LOCUS       NZ_KZ195583            10513 bp    DNA     linear   CON 07-SEP-2024
DEFINITION  Streptomyces sp. CS227 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ195583
VERSION     NZ_KZ195583.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS227
  ORGANISM  Streptomyces sp. CS227
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6794013)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794013)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6794013)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 370.61x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188355.1-RS_2024_09_07
            Annotation Date                   :: 09/07/2024 02:15:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,017
            CDSs (total)                      :: 5,954
            Genes (coding)                    :: 5,785
            CDSs (with protein)               :: 5,785
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 72 of 169
            Pseudo Genes (incomplete)         :: 127 of 169
            Pseudo Genes (internal stop)      :: 12 of 169
            Pseudo Genes (multiple problems)  :: 41 of 169
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:26:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2710668
            Orig. end                         :: 2721181
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195583.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
FEATURES             Location/Qualifiers
     region          1..10513
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="14"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10513
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10513
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5513](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5513
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    440..453
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.32"
                     /tool="antismash"
     misc_feature    complement(465..475)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    complement(570..583)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.04"
                     /tool="antismash"
     misc_feature    complement(577..594)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.53"
                     /tool="antismash"
     gene            complement(1316..1615)
                     /locus_tag="B9W68_RS11725"
                     /old_locus_tag="B9W68_11750"
     CDS             complement(1316..1615)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007388782.1"
                     /locus_tag="B9W68_RS11725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_11750"
                     /product="hypothetical protein"
                     /protein_id="WP_087773516.1"
                     /transl_table=11
                     /translation="MTLQNVLRLLPWSGPDGKPCYLSTDDSGGYMSRLADNIEAVQLGM
                     ATDLLERAAGVLGDQDAGRDDVRLMLTEVTRALRDVVRVATSRGHLLAVSELQS"
     gene            complement(1612..2343)
                     /locus_tag="B9W68_RS11730"
                     /old_locus_tag="B9W68_11755"
