKGPRPAPTRPSDAYKEQRAAEAEAYARDCAERGGDALRHYHSLNNARDLEVVRAA
                     LGERRLTFMGASYGTYLGALYAELFPSHVRRMVFDAVVNPDPEQVWYRNNLDQSEAFED
                     RWDDFKEWVAGHDAVYGLGDTARQVQDAYEEASARLAAEPAGGTVGPAQLQGAFLKAGY
                     YDDYWPHRAEALSAYLEGDPQPLIDQAGTRTAEGAAGAENSNAVYTAVECNDAPWPTDF
                     GVWDRDNTRLARRAPFETWDNVWTNLPCAYWRAPRQEPLDVRTAPGELPPTLVLAAERD
                     AATPYEGALELRRRLAGSVLVTERDAGTHGIAGGPNDCVNGHLEAYLLEDRLPARDTSC
                     APRPEPEPRA"
     aSDomain        9201..9743
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9W62_RS08730_Abhydrolase_1.1"
                     /evalue="3.20E-17"
                     /label="B9W62_RS08730_Abhydrolase_1.1"
                     /locus_tag="B9W62_RS08730"
                     /protein_end="291"
                     /protein_start="110"
                     /score="54.9"
                     /tool="antismash"
                     /translation="ALLYNPGGPGASGLYFPLVGMVPEWKRIAAAYDLVGYAPRGVAPS
                     APLSCQDPGDFFKGPRPAPTRPSDAYKEQRAAEAEAYARDCAERGGDALRHYHSLNNAR
                     DLEVVRAALGERRLTFMGASYGTYLGALYAELFPSHVRRMVFDAVVNPDPEQVWYRNNL
                     DQSEAFEDRWDDFKEWVA"
     gene            complement(10455..11186)
                     /gene="hemQ"
                     /locus_tag="B9W62_RS08735"
                     /old_locus_tag="B9W62_08745"
     CDS             complement(10455..11186)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="B9W62_RS08735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_08745"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_003972879.1"
                     /transl_table=11
                     /translation="MSDDASTPAAERIPNKGKLAKDLNEVIRYTLWSVFKLKDTLPEDR
                     AGYADEVQELFDQLAAKDVTIRGTYDLSGLRADADLMIWWHAETADQLQEAYNLFRRTK
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRNYISVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELHRIVDLMRHLRGSEARRHVR
                     EEIPFYTGRRKDIGELVAGLA"
     misc_feature    complement(11233..11248)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.08"
                     /tool="antismash"
ORIGIN
        1 ccaagctgac cgcccagcac accctggtct acgacaagct gcgccgcacc cgcggccccg
       61 agggcgcccg cctgtacgcc cgctcgcagt ccgaggcgat cgagcgcgcc gccgggatcg
      121 tcgcggagct gagcatcgac tgcgactggg agacgcggga cgcgtacacc tacgtccgcg
      181 accccggccg ggtggacgag gtgcgggcgg aggcggcggc cgcgaaggag gcggggctcg
      241 cggcgtcgtt cgtgacggag accgggctgc cgttcccggt ggcgggcgcc gtgaaggtga
      301 ccgggcaggc ccagttccac cccctcaagt acctgcgggc gctcgccgcg gacctggagg
      361 cgcacggcgg gcggatcttc gaggacacca ccgtgcaggg cctggacgag ggcgagccgt
      421 gccgggtgcg gaccgaggcg ggggcgacgg tcaccgcccg ggacgtcgtg gtcgccacgc
