LOCUS       NZ_KZ195574            10216 bp    DNA     linear   CON 06-SEP-2024
DEFINITION  Streptomyces sp. CS113 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ195574
VERSION     NZ_KZ195574.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS113
  ORGANISM  Streptomyces sp. CS113
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8695358)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8695358)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8695358)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 78.17x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188365.1-RS_2024_09_06
            Annotation Date                   :: 09/06/2024 02:31:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,790
            CDSs (total)                      :: 7,719
            Genes (coding)                    :: 7,614
            CDSs (with protein)               :: 7,614
            Genes (RNA)                       :: 71
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 35 of 105
            Pseudo Genes (incomplete)         :: 81 of 105
            Pseudo Genes (internal stop)      :: 4 of 105
            Pseudo Genes (multiple problems)  :: 15 of 105
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:56:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 719342
            Orig. end                         :: 729558
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195574.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5216
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(220..2715)
                     /locus_tag="B9W62_RS03000"
                     /old_locus_tag="B9W62_03010"
     CDS             complement(220..2715)
                     /EC_number="3.1.3.16"
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978110.1"
                     /locus_tag="B9W62_RS03000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_03010"
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_087804918.1"
                     /transl_table=11
                     /translation="MSYKRCNGDVVSQGAAERGPRTLRAEGALKAIAAGPQPEQRLRGI
                     LEQALVFAGASLAALYAPGDDPELLCLVESAGVPRTLYGVRDSYPASGGSPVADAHRAG
                     EAVWMGPEEFAGSAESRHVPSSGFSLAVLPVRGDGGGCLLALTERPDGFDADDRACLEV
                     IAEAMTCPVPAETVETAEHVEGGALRPDAFTLAMDTGRAEVGDDILRLFGLAPGEFDGK
                     VETLLGLTVPEDLPSLMSVTESDHMSIGERELEFRVLQPTGPPKWLRLSGRLLPGGDGR
                     PTLLVGTVADASTLRPDVTDVARVQQLAAALATAGTVRDVSQAVVAALRRPLRADRIAL
                     AELENDRLVVTVLDPPDPESWPELWRLEWRTEWPDAPVRAMPTLAAALREGRARIWPAG
                     TDDVEPALADVGPGGLAVLPLPAGGRMAGACLIGWDTPHDFGPDERALLTASAGLAGQA
                     LMRAHAFDAEHELVGMLQRQLLPRRLPKLPGAVAVARYLPSTAGLELGGDWYDVIPLPD
                     HHVALVIGDVQGHSAGAATLMGQMRTALRAYAVEVHPPDVVVSHANRLLTGMETDLFAT
                     CVYVDLDMEEGSAWCVRAGHLPPVLRHPDGSTEIAEVEGGPPLGVVTQADFPMSPLRLR
                     PGTLIALTTDGLVETPESDIDEGMDRFAHALAVSAPAHLGLVADALLGNARRNDDVALL
