LOCUS       NZ_KZ195572            10423 bp    DNA     linear   CON 06-SEP-2024
DEFINITION  Streptomyces sp. CS057 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ195572
VERSION     NZ_KZ195572.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS057
  ORGANISM  Streptomyces sp. CS057
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 265.32x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188345.1-RS_2024_09_06
            Annotation Date                   :: 09/06/2024 02:40:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,152
            CDSs (total)                      :: 7,074
            Genes (coding)                    :: 6,991
            CDSs (with protein)               :: 6,991
            Genes (RNA)                       :: 78
            rRNAs                             :: 10, 6 (16S, 23S)
            complete rRNAs                    :: 10, 6 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 23 of 83
            Pseudo Genes (incomplete)         :: 71 of 83
            Pseudo Genes (internal stop)      :: 4 of 83
            Pseudo Genes (multiple problems)  :: 14 of 83
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:25:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2957642
            Orig. end                         :: 2968065
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195572.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
FEATURES             Location/Qualifiers
     region          1..10423
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="21"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10423
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10423
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5423](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5423
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            508..720
                     /locus_tag="B9W61_RS36860"
                     /old_locus_tag="B9W61_12160"
     CDS             508..720
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472730.1"
                     /locus_tag="B9W61_RS36860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_12160"
                     /product="hypothetical protein"
                     /protein_id="WP_075347608.1"
                     /transl_table=11
                     /translation="MAREELQRLTGDNGNGGPCGQGDCPNVYRTSSGSIVVQGDVSDAF
                     TPPPGEGLVEIPEAVLREAIHALGW"
     misc_feature    523..525
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(532..545)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.57"
                     /tool="antismash"
     gene            704..1216
                     /locus_tag="B9W61_RS12185"
                     /old_locus_tag="B9W61_12165"
     CDS             704..1216
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956222.1"
                     /locus_tag="B9W61_RS12185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_12165"
                     /product="DUF6879 family protein"
                     /protein_id="WP_087765096.1"
                     /transl_table=11
                     /translation="MLLAGDEWAAKFRDFRSEAWRLETLPQYLVPQEADEFQAFKEGAR