      481 actacccgat cttcgaccgg gcgctgctct tcgcccgcct ctccccgcgc cgcgagctgg
      541 tcgtcgccgg gacgatcgag gcggaccgcg acgtcgacgg catgtacatc acgccggagg
      601 acaacacccg ctcggtgcgt accgcgcccc tggacgacgc gggccgccgc ctgctcgtcg
      661 tcaccgggga gcacttcacc cccggcacgg gcgacacccg cgagcgcttc gcgcgcctgg
      721 ccgcctgggc ggacgagcac ttccccggcg tcgagcgcac ccacgcctgg gccacgcagg
      781 acatcgaccc gaccgacacc gtgccgatgg cgggtccgct gcacccgggc gcgcaccaca
      841 cctacgtcgc caccggcttc ggcggctggg gactgagcgg cggcatgatg gcgggccggc
      901 tgctcaccgc gcagatcacc ggcgaggagt gcgcctggag cgggctgtac gacccgcgcc
      961 ggctgcgcac ggcggtgcgc gaggcgcccg cgctgctcaa gacgcaggcc gaggtggccc
     1021 ggcacttcgt cggcgaccgg ctgcggcccg cgccgccggt ggacgcgctg cctcccggtg
     1081 agggcgcggt ggtccgggcg ggcggcgacc ggctcgcggt ctaccgggac gaggccggcg
     1141 ccctgcacgc cgtctcgccg cgctgcaccc acctgggctg cctggtcgac ttcaacgcgg
     1201 ccgagcgcgc ctgggagtgc ccctgccacg gctcccggtt cgccacggac ggcacggtgc
     1261 tggagggccc ggcgaccaag ccgctggagc ggcgggacat ctgacaccgg ctggaccggg
     1321 cgggtgagag cggcggcacg tcgacggacg tagtcataca aaccgaacat cccacttcta
     1381 cggtcgtacg gtgatctctc tcgtacgacg tgccaccgcg ctctgtgtcc tgggcgccgc
     1441 cctcaccgcc tgcggaacca cgcgcgccga ggtggccccc cgcccggcgc cctcggcccc
     1501 cgccccgtcc ccgtccccgt cctcctcccg gacgcccacg cccaccctcg cgcccggccc
     1561 ggcgggcctg acccccgtct tcgagcacgg cccgcgcacg ggcgccgcga aggacggcgc
     1621 ggaggacggc aagctggtcg ccctcacctt cgacgccgac atgaccgccg accaggggcc
     1681 gcgggcggcg tcgggcgagc ggttcgacaa cccggggctg atcgcggcgc tgcgggagct
     1741 gaaggtgccg gccacggtgt tcatgacggg ccgctgggcc gaggagtacc cggacgaggc
     1801 ccgctccatc ggccgggacc ctcggttcga gatcgccaac cactcttaca gccactacgc
     1861 ctacaccgac gactgctacg gcctgcccac cgtgtccggg gaccgcatgc gctccgacct
     1921 ggagcgggcc tacaccgcct tcgagaaggc cggggtgccg aacccgatgc cgtacttccg
     1981 cttccccggc ggctgctacg acgaggcggc cctcaagaag ctgtcggcca ccggggtcac
     2041 cgccgtgcag tgggacgtgg tgagcggcga cgccttcgcc acggacgccg acgcggtgac
     2101 ccggcaggtg ctcgacgggg tacggccggg ttcggtcgtc gtcatgcact gcacgcgcag
     2161 cgccgccccg gtgaccgagg aggtggtgcg ggcggtggtg ccggagctgc gccgccaggg
     2221 ctaccgcttc gcgaaggtct cggaactgat cggcgccgcg aacgggcacg cctgacgtcc
     2281 tacgctggag acatgaacag cgaccacggc cagaacccgg acggcgcgga cggcgccggc
     2341 tgcgagcccg gcggctactg cctgatcgac gcgccccggc cgccgaaggc cgacggcccg
     2401 ccgtacgcgg agtgcgtgca gtgccgggag cccacggagt acccggagtc gtacaagggc
     2461 atcacgctct gcccggtctg cgagtggcag gaggcgcagc gcaccgcctg ctcaggctga
     2521 cggcctgcgt gagccgagcg cacaggccgc ggcgaccgcg gccacggcga tgaccgccgc
     2581 cccggccagc ggcagcagcg gcagcgggac cgcgcccgac ttcgacccgg cgaccaggtc
