LOCUS       NZ_KZ195574            12910 bp    DNA     linear   CON 06-SEP-2024
DEFINITION  Streptomyces sp. CS113 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ195574
VERSION     NZ_KZ195574.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS113
  ORGANISM  Streptomyces sp. CS113
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8695358)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8695358)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8695358)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 78.17x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188365.1-RS_2024_09_06
            Annotation Date                   :: 09/06/2024 02:31:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,790
            CDSs (total)                      :: 7,719
            Genes (coding)                    :: 7,614
            CDSs (with protein)               :: 7,614
            Genes (RNA)                       :: 71
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 35 of 105
            Pseudo Genes (incomplete)         :: 81 of 105
            Pseudo Genes (internal stop)      :: 4 of 105
            Pseudo Genes (multiple problems)  :: 15 of 105
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:56:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1728973
            Orig. end                         :: 1741883
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195574.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
FEATURES             Location/Qualifiers
     region          1..12910
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="10"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12910
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12910
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7910](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7910)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     gene            complement(409..2244)
                     /locus_tag="B9W62_RS07465"
                     /old_locus_tag="B9W62_07480"
     CDS             complement(409..2244)
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316421.1"
                     /locus_tag="B9W62_RS07465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_07480"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_087806367.1"
                     /transl_table=11
                     /translation="MGTTDTRGPAPGRSAVVLALRHYGRELLRLRRLALPALLLPAVGN
                     IGIRYVAPLLIAKLAGQAAEGDGLALGPALPYVLGFGVTLLLAEALWRIGQHCLNRVDA
                     LGMEHLYVSGMDELLAKDAAFFHDNFAGSLTKRVLSYGKRFEDFVDTVTYRIVGSLVPL
                     VFGAVVLWSYEPMLVVGLFVMIALTVAAATPLIRRRQRLVNEREAAIARVSGHVADSLM
                     NMETIRAFAAERREAGEHRSRVADSRRLTLKSWDYGNLRVDTLIAPMSVLTNVLGLLVA
                     LAFGGPGQGVEEVVVAFTYYSNATQIMFEFNQIYRRLESSMTEAAQFTELLLDPPTVLD
                     ADAPEPLAPRDTGIRFEAVTFAHTGAKPIFQGLDLDVPAGARIGLVGRSGGGKTTLTRL
                     LLRMSGLDRGRILIGGQDISRLRQSDLRSLIAYVPQEPAMFHRSLRDNIAFARPGATDE
                     EIHAAAEAAHVTEFAGQLPDGFGTLVGERGVKLSGGQRQRVALARALLRDAPVLLLDEA
                     TSALDSESELLVQDALWRLMDGRTALVVAHRLSTVAGMDRLVVLDRGRVVEQGSHQELL
                     ATDGAYARLWQHQSGGFLGENTGTESEAALERPVA"
     misc_feature    complement(2363..2379)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.35"
                     /tool="antismash"
     misc_feature    complement(2365..2375)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            2415..3128
