LOCUS       NZ_KZ195586             6753 bp    DNA     linear   CON 07-SEP-2024
DEFINITION  Streptomyces sp. CS227 scaffold00004, whole genome shotgun sequence.
ACCESSION   NZ_KZ195586
VERSION     NZ_KZ195586.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS227
  ORGANISM  Streptomyces sp. CS227
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 24091)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24091)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 24091)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 370.61x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188355.1-RS_2024_09_07
            Annotation Date                   :: 09/07/2024 02:15:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,017
            CDSs (total)                      :: 5,954
            Genes (coding)                    :: 5,785
            CDSs (with protein)               :: 5,785
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 72 of 169
            Pseudo Genes (incomplete)         :: 127 of 169
            Pseudo Genes (internal stop)      :: 12 of 169
            Pseudo Genes (multiple problems)  :: 41 of 169
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:26:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6753
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195586.1.
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..6753
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..6753
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6753
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[748:1753](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      749..1753
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(241..432)
                     /locus_tag="B9W68_RS32615"
                     /old_locus_tag="B9W68_30415"
     CDS             complement(241..432)
                     /GO_function="GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA]"
                     /GO_function="GO:0004658 - propionyl-CoA carboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025192.5"
                     /locus_tag="B9W68_RS32615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_30415"
                     /product="acyl-CoA carboxylase epsilon subunit"
                     /protein_id="WP_087778057.1"
                     /transl_table=11
                     /translation="MESREAVFRVERGQADAAELAAVAAVLLAVSRNRGPVVSRRRARA
                     GSSWWRSAGGYRSPASWR"
     misc_feature    complement(407..421)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.64"
                     /tool="antismash"
     misc_feature    685..700
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.79"
                     /tool="antismash"
     gene            749..1753
                     /locus_tag="B9W68_RS30085"
                     /old_locus_tag="B9W68_30420"
     CDS             749..1753
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406753.1"
                     /locus_tag="B9W68_RS30085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_30420"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_087778058.1"
                     /sec_met_domain="AfsA (E-value: 6.4e-25, bitscore: 77.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTSTFRGHHATAGPTEPPTTEETPQRPARPGSSHHPALTTTVPK
