LOCUS       NZ_KZ195572            11392 bp    DNA     linear   CON 06-SEP-2024
DEFINITION  Streptomyces sp. CS057 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ195572
VERSION     NZ_KZ195572.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS057
  ORGANISM  Streptomyces sp. CS057
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 265.32x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188345.1-RS_2024_09_06
            Annotation Date                   :: 09/06/2024 02:40:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,152
            CDSs (total)                      :: 7,074
            Genes (coding)                    :: 6,991
            CDSs (with protein)               :: 6,991
            Genes (RNA)                       :: 78
            rRNAs                             :: 10, 6 (16S, 23S)
            complete rRNAs                    :: 10, 6 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 23 of 83
            Pseudo Genes (incomplete)         :: 71 of 83
            Pseudo Genes (internal stop)      :: 4 of 83
            Pseudo Genes (multiple problems)  :: 14 of 83
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:25:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1663067
            Orig. end                         :: 1674459
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195572.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
FEATURES             Location/Qualifiers
     region          1..11392
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="13"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11392
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11392
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6392](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6392
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1429..1644)
                     /locus_tag="B9W61_RS06835"
                     /old_locus_tag="B9W61_06835"
     CDS             complement(1429..1644)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452201.1"
                     /locus_tag="B9W61_RS06835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_06835"
                     /product="hypothetical protein"
                     /protein_id="WP_087764523.1"
                     /transl_table=11
                     /translation="MAVDTDTDADGTYCETPGPIAPTPPGGAAEGPPYAECVLCREPTE
                     YPESTKGATLCPVCAWQEAGRTACSG"
     gene            complement(1653..2321)
                     /locus_tag="B9W61_RS06840"
                     /old_locus_tag="B9W61_06840"
     CDS             complement(1653..2321)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452202.1"
                     /locus_tag="B9W61_RS06840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_06840"
                     /product="ABC transporter"
                     /protein_id="WP_087764524.1"
                     /transl_table=11
                     /translation="MTALLRYQVALLARSQRWLAPVLLYAAVLAVGVRSGQPVLGSLGV
                     VAAAVLPVTAWAVHLCVAQEPPAARTVASAAAGPRRVHLAALLTATGCAAALAVVTGVV
                     VLLISSPVTDDGAHAVSRPSAALAGLLAVLCCVLLGAAVGALSTRPVPLDRGWSVAAAV
                     LGSLLALVITGSPARLAVTALVTGARTGTVQPSVLPLLGAAALAAAAAYLVCRIAARRG
