LOCUS       NZ_KZ195572            10399 bp    DNA     linear   CON 06-SEP-2024
DEFINITION  Streptomyces sp. CS057 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ195572
VERSION     NZ_KZ195572.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS057
  ORGANISM  Streptomyces sp. CS057
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 265.32x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188345.1-RS_2024_09_06
            Annotation Date                   :: 09/06/2024 02:40:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,152
            CDSs (total)                      :: 7,074
            Genes (coding)                    :: 6,991
            CDSs (with protein)               :: 6,991
            Genes (RNA)                       :: 78
            rRNAs                             :: 10, 6 (16S, 23S)
            complete rRNAs                    :: 10, 6 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 23 of 83
            Pseudo Genes (incomplete)         :: 71 of 83
            Pseudo Genes (internal stop)      :: 4 of 83
            Pseudo Genes (multiple problems)  :: 14 of 83
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:25:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6825312
            Orig. end                         :: 6835711
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195572.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="29"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    221..234
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.28"
                     /tool="antismash"
     gene            270..1328
                     /locus_tag="B9W61_RS29095"
                     /old_locus_tag="B9W61_29055"
     CDS             270..1328
                     /NRPS_PKS="Domain: Aminotran_5 (19-347). E-value: 9.2e-24.
                     Score: 75.9. Matches aSDomain:
                     nrpspksdomains_B9W61_RS29095_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012381431.1"
                     /locus_tag="B9W61_RS29095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_29055"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_047176017.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7.8e-16, bitscore:
                     56.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTILAPIRAAAVEFAPEVTYLNTSSWGLLPRRTIAAVKALADENA
                     AGRRVGAGSFDAVEAARVGFARIAGVRPDRVATGSSVTVHVGLIAASLPPGAEVLCPEG
                     EFSSVVSPFALRGDLRVRYAPLDGLAEAVRPSTALVAFSAVQSADGRVADLAAVRAAAA
                     AHGARTLLDGTQAAGWFPLEAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAQDTLPPLFA
                     GWVSAGAPWTSNYGPLERLAPTARGFDEPPAFLSYHGAEHSLGLLAEVGADALYAHATG
                     LAARLRAGLAGLGHGSVPGESAIVSVPGLEDRQPDLVKAGIAVSAPAGNLRISCHLYNT
                     EADVDRVLEVLA"
     aSDomain        327..1310
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9W61_RS29095_Aminotran_5.1"
                     /evalue="9.20E-24"
                     /label="B9W61_RS29095_Aminotran_5.1"
                     /locus_tag="B9W61_RS29095"
                     /protein_end="347"
                     /protein_start="19"
                     /score="75.9"
                     /tool="antismash"
                     /translation="YLNTSSWGLLPRRTIAAVKALADENAAGRRVGAGSFDAVEAARVG
                     FARIAGVRPDRVATGSSVTVHVGLIAASLPPGAEVLCPEGEFSSVVSPFALRGDLRVRY
                     APLDGLAEAVRPSTALVAFSAVQSADGRVADLAAVRAAAAAHGARTLLDGTQAAGWFPL
                     EAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAQDTLPPLFAGWVSAGAPWTSNYGPLERL
                     APTARGFDEPPAFLSYHGAEHSLGLLAEVGADALYAHATGLAARLRAGLAGLGHGSVPG
                     ESAIVSVPGLEDRQPDLVKAGIAVSAPAGNLRISCHLYNTEADVDRV"
     gene            complement(1345..1851)
                     /locus_tag="B9W61_RS29100"
                     /old_locus_tag="B9W61_29060"
     CDS             complement(1345..1851)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007450490.1"
                     /locus_tag="B9W61_RS29100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_29060"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_087766909.1"
                     /transl_table=11