                     EFVHRAGVAEVMLTDWARTDDTHFTLAAQWPRGHSFFTAVEGCHDPFVAAETIRQAGAL
                     LAHAEFGVPLGHNFLMWELGFAVNPERMRVDGAPASLELEIACFDVKRRGTGLSGMRYE
                     AVIRRDGHVAATGGATFTCTSPRVYQRLRAERLALGRPQPPLTAPTAPQTVGRVSPMDV
                     VLSPTGKQGRWQLRVDTRHPVLFEHPVDHVPGMMLLEAARQATAATLGSPTLPLRVTST
                     FARYAELDAPCLIEARRLPGDGTAREESVLVTAVQEGELTFSATVTAASPRRD"
     gene            complement(1813..2667)
                     /locus_tag="B9W68_RS30090"
                     /old_locus_tag="B9W68_30425"
     CDS             complement(1813..2667)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019331212.1"
                     /locus_tag="B9W68_RS30090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_30425"
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_087778059.1"
                     /transl_table=11
                     /translation="MILVTGATGTVGREVVRLLHAAPERSRVRVMTRDPARLTGACRAV
                     EAVAADYRDPSSLAGALAGVRTAFLVTTGVGGDDDAAFLRAARSAGVRRVVKLSAAAVT
                     DPGADDAITTWQRGTEELLRASGMEWTLLRPRSFLSNTLSWAPSVRSGRVVRALYGTSR
                     NAGVDPRDVAAAAALALTGDGHAGQAYTLTGPSAISAAGQTEQLGRELGVPLRLEELTR
                     EQARAAWSRRHPDTVVEALLLSAERQRAGAKARVDPAFRALTGRDPGSFAAWVRDHRGA
                     FAG"
     misc_feature    2688..2704
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.9"
                     /tool="antismash"
     misc_feature    2691..2707
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.25"
                     /tool="antismash"
     misc_feature    2706..2721
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.86"
                     /tool="antismash"
     misc_feature    2711..2720
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.21"
                     /tool="antismash"
     gene            2757..3425
                     /locus_tag="B9W68_RS30095"
                     /old_locus_tag="B9W68_30430"
     CDS             2757..3425
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387458.1"
                     /locus_tag="B9W68_RS30095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_30430"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_087778060.1"
                     /transl_table=11
                     /translation="MVKQERAIQTRKTILSAAARVFEGRGYQAATISEILTAAGVTKGA
                     LYFHFPSKEHLAQGVLHEQDQWLPVPERACKVQELVDTVMLHAYRLQTDPMVRAGVRLS
                     LDQQAHGLDRSGPFTRWAEILRSLLEESQAQGELLPNVVPVETADVLVGAFAGTQAMSQ
                     AVCEYQDLLRRSSHLLRHLLPSVAVPAVLASLDSSEARGERISTELRTLMSQQAEPAAG
                     "
     gene            complement(3446..5017)
                     /locus_tag="B9W68_RS30100"
                     /old_locus_tag="B9W68_30435"
     CDS             complement(3446..5017)
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106290.1"
                     /locus_tag="B9W68_RS30100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_30435"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_256968768.1"
                     /transl_table=11
                     /translation="MRAQQHVRVRAQVIVVGGGPVGMFLACELAGHGVRTLVVEAATAV
                     SQRPRATTLHARTVQCLARRGHLDALLPDGSGTGAPVSRSPFHFAGIPGLVVTAPVTEP
                     EPIVKCAQERLERHFERRAKAAGARVLRGHRVTRVHQGQDGFTVTAEAPRGRVLECVAD
