LOCUS       NZ_KZ195583            11329 bp    DNA     linear   CON 07-SEP-2024
DEFINITION  Streptomyces sp. CS227 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ195583
VERSION     NZ_KZ195583.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS227
  ORGANISM  Streptomyces sp. CS227
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6794013)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794013)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6794013)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 370.61x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188355.1-RS_2024_09_07
            Annotation Date                   :: 09/07/2024 02:15:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,017
            CDSs (total)                      :: 5,954
            Genes (coding)                    :: 5,785
            CDSs (with protein)               :: 5,785
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 72 of 169
            Pseudo Genes (incomplete)         :: 127 of 169
            Pseudo Genes (internal stop)      :: 12 of 169
            Pseudo Genes (multiple problems)  :: 41 of 169
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:26:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1089515
            Orig. end                         :: 1100844
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195583.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
FEATURES             Location/Qualifiers
     region          1..11329
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11329
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11329
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6329](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6329
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    211..226
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.75"
                     /tool="antismash"
     misc_feature    228..242
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     misc_feature    complement(425..440)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.41"
                     /tool="antismash"
     gene            complement(579..1340)
                     /locus_tag="B9W68_RS04690"
                     /old_locus_tag="B9W68_04700"
     CDS             complement(579..1340)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010641244.1"
                     /locus_tag="B9W68_RS04690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_04700"
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="WP_087772521.1"
                     /sec_met_domain="PEP_mutase (E-value: 2.5e-62, bitscore:
                     208.9, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFRTTVERAQRLQQLHADHQPLVLPTVWDVWSARTAADAGFPAL
                     TVGSHPLADSRGASDQEGQTFEEVLAAVRPIIAAVDVPVSVDLESGYGQDPADLVAGLT
                     EAGGVGLNIEDTVHSEGGRVRTTEEHARYIAGLRDAADAEGVPVWVNGRTDLFLHAENA
                     AAVLDEAVARLRAMEEAGADSVYPVRIQDDDELLQAVVAAVTVPVNSTAHPVKHDLDRF
                     RRLGVGRITYGPLLQMAATGAMKDMLRPWAP"
     gene            complement(1371..>1541)
                     /locus_tag="B9W68_RS04695"
                     /old_locus_tag="B9W68_04705"
                     /pseudo=""
     CDS             complement(1371..>1541)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951597.1"
