LOCUS       NZ_KZ195572            10945 bp    DNA     linear   CON 06-SEP-2024
DEFINITION  Streptomyces sp. CS057 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ195572
VERSION     NZ_KZ195572.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS057
  ORGANISM  Streptomyces sp. CS057
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 265.32x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188345.1-RS_2024_09_06
            Annotation Date                   :: 09/06/2024 02:40:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,152
            CDSs (total)                      :: 7,074
            Genes (coding)                    :: 6,991
            CDSs (with protein)               :: 6,991
            Genes (RNA)                       :: 78
            rRNAs                             :: 10, 6 (16S, 23S)
            complete rRNAs                    :: 10, 6 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 23 of 83
            Pseudo Genes (incomplete)         :: 71 of 83
            Pseudo Genes (internal stop)      :: 4 of 83
            Pseudo Genes (multiple problems)  :: 14 of 83
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:25:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8271853
            Orig. end                         :: 8282798
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195572.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
FEATURES             Location/Qualifiers
     region          1..10945
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="36"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10945
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10945
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5945](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5945
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(627..1487)
                     /locus_tag="B9W61_RS35380"
                     /old_locus_tag="B9W61_35350"
     CDS             complement(627..1487)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971205.1"
                     /locus_tag="B9W61_RS35380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_35350"
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="WP_087767620.1"
                     /transl_table=11
                     /translation="MSQHTVRPFLRRGSTLAGLAGGVVVAAGAGLLLSHAFASGSAAKA
                     APEVVPPSANVTFDYQIGGAYTPPAGVGAVSRDRGDEPADGLYNVCYVNAFQAQPDALG
                     WWEENHPDLLLRDEGGDLVEDEAWGEALLDTSTADRRTRLADIVGGWIDGCAKAGFQAV
                     EPDNLDSFERSDDLLTAEHNAAFAKLLADRAHDAGLAIGQKNTTDLLERRGRIGFDFAV
                     AEECGRYDECADYASAYDDRVFVVEYTDRDFTKACSSVGAKVSVVRRDLDVRPAGQPGY
                     VFRAC"
     gene            complement(1743..2813)
                     /locus_tag="B9W61_RS35385"
                     /old_locus_tag="B9W61_35355"
     CDS             complement(1743..2813)
                     /GO_function="GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042: O-methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459230.1"
                     /locus_tag="B9W61_RS35385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_35355"
                     /product="methyltransferase"
                     /protein_id="WP_087767621.1"