                     /translation="MTTGRATPAEDACTNALPMAALSGPVGHAVSRVARLHRTAAGKAL
                     REVDLYPGQELLMMHLWDRGAVRQTELIRALDLDPSTVTKMLQRLEQTGHVRRRPDPAD
                     RRAVLVEATDESCALHTAVRDAWSGLEEHTLAGLDPAERTELVRLLAKVEENLCRETAD
                     CPDRC"
     gene            2004..3074
                     /locus_tag="B9W61_RS29105"
                     /old_locus_tag="B9W61_29065"
     CDS             2004..3074
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969934.1"
                     /locus_tag="B9W61_RS29105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_29065"
                     /product="alkene reductase"
                     /protein_id="WP_047176015.1"
                     /transl_table=11
                     /translation="MTTAFDPIDLAGTQLANRIALAPMTRSRAGEGGTATELTATYYAQ
                     RASAGLVITEGIQPSVVGQGYPTTPGLHSAEQVASWRKVTDAVHAEGGRIFAQIMHAGR
                     IGHPVLLPDGLVPVSASPVKAEGQVYTHVGPKDFVEPHELTDAEIHATVADFVTASRNA
                     VEAGFDGVELHGANGYLIQQFLAPNTNLRTDAWGGSDEARIRFAVEVVKAVAAEIGPER
                     TALRISPGNPYNDIDEPAPEATYTALVQAIEPLGIAYLHILEAAPIRELTLALRKAFSG
                     TVVINVHSDGATGPDDHTVIDDGIADLISYGSLFLANPDLPARLKAGGPFNTPDPSTFF
                     GGDAKGYTDYPALDAV"
     gene            complement(3050..4087)
                     /locus_tag="B9W61_RS29110"
                     /old_locus_tag="B9W61_29070"
     CDS             complement(3050..4087)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070337.1"
                     /locus_tag="B9W61_RS29110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_29070"
                     /product="VOC family protein"
                     /protein_id="WP_087766910.1"
                     /transl_table=11
                     /translation="MTSHVRHITIDCADAHALGGFWAQVLGAPLSDEDRPGDPEALLET
                     PGAAILFVRVDEKKRTKNRVHLDIQPQDRSRDEEVERLLALGATLVGDHRRPNGRGWAT
                     LADPEGNEFCVECSAAERAALTGTRLPVTADDVTSAVRLAVDVLAGAPADRWDAPAGSL
                     DWTCWETVEHLSDDLFAYAVQLGPRTPPLDRDVPYRWAPERQGGPYNAVFADRDAGPAG
                     LLATLEASGALLASMARTTPPEVRSYHGYGISDPEGFAAMGVVETLVHTYDLAEGLGLD
                     WSPSPALCDRVLARLFPDAPAGGDRWAVLLWATGRAELPDRPRRTSWRWDGRPREEGQT
                     ASSAG"
     assembly_gap    4132..4156
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4157..4994)
                     /gene="thpD"
                     /locus_tag="B9W61_RS29115"
                     /old_locus_tag="B9W61_29075"
     CDS             complement(<4157..4994)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="B9W61_RS29115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_29075"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_087768036.1"
                     /sec_met_domain="PhyH (E-value: 2.6e-38, bitscore: 131.1,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEVRADLYPSRGAAEMTTPRQDPVIWSAPGAPGPVAAKDLQGY
                     EHDGFLTVDQLISPDEVAVYRAELDRLISDPAVRADERSIVEKQSQNVRSVFEVHRISE
                     VFAGLVRDERVVGRARQILGSDVYVHQSRINVKPGFGATGFYWHSDFETWHAEDGLPNM
                     RTVSVSIALTENFDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPSDEALT
                     KMADRHGIRLFTGKAGSATWFDCNAMHGSGDNITPYARSNVFIVFNSVENAAQEPFA"
     gene            complement(5001..5399)
                     /locus_tag="B9W61_RS29120"
                     /old_locus_tag="B9W61_29080"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045195.1"
                     /locus_tag="B9W61_RS29120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_29080"
                     /product="ectoine synthase"
                     /protein_id="WP_003969937.1"
                     /sec_met_domain="ectoine_synt (E-value: 9e-54, bitscore:
                     179.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFSDIENTDRHVKSASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGETHWITPGTMYLLDGHERHTMRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEA"
     gene            complement(5486..6748)
                     /gene="ectB"
                     /locus_tag="B9W61_RS29125"
                     /old_locus_tag="B9W61_29085"
     CDS             complement(5486..6748)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 1e-71.