     CDS             complement(1612..2343)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950163.1"
                     /locus_tag="B9W68_RS11730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_11755"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_087777819.1"
                     /transl_table=11
                     /translation="MTSRRLVVADDLRRHITTGRIGPGERLPSESALADRYKVSTGTLR
                     SALAMLQGEGFVEKIHGKGNFVRRPLRKVTYVGGWGTLDPRAAADVDLRVTVRTTVLPA
                     RGHLTALLKVDDGSPLTEFFRLSYEGESPLSLARVYVPRGLTPATALSNRSSRAWAEAG
                     FSVPDPPPTEVHETVSARLPTPDEASSLRINTALAVLAITRVAADNTGRVIEAALLVFP
                     GNRVDAVFSTHHVIEERQAHR"
     gene            complement(2352..2576)
                     /locus_tag="B9W68_RS31705"
                     /old_locus_tag="B9W68_11760"
     CDS             complement(2352..2576)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950162.1"
                     /locus_tag="B9W68_RS31705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_11760"
                     /product="hypothetical protein"
                     /protein_id="WP_087773517.1"
                     /transl_table=11
                     /translation="MSNWALGGALVSNVEITAATVLRGDIIQVGGRACRVCDLFQLPRG
                     AKRLLFESGEPLTIDGRTRLVAVRTTRRR"
     gene            complement(3100..3378)
                     /locus_tag="B9W68_RS31710"
                     /old_locus_tag="B9W68_11775"
     CDS             complement(3100..3378)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950159.1"
                     /locus_tag="B9W68_RS31710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_11775"
                     /product="hypothetical protein"
                     /protein_id="WP_003950159.1"
                     /transl_table=11
                     /translation="MPASRVSAEAIAARLSVVGLTTRVEERDRCMSVEAEVPESLSAES
                     WREVLEVVAEADRFGLHATSLNGRTLWAVVRKAVPPKGDVGGPSYQR"
     misc_feature    complement(3334..3336)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    3442..3456
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.6"
                     /tool="antismash"
     gene            3458..4213
                     /locus_tag="B9W68_RS11755"
                     /old_locus_tag="B9W68_11780"
     CDS             3458..4213
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987974.1"
                     /locus_tag="B9W68_RS11755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_11780"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_087773520.1"
                     /transl_table=11
                     /translation="MSEQPPYLRIADELRRRIAEHVWEPGDRLPSRAQIGQEFGVGENV
                     VRRAQELLISQGVLEGRAGSGTYVAEPRQRVRIVRSSAREQPEGSPFRADMKAVGRQGD
                     WESRTDAKVPAPAAIAARLGIAEGELCVRTAYEFLADGRPVQLSTSWEPYDLTAGTLVV
                     LPEGGPRAGAGVVNRMAAIGVTVSHAVEQPEPRQATAEEASLLGVQKAGLVTHIRRTYY
                     SDDGRPVETADIVIPAAHCEIVYEIPISR"
     assembly_gap    4627..4651
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     misc_feature    4741..4756
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.73"
