LOCUS       NZ_KZ195583            10399 bp    DNA     linear   CON 07-SEP-2024
DEFINITION  Streptomyces sp. CS227 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ195583
VERSION     NZ_KZ195583.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS227
  ORGANISM  Streptomyces sp. CS227
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6794013)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794013)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6794013)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 370.61x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188355.1-RS_2024_09_07
            Annotation Date                   :: 09/07/2024 02:15:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,017
            CDSs (total)                      :: 5,954
            Genes (coding)                    :: 5,785
            CDSs (with protein)               :: 5,785
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 72 of 169
            Pseudo Genes (incomplete)         :: 127 of 169
            Pseudo Genes (internal stop)      :: 12 of 169
            Pseudo Genes (multiple problems)  :: 41 of 169
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:26:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5602913
            Orig. end                         :: 5613312
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195583.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="19"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            270..995
                     /locus_tag="B9W68_RS24850"
                     /old_locus_tag="B9W68_24900"
     CDS             270..995
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412186.1"
                     /locus_tag="B9W68_RS24850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_24900"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_087776591.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFDKALAAFPHRDRIEVVHRSFELDP
                     ARPKGDPELVLPMLAKKYGMSEAQAAEAERNLGQNAAAEGLPYLTEGRDHGSTFDMHRL
                     LHLAKARGRQEELLAVLYRANFAETETVFGVDERLVVLAVEAGLEEAEAREVLADEQRY
                     AAEVRADERAATELGANGVPFFVLDRRYGVSGAQPADVFTQALTQAWESHVPASGLATP
                     AGAEGGEVCGPDGCEVPKG"
     gene            1125..2174
                     /locus_tag="B9W68_RS24855"
                     /old_locus_tag="B9W68_24905"
     CDS             1125..2174
                     /NRPS_PKS="Domain: Aminotran_5 (55-343). E-value: 6.3e-24.
                     Score: 76.5. Matches aSDomain:
                     nrpspksdomains_B9W68_RS24855_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018895195.1"
                     /locus_tag="B9W68_RS24855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_24905"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_087776593.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.3e-18, bitscore:
                     64.7, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLARSLAEDEFKPSLTHLATASTGLLPLSAARAAHEAVDAAAAG
                     APPDMFGARDAARASFARLVGVPATRVATGSSVATFTGLIAAALPDGAEVLTADGDFSS
                     LVNPFHVRPGLTVRTVPLERIAEHVTEATALVAVSAVQSADGRRADLTAVREAARAHGA
                     RTLVDASQAAGWDTFEAGAYDYTVAVGFKWLLAPKGTAFLTVPEEPGALSPLLAGWVAG
                     DSPFDSCYGPVTDLAPDARRYDESPAPLVWAGAQHALALLERIGTEAIGAHDVALADRF
                     RERLTRAGHAPVPAPGSAIVSVPGLGARAAALAEQGITVSDRAGMLRAAFHLYNTESDV
                     DRLVAALGG"
     aSDomain        1290..2153
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9W68_RS24855_Aminotran_5.1"
                     /evalue="6.30E-24"
                     /label="B9W68_RS24855_Aminotran_5.1"
                     /locus_tag="B9W68_RS24855"
                     /protein_end="343"
                     /protein_start="55"
                     /score="76.5"
                     /tool="antismash"
                     /translation="AARASFARLVGVPATRVATGSSVATFTGLIAAALPDGAEVLTADG
                     DFSSLVNPFHVRPGLTVRTVPLERIAEHVTEATALVAVSAVQSADGRRADLTAVREAAR
                     AHGARTLVDASQAAGWDTFEAGAYDYTVAVGFKWLLAPKGTAFLTVPEEPGALSPLLAG
