LOCUS       NZ_KZ195583            10216 bp    DNA     linear   CON 07-SEP-2024
DEFINITION  Streptomyces sp. CS227 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ195583
VERSION     NZ_KZ195583.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS227
  ORGANISM  Streptomyces sp. CS227
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6794013)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794013)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6794013)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 370.61x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188355.1-RS_2024_09_07
            Annotation Date                   :: 09/07/2024 02:15:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,017
            CDSs (total)                      :: 5,954
            Genes (coding)                    :: 5,785
            CDSs (with protein)               :: 5,785
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 72 of 169
            Pseudo Genes (incomplete)         :: 127 of 169
            Pseudo Genes (internal stop)      :: 12 of 169
            Pseudo Genes (multiple problems)  :: 41 of 169
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:26:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 694123
            Orig. end                         :: 704339
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195583.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5216
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    45..58
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.11"
                     /tool="antismash"
     gene            complement(3155..3559)
                     /locus_tag="B9W68_RS02900"
                     /old_locus_tag="B9W68_02915"
     CDS             complement(3155..3559)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010636353.1"
                     /locus_tag="B9W68_RS02900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_02915"
                     /product="CU044_2847 family protein"
                     /protein_id="WP_256967938.1"
                     /transl_table=11
                     /translation="MAYLVELPMSGAGGTGGDGRGVASGPADAGGVVKVEIDEVADGLV
                     QVARPGQVVARATRPLKEMLAGIRPVAENFVEGFRDMAQRPDEIVVEFGLSVSAEADVV
                     ISRTSGQANFQVTLTWSSPPSGDPAADTAV"
     gene            3819..4325
                     /locus_tag="B9W68_RS02905"
                     /old_locus_tag="B9W68_02920"
     CDS             3819..4325
                     /EC_number="2.3.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640757.1"
                     /locus_tag="B9W68_RS02905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_02920"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_087772258.1"
                     /transl_table=11