                     YPGPYEDDWMRMVRTRTVGRVHIVRRPLSAYLEFEFDRYYRHQAPAGEDIRILDVTDRP
                     NPLPDTLSDFWMFDRSTVVLMNYEEDGTQINRQLAEGDVSPFLAYQRIAVAESVPFEEY
                     VRGGHRA"
     gene            1200..2036
                     /locus_tag="B9W61_RS12190"
                     /old_locus_tag="B9W61_12170"
     CDS             1200..2036
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956221.1"
                     /locus_tag="B9W61_RS12190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_12170"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_087765097.1"
                     /transl_table=11
                     /translation="MAIEPDALDRTGLELAAMLRQLRKQAGLSGARLAARCNMSQSKVS
                     RIEGHRVRPSLVDVERILKGLGASPAVAAEAMAMARIAQTEWQDHKALHRKGLDKKQRE
                     LAGLEAATTDFRFFLLSMVTGLLSTPEYIRASILDAPPTQQARAVAMKLERQAVLLDRS
                     KRFTFILTEQAVRYPLVAPDAMAMQIDRISSLARQTNISILLLPLSARYSNTPLSTFTV
                     YDDRLATIETDLGSAVFRDPRDIRSLLDRFAFYEDQAVTDEAVQELLSQWAWSFRN"
     gene            2178..2408
                     /locus_tag="B9W61_RS12195"
                     /old_locus_tag="B9W61_12175"
     CDS             2178..2408
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956220.1"
                     /locus_tag="B9W61_RS12195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_12175"
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_087765098.1"
                     /transl_table=11
                     /translation="MIDGKKLYALDISSARFAKACGGNTHPDGESCVTLARIGDDAWAL
                     GDSKRPDATPLRFTTAELEAAGITPVAFGLA"
     gene            complement(3112..3531)
                     /locus_tag="B9W61_RS12205"
                     /old_locus_tag="B9W61_12185"
     CDS             complement(3112..3531)
                     /EC_number="3.1.2.-"
                     /GO_function="GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1144: thioesterase superfamily protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966557.1"
                     /locus_tag="B9W61_RS12205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_12185"
                     /product="PaaI family thioesterase"
                     /protein_id="WP_087765100.1"
                     /transl_table=11
                     /translation="MEAQSSELAFGQKVLEAQPFSVLLGARLTAFGDGAAVLELDVRDE
                     LRQQNGFVHGGVLSYAADNALTFAAGAAVGPSVLTAGFTIDYLRPARGEVLRARAQVVR
                     PGRTRVVCRCDLLMVDAAGVETLCAVAQGTIAVAG"
     gene            3637..4647
                     /locus_tag="B9W61_RS12210"
                     /old_locus_tag="B9W61_12190"
     CDS             3637..4647
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006127309.1"
                     /locus_tag="B9W61_RS12210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_12190"
                     /product="DUF6301 family protein"
                     /protein_id="WP_087765101.1"
                     /transl_table=11
                     /translation="MENMIPRGNWLDDDIFRKLVREVTPLRFGSWSLREFEHATSELGW
                     ELREPREAAGRVRRRFAPRKGPWGGYGTVISDASEPDRVRKLNVRVVDLPAEDMATAAG