                     WVAGDSPFDSCYGPVTDLAPDARRYDESPAPLVWAGAQHALALLERIGTEAIGAHDVAL
                     ADRFRERLTRAGHAPVPAPGSAIVSVPGLGARAAALAEQGITVSDRAGMLRAAFHLYNT
                     ESDVDRL"
     gene            complement(2183..2695)
                     /locus_tag="B9W68_RS24860"
                     /old_locus_tag="B9W68_24910"
     CDS             complement(2183..2695)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018470511.1"
                     /locus_tag="B9W68_RS24860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_24910"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_030695054.1"
                     /transl_table=11
                     /translation="MNEATGPEQAPCTWPAERGAALPGGPVGHLVTRVARLHRTAAGRA
                     LRESGLYPGQEIVLGHLWEAGGPVRQSDLIKAVELDPSTVTKTLQRLEQSGHVTRAPDP
                     ADRRAVLVEATELSCALSRQVESAWAGLEEHALAGLTPADRAELARLLAAVEANLCDRA
                     GDCPEAG"
     misc_feature    2803..2813
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     gene            2867..3928
                     /locus_tag="B9W68_RS24865"
                     /old_locus_tag="B9W68_24915"
     CDS             2867..3928
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947424.1"
                     /locus_tag="B9W68_RS24865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_24915"
                     /product="alkene reductase"
                     /protein_id="WP_087776595.1"
                     /transl_table=11
                     /translation="MSTAFEPLDLSGTKLPHRIAMAPMTRSRSGAAGVPTALMAEYYAQ
                     RASAALIVTEGIQPSAVGQGYPDTPGLHSAGQVTGWRAVTSAVHAAGGRIFAQLMHAGR
                     IGHPSLLPEGEVNVAPSAVAATGQVYTADGPKDFVTPRELTDDEVRATLADYVTAARNA
                     VDAGFDGVELHGANGYLIHQFLAPGSNRRTDRWGGSVEARIRFAAEVTEAVAAEIGAAR
                     TALRLSPGNAFNSIEEPEPEPEYTALAARIAPLGLAYLHVAEDVPARRALTLRLRELFG
                     GPLMLNPADSPTGPEALALVEEGTADLVSYGQLFLANPDLPARLRAGGPFNEARRATMY
                     GGGAEGYTDYPAL"
     gene            complement(4101..4997)
                     /gene="thpD"
                     /locus_tag="B9W68_RS24870"
                     /old_locus_tag="B9W68_24920"
     CDS             complement(4101..4997)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010637026.1"
                     /locus_tag="B9W68_RS24870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_24920"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_023420017.1"
                     /sec_met_domain="PhyH (E-value: 5.5e-38, bitscore: 129.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVASERTDLYPSRTATEVTIPRKDPVVWSPQDAPGPITRSDLQS
                     YDENGFLAVEELITPEEVGVYRAELDRLVADPLIRADERAIVEPKSEKVRSVFEIHKIS
                     EVFAGLVRDERLLGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPR
                     MRAVSVSIALTENYDTNGGLMIMPGSHRTYVGCNGVTPKDNYKRSLQMQDAGIPSDEVL
                     TEFADRHGIKLFTGKAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENAAEEPFAA
                     PVRRPEFIGARDFTPVK"
     gene            complement(5001..5399)
                     /locus_tag="B9W68_RS24875"
                     /old_locus_tag="B9W68_24925"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947422.1"
                     /locus_tag="B9W68_RS24875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_24925"
                     /product="ectoine synthase"
                     /protein_id="WP_003947422.1"
                     /sec_met_domain="ectoine_synt (E-value: 9.6e-55, bitscore:
                     182.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIENTDRHVKSASGTWESKRIVLAKEKVGFSLHETILY
                     AGTETDMWYANHIEAVLCVEGEAELTNRETGETHWIEPGTMYLLNGHEKHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEED"
     gene            complement(5434..6705)
                     /gene="ectB"
                     /locus_tag="B9W68_RS24880"
                     /old_locus_tag="B9W68_24930"
     CDS             complement(5434..6705)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 2.5e-75.