                     /tool="antismash"
     misc_feature    complement(4753..4762)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 17.48"
                     /tool="antismash"
     misc_feature    complement(4859..4873)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.2"
                     /tool="antismash"
     misc_feature    complement(4872..4889)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: medium, score: 26.98"
                     /tool="antismash"
     gene            5001..5513
                     /locus_tag="B9W68_RS11765"
                     /old_locus_tag="B9W68_11790"
     CDS             5001..5513
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950154.1"
                     /locus_tag="B9W68_RS11765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_11790"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_003950154.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2e-06, bitscore:
                     26.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKFDRSIALGLASAALAVSASTASAAAGTPQTAAGTTTEVITLA
                     DGTEVGQVTHHKRGDGTQPPAELGDPEEWGVATIEMDDSTIKPLVEACTNPPSGGKWCY
                     GWYLTGSTATQKYCYSNYYHGTKTHKSSVTIANGSVSSGWVAKGDYSNANRTAGAAYMC
                     NTYYATK"
     gene            5604..6275
                     /locus_tag="B9W68_RS11770"
                     /old_locus_tag="B9W68_11795"
     CDS             5604..6275
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950153.1"
                     /locus_tag="B9W68_RS11770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_11795"
                     /product="hypothetical protein"
                     /protein_id="WP_003950153.1"
                     /transl_table=11
                     /translation="MVHKRTGKTITHLLRNYLAPTLAALATGALLGLIGPGAAKWDNSF
                     GAALSLIFSSGWPWACYAFLVGFLCRSKTMAGILSFTGLTTGVTVYYLFKYMNPYVPPG
                     ATNTTGEGFSSGVVLWGIYALVLGLPMGLVGYLARRQGLVALPFRLVVPAIAFFETTLR
                     LDVESDGRDYVTLLTWNVTRYAAAATALVISGHAAWSFWCRHRSRSTENGAHRTHRLPG
                     G"
     assembly_gap    6333..6357
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7388..7762)
                     /locus_tag="B9W68_RS11780"
                     /old_locus_tag="B9W68_11805"
     CDS             complement(7388..7762)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018471723.1"
                     /locus_tag="B9W68_RS11780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_11805"
                     /product="VOC family protein"
                     /protein_id="WP_087773523.1"
                     /transl_table=11
                     /translation="MNTPTAGAPLPVTTLLAVAPVTDLARATTWYTHLLGRPADTHPMP
                     SLTEWQPTPDTWLQLFHSPEHAGSTLINIGVPDLDRALGELAERGLEADAPQEGADGTV
                     RFAALCDPEGNRVTLIEGRG"
     gene            complement(7879..8358)
                     /locus_tag="B9W68_RS11785"
                     /old_locus_tag="B9W68_11810"
     CDS             complement(7879..8358)
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404291.1"