                     Score: 233.7. Matches aSDomain:
                     nrpspksdomains_B9W61_RS29125_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="B9W61_RS29125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_29085"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_087766911.1"
                     /sec_met_domain="Aminotran_3 (E-value: 6e-76, bitscore:
                     254.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALLDYIERDGITHGLDMATTAKRAFLETFQNVILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQELCHRQDMLLIVDDIQMGCGRTGGFFSFEEAGIVPDIVTLSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAVLDAYWADGQMEKQTLARGEQVEQTLL
                     AICAEEPTAQFRGRGLVWGMEFEDPARASAVCARAFQLGLLLETSGPQSEVVKLLPPLT
                     ITPEELDEGLRTLARSVRETA"
     aSDomain        complement(5660..6664)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9W61_RS29125_Aminotran_3.1"
                     /evalue="1.00E-71"
                     /label="B9W61_RS29125_Aminotran_3.1"
                     /locus_tag="B9W61_RS29125"
                     /protein_end="363"
                     /protein_start="28"
                     /score="233.7"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALLDYIE
                     RDGITHGLDMATTAKRAFLETFQNVILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQELCHRQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGIVPDIVTLSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAVLDAYWADGQMEKQTLARGEQVEQTLLAICAEEPTAQFRGRGLVWGMEFE"
     gene            complement(6906..7436)
                     /gene="ectA"
                     /locus_tag="B9W61_RS29130"
                     /old_locus_tag="B9W61_29090"
     CDS             complement(6906..7436)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123622.1"
                     /locus_tag="B9W61_RS29130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_29090"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_075347816.1"
                     /transl_table=11
                     /translation="MTAAPADFARARSEFLSIDAPRVEDGAAIWRIARDSQVLDLNSSY
                     SYLLWCRDFAATSAVARGEDGEPIAFVTGYVRPDRPQTLVVWQVAVDRAHRGKGLAAAL
                     LDELTARVAADQGLSSVETTITPDNTASDRLFTSYAQRHDVALEKEVLFDGELFPEETH
                     LPEVLYRIGPFAT"
     misc_feature    7447..7461
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.51"
                     /tool="antismash"
     misc_feature    complement(7461..7473)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(7592..7607)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7602..7617
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7602..7619)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     misc_feature    7677..7693
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.18"
                     /tool="antismash"
     misc_feature    complement(7690..7705)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.4"
                     /tool="antismash"
     misc_feature    complement(7708..7726)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.85"
                     /tool="antismash"
     gene            complement(7746..8852)
                     /locus_tag="B9W61_RS29135"
                     /old_locus_tag="B9W61_29095"