                     Score: 245.5. Matches aSDomain:
                     nrpspksdomains_B9W68_RS24880_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="B9W68_RS24880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_24930"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_087776597.1"
                     /sec_met_domain="Aminotran_3 (E-value: 9.4e-80, bitscore:
                     266.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRSWPAVFDRAQGSYLYDEDGHTY
                     LDFFAGAGALNYGHNNPVLKRALIDYIERDGITHGLDMGTTAKRAFLETFQNVLLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVQDFLWFERLLEDQGSGLNTPAAVIVETIQGEGGINVARP
                     EWLRALADLCKRRDMLLIVDDIQMGCGRTGPFFSFEEAGITPDIVTLSKSIGGYGMPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWSDGQMEKQTLARGEQVEQALT
                     AILDEHGGESIVSIRGRGLVWGMEFADKSRAGAVSKRAFELGLLVETSGPEGEVVKLLP
                     PLTVSPDELDEGLRILARAVRETA"
     aSDomain        complement(5608..6621)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9W68_RS24880_Aminotran_3.1"
                     /evalue="2.50E-75"
                     /label="B9W68_RS24880_Aminotran_3.1"
                     /locus_tag="B9W68_RS24880"
                     /protein_end="366"
                     /protein_start="28"
                     /score="245.5"
                     /tool="antismash"
                     /translation="FDRAQGSYLYDEDGHTYLDFFAGAGALNYGHNNPVLKRALIDYIE
                     RDGITHGLDMGTTAKRAFLETFQNVLLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVQDFLWFER
                     LLEDQGSGLNTPAAVIVETIQGEGGINVARPEWLRALADLCKRRDMLLIVDDIQMGCGR
                     TGPFFSFEEAGITPDIVTLSKSIGGYGMPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDAYWSDGQMEKQTLARGEQVEQALTAILDEHGGESIVSIRGRGLVWGMEFA"
     gene            complement(6826..7332)
                     /gene="ectA"
                     /locus_tag="B9W68_RS24885"
                     /old_locus_tag="B9W68_24935"
     CDS             complement(6826..7332)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412174.1"
                     /locus_tag="B9W68_RS24885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_24935"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_087776599.1"
                     /transl_table=11
                     /translation="MKEMPEDLRLDHPGVEDGAAIWRIARDSRTLDLNSSYSYLLWCRD
                     FAGTSVVARDAAGEPVGFVTGYFRPERPHTLVVWQVAVDASQRGRGLAAALLDGLTDRL
                     REKGRLDGIETTITPGNTPSERLFTSYAKRHGAELEREVLFGAGLFPDGGHDPEVLYRI
                     GPFTD"
     misc_feature    complement(7373..7385)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(7399..7411)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     misc_feature    complement(7430..7441)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.51"
                     /tool="antismash"
     misc_feature    complement(7488..7506)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 18.88"
                     /tool="antismash"
     misc_feature    complement(7530..7545)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7540..7555
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7551..7566)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 18.42"
                     /tool="antismash"