                     /locus_tag="B9W62_RS07470"
                     /old_locus_tag="B9W62_07485"
     CDS             2415..3128
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019330889.1"
                     /locus_tag="B9W62_RS07470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_07485"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_087806369.1"
                     /transl_table=11
                     /translation="MPHLSSQAISVQTHLRDQVADALRAALIAGDLRPGVVYSAPTLAA
                     ELGVSATPVREAMLDLAREGLVEAVRNKGFRITEMTERDLDEFTEIRTLIEVPTVGRVT
                     ATATAGQLEALRPLAEQIVTAAREHDVLKYLEADHRFHLELLALAGNHHLVAVVADLRK
                     RSRLFGLGSLNETGRLVASAQEHVELLDLMVAGRTREAEECMRRHLTHVRTLWADGAAD
                     TGDAPGAAQSVKPAG"
     gene            complement(3104..4105)
                     /locus_tag="B9W62_RS07475"
                     /old_locus_tag="B9W62_07490"
     CDS             complement(3104..4105)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="B9W62_RS07475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_07490"
                     /product="proline racemase family protein"
                     /protein_id="WP_087806371.1"
                     /transl_table=11
                     /translation="MRSTVCYHAVDSHTEGMPTRVITGGVGVLPGATMFEKRQRFVAER
                     DHLRTLLMCEPRGHASMSGAILQPPTRPDADYGVLFIEVSGCLPMCGHGTIGVATVLVE
                     TGMVEVTEPETLVRLDTPAGLVTARVRVREGHAESVTLENVASYSHALDQVVDVPGYGE
                     VRYDIAYGGNFYAFVRTDDLGIPFERAHKQRLLDAGLAVMDAVNKQNPVSHPENPEIDV
                     CHHVYLEAPGSTAEHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGLLPAGRDFVNES
                     FIGSRFVGRVLGETTVGDRPAVLPSVTGRAWITGTAQYLLDPSDPYPAGFTL"
     gene            complement(4108..5004)
                     /locus_tag="B9W62_RS07480"
                     /old_locus_tag="B9W62_07495"
     CDS             complement(4108..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030799.1"
                     /locus_tag="B9W62_RS07480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_07495"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_087806373.1"
                     /transl_table=11
                     /translation="MSETRKPWHGVIVATGLPFHDDLSVDFAAYGESVAHLAEQGMHGV
                     APNGSLGEYQTLTYEERDRVVETAVANAPEGFTVMPGVGAYGGREAERHARFAKDAGCQ
                     AVMCLPPNAYRADDRAVLEHFERVASVGLPVTAYNNPIDTKVDLRPDLLAKLHAEGFVV
                     GVKEFSGDVRRCYEISELAPGLDLMIGTDDTVLEVALAGAKGWVAGYPQVFPRACLALY
                     EASVRGDLETALPLYRQLHPVLRWDSKTEFVQAIKLGQEMTGRRGGVCRPPRQPLAPET
                     EAVVRAATQALIDAGVN"
     gene            complement(5001..6500)
                     /locus_tag="B9W62_RS07485"
                     /old_locus_tag="B9W62_07500"
     CDS             complement(5001..6500)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030798.1"
                     /locus_tag="B9W62_RS07485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_07500"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_256976224.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 2e-17, bitscore:
                     62.2, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 1.1e-11, bitscore:
                     43.8, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDPADLIVVGAGPAGLAAAATALAGGLRVVLLDSGTALGGQYWR
                     HPPEHARAAVPTDDLHHGLDRYRALCKALAAHRTSGRLDLRLEHHVWSAVRDGDGFAVH
                     AVDRHEAPRETAVVLRAPRLLVATGAYDRPLPFPGWDLPGVLTVGGLQALLKGGSVAAG