     CDS             complement(7746..8852)
                     /NRPS_PKS="Domain: Aminotran_5 (16-284). E-value: 2.9e-28.
                     Score: 90.7. Matches aSDomain:
                     nrpspksdomains_B9W61_RS29135_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957694.1"
                     /locus_tag="B9W61_RS29135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_29095"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_075347817.1"
                     /sec_met_domain="Aminotran_5 (E-value: 9.6e-22, bitscore:
                     76.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLTADHFAAIERRVARLLATEQDVVITQGEALLPLE
                     GCIRGGARPGSTALNVVTGPYGQTFGNWLRDCGAEVVDLTVPFHTAVTADQVAEALAEH
                     PGIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSARAWERIAANPAAPRRSYLSLLDWKERWIDGGRTALPHAP
                     AQLEMLGLEACVARIEDEGLETVMARHASAAAATRAGAVALGGGLEPYVHAARDAAPVA
                     TTLRAPAGTDAAALVAAALAADPALPLVAGGGALSKEMIRVNHYGADATRGAVLSSLAA
                     LGAVLTDAGRRVDIEAARRAVSETWSSV"
     aSDomain        complement(8001..8804)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9W61_RS29135_Aminotran_5.1"
                     /evalue="2.90E-28"
                     /label="B9W61_RS29135_Aminotran_5.1"
                     /locus_tag="B9W61_RS29135"
                     /protein_end="284"
                     /protein_start="16"
                     /score="90.7"
                     /tool="antismash"
                     /translation="AAIERRVARLLATEQDVVITQGEALLPLEGCIRGGARPGSTALNV
                     VTGPYGQTFGNWLRDCGAEVVDLTVPFHTAVTADQVAEALAEHPGIDFVSLVHAEAATG
                     NTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVSAV
                     SVSARAWERIAANPAAPRRSYLSLLDWKERWIDGGRTALPHAPAQLEMLGLEACVARIE
                     DEGLETVMARHASAAAATRAGAVALGGGLEPYVHAARDAAPVATTL"
     gene            8984..10084
                     /locus_tag="B9W61_RS29140"
                     /old_locus_tag="B9W61_29100"
     CDS             8984..10084
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123619.1"
                     /locus_tag="B9W61_RS29140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_29100"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_087766912.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.3e-16, bitscore:
                     59.4, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDQEEQRVLRVKGRVLVGPDEVRDELWAVGGRITYERPPGADRA
                     VTVRGWALPGLVDAHCHVGLDRHGPVDAATAEQQALTDREAGTLLIRDAGSPSDTRWID
                     DREDLPKVIRAGRHIARTRRYIRNYAHEIEPGDLVAYVAREARRGDGWVKLVGDWIDRD
                     AGDLTACWPRGEVEAAIAEAHRLGARVTAHCFAEESLTDLVEAGIDCIEHATGLTEDTI
                     PLFAERGVAIVPTLVNIATFPDLAAGGEAKFPRWSAHMRQLYDRRYDTVRAAYDAGIPV
                     YVGTDAGGSLAHGLVAGEVAELVKAGIPPLEALSATAWGARSWLGRPALEEGAPADLVV
                     YEEDPRADVRVLAAPRHIVLNGRVIG"
ORIGIN
        1 ccgtgccgtt cttcgtcctc gaccgccggt acgggatctc cggcggccag ccgtcggagg
       61 tcttcgtcca ggcactggag caggcctgga aggaccgtcc cgccaccgcg ctgaccaccg
      121 tcggcgggga cgcggcgggc tgcgacgcgg acggggcctg cgaggtcccg gggagctgag
      181 cccgcccgga cccgcttata agcattcctt tggtgccccc ttgactctcc gtgattgact