     misc_feature    complement(7673..7686)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.78"
                     /tool="antismash"
     misc_feature    7673..7689
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            complement(7828..8943)
                     /locus_tag="B9W68_RS24890"
                     /old_locus_tag="B9W68_24940"
     CDS             complement(7828..8943)
                     /NRPS_PKS="Domain: Aminotran_5 (73-284). E-value: 5.1e-31.
                     Score: 99.8. Matches aSDomain:
                     nrpspksdomains_B9W68_RS24890_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947419.1"
                     /locus_tag="B9W68_RS24890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_24940"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_087776601.1"
                     /sec_met_domain="Aminotran_5 (E-value: 6.9e-24, bitscore:
                     82.9, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPLLDLTPLTAAHFANIERRVAALLSTAQDVVITQGEALLPLE
                     GCIRGVAGPDTTALNVITGPYGQTFGDWLRDCGARVVDLAVPYDSAVSAAQVAEALAEH
                     PETSFVSLVHAEAATGNTNPVAEIGRVVREHGALFMVDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWQRMAANPRAPRRSYLSLLDWKERWIDAGRTALLHAP
                     AQLEMVALQACVERIEDEGLETVIGRHTRAAAATRAGVKALGGGFAPYVREARHAAPVA
                     TTLRAPEGVDASELVARALAADPVLPLVAGGGALAKEMIRVNHYGTAAAGSAVHSCLSA
                     LGAALTDLTGRSVDPDAARRAATAAWERPAG"
     aSDomain        complement(8092..8724)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9W68_RS24890_Aminotran_5.1"
                     /evalue="5.10E-31"
                     /label="B9W68_RS24890_Aminotran_5.1"
                     /locus_tag="B9W68_RS24890"
                     /protein_end="284"
                     /protein_start="73"
                     /score="99.8"
                     /tool="antismash"
                     /translation="LRDCGARVVDLAVPYDSAVSAAQVAEALAEHPETSFVSLVHAEAA
                     TGNTNPVAEIGRVVREHGALFMVDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVS
                     AVSVSERAWQRMAANPRAPRRSYLSLLDWKERWIDAGRTALLHAPAQLEMVALQACVER
                     IEDEGLETVIGRHTRAAAATRAGVKALGGGFAPYVREARHAAPVATTL"
     gene            9018..10109
                     /locus_tag="B9W68_RS24895"
                     /old_locus_tag="B9W68_24945"
     CDS             9018..10109
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019982886.1"
                     /locus_tag="B9W68_RS24895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_24945"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_087776603.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 6.9e-16, bitscore:
                     56.8, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEDMVLHVKGRILVGPEEARDELWVVGGRITFTRPPGAREVRTV
                     EGWALPGLVDAHCHVGLDRHGPVAAEVAEKQALTDRDAGALLLRDAGSPSDTRWMDDRE
                     DLPRVIRAGRHIARTRRYIRNYAHEIEPDRLVAYVGQEARRGDGWVKLVGDWIDRGAGD
                     LTPCWPREAVEAAIAEAHRFGARVTAHCFAEDSLRDLVEAGIDCVEHATGLTEDTIPLF
                     AERGVAIVPTLVNIATFPDLAAGGEAKFPDWSAHMRRLYERRYDTVRSAWDAGIPVFVG
                     TDAGGSLPHGLVAAEVAELVTAGIPPLDALSATAWGARDWLGRPGLTEGSPGDLVVYAS
                     DPRADVRVLAAPRRVVVGGKVVA"
     misc_feature    10271..10286
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.95"