                     /locus_tag="B9W68_RS11785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_11810"
                     /product="cold-shock protein"
                     /protein_id="WP_030697846.1"
                     /transl_table=11
                     /translation="MLKTGKILRFDEVRGYGFIVPNEGGEDVFMHANDLVDEKYLYQSG
                     REVEFFEEMGEKGPKASQIRLVYRSSPRRSTPAVGFSSGGPDAPRITNPGVDEPDTELD
                     EYDDQDEADTLTVAELRTELTDLLLDTDDTLTADQIKRIRTRVTELARVHGWVAS"
     gene            8625..9494
                     /locus_tag="B9W68_RS11790"
                     /old_locus_tag="B9W68_11815"
     CDS             8625..9494
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018471724.1"
                     /locus_tag="B9W68_RS11790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_11815"
                     /product="hypothetical protein"
                     /protein_id="WP_179230655.1"
                     /transl_table=11
                     /translation="MWVSKKQVREMFELMGHGQPVVLTSPMASVKKLAELACTAQLFGY
                     EYIDVRQGGGRNTALHLYLAPDPSPAARQRAAANWQQYPQAADDGPLPPYPSDMFELLK
                     ARINFDLTGRLTETNRLVMAGLALTVAAGLMPLRDVPTAFAVGFWVVTMGALGVAVLVQ
                     RQRNAKFAAYLEGYGFQRVTDQEGRARYLPPGAHTGWGAPVQQPGPYAQPQQPQPAPYG
                     YPQPGRPQQPAPYGYPQQPQQPQPGPYGHPQQGQPQPPQPGQWQHQPGQWPQQAPQQPG
                     PHGQPPQK"
     misc_feature    9582..9595
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.22"
                     /tool="antismash"
     gene            9585..10286
                     /locus_tag="B9W68_RS11795"
                     /old_locus_tag="B9W68_11820"
     CDS             9585..10286
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404295.1"
                     /locus_tag="B9W68_RS11795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_11820"
                     /product="hypothetical protein"
                     /protein_id="WP_256968053.1"
                     /transl_table=11
                     /translation="MSKHLGPEAGPSHVDPSLIRPRRGVLAGVWVAAGLLLLGAVAGFV
                     LTLVSAVDAIDREQAFRSGGSARVEVTAGGEPAVYGQAPVPEGAECTLDGPGEAKFSPY
                     GARYTVKLNRTTWVRLLRIEADTPGTYTLRCTDPAGSATFAPGDGAGLGALANTLLLRS
                     ALPGLAGLALAGVAIALIVKRSRHRNRLAAEALGRSGPPPHGNGPSHGPGGPAGPQHDP
                     WQPPPGAPRKE"
ORIGIN
        1 cccaccggac ccacttcggc agaacgcgag ccagggaatc catagccatg acggaaaaca
       61 catcaggact ggccggcacg atgaagtaat cagagataga tacaagattc tgattgatcg
      121 aaccaaggcc cgggctcaag tcaagaatta catagtccgc cttgtatttc tctgcggtga
      181 tttggaacag gtgcgagaaa ctacccggga ggttcttcag ggcttggata gattcagaaa
      241 gctgctgcgc aacaccgagg gagacttcat cttccgccag gccgacatga ccaggaatca
      301 aaaacagccc ttcccgtcca gtaactggca cgcaatccac aggaacaaga gggcgagggc
      361 gtgactgaaa agccgggtcc agagcgtcgc gaagattctg gttcggatga tctgagtaga
      421 acttgtccag cgcatcgact tgagtcagat ccagaaccat gccggtgaga ttgcattggg
      481 ggtctgcatc gaccatgatg acggtcttac cccgctccgc aagcatccaa gaaagattaa
      541 agcaagtggt cgtcttactg acgccgcctt tattgttgaa catggcgatt acttctgcca
      601 caagtacccc cctgattgcg acccatacgc tgagtcttgc cgcggctcgt gtttctcatc