                     /transl_table=11
                     /translation="MTDRHDQDAGNGREEPVDWEARGAVARLVLGQMATQALGTAVRLG
                     VFDRIGPDELSAEALAGSLGTHPQATLRLLRALAGLRLLSETEPGAFRTTAAGDALRAD
                     TPGTMVAMARMFTDPVMLRGWDLLDESVRTGETTFDTVFGTDFFGHLKEHPELSASFNE
                     AMSQGTRLTAATVPHHYDFGRFHTLVDVGGGDGTLLASVLREHPELRGVLFDTAEGLAQ
                     APRRLAREGLTERVTLETGDFFASAPAGGDLYLLKSIIHDWDDERCAAILRHIRDVIPD
                     HGSLLIVEPVLPATVPPDRQDNVYLSDLNMLVNVGGRERTADDFAALCTAGGFALRSVT
                     PLPAPNIFQIVEASPV"
     misc_feature    2830..2848
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 25.4"
                     /tool="antismash"
     misc_feature    complement(2835..2853)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 25.73"
                     /tool="antismash"
     gene            complement(2920..3840)
                     /locus_tag="B9W61_RS35390"
                     /old_locus_tag="B9W61_35360"
     CDS             complement(2920..3840)
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956394.1"
                     /locus_tag="B9W61_RS35390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_35360"
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="WP_087767622.1"
                     /transl_table=11
                     /translation="MMSELPLDQVRTLLAVVDEGTFDSAAKALRLTPSAVSQRVKALEQ
                     RTGRVLLMRTKPVRPTESGEVVVRFARQLARLEHDAQTALGMSGPGEPTILPIAVNSDS
                     LATWFLPALRRVPEELGLCYELRREDQDHTAALLREGLAMAAVTSSPEAVTGCSVRRLG
                     RMRYLPVASPGFADRWLGRREGTALRELIADAPVIAFDRRDDLQDAFVRRLGSGPRASA
                     RRHLVPTSEGFANAVATGMGWGMVPEVQAEPLLSSGRLVLLTPDHTVDVPLYWQQWKLE
                     SPALAAVSEAVAAQAAEALDGDWPD"
     misc_feature    3836..3850
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.4"
                     /tool="antismash"
     misc_feature    3871..3885
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.23"
                     /tool="antismash"
     gene            3908..4519
                     /locus_tag="B9W61_RS35395"
                     /old_locus_tag="B9W61_35365"
     CDS             3908..4519
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459226.1"
                     /locus_tag="B9W61_RS35395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_35365"
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_087767623.1"
                     /transl_table=11
                     /translation="MTNALTTAAAGFGTGLSLIVAIGAQNALVLRQGVRRDGVLAVVGI
                     CALSDAVLISLGVAGVGALVVAWPSALTVVGLLGGVFLIGYGLLAARRVLRPSALHAGD
                     APTASRRGVVLTCLAMTWLNPHVYLDTVFLLGSVAADRGPLRWTFGLGAVLASVCWFVA
                     LGFGARLLSGVLARPSAWRVLDGLVAVMMIGLGAKLALTA"
     gene            5001..5945
                     /locus_tag="B9W61_RS35400"
                     /old_locus_tag="B9W61_35370"
     CDS             5001..5945
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971211.1"
                     /locus_tag="B9W61_RS35400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_35370"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_047174957.1"
                     /sec_met_domain="AfsA (E-value: 7.9e-26, bitscore: 88.6,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEAAVLIDPVPTMDAEAEVVHPVEIDMVHRTRPEDAFPQNWVRL