                     FVRAAWWVMEEELGPPTMWGGDSGPWMLWRHPGACLLVHSHDEGEVSLELLPPDADTDA
                     AGNGYSRGRWRAAEQADLPTVRPASDTTWEEVEKRLSETLRSLDHDTPFFPGRFILHLG
                     SADDPQRFVQCWSQDRGLVVEATGYLHRPEAVDATRLADNGWDSSRSVWQRRFPDAMDQ
                     PAHAATGARMLVEELRHLGVDPADLSYDGTMTGRGRGFHLELPDLGIPRVHHPAE"
     gene            5001..5423
                     /locus_tag="B9W61_RS12215"
                     /old_locus_tag="B9W61_12195"
     CDS             5001..5423
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966560.1"
                     /locus_tag="B9W61_RS12215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_12195"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_087765102.1"
                     /sec_met_domain="melC (E-value: 7.1e-40, bitscore: 135.1,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGPTRRQALGAAIGVAAGGALVGVTSASADTVPAGTGSEGSAAG
                     LAGTPGSFDEVFEGRRIQGTPAEAHASAHAHGHAGHGAGYRVLIDGRELPVMQHGKSGW
                     SSTINHYERFATPLEAARTAVISLKGAVVVPFDPTA"
     gene            5440..6264
                     /locus_tag="B9W61_RS12220"
                     /old_locus_tag="B9W61_12200"
     CDS             5440..6264
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012379204.1"
                     /locus_tag="B9W61_RS12220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_12200"
                     /product="tyrosinase family protein"
                     /protein_id="WP_087765103.1"
                     /transl_table=11
                     /translation="MTVRKNQANLTAPEKKAFVDAVLEVKRLGVYDEFVLAHRTRFSID
                     MNTGVYVGHFGPSFLPWHRKFLIDFEAELQKIDPSVSIPYWDWTADNTVGSSLWAPDFL
                     GGTGRPLDDQVMDGPFAHSAGNWTITVSVDSRPFLARNLAHRVPTLPPRSDVDAALALP
                     TYDSPPFRDGSAGFRSALEGSAGYMSMHNRVHTWVWGHMETSVSPNDPVFWLHHAFVDK
                     LWADWHALHPTSAEYLPAGGTTDVIDLNDTMPPWPNTTPQDMLDHTLFYTYA"
     gene            complement(6415..6564)
                     /locus_tag="B9W61_RS36145"
     CDS             complement(6415..6564)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B9W61_RS36145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="SMI1/KNR4 family protein"
                     /protein_id="WP_239112145.1"
                     /transl_table=11
                     /translation="MPHPAVVRLRELLPPPSSGGDAVDWEQIERTTGLVFPADYREFVS
                     WRGR"
     gene            7005..7303
                     /locus_tag="B9W61_RS12225"
                     /old_locus_tag="B9W61_12205"
                     /pseudo=""
     CDS             7005..7303
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477291.1"
                     /locus_tag="B9W61_RS12225"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_12205"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLIVTREGDRRCKLHPSQRAMVALVVYLREHEPDFVLLDGTLAEC
                     DRVGEGRADWPQHERPSNAGPQD"
     misc_feature    7608..7622
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.19"
                     /tool="antismash"
     misc_feature    7655..7669
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.37"