                     YVVGADGARSTVREQAGIGSRTYPATVSAMAGDVRLDDAADLRPGWHRTPRGWLVVKDV
                     PDGSVRLRTVNCSGPHRARHLPLTLEELSREVGWIAGREIAMSSPRWLSRFSDFSRLAD
                     TYRSGRVLLAGDAAHVHFPIGGQGLSTGLLDAVGLGWKLAYTVLGRAGEGLLDSYDQER
                     RPEAQRVIDHARAQLALMRPDPELDPLRALFGELLTGVRGTDGTGGGESSYLGAMVSAQ
                     GTALPARTEGASSWEGTFLRNVSLTTPGGRTDVIGLLRAGRPLLLRFGERGRKYGGQGR
                     DWRYGVVRVVDADPTPELPCDALLVRPDGYVAWAPGGEALATALSVYFGEGGAVAPRGA
                     RVPVGG"
     gene            complement(5163..5588)
                     /locus_tag="B9W68_RS30105"
                     /old_locus_tag="B9W68_30440"
     CDS             complement(5163..5588)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106289.1"
                     /locus_tag="B9W68_RS30105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_30440"
                     /product="DUF6009 family protein"
                     /protein_id="WP_087778062.1"
                     /transl_table=11
                     /translation="MSALITEDEISQEATLVWLENVDELDYVRQSLDRLPTRRGKPAYH
                     RDGRMIGYAQLGSRAKASRSSGTFRRRVFWLLPHDRDTDPDGLYATGAPSEAVDPRTLA
                     PRVKGRMTERSRGDACQEGGPRAQELAREATPGSAPG"
     misc_feature    5962..5976
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
ORIGIN
        1 gccggggccc gccagcgccc cggcgcggac accgtggagc tcggccacgc gcccgcgcag
       61 gtcgcccggc cccggctcgg tggatgcagc gacggtcgtc atcgccatgt cagcccttcc
      121 taggacgagg gacgagggac gagggctgga ccgagggtcc gccccgggcc cgcacagccg
      181 atagtaacaa accgcatgcg cggtatatag agttgccccc caagggctta acaccctcgc
      241 tcaacgccag ctggcggggg accggtagcc acccgccgac ctccaccagg acgagccggc
      301 ccgggctcgc cgacggctca ccacaggtcc gcggttccgg ctcaccgcga gcaacacggc
      361 ggcgaccgcc gccagttccg cggcgtcagc ctgcccgcgc tccacccgga acaccgcttc
      421 ccgactctcc atccggccgg ttcccccttc gccaggactc agggacacgg tcgaccggcc
      481 cgctcaagaa ccgctacgga accgctggag tttcggccca gcgaggacca gttacggacc
      541 ggcccgcgcg ccggtccgtg gcgccgacaa cgttgccctc cgccggaaaa cccaagacca
      601 gcaaaagtcc gcaggtcagg cgtggacgcc gagcgggccg cgcacacgaa cgaaatgctt
      661 cgttccgcta gaaactaaac cggatggtat gtacgttcga ccggaagaac gccatccccg
      721 ttcttcacga cgcggctcag ggggacccat gtctacgagc acgttccgag gccatcacgc
      781 gacggccggc ccgactgagc caccaacgac ggaggagacg ccgcaacggc ccgcccggcc
      841 cggctcctcg caccacccgg ccctgacgac caccgttccc aaggagttcg tccaccgcgc
      901 cggggtcgcg gaggtcatgc tcaccgactg ggcacggacc gacgacaccc acttcaccct
      961 cgccgcccag tggccgcgcg ggcacagctt cttcaccgcc gtcgagggct gtcacgaccc
     1021 cttcgtggcg gccgagacga tccgccaggc cggcgccctc ctcgcacacg ccgagttcgg
     1081 tgtccccctc ggccacaact tcctcatgtg ggagctcggg ttcgcggtca acccggagcg
     1141 gatgcgcgtc gacggcgccc ccgcctcact cgaactggag atcgcctgct tcgacgtcaa
     1201 acggcggggc accggcctgt cgggcatgcg gtacgaggcg gtgatccgcc gggacggcca
     1261 cgtcgccgcc accggagggg ccacgttcac ctgcacctcg ccccgggtgt accagcgcct
     1321 gcgcgccgaa cgcctcgccc tcggcaggcc gcagcccccg ctcaccgccc cgacggcacc
     1381 gcagaccgtc ggccgggtct cccccatgga cgtcgtcctc tcccccaccg ggaaacaagg
     1441 ccggtggcag ctccgcgtgg acacccggca cccggtgctc ttcgagcacc ccgtcgacca
     1501 cgtgccgggc atgatgctcc tcgaagcggc ccgccaggcc acggccgcca ccctcggctc