                     /translation="MRIRSVHRAELTALQEIERAAGVWFRDLGMAEIAEDEPPSVAELA
                     RYERAGLAWVAADEADRPVAYLVAERVDGNLHVEQVTVRPESARRGVGRALLDHAADRA
                     RSEGAAALTLTTFTEVPWNAPYYVRCGFRFLAEGELTAGLREIRRAEAAHGLDRWPRCC
                     MRREV"
     misc_feature    complement(4515..4532)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.61"
                     /tool="antismash"
     gene            5001..5216
                     /locus_tag="B9W68_RS02910"
                     /old_locus_tag="B9W68_02925"
     CDS             5001..5216
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="B9W68_RS02910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_02925"
                     /product="hypothetical protein"
                     /protein_id="WP_008412458.1"
                     /sec_met_domain="strepbact (E-value: 4.1e-37, bitscore:
                     125.0, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYTPQVETAEIADSELDNISGGLVNGLVGSVTSSLDSVAPVSDT
                     LGTVTGVVEQTTGLNTAPVTGLVAGL"
     misc_feature    5015..5030
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.07"
                     /tool="antismash"
     misc_feature    5388..5403
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.77"
                     /tool="antismash"
     gene            5445..6251
                     /locus_tag="B9W68_RS02915"
                     /old_locus_tag="B9W68_02930"
     CDS             5445..6251
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640761.1"
                     /locus_tag="B9W68_RS02915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_02930"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_087772259.1"
                     /transl_table=11
                     /translation="MRFRQQALTKLQSPEQLDLPVRFARPQGLLVLAVTLVVMAAGGVW
                     AVTGSVATTLTAPGVLTHAQGSYVVQSTVSGQVTAVRTAEGHRVARGTPLLTVRTARGD
                     VTIRALAAGRVTALAAATGAVVTTGSDLAAVERQENPDDPLLAVLYAPADSGASLRPGA
                     KVDLTVQSVPAQSYGVLRGTVKSVGRTARTQQQISADLGSTQLGEEFSRNGPPIAVEVR
                     LHPTKSTRSGYTWSTSDGPPHPLTTLTLVDGAVHLDEQRPADWLLP"
     gene            6248..8545
                     /locus_tag="B9W68_RS02920"
                     /old_locus_tag="B9W68_02935"
     CDS             6248..8545
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640763.1"
                     /locus_tag="B9W68_RS02920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W68_02935"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_087772260.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 8.7e-36, bitscore:
                     121.1, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPADAPAAGTGSTAPPTPGGRRGRRAVPSSGRRRAAPPRRTPR
                     RRPVRTPTVLQMEAVECGAACLAMVLAHHGRHVPLEELRIACGVSRDGSRASNVLKAAR