                     /tool="antismash"
     gene            8075..8407
                     /locus_tag="B9W61_RS12230"
                     /old_locus_tag="B9W61_12210"
     CDS             8075..8407
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B9W61_RS12230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B9W61_12210"
                     /product="hypothetical protein"
                     /protein_id="WP_143666262.1"
                     /transl_table=11
                     /translation="MKKFATAAAIAMGGLALVISTQGSAQASAPAAQASAQTSAQQTDD
                     VRPQALGGLVKAAGKAAGKAYVHAKAACPSVGKAAGEVAGNFLGGSVAPADKIGNAEAI
                     DTVFDK"
     gene            8431..9042
                     /locus_tag="B9W61_RS12235"
                     /old_locus_tag="B9W61_12215"
     CDS             8431..9042
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040205.4"
                     /locus_tag="B9W61_RS12235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_12215"
                     /product="DUF5819 family protein"
                     /protein_id="WP_143666263.1"
                     /transl_table=11
                     /translation="MALIIGAALLGIYFLFASFSQMPLTPMKVKYYSQVNDVMQPYMAQ
                     NWMLFAPDPLSDERGILSRARCADGTATDFYDVTGPYVQKVQGDRFFPSRINRLVSGTV
                     AQLDSSDPVLQRLRDTEKEAKKKQLPLLPYEKTSREQAERFLGRYSLTQMPGVCAGRPA
                     AVQVRVYVHELPPWSQRKDESAKGSTKYQDLSWREVDTLK"
     gene            9039..10082
                     /locus_tag="B9W61_RS12240"
                     /old_locus_tag="B9W61_12220"
     CDS             9039..10082
                     /GO_function="GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523516.1"
                     /locus_tag="B9W61_RS12240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_12220"
                     /product="HTTM domain-containing protein"
                     /protein_id="WP_087765106.1"
                     /transl_table=11
                     /translation="MRKILEKLDGWTLSPVSVVGVSGARVLLGFVGLMYYVSQYGDRAF
                     LFGPDSVLPWQEFADRVAENDSFSLYAISSSSAWFEIVFHLGIIIALLVMLGVGGRVGL
                     AVHWIFLWSIYERQTVLLDGGDNLAYLVIPMLLLTRCYDRFSFPSGIGAKLASRLPDRV
                     RVTATPLHNLGVIAIALQMCLVYVVSGLYKVQGQMWQDGTALFYVLRVPEFSFPALSHL
                     VYENDFLVFSGTYVTVLFLVYFPLGVLVPALRPWAAVMSIGFHLGIGLLMGLTGFALTM
                     VACDLVFLSRGLDEAVTYATTRLQKARSRRLEKPAAQGSHDGNTAGSRQVKEDHTSPEP
                     VGSGKAG"
ORIGIN
        1 tgcggcacgc catgggcgac cggcccatca ccgtcaacgg cggcttccgc agcgtgtcct
       61 gcaacagcgc ggtcggcgga gccgccaaca gccgccacat gtacgggcac gcggcggacc
      121 tcggcgcggg ctcccagggc ttctgcggcc tcgcccaggc ggcccgcaac cacggcttca
      181 ccgagatcct cggcccgggc taccccggcc acaacgacca cacccacgtc gccgggggca
      241 gcggccgctt ctggtccgcg cccagctgcg gcatctgagg cgcggcggtc ccatgaagtg
      301 cgggggcttc cggtccggtg accggaagcc cccgcactcg tgtccaccac acctcgccgg
      361 gcctgacggc ccatcccacc agcacggcgc cctctttatg cgacaacgcg catagtcgcg
      421 atcccgcatg gacgcccccg gaccgcgccc gtaccttcat ggtcaacagg gcccgtcctg
      481 ggccctccat ccccgggggg ttcgtgcatg gctcgcgaag aattacagcg gttgaccggc