     1561 ccccaccctc cccctccgcg tcaccagcac cttcgcgcgc tacgcggaac tcgacgcccc
     1621 ctgcctgatc gaggcccggc gcctgcccgg cgacggcacg gcccgggagg aatccgtcct
     1681 cgtgaccgcg gtccaggagg gcgaactcac cttcagcgcc acggtcaccg cggcgtcccc
     1741 gcgcagggac tgacgcaccg ctcacccgcg taccggcctc ctgggcaccg ctgccaggag
     1801 gcctccgacg cgtcagcccg cgaacgcacc ccggtgatcc ctcacccatg ccgcgaagga
     1861 ccccgggtcc cgcccggtca gcgcgcggaa cgccgggtcg acccgggcct tggcccccgc
     1921 ccgctgccgc tccgcgctca gcagcagcgc ctcgacgacc gtgtccgggt gacgccggct
     1981 ccaggccgcc cgggcctgct cccgggtcag ctcctccagc cgcagcggca ccccgagctc
     2041 ccgcccgagc tgctccgtct gcccggccgc gctgatcgcc gagggcccgg tcagggtgta
     2101 cgcctgtccc gcgtgcccgt ccccggtcag ggccagtgcg gcggcggccg ccacgtcgcg
     2161 ggggtccaca ccggcgttgc gcgacgtccc gtacagggcg cggacgaccc ggccggagcg
     2221 gacggacggc gcccaggaca aggtgttgga gaggaaggag cgcggccgga gcagcgtcca
     2281 ctccatgccg gacgcgcgca gcagttcctc ggtgccgcgc tgccaggtgg tgatggcgtc
     2341 gtcggctccg gggtcggtca cggcagccgc ggagagcttc accacccgcc gcacaccggc
     2401 cgaacgggcc gcgcggagga aggcggcgtc gtcgtcaccg ccgacaccgg tcgtgacgag
     2461 gaacgccgtc cgcaccccgg ccaacgcccc cgccagcgag gacgggtcgc ggtagtccgc
     2521 ggccaccgcc tccaccgccc ggcaggcgcc cgtcagccgg gcgggatcgc gcgtcatgac
     2581 ccggacccgc gagcgctccg gagctgcgtg gagcagccgg acgacctccc ggcccacggt
     2641 ccctgtcgca ccggtcacca ggatcatcgc gtctctccct gttaaactcg ctgtaatata
     2701 cgaactggac ggttttttgc cgggtccccg ggctcactga aaagtaggag gcctgcatgg
     2761 tgaagcagga acgggcgatc cagacccgga agacgatcct ctcggccgcc gccagggtct
     2821 tcgagggccg gggctaccag gccgcgacca tcagcgagat cctcaccgcc gcaggggtga
     2881 ccaagggagc cctgtacttc catttcccgt ccaaggagca cctggcccag ggcgtcctgc
     2941 acgagcagga ccagtggctg cccgtccccg agcgtgcctg caaggtccag gaactcgtcg
     3001 acacggtcat gctgcacgcc taccgcctcc agaccgaccc gatggtccgg gccggcgtcc
     3061 ggctctcgct cgaccagcag gcccacggcc tcgaccggag cgggccgttc acacgctggg
     3121 ccgagatact ccgcagcctg ctcgaagagt cccaggccca gggcgagttg ctgccgaacg
     3181 tcgtgccggt cgagaccgcc gacgtcctgg tcggcgcctt cgccggcacc caggccatgt
     3241 cccaggccgt gtgcgagtac caggacctgc tgcgccgcag ctcccacctg ctgcgccacc
     3301 tgctgcccag cgtcgcggtc cccgccgtgc tcgcctccct ggactcctcc gaggcccgtg
     3361 gcgagaggat ctccaccgag ctgcgcacac tgatgtccca gcaggccgag ccggcagccg
     3421 gctgacgacc gccggcaacg ccggatcacc ccccgaccgg gacgcgggca cctcgggggg
     3481 ccacggcgcc gccctcgccg aagtagaccg acagggcggt ggccagcgct tccccgcccg
     3541 gcgcccacgc gacgtagccg tcgggccgta cgagcaacgc gtcgcagggc agttcgggcg
     3601 tggggtcggc gtccacgacg cgcacgaccc cgtaccgcca gtcccggccc tgtcccccgt
     3661 acttccggcc ccgttcgccg aaacgcagca gcagcgggcg gcccgcgcgc agcagtccga
     3721 tcacatcggt ccgtccgccg ggcgtcgtca ggctgacgtt gcgcaggaac gtgccctccc
     3781 aggacgaggc gccttcggtg cgcgccggca gcgccgtgcc ctgggcactg accatggcac
     3841 cgaggtagga actctccccg ccccccgtac cgtcggtgcc ccggactccg gtcagcagct
     3901 cgccgaacag cgcccgcagc gggtcgagtt ccgggtcggg ccgcatcagg gcgagctggg
     3961 cccgcgcgtg gtcgatgact cgctgcgcct cgggtcttcg ctcctggtcg taactgtcga
     4021 gcagcccctc ccccgccctg ccgagcaccg tgtacgccag cttccagccc agccccaccg
     4081 cgtccagcag cccggtgctg agcccctgcc cgccgatcgg gaagtgcacg tgcgccgcgt