                     /locus_tag="B9W68_RS04695"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_04705"
                     /product="RNA polymerase subunit sigma-24"
                     /pseudo=""
                     /transl_table=11
                     /translation="EHPVNGTPGLVGRRAGTVLTVAAFDLAADGRVARVWVVRNPEKLR
                     VWAGDDAPSPR"
     misc_feature    1528..1543
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.07"
                     /tool="antismash"
     gene            1740..3104
                     /locus_tag="B9W68_RS04700"
                     /old_locus_tag="B9W68_04710"
     CDS             1740..3104
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951595.1"
                     /locus_tag="B9W68_RS04700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_04710"
                     /product="PhoX family protein"
                     /protein_id="WP_030699547.1"
                     /transl_table=11
                     /translation="MSATRRQVLARTGAVAGIAFTGSLSELFAGTAAAKGMGKAGYGPL
                     VPDPKGLLDLPKGFRYRVLSREGDRMLSGEGQVPSNHDGMAAFGGRRGQVHLVRNHENR
                     PTAPIRVPPVRGLTYDPEGMGGCTALTLDPAHRVLGERVAIAGTSTNCAGGPTPWGTWL
                     TCEETEDKAGTNGYTKDHGFIFEVDPVDPHRTGAVPLKAMGRFQHEAIAIDPRHGIVYE
                     TEDAFERPFGLFYRFLPEKPRGGLGSLRAGGALQAMRVPGVPDLSAIQETGASFDRVEW
                     VDVPDPLAEETPIRFQDFGPKGITHAQKLEGCYWGGRSVYFVSSFARSSEGSAADHYGQ
                     IWRYDPDRRRLTLVIVFGPDTDVQLPGEEPDNICLAPSGGLMVAEDGEGAQHVYGVTTR
                     GEVYPMARGRQNIGTPEEPEWGEFAGVCFSPDYDTMFVNCYTPGTTFAVTGPWHR"
     gene            3274..4143
                     /locus_tag="B9W68_RS04705"
                     /old_locus_tag="B9W68_04715"
     CDS             3274..4143
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951592.1"
                     /locus_tag="B9W68_RS04705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_04715"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_087777747.1"
                     /transl_table=11
                     /translation="MVRKAKLRTGTALGALMAVGAVAGCGQSAPDPRDTAPGPRASHSA
                     SPAAPTAPPTLAPGPGGLAPVFANGPRNEGRTVALTFDADMTADQGERAAGGERFDHPE
                     LIASLRRLKVPATVFMTGRWAEEYPDQARAIGADPLFEIANHSYSHHAFTGDCYGLPRV
                     AESEMRADVERAYTAFRTAGVKKAMPYFRFPGGCYDDAALREVSAAGVTAVQWDVVSGD
                     AFATDADAVAQQVLDGVRPGSVVVMHCTRSAAPTTERALRRIVPALRERGYRFVKVSEL
                     IGAASRKG"
     gene            4175..4888
                     /locus_tag="B9W68_RS04710"
                     /old_locus_tag="B9W68_04720"
     CDS             4175..4888
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018894649.1"
                     /locus_tag="B9W68_RS04710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_04720"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_087772522.1"
                     /transl_table=11
                     /translation="MTSEPLSDSPFRDESTARDEAPQFVLPLVVRLERTEPPALTDALQ
                     TAARAVLALLSDPRSEGDGAWAKAVRDWQDARIRKVVRRARGGEWRKAAALPGITVDDG
                     GAEVRVYPPVPLDGWPKELARLQVSGTELEDPEEPGEPAPGVPVLWLNPDVRMSAGKAM
                     AQAGHGAQLAWWQLSGAERNAWREQGFPLAVRTAGAGRWKELVESGLPVVRDAGFTEIE
                     PGSCTVVAEHPALVG"
     gene            5001..6329
                     /locus_tag="B9W68_RS04715"