      241 ttgggtcaag ggggcttcag ggtggatgga tgacgatcct tgcgcccatc cgggcggcag
      301 ccgtggaatt cgcgcccgag gtcacctatc tcaacacctc cagctggggc ctgctgcccc
      361 gccggacgat cgccgccgtc aaggccctgg ccgacgagaa cgccgcgggg cgccgggtgg
      421 gggcgggcag cttcgacgcg gtggaggccg cccgggtcgg cttcgcccgg atcgccgggg
      481 tccgcccgga ccgggtcgcc accgggagtt ccgtcaccgt ccacgtcggt ctgatcgccg
      541 cttcactgcc gccgggtgcc gaagtcctct gtcccgaggg cgagttctcc tccgtggtca
      601 gcccgttcgc gctccgcggc gatctgcgcg tgcggtacgc gccgctcgac ggcctcgccg
      661 aggccgtgcg cccctcgacc gccctcgtcg ccttctccgc cgtgcagtcc gccgacggcc
      721 gggtggccga tctcgccgcc gtccgcgcgg ccgccgcggc gcacggggcc cggaccctgc
      781 tggacggcac ccaggcggcg ggctggttcc cgctggaggc gggggcgtac gactacaccg
      841 tcaccggcgg cttcaagttc ctgctctgtc cacgcggtac gtcgttcctg acggtcaccg
      901 aggaggcgca ggacaccctg ccgcccctct tcgccggctg ggtctccgcc ggtgcaccct
      961 ggacgagcaa ctacggcccg ctggagcgcc tcgcccccac cgcccgcggc ttcgacgagc
     1021 cgcccgcctt cctctcgtac cacggggccg aacactccct gggcctgctc gccgaggtgg
     1081 gtgccgacgc cctgtacgcg cacgccaccg ggctcgcggc ccggctgcgg gcggggctgg
     1141 cggggctcgg tcacggatcc gttcccgggg agtcggcgat cgtctccgtg ccgggtctgg
     1201 aggaccgtca gcccgacctg gtgaaggccg ggatcgccgt ctcggccccg gcggggaatc
     1261 tgcggatctc ctgccacctc tacaacaccg aggccgatgt ggaccgggtg ctggaggtcc
     1321 tggcctgatc cgtacgggtg cggttcagca ccggtccggg cagtccgcgg tctcccggca
     1381 gaggttctcc tcgaccttcg ccagcagccg gaccagctcc gtgcgttcgg ccgggtcgag
     1441 gcccgcgagc gtgtgctcct ccaggccgct ccaggcgtcc cggaccgccg tgtgcagcgc
     1501 gcagctctcg tccgtcgcct cgaccagcac ggcccgccgg tcggccgggt cggggcggcg
     1561 gcgcacatgc ccggtctgct ccagccgctg gagcatcttc gtgaccgtgg acgggtccag
     1621 gtccagggcc ctgatcagct ccgtctgacg gaccgcgccc cggtcccaca gatgcatcat
     1681 cagcaactcc tgccccgggt agaggtccac ctccctgagc gccttgcccg ccgccgtccg
     1741 gtgcagccgg gccacccggg acacggcatg gccgacgggc ccgctgagcg ccgccatggg
     1801 cagcgcgttc gtacaggcgt cttccgcggg ggtggcgcgg ccggtggtca tggcgggctc
     1861 ctggggttcc tcggtggtgc ggacggcggt tctcgatgat gcccgacggt ttcccgcaac
     1921 tttaccttgg tcggccaatg aatgggttac agttgccgcc ggtgccgata cttggccgac
     1981 cacatacttt cttgggagcc ctgatgacca ctgctttcga cccgatcgac ctggccggca
     2041 cccagctggc caaccggatc gccctggccc cgatgacccg cagccgggcc ggtgagggcg
     2101 gtaccgccac cgagctcacc gccacgtact acgcccagcg cgcctcggcc ggcctcgtga
     2161 tcaccgaggg catccagccg tccgtcgtgg gccagggcta ccccaccacg ccggggctgc
     2221 acagcgccga acaggtcgcc tcctggcgca aggtcaccga cgccgtgcac gccgagggcg
     2281 gccggatctt cgcgcagatc atgcacgcgg gccgcatcgg ccacccggtc ctgctcccgg
     2341 acggcctggt ccccgtcagc gcctccccgg tcaaggccga ggggcaggtc tacacccacg
     2401 tcgggccgaa ggacttcgtc gagccgcacg agctgacgga cgccgagatc cacgccaccg
     2461 tcgccgactt cgtcaccgcc tcccgcaacg ccgtggaagc cggcttcgac ggggtcgagc
     2521 tgcacggcgc caacggctat ctcatccagc agttcctggc ccccaacacc aacctgcgca
     2581 ccgacgcgtg gggcggctcc gacgaggccc gcatccgctt cgccgtcgag gtcgtcaagg
     2641 ccgtcgccgc cgagatcggc ccggagcgca ccgcactgcg catctcgccc ggcaacccct
     2701 acaacgacat cgacgagccc gctccggaag ccacgtacac cgccctggtg caggcgatcg