                     SYGLTAKGMQMEPAALREVRAPAVLFWEFNHYVVHEGFLRRPGRRGAVRLNDPDKGRRV
                     VSAEEFDASFTGVALVLEPGSSFRKGGRKPGLLGALPARLRGTGATLLTALLASLLLVV
                     VGAALPALSRTYIDLFLIGERTSVLGPLFAAMGALVALTASLTALQQANLLRGRIISST
                     LSSARFLRHLLRLPVTFFAQRSPADLVQRLQSNDTVAETLARDLAAAAVDGVVVVLYAL
                     LLWTYDPQLTLVGVGIALLNIVAMRVVIQLRATRTQKLRADNARLTTTAYTGLQLIETM
                     KATGGEDGFFRRWAGQHATTLEEQQRLGVPTAVLSVVAPALATFNSALILWIGGLRAVE
                     GHLSVGLLVAFQALMVRFTAPLTRLNGVAGRIQDFAADVTRLRDVEAFPADGLYEQEGG
                     GSGEADTRRLAGRVELEGLTFGYSPLDAPLLTEFSLTVGPGRQVALVGGSGSGKSTVSR
                     LLAGLYQPWEGTIRIDGQPLTDIPRSALAASVSFVDQDIFLFEGTVRENVTLWDPSLPD
                     EAVRTALRDAGLEEVIARRPDGIHARVEQDGRNFSGGQRQRLEIARALVRRPSVLVLDE
                     VTSALDAETEQRVMAALRRRGCACVIIAHRLSTVRDSDEIVVLDGGRVVERGRHAELVA
                     RGGAYAALVEEK"
ORIGIN
        1 gtggaaacgc ccccgggcag gggcgctcgg gagatggcct cgcctcggtc cagatcgagg
       61 acacgcactt cgtcgcccca ctggagcacg acgcgccgcc cggcctcgtc gaccgcttgc
      121 accgtgcaca tgtccgtggt gtacgcctcg tcccgctcgc ggtaggagcg gccggtctcg
      181 gagccgtcgg aggtccggta gcggcggatg acggactgcc cttgctcctc gcggcaggag
      241 acggcggtcc tgcggtcgcc ggagaggtag atctcctgct tcccggagag aagccgcgtg
      301 gcacggcccg tggtcaggtc gacggagttc aggagccggg tgcccccttc cccgtaggac
      361 gtcgccagga gcgtccgccc gtccggggcg aaggcgagcg ggcccgtcag gtccgggggc
      421 gcgggggcgt caccggtgcg tgtgccgctc tccaggttcc accgcaccag ccggtcgccg
      481 acgaccgagg ctaggctgcg cccgtccgcc gacatcgcgg gaatcacgtc aggatcgaga
      541 cgcacgtccg gcccgtcgct cgccggccgc ttcggcccga gcaggcccgc ggcatcgctg
      601 accacgtcga accggaccgc cttgccgtcc gcctggacgt aggcgatgcg cttcccgtcc
      661 gcggcgacca cgccgtgcat gacctgcccg gtggagggca cctgtgcgct gcgtacccgc
      721 ccggaggcga cgccggtgaa caggatggcc cggccgagct gcgagacggc gaggaccacg
      781 tcgccgtccg cgctggtctc gaactccctg acggtgccga ggacgccgga gagcacccgg
      841 tcgtacccct ggtaccgcag gtactgcctc aggagttcgt cgcgcgcctc ctgggtcggt
      901 gcggtccggt gcgcggccag ggctttcagc acgcccaggg cgggtgcggt gcgcgtctcg
      961 tccgccgcgg ccagagccag cgcgcgggac gcggcctccg cggcacgttt ccggctctcc
     1021 tcgcgtgtgg cgtggaacgc cgccagggcc acgatgacca ggagcacgac gaagctgatc
     1081 gccgagagcg tcacccgtct ccggcgggcc ctcgctcggc gtcgggtgtg ggcggcctcc
     1141 agatagcggc gttcggcgtc ggtgagttcg tcgccgcgtg cgtccagcca ctcttcggac
     1201 cgggcgagtt gcagcggcgt cggcagcagt tccgctgggc gttcggcctg ttcccagcgg
     1261 tccatgtccc ttcgcagccc agcgcgccac tccaggaagg cacggtcacc ggtggcccag
     1321 tcggagagct tgtcccaccc ggtgatcagg gcctcgtggg cgagttccac ggtctcggtg
     1381 ccctcggcgt tccggccggt caccaacagc cgtgtgcccg ccaggcgttg cgcgacacgc