     2641 gccgaccgcc gcccgcgccg gggaccccga cgccaccagc gccagcaggg ccgccagcag
     2701 cagggcgggg accgcgcgtc cgggcgagcg cagcaccggc caggcggtga gcgcgccgac
     2761 ggcggtgccg agcagggcgc acaccagcgc ggcgagcagt ccggcaccgg ccgccgggag
     2821 cgggggcacg cgcgttccgt gaccggtact gaccgggtcg ctgatcagcg tcacgaccac
     2881 ggtcgcgacc gcgcccagga ccacggcgac ggccagcgcc gcgagcacgg ccgccaggtg
     2941 ggcccgcccc gggccccgcg ccgccgacac gcaactccgc gcggcgggcg gctcggacgt
     3001 gacgcagatc cgcaccagcc aggccgcgac cggcagcagc acggccgccg cccagcccag
     3061 cgagtcgagc acgggctggc cgccctgcac cccgatgccg agcgcggcgg cgtacaggac
     3121 gacgggcggc agccagcgct gggagcgcag caggagggcc gtctggtagc gcaggagagc
     3181 ggtcatcggt gctcctccgg ggcgtgcacg ctcaccacgt gccagggcgg ccgggcggtc
     3241 agcagggtgc gcagcaggac gtcggagtgc gaggcgggga cggtgagcgt gtgggaaccg
     3301 gggccggtct cctgcgccga cgcggccgtc cggcgcacgt cgccgggcag ggcggcaccg
     3361 ggcggaccct gcacccggac gacgacccgc ggcccggccg gtaccggttc cggtccggta
     3421 cggcggtgca gggtgccgtc gcgcacgacg tgcacggcgt cgctcgcccc gcccaggcgg
     3481 cccggatcgt ggtcgacgaa gacgacggca ccgccggccg ccgtcctccc ccactgcctg
     3541 aagggcgtgg gggtaccccc acggccaccg cccgttccag ttccgcccgc gcgtcggcgt
     3601 cgagcccggt ccaggcctcg tcgaggacca gcagctccgg ctcggcgagc agcgcctgcg
     3661 ccaccgccac cttctggctg ctgcccttgg acagcctggt cagcggggtg tcggcgtacc
     3721 ccgcggcgcc cagccgctcc agccactgcc gcgcggcccg ggtcgccgcc cggcgggaca
     3781 gcccgtggac ggcgccgagg tgggtgaggt accgcgcggc ggtgaagggc agggcggccg
     3841 ggaagcgctc ggggacgtag gccgtgcgcg gacggccggt gacccgcccc tcggtggggg
     3901 cgtcgatccc ggcgaggagg cgcagcaggg tcgacttgcc ggtgccgttg gcgccttcga
     3961 cgcggacgag ggcgccgggc ggcacgccca gctcgacccc gcgcagcacc caggggccgc
     4021 gcaggccgta gcggcgtccc acaccgtcca gccgcagctc gcgtcgcatg gggccatact
     4081 ctcccagccg cgcgggttgg cagactgtag gacgtgagca acgatccgac gtcccccgtc
     4141 cccgcccccg ttcccgtccc cgacagcccc ttccgtccgg agcccgggga ccgcgacctc
     4201 gcgccgcagt tcgtgctgcc gctggtggtg cgcatcgagc gggcggcgcc gccgccccgt
     4261 accgacgcgc tggagaccgc ggcccgggcc gtgctggtga tgctcggcga cgagcggtcg
     4321 accggcgacg gcgcgtgggc gcgggcgatg cgggactggc aggacgcccg catccgcaag
     4381 gtcgtgcggc gggcccgggg cgcggagtgg cggcgggccg aggcgctgcc cggcatcacg
     4441 gtcaccggca agaccgccga ggtgcgggtc ttcccgcccg tccccctgga cggctggccc
     4501 aaggacctgg cccgcctcca ggtctccggc accgacctcg acgaccccga gccgccggcc
     4561 gacgccgatc cctccgcgcc ggtgctctgg ctcaacccgg acctcgacat gtcggcgggc
     4621 aaggcgatgg cgcaggcggg ccacggcgcg cagctcgcct ggtgggagct gtccgacggc
     4681 gagcggacgg cgtggcgcga ggcgggctac ccgctgtcgg tgcgcaccgc ggacccggcc
     4741 cgctggaccg gcctcaccac gggcgggctg ccggtggtgc gcgacgccgg gttcacggag