                     GHDRFSVGAVLPHDHPFFAPVGDDRHDPLLVAEAMRQAAMLAFHAGYGIPIGYQFLMSG
                     LDYVCHQEHLTVGGAPTEIDVEVFCSDLKWRGGLPAQGRVGWAVHRDGRLAATGMGETR
                     FSTPKAYRRMRGEAPAEGISIPVTAPVPPVLAGRARAEDVVLSETGRDGVWELRVDTRH
                     PTLFQRPNDHVPGMLLLEAARQAAWLAVGPAGIVPTEARTRFHRYSEFGSPCWIGAVVL
                     PETAEDTVTVQVTGHQDGEAVFSTVLSGPRADG"
     gene            5958..6827
                     /locus_tag="B9W61_RS35405"
                     /old_locus_tag="B9W61_35375"
     CDS             5958..6827
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387428.1"
                     /locus_tag="B9W61_RS35405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_35375"
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_087767624.1"
                     /transl_table=11
                     /translation="MILVTGATGAVGREVAGQLAAAGPVRILARRPERLTVRGEGVEVV
                     EGEYGDRAALDRALRGVASVFLVTNSPTEPDDERVAAAAAAAGVRHLVKLSMMAVEEPG
                     ADDFITRRQRENEQAVRESGVPWTFVRPRTFMSNTLSWAPGIRSTGVVRALYGDAPVAC
                     VDPRDVAAVAVAVLTGTGHEGRAYAVSGPEAITAREQTAQLSRVLERPLRFEELGVDAA
                     RTALLAKYPPPVAEAFLQSAERQRAGAKAAVVPTIQDLTGRPARPYRIWSAEHAEAFAP
                     EAGRFAGE"
     gene            7017..7763
                     /locus_tag="B9W61_RS35410"
                     /old_locus_tag="B9W61_35380"
     CDS             7017..7763
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /GO_function="GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA]"
                     /GO_process="GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459221.1"
                     /locus_tag="B9W61_RS35410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_35380"
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="WP_047174955.1"
                     /transl_table=11
                     /translation="MADENLLHPRLAALYDPLDPDRGDLQPYVRLAEEFGARRVLDIGC
                     GTGVFALLLADRGIDVVGVDPDRASLDVARAKPGGDRVRWIHGDAAALPPLRVDLVTLT
                     ANAAQEIVDPGDWEATLHRAHEALRPGGHLVFETRDPARRAWETWNRESSHRVTEIPDV
                     GPVETWVEVTGIVGPLVSFRWTYVFRRDGQVLTSDSTLRFRERQEAEADLEAQGFVVRE
                     VRGAPDRPGRELVLIARRPGPAPRRR"
     misc_feature    complement(7026..7041)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.01"
                     /tool="antismash"
     misc_feature    7032..7034
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(7723..8649)
                     /locus_tag="B9W61_RS35415"
                     /old_locus_tag="B9W61_35385"
     CDS             complement(7723..8649)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459219.1"
                     /locus_tag="B9W61_RS35415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_35385"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_047174954.1"
                     /transl_table=11
                     /translation="MDDQPEPAPEPLDRRAELSEFLRTRRAKLRPGDVGLPEFGRHRRV
                     PGLRREELAQLAGVSVAYYTRLEQGNGRNVSAEVLDAIARALRLTDAESAHLTHLAKPA
                     RHKKKRRPARVQRVRKGLLYLLDSMDGIPAYVTGARSDVLAWNAMAAAVFGDWGALPPA
                     ERNWARLVFLSPAYRDLFVNWDSKASDMVSYLRLYAGCHPDDPELSALVGELSVKSDEF