                     "
     gene            complement(2318..3376)
                     /locus_tag="B9W61_RS06845"
                     /old_locus_tag="B9W61_06845"
     CDS             complement(2318..3376)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452203.1"
                     /locus_tag="B9W61_RS06845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_06845"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_179233721.1"
                     /transl_table=11
                     /translation="MDLRGVGHRYGLRGAWVLRGVDLALPARTLVRVEGGNGAGKSTLL
                     RLLAGIDAPAEGRITGRRARTAYVPERFPAALPLTATGYLVHLGRIHGLRSAEAKRRAG
                     EWLTRFGAADHARTPLSELSKGTSQKVAVAQALLAEPELLVLDEAWTGLDTAARAELDR
                     AVAERVAAGATVVFVDHDPRRLAGAVDLTLRAGGGGVHRVTDPAPTGPATGPQVLIEAV
                     GPPGAPLPADLPGDPARDRRPGAVPGLVRLTVPAPYSDAVLAALLTARPPWHIRQVAPA
                     ETPGPAEAPAPGVAEAPAPGPAEASAHGPTPTGPTPTGQAGSDVCAQASTQAPVHAGTP
                     ETPDAPEVSEAP"
     misc_feature    complement(3368..3370)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3379..4053)
                     /locus_tag="B9W61_RS06850"
                     /old_locus_tag="B9W61_06850"
     CDS             complement(3379..4053)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378552.1"
                     /locus_tag="B9W61_RS06850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_06850"
                     /product="AIM24 family protein"
                     /protein_id="WP_047179395.1"
                     /transl_table=11
                     /translation="MKSDLFSSDHLAQPATAPGMTLQNTKSIKYAVNGEMHARQGSMIA
                     FRGNLQFERKGQGIGGMLKRAVTGEGLALMAVRGQGEAWFAHEAANCFIVELDQGDSIT
                     INGRNVLCFDPTLSYEIKTVKGAGMVGGGLFNSVFTGYGKLGLMCEGTPIVIPVTAAQP
                     VYVDTDAVVGWSQQLTTSLNRSQSVGSMVRGGSGEAVQLMLQGEGFVIVRPSEARPEKT
                     SN"
     gene            4125..4853
                     /locus_tag="B9W61_RS06855"
                     /old_locus_tag="B9W61_06855"
     CDS             4125..4853
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072531.1"
                     /locus_tag="B9W61_RS06855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_06855"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_087764525.1"
                     /transl_table=11
                     /translation="MTSNDTPAPVPGSPFHDGPTDRDQAQQFVLPLVLHLEKTDPPART
                     DALRTAARAVLTILSDERSLGDGEWAAAMRDWQDARIRKVVRRARGAEWRKAAALDGIT
                     VTGEDGAEVRVFPPIPLDGWPKELAKLQVSGTDLDDPEPPAAPDPAGPVLWLNPDLDMS
                     AGKTMAQVGHGAQLAWWELSDAERKAWREAGFPLAVATPGAERWRELTVSGLPVVRDAG
                     FTEIAPGSCTVVADHPALRR"
     gene            5001..6392
                     /locus_tag="B9W61_RS06860"
                     /old_locus_tag="B9W61_06860"
     CDS             5001..6392
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100703.1"
                     /locus_tag="B9W61_RS06860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_06860"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_087764526.1"
                     /sec_met_domain="DUF692 (E-value: 2.8e-91, bitscore: 304.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELGIGIGWRPEIAAEVESLSGIDWVEAVAENLCADHLPDSLVRL