                     /old_locus_tag="B9W68_04725"
     CDS             5001..6329
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010641256.1"
                     /locus_tag="B9W68_RS04715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_04725"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_087772523.1"
                     /sec_met_domain="DUF692 (E-value: 7.5e-95, bitscore: 315.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPAIAEAVEAMAGIEWVEVVAENVCPGHLPDSLRR
                     LRERGVAVVPHGVGLGLGGAERPDPVRLAALAERAEALGSPLVTEHIAFVRAGGERTAS
                     RGLEAGHLLPVPRTREGLAVLCENVRIAQDQLPVPLAVENIAALFSWPGEELTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNQGEDPAAALDALPLEALAYVHVAGGFEREGVWHDSH
                     AHPVPEPVLAVLAELASRVRPPGVLLERDERFPSAAELEAEVEKIRRTVAAAGRGRVPV
                     GAGARPGSVPASGAGAAAGPVADEAARTRLALGQTALLSALVAGTPAPEGFDRGRLRVQ
                     SRMLTAKRGSVVASLAPELERILGDTYRPRFLRYAAARPLTGGYRADALAFALHTLREE
                     PPAERAARRALRDWWIERSGRASLVGRPWARWARVARFALLGR"
     misc_feature    6452..6470
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.23"
                     /tool="antismash"
     gene            6453..8093
                     /locus_tag="B9W68_RS04720"
                     /old_locus_tag="B9W68_04730"
     CDS             6453..8093
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (105-479). E-value:
                     1.6e-20. Score: 65.7. Matches aSDomain:
                     nrpspksdomains_B9W68_RS04720_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404759.1"
                     /locus_tag="B9W68_RS04720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_04730"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_087772524.1"
                     /sec_met_domain="PF00561 (E-value: 8.2e-24, bitscore: 83.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAPLILTAAGALLLTPLAAPPQAPAAGAPAPPGGPEARGVALAA
                     ERAARTPIAFGPCPDEEGLPRSLECGTVRVPLDYARPDGEQIDLTVSRAKAAGPQGERQ
                     GALVFNPGGPGSSGMLFPLVGIFPEWKRLASVYDLVGYAPRGVGRSAPLSCRDPREKPD
                     GSWQAPEHPAERQKAERRTKAEAYARGCAERGGKRLKHIHSLNNARDLDVVRAGLGERK
                     LTFMGASYGSYFGALYAKLFPSHVRRMVFDSAVDPSPSRVWYRNNMAQSAAFEQRWADF
                     RRWIASHDAAYGMGTDEAQVLAGYRRAREKLAAEPAGGAVGPAQLQAVMLRAQYYEDYW
                     PSHAEALAAYLKGDPQPLVNLATPLEEGRAEAENARAVYTAVECNDAPWPTSWETWDRD
                     HTRLARTAPFETWENAWMNLPCAYWKEPRQTPMDVRVAPGELPPTLILAGERDAATPYA
                     GALELRRRLPDAALVTERGAGTHGLAGGPNACVNRHVEAYLIDGKVPARSTDCAARPAP
                     EPEAREAGTRAGGSGPARPRVAGWSPAAR"
     aSDomain        6768..7889
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9W68_RS04720_Abhydrolase_1.1"
                     /evalue="1.60E-20"
                     /label="B9W68_RS04720_Abhydrolase_1.1"
                     /locus_tag="B9W68_RS04720"
                     /protein_end="479"
                     /protein_start="105"
                     /score="65.7"
                     /tool="antismash"
                     /translation="ALVFNPGGPGSSGMLFPLVGIFPEWKRLASVYDLVGYAPRGVGRS
                     APLSCRDPREKPDGSWQAPEHPAERQKAERRTKAEAYARGCAERGGKRLKHIHSLNNAR
                     DLDVVRAGLGERKLTFMGASYGSYFGALYAKLFPSHVRRMVFDSAVDPSPSRVWYRNNM
                     AQSAAFEQRWADFRRWIASHDAAYGMGTDEAQVLAGYRRAREKLAAEPAGGAVGPAQLQ