                     RRLWATHNVKEKGHGVKLLRHPLVGELTLSYETLKLPDDEEQHLVTYHAEPGSDSAQSL
                     RLLASWGADAGLTDVIGDAGRGPAGER"
     misc_feature    complement(8734..8749)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.13"
                     /tool="antismash"
     gene            8812..9852
                     /locus_tag="B9W61_RS35420"
                     /old_locus_tag="B9W61_35390"
     CDS             8812..9852
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (19-268). E-value: 2.2e-12.
                     Score: 38.6. Matches aSDomain:
                     nrpspksdomains_B9W61_RS35420_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761872.1"
                     /locus_tag="B9W61_RS35420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_35390"
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_087767626.1"
                     /sec_met_domain="ADH_N (E-value: 3.5e-28, bitscore: 96.4,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.2e-22, bitscore:
                     79.0, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVAAYAAPRAKAPLERTTIERRPVGEFDVLIDIKFAGICHSDI
                     HQARDGWGEGIFPMVPGHEIAGIVAETGSGVTRFKVGDRVGVGCMVDSCGKCDACLMGR
                     EQHCAEGNTQTYNALDRAGRPTYGGYSTHIVVAETFALSIPEGIALDEAAPLLCAGITT
                     YSPLKRWGAGPGKKVAVVGLGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHY
                     YATSDPRTFEELAGAFDVILSTVSAPLDFGAYLGLLRTDGTLVNVGAPEEPVSLNLFSL
                     ILGNKSIAGSAIGGIKETQEMLDFCAVHGLGAEIEVIEAGQVNEAYERVLASDVRYRFV
                     IDTATI"
     aSDomain        8869..9615
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9W61_RS35420_PKS_ER.1"
                     /evalue="2.20E-12"
                     /label="B9W61_RS35420_PKS_ER.1"
                     /locus_tag="B9W61_RS35420"
                     /protein_end="268"
                     /protein_start="19"
                     /score="38.6"
                     /tool="antismash"
                     /translation="TIERRPVGEFDVLIDIKFAGICHSDIHQARDGWGEGIFPMVPGHE
                     IAGIVAETGSGVTRFKVGDRVGVGCMVDSCGKCDACLMGREQHCAEGNTQTYNALDRAG
                     RPTYGGYSTHIVVAETFALSIPEGIALDEAAPLLCAGITTYSPLKRWGAGPGKKVAVVG
                     LGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHYYATSDPRTFEELAGAFDVI
                     LSTVSAPLDFGAYLGLLRTDGTLVNVG"
     misc_feature    10015..10028
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.17"
                     /tool="antismash"
ORIGIN
        1 ccggtctgcc cacggccgtc tcctggaccc cgtacttcgc cgtcgaggac gtcgacgccg
       61 caacggcccg ggtccgcgaa cgcggcggca ccatcgccgt aggcccgatc cggctcggcc
      121 ccgggcgcgg agccgtggcc gccgaccgcg aaggggccgg attcggcgtc tgggaaggcc
      181 gcaccccgcc ctggtcgttc ggccgcgacc ggccgcccgc ctccctcgaa ctgcggacga
      241 gccacgcctt cgactccgcg atcttctact ccgaggtgtt cgactgggcc tccgaggagc
      301 ctggcggctg cgaggtcggc tacgcatacc agcaggacac ggtcgtcgta cggcagggcg
      361 gccgcaccgt ggccaccctg cgcgggggcg gggacgcgtc gtcgcccgac gcccggctgc
      421 gcccgcgctg gcacgtccgc ttccgggtcg cggacctgga agcggccacc ggtgcggcgc
      481 gcgcggcggg tggcacggtc ggcccgccgg tggactcgcc cgccgacggg tccgaagcgg
      541 tgatcaggga ccccggcggt ggcatcttca ccgtctgcgc ccacccgccg gacaccccct