     4801 atcgcaccgg gttcgtgcac ggtggtggcg gaccatccgg cgctgcggcg gccgcgcccg
     4861 taggcggcgc gggcgcgccg gccggtgccc ggggtggcac gcgaacctca aatgttcctc
     4921 tgaaggcgct cgccgcgggg ttggggcccg gtcgcacggg cgataccccg gtcatgacga
     4981 cgcggaggga gggatcggcc atgcgacgac tgggcaccgg catcggatgg cggccggaga
     5041 tcgcggacgc cgtcgaggag atgcccggca tcgagtgggt cgaggccgtg tccgagaacc
     5101 tgtgtcccgg gcatctgccc ggctcgctgc tgcgactgcg cgagcgcgga gtgaccgtcg
     5161 tgccgcacgg ggtctcgctc ggtctcggcg gcgcggaccg gccggacccg gggcggctgg
     5221 cggcgctggc cgagcgggcc gaggtgctcg ggtcgccgct ggtcaccgag cacatcgcct
     5281 tcgtgcgcgc gggcggggcg ctgaccgcgt cgccgccgct ggaggcgggg cacctgctgc
     5341 ccgtgccgcg cacgcgcgac gccctcgacg tgctgtgcga gaacgtgcgc atcgcccagg
     5401 acgcgctgcc cgtgccgctg gccgtggaga acatcgccgc gctcgtctcc tggccggacg
     5461 aggagctgac ggaggggcag ttcctgtacg agctggccga ccggaccggc gtgcggctgc
     5521 tgatcgacgt ggcgaacctg cacaccaacc acgtcaacct gggtgaggac ccagccgagg
     5581 cgctcgccga gctgcccctg gaggccatcg cctacgtcca cgtcgcgggc ggcttcgagc
     5641 gggacggcgt ctggcacgac agccacgccc accccgtcgc gcggccggtg ctggacatcc
     5701 tgaccgacct cgcctcgcgc gtcgcgccgc cgggcgtact gctggagcgg gacgagaact
     5761 tccccgacgg cgacgagttg cggggtgagg tggaggcgat ccgcgccgcc gtggagaagg
     5821 gccgggcggc ggccaccggg accggagcgg ccgcccgcac cccgcgcacc ggtaccgatg
     5881 cggcctccgg cgccgacgac gccgtacggc agcggctcgg gctggcgcag gccgcactgc
     5941 tgtccgcgct ggtggcgggg acgccggcgc ccgaggggtt cgaccgggtg cggctggggg
     6001 tgcaggcgcg ggcgctcgcc gggaagcggg ccgacgtggt cgcgaaggtc gcgcccgaac
     6061 tgccggtgct cctgggcgcc cggtaccgcc ccgccttcct cgcctacgca caggggtgtc
     6121 cgatgaccgg cggctaccgg cgggacgccc tcggcttcgt cgaacacgta ctgcggacgc
     6181 caccggggga gggcgaggac ccgggggtgc ggcgggagtt gcgggagtgg tggctggaac
     6241 ggtcgggacc ggtgccgcgg tcggcccgcc ccggggtccg gctggcccgt gcgaccaggc
     6301 gcgttctgct gcggcgctga atcccgctcg gcagcgtggg gacatcgcca acggacatca
     6361 ccctcttgca aggaggctcc ggcatccacg ccgaggagga agcacatccg gatggtgcga
     6421 cgcggagcgt cgccgtggtc cgcttcggcg cggagcgccc ggcgtaccca aggtagtgcg
     6481 tacatgtgtg cccatagtcg ctggtcgggg tggtggttat gggatcggcg ggtgtgtgtg
     6541 cggctggcgt acgtatggtc ctttgcgggg ttcgggacgg cacggtgtcc tggccacagg
     6601 tcgtgaggtg ggtgagatgg cgcaggcggg ggccgatgaa aaggtcgggc atgcccggta
     6661 cacgtaccgg ctgcgggtgt cgtccgcggc ccgcgccctg ctggcggccg agtggggccg
     6721 gtgccgctgg ctgtggaacg agtgcgtcgc caagtccagg gccgtacacc tgcacaacag
     6781 ggccaccggc gagaagacca cgtgcggccc ggcgcagctg gacaggatgc tgaccgaggc
     6841 ccgttcccgt acgccgtggc tgcgggaggg ctcctcggtt ccccagcagc aggtcatccg
     6901 caacttcggc cgctcccgcg ccaaggcgca caaggacatc gcagagcggc agcccgtggc