                     /tool="antismash"
ORIGIN
        1 cgcgtaccag accacgcccg aggcactcct cccgccggag ctgcgggacg gtaacccgct
       61 ggggctgccc cggcacgtgg acgggcgggc cgagcagggg gcctgagggc ggcaggggct
      121 cgtcagggcc ggtccggaat gcaccggtcc ggtcctgcgt tgcagcagag cggaggcacc
      181 ctccgtaccc tggtgtccgc acgcccccgg gagaccgctc ccgccacccg acctgcccgg
      241 tccccgggcc gaggagttga accgcagcca tgcgcgtcga gatctggtcc gacatcgcct
      301 gcccctggtg ctatgtagga aaggcgcggt tcgacaaggc gctcgccgcc ttcccgcacc
      361 gcgaccggat cgaggtcgtg caccgctcct tcgagctgga cccggcccga cccaagggcg
      421 accccgagct ggtcctgccg atgctcgcca agaagtacgg catgagcgag gcgcaggccg
      481 ccgaggccga gcggaacctc gggcagaacg ccgccgccga gggcctgccc tacctcaccg
      541 agggccgcga ccacggcagc accttcgaca tgcaccggct gctccacctc gccaaggcgc
      601 gcggccgcca ggaggaactg ctcgccgtgc tctaccgcgc caacttcgcc gagaccgaga
      661 ccgtcttcgg tgtcgacgag cggctcgtcg tcctcgccgt cgaggccggg ctggaggagg
      721 ccgaggcccg cgaggtcctc gccgacgagc agcggtacgc cgccgaggtc cgcgccgacg
      781 agcgcgccgc caccgaactc ggtgccaacg gcgtcccgtt cttcgtcctc gaccggcggt
      841 acggcgtctc cggcgcccag cccgccgacg tcttcacgca ggcgctcacc caggcgtggg
      901 agagccacgt ccccgcctcc gggctggcca ccccggccgg cgccgagggc ggcgaggtct
      961 gcggccccga cggctgcgag gtcccgaagg gctgaccgcg ccccggcacc ggcccggccc
     1021 gccacccgct ccggcgcggt ggcggacggg acccgtacca ccagggacgg ttcggcggtc
     1081 gccgcaccat ggacggctgc cgcgcgaggg ggcacagtgg ccgcatggat ctcgcccgca
     1141 gcctggccga agacgagttc aagccctccc tgacgcacct ggcgaccgcc agcaccggcc
     1201 tcctgccgct gagcgcggcc cgggccgcgc acgaggcggt cgacgccgcc gcggccggcg
     1261 ccccgcccga catgttcggc gcccgggacg ccgcccgggc ctccttcgcc cggctggtgg
     1321 gcgtccccgc cacccgcgtc gccaccggat cgtcggtggc gaccttcacc gggctgatcg
     1381 ccgccgcgct gcccgacggc gccgaagtgc tcaccgccga cggcgacttc agctcgctgg
     1441 tcaacccctt ccacgtgcgg ccgggactga ccgtgcgcac cgtgccgctg gagcggatcg
     1501 ccgagcacgt caccgaggcg accgccctgg tcgcggtcag cgccgtgcag tccgccgacg
     1561 gccgccgcgc cgacctcacc gccgtccgcg aggcggcccg cgcccacggc gcccgcaccc
     1621 tggtcgacgc ctcccaggcc gcagggtggg acaccttcga ggccggcgcg tacgactaca
     1681 cggtcgccgt cggcttcaag tggctgctcg ccccgaaggg caccgccttc ctcaccgtcc
     1741 cggaggagcc cggcgccctg tccccgctcc tcgcgggctg ggtcgcgggc gactccccgt
     1801 tcgacagctg ctacggaccc gtcaccgacc tggcccccga cgcgcgccgc tacgacgaga
     1861 gtcccgcccc gctcgtctgg gccggcgcgc agcacgccct cgccctgctg gagcggatcg
     1921 ggaccgaggc gatcggcgcc cacgacgtgg cgctcgccga ccgcttccgg gagcggctga
     1981 cccgcgccgg acacgccccc gtcccggccc ccggctcggc catcgtctcc gtgccggggc
     2041 tgggggcgcg ggcggcggcc ctcgcggagc aggggatcac cgtctccgac cgggccggga
     2101 tgctccgggc cgccttccac ctgtacaaca ccgagagcga cgtcgaccgg ctggtggcgg
     2161 cgctcggcgg ctgagcggtg cgctatccgg cctcggggca gtcgcccgcc cggtcgcaga
     2221 ggttggcctc caccgccgcc agcaggcgcg ccagttcggc gcggtcggcg ggggtgaggc
     2281 cggccagggc atgctcctca aggccggccc aggcggactc gacctgacgg ctcagggcgc
     2341 agctcagctc ggtcgcctcg accaggacgg cccgccggtc ggcggggtcg ggggcgcggg
     2401 tgacgtgccc ggactgctcc agccgctgga gggtcttggt caccgtggag gggtcgagtt
     2461 cgaccgcctt gatcaggtcg gactggcgta ccgggccgcc cgcctcccag agatggccga
     2521 ggacgatctc ctggcccggg tagagacccg actcgcgcag cgcccggccg gcggcggtcc