                     LVRYDGMALRPLRESWSVWRLPEAVRHARRFTRRTLRIWDIPDDDIDGVLLIVSELVTN
                     ALVHTDGKVRLDLTLISGRLRVAVADASPRTPVKPTSIGWEATGGRGILLVEAMSATWG
                     TLPVSGGKQVWSEIPVGGG"
     misc_feature    complement(2696..2709)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.56"
                     /tool="antismash"
     gene            complement(3120..4418)
                     /locus_tag="B9W62_RS03005"
                     /old_locus_tag="B9W62_03015"
     CDS             complement(3120..4418)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978109.1"
                     /locus_tag="B9W62_RS03005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_03015"
                     /product="S1 family peptidase"
                     /protein_id="WP_087804919.1"
                     /transl_table=11
                     /translation="MSHKRIPKRRAAMAAGGVVALGAAAILLPNANASQDGGSPDDAPA
                     APRTLKAADASDLAVRLAGLLGDTFAGSYYDAAARQLVVNVVPGGDDQVVTQAEKAGAE
                     VREVDHSMGELRSGAQTLKSEASIPGTSWAIDPRTNKILVTADRTVTGDRWDRLESTVE
                     GLGSGMATVRKSAGTFKTFASGGDAIFGGGARCSLGFNVTAGDGSPAFLTAGHCGVAAD
                     QWSDAQGGQPVGTVDQAVFPGEGDFALVTYDDPATEAPSEVNLGDQTLPISGPAEATVG
                     QEVFRMGSTTGLADGQVLGLDATVNYPEGTVTGLIQTDVCAEPGDSGGSLFTRDGLAIG
                     LTSGGSGDCTVGGETFFQPVTTALEAVGATLGGEDAGAGAGEEGAGGAGDVGDAGDGSG
                     EGSGVGSEDGSGVGETGDGAAVGGGDGTDGLTG"
     gene            5001..5216
                     /locus_tag="B9W62_RS03010"
                     /old_locus_tag="B9W62_03020"
     CDS             5001..5216
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="B9W62_RS03010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_03020"
                     /product="hypothetical protein"
                     /protein_id="WP_003978108.1"
                     /sec_met_domain="strepbact (E-value: 2.3e-37, bitscore:
                     126.1, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTPQVETVEISDADLDNVSGGLNVNAVGTVTGLVDGIAPVSGL
                     LNTAVGTVEGVTGLNTAPVTGLVAGL"
     gene            5337..6143
                     /locus_tag="B9W62_RS03015"
                     /old_locus_tag="B9W62_03025"
     CDS             5337..6143
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="B9W62_RS03015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_03025"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_087804921.1"
                     /transl_table=11
                     /translation="MQFRQQALAKLQSPEELDLPVRFARPQGWLVLSVTVVAMAAASVW
                     AVGGSVTSTVAAPAILTHGQGSYLLQSPVSGQVTAVLARPGQRLAADAPVLKVRTAEGE
                     TVVRSIDAGRVSALAATVGQIISTGANVASVEKVAHADDPLYATVYVPAEKAAAIPAHA
                     SVDLTVQSVPTQQYGVLHGEVKAVDRSAQSAQTITAFLGDSQLGEQFTEDGRPVAVTVR
                     LDTSESTKSGYAWSSADGPPFGLTSMTLASGSIRLADQRPVDWLLP"
     gene            6140..8362
                     /locus_tag="B9W62_RS03020"