     2761 aaccgctcgg catcgcctac ctgcacatcc tggaggccgc cccgatccgg gaactgaccc
     2821 tcgccctgcg caaggcgttc tccggcaccg tcgtcatcaa cgtgcactcc gacggcgcca
     2881 ccggacccga cgaccacacc gtgatcgacg acggcatcgc cgacctgatc tcctacgggt
     2941 ccctcttcct cgccaacccg gacctgcccg cccggctcaa ggcgggcggc ccgttcaaca
     3001 cccccgaccc gtcgaccttc ttcggcggcg acgccaaggg ctacaccgac tacccggcgc
     3061 tcgacgccgt ctgaccctcc tcgcgaggcc gcccgtccca ccgccacgag gtacggcgcg
     3121 gacggtccgg cagttcggcg cgaccggtgg cccagagcag tacggcccac cggtcgccgc
     3181 ccgccggggc gtccgggaag agccgggcca gcacccggtc gcagagcgcc ggggacggcg
     3241 accagtccag ccccaggccc tccgccaggt catacgtgtg caccagggtc tccaccacgc
     3301 ccatcgcggc gaaaccctcc gggtcggaga tcccgtaccc gtggtacgag cgcacctcgg
     3361 gcggcgtcgt ccgcgccatc gaggccagca gcgccccgct cgcctccagc gtggccagca
     3421 gcccggcggg accggcgtca cggtcggcga agaccgcgtt gtagggcccg ccctgccgct
     3481 cgggcgccca ccggtagggc acgtcccggt ccaacggggg cgtacggggc cccaactgca
     3541 cggcgtacgc gaacagatcg tcgctcaggt gctccaccgt ctcccagcag gtccagtcga
     3601 ggcttcccgc gggcgcgtcc cagcggtccg ccggggctcc ggccagcaca tccacggcga
     3661 gccgtaccgc cgaggtcacg tcgtcggcgg tcaccggcag ccgggtcccg gtgagcgccg
     3721 cccgctcggc ggcggagcac tccacacaga actcgttgcc ctcgggatcg gcgagcgtcg
     3781 cccagccgcg cccgttcggc ctgcggtggt cgcccaccag ggtcgcgccg agcgcgagca
     3841 gccgctccac ctcctcgtcc cgggagcggt cctgcggctg gatgtccaga tggacgcggt
     3901 tcttggtccg cttcttctcg tccacccgga cgaagaggat cgccgcgccg ggcgtctcca
     3961 gcagggcctc cggatcgccg ggccggtcct cgtcggagag cggcgcgccg agcacctggg
     4021 cccagaagcc gccgagcgcg tgggcgtcgg cgcagtcgat cgtgatgtga cgtacatggg
     4081 aagtcatggc ggacaccctc tccgctgacc cggccacggt ccagcgagtt annnnnnnnn
     4141 nnnnnnnnnn nnnnnnccgc gaagggctcc tgcgccgcgt tctccacgct gttgaagacg
     4201 atgaagacgt tgctgcgcgc gtacggggtg atgttgtcgc ccgagccgtg catggcgttg
     4261 cagtcgaacc aggtcgccga accggccttg ccggtgaaga gcctgatgcc gtggcggtcg
     4321 gccatcttcg tcagcgcctc gtcggacggg gtgccggcgt cctgcatctg gagcgacttc
     4381 ttgtagttgt ccttcggcgt ctcgcccgca cagccgagga acgtcttgtg cgaaccgggc
     4441 atgatcatca gcccgccgtt ggtgtcgaag ttctcggtca gcgcgatcga cacggacacc
     4501 gtccgcatgt tcggcagacc gtcctccgcg tgccaggtct cgaagtccga gtgccagtag
     4561 aagcccgtgg ccccgaagcc cggcttcacg ttgatccggg actggtggac gtacacgtcc
     4621 gagccgagga tctggcgggc ccggcccacc acccgctcgt cgcggaccag accggcgaag
     4681 acctcgctga tccggtggac ctcgaagacg gaccgtacgt tctgcgactg cttctcgacg
     4741 atcgagcgct cgtcggcgcg gaccgccggg tcggagatca gccggtccag ctccgcccgg
     4801 tagacggcga cctcgtccgg ggagatgagc tggtcgacgg tgaggaagcc gtcgtgctcg
     4861 taaccctgga ggtccttggc ggcgaccgga cccggtgcgc ccggcgcgga ccagatgacc
     4921 gggtcctggc ggggagtggt catctcggcg gcgccgcgcg aggggtacag atcggcgcgt
     4981 acttcggtgg tcatggtggt tcaggcctcc tcggtcagca gtgggtagac accgttctcg
     5041 tcatggtcct cccgtccggt gacgggaggg ttgaagacac acacgcagcg gaagtccgtc
     5101 ttgggccgca tggtgtgccg ctcatgcccg tccaggaggt acatggtccc gggcgtgatc
     5161 cagtgcgtct cgccggtctc gtcgttggtg agctcggcct cgccctcggt gcacagcacc
     5221 gcctcgatgt ggttcgcgta ccacatggag gtctccgtgc ccgcgtacag cacggtctcg