      541 gacaacggaa acggcggccc ctgtgggcaa ggggactgtc ccaacgtcta ccgcacctct
      601 tccggctcca tcgtcgtaca aggcgatgtt tcggacgcat tcaccccgcc gcccggtgag
      661 ggcttggtcg aaatcccgga ggctgtacta cgggaggcta tccatgctct tggctggtga
      721 cgagtgggcg gccaagttcc gtgacttccg atcggaagca tggcgcctgg aaacgctgcc
      781 tcagtatctc gtcccgcagg aagcggacga gttccaagcg ttcaaggaag gggcccgcta
      841 tccgggcccg tacgaggacg actggatgcg gatggtccgc acccgaaccg tggggcgagt
      901 acacatcgtg cgccgcccgc tgtccgcgta cctggagttc gagttcgacc ggtattatcg
      961 acatcaggct ccggccggtg aagacattcg catcctcgac gtgaccgacc gccccaaccc
     1021 tctcccggac acattgagcg acttctggat gttcgaccga tccaccgtcg tactcatgaa
     1081 ttacgaggag gacgggacgc agatcaaccg gcagctggcc gaaggggatg tgtcaccatt
     1141 cctcgcatac cagcggatcg ctgtggccga atcggtgccg ttcgaggagt acgtccgcgg
     1201 tggccatcga gcctgatgcc ctcgaccgga ccggcctgga gcttgctgcc atgctcaggc
     1261 aactgcgtaa acaagccggg ctctcagggg cccggcttgc cgcacgctgc aacatgtcac
     1321 agtccaaagt cagccggatc gaggggcacc gggtccgccc gtctcttgtg gacgtggagc
     1381 ggatcctcaa agggctcggg gcatcccccg cggtagccgc cgaagccatg gccatggcgc
     1441 gcatagctca gacggagtgg caggaccaca aggcgctgca ccgtaagggg ttggacaaga
     1501 agcaacgtga gctggccggt cttgaagccg cgaccaccga tttccggttc tttctgctgt
     1561 ccatggtcac aggtctgctt tcgaccccgg agtacatccg agcaagcatc cttgacgctc
     1621 cgccgacgca gcaagctaga gcggtggcca tgaagctgga acgccaagcg gtactgctcg
     1681 accgctccaa gcggttcact tttatcctca cggaacaggc ggtcaggtat ccactggtcg
     1741 cacccgacgc catggccatg cagatcgacc gcatatcctc actcgcccgt caaaccaaca
     1801 tcagcattct gctgcttcca ctgtcggccc gatacagcaa tacacctttg tccacgttca
     1861 ccgtgtacga cgaccgtctg gccactatcg agacggacct cggctcggcg gtattccgcg
     1921 acccgaggga cattcgctcc ctcctcgacc ggttcgcgtt ctacgaagac caggccgtca
     1981 cagacgaggc ggtacaggag ttgttgagcc agtgggcctg gtcattcagg aactgatctt
     2041 tagtccacca cgcgcataag ccgtgacggc ctccgccctc aggggattac ctccatgagt
     2101 catccgcccc gatcccctgt cgcggcggcg gcccggtccg gcctcgggcc cggccaaccg
     2161 atcaggaagg tatgcccatg atcgacggca agaagctgta cgcactcgac atcagcagcg
     2221 cccggttcgc gaaggcgtgc ggcggcaaca cccatccgga cggggagtcc tgcgtcaccc
     2281 tcgcccgcat cggtgacgac gcgtgggccc tgggcgactc caagcgcccc gacgcgaccc
     2341 cgctcaggtt caccacggcc gagttggaag cggccggcat cacccccgtc gcgttcggcc
     2401 tggcctgacc cggccgccga ctctggctgt cgggagggct gttgcatcac cacgggtacc
     2461 tctggaccgg ttcacggcaa gccttcgacc gggaaggcga caggcgtccg cccgcggacc
     2521 gtccgccacc cccgccgacg ggcgaggacg acaaggacgg gcaccgtctg attcggcggt
     2581 accgggaggc cgcggcagaa ttccggacgg gcagcctgcc accgattcgg atcgcgcact
     2641 ggttgctgaa accggcaggt ctcgtatgcg gcacatggaa ggagccgaag gaagcggcgg
     2701 cctggctcga cgacaggttg agggagtacg cgtcccggtt cgactcccac caggagcagg
     2761 aaccggacta cctgtccaca cgcgtgaacg gggcgttgga gagattgtca tggggtggtg
     2821 acgtgtcgtg ggggatctat ctcgcacggc cgtcgtactt gtccttggct gtcgtgacct
     2881 gttcaccgaa cagagctgaa ccgcacacac cgtgccccgt cggggcgtag gtggccgggc
     2941 tcctcggcag gcccggccct cacttgaggg ccgggccctt cgcatggtcg cgccgggcgt