                     /old_locus_tag="B9W62_03030"
     CDS             6140..8362
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978106.1"
                     /locus_tag="B9W62_RS03020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_03030"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_087804923.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.9e-39, bitscore:
                     133.3, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTAQETRGRRRAAPPKRTVPKPRAKTVRTPTVLQMEAVECGAAS
                     LAMVLGHYGRHVPLEELRIACGVSRDGSRASNLLKAARSYGFTAKGMQMDLAALAEVTA
                     PAILFWEFNHYVVYDGMGRRFGRRGVFINDPGKGRRFVSLEDFDGSFTGVVLTMEPGED
                     FTRGGRRPGVLGAMPARLRGTAGTLPAAVLASLLLVAVGAAVPALSRTYIDMFLIGGQT
                     SLLGVLFASMGACVLLTVVLTWLQQANLLHGRIISSTLSSARFLRHLLRLPVTFFSQRS
                     PADLVQRLQSNDAVAETLARDLAAAGVDAIVVVLYAVLLYTYDPQLTFVGIGVALLNIL
                     AMRVVIRLRATRTAKLRADTARLTNTAYTGLQLIETMKATGGEDGYFRKWAGQHATTLE
                     EQQRLGVPSAWLGVVAPTLATLNSGLILWIGGMRAVEGHISVGLLVAFQALVVRFTAPL
                     TRLNGVAGRIQDFAADVARLKDVENFRADPLYDRPGGGDSTRRLHGHVELQNISFGYNP
                     LDKPLLTGFDLTVGPGQQVALVGGSGSGKSTVSRLISGLYAPWDGVIRIDGQRLDDIPR
                     GALASSVSFVDQDVFLFEGSVRDNVALWDPSIPDDAVVDALKDAALYDVVMRRPGGLHS
                     PVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALDAETELVVMDNLRRRGCACV
                     VIAHRLSTVRDSDEIVVLEHGTVVERGRHAELVAHGGAYAALVGER"
     misc_feature    complement(9036..9050)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.56"
                     /tool="antismash"
     misc_feature    9261..9277
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.67"
                     /tool="antismash"
     misc_feature    complement(9692..9701)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
ORIGIN
        1 cggacggcgg gtttgccgcc ggtgtccgtg gtcacggggc atcatcttcc ttctcgttct
       61 gcatcggtgc gctcgccatt ccgggtcccc gggcggggca ccgcgaaaca ccgggcacat
      121 gtgtcccgcg gtgcccaggt gttggggccg ggccgcgctg atgtcgtggg gcagccgggc
      181 gggtacccgg cggtgcggtg gccgaaccgg ccggggcgtt cagcctccgc ccaccgggat
      241 ctcgctccac acctgcttgc ccccgctgac cggcagcgtc ccccaggtcg ccgacatcgc
      301 ctcgacgagg aggatgccgc gcccgcccgt ggcctcccag ccgatgctgg tcggtttgac
      361 gggtgtacgc ggcgaggcgt cggcgaccgc gacgcgcagc cggccgctga tcagggtgag
      421 gtcgagccgt accttgccgt cggtgtgcac cagggcgttg gtgaccagct ccgagacgat
      481 cagcaggacg ccgtcgatgt cgtcgtccgg tatgtcccag atgcgcaggg tgcgccgggt
      541 gaagcggcgg gcgtgccgca cggcctccgg cagccgccac accgaccagc tctcgcgcag
      601 cgggcgcagg gccatgccgt cgtagcgcac cagcagcagg gcgacgtcgt cgttgcgccg
      661 ggcgttgccg agcagggcgt cggcgacgag tccgaggtgg gcgggggcgg agacggccag
      721 ggcgtgtgcg aaccggtcca tgccctcgtc gatgtcggac tccggcgtct ccaccaggcc
      781 gtccgtggtg agggcgatca gcgtgccggg ccgcagccgc agcgggctca tcgggaagtc