                     SRVALGGTGPFLLPVAAGLAARGVEVVEVCEAAHPRAWLRHPGPLLRNPGKWAEGAGYA
                     AALVRHRVPLRPRTAIVAAEGGARVTAVRIASLAPDGSPRPGTGRRVEVDAVGVGWGFA
                     PQLDLLVPLGCALTEAADGNAAVAVDEGQRTTVPGLYTAGETCGVGGAALAVNEGRLAA
                     ASVLADSRATRVPANKHLTALRSAVERQRAFARAMALAHPLPPAWSSWLTDETVVCRCE
                     EVTAGTVRSAHADGAHDHRQVKQLTRAGMGWCQGRMCGPAVHCLTARRRAYEPAERLVA
                     TPVTLGALADLDEVPPTPQVPQALPTPQASTANGVTDKECS"
     gene            complement(6493..6747)
                     /locus_tag="B9W62_RS07490"
                     /old_locus_tag="B9W62_07505"
     CDS             complement(6493..6747)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972635.1"
                     /locus_tag="B9W62_RS07490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_07505"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_087806375.1"
                     /sec_met_domain="Fer2_4 (E-value: 8.4e-25, bitscore: 85.5,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEMIVDGRPLPFVEGQTVAAALVATGRLAWRTTRVGHRPRGVFC
                     GIGVCFDCLVTVDGAPGQRACLVPARPGMTVTTGEGDDD"
     gene            complement(6744..7910)
                     /locus_tag="B9W62_RS07495"
                     /old_locus_tag="B9W62_07510"
     CDS             complement(6744..7910)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Trp_halogenase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972636.1"
                     /locus_tag="B9W62_RS07495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_07510"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_087806377.1"
                     /sec_met_domain="DAO (E-value: 6.8e-68, bitscore: 228.6,
                     seeds: 521, tool: rule-based-clusters)"
                     /sec_met_domain="Trp_halogenase (E-value: 4.6e-05,
                     bitscore: 21.2, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRKVVVVGAGVVGAACAFHAASAGLDVTVTDRGPVGAGTTSRGE
                     GNVLLSDKEPGPELALARLSRTLWDEAGQELGPDTLELEPKGGLVVASGAENLATLREF
                     AARQEAAGVRVERVDRVRDLEPHLAPDIPGGVHYPQDAQVQPVLAAAALLRAALRRGAR
                     FHQGEVAGAVTGRDGRVTGVRTAAGDVLPADAVVNAAGTWGGEVGRRLGAPVEVLPRRG
                     FVLVTEPLPPMVRHKVYSADYVANVASSDAGLETSCVVEGTRGGTVLIGASRERVGFDT
                     TMNTAVVARLAAQACRLFPFLRGVHLMRAYRGFRPYCPDHLPVVGPDPRVPGVVHACGH
                     EGAGVGLAPGTGALVTAHLLGRPWRGADPAAHSGLLPDRFLTPGGVPQ"
     misc_feature    7940..7950
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: strong, score: 22.4"
                     /tool="antismash"
     gene            complement(8033..10228)
                     /locus_tag="B9W62_RS40635"
     CDS             complement(8033..10228)
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994025.1"
                     /locus_tag="B9W62_RS40635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SWIM zinc finger family protein"
                     /protein_id="WP_256976225.1"
                     /transl_table=11
                     /translation="MAREALRAARRQAKADAASEGGSANSAAPARRSSRPAGGQRPARG
                     PATADARARAVRDFAAGAFRLPPEEDPAEEALPAGESAGGPVAEVREVARRGSGSSPGG
                     DRAAARAVRGFAAGAFRLPPEEDPAEEALPAGESAGGPVAEVREVARRGSGSSPGGDRA
                     VRGSFAGAFRLPPEEDPAEEAPPARESAEGSATEGSATGSSASEGPAAAHPAPEPSPGA
                     DHSAGATGAERSAGFAADGGHVGGSADDGRTTGGALAPARAGHASRSEGAGSVAEWSRR