     3001 gcggacgatt ccaatgagcg atggccgtgc ccggcgggcg gtacgccccg tggtgggtcc
     3061 ggtccggcca ggcgcacgcc cggttcacac ccgccgcggt cagctgccgg ctcacccggc
     3121 gacggcgatc gtgccctggg ccaccgcgca gagggtttcc acccctgcgg cgtccaccat
     3181 cagcaggtca cagcggcaga cgacccgggt gcggccgggc cggaccacct gcgcccgtgc
     3241 gcgcagcacc tcgccccggg ccgggcgcag atagtcgatg gtgaagccgg ccgtcaggac
     3301 ggacgggccg accgccgcgc ccgccgcgaa ggtcagggcg ttgtcggccg cgtagctcag
     3361 gacgcctccg tggacgaagc cgttctgctg gcgcagctcg tcccgtacgt ccagctccag
     3421 gaccgccgct ccgtcgccga aggccgtcag ccgtgcgccc agcaggacgc tgaagggctg
     3481 ggcctccaac accttctgcc cgaacgcgag ttccgaggac tgtgcctcca tgccgcacct
     3541 ctctctcctg gtggggtgag cgtcgcctcc cgggagcgtt ccgtcaacgt cggccctgcg
     3601 gagccccgtc gacgccgggc cactagtgtc tgccggatgg agaacatgat cccccggggc
     3661 aactggctcg acgacgacat cttccgcaag ctggtgcgtg aggtcacccc gctccgcttc
     3721 ggctcgtgga gcctgcggga gttcgagcac gccacctcgg agctgggctg ggaactccgg
     3781 gagcccaggg aggcggcggg ccgggtccgg cgcagattcg ctccccggaa ggggccctgg
     3841 ggcggctacg gcacggtgat ctccgacgcc tcggaacccg accgggtgcg caagctgaac
     3901 gtacgcgtcg tcgacctgcc cgccgaggac atggccaccg ccgcgggctt cgttcgcgcc
     3961 gcctggtggg tcatggagga ggagctgggc ccgcccacga tgtggggagg cgactccggc
     4021 ccatggatgc tgtggcgcca ccccggcgcc tgcctcctcg tgcactcgca cgacgagggc
     4081 gaggtgagcc tcgaactgct gcctcccgac gctgacacgg acgccgccgg aaacggctac
     4141 tcccgtggcc gctggcgtgc cgccgagcag gcagacctgc cgaccgtgcg gcccgcttcc
     4201 gacaccacct gggaagaggt ggagaagcgc ctgtccgaaa ccctgcgctc cctcgaccac
     4261 gacaccccgt tcttccccgg caggttcatc ctccacctgg gatccgccga cgacccccag
     4321 cggttcgtcc agtgctggag ccaggatcgg ggcctcgtcg tcgaagccac cggctacctg
     4381 caccggccgg aggccgtcga cgccacccgg ctggcggaca acggctggga ctcctcccgc
     4441 tcggtctggc aacgccgctt ccccgacgcc atggaccagc ccgcgcacgc cgcgacgggg
     4501 gcccgcatgc tcgtcgagga actgcggcac ctgggcgtcg acccggccga cctctcctac
     4561 gacggaacca tgaccggccg cgggcgcggc ttccacctcg aactgccgga cctgggaatc
     4621 ccccgcgtcc accatcccgc cgagtgacac cggatcgcgg tctcgcaatc aacgcgccgg
     4681 tccgggaggg tcaccggctt ctacttccgg ccatgcctgg atgatcgttc cgacgggcga
     4741 cagcgcgccg gataccgaag atccttcaca ggtagggcac atacacggca agagctgcgc
     4801 cttccggccc ctacggcacc ggcgcggagc ggcacgcggg gccgaggaag ccccgatgac
     4861 cgtgcatacg tgtccatatg agagccaacc ccttttcgcc tccttatgcg gagagtcacc
     4921 cccgtgtaca catgatcaac ggcggccccg aacagcctgg ggggacgcct cagccatgcg
     4981 tatttggggg tacatccgtc atgcccggac ccacccgtcg tcaggccctc ggggccgcga
     5041 tcggcgtcgc cgcgggaggc gcgctcgtcg gcgtcacctc cgcgtccgcg gacaccgtcc
     5101 cagcaggaac cggcagcgaa ggcagcgcgg ccggcctcgc cgggaccccc ggctccttcg
     5161 acgaggtctt cgagggccgc cgtatccagg gcacgcccgc cgaggcccac gcgagcgccc
     5221 acgcacacgg gcacgccggg cacggcgccg gctaccgcgt gctgatcgac ggccgtgaac
     5281 tccccgtgat gcagcacggg aagagcggct ggagcagcac gatcaaccac tacgagagat
     5341 tcgcgacccc gctggaggcc gctcgtaccg cggtcatctc gctcaagggc gccgtcgtcg
     5401 ttccgttcga ccccaccgcc tgagccggga gcagcagcca tgaccgtacg gaagaatcag