                     RERGVTVVPHGVALGLGGADRPDPDRLAGLAARAELLGAPLVTEHIAFVRAGGPLTASP
                     RLEAGHLLPVPRTWDALDVLCENVRIAQDSLPVPLALENIAALITWPGEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNLGQDPAKALDELPVEAIAYVHVAGGVEKNGVWHDTHA
                     HPVSEPVLEVLAELRSRVDPPGVLLERDDAFPPGAELAGELDAIRATLRRAAPRVDPRP
                     APRGAAGGRGADEAGGGDGGGGRAPASDDSAGDGAGARTAAPAPVPAGTRDRTAVAQTA
                     LLSALVAGTPAPEGFDHRRLRVQSRALAAKRADVVAKVAPELPEILGDGYRAAFLAYAG
                     SRPMSGGYRRDALDFAEHVLIAGGPADPAARRRLTYWWQDRSGARPPGRTTRLVRAARA
                     VLVGK"
     gene            6398..7462
                     /locus_tag="B9W61_RS06865"
                     /old_locus_tag="B9W61_06865"
     CDS             6398..7462
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072528.1"
                     /locus_tag="B9W61_RS06865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_06865"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_087764527.1"
                     /transl_table=11
                     /translation="MNTIALSATLAVVVSSVLLVAKAVAERSRRPSPGPGVALHDLYEV
                     AFLNGGPARVVDTALTALHADGRLAVGGPGIVAVQRAQANDPVERAVFQELAAAPNGAL
                     HVLREAVMRHPAVQEVGDGLAARGLLVEPHRLRPLRQWGLTQGIACVIAVPLSLLLTFV
                     QFATDDSPDFSVPFIMKMLPAIVIGSFTGMIVANSSAGRLTASGRHAAQSFRAAYAYVV
                     TPDHLVATLGLRALPDPVFQAQLTAAARLTAPRRRSSPRFTGSTSTGVTAVGAATVWCA
                     ASVPGGSSCGGSTGGGSGSGCGGGSGCSSGSGCGGSGGSGSSCGSSGGSSCGSSGGSSC
                     GGGGGGSSCGGGGS"
     misc_feature    complement(6399..6413)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.1"
                     /tool="antismash"
     gene            7611..9173
                     /locus_tag="B9W61_RS06870"
                     /old_locus_tag="B9W61_06870"
     CDS             7611..9173
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (101-476). E-value:
                     9.6e-20. Score: 63.2. Matches aSDomain:
                     nrpspksdomains_B9W61_RS06870_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956907.1"
                     /locus_tag="B9W61_RS06870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_06870"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_087764528.1"
                     /sec_met_domain="PF00561 (E-value: 6.6e-25, bitscore: 86.9,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTAALYTSIGSLVLSALAAAPAGAWEGRGSAEARGTAIAAARAT
                     AAGIDFVSCPEKEMLPDSLKCGTVKVPLDYANPDGKQLELTVSRTPATGPAAERQGAFV
                     YNPGGPGASSITFPMAGELPEWKEIAEAYDLVGYAPRGVNGSSAPLSCQDPVAYTKGPT
                     DAPTHPTQEYKERRVARAQAYAEGCATHAGETLRHYTSLNNARDLDVLRAALGEPRLTF
                     MGASYGTYFGALYATLFPSHVRRMVFDSAVDPDPEQIWYRSNMGQSLAFEARWEDFRRW
                     VAKHDDVYHLGTTPEAVQERYDEVRAELAVKPAGGKVGPGQLHSAFLGAGYYDDYWAMR
                     ATALSEYVRGNPEPLVAQASPRAVAANDNENAQAVYTAVECNDAPWPEDFEVWDRDHTE
                     LARVAPFETWDNAFSNLPCAFWPAPRQRPLEVGTRWGELPPVLILAAERDAATPYKGAL
                     ELRKRLPGSSLVTERDAGTHGIGGAGNTCVDDHLRRYLLTGEVPGRGADCAAHPEPNPV
                     SLD"
     aSDomain        7914..9038