      661 gtagccatga tctggccgtg tgtgaccaca aagtcggata gccaggcaag ttgccggaat
      721 ccccctgtcc cccggcagct gctggccacc gtctgaagag tgcagcgccg ccaacgtgcg
      781 agcgccctcc cgggccgtcc tcgggccgtg gaagggcgct caatggtgac caacgtcgac
      841 agctgccgac agctcagcag caggtcaggg cgttgatcga taaggcggcc gcaggtcacg
      901 gccgccggtg atcagttgtg gctgggcagc agcccgacag gcgcatcatg gagcatgggg
      961 attgatgctc tgaagcaacg gggaggtgac gagcgtcagc tcgccgcgct ggacgacctc
     1021 gcaaggatgc tgagtgagtg cgcagccacc cccggcagtt atctttggtc cgggggcgat
     1081 tgaagcgcac gccacgactg ccgggtaagt atccgaccga gcggagggct gggccatctc
     1141 tgggccgtgc gaggtcaacc gaggccgacc accaacggca gaagcccacg gcatctgagc
     1201 tggtcagagc cgtgggcttc ttcagtcccg ctggtcagcg aaaccgatga tcagtagacg
     1261 ggcttctgag cactgacaag aggccccctc agggacgccg cctggaccgt gcccattagg
     1321 actggagttc gctcaccgcc aggagatgac cgcggctggt tgccacgcgg accacgtctc
     1381 gtagggctcg ggtcacttcg gtcagcatga gccgcacgtc atcgcggccg gcgtcctggt
     1441 cgccgaggac tcctgcggcc cgctccagaa gatcggttgc catcccgagc tgtaccgctt
     1501 cgatgttgtc cgccaggcgg gacatgtagc cgccgctgtc atcggtgctc aggtagcagg
     1561 gcttgccgtc cgggcctgac cacgggagga gtcgtagtac gttctgcagc gtcatctgtg
     1621 cgcctgcctt tcttcgatca cgtggtgggt gctgaagacg gcgtcgacac gattcccagg
     1681 gaacaccagt agcgccgcct caatgactcg cccggtgttg tctgccgcaa cgcgggtgat
     1741 cgcgaggacg gccagggctg tgttgatccg caggcttgac gcctcatccg gcgttgggag
     1801 acgggcggac accgtctcgt gcacttcggt cggcggcgga tccggcacag agaaaccggc
     1861 ttccgcccac gcccttgagg accgattgct gagcgccgtg gccggtgtga ggcctcgcgg
     1921 tacgtagacg cgggccagac tgagtgggga ctctccctcg tagctgagcc tgaaaaattc
     1981 ggtcagtggg cttccgtcgt ccaccttcag caacgctgtc aggtggcccc gcgcgggaag
     2041 caccgtagtt cggaccgtca cgcgcaggtc cacgtctgcg gcagcccgag gatccagcgt
     2101 gccccatcca ccgacgtacg tgaccttgcg cagcgggcgg cggacgaagt tgcccttgcc
     2161 gtggatcttc tcgacgaagc cttctccctg gagcatggcc agggcgcttc gcagtgtgcc
     2221 cgtgctgacc ttgtaccggt cggcgagggc ggactcagac gggagccgtt caccgggtcc
     2281 gattcgaccg gttgtgatgt ggcgcctcag gtcgtcagcg acgacgaggc gacgagacgt
     2341 catggactgt gtcaccgcct tctcgtcgtc cgcacggcga cgagccgggt gcgcccgtct
     2401 atggtcagcg gctctcccga ctcgaacagg agtcgcttcg cgccccgggg caactggaaa
     2461 aggtcgcaca cccggcaggc tcggccgccg acttgaatga tgtcgccccg cagaacagtg
     2521 gccgccgtga tctcgacgtt ggataccagc gcacccccga gggcccagtt actcaccgct
     2581 tcaccgccgg ccgcgtggag ttgtccggcg cagtgtggca ggggcaggcg cacgtctcat
     2641 agatcaccgg caggtcgacc ggcgccacgg atggcgacgc ttccgtacag gccgcgtgag
     2701 tgccaaccct gcatgcagtc gaccggtagg cagcagcccc ggattccgat acatggagct
     2761 ggtgacgagg aggcattagc gagcacctgc cagagccaag aatgcctccg ccgacagccg
     2821 cggcgccaca accaccaagc gctggcgtgc acgctgccgc tcccaggcca gcacgtacgg
     2881 ccggacgagc gcgttgtcct cgcccaggag caggtctagg cggtcagcgg tgtccggcat
     2941 ccactccggg actgtcggcg gttctacggt gtccgcggga ggcatcgcga cagccgtccg
     3001 ggacggcgtt aaggctcggc ggtgcctgcc cttggggaag taccgcgctc tggcgagcgt
     3061 gacggcacgg cggatacggt tgagcacgct gctcagctcc tatcgctgat agctcggtcc