                     AVMLRAQYYEDYWPSHAEALAAYLKGDPQPLVNLATPLEEGRAEAENARAVYTAVECND
                     APWPTSWETWDRDHTRLARTAPFETWENAWMNLPCAYWKEPRQTPMDVRVAPGELPPTL
                     ILAGERDAATPYAGALELRRRLPDAALVTERGAG"
     gene            complement(8107..8823)
                     /gene="hemQ"
                     /locus_tag="B9W68_RS04725"
                     /old_locus_tag="B9W68_04735"
     CDS             complement(8107..8823)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951588.1"
                     /locus_tag="B9W68_RS04725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_04735"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_003951588.1"
                     /transl_table=11
                     /translation="MSAAPEKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPEDRSGYAD
                     EVQDLFDKLAAKDVTVRGTYDVSGLRADADVMIWWHAETADALQEAYNLFRRTRLGRAL
                     EPVWSNMALHRPAEFNKSHIPAFLADETPRDYISVYPFVRSYEWYLLPDEERRAMLADH
                     GKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARRHVREEVPF
                     YTGRRKSVADLVAQLA"
     gene            complement(8828..10300)
                     /gene="hemG"
                     /locus_tag="B9W68_RS04730"
                     /old_locus_tag="B9W68_04740"
     CDS             complement(8828..10300)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404755.1"
                     /locus_tag="B9W68_RS04730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_04740"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_087772525.1"
                     /sec_met_domain="DAO (E-value: 5.6e-09, bitscore: 34.4,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAETEGNPAGAGGVSPRVVVVGGGVAGLTAALRLLDAGAGVTLL
                     EASDRLGGKLRTGEIGGVRVDLGAESMLARRPEAVGLAREAGLADALQPPATASASLWT
                     RDALRPMPKGHVMGVPADPASLAGVLSEEGVARIAEDRTLPRTEVGEDIAVGEYVAARV
                     GREVVDRLVEPLLGGVYAGNAYRISLRAAVPQLFAAARDHTSLTEGVGALQARTAADPR
                     TDPVFMGIDGGVGRLPLAVADTVRARGGRIRTGTPVTGLARTPGGWRLTTGTPDAPEVL
                     EADAVVLAVPAPRAARLLAAELPAASADLAEIEYASMALMTFAFRADEAELPQGSGFLV
                     PPVDGRTVKAATFAGRKWRWIAEQQPGLHVVRTSVGRHREEEILGRDDAELLALARRDL
                     KAATGVDAEPVDAHVTRWDQGLPQYPVGHLARVARVRDQVAKAPGLALCGAAYDGVGIP
                     ACVASATEAARRVMAARPQGDPASGSSGHAGE"
ORIGIN
        1 gccggcgtgg ccctggccgc cacctcgaac accctgccgg gcaagctcgg cgagggccgt
       61 ttcgcggccg cggacttcct ccgcgagatc cagggcctct cggcgcactt ccgcccgctg
      121 aggatcgacg gcgaggacta ccgccaccgc ggcctgcccg ccgcgcccga gccgtacagc
      181 gacgagcagg tgacccgggc ggcgtacgcg acgccggggg cgagcctgga cgggttcggc
      241 cagctgctcg accacctgtc gcgggtccac cccagccggt acggggcgct caccgacgac
      301 ctgcgcgccg tctgcctcac cggcgtacgc ccggtgcccg accagtcgac ggccctgcgc
      361 ctggtggttc tggcggaccg gctgtacgac cgcgaggtgc ccgtgctgac ctcgggcgtg
      421 ccgttcgacc agctgttcag cgaggagatg ctgaacggcg gctaccggaa gaagtacttc
      481 cgggcgatat cccggctcac ggcgctggca cgggacggta agggactggt ggagcccggc
      541 gcggcgtagc cgggaggcgt gaccggccgg gcgtccggtc agggggccca gggccggagc
      601 atgtccttca tggcgccggt cgccgccatc tgtaggagcg ggccgtaggt gatgcggccg
      661 acgccgaggc ggcggaagcg gtccaggtcg tgcttgacgg ggtgggcggt ggagttgacg
      721 ggcacggtga cggcggcgac gaccgcctgg agcagctcgt cgtcgtcctg gatgcgcacc