     1441 cactcccctg ctcccagctc ggtgcgcagt gccgtccgcc gggcgggcgc gggcagatcg
     1501 gcggtgacct ggacgagtcg ggtgaacagc cgtctggcga cctcctcgtc ctccgggggc
     1561 acgcacgcgc tccacccgtc gtcggcgtgc cggcccaggg caccgctgac gccgccgagt
     1621 cgctcgtagg cgcggtgagt cagcaggccg ccctcctgcc gctgccacaa caggtcgagg
     1681 gtgaggccga ggagcggcag ctcgcccggt cccgcgccgg tgtcggcgat gacgcgctcg
     1741 gcgagctgag gctcgtagga caccccgggt acggcgtcga cgggtgcggt cacgatctcg
     1801 cgcaggcgcc cggggtccgg cggggcgagc gcgtacaggt gctcgccggc caccgggccc
     1861 aggcgcggat cggccagcac cggctccagg aagtcggcgc gcagcgtcat cagcacgcgg
     1921 acggtgtccg gcagggtgcc gcccgtgagc aggtcggcga attcgtcggc tccgggcggg
     1981 tcgaggccga gggcctcctc gaactggtcg accaccagca gcagtcggct gccgtgtcgc
     2041 agctggagca gccgtgacac cgtgtccgcc gggccgttgc ggcgcagcac agcggcgagt
     2101 tcggccgtcc gctcctggcg ctcggtctcg gccaggccgg gttccagcag cggcagcagt
     2161 cctgccgcga gggaagcgaa cggggcgctg ccgcgcgtcg gccgcaccac cacgacctgg
     2221 gcgccctggg cgcgcaagag gggcaggact ccggccaggg ccagcgacga cttgcccgag
     2281 cccgacggtc ccacgacggt gacacggcgc tcggtggaca gcgcccgcgc cacctcctcg
     2341 ctctccgcct gccggccgtg gaagagcgct gcgtcggcct cctcgaacgc cgccaggccc
     2401 cggaacggcg acggcggcag cgcgaccgcg cgcaactggg gacagactgc cagcaggccg
     2461 ctcgtcggga ccaggtagct gacaggcggc tctcccacct cagccaccgt caccatgccg
     2521 accacacccg agagctggtc gtcccagacc gggccgccgc tgaaaccccg cgagaccgga
     2581 tagccctctc cggcgagatc ggcctgcacc cagccgtccg cctgccgccc gcgcagcacg
     2641 cccgcgtgcc agaccccacc cgggcgcccg gcgggcagcc cgtacgctcg caacgggtgc
     2701 ccccacacct cgtccgcctc caccagccgc accggtcccg cccccggaag cggtgcctcc
     2761 agccgcagca gggcgacatc ggcgggcacg acgaccccgt cacggccggg ggtggactcc
     2821 gtacgggcgg cgggcaccgg tggcacctcg gcgggcgccc gcggcggcac ccactgttcg
     2881 acacgggtcg tcgtcaccgg cgtgtcgccg tcgcgctgcg gggctggcac cagaggcagg
     2941 tcgacctgga cccgggcaaa ggcatcaggc gccgcgtccc ccgtcgcccc cagggctgcc
     3001 tggaccacgt gcgcgcaggt caaggcgagg gtgtcggaga ccaggaaggc gacaccgacc
     3061 ccctgcccat ggctgtcgcg gacccgcagg acggccgcgt ccagcgaccg gggtcccggg
     3121 accaccgggt gcgcgggtgg cgacccgggc accctcagac cgccgtgtcg gcagcggggt
     3181 cgccggaggg cgggctggac caggtcaggg tgacctggaa gttggcctgg ccgctcgtcc
     3241 gggagatcac cacgtcagcc tcggccgaca cggagagacc gaactcgacg acgatctcgt
     3301 cgggtcgctg cgccatgtca cggaacccct ccacgaagtt ctccgcgacc ggcctgatcc
     3361 cggcgagcat ctccttcagc ggccgggtag cccgcgccac cacctgcccg ggacgcgcga
     3421 cctgcaccag cccgtccgcg acctcgtcga tctcgacctt gacgacaccg cccgcgtccg
     3481 ccggaccgga cgcgaccccc cgtccatccc ctccggtgcc accggccccg gacatgggca
     3541 actcgaccag atacgccatg cttccccctt cacgacacct gccacgctgg cccagcaccc
     3601 taccgacggc gacggcgccg cgaggctcga acgacgcgaa acgccgtcgt accgacgtga