     3121 cccgacatcg cccttcgggg ggaccgcttt gcgtacgacc gcccaaaggg tgcggccgtt
     3181 caagctggtg gcgtgaagcc cgaatcgatc ggcctccgcc acgacttcca gcacctcccg
     3241 ccatgactcg gcggagagcg attccggcac ctccgcctcg accgacatgc accggtcgcg
     3301 ctcctccaca cgcgtggtga gcccgaccac tgataaccgg gcggcgatgg cctccgcact
     3361 cactcgcgaa gcgggcacag ggaagcctcc gtcactcggc gaacactttg agtgaagctg
     3421 gttaactaga ttagagctag tggactagat tttccatatg tctgagcagc cgccctatct
     3481 ccgcatcgcc gacgagctac ggcggcggat cgcggagcat gtgtgggaac cgggggaccg
     3541 gcttccttcc cgcgcacaga tcggccagga gttcggcgta ggcgagaacg tcgtccgcag
     3601 agctcaggag ctactgatct cgcaaggcgt gttggagggc cgagccggtt cgggcaccta
     3661 cgtcgccgag ccccggcagc gcgtacgtat agtgcggtcg tcggcgcgcg agcagcccga
     3721 agggtccccg ttccgggcgg acatgaaggc cgtcggcagg cagggcgact gggagagcag
     3781 gaccgacgcc aaggtgccgg ctccggcggc gatcgccgcc cggctcggta tcgccgaggg
     3841 cgagctgtgc gtccggaccg cgtacgagtt cctggccgac ggcaggccgg tgcagctctc
     3901 gaccagttgg gagccgtacg acctcaccgc cgggactctc gtcgttctcc ccgagggagg
     3961 accgcgcgca ggcgcgggcg tcgtgaaccg catggccgcg atcggcgtca cggtcagcca
     4021 cgccgtggag cagccggagc ccaggcaggc gaccgccgag gaggcctcgc tactcggcgt
     4081 ccagaaggcc ggactcgtca cacacatccg gcggacgtac tacagcgacg acggtcggcc
     4141 cgtggagaca gcggacatcg tgatccccgc ggctcactgc gagatcgtct acgagatccc
     4201 gatcagccga taggggctct tggcataccc ctgctcgctg cgtctcattc acaccccact
     4261 gacagccgag catggttagg atgccaacgg agctgcgctc agctcggcac tgccgagtct
     4321 cgcgctgtcc ctctccgcag ccgtcatctg caaggtcctg gcatcgaagt tgaacgacga
     4381 cgaccaagac tcgaagacga acgacgagga gaaccgtatg gctgatgcat cgaagttgag
     4441 cagcatgaag cagtggtcca gcgccatctg tgacgtacta ggagaccttg caatcggctt
     4501 cgcgggtggg gcagtgtcga gctgagcgca gctcttcgcg tttaagaccg tgtcctatgt
     4561 ggtgagtctg ggtagggctt caacgtgggt caggggacgt ggagtgggat tgttccggac
     4621 gggttgnnnn nnnnnnnnnn nnnnnnnnnn ncgtacgact caccacatag gacacggtct
     4681 tagtagggcg cttaacccag ccgggccctt cccgaccttc gcacgactgg aaacgctacg
     4741 atcaccgcat ggtcaaaaat tagccacacc atcgcaacta attcttcaca cgcccgaagg
     4801 cgcaatgacg gaacaatgca ccagaagatc cgctttcgag ctggcgagcg ggaactcgaa
     4861 attacttaat atatcctcat aagggaataa tcaggcaagt tgacttctag ccactgaacg
     4921 caacgctctc gcgccgccag gcccgctttc atgcatgatc tacgcgagcc ccacccgggg
     4981 actcacagga gggggatccg atgagaaagt tcgacaggtc gatagctcta ggtcttgcga
     5041 gcgcggcgct ggccgtaagc gcttctacgg cgtcggcggc agcaggcacc ccgcagacgg
     5101 cggccggcac cacgaccgaa gtgatcacgc tggcggacgg caccgaggtc ggccaggtca
     5161 ctcaccacaa gcggggcgac ggcacgcagc cgccggcgga actgggcgac cccgaggagt
     5221 ggggcgtggc cacgatcgag atggacgatt cgaccatcaa gcccttggtg gaggcgtgca
     5281 ccaacccgcc cagcggcggc aaatggtgct acggctggta cctcaccggg tcaactgcca
     5341 cccagaagta ctgctattcg aactattacc acggcacaaa gactcataag tcgtcggtga
     5401 ccattgccaa cggatcggtc tccagcgggt gggtagccaa gggcgattac tccaacgcca
     5461 acaggacagc gggcgcggct tacatgtgca atacgtacta cgccacgaag tagaagctgc
     5521 attctcacac ccggcgggcc actcgtcact cgcggaaaac cgccgcgcgt ggcgagtggc