      781 gggtagacgc tgtcggcacc cgcctcctcc atggcgcgca ggcgtgcgac cgcctcgtcg
      841 aggacggccg cggcgttctc ggcgtgcagg aagaggtcgg tgcggccgtt gacccagacc
      901 gggacgccct cggcgtcggc ggcgtcgcgc aggcccgcga tgtagcgggc gtgctcctcg
      961 gtggtgcgga cgcggccgcc ctccgagtgg accgtgtcct cgatgttgag gccgacgccg
     1021 cccgcctcgg tgaggccggc gacgaggtcg gcggggtcct ggccgtatcc ggactccagg
     1081 tcgacggaga ccgggacgtc gacggcggcg atgatcggcc ggacggcggc gagcacctcc
     1141 tcgaaggtct ggccctcctg gtcggaggcg cctcgggagt cggcgagggg gtggctgccg
     1201 acggtcagcg cggggaaccc ggcgtcggcc gccgtccgcg cggaccagac gtcccagacg
     1261 gtcggcagca cgagcggctg gtggtcggcg tgcagctgct ggaggcgctg agcgcgctcg
     1321 acggtggttc ggaagtccat gacggggaag ctacccccgg atcgccgcga tcaacgaggt
     1381 gacggcgcgt cgtcccccgc ccagacccgc agtttctccg ggttgcggac cacccagacg
     1441 cgggcgaccc ggccgtcggc ggcgaggtcg aaggcggcca cggtcagaac ggtccccgcg
     1501 cggcggccga ccaggcccgg cgtgccgttg accgggtgtt cggggcgagc ctgccggcgc
     1561 tggcccggcg gtgagggccg gggccgttcg gccgtacccg gtgtcagggc cgttttcggc
     1621 aggccgggtg ggctgtgacg gccgtgtcca gggccacgcc tggttccggt ggggctgggt
     1681 gcgtggtaca cacgtgcggg tcacattcct gtccgcagag ccgcagcagg gagttgccca
     1741 tgtccgcgac acgccgtcaa gtcctcgccc gtaccggtgc cgtcgccggt atcgccttca
     1801 ccggcagtct ctccgaactc ttcgcgggca cggccgccgc aaaggggatg gggaaggccg
     1861 ggtacggtcc gctggtgccc gacccgaagg gtctgctgga cctgccgaaa ggtttccgct
     1921 accgggtgct gtcccgggag ggcgaccgga tgctctccgg cgagggccag gtgccgagca
     1981 accacgacgg gatggccgcc ttcggtgggc gccggggcca ggtccacctc gtccgcaacc
     2041 acgagaaccg gcccaccgcc ccgatccgcg tcccgcccgt acgggggctg acgtacgacc
     2101 cggaggggat gggcggctgt accgcgctca ccctcgaccc cgcgcaccgc gtcctcggcg
     2161 agcgggtcgc catcgccggg acctcgacca actgcgccgg cgggccgacc ccctggggca
     2221 cctggctgac ctgcgaggag accgaggaca aggccgggac caacggctac accaaggacc
     2281 acggcttcat cttcgaggtc gacccggtcg acccgcacag gaccggcgcg gtgcccctga
     2341 aggcgatggg ccgcttccag cacgaggcca tcgcgatcga cccgcgccac gggatcgtct
     2401 acgagaccga ggacgccttc gagcggccgt tcgggctctt ctaccgcttc ctgcccgaga
     2461 agccgcgcgg cggcctcggc tcgctgcggg cagggggcgc gctccaggcg atgcgggtgc
     2521 ccggagtgcc cgacctctcg gcgatccagg agacgggcgc ctcgttcgac cgggtggagt
     2581 gggtggacgt gcccgacccg ctcgccgagg agacgccgat ccgcttccag gacttcggcc
     2641 cgaagggcat cacccacgcc cagaaactgg agggctgcta ctggggcggc cgctcggtct
     2701 acttcgtctc cagcttcgcc cggagcagcg agggctcggc cgccgaccac tacgggcaga
     2761 tctggcgcta cgacccggac cggcggcgcc tcaccctggt catcgtcttc ggccccgaca
     2821 ccgacgtgca gctcccgggc gaggagccgg acaacatctg cctcgccccg agcggcggcc
     2881 tgatggtcgc cgaggacggc gagggcgccc agcacgtcta cggcgtgacc acccgcggcg
     2941 aggtctaccc gatggcgcgg ggccgccaga acatcggcac ccccgaggag ccggagtggg
     3001 gcgagttcgc gggcgtctgc ttctccccgg actacgacac gatgttcgtc aactgctaca
     3061 cgccggggac gaccttcgcg gtgacggggc cctggcaccg gtgaggtgac cggggggcgg
     3121 gggcgggttg tcggtcccgc ccccgccccg cgggcccggt aaggcgcccg tacggcggag
     3181 ggcggcgggg cgagacgggt gaggccgcac ctcccgggcg gcgtttcgcc cgatattcag
     3241 cttttcgcat cctagggtca cggcgtgatc agcatggtgc gcaaggcgaa gctccggacc
     3301 ggtacggcgc tgggtgctct gatggcggtg ggtgcggtgg ccgggtgcgg ccagtccgcc
     3361 ccggaccccc gcgacacggc gccggggcca cgcgcctcgc actccgcctc ccccgccgcc