                     SQQQQQPAAARGRDGSVSAAQPRAPRSMAAPDRDGDLRRTFAALPPREAADEGFAGTWW
                     GNAWVTALEEGALDAARLERGRGYAERGHVDAITVTPGLVLGYVQGSRARPYRVQVRLR
                     TLGDADWDRFLDAAVERPGHIAALLDKELPHSLADLADRGVPLLPGPGDLTPQCSCPDS
                     GHPCKHAAALCYQTARLLDADPFVLLLLRGRGERALLDALSRRNAARAARAAQDRGPAP
                     LPGVRAGAALTPRALPPLPPPLPAPPHPEQPPAYPGAPGGPDPFALDQLATDAAARAHA
                     LLTTGRDPVGGLTLWQDAVRLAAARPGSGLTAGTRALYASLASAAGRDTAELARAVAAW
                     RQGGPEGLDVLEEPWDPPAGRFDRARPLLLAADLPAFRPWRNRLTHPRGHVQLRLGRAG
                     LWYAYESEPGREDWWPRGTPDLDPVGALTGLGTRVDL"
     misc_feature    10938..10952
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 20.47"
                     /tool="antismash"
     misc_feature    12044..12059
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 23.56"
                     /tool="antismash"
     misc_feature    complement(12251..12264)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.47"
                     /tool="antismash"
ORIGIN
        1 gacgggagcc cgacgccgcg aagatcgacg aggccgtggc cgccgcacgg ggccgggacg
       61 cggtggtcgt caccaccgac aacgtcggcc ccaccagtgc ccagcgcacc ctggtgtccc
      121 ggctggtcgc gacgggcgtc cccgtcgtcc acctcgcggt gcgcaacccc tacgacatcg
      181 cccacctcgg cgacgtcccg gcctcactgg cctcctactg ctggaccgag gtcgaactgc
      241 gcgccgccgc aagggtgatc gcgggccggg tcgatccgcg cggacgactg cccgtgcccg
      301 tccagcgggc cgacgacccg gcccgggtcc tcttcccgtc cgggcacggc ctgtcgtacg
      361 acgactgacg cggccgcccg ctccgtcacg tccgtgaccg cgggcccgtc aggcgaccgg
      421 gcgttccagg gccgcctccg actcggtccc ggtgttctcg ccgaggaagc cgccggactg
      481 gtgctgccag agtctggcgt aggcaccgtc cgtcgcgagc agttcctggt ggctgccctg
      541 ctcgacgacg cgcccgcggt cgaggacgac gagccggtcc atgccggcga cggtgctcag
      601 ccggtgggcg accaccaggg ccgtgcggcc gtccatcaac cgccacaggg cgtcctggac
      661 gaggagttcg ctctccgagt ccagcgcgct ggtcgcctca tcgagcagca gtaccggggc
      721 gtcgcgcagg agggccctgg cgagggcgac gcgctggcgc tgcccgccgg acagcttcac
      781 ccctctctcc ccgaccagcg tgccgaagcc gtcggggagt tgaccggcga actccgtgac
      841 gtgcgcggcc tcggcggccg cgtggatctc ctcgtcggtg gcgcccggcc gggcgaaggc
      901 gatgttgtcc cgcaggctgc ggtggaacat ggcgggttcc tgcgggacgt aggcgatcag
      961 cgagcgcagg tccgactggc gcagccggct gatgtcctga ccgccgatca ggatgcgtcc
     1021 gcggtcgagg ccggacatcc gcagcaggag ccgggtgagc gtggtcttgc cgccgcccga
     1081 cctgccgacc aggccgatcc gggcgcccgc gggcacgtcg aggtcgagtc cctggaagat
     1141 cggcttcgca ccggtgtggg cgaaggtcac cgcctcgaag cggatgccgg tgtcccgcgg
     1201 tgcgaggggt tcgggcgcgt cggcgtcgag caccgtgggc gggtccagca gcagctccgt
     1261 gaactgggcc gcttcggtca tcgagctttc cagccgccgg tagatctggt tgaactcgaa
     1321 catgatctgg gtggcgttgg agtagtaggt gaaggcgacg acgacctcct ccactccctg
     1381 accgggaccg ccgaaggcga gggcgaccag caggcccagc acgttggtca gtacggacat
     1441 gggcgcgatc agggtgtcga cgcgcaggtt gccgtagtcc caggacttca gggtcaggcg
     1501 ccgggagtcc gcgacccggc tgcggtgttc gccggcctcg cgccgttcgg ccgcgaaggc
     1561 ccggatggtc tccatgttca tgaggctgtc ggcgacatgg ccggagaccc gggcgatcgc
     1621 ggcctcccgt tcgttgacga gccgttgcct gcgccggatc agcggcgtcg cggccgccac
     1681 cgtcagcgcg atcatcacga acaggccgac gacgagcatc ggttcgtagc tccacagcac
     1741 cacggcaccg aagaccaggg gcacgagact gcccacgatc cggtacgtca cggtgtcgac
     1801 gaagtcctcg aagcgcttgc cgtagctgag gacccgcttg gtgagggagc cggcgaagtt
     1861 gtcgtggaag aacgcggcgt ccttggcgag gagttcgtcc atgccgctca cgtacaggtg