     5581 cccgctgatg ccatctgccg aaattggttc acaagcgaac aggaaagacg atcacccacc
     5641 ttctgcgaaa ttacctcgcg ccgaccctag ccgctctcgc aacaggcgcg cttctgggcc
     5701 tcatcggccc cggggccgcc aagtgggata attctttcgg agccgccctg agtcttattt
     5761 tctccagcgg ctggccgtgg gcctgttacg ccttcctggt aggattcctc tgtcgatcaa
     5821 agaccatggc gggaattctt tccttcactg gactgactac cggcgtcacc gtttactatc
     5881 ttttcaagta catgaacccc tacgtgccgc cgggggcgac aaacaccacc ggcgaagggt
     5941 tttcttctgg agttgttctt tggggaatct acgcgctcgt gctcgggctg cccatgggcc
     6001 tcgttgggta ccttgcccgc cgccaaggac tcgtcgcgct gcccttccga ctcgtcgtcc
     6061 cggcgatcgc gttcttcgag accactctac gacttgacgt agaatccgac ggacgggatt
     6121 acgtcactct tctcacctgg aatgtgacca ggtatgcggc agccgctaca gccctggtca
     6181 tctccgggca cgccgcctgg tccttttggt gccgccaccg tagtcgctcg acagagaatg
     6241 gcgcgcaccg cacgcaccgt cttcccggtg gatgagcttc ttcagcgttg ctgcggccgc
     6301 taagaccgtg tcctatgtgg tgagtcctac gannnnnnnn nnnnnnnnnn nnnnnnngaa
     6361 cccgtccgga actatccaac tccacgtccc ctgccccatg ttgaagccct acccagactc
     6421 accacatagg acacggtcta agagggggct ccgtgggagc agacgagagg gcgatccgcg
     6481 acctggcccg gcttgccggc ttcgtgcact gggtacgaga ttggcccgag aagccgtcgt
     6541 ggcgcgaccg cctccgtcga tgggttaacc ccgagtcata cgactacacg ccatccatag
     6601 caccgccacc cagaccctgg gtcgacaggc cggccggaca ctacgaacgg caaggttggc
     6661 ggatacgcgc cgcatacttc ggtaccgaac tggccttcga ggtcgagtac gtggtctgta
     6721 aggcttgcga catggggtgg gtggaatccc cttacgccta tgaagggttc aagcgccgcg
     6781 gcctcgcctc agcagcactt tgcgctcttc gcgacacgta cccgggcctt gagtggcata
     6841 caggcggagg gcacttccgg gacgcagagc ccttctgggg tgcagtcagt gcaggcgtcc
     6901 acggcggata tacccagaga gctaggtgcc accacgtcga accgtggcaa gcgcggcaag
     6961 gatgaaagtg catgtaataa gcgacgcggc gtcatcccgc cccgagtgca gcgaacctct
     7021 aaggtcgctc gggctgatcc tggccaagcg ggaacgacgg gcggcggact gctggccagt
     7081 atgacggtgc tccgaccggc aaacccggtg gcgatgggca gaccggccgg atgatgtgtt
     7141 gaaggtctgc ccagctccgg acctgttccg ttgacatcca acggtgacat caacgaagcc
     7201 ggacggggac gcacaacagc accctggaac agtcgacgga tcaggccaca acgacaccta
     7261 ggacggggat agatcgaact cctaaagcgg gtgtcgcagg ttcgaatcct gccgggggca
     7321 ccaggcagaa ggccccgggc cgatcatgtc cggggccttc ggtgtttctg cgcgcccaag
     7381 tcggggccta ccccctcccc tcgatcagcg tcaccctgtt cccctccggg tcgcaaagag
     7441 cggcgaagcg gaccgtcccg tcggcgcctt cctgcggagc gtccgcctcc agcccccgct
     7501 cagccagctc ccccagcgcc ctgtccaggt ccgggacgcc gatgttgatc agggtcgagc
     7561 ccgcgtgctc cggcgagtgg aagagctgga gccaggtgtc cggggtgggc tgccattccg
     7621 tgagggaggg catggggtgg gtgtcggcgg ggcggccgag gaggtgggtg taccaggtgg
     7681 tggcgcgggc gaggtcggtg accggggcca cggcgaggag ggtggtgacg gggagcgggg
     7741 caccggcggt gggggtgttc atggaggtga tgggtaccgg ggtgccgggg ccgccaaaca
     7801 gccgcacgac accggagtgc cccgccgccg ccctgggcgg gcggggacgg ggcacggacg
     7861 gtgcggcgag gccggcgcct acgaagccac ccacccatgc acccgggcca gctcagtcac
     7921 ccgggtccgg atgcgcttga tctggtcggc ggtgagggtg tcgtcggtgt cgaggagaag
     7981 gtcggtgagt tcggtgcgga gttcggcgac ggtgagggtg tcggcctcgt cctggtcgtc