     3661 tctgaccgga cgtcggcgaa cgaacggggt gtgccggcgt cccgtggctc gcggtcccgg
     3721 gacggtgctc gcaggttcga gtccgggtcg tccgttgccg ggagggggcg cccccgtttc
     3781 tcgttgcttc ccgccgccgc gcaccggaca atgcccccat gcgcatccga tccgtgcatc
     3841 gcgccgaact gacggccctc caggagatcg agcgagccgc cggagtctgg ttccgcgatc
     3901 tcggcatggc cgagatcgcg gaggacgagc cgccttcggt ggcggagctg gcgcggtacg
     3961 aacgggcggg gctcgcctgg gtcgcggccg acgaggcgga ccggcccgtg gcgtacctgg
     4021 tcgccgagcg ggtcgacgga aacctccacg tcgagcaggt gacggtgcgt ccggagagtg
     4081 cgcgccgggg tgtcggccgg gccctgctgg accatgccgc cgaccgggcc cggagcgagg
     4141 gggccgcggc gctgacgctc accaccttca ccgaggtgcc gtggaacgcg ccgtactacg
     4201 tgcggtgcgg cttccgcttt ctggcggagg gtgagctgac ggctgggctg cgggagatcc
     4261 ggcgggccga ggctgcgcac ggactggacc ggtggccccg ctgctgcatg cgccgggagg
     4321 tgtgagcgcg gtcgccggag agagtccggc ctctccgccg gggaatgcac gccccccggg
     4381 cccctctttt catggccctg ccggtattcc cgcccctggc tctccgcccg tttttcgcag
     4441 tgcgcatgaa tgcgccgggc gatggcaatt acgccacttc cactccttct cctcctcgcg
     4501 gcggacgcta ttcctccaca tgttggaaac atggccttct cagagccgtc cgcacacgaa
     4561 tccgccgcgc cccggggcgg gttctcctgc ggtgaatctg cacagccgtg ccgcagctcc
     4621 gtcaccgtca cccatacctt caccccgtgt gaacggccga ggtcgacggc tcgcaccagg
     4681 gcttcacagc aaggggcgtg gtgtacacgc gggcgtgacc ccaccccgca cggcggcggg
     4741 aaggtgtgaa ggtggtgccg ttctcctgtg ccggattccg cacacggcgg tgaggagaag
     4801 ggcaccgccc gccgtattcc gctctcaccg attggcagcc ccgcgcaccg ccgtgctttg
     4861 atccatcgca ctgcggcacc gcccacggtt ctgaaggctc aacgcctttc gcccgggggc
     4921 cgccagagga tcttcccctt cttctgcacg agtgcgccgc ggtgaccatt tccgtcagtg
     4981 atgcgaaggg aactcccccc atgagctaca ccccccaggt cgagaccgcc gagatcgccg
     5041 acagcgagct ggacaacatc tccggcggcc tcgtgaacgg cctcgtcggc tcggtcacct
     5101 ccagcctcga ctccgtcgcc cccgtctccg acaccctcgg caccgtcacc ggcgtcgtgg
     5161 agcagaccac cggtctgaac accgccccgg tcaccggcct cgtcgccggt ctctgagacc
     5221 ccacgcgtcg cctgacgacc gtgcggcccg ctgaggcgga ccgctcggcc ggtgccccgg
     5281 acccgctcgg cttccggggc accggccgcc tccttcgtcc tccccggacg cccgcgcggc
     5341 gtccccagca ccacggcgac atccggccgc gcggcctggg gatgccttcg aagtcctgtg
     5401 cggtgtccgc ggtggccggt gcccggcagt cgaggaagtc ttccgtgcgc tttcgtcaac
     5461 aggccctgac caagctccag tcgcccgaac agctggacct gccggtgcgg ttcgcccgcc
     5521 cgcaggggct tctggtcctc gccgtcaccc tcgtcgtgat ggcggccggc ggcgtgtggg
     5581 ccgtcaccgg ttcggtcgcc accaccctca ccgcgcccgg cgtcctcacc cacgcccagg
     5641 gcagctacgt cgtccagtcc accgtctccg gccaggtcac cgccgtccgc accgccgagg
     5701 gccaccgcgt cgcccgcggc accccgctgc tgaccgtccg caccgcccgg ggcgacgtga
     5761 ccatccgcgc cctggccgcg gggcgggtca ccgcgctggc cgccgccacc ggcgccgtgg