     1921 ctccatgccg agggcgtcca cccggttcag gcagtgctgc ccgatccgcc acagggcctc
     1981 ggcgagcagc agcgtcacac cgaagcccag cacgtacggc agagccgggc cgagggcgag
     2041 accgtcaccc tcggcggcct gcccggccaa cttggcgatc agcagggggg cgacgtagcg
     2101 gatgccgatg ttgcccacgg ccggcagcag cagcgcgggc agggccagtc gtcgcagtcg
     2161 cagcagttcc cggccgtagt ggcgcagcgc caggacgacc gcgctcctgc ccggcgccgg
     2221 ccctcgcgtg tctgttgtcc ccatcacact cccggaggtt cggaagcggg aagtctcccg
     2281 tcggacaggg gtcacggtcc aggcaattac cgcgcaccgt cgataaccgg acatcgtgga
     2341 gtcaacccgg acggcctacg caacgtgaca ttgtatagtg agggatcgtt ggctggacgg
     2401 acgcaggagc acggatgccg cacctctctt cacaggcgat ctccgtccag acccatctgc
     2461 gcgatcaggt cgccgacgcg ctgcgggccg ccctcatcgc cggcgacctg cgaccgggtg
     2521 tcgtctactc cgcccccacc ctcgccgcgg aactgggcgt ctccgccact ccggtgcgcg
     2581 aggccatgct ggacctggcc cgggaggggc tggtcgaggc ggtgcgcaac aagggtttcc
     2641 ggatcaccga gatgaccgag cgggacctcg acgagttcac cgagatccgg accctgatcg
     2701 aggttcccac cgtgggacgc gtcaccgcga cggcgaccgc cgggcagttg gaggcgctgc
     2761 gcccgctggc ggagcagatc gtcaccgcgg cccgggagca cgacgtcctc aagtacctcg
     2821 aggccgatca ccggttccat ctcgaactgc tggcgctggc cggcaaccac cacctcgtgg
     2881 cggtggtggc cgacctgcgc aagcgttcac gcctgttcgg tctgggctcc ctgaacgaga
     2941 ccgggcggct ggtcgcgtcc gcgcaggagc acgtcgagct gctggacctg atggtggccg
     3001 gccggacccg cgaggccgag gagtgcatgc gccgccatct cacccacgtc cgcacgctgt
     3061 gggcggacgg cgcggcggac accggcgacg cacccggggc ggctcagagc gtgaaacccg
     3121 ccgggtaggg gtcggagggg tcgagcaggt actgcgcggt gccggtgatc caggcgcggc
     3181 cggtgacgga cggcaggacg gccgggcggt cgcccaccgt cgtctccccc aggacccggc
     3241 cgacgaaccg ggagccgatg aaggactcgt tgacgaagtc ccgccccgcc gggagcagac
     3301 cgcgggcgtg cagctgcgcc atccgcgcgc tggtgccggt gccgcagggc gacctgtcga
     3361 accatcccgg atggatcgcc atggcgtgcc gtgagtgctc ggcggtcgat ccgggagcct
     3421 cgaggtagac gtggtggcac acgtcgatct ccggattctc cgggtgggac accgggttct
     3481 gcttgttgac cgcgtccatg acggccagcc cggcgtcgag cagccgctgc ttgtgcgccc
     3541 gctcgaaggg gatgccgagg tcgtcggtgc ggacgaaggc gtagaagttg ccgccgtagg
     3601 cgatgtcgta gcgcacttcc ccgtacccgg ggacgtcgac gacctggtcg agtgcgtggc
     3661 tgtacgaggc gacgttctcc agggtcaccg actccgcgtg gccctcgcgc acccgcaccc
     3721 gggccgtgac cagtcccgcc ggggtgtcga gccgtaccag ggtctccggc tcggtcacct
     3781 cgaccatgcc ggtctcgacg aggaccgtcg cgaccccgat ggtgccgtgc ccgcacatcg
     3841 gcaggcagcc ggagacctcg atgaacagga cgccgtagtc ggcgtcgggc cgggtgggcg
     3901 gctggaggat ggccccggac atcgaggcgt ggccgcgcgg ttcgcacatg agcagggtgc
     3961 gcaggtggtc gcgttcggcg acgaaccgct gccgcttctc gaacatcgtc gcgccgggca
     4021 ggacgcccac cccgcccgtg atcacgcggg tgggcatgcc ctcggtgtgc gagtcgacgg
     4081 cgtggtagca gaccgtcgag cgcatcgtca gttcaccccc gcgtcgatca gggcctgggt
     4141 ggcggcgcgc acgacggcct ccgtctcggg agccagcggc tgtcgcggcg ggcggcagac
     4201 accaccgcgg cggccggtca tctcctggcc gagcttgatg gcctggacga actccgtctt
     4261 gctgtcccag cgcagcaccg ggtgcagttg gcggtagagc ggcagcgccg tctccagatc
     4321 gccccggacg gacgcctcgt acagcgcgag acaggcccgg gggaagacct gcgggtaccc
     4381 ggcgacccag cccttggcgc cggcgagggc gacctcgagg acggtgtcgt cggtgccgat
     4441 catgaggtcg aggccggggg ccagctcgga gatctcgtag cagcgccgga cgtcgccgga
     4501 gaactccttg accccgacga cgaacccctc ggcgtggagc ttcgccagca ggtcggggcg
     4561 caggtccacc ttggtgtcga tggggttgtt gtacgcggtg acgggcaggc ccaccgacgc
     4621 cacccgctcg aagtgttcga gcaccgcgcg gtcgtcggcg cggtaggcgt tgggcggcag