     6961 gcgccgggcc gggatgccga catggaagac caggcgtgag tcgctggcga ccctgaacta
     7021 cacccggcgc ggtttccggc tgaaggacgg ccgactgcat ctgacgggcg gcatcgtcgt
     7081 gagggcggtg tggtcgcggg agttgccggc cgagccgtcc tccgtacgcg tgtaccagga
     7141 cagcctcggg cactggtacg cctcgttcgt cgtccccgcc caggtccagc ccctgcccgc
     7201 gaccggccgg gttctcggtg tcgactgggg tgtgcgagag acggcgacga cgacaaccga
     7261 cgcgcacgac ctgccgcact cctcgcacgg tgggaaggcg aaggagaaac tcacccgcta
     7321 cgaccggatg atggcccgcc gcaggcccaa gaaggggacg gccgcatcga ggggctaccg
     7381 tgaggcgaag aggctgcggg cgaaggcata caagaaggtc gccaggcagc gtgcggacac
     7441 cggccgcaag tgggccaaga aggtcgtccg cgaccacgat gccgtagcgg tcgaggactt
     7501 caggccgaag ttcctggccc ggtcgacgat ggcccgcaag gccgcggacg ccgccatcgg
     7561 cgccaccaag gccgctctga tcgagatggg acgcaaacac gggcgggaca tccgcctggt
     7621 ccaccccgcg tacaccacga tggactgcgc gcagtgcgga gcgagagcca agcacgccct
     7681 gccgctgggt gaacgcaccc acacctgcac cgcctgcgga accacgtccc ccagggacaa
     7741 gaattccgca cgcgtcatgc tcgcccgggc tggtctcaac ccggctggtg ccgaaggcgg
     7801 aagacctcct ggagcgccgc tccaggaggc agcctgagcc aggaatcccc tcccttcagg
     7861 gaggggagga ttcaaatacc cacaacactg cgtcccggaa tgtgaaaagg gcatggcgcg
     7921 gcgaaagcct ttggcataga agtacggcat gttctgggtc cttttcctcc tgtcggcctg
     7981 ggccgtcgcg ggcctcgcct gcctgcggct gtgtctggcg gccgtgcgcg cggcggccgt
     8041 cgacccgcgt gccgcggctc ctgagcacac gctgacgctg tacgaggcgg cgttcctgtc
     8101 cggcggtccg cggcgggtgg ccgacctgac gctggtctcg atggcgcgcc agcgccggct
     8161 gctgctcgcg cacaccggct gggcgaccgt cgtcgacccc tgcggccggg acgacatgga
     8221 gcggtcggtg ataggggcga tagggcccgg cgggcagtcg cggatagcgc cggtacgggc
     8281 cgccgcggcc gccgccgagg cggtacggag cctggccgac cggctggtcg gggcgggcct
     8341 cgccgtgccc gacggcggcg ccggcggcgt cgcggccggg gtgcggcggg tgcgggtcgc
     8401 cgcggtgacc gtgtgcgcgc tcgggctggt ggcgctggcg ctgccgctgc ccgccaccga
     8461 gccgcggctg ctcatcgccc tctggttcgc gctgccgctc gccctgacgc tcgggtgcct
     8521 cgccatcacg cggatggaga tccacccgta cgcgcgctgg gcctcgcccg ccgggcagcg
     8581 gctggtcggc gccctcgtgc ggcaggccga cggcacggcg gacgaccgga ccttcctcac
     8641 ctcggtcgcc gtgcgcggcg tgcacgccat cggggagccg gacctgcggg cggccttcgc
     8701 ccaccgcgag aagtacgccg gggaccacgg ggagcgccgg gcctgacgcg gtcggccccg
     8761 ggggcgcacg gaggcactcg gcggcgacac ccgcgggcgg tgcttgcccc gcccggccgg
     8821 tgccccaaac atcccttgtg tcactccccg tgccaccgaa gggatacgcg atgcgagctg
     8881 ccgccctcta ctcggccgcc gggtccttgc tgctgaccac cctcgccgcc actccggccc
     8941 agtcggcgcc cccggccggg cccgggtccg ccgaggcgca cggtaccgcc gtggcggccc
     9001 ggcgcgcggc ggcggccggg atcgagttcg gcaggtgccc gaaggcggag gacctgccgg
     9061 gcggcatccg ctgcggcacc gtgtccgtcc cgctggacta tgcccacccg gacgggaagc
     9121 ggatcgagct gaccgtcagc cggatgcggg ccacccacca ggacccggcc ggcggcgagc