     5281 tggagcgaga agcccacctt ctccttggcg agcacgatgc gcttgctctc ccaggtgccg
     5341 gacgcggact tcacatgccg gtcggtgttc tcgatgtcac tgaaagatcg gacgatcacg
     5401 gtgagcggtt gcctttctct ctgtacagag tcccgggcct cgcggcccgg gacctctctt
     5461 ctccgcctgc cccggcagtg ccgggtcagg ccgtttcgcg gacggagcgg gccagcgtgc
     5521 gcaggccctc gtccagctcc tcgggggtga tggtcagcgg cggcagcagc ttgacgacct
     5581 cgctctgcgg gcccgaggtc tccagcagga gccccaactg gaaggcgcgg gcgcagaccg
     5641 ccgaggcgcg cgccgggtcc tcgaactcca tgccccacac caggccgcgg ccgcggaact
     5701 gcgcggtcgg ctcctcggcg cagatggcga gcagggtctg ttcgacctgt tcgccgcggg
     5761 cgagggtctg cttctccatc tggccgtcgg cccagtaggc gtcgagtacg gctgcggcgg
     5821 tgacgaaggc cgggttgttg ccgcggaagg tgccgttgtg ctcgccgggc tcccagatgt
     5881 cgagctcggg cttgaagagg cagagggaca tgggcaggcc gtagccgctg atggacttgg
     5941 agagggtgac gatgtccggg acgatgccgg cctcctcgaa ggagaagaag ccgccggtgc
     6001 ggccgcagcc catctggatg tcgtcgacga tcagcagcat gtcctggcgg tggcacagct
     6061 cctggagcgc gcgcagccac tcggcgcggg cgacgttgat gccgccctcg ccctggacgg
     6121 tctccacgat cacggcggcg ggcttgttga gcccggagcc ctggtcctcc aggagccgct
     6181 cgaaccagag gaagtcgggg acggtgccgt cgaagtagtt gtcgaacggc atcggggtgc
     6241 cgtgcaccag cgggatgccg gccccggccc gcttgaacgc gttcccggtc accgcgagcg
     6301 agcccagcga catgccgtgg aaggcgttgg tgaacgagac gaccgactcg cggcccttga
     6361 ccttacgggc cagcttcagc gccgcctcga cggcgttcgt gccggtcggg ccggggaaca
     6421 tcaccttgta cggcagatcg cgcggccgca ggatcacgtt ctggaacgtc tccaggaacg
     6481 cccgcttggc ggtggtcgcc atgtccaggc cgtgggtgat gccgtcgcgc tcgatgtagt
     6541 ccagcagcgc tcgtttcagc accgggttgt tgtggccgta gttgagcgag ccggccccgg
     6601 cgaagaagtc caggtaggag tggccgtcct cgtcggtcag ccgggcaccc tgcgcgcggt
     6661 cgaacacggc gggccagccg cggcagtagc tccgtacctc cgactccagg gtctcgaaga
     6721 cgctcagggc gggcggggtg atggtcacag cgggtctcct gcgtgagggg ggtgtgacgg
     6781 gcagtggtcg attgctcccc gccgcacggg tgcggccggg accgcgccga gtggtcggtc
     6841 ggcgcgaaaa gtcggtgggt cccggtgtac tggtcgggta cgccggggtg cggtggccgg
     6901 gcggctcagg tggcgaacgg gcctatccgg tagagcacct cgggcaggtg cgtctcttcg
     6961 gggaacagct cgccgtcgaa gagcacttcc ttctccagcg cgacgtcgtg gcgctgggcg
     7021 taggaggtga acagccggtc cgaggcggtg ttgtccggcg tgatcgtcgt ctccacggac
     7081 gacagaccct ggtcggcggc gacccgggcg gtcagctcgt ccagcagagc ggcggccagc
     7141 cccttgccgc ggtgggcccg gtcgacggcc acctgccaga cgacgagggt ctgggggcgg
     7201 tcgggccgga cgtagcccgt cacgaaggcg atgggctcgc cgtcctcgcc acgggcgacg
     7261 gcggaggtcg cggcgaagtc acggcaccac agcaggtagc tgtacgagga gttcaggtcc
     7321 aggacctggg agtcgcgggc tatgcgccag atcgcggctc cgtcctccac tcgtggcgca
     7381 tcgatactga ggaattcgct tcgggcacgg gcaaaatctg ctggtgcggc ggtcatgcga
     7441 attgaattta cccagcaaat ttcgaaattg catcgacgta gagggttggg tggaggctcc
     7501 ttctgtgcta tcaggcgtgc gcgagacccc tcgcgattcg gacacatttt ccggcagttt
     7561 gtcctgaatt tatcgggcat aacgccgatc ttgtggagtc ggtcacaggg ttgtaactct
     7621 cgcgagatgt gtccgaatca tgaagttcgc ggccagaaaa aactggcccg tttaggtgac
     7681 gggatagcgg gcaaaagaat gtgggaagct gcttttcgat aaagcagctt tcctcatgag
     7741 gaatatcaga cgctcgacca ggtttccgaa acagccctac gggcagcctc gatatcgacc
     7801 cgccggcccg catcggtcag caccgccccc agagccgcga gcgaggaaag caccgcgccc