     5821 tcaccaccgg ctccgacctg gccgccgtcg agcgccagga gaacccggac gacccgctcc
     5881 tcgcggtgct ctacgcaccc gccgacagcg gcgcctccct ccggcccggc gccaaggtcg
     5941 acctcaccgt gcagtcggtg cctgcccaga gctacggggt gctgcgcggc accgtgaagt
     6001 cggtcggccg taccgcccgg acccagcagc agatctccgc cgacctcggc tccacccagc
     6061 tcggcgagga gttctcccgg aacgggccgc cgatcgccgt cgaggtccgc ctccacccca
     6121 cgaagagcac ccgcagcggc tacacctggt cgacctccga cggcccgccg cacccgctca
     6181 ccaccctcac cctcgtcgac ggcgccgtcc acctggacga acagcgcccg gccgattggc
     6241 tcctcccgtg accgcacccg ccgacgcccc cgccgccggg accggctcca ccgccccgcc
     6301 caccccgggc ggccgccggg gccgccgcgc cgtcccctcc tccggccgcc gccgcgccgc
     6361 gcccccgcgc cgcacgcccc gcaggcggcc cgtccgcacc cccaccgtcc tccagatgga
     6421 ggcggtggag tgcggcgccg cctgcctcgc catggtcctc gcccaccacg gacggcacgt
     6481 cccgctggag gagctgcgga tcgcctgcgg cgtctcccgc gacggctcgc gcgccagcaa
     6541 cgtcctgaaa gcggcccgca gttacggcct gaccgccaag ggcatgcaga tggagccggc
     6601 cgccctgcgc gaggtgcggg cccccgccgt gctgttctgg gagttcaacc actacgtcgt
     6661 ccacgagggg ttcctgcggc gccccgggcg gcgcggcgcg gtccgcctca acgaccccga
     6721 caaggggcgc cgggtggtga gcgccgagga gttcgacgcc tcgttcaccg gtgtcgccct
     6781 ggtcctggag ccgggctcct cgttccgcaa gggcggccgc aagcccgggc tgctgggcgc
     6841 gctcccggcc cggctgcgcg gcaccggcgc caccctgctg acggcgctcc tggccagcct
     6901 gctgctggtg gtggtgggcg cggcgctgcc cgcgctgagc cggacgtaca tcgacctgtt
     6961 cctgatcggg gagcggacct cggtgctcgg gccgctcttc gcggcgatgg gcgcgctggt
     7021 ggcgctgacc gcctcgctga ccgcgctcca gcaggccaat ctgctgcgcg ggcggatcat
     7081 ctcctcgacg ctgagcagtg cccggttcct gcgccatctg ctgcggctgc cggtgacctt
     7141 cttcgcccag cgctcccccg ccgacctggt gcagcggctc cagtcgaacg acacggtggc
     7201 cgagaccctc gcccgcgacc tggcggccgc cgccgtcgac ggggtggtgg tggtgctgta
     7261 cgcgctgctg ctgtggacgt acgacccgca actgaccctg gtcggggtgg ggatcgcgct
     7321 gctgaacatc gtggcgatgc gggtggtgat ccagctgcgg gccacccgga cgcagaagct
     7381 gcgggccgac aacgcgcggc tcaccaccac cgcgtacacc gggctccagc tcatcgagac
     7441 gatgaaggcg accggcggcg aggacgggtt cttccggcgg tgggcggggc agcacgcgac
     7501 gacgctggag gagcagcagc ggctcggggt gccgacggcg gtgctgtcgg tggtggcccc
     7561 ggcgctcgcc accttcaaca gtgcgctgat cctgtggatc ggcggactgc gggcggtcga
     7621 ggggcatctc tcggtcgggc tgctggtggc cttccaggcg ctgatggtgc ggttcacggc
     7681 gccgctgacc cggctcaacg gcgtcgccgg ccgcatccag gacttcgcgg cagacgtgac
     7741 ccggctgcgg gacgtcgagg cgttcccggc ggacgggctg tacgaacagg agggcggcgg
     7801 gagcggcgag gcggacaccc ggcggctggc gggccgggtg gagctggagg ggctgacctt
     7861 cggttacagc ccgctggacg caccgctgct gacggagttc tcgctgacgg tcgggccggg
     7921 gcggcaggtg gcgctggtcg gcgggtcggg cagcggcaag tcgacggtct cccggctgct
     7981 cgcgggcctg taccagccgt gggagggcac catccgcatc gacggccaac ccctcaccga