     4141 ccccggccag caggacacgc cccgagcggt aggtgtccgc gagccgggag aagtcgctga
     4201 acctgctcag ccaccggggc gagctcatgg cgatctcccg cccggcgatc cagccgacct
     4261 cgcggctcag ctcctccagc gtgagcggca ggtgccgtgc acggtgcggg cccgaacagt
     4321 tgacggtccg caggcggacg ctcccgtcgg ggacgtcctt gacgacgagc cagccgcgcg
     4381 gcgtgcggtg ccaccccggc cgcaggtcgg cggcgtcgtc cagccggacg tctcccgcca
     4441 tcgcggagac ggtcgccggg tacgtccggg agccgatccc ggcctgctcg cgcaccgtgc
     4501 tgcgggcgcc atcggccccg accacgtagt cggcgacgca ctccaggacg cgcccgcgcg
     4561 gggcctccgc ggtgacggtg aagccgtcct ggccctggtg cacccgggtc acgcggtggc
     4621 ctcgcagaac gcgtgcgccg gcggccttcg cgcgccgctc gaagtgcctc tccagccgct
     4681 cctgcgcgca cttgacgatg ggctcgggct cggtgacggg agccgtgacg accagcccgg
     4741 gaatcccggc gaaatggaag gggctgcggc tcaccggggc cccggtcccg ctcccgtccg
     4801 ggaggagcgc gtcgaggtgc ccgcgccggg ccagacactg aaccgtccgg gcgtgcaggg
     4861 tggtcgccct gggccgctgt gacacggcgg tcgccgcctc gacgaccagg gtccgtaccc
     4921 cgtgcccggc cagttcgcag gcgaggaaca tcccgaccgg gccgcccccc acgacgatca
     4981 cctgggctcg tacccgcaca tgctgctgcg cccgcacggc gattcccccc tcggccggcc
     5041 cacgctcacg cgtggcaccg tccagctcgc gcatctacgc gagaaaatat accgggtgga
     5101 cggttctttg caatgtcgtg accggccttc gccgaacggg gcgcgtgacc gacctcgcca
     5161 cgtcagccgg gggccgaccc gggcgtggcc tcccgggcca gctcctgggc ccgtgggccg
     5221 ccttcctggc acgcgtctcc tctggaccgc tcggtcatcc gccctttgac ccggggcgcc
     5281 agcgtgcgcg ggtccacggc ctcggacggc gccccggtcg cgtacaggcc gtcggggtcg
     5341 gtgtcgcggt cgtgcggcag cagccagaag acgcggcggc ggaaggtgcc cgacgaccgg
     5401 gacgccttgg cccgcgaccc cagctgggcg tagccgatca tccgcccgtc ccggtggtag
     5461 gcgggctttc cccgacgggt cggcagccgg tccaggctct gccgtacgta gtcgagttcg
     5521 tcgacgttct ccagccacac cagggtggct tcctggctga tctcgtcctc ggtgatgagg
     5581 gcactcatac ggtctcccca tgggcgagca gcccgatgcc cgggtagtac ttccgctggt
     5641 tggagaggat catccccttc ggggactcca gccccaccgc ctcccgcacc cgtgccgcga
     5701 acgaccggga cgagaggggc aaagcgccct cattgtggca ccacaccctg tactcctggt
     5761 agagctgcgt ctgttcggct ctcaggtccg cctccagccg gcaggactcg gcgaggaagc
     5821 ggcccgtgtg gtcctcggtc tccgcgtagg cggcggtcgc gacccgcacc cgctcggggc
     5881 cgatgaggtc cttgccgccg cccaggtagc ggcgggagcc gtcgatgagc cagttcagga
     5941 tgcccggccc ctcctcggtg acgaggaggt cggccaggtt gtcgatcttg cggtcgtcgg
     6001 ggacgacccg ctcgaagggg atcaggcgca tccggcgcca gaaggcgaag ccgccggtgc
     6061 cgacctcggg ccggtggttg cccagcagcc acagcttgtg ggtcggctcg aagctgaaga
     6121 agtcctgccg catccgtctc gccttgatcc ggtccccgcc ggtcaggagc ttgacccgcg
     6181 cctcgtcgaa gcggtcgccc ggcttcacct cggagcagac gacgacccgg cggccgtgca
     6241 gctcggcgag gtccgtgggg tgcccttcgt agggccgggc catcaggaag ccgggcggcg
     6301 cggcgtcggc gtagtcgccg aggagcttga gcaggacgtc cagcaggacg gacttgccgt
     6361 tcttgccgga gccgaagagg aagggcatga cctggccgcc gacgtccccc gtgatggagt
     6421 accccagcag cacctggagg aagtcgatca tctcctcgcc ctcggcctgc gcgccgaagg
     6481 tgtcggtgag gaaccgcagc cagcgcggcg tgggcatccg gcgggggctg acggcggtgg
     6541 agcgggagtg gaagtcctgc tcgggggagg cggagcggac cgcgccggtc cgcaggtcga
     6601 cgatgccgcc cggggtgcac agggcgtacg ggtcggcgtc gagcagggcc gcgttgagga
     6661 ccatgcccgg ggccgatctc gcctgtacga gcatcgagtt catgccggag gtgctcaacg
     6721 cgcggcggcg gtgctgctgg agggcctggg cgg
//