     3421 ccgacggcgc cgcccacgct ggcgcccggc cccggcggac tcgcccccgt cttcgccaac
     3481 gggccccgga acgagggcag gaccgtcgcg ctcaccttcg acgccgacat gacggccgac
     3541 cagggcgagc gcgcggccgg cggcgagcgc ttcgaccacc cggagctgat cgcctcgctg
     3601 cgccgcctga aggtgcccgc caccgtcttc atgaccgggc ggtgggccga ggagtacccc
     3661 gatcaggccc gggccatcgg cgccgacccg ctcttcgaga tcgccaacca ctcctacagc
     3721 caccacgcct tcaccggtga ctgctacggc ctgccgcgcg tcgccgagag cgagatgcgg
     3781 gccgacgtgg aacgggcgta caccgcgttc cgcacggccg gggtgaagaa ggcgatgccg
     3841 tacttccgct tccccggcgg gtgttacgac gacgcggcgc tgcgggaggt ctcggcggcc
     3901 ggggtgacgg ccgtgcagtg ggacgtggtg agcggggacg ccttcgccac cgacgccgac
     3961 gcggtggcgc agcaggtgct ggacggggtc cgccccggct cggtcgtggt catgcactgc
     4021 acccgcagcg ccgcccccac caccgagcgc gcgctgcgcc ggatcgtgcc cgcgctgcgc
     4081 gagcggggat accgcttcgt caaggtgtcc gagctgatcg gggcggcctc gcgaaagggg
     4141 tgagcaaggt ctggcccttt ggcagactgg ctccgtgacc agcgaacctc tctccgacag
     4201 tcccttccgt gacgaaagca ccgcccgcga cgaggcaccg cagttcgtgc tgccgctcgt
     4261 cgtccggctg gagcgtaccg agccgcccgc cctcacagac gcgctccaga cggccgcccg
     4321 ggccgtcctg gcgctgctca gcgacccccg ctccgagggg gacggcgcat gggccaaggc
     4381 ggtacgggac tggcaggacg cgcgcatccg caaggtggtg cggcgggccc gcggcggcga
     4441 gtggcgcaag gccgccgccc tgccggggat cacggtggac gacggcggcg ccgaggtgcg
     4501 ggtctacccg ccggtcccgc tggacggctg gccgaaggaa ctggccaggc tccaggtctc
     4561 cggcaccgag ctggaggacc ccgaggagcc gggcgagccc gcgcccggcg tcccggtgct
     4621 ctggctcaac ccggacgtgc gcatgtcggc ggggaaggcg atggcccagg cagggcacgg
     4681 cgcgcagctg gcctggtggc agctgtcggg ggcggaacgg aatgcctggc gcgaacaggg
     4741 tttcccgctg gcggtgcgga ccgccggcgc cgggcgctgg aaggaactgg tggagagcgg
     4801 gctgccggtg gtgcgggacg cggggttcac ggagatcgag cccgggtcgt gcacggtggt
     4861 ggcggagcat cccgcgttgg tggggtgagg ggcggcgaag ctcaaatcct gctctgaaga
     4921 gggcgggtgc cggttccggg gtggacggtg gcgggcaggg tggccgcagg ggccgggggg
     4981 ttcctggagg gggtggcggt atggaacggc tgggtacggg gatcggctgg cggcccgcga
     5041 tcgcggaggc cgtcgaggcg atggcgggga tcgagtgggt ggaggtggtg gcggagaacg
     5101 tctgtcccgg tcacctaccc gactccctgc ggcggctgcg ggagcgtggg gtggccgtgg
     5161 tgccgcacgg ggtggggctg ggactgggcg gcgcggagcg gcccgatccg gtacggctgg
     5221 cggcactggc cgagcgggcc gaggcgctgg ggtcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtccgggc gggcggggag cggaccgcgt ctcgcgggct ggaggccggg cacctgctgc
     5341 cggtcccccg gacccgggaa gggctcgcgg tcctctgcga gaacgtgcgg atcgcccagg
     5401 accagctccc cgtgccgctc gccgtggaga acatcgcggc gctcttctcc tggccgggcg
     5461 aggagctgac ggaggggcag ttcctctacg agctggccga ccgcaccggg gtacggctgc
     5521 tgatcgacgt cgccaacctg cacaccaacc acgtcaacca gggcgaggac cccgccgccg
     5581 ccctcgacgc gctgccgctg gaggcgctgg cctacgtcca cgtggcgggc gggttcgagc
     5641 gggagggggt gtggcacgac agccacgccc acccggtgcc ggagccggtg ctcgcggtcc
     5701 tggccgagct ggcctcgcgg gtgcggccgc ccggggtgct gctggagcgg gacgagcggt
     5761 tcccgtcggc ggccgagctg gaggcggagg tcgagaagat ccggcggacg gtggcggcgg
     5821 ccggccgggg ccgggtcccg gtgggggcgg gggcgcggcc cgggtccgta ccggcgtcgg
     5881 gggccggggc ggcggccggg ccggtggcgg acgaggcggc gcggacgcgg ctggccctcg
     5941 ggcagacggc gctgttgtcg gcgctggtcg cggggactcc ggcgccggag gggttcgacc
     6001 gggggcggct gcgggtgcag agccggatgc tgacggccaa gcgggggtcc gtcgtggcct