     4681 gcacatgacg gcctggcagc ccgcgtcctt ggcgaacctg gcgtgccgct cggcctcccg
     4741 gccgccgtac gcgccgacgc ccggcatcac ggtgaacccc tcgggtgcgt tcgcgacggc
     4801 ggtctcgacg acgcggtcgc gctcctcgta cgtgagggtc tggtactcgc ccagcgaccc
     4861 gttcggtgcg acgccgtgca tcccctgctc ggcgaggtgg gcgacgctct cgccgtaggc
     4921 cgcgaagtcg accgagaggt cgtcgtggaa cggcaggccg gtcgcgacga tgacgccgtg
     4981 ccagggcttg cgggtctcgc tcatgaacac tccttgtcgg tcacaccgtt ggccgtcgac
     5041 gcctgcggtg tcggcagtgc ctgcggtact tgaggtgtcg gcggtacttc gtcgaggtcg
     5101 gcgagcgccc cgagggtgac gggcgtcgcg acgagccgtt ccgcgggctc gtaggcccgc
     5161 cgccgtgccg tgaggcagtg cacggcgggc ccgcacatcc ggccctggca ccagcccatc
     5221 ccggcccggg tcagctgctt gacctgccgg tggtcgtgtg cgccgtcggc gtgggcgctg
     5281 cggaccgtcc cggcggtcac ctcctcacag cggcacacga ccgtctcgtc ggtcagccac
     5341 gaggaccagg cgggaggaag cggatgggcc agggccatgg cccgggcgaa ggcacgctgt
     5401 cgctccaccg ccgaacgcag ggcggtcagg tgcttgttcg ccggtacgcg agtggcgcgc
     5461 gagtcggcga ggacggacgc cgcggccagc cggccctcgt tcacggcgag cgccgccccg
     5521 cccactccac acgtctcgcc cgcggtgtag aggccgggga cggtggtgcg ctggccctcg
     5581 tcgaccgcga ccgccgcgtt gccgtcggcc gcctcggtga gggcgcagcc gagggggacg
     5641 agcaggtcga gctggggcgc gaaaccccag ccgacgccga cggcgtcgac ctcgacgcgt
     5701 cgtcccgtgc ccggccgcgg gctgccgtcc ggcgccagcg aggcgatccg taccgcggtc
     5761 acgcgtgcgc cgccctccgc ggcgacgatc gccgtgcgcg ggcgcagggg gacgcggtgg
     5821 cgtacgagtg cggcggcgta cccggcgccc tcggcccact tgccggggtt gcgcagcagc
     5881 gggccggggt gtcgcagcca cgcccgcggg tgggccgcct cgcacacctc gacgacctcg
     5941 acgccgcgcg ccgcgagacc ggcggcgacc ggcagcagga acgggccggt gccgcccagc
     6001 gccacgcggc ttccggccgc cactgagccg cccttgagca gcgcctgtag tccgccgacg
     6061 gtcagcaccc cgggaaggtc ccagcccggg aagggcagtg ggcggtcgta ggcgccggtg
     6121 gcgaccagga gtctcggcgc ccgcaggacg acggcggtct cccgcggtgc ctcgtggcgg
     6181 tcgacggcgt ggacggcgaa cccgtcgccg tcacggacgg cggaccacac atggtgttcc
     6241 aggcgcaggt cgagccggcc cgacgtccgg tgtgcggcca gcgccttgca cagggcgcgg
     6301 tagcggtcca gtccgtggtg caggtcgtcg gtcggtacgg ccgctcgggc gtgctcgggc
     6361 gggtggcgcc agtactgacc gccgagggcg gttcccgagt ccagcagtac gacgcgcagt
     6421 ccaccggcca gcgcggtggc ggcggcggcc aggccggccg ggcccgcgcc gacgacgatc
     6481 aggtccgcgg ggtcagtcat cgtcgccctc cccggtcgtg acggtcatac ccggccgggc
     6541 cggcaccagg caggcgcgct gcccgggggc tccgtcgacg gtgacgaggc agtcgaagca
     6601 gacgccgatg ccgcagaaga cgccacgcgg gcggtgcccg acgcgggtgg tgcgccaggc
     6661 gaggcggccc gtggccacga gggccgcggc gacggtctgt ccctcgacga acggcagggg
     6721 ccggccgtcg acgatcatct cggtcactgg ggcacacctc cgggggtgag gaaacggtcc
     6781 gggagaaggc cgctgtgggc ggccgggtcg gcaccgcgcc agggccggcc gagcaggtgg
     6841 gcggtgacga gcgcgcccgt tccgggggcc agtccgacgc ccgctccctc gtgcccgcac
     6901 gcgtggacga cgcccggcac gcgcgggtcg gggcccacca ccggcaggtg gtccgggcag
     6961 tacggccgga acccgcggta ggcgcgcatc agatggaccc cgcgcaggaa ggggaagagc
     7021 cggcacgcct gggccgcgag cctggcgacc acggcggtgt tcatcgtggt gtcgaagccg
     7081 acgcgttccc ggctggcgcc gatgaggacg gtaccgccgc gggtgccctc cacgacgcac
     7141 gaagtctcca gtccggcgtc ggaggaggcg acgttggcca cgtagtcggc ggagtagacc
     7201 ttgtgccgga ccatcggcgg cagcggttcg gtcaccagga cgaagccgcg gcgcggcagc
     7261 acctcgacgg gagcgccgag ccgtcggccg acctcaccgc cccaggtccc ggcggcgttg
     7321 acgacggcgt cggcgggcag cacgtcacct gcggccgtcc ggacaccggt gacgcggccg
     7381 tcccggccgg tcaccgcccc ggccacctcg ccctggtgga agcgcgcgcc gcgccgcagg