      601 gagcccggcc gccccgggaa ggcccttcag caggcgcgga acacgtaccc cggctgtccg
      661 gccggccgga cgtcgaggtc gcggcgcacc accgagacct tcgcccccac ggacgaacac
      721 gccttcgtga aatcgcgatc ggtgtactcg acgacgaaca ccctgtcgtc gtacgcggag
      781 gcgtagtcgg cgcactcgtc gtaccggccg cactcctcgg cgacggcgaa gtcgaacccg
      841 atcctcccgc gccgctccag cagatcggtg gtgttcttct ggccgatcgc cagcccggcg
      901 tcgtgcgccc ggtcggccag gagcttcgcg aaggccgcgt tgtgctccgc ggtcagcagg
      961 tcgtcggagc gctcgaagga gtccaggttg tccggctcca ccgcctggaa gccggccttc
     1021 gcgcagccgt cgatccagcc cccgacgatg tccgccagcc gggtccgccg gtccgcggtc
     1081 gaggtgtcga gcagcgcctc gccccaggcc tcgtcctcga cgaggtctcc gccctcgtcg
     1141 cgcagcagca gatcgggatg gttctcctcc caccagccca gcgcgtcggg ctgggcctgg
     1201 aaggcgttga cgtaacagac gttgtacagg ccatcggccg gctcgtcgcc acggtcgcgg
     1261 gagaccgccc cgaccccggc gggcggggtg tacgcgccgc cgatctggta gtcgaaggtc
     1321 acgttcgccg agggcggcac gacctcgggc gcggccttcg ccgcggagcc cgaggcgaag
     1381 gcgtgggaca ggagcagtcc cgctcccgcc gcgaccacga cgccaccggc caggccggcg
     1441 agggtgcttc cgcggcggag gaacggacgg acggtgtgct gggacatacg tgactcccgt
     1501 gcatgggctt cggaacctga ccccacatca tggacggccg atcatgagga cgaactccgc
     1561 gccgccgccg ggtgggcccg gacggtgagc gacccccggt gggccgccgc gatccggcgt
     1621 accagcggga ggccggtgcc cgttcgtgcg gcggcacacg caggggagga tgcctccgag
     1681 tcttcttccg ggggaggccc cgtcattcta aggtggggcg ggcacccgcc ccccgggggc
     1741 cgtcagaccg gggaggcctc gacgatctgg aagatgttgg gcgcgggcag tggtgtcacg
     1801 ctccgcaggg cgaagccgcc cgccgtgcac agggccgcga agtcgtccgc ggtccgctcc
     1861 cggccgccga cgttgacgag catgttgagg tcgctgaggt agacgttgtc ctgccggtcc
     1921 ggcggcacgg tggcgggcag caccggttcg acgatcagca gggagccgtg gtcggggatg
     1981 acgtcgcgga tgtgccgcag gatggcggcg caccgctcgt cgtcccagtc gtggatgatg
     2041 ctcttgagca gatagaggtc gccgccggcc ggggccgagg cgaagaagtc gccggtctcc
     2101 agggtgacgc gctcggtcag accttcccgg gccagccgtc gaggagcctg cgcgagtccc
     2161 tcggccgtgt cgaacaggac gccccgcagc tccgggtgct cgcgaaggac cgaggccagc
     2221 agcgtgccgt cgccgccgcc gacgtccacg agtgtgtgga agcgcccgaa gtcgtagtgg
     2281 tgcgggacgg tcgcggcggt cagccgtgtg ccctggctca tcgcctcgtt gaacgaggcg
     2341 gacagctcgg ggtgctcctt gaggtggccg aagaagtcgg tcccgaacac cgtgtcgaac
     2401 gtggtctctc ccgtacggac gctctcgtcg agcaggtccc agccccgcag catgaccggg
     2461 tcggtgaaca tccgggccat ggcgaccatc gtgccgggtg tgtcggcgcg cagggcgtcg
     2521 ccggccgcgg tggtccggaa ggcgcccggt tccgtctcgg acagcagccg gagtccggcc
     2581 agggcgcgca gcagacgcag ggtcgcctgc gggtgggtgc cgagggaacc ggcgagcgcc
     2641 tcggcgctca gctcgtccgg cccgatccgg tcgaagacgc ccaaccgcac cgccgtgccc
     2701 agcgcctggg tggccatctg gccgagcacg agccgggcca ccgccccccg cgcctcccaa
     2761 tccaccggct cttcccggcc gttgcccgca tcctggtcgt gacggtccgt catcgcctgc
     2821 ccctttccgt tcagttaggc aaacctaacc tcggaggggt gggggagcaa tcacccgtag
     2881 gcgtggcgag ccgggttccg gtggcgggcg gtgaggtgct cagtcgggcc agtcgccgtc
     2941 gagcgcttcg gcggcctggg ccgccaccgc ctcggagacg gccgccaggg ccggtgactc
     3001 cagcttccac tgctgccagt acagcggcac gtccacggtg tggtccgggg tgagcagcac
     3061 cagccggccg ctgctcagca acggctccgc ctggacctcg ggcaccatgc cccagcccat
     3121 gcccgtcgcc acggcgttcg cgaacccctc cgacgtggga accagatggc gccgggcgct