     2581 ggtgcagccg tgccacccgg gtgacgagat ggccgacggg cccgccgggc agggccgcgc
     2641 cccgctcggc gggccaggtg cagggggcct gctcggggcc ggtcgcctcg ttcatccacg
     2701 tctcccgctg ggtcgtcctc gtcccggtct ccgcccgact atacctttgg tcggccaagc
     2761 aatgggttac agtgactccc ggcccaattg cttggccgac catgcaatgt gtcggccggg
     2821 gccccgctct gcccggaacc acccgttccc ccactggaga ccgcccgtga gcactgcctt
     2881 cgagcccctc gacctgtccg gcaccaagct cccgcaccgc atcgccatgg cccccatgac
     2941 ccgcagccgc tccggcgcgg cgggtgtgcc gacggcgctg atggccgagt actacgcgca
     3001 gcgcgcctcg gccgccctga tcgtcaccga gggcattcag ccctccgccg tcggccaggg
     3061 gtacccggac acccccggcc tccattcggc cggacaggtg acggggtggc gcgcggtcac
     3121 ctccgcggtg cacgccgccg gcggccggat cttcgcccag ctcatgcacg ccggtcggat
     3181 cggccacccc tcgctgctgc cggagggcga ggtcaacgtc gccccgtccg cggtcgccgc
     3241 gaccggccag gtctacaccg ccgacggccc caaggacttc gtcacgccgc gtgagctgac
     3301 cgacgacgag gtccgcgcca ccctcgccga ctacgtcacg gccgcccgca acgcggtcga
     3361 cgccggtttc gacggggtcg agctgcacgg cgccaacggg tacttgattc accagttcct
     3421 cgcccctggc tccaaccgcc gcaccgaccg ctggggcggc tcggtcgagg cccgcatccg
     3481 gttcgcggcc gaggtcaccg aggcggtcgc cgccgagatc ggcgccgcca ggaccgccct
     3541 gcgcctgtcg cccggcaacg ccttcaacag catcgaggag cccgagccgg agccggagta
     3601 caccgcgctg gccgcccgga tcgccccgct cggcctcgcc tacctgcacg tcgccgagga
     3661 cgtcccggcc cgccgggcgc tcaccctgcg cctgcgggag ctgttcggcg gcccgctgat
     3721 gctgaacccg gccgacagtc cgaccggccc cgaggcgctg gccctggtcg aggagggcac
     3781 cgccgacctg gtcagctacg gccagctctt cctggccaac cccgacctgc ccgcgcggct
     3841 gcgcgccggg gggccgttca acgaggcccg gcgcgccacg atgtacggcg gcggggccga
     3901 gggatacacc gactacccgg cgctctgacc tgcgcacggt gcggaaaggc ccgtaaacgc
     3961 aaccggaccg ggagcggccc gcctgtggcc gcccccggtc ccgtggcccg ggcgccgtgt
     4021 gcgccggtgc gtgaggagtc cgctgtcagc ggctcgaccg gtgtgcctgc tccacctggg
     4081 tgaagcggga cgtcaccggc tcacttcacc ggggtgaagt cacgcgcccc gatgaactcc
     4141 ggacgccgca ccggcgccgc gaagggctcc tcagcagcgt tctcgacgct gttgaagacg
     4201 atgaacacgt tgctgcgcgg gtacggggtg atgttgtccc cggagccgtg catggcgttg
     4261 cagtcgaacc aggtggccga cccggccttg cccgtgaaga gcttgatgcc gtgccggtcg
     4321 gcgaactcgg tcagcacctc gtcggagggg atgcccgcgt cctgcatctg gagggaccgc
     4381 ttgtagttgt ccttcggcgt cacgccgttg caccccacgt acgtgcggtg cgagcccggc
     4441 atgatcatga ggccgccgtt ggtgtcgtag ttctcggtca gcgcgatcga gacggacacc
     4501 gcgcgcatcc tcggcagccc gtcctcggcg tgccaggtct cgaagtccga gtgccagtag
     4561 aagccggagg cgccgaagcc cggcttcacg ttgatccggg actggtggac gtagacgtcc
     4621 gagccgagga tctgccgggc gcggcccagc agccgctcgt cacggaccag gccggcgaag
     4681 acctcgctga tcttgtggat ctcgaagacc gagcggacct tctccgactt gggctccacg
     4741 atggcccgct cgtcggcccg gatgagcggg tcggcgacca gccggtccag ttcggcccgg
     4801 tagacgccga cctcctcggg ggtgatcagc tcctcgacgg cgaggaagcc gttctcgtcg
     4861 tacgactgca ggtcggaccg cgtaatgggt ccaggggcgt cctgcgggga ccacaccacg
     4921 gggtccttgc gcgggatcgt cacctcggtt gcagtccggc tcgggtagag atcggtgcgt
     4981 tccgacgcga cagtcatgac tcagtcctcc tcggtcagca gcgggtagac gccgttctcg
     5041 tcgtggtcct cccgtccggt cacgggaggg ttgaagacgc agacacagcg aaaatctgtc
     5101 ttcggcctca gggtgtgctt ttcgtggccg ttcagcaagt acatcgtgcc gggctcgatc