      841 ggcctgcgtg accacgccga gcggcgggcc gccctcgacc tccgcgatct ccgtgctgcc
      901 gtcggggtgg cgcagcaccg gcggcaggtg cccggcccgc acgcaccagg cggagccctc
      961 ctccatgtcg aggtcgacgt agacgcaggt ggcaaagagg tcggtctcca tacccgtcag
     1021 cagccggttg gcgtgggaca ccaccacgtc cggcgggtgg acctcgacgg cgtaggcgcg
     1081 cagggcggtg cgcatctggc ccatcagggt ggccgcgccg gcgctgtggc cctggacgtc
     1141 gccgatgacc agggccacgt ggtggtcggg cagcgggatc acgtcgtacc agtcgccgcc
     1201 cagctcgagg cccgccgtgc tgggcaggta gcgggcgacg gcgaccgcgc cgggcagctt
     1261 cggcagccgg cgcggcagca gctggcgctg gagcatgccg accagttcgt gctcggcgtc
     1321 gaaggcgtgg gcgcgcatca gggcctggcc ggcgagaccg gcggaggcgg tgagcagggc
     1381 gcgctcgtcg gggccgaagt cgtgcggcgt gtcccagccg atcaggcagg cgccggccat
     1441 gcggccgccg gcgggcagcg gcagcacggc caggccgccg gggccgacgt cggcgagggc
     1501 cggttcgacg tcgtcggtgc cggcgggcca gatccgggcg cggccctcgc gcagcgcggc
     1561 ggcgagggtg ggcatggccc gtaccggtgc gtcgggccac tcggtgcgcc actccaggcg
     1621 ccacagctcg ggccaggact cggggtcggg cgggtcgagg acggtgacga cgagccggtc
     1681 gttctccagc tcggccagcg cgatgcggtc ggccctgagc ggtctgcgca gggcggcgac
     1741 gacggcctgg ctgacgtcgc ggacggtccc ggcggtggcg agcgcggcgg cgagctgctg
     1801 cacgcgggcg acatcggtca cgtcggggcg cagcgtggag gcgtcggcga cggtgccgac
     1861 caggagggtc ggcctgccgt cgccgccggg cagcaggcgc ccgctgagcc gcagccactt
     1921 cggcggaccg gtgggctgta gcacacggaa ctccagctcg cgctcgccga tgctcatgtg
     1981 gtcggactcg gtcaccgaca tcagggacgg caggtcctcc ggcacggtga ggccgagcag
     2041 cgtctcgacc ttgccgtcga actccccggg ggcgaggccg aacaggcgca ggatgtcgtc
     2101 accgacctcc gcccggccgg tgtccatggc cagggtgaag gcgtccggcc ggagcgcacc
     2161 gccctcgacg tgctcggcgg tctccacggt ctcggccggg accgggcagg tcatcgcctc
     2221 ggcgatcacc tcgagacacg cacggtcgtc ggcgtcgaac ccgtccgggc gctcggtcag
     2281 ggcgaggagg cagccgccgc cgtctccgcg cacgggcagg acggcgagcg agaagccgct
     2341 cgacggcacg tgccgcgact ccgccgatcc ggcgaactcc tccggcccca tccacaccgc
     2401 ctcgccggcc cggtgggcgt cggcgaccgg ggagccgccg gaggcgggat agctgtcgcg
     2461 tacgccgtac agggtgcggg gcactccggc cgactcgacc aggcagagca gctcggggtc
     2521 gtcgccgggc gcgtagaggg cggccaagga ggcgcccgcg aagaccaggg cctgctcgag
     2581 gatgccccgc aggcgctgct ccggctgcgg gccggccgcg atcgccttca gggcgccctc
     2641 ggcacgcagc gtcctcggtc cgcgttccgc agcgccctga ctgaccacgt cgccattaca
     2701 gcgcttatag gacatctgcg cagcccctac ggctcgggta ccggcgatca ggagcacttc
     2761 gggggctcgc ggaccggcat tgcgcgccgc ggtgcggcgg gacgcactcg tggggcgggg
     2821 gcgggcacgg tcgggcaggg gcgggtacgg cacgcggcgc ggcgccgcgc gaggtaccgc
     2881 gaggcggtcc cgggcccggt ggccactggt gcgcgagggc gcggggcgcg ggtgccgggt
     2941 gcgcgggtgc gcgcgaggga ggggcacgga ccgtcgggcc cgcccggggg ctcccgccgc
     3001 ccgggccttc ggccgccgga tcgccgcccg gtcccgtcgg cgggggtccg ggcggcgatc
     3061 cgtgggccgt ggtgtcaacc cgtggtgtca gcccatcagc ccatcagccc atcagcccgt
     3121 cagcccgtca gcccgtcggt cccgtccccg ccgccgaccg ccgcaccgtc gccggtctca