     3181 cgcccggggt ccggagccga gccggcgcac aaaggcgtcc tgaaggtcgt cgcgccggtc
     3241 gaaggcgatc accggggcgt cggcgatcag ctcgcgcaac gcggtgccct cccgccgtcc
     3301 cagccaccgg tcggcgaaac cgggactggc caccggcaaa taccgcatcc ggcccagccg
     3361 gcgcaccgag cagcccgtca ccgcctccgg cgaagaggtc accgcggcca tcgccagtcc
     3421 ctcccgcagc agggcggccg tatggtcctg gtcctcccgc cgcagctcgt agcagagccc
     3481 cagctcctcc ggcacgcgcc gcagggcggg caggaaccag gtcgccaggg agtcggagtt
     3541 caccgcgatc ggcaggatgg tcggctcgcc ggggccggac atgccgagcg ccgtctgcgc
     3601 gtcgtgctcc agcctggcca gctggcgggc gaagcgcacc accacctcgc cggactcggt
     3661 gggccgcacc ggcttcgtac gcatcagcag cacccggccc gtgcgctgct ccagtgcctt
     3721 gacccgctgg ctgaccgccg acggcgtcag ccgcagagcc ttcgccgccg agtcgaacgt
     3781 gccttcgtcg accacggcca gcagggtccg cacctggtcg agagggagct cggacatcat
     3841 gactgttaat gatacgtaag aatctttagc tggacaccat gttctcccgc tccgtagcgt
     3901 cggccgcatg acgaacgccc tgaccaccgc ggccgccgga ttcggcaccg gcctctcgct
     3961 catcgtcgcc atcggagccc agaacgccct ggtgctgcgc cagggcgtcc gccgtgacgg
     4021 cgtgctcgcg gtcgtcggca tctgcgccct gtccgacgcg gtgctcatct cgctgggggt
     4081 cgccggggtc ggcgcgctcg tcgtcgcctg gccctcggcg ctgacggtgg tggggctgct
     4141 cggcggcgtg ttcctgatcg gctacggcct gctcgccgcg cgccgggtcc tgcgcccgtc
     4201 ggccctgcac gcgggggacg ccccgaccgc ctcacgccgg ggcgtggtgc tgacctgcct
     4261 ggcgatgacc tggctcaacc cgcacgtgta cctcgacacc gtgttcctgc tcgggtccgt
     4321 cgccgccgac cggggaccgt tgcgctggac cttcggcctc ggcgccgttc tcgccagcgt
     4381 gtgctggttc gtcgcgctcg ggttcggcgc ccggttgctg agcggagtcc tcgcgcggcc
     4441 ttccgcgtgg cgggtgctgg acggcctggt cgccgtgatg atgatcggcc tcggcgcgaa
     4501 gctggcgctg accgcctgac cgcctgaccg cctgaccgcc tgaccgcctg cgcccggggc
     4561 ctccccgggc gctccgggcg tccggggtcg agggtgctcg cccggcgtac catgccatga
     4621 tcgccatctt ccgacaggat cgtgccatgg gaagcgtgca gcgggccgtc gttctcgccc
     4681 tggacggatc gcatccgttc gagctcggta tcccgagccg ggtacccgtg gccgcacgtg
     4741 aaacggcagc ggatatcgcc tttcccgcag tcggcgcttt tgggaattcc ggtgattctc
     4801 gcggattggc ggacgcattt atcggatgcc cgcgccgctg tcatccggat tcccgggcgg
     4861 ggcccgcgaa tcggccgggg tggtgtgggg ggtctcaccc tggccgaagg aaaaaaccta
     4921 ttagtctgta cctgtaccaa atcttgccag tatgtgcatc gcaggaacgg ctcgccacta
     4981 catgacccct ggggggagtt atgcccgaag cagcagtctt gatcgatccg gtgccgacca
     5041 tggacgcgga ggccgaggtg gtgcatcccg tggagatcga catggtgcac cggaccaggc
     5101 cggaggacgc ctttccgcag aactgggtgc gcctgggtca cgaccggttc tcggtgggtg
     5161 cggttcttcc gcacgaccat ccgttcttcg ccccggtggg tgacgaccgg cacgacccgc
     5221 tgctggtcgc ggaggcgatg cggcaggcgg ccatgctcgc cttccatgcc ggttacggca
     5281 tcccgatcgg ctaccagttc ctgatgtcgg gactggacta cgtctgccat caggagcacc
     5341 tgaccgtggg gggcgccccc accgagatcg acgtggaggt gttctgctcc gacctgaagt
     5401 ggcggggcgg gctcccggcg caggggcgtg tcggctgggc ggtgcaccgg gacggtcggc
     5461 tcgccgcgac ggggatgggg gagacccggt tcagcacccc caaggcgtac cggcggatgc
     5521 gcggcgaggc cccggccgag gggatatcca tacccgtgac cgcgccggtt ccgcccgtcc
     5581 tggcgggccg tgcccgcgcc gaggacgtgg tgctctcgga gaccgggcgg gacggagtct
     5641 gggagctgcg cgtggacacc cggcatccca ccctgttcca gcgccccaac gaccacgtcc
     5701 cgggcatgtt gctgctggag gcggcccgcc aggcggcgtg gctggcggtg gggcccgccg