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9W61_RS06870_Abhydrolase_1.1"
                     /evalue="9.60E-20"
                     /label="B9W61_RS06870_Abhydrolase_1.1"
                     /locus_tag="B9W61_RS06870"
                     /protein_end="476"
                     /protein_start="101"
                     /score="63.2"
                     /tool="antismash"
                     /translation="AFVYNPGGPGASSITFPMAGELPEWKEIAEAYDLVGYAPRGVNGS
                     SAPLSCQDPVAYTKGPTDAPTHPTQEYKERRVARAQAYAEGCATHAGETLRHYTSLNNA
                     RDLDVLRAALGEPRLTFMGASYGTYFGALYATLFPSHVRRMVFDSAVDPDPEQIWYRSN
                     MGQSLAFEARWEDFRRWVAKHDDVYHLGTTPEAVQERYDEVRAELAVKPAGGKVGPGQL
                     HSAFLGAGYYDDYWAMRATALSEYVRGNPEPLVAQASPRAVAANDNENAQAVYTAVECN
                     DAPWPEDFEVWDRDHTELARVAPFETWDNAFSNLPCAFWPAPRQRPLEVGTRWGELPPV
                     LILAAERDAATPYKGALELRKRLPGSSLVTERDAG"
     gene            complement(9333..10061)
                     /gene="hemQ"
                     /locus_tag="B9W61_RS06875"
                     /old_locus_tag="B9W61_06875"
     CDS             complement(9333..10061)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965515.1"
                     /locus_tag="B9W61_RS06875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_06875"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_047179391.1"
                     /transl_table=11
                     /translation="MSAPETVTSSKSPNAGKKAKDLNEVIRYTLWSVFKLRDVLPLDRA
                     GYADEVQELFDRLAAKDITVRGTYDLSGLRADADIMIWWHAETSDELQEAYNLFRRTKL
                     GRALEPVWSNMALHRPAEFNKSHIPAFLADETPRDYISVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARLHVRE
                     EVPFYTGRRKSVADLVAGLA"
     misc_feature    10496..10512
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.3"
                     /tool="antismash"
     misc_feature    complement(11048..11063)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.74"
                     /tool="antismash"
ORIGIN
        1 ggggtccggc tccggggttc gagcacttca gggtgcccga gacgctgttg ccccggacgg
       61 ggaaggtgcg ctgcgggctg gtgcactcgc ccgcgggttt cccgtcgatg gtgaacgtgg
      121 cactcatcac cgcggtgacc tcacccttgg tgacgcgttc ccgccgggcg atggaggtga
      181 cctcgccggt gaagttctgg gtggcggagc agccgtccgg gctgcagtcc atctctcccg
      241 cgccgtccag ggtgaaggtg atgccccggt cggtcgcggc ctccaactgg tcggcgtact
      301 cgaccagggt gtcgaacatc ttgtcggcgg cgtcggcgag caccggggtc aggtccatcc
      361 cctccgcgtc accggatgcc agaggcccgg tggtcaccgt gcggggagcc gtcggctcct
      421 ctccgtcctc gatccggtcc ctcaggtcgg cgaggccgtc gcggaggtcg tacgcctcca
      481 gccgcagcac ccggtgcggc ttctcctccg tcaccagcag gcggccggcc gaggtgtcga
      541 taccgagcgc gggcgttccg ttgacgctca gtggccgctg cccggacgtg ctcgacgcct
      601 tcggcccgtt cacgcgctgc ggcgggttgt ccgggtccgc caaggccctg tccagcacgt
      661 ccgccagttt cgagggggcg gccgtgcggg ccagcgcctc gtccagcagc tcggagccgt
      721 cgtcgagccc cacgatccac tcgctcggcg gcgccgtctc accggccgcc acgtccttcc
      781 tgggggcggg gtcgacctgc caccgggtga acttcctgcc gccgatgcgc agcacgtccc
      841 ggccgctgtc ggcgccgccg ccggaggaga cggtgccgaa ctggctgccg gcggcgctga
      901 cggtgatctc gttctcggtg ataccgaggg ccgaggtgtc cttgtagctg aggccgggcg