     5161 cagtgggtct cgcccgtctc gcggttggtc agctcggcct caccctcgac gcagagcacg
     5221 gcctcgatgt ggttggcgta ccacatgtcg gtctcggtgc ccgcgtagag gatggtctcg
     5281 tgcagcgaga agccgacctt ctccttggcg agcacgatgc gcttgctctc ccaggtgccg
     5341 gacgccgact tgacgtgccg gtcggtgttc tcgatgtcct tgaacgatcg gacgatcaca
     5401 gtgtggtcgt gccctttctt ccgtcgttgt gtgtcaggcc gtctctcgga cggcgcgggc
     5461 gaggatgcgc agaccctcgt ccagctcgtc cgggctcacg gtgagcggcg gcagcagctt
     5521 gaccacctcg ccctccggcc cggaagtctc gaccagcaga cccagctcga aggcgcgctt
     5581 ggagacggcg cccgcgcggg acttgtcggc gaactccatg ccccagacca gaccccgccc
     5641 gcggatggag acgatggact cgccgccgtg ctcgtcgagg atggcggtga gcgcctgctc
     5701 gacctgctca ccccgggcga gggtctgctt ctccatctgg ccgtcgctcc agtacgcgtc
     5761 cagcgcggcg gcggcggtga cgaaggccgg gttgttgccc cggaaggtgc cgttgtgctc
     5821 gcccggctcc cagatgtcca gctccggctt gaacaggcag agcgacatgg gcatgccgta
     5881 gccgccgatg gacttcgaca gggtgacgat gtccggggtg atgcccgcct cctcgaagga
     5941 gaagaacggg ccggtgcggc cgcagcccat ctggatgtcg tcgacgatca gcagcatgtc
     6001 gcggcgcttg cacaggtcgg ccagggcgcg cagccactcg gggcgggcca cgttgatgcc
     6061 gccctcgccc tggatggtct cgacgatgac cgcggccggg gtgttgaggc ccgagccctg
     6121 gtcctccagc agccgctcga accagaggaa gtcctggacg gtgccgtcga agtagttgtc
     6181 gaacggcatc ggcgtgccgt gcaccagcgg gatgccggcc ccggcgcgct tgaaggcgtt
     6241 gccggtcacg gcgagcgagc ccagcgacat gccgtggaag gcgttggtga aggagacgat
     6301 ggactcgcgg cccttgacct tgcgggccag cttcagcgcc gactcgacgg cgttggtgcc
     6361 cgtcgggccg gggaacatga ccttgtacgg caggtcacgc gggcgcagca gcacgttctg
     6421 gaaggtctcc aggaacgcgc gcttggcggt ggtgcccatg tccaggccgt gggtgatgcc
     6481 gtcgcgctcg atgtagtcga tcagcgcgcg tttcagcacc gggttgttgt ggccgtagtt
     6541 gagggcgccg gcgccggcga agaagtcgag gtaggtgtgg ccgtcctcgt cgtacaggta
     6601 gctgccctgg gcccggtcga agacggcggg ccagctgcgg cagtagctcc gtacctccga
     6661 ctcaagggtc tcgaagacgc tcagggcggg cggggtgatg gtcacgactg ctctcctggg
     6721 agatgggggg tgaagcgggg cggaagtcag ggatacgtgc ggaagggcgg ccgggggctt
     6781 accttccggc cggacctgga gttgctgtgc gggcgttcgc gctggtcagt cggtgaacgg
     6841 tccgatgcgg tacagcacct cggggtcgtg ccccccgtcg gggaagagtc cggcaccgaa
     6901 gaggacctcg cgctccagct cggcgccgtg gcgcttggcg taggaggtga agaggcgctc
     6961 ggagggcgtg ttgcccgggg tgatggtggt ctcgattccg tccagccgcc ccttctcgcg
     7021 gagccggtcg gtgagcccgt ccagcagggc ggcggcgagg ccgcgtccgc gctgcgaggc
     7081 gtccacggcg acctgccaga cgacgagggt gtgcgggcgc tcgggccgga agtacccggt
     7141 cacgaacccg accggctcgc cggcggcgtc gcgtgccacc acggaggtgc cggcgaagtc
     7201 gcgacaccac aggaggtagc tgtaggagga gttgaggtcg agggtgcgcg agtcgcgggc
     7261 gatgcgccag atggctgccc cgtcctcgac gcccgggtgg tccaggcgca aatcctctgg
     7321 catttccttc atggtggatc ggccacgttc aaggtcggct tgtgcggcgg tcatgcaaat
     7381 tgaatttacc gatcggctct gaaaaatgca tcgcgggtca gggttacatg acagcgcttt
     7441 ctgtgttatc aggcgcgggg gtgcgcctgc gcgaacggcc gtccgaagat ggtgatttgt
     7501 cctgcatata aggtgcgaaa cgggcaccgt gtggagtcag tcacaagtgc gtaacgcacg
     7561 cgtgacgcgt ccgaattacg cggtttcggt gtccggaaaa tcgatgcgtt tagcttcggg
     7621 gagagcgggc agacgaatag gggaaggtga gagcgaaaga attcggaatt ccctgtaata
     7681 ggtctaccga taagaatatg gttcgctgtc cgcgccgaag tcggtgcgga cttcggtgtg
     7741 cccggggacg gaaaagcgcg acgggccgcc ccgcgcacgg cgtgcgccgc gcaccggacg