     5761 gcatcgtgcc gacggaggcg cgcacccggt tccaccggta ctccgagttc ggcagcccgt
     5821 gctggatagg ggcggtggtc ctgccggaga cggccgagga tacggtgacg gtccaggtga
     5881 caggccatca ggacggtgaa gcggtgttct ccaccgtcct gtccggcccc cgggccgacg
     5941 gctgagaggt ttctcgagtg attctcgtga cgggtgcgac cggagcggtg ggccgtgagg
     6001 tcgccggaca gctggcggcg gccggccccg tgcggattct cgcgcgccgt cccgagcggc
     6061 tcacggtgcg aggagaaggg gtcgaggtcg tcgaggggga gtacggggac cgtgccgctc
     6121 tcgaccgggc cctgcggggc gtcgcctcgg tgttcctggt gacgaacagc cccacggaac
     6181 ccgacgacga gcgggtcgcc gcggcggcag ccgcggcagg cgtacgccac ctggtcaagc
     6241 tgtccatgat ggcggtggag gagccgggcg ccgacgactt catcacccgg cgccagcggg
     6301 agaacgaaca ggcggtcagg gagtcggggg tcccgtggac gttcgtccgc cccaggacct
     6361 tcatgtccaa cacgctgtcc tgggcgcccg gcatcaggtc gacgggagtg gtgcgcgccc
     6421 tgtacggcga cgccccggtc gcgtgcgtcg accctcgtga tgtcgccgcg gtggccgtcg
     6481 ccgttctcac cggtacgggg cacgagggca gggcgtacgc ggtgtcgggc ccggaggcga
     6541 tcaccgcacg ggagcagacg gctcagctct cccgggtgct ggagcggccg ttgcgcttcg
     6601 aggaactggg cgtggacgcc gcgcgcaccg ccctgctggc gaagtatccg ccgcccgtcg
     6661 ccgaggcctt cctgcagagc gcggagcggc agcgggcggg cgcgaaggcg gccgtggtgc
     6721 ccacgatcca ggacctgacg ggccgtccgg cccggccgta ccgcatctgg tcggccgagc
     6781 acgcggaggc gttcgcgccg gaagccggtc gtttcgcggg cgagtaggac gcatcggcac
     6841 cctgcggccg cggatacgcg cgacatcgcg cgtattccct tttccgcccc gcttttgccg
     6901 ctctcggagc cggcggaccc gaaaaggccc gggagcctca gccgcatttc tccgttcccg
     6961 aaagggaaac cggtgagcac gttgcgccgc cggcaggacc ctgtaccttc cgccccatgg
     7021 ctgacgagaa tttattgcat ccccggctgg ctgccctcta cgacccgctc gatcccgacc
     7081 ggggcgacct gcaaccgtac gtccgcctcg ccgaggagtt cggcgcacgg cgggtgctgg
     7141 acatcggctg cggtacgggc gtgttcgccc tgctgctggc ggaccgcggg atcgacgtcg
     7201 tcggggtcga ccccgaccgg gcctcgctcg acgtggcgcg cgccaagccg ggcggggacc
     7261 gggtccgttg gatccacggt gacgccgcgg cccttccgcc gttgcgggtc gacctggtca
     7321 ccctgacggc caacgccgcg caggagatcg tcgacccggg cgactgggag gcgacactgc
     7381 accgcgccca cgaagcgctg cggcccggcg ggcacctggt attcgagacc cgggatccgg
     7441 cccggcgggc ctgggagaca tggaatcggg agagttccca ccgcgtcacc gagatccccg
     7501 acgtcggccc cgtcgagacc tgggtcgagg tgaccgggat cgtcgggccg ctcgtgtcgt
     7561 tccgctggac ctatgtgttc cggcgcgacg ggcaggtcct cacctccgac tccaccctcc
     7621 gcttccgtga acgtcaggag gccgaggcgg acctggaggc acaggggttc gtcgtacgcg
     7681 aggtgcgcgg cgcacccgac cggcccggcc gggaactcgt cctcatcgct cgccggccgg
     7741 gccccgcccc gcgtcgccga tgacgtccgt caatcccgcg tcggcacccc agctcgccag
     7801 caggcgcaag gactgggccg aatccgagcc cggctccgcg tggtaggtca ccagatgctg
     7861 ctcctcgtcg tccgggagct tcaacgtctc gtacgacagg gtcagctccc cgacgagcgg
     7921 atgccgcagc agcttgacgc cgtggccctt ctccttgacg ttgtgggtgg cccacagccg
     7981 ccggaactca tcgctcttga cggagagttc gcccaccagc gccgacagct ccggatcgtc
     8041 cgggtggcag cccgcgtaca gccgcagata gctgaccatg tccgaggcct tggagtccca
     8101 gttcacgaac aggtcccggt aggcggggga gaggaacacc agccgcgccc agttgcgctc
     8161 ggccggcggc aacgcgcccc agtcgccgaa caccgccgcc gccatcgcat tccaggcgag
     8221 cacgtcggag cgcgccccgg tcacgtacgc ggggatgccg tccatgctgt cgagcagata
     8281 cagcaacccc ttccgcaccc gctgcaccct ggccgggcgg cgcttcttct tgtgccgcgc
     8341 gggcttggcc aggtgcgtca gatgcgcgct ctcggcgtcc gtgagccgca gggcccgcgc