     3181 ccgacaccgg acccgtcttc agagccgaca ccggaccctt caccggagcc gtcaccggca
     3241 tcgccgacgt ctcctgcacc acctgccccc tcctccccgg cacccgcgcc ggcgtcctcg
     3301 ccgccgaggg tggcgccgac cgcctccagg gccgtggtga ccggctggaa gaaggtctcg
     3361 ccgccgaccg tgcagtcacc gctgccaccc gaggtcaggc cgatcgcgag gccgtcccgg
     3421 gtgaacaggg agccgccgct gtcgcccggc tcggcgcaca cgtcggtctg tatgaggccg
     3481 gtgacggtgc cctcggggta gttgacggtg gcgtccaggc cgaggacctg gccgtcggcc
     3541 agcccggtcg tactgcccat gcgaaagacc tcctggccga ccgtcgcctc ggcgggcccg
     3601 ctgatcggca gggtctggtc gccgaggttc acctcgctcg gcgcctcggt cgccgggtcg
     3661 tcgtaagtga cgagtgcgaa gtcgccctcg ccggggaaga ccgcctggtc gacggtgccg
     3721 accggctgcc cgccctgcgc gtcggaccac tggtcggccg cgaccccgca gtgaccggcc
     3781 gtcaggaagg cgggcgagcc gtcgcccgcg gtgacgttga agccgagcga gcagcgcgca
     3841 ccgccgccga agatggcgtc gccgcccgac gcgaaggtct tgaaggtgcc ggcggacttc
     3901 ctgaccgtgg ccatgccgga gccgaggccc tcgacggtgg actccagccg gtcccaacgg
     3961 tcgccggtga cggtgcggtc cgcggtcacc aggatcttgt tggtgcgcgg gtcgatcgcc
     4021 cacgaggtgc ccgggatgct cgcctcggac ttcagggtct gcgcgccgct cctcagctcg
     4081 cccatgctgt ggtcgacctc gcgcacctcc gcgcccgcct tctcggcctg cgtgacgacc
     4141 tggtcgtcgc cgccggggac gacgttgacg accagttgcc gcgcggcggc gtcgtagtag
     4201 gagcccgcga aggtgtcgcc gagcagcccg gcgagccgta cggccagatc cgacgcgtcc
     4261 gccgccttga gggtcctggg cgcggccggg gcgtcgtcgg gggagccgcc gtcctgcgag
     4321 gcgttggcgt tcggcagcag gatcgcggcc gcgccgagcg ccaccacacc gcccgccgcc
     4381 atcgcggccc tgcgcttcgg gattcgcttg tgactcaacc tgctcgacct cctgggaccg
     4441 gggttggcac ccgccggtcc ggcagctgtt ggggggcgcc cggacggctg ttggtacgca
     4501 cgggccgccc cggacgttca gccggacccc cgccccgccc ccggccggca ctctccgcgt
     4561 ccgatcggcg acgcaccgtt ccgatcacgg agcggaatga ccgcttcagt gaatctgcac
     4621 atcgaatgcc caacccggtc actcccgttg gcgcatctgc acaagccgga ggcggcccgt
     4681 acgtctttgg ggccggagtg tcccaatcgc ctccccggag gcaaggggtc gcacgagtcg
     4741 atgtcgcgca gcgtgtgaag aagtcgccgc gaaggcgtgc gcgctcctgc acgcttgggt
     4801 gcccgggcgt cgcaagtgcg ctggtgagag gggtgattga ttggaagccc ggcccggtcg
     4861 cgtgctttga tccatcgcac cgcgggggcc gcggagggct cgggaaacgg gcgcccggcg
     4921 cccgcggtcg cggccgtcat ctgtccccag agggggccgc tccccccttg tgaaatggct
     4981 atacgaaggg aagttccatc atgaactcca ccccccaggt tgagaccgtc gagatctccg
     5041 acgccgacct cgacaacgtc tccggcggcc tgaacgtgaa cgccgtcggc accgtcaccg
     5101 gcctggtgga cggcatcgcc ccggtctccg gcctgctcaa cacggccgtc ggcaccgtcg
     5161 agggtgtcac cggcctgaac accgccccgg tcacgggcct ggtcgccggt ctctgatcgc
     5221 gcccttgcgt cgctgagtcc cggagccgtc caggctccgg gacttttcgg ccgttcccga
     5281 cttcccgacg ttctcgtcat gtcggccgtt cacaccgagc cctggattcg tcagccgtgc
     5341 agttccgcca acaggccctc gccaaactcc agtcaccgga ggagctcgat ctcccggtgc
     5401 gcttcgcccg tccccagggc tggctggtgc tgtccgtgac cgtggtcgcg atggccgccg
     5461 cgtccgtgtg ggcggtgggc ggctcggtga cctccacggt cgccgctccc gccatcctca