     5461 gcgaacctca cggcgcccga gaagaaggcc ttcgtcgacg cggtactgga ggtcaaacgc
     5521 ctgggcgtgt acgacgaatt cgtcctcgcc caccgcacac ggttctccat agacatgaac
     5581 accggtgtgt acgtgggcca cttcgggccg tcgttcctgc cctggcaccg caagttcctc
     5641 atcgacttcg aggccgaact ccagaaaatc gacccgtcgg tctcgatccc ctactgggac
     5701 tggaccgccg acaacaccgt cggctcctcg ctctgggccc cggacttcct cggtggcacc
     5761 ggacgtcccc tggacgacca ggtgatggac gggcccttcg cgcattccgc cgggaactgg
     5821 acgatcaccg tctcggtcga cagccgccct ttcctcgccc gcaacctcgc ccatcgcgtt
     5881 cccacgcttc cgccgcggtc cgacgtcgac gccgccctcg ccctgcccac ctacgactcc
     5941 ccgcccttcc gtgacggctc ggcgggcttc cgctccgcac tggagggctc cgcgggctac
     6001 atgagcatgc acaaccgggt gcacacctgg gtctggggcc acatggagac gagcgtctcc
     6061 ccgaacgatc cggtgttctg gctgcaccac gcgttcgtcg acaagctctg ggccgactgg
     6121 cacgcgcttc acccgacgag cgccgaatac ctccccgccg ggggtacgac cgatgtgatc
     6181 gacctgaacg acaccatgcc gccctggccg aacacgaccc cgcaggacat gctggaccac
     6241 acgctgttct atacgtacgc ctgagtcctc gtcacgcccc gcgtgacacc caggcatccc
     6301 tgcggaccgg ccgaccgggg tggaggcggt ccgcagggat ggttcgtgaa ccccggaacg
     6361 cggtccggcc atctgttgca gtccgtacgg accaggcaca gggggttggc ggccctacct
     6421 gccgcgccaa ctcacgaact cacggtagtc ggccgggaac accaggccgg tagtccgctc
     6481 gatctgctcc cagtccaccg cgtcgccgcc ggaactgggc ggcggaagca gctcgcgcag
     6541 gcggacgaca gcggggtggg gcatacgggt tccttctgct tgcagggggc ggccaggctt
     6601 accgctgccc cgatagcgga acgtcggcgc cggtgcggct gtcgacggcg ggcttcgggt
     6661 cggggccgtt gcccacgttc tggtcgatcg gcggcctcgg cgggacgacg acgcagcgag
     6721 tgaggcagcc gccacgagca cccccgccag cagtagacgg ggcagcagcc gtgcccgttc
     6781 ccggcccgcc ctatgcggtg gagtcccgat ttgccgcgcg gctgcttcac accgcgtccg
     6841 ccgccccttc ctcagcgagg cccccttcct cccgtgtggg aagcgcgcct gcggtacacc
     6901 cacgtcggag ctgtccgtcg gctcaacaac accgttcggt ggcctcgttg gtcatccacc
     6961 ctgccgctgt cgacctgccg catgcactca tggagtaggt cacgatgctg atcgtcaccc
     7021 gtgagggcga ccgccgctgc aagctccacc cgtcacagcg cgcgatggtg gcactggtgg
     7081 tgtacctgcg cgagcacgaa cccgacttcg tcctcctcga cggcaccctc gccgaatgcg
     7141 accgggtcgg cgaaggccgg gccgactggc cgcagcacga gcgcccgtcg aacgcggggc
     7201 cgcaagactg aagtcctggc agatcttccg cagatcccga tgcagcccca acctcatgac
     7261 gtcaatcgcc aaagcggtcc tcgtactgga gcggcaacgc tgaagaagct cactgacgcg
     7321 gtgctccatc cgtcagatcc tggaactggg agtgcaggtg agctgtgccc cgctacccgg
     7381 cacggtccgt cggaggcttg cagcaggcgt gttgccgtgt cgctggtcct gtgcggtgct
     7441 tcgtcatcct gcgcgaatga ctcgcacctt gcgaacaaaa caagccctcg tctggcgtgg
     7501 accgatccat aggcctcttt tcggcattca tttttcggcc cgccctctta cgtggaattc
     7561 cttacgcgtg gcgctcctca cgtcattcga gttggatcaa gccatcctga tcccatcggc
     7621 cactcgttcg gcccgggact ttgacttaaa tgcaggtttc cgtcggccag cagggatgag
     7681 cggagtggaa tgctccatca acgagccagc gtttcggtga ccgcttgccc tctttgtctg
     7741 ttttttacct ccttcggtcc atcgctggag gtgctgaggc aagggtgtta tttggaagcc
     7801 aatgcatacg ccaactgcgt ggacaacttg atcccgctcg tgaaacggtc ttgcctgtcg
     7861 ggaaaacagc aggaattccg caatcggttc gctgcgaagt ggaacgacgg tttccgcgaa