      961 cgatggccag gtcgtagacg gcttccctga acggcttgag gttctgctgc gccgcctgcg
     1021 actcgtcgga atcggagggc gaggacgtca gcgacaggag cccggccacc agccccacgg
     1081 agacgaggcc gatcccaccg gccagcagcg cccgtcggcg tccggtcagg gtccggatcc
     1141 gcgccgtcca cggaaggccg ggcggcctgc ccgcgggcct gggagggccg gccggtgggg
     1201 cgctcggccc gtccgccgcg gggccgctct caggttcttc gtcgctcggt cctcgtccgg
     1261 tcatgactcc ccctcctgcg catccatgac acgtactcac ggggccctga gatccgtcgc
     1321 atgccgacag accgcccccg tccaagcacg gagaatacga gcagtcacct atcaactacg
     1381 gtgctttgag ctggagttca cagaatccga ccatcacccg gcctggctct aacccgagca
     1441 ggccgtgcgg cccgcctcct gccaggcgca gaccgggcag agcgtggcgc ccttcgtcga
     1501 ctccgggtat tcggtcggct cgcggcacag cacgcactcc gcgtacggcg ggccttcggc
     1561 cgccccgccc ggcggggtcg gtgcgatcgg ccccggagtc tcgcagtacg tgccgtccgc
     1621 gtccgtgtcg gtgtccacgg ccacgagcct actcacccgc gcctggcggc gatccggcag
     1681 acgaggtacg ccgccgccgc agcgagggcc gccgccccga gcaggggcag gacggacggc
     1741 tggaccgtcc cggtccgggc tcccgtgacc agggcggtca ccgcgagacg cgccggtgat
     1801 ccggtgatca cgagcgcgag gagcgagccg agcacggccg ccgcgacgga ccagccccgg
     1861 tcgagcggca ccggacgggt gctgagcgcc cctacggccg cgcccagcag cacacagcac
     1921 aggacggcga gcagtccggc cagcgcggcg gacggccggg agacggcgtg ggcgccgtcg
     1981 tcggtgaccg ggctgctgat gagcaggacg accacgccgg tcaccacggc gagcgccgcc
     2041 gcacagcccg tggcggtcag cagcgcggcc aggtggaccc gccggggtcc cgcggcggcc
     2101 gaggccaccg tccgggcggc gggcggctcc tgggcgacgc agaggtgtac ggcccacgcg
     2161 gtgacgggca gcacggcggc ggccaccacg ccgagcgagc ccagcacggg ctgcccgctc
     2221 cgtacgccga cggcgaggac ggccgcgtac agcaggacgg gtgccagcca gcgctgggaa
     2281 cgggcgagca gggctacctg gtagcggagg agggcggtca cggcgcttcg gacacctcgg
     2341 gggcatcggg agtctcgggg gtcccggcgt gtacgggagc ctgcgtggac gcctgcgcgc
     2401 acacgtcgct accggcctgc ccggtcggcg tcggcccggt cggcgtcggc ccgtgcgccg
     2461 atgcctcggc cggcccgggt gccggtgctt cggcgacccc gggcgccggt gcctcggccg
     2521 gcccgggcgt ttccgccggg gcgacctgtc ggatgtgcca gggcgggcgg gccgtgagca
     2581 gggccgccag gacggcgtcg gagtacgggg cggggacggt gaggcgtacg aggccgggga
     2641 ccgcgccggg ccgccggtcc cgggccgggt cgcccggcag atccgcgggc agcggtgcgc
     2701 ccggcgggcc cacggcctcg atcaggacct gggggccggt ggcgggtcct gtcggcgccg
     2761 gatccgtcac ccggtgcacc ccgccgcccc cggcccgcag ggtgaggtcc accgccccgg
     2821 cgaggcgtcg tgggtcgtgg tcgacgaaga ccacggtggc gcccgccgcg acgcgttccg
     2881 ccacggcccg gtcgagttcg gcgcgggcgg cggtgtcgag accggtccag gcctcgtcca
     2941 ggaccagcag ctccggttcg gcgagcagtg cctgggcgac ggcgaccttc tggctggtgc
     3001 ccttggagag ttcggagagc ggggtacggg cgtggtccgc cgccccgaac cgggtcagcc
     3061 actcccccgc ccgccgcttc gcctcggcgc tccgcagtcc gtggatccgg ccgaggtgga
     3121 ccaggtagcc ggtggcggtg aggggcagcg ccgcggggaa gcgctcgggg acgtacgccg
     3181 tgcgggcccg acggccggtg atccggccct cggcgggggc gtcgatcccg gcgagcagcc
     3241 gcagcagggt cgacttcccg gcgccgttgc ccccttcgac ccggacgagg gtccgcgcgg
     3301 gcagcgccag gtcgaccccg cgcagcaccc aggcgccgcg gaggccgtac cggtggccca
     3361 cgccccttaa gtccacggtc agttggaggt cttctcgggc ctggcctcgc tgggccgcac
     3421 gatgacgaaa ccctcgccct ggagcatcag ctggacggcc tcgccggatc cgccgcgcac