     5521 cccacgggca gggcagttac ctgttgcaga gtcccgtctc ggggcaggtc accgcggttc
     5581 tggcccgccc ggggcagcgg ctggccgccg acgccccggt gctgaaggtc cgcaccgccg
     5641 agggcgagac ggtggtccgc tcgatcgacg ccggccgggt cagcgcgctc gcggccaccg
     5701 tcgggcagat catcagcacc ggcgcgaacg tcgcctcggt cgagaaggtc gcccacgccg
     5761 acgaccccct gtacgccacc gtctacgtcc ccgccgagaa ggccgccgcc atccccgcgc
     5821 acgcctccgt cgacctgacc gtccagtcgg tgccgacaca gcagtacggc gtcctgcacg
     5881 gcgaggtgaa ggccgtggac cgctcggccc agtcggcgca gacgatcacc gcgttcctcg
     5941 gggacagcca gctcggcgag cagttcaccg aggacggcag gccggtggcc gtgacggtgc
     6001 ggctggacac ctccgagtcc acgaagagcg gctacgcgtg gtcctcggcc gacggaccgc
     6061 cgttcggact gacctccatg acgctggcct cgggctccat ccggctcgcc gaccagcgtc
     6121 ccgtcgattg gctgctgccg tgagcaccgc acaggagacc cggggcaggc gccgggccgc
     6181 cccgcccaag cgcacggtgc ccaagccgcg ggcgaagacg gtccgcacac ccaccgtgct
     6241 ccagatggag gccgtggagt gcggtgccgc ctccctggcg atggtcctcg gccactacgg
     6301 ccgccacgtc ccgctggagg agctgcgcat cgcctgcggc gtctcccgcg acggctcgcg
     6361 cgccagcaat ctgctgaagg cggcccggag ttacgggttc accgccaagg gcatgcagat
     6421 ggacctggcc gccctcgccg aggtgacggc gcccgccatc ctgttctggg agttcaacca
     6481 ctacgtcgtc tacgacggga tgggccgccg gttcgggcgg cgcggggttt tcatcaacga
     6541 cccgggcaag ggccgccgtt tcgtgtccct ggaggacttc gacggcagct tcaccggggt
     6601 cgtgctgacc atggagcccg gcgaggactt cacccggggc ggccgccggc cgggtgtgct
     6661 cggcgcgatg ccggcccggc tgcgcggcac ggccgggacg ctgcccgccg ccgtgctggc
     6721 gagtctgctc ctggtggcgg tcggcgcggc ggtgcccgcg ctcagccgca cctacatcga
     6781 catgttcctg atcggggggc agacctcgct gctcggcgtg ctgttcgcgt cgatgggggc
     6841 gtgcgtcctg ctcacggtgg tgctgacctg gctccagcag gccaacctgc tgcacgggcg
     6901 catcatctcc tccaccctgt ccagcgcccg cttcctgcgg cacctgctgc ggctgccggt
     6961 gaccttcttc tcccagcgca gcccggccga cctggtgcag cggctccagt ccaacgacgc
     7021 cgtcgccgag acgctggccc gcgacctcgc cgcggcgggc gtggacgcga tcgtggtcgt
     7081 cctgtacgcc gtgctgctgt acacgtacga cccgcagctc accttcgtcg gcatcggcgt
     7141 ggcgctgctc aacatcctgg cgatgcgggt cgtgatccgg ctgcgcgcga cccgtacggc
     7201 gaagctgcgg gcggacacgg cgcggctgac caacaccgcg tacaccggcc tccagctgat
     7261 cgagacgatg aaggcgaccg gcggcgagga cggctacttc cgcaagtggg ccggccagca
     7321 cgccaccacg ctggaggagc agcagcgact cggggtgccg agcgcctggc tgggggtggt
     7381 ggcacccacg ctggcgacgc tcaacagcgg gctgatcctg tggatcggcg gcatgcgcgc
     7441 ggtcgagggc cacatctcgg tcggtctgct ggtcgccttc caggcgctgg tggtccgctt
     7501 cacggccccg ctgacccggc tgaacggcgt cgcgggccgc atccaggact tcgcggccga
     7561 cgtggcccgg ctgaaggacg tggagaactt ccgcgcggac ccgctctacg accgccccgg
     7621 cggcggcgac tcgacccgcc ggctgcacgg ccacgtcgag ttgcagaaca tctccttcgg
     7681 ctacaacccg ctcgacaaac cgctgctcac cggcttcgac ctgaccgtcg gcccggggca
     7741 gcaggtggcc ctggtcggcg ggtcgggcag cggcaagtcg acggtgtcgc ggctgatctc
     7801 gggcctgtac gcgccctggg acggcgtgat ccgcatcgac gggcagcggc tcgacgacat