     7861 cgcgtcgcgt ccgccccgta atggttgacc cggatcatct ccttggacag cgccccgccg
     7921 cccgcgacga gcggcagcgc ggggtccgcc gccagcgccg ccgcgaccag cgcggccgcg
     7981 tccgtccccg ccggggcgcg cagcgtcgtc gccaccgggg ccgcgtcccg cgccgcgtgg
     8041 acgtacggct ccagtccgcc gcccagggcc accgcaccgg cccgggtggc cgcggcggcc
     8101 gaggcgtggc gggccatcac cgtctccagg ccctcgtcct cgatccgcgc cacgcaggcc
     8161 tccaggccca gcatctccaa ctgtgccggg gcgtgcggca gcgccgtacg accgccgtcg
     8221 atccagcgct ccttccagtc caggagggag aggtaggagc ggcgcggggc ggccgggttg
     8281 gcggcgatcc gctcccaggc ccgcgcgctc accgacaccg ccgacacccc ggcgggcccg
     8341 cccatcgcct tctgcgcgcc gatcacgcac aggtcgacgc cccaggcgtc cggcagcagc
     8401 ggctcggcgc ccaccgaggc gaccgcgtcc agcatgaaca gcgcgccgtg cgcccggacc
     8461 acctcgccga tctccgcgac cgggttggtg ttgccggtcg ccgcctccgc gtgcaccagc
     8521 gagacgaagt cgatccccgg gtgctcggcg agcgcctcgg cgacctggtc ggcggtgacc
     8581 gcggtgtgga agggcaccgt gaggtcgacg acctcggcgc cgcagtcccg cagccagttg
     8641 ccgaaggtct gcccgtacgg tccggtgacc acgttcagcg cggtcgagcc gggccgtgct
     8701 ccgccgcgga tgcagccctc cagcggcagc agtgcctcgc cctgggtgat gacgacgtcc
     8761 tgctccgtgg cgaggagccg ggccacgcgc cgctcgatcg ccgcgaagtg gtcggcggtg
     8821 agcgggggga ggtcgaggaa ggggtgcgtc acggtggggc tctcttcggg ttcgttcgcg
     8881 ggtccttggg cgggcgccgg tgaaacgttc ccgccttccg ggtgggcggc gggaacgtcc
     8941 acgggcccca gcagcctacc gacggcctcg tacagtgccg cgtatgagtg atcaggagga
     9001 gcagcgggtg ctgcgggtga agggccgggt gctcgtcggc ccggacgagg tgcgggacga
     9061 actctgggcc gtgggcgggc ggatcaccta cgagcggccg cccggcgccg accgcgcggt
     9121 gacggtcagg gggtgggcgc tgcccggact ggtcgacgcg cactgccatg tgggtctgga
     9181 ccggcacggc ccggtcgacg cggcgaccgc cgagcagcag gcgctcaccg accgggaggc
     9241 cgggacgctg ttgatccggg acgcgggatc gccctccgac acccggtgga tcgacgaccg
     9301 cgaggacctt ccgaaggtca tcagggccgg ccgccacatc gccaggaccc gccgctacat
     9361 ccgcaactac gcccatgaga tcgaacccgg cgacctcgtc gcgtacgtgg cccgggaggc
     9421 ccggcgcggg gacggctggg tcaagctggt gggggactgg atcgaccgcg acgccgggga
     9481 cctgaccgcc tgctggccgc gcggcgaggt cgaggcggcc atcgccgagg cgcaccggct
     9541 cggcgcccgc gtcaccgcgc actgcttcgc cgaggagtcg ctcaccgacc tggtggaggc
     9601 gggcatcgac tgtatcgagc acgccaccgg cctcaccgag gacaccatcc cgctcttcgc
     9661 cgaacggggg gtggcgatcg tcccgacgct ggtcaacatc gccacgttcc ccgatctcgc
     9721 ggcgggcggc gaggccaagt tcccccgctg gtcggcccat atgcggcagc tgtacgaccg
     9781 ccgctacgac accgtgcgcg ccgcgtacga cgccgggatt cccgtctacg tcggcaccga
     9841 cgcgggcggc tccctggcgc acgggctggt cgcgggcgag gtcgccgaac tcgtcaaggc
     9901 gggcatcccg cccctggagg ccctctccgc caccgcctgg ggggccagga gctggctggg
     9961 gcggcccgcg ctggaggagg gcgctccggc ggacctggtc gtgtacgaag aggacccgcg
    10021 cgcggacgtc cgggtgctgg cggcgccccg ccacatcgtg ctgaacgggc gggtgatcgg
    10081 ctgagggcct gtgcggtgcc gtgttgacga gaagtgacgg atacggtccc ggactcgttg
    10141 aagcacctct gcgcatgctc cggcgcgagc gcgctccgta cggctcgcgc cgggtgaacc
    10201 gcgggacatc agcgaacgcg tgtgccgaaa gattcgaata tgcgcgcgga aaccccactt
    10261 tggggtgaac tcacgccagg tatccgccag ttcaccctca gtgcgtaaag attcacggag
    10321 tcgaggccgt cggcacaggc gatgtccccc attggactgt gccggcggct ccatgtctct
    10381 tgtgggggtt ccaccatct
//