     6061 ccctcgcgcc ggagctggag cggatcctcg gcgacacgta ccggccgcgc ttcctgcggt
     6121 acgcggcggc ccggccgctg accggcgggt accgcgcgga cgcgctcgcc ttcgcgctgc
     6181 acaccctgcg ggaggaaccg ccggccgagc gggcggcccg gcgcgcgctg cgcgactggt
     6241 ggatcgaacg ctcgggccgc gcgtccctgg tgggcagacc gtgggcccgg tgggcgcggg
     6301 tggcgcgctt cgcgctgctg ggacgctgag gccgcgggcc gtgttgggcc gatccgagcg
     6361 ggtccgtcca acacgcggcc cggtgcttgc ccttgccgat cggcctgcga atcatccctt
     6421 gtgccgttca tcggccggac gaaagggacg cgatgcgagc gccgctcatc ctcaccgccg
     6481 ccggagccct gctcctcacc ccgctcgcgg ctcccccgca ggctccggcg gccggtgctc
     6541 cggctccgcc cggtggcccc gaggcgcgcg gggtggcgct ggcggccgag cgggcggcgc
     6601 ggaccccgat cgccttcggg ccctgtcccg acgaggaggg gctgccccgg tcgctggagt
     6661 gcggcacggt acgggtcccg ctggactacg cgcggccgga cggcgagcag atcgacctga
     6721 cggtgagccg ggccaaggcc gcgggtccgc agggcgagcg gcagggcgcg ctggtcttca
     6781 acccgggcgg tcccggctcc tcggggatgc tcttcccgct ggtggggatc tttcccgagt
     6841 ggaagcggct ggcctccgtc tacgacctgg tcggctacgc cccgcgcggg gtcggccgtt
     6901 cggcgccgct ctcctgccgg gacccgcgcg agaagccgga cgggagctgg caggcccccg
     6961 agcacccggc cgagaggcag aaggccgagc ggaggaccaa ggccgaggcg tacgcgcggg
     7021 ggtgtgccga gcgcggcggg aagcggctga agcacatcca ctcactgaac aacgcccgcg
     7081 acctggacgt ggtgcgggcc ggcctcggtg agcggaagct gacgttcatg ggcgcctcgt
     7141 acggctcgta cttcggcgcg ctgtacgcga agctcttccc ctcgcacgtg cgcaggatgg
     7201 tcttcgactc ggcggtcgac ccctcaccct cgcgcgtctg gtaccggaac aacatggcgc
     7261 agtcggcggc gttcgagcag cggtgggcgg acttccggcg gtggatcgcc tcgcacgacg
     7321 cggcctacgg gatgggtacc gacgaggccc aggtgctggc ggggtaccgg cgggcgcgcg
     7381 agaagctggc ggccgagccc gccgggggcg cggtcggccc ggcgcagctc caggcggtga
     7441 tgttgcgggc ccagtactac gaggactact ggccctcgca cgcggaggcg ctcgcggcgt
     7501 acctgaaggg tgatccgcag ccgctggtca atctggcgac cccgctggag gagggccggg
     7561 cggaggcgga gaacgcgcgg gccgtctaca cggcggtgga gtgcaacgac gcgccgtggc
     7621 ccaccagctg ggagacctgg gaccgcgacc acacccggct cgcccgcacg gcgccgttcg
     7681 agacctggga gaacgcctgg atgaacctgc cgtgcgccta ctggaaggag ccccggcaga
     7741 cgccgatgga cgtgcgggtg gcccccggtg agctgccgcc gacgctgatc ctcgccgggg
     7801 aacgggacgc ggcgaccccg tacgcgggcg cgctggagct gcggcggcgg ctgccggacg
     7861 ccgcgctggt gaccgagcgg ggcgcgggca cgcacgggct ggcgggcggg ccgaacgcct
     7921 gcgtcaaccg gcacgtggag gcctacctga tcgacgggaa ggttcccgcg cgctccaccg
     7981 actgcgcggc ccggccggcc cccgagccgg aggcgcgtga ggccgggacg cgggcgggcg
     8041 ggagcggacc ggcgcgcccc cgggtcgccg ggtggtcccc ggcggcccgg tgaggcggag
     8101 cggggctcag gcgagctggg cgaccaggtc ggcgacggac ttgcggcgcc cggtgtagaa
     8161 gggcacctcc tcgcggacgt ggcggcgggc ctcggaggcg cgcaggtggc gcatgaggtc
     8221 gacgatgcgg tacagctcgt cggcctcgaa ggcgagaatc cactcgtagt cgccgaggga
     8281 gaacgaggcg accgtgttgg cgcggacgtc cgggtagccg cgcgccatct tgccgtggtc
     8341 ggcgagcatc gcccggcgct cctcgtcggg gaggaggtac cactcgtagg agcggacgaa
     8401 ggggtagacg ctgatgtagt cgcggggggt ctcgtcggcg aggaacgccg ggatgtgcga
     8461 cttgttgaac tcggcggggc ggtgcagcgc catgttcgac cagaccggct ccagcgcgcg
     8521 cccgaggcgg gtgcggcgga agaggttgta cgcctcctgg agggcgtcgg cggtctcggc
     8581 gtgccaccag atcatcacgt cggcgtcggc gcgcagaccg gagacgtcgt aggtgccgcg
     8641 gacggtgacg tccttggcgg cgagcttgtc gaacaggtcc tggacctcgt cggcgtagcc