     7801 gcccgccggg gccggtgggg gcggggatca gcccgcgggg cgctcccagg ccgcggtcgc
     7861 cgcgcgccgc gccgcgtccg ggtcgaccga ccggcccgtg aggtcggtca gcgccgcccc
     7921 cagcgcggag aggcaggagt ggaccgcgga gccggccgcg gccgtgccgt agtggttgac
     7981 ccggatcatc tccttggcca gcgctccgcc gcccgccacc agcggcagca ccgggtccgc
     8041 cgccagcgcc cgcgccacca gttccgaggc gtccacgccc tccggcgccc gcagcgtggt
     8101 cgccaccggc gccgcgtggc gcgcctcgcg aacgtacggc gcgaaaccgc cgccgagcgc
     8161 cttcacgccg gcccgggtgg ccgccgccgc ccgggtgtgc cgcccgatca ccgtctccag
     8221 gccctcgtcc tcgatccgct ccacgcacgc ctggagcgcc accatctcca gctgggcggg
     8281 ggcgtgcagc agggcggtgc ggcccgcgtc gatccaccgc tccttccagt ccagcagcga
     8341 gaggtacgag cggcgcgggg cccgcgggtt ggccgccatc cgctgccagg cccgctcgct
     8401 caccgagacg gccgagaccc cggccgggcc gcccatcgcc ttctgggcgc cgatcacgca
     8461 gaggtccacg ccccaggcgt ccgggagcag cggctcggcg ccgaccgagg ccaccgcgtc
     8521 caccatgaag agcgcgccgt gctcgcgcac cacccggccg atctcggcca ccgggttggt
     8581 gttgccggtc gcggcctcgg cgtggaccag cgagacgaag ctggtctccg ggtgctccgc
     8641 cagcgcctcg gcgacctggg cggcgctcac cgcggagtcg tagggcaccg ccaggtcgac
     8701 cacgcgcgcc ccgcagtcgc gcagccagtc gccgaaggtc tgcccgtagg ggccggtgat
     8761 cacgttgagg gcggtggtgt ccgggcccgc cacgccgcgg atgcagccct ccagcggcag
     8821 cagcgcctca ccctgggtga tgacgacgtc ctgcgcggtg gagagcagcg ccgcgacgcg
     8881 gcgctcgatg ttcgcgaaat gcgcggcggt cagcggggtc aggtccagga gggggtgggt
     8941 cacgggggtg ctctcttcgc cggtcgatcg tcgggctgcc ggtccgagcg tacccaccgg
     9001 gtcctacgct gccgtgcatg agcgaagaca tggtgctgca cgtgaagggc cggatactgg
     9061 tcggtcccga ggaggccagg gacgaactct gggtggtggg cgggcggatc accttcaccc
     9121 ggccgccggg cgcccgcgag gtgcggaccg tcgaggggtg ggcgctgccg ggcctggtcg
     9181 acgcccactg ccacgtcggc ctggaccggc acggcccggt cgccgccgag gtcgccgaga
     9241 agcaggcgct caccgaccgc gacgccgggg cgctgctgct gcgcgacgcc gggtcgccca
     9301 gcgacacccg ctggatggac gaccgcgagg acctgccccg cgtcatccgg gccggccgcc
     9361 acatcgcgcg cacccgtcgc tacatccgca actacgccca cgagatcgag ccggaccgac
     9421 tcgtcgccta cgtcggtcag gaggcgcggc gcggcgacgg ctgggtcaag ctggtcggcg
     9481 actggatcga ccggggcgcg ggcgacctga cgccgtgctg gccccgcgag gcggtcgagg
     9541 ccgccatcgc cgaggcgcac cggttcggcg cccgcgtcac cgcccactgc ttcgccgagg
     9601 actcgctgcg cgacctggtc gaggcgggca tcgactgcgt cgagcacgcc accgggctca
     9661 ccgaggacac catcccgctc ttcgccgaac gcggcgtcgc catcgtcccc accctggtca
     9721 acatcgccac cttccccgac ctcgcggcgg gcggcgaggc gaagttcccc gactggtcgg
     9781 cccacatgcg gcggttgtac gagcgccggt acgacacggt ccgctccgcc tgggacgcgg
     9841 gcatcccggt cttcgtcggc accgacgcgg gcggcagcct cccgcacggc ctggtcgccg
     9901 ccgaggtcgc cgaactggtc accgccggca tcccgccgct cgacgccctc tccgccacgg
     9961 cgtggggcgc ccgcgactgg ctcggccgcc cgggactgac cgaggggtcc ccgggcgacc
    10021 tggtggtcta cgcgagcgac ccgcgcgccg acgtccgggt gctggcggcg ccccgccggg
    10081 tggtggtcgg cggcaaggtc gtcgcctgac cgcgagaagc gtgttgacgg gccgttacgg
    10141 gggccggagc agcgtcgttg tgctccggcc cccgccgcgt ctccgcgccc gcgtacgccg
    10201 tccgtacgcc ccttgggggg ccggatgcgg cggtcagggg tgacatgggg gaacgaccgt
    10261 gccgatcggt tcgaacgtct gcaccaaaac caccctttgg agtgaagtca ccccaggttg
    10321 tcgccccttc acccggagtg cgtaaagatt ccggcaacga cgtcgtcggc ggccgcctgt
    10381 ccccaggtgc ggccccggc
//