     7921 aggaagtcat cgttctccag gccagggcgc tcgcatgtgc ggtgttgcat gcgcattgca
     7981 tagcgagtcg ccatgttgaa ttcttccgac gcgaagagtg tcagtcgtac atcaagcgtc
     8041 ctccggcatt gaccagaggc gtgaaggaga gaagatgaag aagttcgcta cagccgctgc
     8101 catcgctatg ggcggtctcg ccctcgtgat cagcactcag ggctccgcgc aggcctcggc
     8161 tccggccgcc caggcctccg cccagacttc cgcccagcag accgatgacg tccgcccgca
     8221 ggcgctcggc ggactcgtca aggcggcagg caaggcggcc ggtaaggcgt acgtgcatgc
     8281 caaggccgct tgcccgtccg tcgggaaggc cgccggcgag gtcgcgggca acttcctcgg
     8341 cgggtccgtc gcgccggcgg acaagatcgg caacgccgaa gcgatcgaca cggtcttcga
     8401 caagtgacac aacaccttat ggtccggagg gtggcgctga tcatcggcgc tgccctcctg
     8461 ggcatctact tcctgtttgc ttccttctcg cagatgcctc tgactccgat gaaggtgaag
     8521 tactacagcc aggtcaacga cgttatgcag ccctatatgg ctcagaactg gatgctgttt
     8581 gccccggacc ctctttcgga tgaacgtgga attctctccc gcgcccgatg cgccgacgga
     8641 acagcgaccg atttctacga tgtcaccggc ccctacgtcc agaaggtcca gggtgaccgc
     8701 ttcttcccgt cacgcatcaa ccgtctggtc agcgggaccg tcgcccagct ggacagcagc
     8761 gacccggtcc ttcagcgact gcgggacacg gaaaaggagg cgaagaagaa gcaactcccc
     8821 cttctgcctt acgagaagac gtcccgggaa caggcagaga ggttcctggg ccggtactcc
     8881 ctgacgcaaa tgccgggtgt gtgcgccggc cgtcccgctg ctgtccaggt acgtgtctac
     8941 gtgcatgaac tgccgccgtg gtcccagcgg aaggatgaat ccgccaaggg gtccaccaag
     9001 tatcaggacc tgtcctggag agaggttgac accctcaaat gaggaagatc cttgaaaagc
     9061 ttgacgggtg gacgttgagt ccggtcagcg tggtcggtgt atccggtgcc cgagttcttc
     9121 tcggattcgt cggtctcatg tactacgtca gccagtacgg tgaccgtgcg ttcctcttcg
     9181 gtccggacag cgttctcccg tggcaggagt tcgctgaccg agtcgcagag aacgactctt
     9241 tcagtcttta tgccatcagc tcttccagtg cctggttcga gattgttttc catcttggca
     9301 tcatcatcgc gcttctggtc atgctgggag taggggggcg tgtaggcctc gccgttcact
     9361 ggattttcct gtggtctata tatgaacgtc agacggtact gctggacggc ggggacaacc
     9421 ttgcctacct cgtcattccc atgcttctcc tgacgcgctg ttatgaccgc ttctcatttc
     9481 ccagtggaat aggcgcaaaa cttgcgtccc gcctgcctga cagggttcgc gtaacggcga
     9541 cgccgctgca caacctgggc gtgattgcca tcgcgcttca gatgtgcctg gtctatgtgg
     9601 tcagcggcct ctacaaagtg caggggcaga tgtggcagga cggcaccgct ctcttctatg
     9661 tcctgagagt gccagagttc agcttcccgg cactgtcgca cctggtgtac gagaacgatt
     9721 tcctggtctt ctctgggaca tatgtgacag ttctgttcct cgtctacttc cctctggggg
     9781 ttttggtacc tgccctgcgg ccgtgggccg cggtcatgtc cattggtttc cacctcggga
     9841 tcggattgct catggggctg accgggtttg ccttgaccat ggtggcctgc gatctggtct
     9901 tcctgagcag agggctggac gaggccgtca cctacgcgac cacgcggttg caaaaggccc
     9961 gctcccgcag actcgaaaag cccgcggccc aaggctcaca cgacgggaac acggccggca
    10021 gcaggcaggt gaaggaggac cacacctccc cggagccggt cggctccgga aaagcggggt
    10081 agccgaccgg cttggccccg aacctgtccc tggtgccgcc tccacctcct gcatggccag
    10141 aatgaggcca cacagccaga aggactgctt agtgtcggtc aaagtaccgg acagaatgct
    10201 gcttcaccgg ccgcgcgctg tgggttccgg tcggcgatga gagaggcagc caacgtccgg
    10261 tgtcctacag acagcgctcc ggtgccgaca gacgacacct tgcgtctgtg accgagaaac
    10321 ggagctggaa atggaagtgc catcgcccga ggacgagcgg ctcaaccggc aagggtgcat
    10381 gatcggcctg ttctggttgg aacaggacaa ggtgtgcctg ggg
//