     8701 ggaccggtcc tcggggagca cgtcgcggag cttgaagacg gaccacaggg tgtagcggat
     8761 gacctcgttg aggtccttgg ccttcttgcc cgcgttgggg atcttctcgg gagcagcact
     8821 catggggtca ttctcccgcg tgcccggacg agcccgacgc cgggtcgccc tgtgggcggg
     8881 ccgccatgac ccggcgcgcc gcttcggtgg cggaggcgac gcaggcgggg atgccgacgc
     8941 cgtcgtaggc ggcgccgcac agggcgaggc cgggggcctt ggcgacctgg tcgcggacgc
     9001 gggcgacgcg ggcgaggtgg ccgaccgggt actggggcag gccctggtcc cagcgggtga
     9061 cgtgggcgtc gaccggctcg gcgtcgacgc cggtggcggc cttgaggtcg cggcgggcga
     9121 gggcgaggag ttcggcgtcg tcgcggccga ggatctcctc ctcgcggtgg cggccgaccg
     9181 aggtgcgcac gacgtgcagc cccggctgct gctcggcgat ccaccgccac ttgcgtcccg
     9241 cgaaggtggc ggccttgacg gtgcgtccgt cgaccggcgg gacgaggaag ccgctgccct
     9301 gcggcagctc ggcctcgtcc gcgcggaagg cgaaggtcat cagggccatc gaggcgtact
     9361 cgatctcggc gaggtcggcg gaggcggccg ggagttcggc ggcgagcagc cgggcggccc
     9421 ggggcgcggg gacggcgagg accacggcgt cggcctccag gacctcgggc gcgtcggggg
     9481 tgccggtggt cagccgccag ccgcccgggg tgcgggcgag gccggtgacg ggcgtaccgg
     9541 tgcggatgcg gccgccgcgg gcgcggacgg tgtcggcgac ggcgagcggg aggcggccga
     9601 ccccgccgtc gatgcccatg aagaccgggt cggtgcgcgg gtcggcggcg gtgcgggcct
     9661 ggagcgcacc gacgccctcg gtcagggagg tgtggtcgcg ggcggcggcg aagagctggg
     9721 ggacggcggc gcgcagcgag atgcggtagg cgttgccggc gtagacgccg ccgaggaggg
     9781 gttcgacgag ccggtcgacg acctcgcggc cgacccgggc ggcgacgtac tcgccgacgg
     9841 cgatgtcctc gccgacctcg gtgcggggca gggtgcggtc ctcggcgata cgcgccacgc
     9901 cctcctcgga gaggaccccg gcgaggctgg ccgggtcggc ggggacgccc atgacgtggc
     9961 ccttgggcat ggggcgcagg gcgtcgcggg tccacaggga ggcgctggcg gtggcggggg
    10021 gctggagggc gtcggccagt cccgcctcac gggcgaggcc gaccgcctcg gggcgtcggg
    10081 ccagcatcga ctcggcgccc aggtcgacgc ggacgccgcc gatctcgccg gtacggagct
    10141 tgccgccgag ccggtcggag gcttccagga gggtgacgcc ggccccggcg tcgagcaggc
    10201 gcagcgcggc cgtgagcccc gcgacgccgc ctccgacgac caccacacgg gggctcacgc
    10261 cacccgctcc cgccgggttt ccctctgttt ccgcgcgcat ggagccactg tctcaaaccg
    10321 gacggggcgg gcgggcgccg gggcggcgcg gttccgttat ccgttcgcga cccgggaggc
    10381 gaaacccacc ggtgcgccgg gccgtcgtag cggcacgggc gcctcggtcg gggcgcccga
    10441 aggacacaag ggggcggcgg atgagccgga tgcggggaac gagcggcggc cggatcgtgg
    10501 ggcggcgggc ggcggtggtg ctggcggtgg cggtactggg tctcgcgggg tgttcggccg
    10561 ggtcggactc cggggcgtcg tcggcggggg accgtgcggc cgcgccgtcg gcggccgacg
    10621 gcgcgggcgg cggtcaggcc ggcaacgcga agcgggagtc ggcggacgag gccgcggacg
    10681 ggaaggcgcg ccccgagctg aagggcgtcc acctgatccg tacggccgaa ctgcacctgc
    10741 gggtgaagga cgtgccctcc gcgctggcct cggcccggtc ggtggtcgag ggcgcgggag
    10801 gctacgtcgg caacgagtcg acccggcgcg actcccagga ccacgagcgg tcgaccgtgg
    10861 tggtccgcgt cccgcaggag cggtacgagg cggtgctgga cgcgctcggc gagggcggcg
    10921 aggtggtgtc ccggcggacc agcgcggagg acgtcaccga ggaggtggtg gacgtcgaga
    10981 gccggctgaa gtcgcagcgg gccagtgtgg cgcgggtccg ggagctgatg gaccgggcgg
    11041 agaagctgag cgacatcgtc tcgctggagg gcgagctgag cagccgccag atggaactgg
    11101 aggcgctgct ggcccagcag tcctcgctga aggaccgcac ctcgatggcg acggtcaccc
    11161 tccagctgac cgagcggtcg gcgaagacgg agtcgaagga ggagggcggc ccgaccttca
    11221 ccgacgcgct ctccggcggc tggtcggcgt tcgtggcgac gctgcgctgg ctgaccgtgg
    11281 tggtcggggc ggcgctgccc ttcgtcctcg ccctcgggct ggccctgct
//