     8041 gtactcgtcg agttcggtgt cgggctcgtc gacgccgggg ttggtgatgc gcggggcgtc
     8101 ggggccgccg gaggagaagc cgacggcggg ggtggagcgg cggggggagg agcggtagac
     8161 gaggcggatc tgggaggcct tggggccctt ctcgcccatc tcctcgaaga actcgacctc
     8221 gcggccgctc tggtacaggt acttctcgtc gacaaggtcg ttggcgtgca tgaagacgtc
     8281 ctcgccgcct tcgttgggca cgatgaaccc gtagccgcgc acctcgtcga agcgcaggat
     8341 cttcccggtc ttcagcacaa caccacacct ccgctgtatc tcgcacgctg ccggtgaacc
     8401 gtttccggcg tgctgtgcgt ggccgtcccc tgggcgcggc cgtacccgtc tgcgccgcgt
     8461 ccgcctcggg cggcgcggct tcactcggcc ggatgatacg cgccccggtg cggccggggg
     8521 gagccgccgc gggttcccgt cacacggcca ggaccggccg ggagcggggg acggtacagt
     8581 cgcgggcggc cttcggccga gggtaccggg accggaacgg ggtggtgtgg gtgagcaaga
     8641 agcaggtccg cgagatgttc gagctgatgg ggcacgggca gccggtcgtg ttgacgagcc
     8701 cgatggcgtc ggtgaagaag ctggccgaac tggcctgcac ggcgcagctg ttcggctacg
     8761 agtacatcga cgtacggcag ggcggcgggc gtaacaccgc gctccacctg tacctcgcac
     8821 ccgatccgag ccccgcggcc cggcagcgtg cggcggccaa ctggcagcag tacccgcagg
     8881 cggccgacga cgggccgctg ccgccctacc cctcggacat gttcgagctg ctcaaggccc
     8941 gcatcaactt cgacctgacc ggccggctga cggagaccaa ccggctcgtg atggccgggc
     9001 tcgcgctcac ggtcgccgcc ggcctcatgc cgctgcggga cgtgccgacc gccttcgcgg
     9061 tgggcttctg ggtggtgacg atgggggcgc tcggcgtcgc cgtcctggtg cagcggcagc
     9121 ggaacgcgaa gttcgcggcg tacctggagg gttacggctt ccagcgggtc accgaccagg
     9181 agggccgcgc ccgctatctg ccgccggggg cgcataccgg ctggggcgcg ccggtgcagc
     9241 agcccggccc ttacgcccag ccgcagcagc cccagcccgc gccgtacggc tatccgcagc
     9301 cgggccggcc ccagcagccc gcgccctacg gctacccgca gcagccccag cagccgcagc
     9361 cgggcccgta cggccacccc cagcagggac agccgcagcc cccgcagccc ggccagtggc
     9421 agcatcagcc cggtcagtgg ccgcagcagg ccccgcagca gcccggccct cacgggcagc
     9481 cgccccagaa gtaggcgcct tcttccgcag gcggcccctt cgggctcttt cgggctgggc
     9541 cggaacccct ggcccgtccc cggtccgcct gccagcatcg gtgcatgagc aagcacttgg
     9601 gcccagaagc cggcccctcc cacgtcgacc cgtccctgat acgaccccgg cggggggtgc
     9661 tcgccggggt gtgggtggcc gcggggctgc tgctgctcgg cgcggtcgcc gggttcgtgc
     9721 tgacgctcgt gtcggccgtc gacgcgatcg accgcgagca ggcgttccgc agcggcggta
     9781 gtgcccgggt ggaggtgacg gccggcggcg agccggcggt gtacggacag gcgccggtgc
     9841 cggagggggc cgagtgcacg ctggacgggc ccggcgaggc gaagttcagc ccttacgggg
     9901 ccaggtacac cgtcaagctc aaccggacga cctgggtgcg gctcctcagg atcgaggccg
     9961 acaccccggg cacctacacc ctgcgctgca ccgacccggc gggctcggcc accttcgcgc
    10021 cgggtgacgg cgcgggactg ggtgccctcg ccaacacgct gctgcttcgg tccgcgctgc
    10081 ccggcctcgc ggggctcgcg ctggccggtg tcgccatcgc cctcatcgtc aagcgctccc
    10141 gtcaccgcaa ccgcctggcc gccgaggctc tgggccgctc gggcccgccc ccgcacggga
    10201 acggcccttc ccacggtcct ggtggccctg ccggtcccca gcacgacccg tggcagccgc
    10261 cgcccggggc cccgcgcaag gagtgaggga gtaaccgccg cccgccccgc tcaggccgac
    10321 cgcgccaccc cgtccccctc cgccacgtcg ccccccgccg ccagcgccgc cgcccgggcc
    10381 agttcgtcca gggagagccc cagcgtggtg gcgagggcgg cgaccgtgaa gaaggcgggg
    10441 gtcggggcgc ggcccgtctc gatcttgcgg agggtttcgg cggagacgcc ggcggctgcc
    10501 gcgacgtcga cca
//