     9181 gcgaggtccc cggccggggc gccctcctct acaacccggg cggccccggc gcgtccgggc
     9241 tgtacttccc gctggtgggc atggtgcccg agtggaagcg catcgccgcc gcctacgacc
     9301 tcgtcggcta cgccccgcgc ggggtcgcgc cctccgcccc gctgtcctgc caggaccccg
     9361 gggacttctt caaggggccc cggccggccc cgacgcggcc ctcggacgcc tacaaggagc
     9421 agcgcgccgc cgaggccgag gcgtacgccc gcgactgcgc ggaacggggc ggggacgcgc
     9481 tgcggcacta ccactccctc aacaacgccc gtgacctgga ggtcgtccgc gccgcgctgg
     9541 gcgagcggcg gctcacgttc atgggcgcgt cgtacggcac ctacctcggc gcgctgtacg
     9601 ccgagctgtt cccctcgcac gtacggcgga tggtgttcga cgcggtggtg aatccggacc
     9661 cggagcaggt ctggtaccgc aacaacctgg accagtccga ggcgttcgag gaccgctggg
     9721 acgacttcaa ggagtgggtc gccgggcacg acgccgtgta cgggctcggg gacacggcgc
     9781 ggcaggtgca ggacgcctac gaggaggcga gcgcgcggct ggccgcggag ccggcgggcg
     9841 ggacggtggg acccgcgcag ttgcaggggg cgttcctgaa ggccgggtac tacgacgact
     9901 actggccgca ccgcgccgag gcgctgtcgg catacctgga gggcgacccg cagccgctga
     9961 tcgaccaggc gggcacccgg accgcggagg gcgcggccgg ggcggagaac tccaacgccg
    10021 tgtacacggc cgtcgagtgc aacgacgcgc cctggccgac cgacttcggg gtctgggacc
    10081 gggacaacac ccggctggcc cgcagggcgc cgttcgagac ctgggacaac gtgtggacga
    10141 acctgccgtg cgcctactgg cgggcgccgc ggcaggagcc cctggacgtg cgcaccgcgc
    10201 cgggtgagct gccgccgacg ctggtcctgg ccgccgagcg ggacgcggcg acaccgtacg
    10261 agggcgcgct ggagctgcgg cggcgactgg cgggctcggt gctggtgacc gagcgggacg
    10321 ccgggacgca cggcatcgcg ggcggcccga acgactgcgt caacggccac ctggaggcgt
    10381 acctgctgga ggaccgcctc ccggcgcggg acacgtcgtg cgccccgcgc ccggaaccgg
    10441 agccgagggc ctgactaggc gagcccggcg accagctcgc cgatgtcctt gcggcgcccc
    10501 gtgtagaacg ggatctcctc gcggacgtgg cgacgcgcct cggagccgcg caggtggcgc
    10561 atgaggtcga cgatgcggtg cagctcgtcg gcctcgaagg cgaggatcca ctcgtagtcg
    10621 cccagcgaga aggaggcgac cgtgttggcg cgcacgtccg ggtagccgcg ggccatcttg
    10681 ccgtggtcgg cgagcatgcg gcggcggtcc tcgtcgggca gcaggtacca gtcgtaggag
    10741 cgcacgaacg ggtagacgct gatgtagttg cgcggcgtct cgtcggccag gaaggccggg
    10801 atgtgcgagc ggttgaactc ggcggggcgg tgcagcgcca tgttcgacca gaccggctcc
    10861 agcgcacggc ccagcttcgt gcggcggaag aggttgtacg cctcctggag ctggtcggcg
    10921 gtctcggcgt gccaccagat catcaggtcg gcgtcggcgc gcagaccgga caggtcgtag
    10981 gtgccgcgga tcgtcacgtc cttcgcggcc agctggtcga acagctcctg gacctcgtcg
    11041 gcgtacccgg cgcggtcctc ggggagggtg tccttgagct tgaagacgga ccagagggtg
    11101 tagcggatga cctcgttgag gtccttggcc agcttgccct tgttggggat gcgctcggcc
    11161 gcgggggtgg aggcgtcgtc actcatggcc ctattctccc gctccgccgt gcaggctctg
    11221 caccgggtgg gccgtgagct gctccacacc ccgcaggtcg ccccggatct ggtcgacggc
    11281 cgcgtacgcg ctcgcgatgc tggccgggat gccgacgccg
//