     7441 gcggctcgca gcagcgcggc cgccgccagc accggctgca cctgtgcgtc ctgcgggtag
     7501 tggacaccgc cggggatgtc gggtgcgagg tggggttcga ggtcccgcac ccggtcgacc
     7561 cgctcgacgc ggacgccggc cgcctcctgc cgggcggcga actcccggag cgtcgcgagg
     7621 ttctcggcgc cgctcgcgac gaccaggccg cccttgggct cgagttcgag ggtgtcgggg
     7681 cccagctcct ggccggcctc gtcccacagg gtgcggctca ggcgggccag ggccagttcg
     7741 gggccgggtt ccttgtcgga gaggaggacg ttgccctctc cgcggctcgt cgtcccggcg
     7801 ccgacggggc cgcggtcggt gaccgtgacg tccaggcccg ccgaggcggc gtggaaggcg
     7861 caggcggctc cgacgactcc cgcgcccacg acgacgactt tccgggtcaa cggctcctcc
     7921 tcgcgcacgc atcccactgt gcaatgtcac atgccatggg tgaggctata catccggcag
     7981 gcgtaagaca acgcccacgt cgccgacttc gcgcccgggc ggtcggggag ggtcagaggt
     8041 cgactcgggt gccgaggccg gtcagggcgc cgaccgggtc gaggtcgggt gtgccgcgcg
     8101 gccaccagtc ctcgcggccg ggctccgact cgtacgcgta ccagagcccc gcgcggccga
     8161 gtctgagctg gacatggccc cgggggtggg tgaggcggtt gcgccagggg cggaaggcgg
     8221 ggagatcggc ggcgagcagc agggggcggg cgcggtcgaa ccggccggcc ggcgggtccc
     8281 agggctcctc caggacgtcg aggccctcgg gtccgccctg ccgccaggcg gcgacggccc
     8341 gcgccagctc cgccgtgtcg cgcccggcgg ccgaggcgag ggacgcgtag agcgcgcggg
     8401 tgcccgcggt gagcccggag ccggggcgcg ccgcggcgag ccggacggcg tcctgccaca
     8461 gcgtcagtcc gccgaccggg tcccgtccgg tggtgagcag ggcgtgcgcg cgggcggccg
     8521 cgtcggtggc caactggtcc agcgcgaacg ggtcggggcc gcccggtgcg cccgggtagg
     8581 caggcggctg ctcggggtgc gggggcgcgg gcagcggcgg cgggaggggc ggcagggcgc
     8641 gcggggtcag ggccgcaccg gcccggacgc cgggcagggg cgccggtccg cggtcctggg
     8701 ccgcacgggc cgcgcgggcc gcgttccgcc gggacagggc gtcgagcagc gcgcgctcgc
     8761 cccggccgcg cagcaggagc aggacgaagg ggtcggcgtc gagcagacgt gccgtctggt
     8821 agcagagggc ggcggcgtgc ttgcaggggt gaccggagtc ggggcagctg cactgcgggg
     8881 tgaggtcgcc ggggccgggg agcagcggga cgccgcggtc ggccaaatcg gccagggagt
     8941 gcggcagttc cttgtcgagc agggcggcga tgtgcccggg ccgttcgacg gcggcgtcca
     9001 ggaaccggtc ccagtcggcg tcgccgagcg tccgcagccg cacctggacg cggtacggcc
     9061 gggcgcggct gccctgcacg tagccgagga ccagccccgg cgtcacggtg atggcgtcga
     9121 cgtgtccccg ctccgcgtag ccgcgcccgc gctccagccg ggcggcgtcc agggcgcctt
     9181 cctccagcgc cgtcacccac gcgtttcccc accaggtccc ggcgaagccc tcgtctgcgg
     9241 cctcccgggg cggcaacgcg gcaaaggtgc ggcggaggtc gccgtcgcgg tccggggcag
     9301 ccatcgaacg aggtgcgcgg ggctgcgcgg ccgacacgga gccgtcccgg ccgcgcgccg
     9361 ccgccggctg ctgctgctgc tgcgaacgcc gcgaccactc ggcgacggac cccgcccctt
     9421 ccgaacgcga cgcgtgcccg gcccgtgcgg gggcgagggc gcccccggtg gtacgaccgt
     9481 cgtcggcgga tccgccgaca tgaccgccgt cggcggcgaa tccggcactg cgttcggcac
     9541 cggtggctcc ggcactgtgg tcggcgccgg gcgacggctc cggcgccggg tgggcggccg
     9601 ccgggccctc cgaggcactg cttccggtgg cggagccctc ggtggcggaa ccctcggccg
     9661 actcccgggc aggcggggcc tcctcggccg ggtcctcctc cgggggcagc cggaaggcgc
     9721 ccgcgaagga accccgtacg gcacggtcgc ccccgggcga cgaacccgac ccccggcgcg
     9781 cgacctcccg cacctcggcg accggcccac cggccgactc cccagcgggc agggcctcct
     9841 cggccgggtc ctcctccggg ggcagccgga aggcgcccgc cgcaaagccc cgtaccgcac
     9901 gagccgcggc acggtcgccc ccgggcgacg aacccgaccc ccggcgcgcg acctcccgca
     9961 cctcggcgac cggcccaccg gccgactccc cagcgggcag ggcctcctcg gccgggtcct
    10021 cctccggggg cagccggaag gcgcccgcgg cgaagtcgcg tacggcacgg gcgcgggcgt
    10081 cggcggtggc cgggccgcgc gctggccgct gaccaccggc cgggcgggac gaacgccgcg
    10141 cgggtgccgc gctgttggcg gacccgcctt cggacgccgc gtccgccttc gcctgccgac