     8041 catcccgcgc tccgcgctgg ccgcctcggt ctccttcgtc gaccaggaca tcttcctctt
     8101 cgagggcacg gtgcgggaga acgtcaccct ctgggacccc tcactgccgg acgaggcggt
     8161 ccgcaccgcg ctgcgcgacg ccgggctgga ggaggtgatc gcccgccgcc cggacggcat
     8221 ccacgcccgg gtcgagcagg acgggcggaa cttctccggc ggccagcggc agcggctgga
     8281 gatcgcccgg gccctggtgc gccgccccag cgtgctggtg ctggacgagg tgaccagcgc
     8341 cctggacgcg gagaccgagc agcgggtgat ggcggccctc aggcggcgcg gctgcgcctg
     8401 cgtgatcatc gcgcaccgcc tctccaccgt ccgcgacagc gacgagatcg tggtgctgga
     8461 cggcggccgg gtcgtggaac gcggccgcca cgccgaactg gtcgcccggg gcggggcgta
     8521 cgcggccctg gtcgaggaga agtgagatga cgggcaccca gcgggcttcc catgccaccc
     8581 cggccgccga cccggtgacg gcggcgctcg gcggactcgg cgcaccggtg gagcgcgccg
     8641 aagggtcggc cctgccgctc ggcgggccct cggcgctctg gctggtcacc gagggcagcc
     8701 tcgacctctt cgcggtggcg gccgccgggg acggccactg gcactacctc ggccgcctgg
     8761 aggccggcac gctcctgctc ggcgccgtcg agggcccccg ccacaccctg gtgggccgac
     8821 tctcctcccg ctgcgccgta cgccgcctgc cgctgcgcga gctgtacgcg tcgcagggcc
     8881 ccgacgggta cgagggatac gaggggtacc ccggatacga gccggtcgcc gggccgctgg
     8941 agcacgcggt gggactcggc ctcggccggg ggctcgccgt gttgttcgcg gcaccgcagg
     9001 cggacggccc ggcgcccggg gcggggcccg ccgacgagga cgtgttgtgg gtggacgtcc
     9061 cgccgggcag tgtccggctg ggggcggagt tcgccgccga ggcggcggcc gacctgctgg
     9121 tcgacggggc gctgtggcag tccctggtgg accagcagta ccgcctgctg accgccgtcg
     9181 acggctgggt ggagcggctg gagcgggccc acgaggaccg ggcggcggcc ggtatccgcg
     9241 ccggtgaggc ggtacgggag cgggccgacc aggcgctgct cgactcgatc ggccgccgcg
     9301 accgctttcc gagggcgcgc ggcgaggcgg gcgacgacgc ggtgtacgcg gtcttccgcc
     9361 aggtggcgcg ggcgagcggc atcgcgctgc cggaggcggc ggcgcgttcg gtcgcgggcg
     9421 gcgtgcggct cgacccggtg gagcggatgg ccaccgccgc ccggctgcgg acccgcccgg
     9481 tccggctggc cggcgagtgg tggcgtaccg atgccgggcc gctggtgggg cggcgcgcca
     9541 agtccggcgc gccggtggcc ctgctctggc ggcgcggccg gtacgaggcg ctcaacccgg
     9601 cgaccggcgc ccgtatccgg atcggcgcca aggaggcggc cgagttcgag gaacgcgcca
     9661 cgatgctcta ccggccgctc cccgagggcc cgctgacgct gcgccggctg gtgctcttca
     9721 gcctgcgcgg ctcccggggc gacctgcgca acctcgtcct gagcggcctg gtcaccgtgg
     9781 cgctgggcgc gctggtgccg gtggcgaccg ggcaggtgct cggcgcgtac gtgcccagtg
     9841 cgcggaccgg gctgatcgcg cagatgtgtg tggcggtgat ggtggccggg gtggtctcgg
     9901 cggcgttcat gctgctccag tcgctgacgc tgctgcgtct ggaggggcgg atcgaggcgg
     9961 cgttgcagcc ggccgtctgg gaccggctgc tccggctgcc gacggccttc ttcgcggacc
    10021 gctccaccgg tgagctggcc agtgccgcga tggggatcag cgcgatccgc cgggtgctgt
    10081 cggggatcgg gccgaccgcc gtgcagtcgg tgacggtggc cggggtcaac ctggtgctgc
    10141 tgctggtgca cagcgtgccg atggcactgg cggcgatcgg ggtgctgctg gtggtctcgg
    10201 tggtcttcct ggtgct
//