     7861 cccgcgcggg gcgctggcgt cctccgtctc cttcgtcgac caggacgtgt tcctgttcga
     7921 gggctcggtg cgcgacaacg tggcgctgtg ggacccgtcg atccccgacg acgccgtggt
     7981 ggacgcgctc aaggacgccg cgctgtacga cgtggtgatg cgcaggccgg gcggcctcca
     8041 cagcccggtc gagcaggacg gccggaactt ctccggcggg cagcgccagc gcctggagat
     8101 cgcgcgggcc ctggtgcgcc ggccgagcat cctcgtcctg gacgaggtga ccagcgcgct
     8161 ggacgcggag acggagctgg tggtcatgga caacctgcgc cggcgcggct gcgcctgcgt
     8221 ggtgatcgcg caccggctca gcaccgtgcg ggacagcgac gagatcgtcg tcctggagca
     8281 cggcacggtg gtggagcgcg ggcggcacgc ggagctggtg gcacacggtg gcgcgtacgc
     8341 ggcgctggtc ggggagcgat gagatgacga ccgtgcagga gggacagggc gacctcgtcc
     8401 tcggggcgct cggctcgctg ggcacccgcg tcgactgcgc cgggttcaac cgcctcgacc
     8461 tggaggggcc gcaggtgctg tggctggtgg tgtccggtgc ggtggacctg ttcgcggtcg
     8521 acgccgggga gcagggccac tggcaccacc tgggacgcct ggaggcgggc tcggcgctcc
     8581 tcgggccggt cgccggaccg cggcacacgc tggtggcgcg tccgctgcgg gactgcgtgg
     8641 tgcaccgcat cggactgcgc gagctgtacc agccggcgaa cacccagacc tggtcgtacg
     8701 acgcgtacgg caacccgcag tacgtgccgc cgacgacgag cccgctggag tacgccctgg
     8761 ccctcggcgt ggggcgtggc ctgacggtcc tcttccaggc gccgatggcc gcggagcggg
     8821 ccggcgcacc cacggacgac gacgtgttct ggatgcaggt gccgccgggc agcgtgcagt
     8881 acggggcggt gtacggcgag gaggcggccg ccgacctgct gatggacccg gcgctgtggc
     8941 agagcctggt cgaccagcaa taccggctgc tgaccactct ggaccggtgg atcgagcagc
     9001 tggagcgtac gcacgagacg cgcacggcgg ccggtatcga ggccggtgag gcggtgcgcg
     9061 cgcaggccga ccggacgctg ctggcctcca tcggcaagcg ctcggcgcag cgcgccacgg
     9121 ccgcggacgc ggacgccacg tacgcagcgt gcaggctggt cgcccgcgcc gccgggatcc
     9181 cgctggccga accggccctg ggcggcaccg agagcgaccg gctcgacccg gtggagcgca
     9241 tcgcgctcgc ctctcgggtg cgcaccaggt cggtgcggct ggcggaccgc tggtggcggg
     9301 aggacgtggg gccgctggtc gggcaccgca ccctgtccgg ggcgccggtg gcgctgctgt
     9361 ggcggcgcgg cggctatgcg gccgtgcatc cggcgaccgg tcgtgagacg ccggtggagc
     9421 gggcgaacgc cggggagttc gagccgcgcg cggtgatgtt ctaccggccg ctgccggagc
     9481 ggcggctcag tccgctgcga ctgctgcggt tctgtctggc gggcacgcgc cgggatctga
     9541 ccggtctgct gctcgccggt ctggtgacgg tggcgctcgg cgcgctggtg ccgatcgcga
     9601 cgggccgggt gctcggcgag ttcgtgccga aggcgcagac cgggctgatc gtgcaggtgt
     9661 gtctcgcggt gatgctgagc agtgtcgtcg cggccgcctt catgctgctc cagaacctca
     9721 ccatcctgcg tctggagggc cggatcgagg cgacgctcca gcccgccgtg tgggaccggc
     9781 tgctgcggct gccgacgaag ttcttcaccg agcgctccac cggcgagctg gcgagcgcgg
     9841 ccatgggcat cagcgcgatc cgcaggctcc tcgcgggcgt cggtccgacg gtcgcccagt
     9901 cggtgacggt cggggcgatg aacctgggcc tgctgctctg gtacagcgtg ccgatggcgc
     9961 tcgcggcgat cggcatgctg gtggtggtcg ccggggtctt cctggggctc gggctgtggc
    10021 aggtgcgctg gcagcggcgg ctggtcaagc tcaccaacaa gctgaacaac caggcgttcc
    10081 agacgctgcg cggactgccc aagctgcggg tggcggcggc cgagaactac gcctacgcgg
    10141 cgtgggcgga gcggttcgcg cacagccggg agttgcagca gcgggcgggg cggatcaaga
    10201 acctgaacgc ggtgct
//