    10201 gcgccgcccg caacgcctcg cgcgccacgt ccgccggacg ggcggaccgc gcgccgactg
    10261 agccggggga cgacggcccg tcgggaacac ctcgaccgcc cgaaccgtcc aagccgtccg
    10321 aaccgtccga gctgtccggt tcggtcgtgt cgtcggtccc ggcggtcttc tcgccgggaa
    10381 ctgtcggctc cccgccgggc tgagggtcct ccgagcccgt cgggtcggct cggtctggcg
    10441 aggcgaccgg ggcgaccggt tccgccggga ctccgcgatc tcccagggcc gccgaaccgg
    10501 ccgggagtgg acccggtggc ggcgtgtcgg aggcgttggc ggcgtcgggc tcgccggggg
    10561 cgtcgtgtgg cgtgtcaggg acgtctgcgg ggtcgtccgc ccccgtatgc cgtgcggtgc
    10621 gcgcgctacg cagtgcggcg cgggccatgt ccccggggcg tgacggggtg gggtcccccg
    10681 gcgtctgctc cgagaccccc tcccgccccg cggccggatc cgcggccggg gccggcgacg
    10741 acggcgacga cgaccggggc ggtgacggcg ccccgcggtc gccgtcaccg ctcgtcggct
    10801 cgcctcgccc ccgtgcctcc cgcagggcgc ggcgggcctc gtcggcgggc cggggggtca
    10861 cgacggcctc cgcagggaca cgaggtcgga cagctcacgg gccgtcagtt cggtgagggc
    10921 cgactccccg gagccgagga tcgcgtcggc cagggcccgc ttggactgga gcatctcggc
    10981 gatgcggtcc tcgaccgtgc cctcggtgat gagccggtgg acctggaccg gctgcgtctg
    11041 tccgatccgg tacgcccggt cggtggcctg ctcctccacc gcagggttcc accagcggtc
    11101 gaagtgcacg acgtgaccgg cacgggtgag gttgaggccg gtgccggcgg ccttcaggga
    11161 caggacgagg acgggggtcg cgccgctctg gaagcggtcc accatccgct cgcgctccgg
    11221 caccggcgtg cccccgtgca gcaggtcgac cgggaccgcc cgggcggtca ggtgcgcggt
    11281 gatgaggcgg gccatgccga cgtactgcgt gaagaccagc gccgagccgt cctccgcgag
    11341 caccgtgtcc aacagctcgt ccagcagcgc cagtttgccc gagcgggcgg tcatccgttc
    11401 ggcgccgccc ggcgggtgct cctccttcag gaacagcgcg ggatggtcgc agatctgctt
    11461 cagcgaggtc agcagcttga gcacgagccc gcggcggccc atgccctcgg cctcctcgat
    11521 ggcgagcatc gactcgcgca ccaccgcctc gtacagcgcg gcctgctcgc gggtgagcgg
    11581 gaccgggtgg tccgtctcgg tcttcggcgg cagctccggg acgatgcccg ggtcggactt
    11641 cctgcggcgc agcaggaagg ggcggatcag ccgggcgagg cgctccaccg cctggtcgtc
    11701 ctcgccgttc tccaccgcgc gcgcgtgccg ggcgcggaag gatttcaggg ggccgagcag
    11761 tccgggcgtg gtccagtcga gcagggccca cagctcggag aggttgttct ccacgggggt
    11821 gccggtcagg gccacccgcg ccggggacgg gatggtgcgc agggccttcg ccgtcgccga
    11881 gtacgggttc ttgacgtgct gcgcctcgtc cgcgacgacc atgccccagg gctgctcggc
    11941 cagggtcgtg gcggcggacc gcatggtgcc gtaggtggtg aggacgaagc cgccgctcag
    12001 gtcgtcgagg gtgcggtcgg ctccgtggaa gcggcggacc gggacgccgg gcgcgaaccg
    12061 ctcgatctcc cgctgccagt tgcccagcag ggaggccggg cagaccacca gggtcggttc
    12121 ggtgcgggcc cgtttcaggt gcagggcgat gacggtgacg gtcttgccga ggcccatgtc
    12181 gtcggcgagg cagccgccaa ggccgagcga ggtcatgagg tggagccagg cgaggccgcg
    12241 cagctggtag tcgcgcaggg tggcgtgcag gccgggcggc ggttcggcgg ggcgcacgcc
    12301 tgccgtcagc cggtcgcgca gggcggccag cgcgccgacg ggcaccacgt cgaccgtctc
    12361 gccgtcggcc tccgcgctgc cggtgagggc gacggacagg gcgtcgaccg ggtcgagcag
    12421 gcccagctcg cgcttgcggg cccggcggac gagcgccggg tcgaccagca cccagcggtc
    12481 ccggagccgg acgacgggac ggtgggcctc cgcgagggtg tccatctcgg cctcggtgag
    12541 cgggtcgccg ccgatcgcga gctgccagcg gaactggagg aggtcctcgc tctcgaagaa
    12601 ccccgtgccg tcggtcgccg aaccgggcgc cgtcctgatc accgcggtcg ccgtgaggtc
    12661 ctgggccagg tcccgcggcc agtgcacggc gaccccggcg gcggcgagcc gcccggccgc
    12721 caccccgagc aggtcggtga cctcctcctc ggacagggcg agcacgtcgg gcacgtcctg
    12781 ctcggtgagc cggtccagcg gcggccagac ccgggccgcg cgccgtaccg ccagggcggt
    12841 gtccacgcgc gcgcgggccc cgaacgccgc gtcggccgtc cccgaccaca ggtccgccgc
    12901 gtcggcgacg
//