     8401 gatcgcgtcg aggacctcgg ccgacacgtt gcgcccgttg ccctgctcca ggcgcgtgta
     8461 gtaggcaacg gacaccccgg ccagctgtgc cagctcctcc cggcgcagac cgggcacccg
     8521 gcggtgccgg ccgaactccg gaagccccac gtcgcccggc cggagcttgg ccctccgggt
     8581 gcgcaggaac tcgctgagct cggcgcgccg gtccagcggc tcgggggcgg gctccggctg
     8641 atcgtccatg gcttccatta ttccggtgat ccctggtcgt acgcacctga gcctgtccct
     8701 gtcagtagta ggaccgctgg acgtaggcaa agcggtgacc tgggtaaaca tccgcagcgg
     8761 cgtgatgctg gatggcgttc ctaccgcatc acccagagga gaacctccgg catgaccacc
     8821 gttgccgcct atgccgctcc ccgtgccaag gctccgctgg agcgcaccac gatcgaacgc
     8881 cgcccagtgg gcgagttcga cgtcctgatc gacatcaagt tcgccgggat ctgccactcc
     8941 gacatccacc aggcccgcga cggctggggc gagggcatct tcccgatggt gcccggccac
     9001 gagatcgccg ggatcgtcgc cgagaccggc tccggcgtca cccggttcaa ggtcggcgac
     9061 cgcgtcggcg tcggctgcat ggtcgactcc tgcggaaagt gcgacgcgtg cctgatgggc
     9121 cgcgagcagc actgtgccga ggggaacacc cagacgtaca acgctctcga ccgcgccggc
     9181 cggcccacct acggcggcta ctcgacgcac atcgtcgtgg ccgagacgtt cgccctctcc
     9241 attcccgagg gcatcgccct ggacgaggcc gcgccgctcc tgtgcgcggg catcaccacg
     9301 tactccccgc tcaagcgctg gggcgcgggc cccggcaaga aggtcgccgt cgtcggcctc
     9361 ggcggtctcg gccacatggc cgtcaagatc gcgcacgcgc tcggcgccga ggtcacggtc
     9421 ctctcccagt cgctgcgcaa gaaggacgac gggctcaagc tgggagccga ccactactac
     9481 gcgaccagcg acccccggac gttcgaggag ctggccggcg ccttcgacgt catcctctcc
     9541 acggtctcgg ccccgctgga cttcggcgcc tacctcggtc tgctgcggac cgacggcacg
     9601 ctggtcaacg tgggcgcccc cgaggagccg gtctcgctga acctcttctc cctgatcctc
     9661 ggcaacaagt cgatcgcggg ttcggcgatc ggcgggatca aggagaccca ggagatgctc
     9721 gacttctgcg ccgtgcacgg gctcggcgcc gagatcgagg tgatcgaagc gggccaggtc
     9781 aacgaggcgt acgagcgggt tctcgccagc gacgtccgct accgcttcgt gatcgacacc
     9841 gcgacgatct gaggcgtcgg ccgccgggcc gccccaccac gcgcatgccc gccccgagcg
     9901 tcccaggtgc tcggggcgga cgcgtacccc tgcggctgcc ccctccgagg aaaggggagg
     9961 tggcccccgg ccgtgctggg gtgccagccc cagtgatccc gaacggcccc tctcctaacg
    10021 tctgttggtg tgagttacgc agaggacacc gagaagatca cccgaagccc ggcggccgcc
    10081 cgcaccaccg cggccacgac gacgaccgcc aggaccgccg ccgcgggcct ggccctggcc
    10141 cttgccgcgg gcctcaccgc gtcaccggcc ctggccaccg cacggcccgg accggaaccc
    10201 cggaccgacg caccggcgac ggccggcgga gcgctcgacc gctactacca ccagcccctg
    10261 gactggggca cctgcatgcg ctccccggag gacgggacgg gcagggacct ggacaaggcg
    10321 ggcgtgcaat gtgcggacgt cacggtcccg ctggactaca gcgcccccgg gggccggacg
    10381 accacggtcg cggtatcccg tctgaaggcc accgacaccc gcaaccgcat cggcgcgatc
    10441 ctcctcaaca acggcggccc cggcggcccg gccgtggact ccccgccggt cgtccgcacg
    10501 gcgatgaagg aggtcggccc ccgctacgac atcgtcggtt tcgacccgcg cttcgtcggc
    10561 aggagcaccc cgctggactg cgactggccg gtcggcttca cctggttctc cgccggtgcg
    10621 agcagggcgg gcttcgaccg ccaggtcgcc ctgagcaaga gcctggcagc caagtgccgc
    10681 gccacgaacg cctcggtgct cccgcacatc accacccgca acacggcacg cgacatggac
    10741 gtgatccggg gcgcgctggg cgagcggaag atctcctacc tgggctattc gtacggtacc
    10801 tacctgggca cggtctacac gcagatgttc cccggccgct acgaccggat ggtcctggac
    10861 ggagcggtgg gcccggacga ctacagccca cgtctgctga agcgcacggt gaccgagaac
    10921 gagcaggcgc tctccgcctg ggcca
//