     3481 catcgagccg acgctctgcg agcggttcag gctggtcgtg agctgctggc tccagccgac
     3541 gaccgcgtcc gtgtccacgt acaccggctg ggcggcggtc acggggatca cgatcggggt
     3601 gccctcgcac atcaggccga gcttgccgta gcccgtgaag acgctgttga agaggccgcc
     3661 gccgaccatc ccggcgccct tcaccgtctt gatctcgtag gagagcgtgg ggtcgaaaca
     3721 gaggacgttg cggccgttga tggtgatcga gtcgccctgg tccagctcca cgatgaagca
     3781 gttggccgcc tcgtgcgcga accacgcctc gccctggccc cggacggcca tgagggccag
     3841 gccctcgccg gtgaccgcac gcttgagcat gccaccgatg ccctggccct tgcgttcgaa
     3901 ctggagattg ccccggaaag cgatcatcga gccctgccgg gcgtgcatct cgccgttgac
     3961 ggcgtacttg atcgatttgg tgttctgcag ggtcatgccg ggggcggtgg ccggctgggc
     4021 caggtggtcg ctggaaaaga gatcgctctt catgcggagc atcctcaccc ggacccggtc
     4081 gttccgccaa gcaggtgacg ggttcggcct ggcagactgg gacggtgacc agcaacgaca
     4141 cccccgcccc cgtccccggg agcccgttcc acgacggtcc gaccgaccgc gaccaggctc
     4201 agcagttcgt gctgccgctg gtgctccacc tggagaagac ggacccgccc gcccgcaccg
     4261 acgcactgcg gaccgccgcc cgcgcggtgc tgacgatcct ctccgacgag cggtccctcg
     4321 gcgacggcga gtgggcggcg gcgatgcggg actggcagga cgcccggatc cgcaaggtcg
     4381 tgcgccgggc gcgcggcgcg gagtggcgca aggccgcggc gctggacggc atcacggtga
     4441 cgggcgagga cggcgcggag gtacgggtgt tccccccgat cccgctggac ggctggccca
     4501 aggagctggc caagctccag gtgtcgggca ccgacctgga cgaccccgag ccgcccgccg
     4561 cccccgatcc cgccggtccg gtgctgtggc tcaacccgga tctggacatg tcggcgggca
     4621 agacgatggc gcaggtgggg cacggggcgc agctcgcctg gtgggagctg tccgacgccg
     4681 agcgcaaggc ctggcgcgag gccgggttcc cgctcgccgt cgccaccccc ggagcggagc
     4741 gctggcggga gctgacggtg agcgggctgc cggtggtgcg ggacgcgggg ttcaccgaga
     4801 tcgccccggg gtcctgcacg gtggtcgccg accacccggc gctgcgccgc tgagcgtttc
     4861 cgcgtccgtc cgcgtatccc tggagcggcg gtacgggaac ctcaaatcca gctctgaacg
     4921 tccctccggt cgggttcatc gccgcctgac ggggccatga cccctggaca gaacgaggtg
     4981 agcaggggga cgggggtgcc atggaactgg gtatcggtat cggctggcgg ccggagatcg
     5041 cggccgaggt ggagtcgctg agcgggatcg actgggtgga ggcggtcgcg gagaacctct
     5101 gcgccgacca cctcccggac tcgctggtgc ggctccggga gcgcggggtg accgtcgtgc
     5161 cgcacggggt ggcgctcggc ctcggcggcg cggaccggcc cgaccccgac cggctcgccg
     5221 gtctcgcggc gcgcgccgag ctgctggggg cgcccctggt gaccgagcac atcgcgttcg
     5281 tacgggccgg ggggccgctg accgcgtcgc cgaggctgga ggcggggcac ctgctgccgg
     5341 tgccgcgcac ctgggacgcg ctggacgtgc tctgcgagaa cgtacggatc gcgcaggact
     5401 cgctgccggt gccgctcgcc ctggagaaca tcgcggcgct gatcacctgg ccgggcgagg
     5461 agctgaccga ggggcagttc ctcgcggagc tggtcgagcg gaccggggtg cggctgctga
     5521 tcgacgtggc caacctgcac accaaccacg tcaacctcgg ccaggacccg gcgaaggcgc
     5581 tggacgagct gccggtggaa gccatcgcgt acgtccatgt ggcgggcggg gtcgagaaga
     5641 acggcgtctg gcacgacacg cacgcccacc cggtctccga acccgtcctg gaggtgctgg
     5701 ccgagctgcg ctcccgggtc gacccgccgg gcgtgctgct ggagcgcgac gacgccttcc
     5761 cgccgggcgc ggagctggcg ggcgaactgg acgcgatccg ggcgacgctg cggcgggcgg
     5821 cgccccgggt ggacccccgt cccgcgcccc gtggcgcggc aggtggcagg ggggcggacg
     5881 aagccggggg cggtgacggt ggtgggggcc gtgcgcccgc gtccgacgac agcgcgggcg
     5941 acggcgccgg cgcccgtacg gccgctcccg cccccgtgcc ggccggaacc cgcgaccgta
     6001 ccgccgtcgc acagaccgcc ctgctctccg ccctggtcgc cggaacgccc gcgcccgagg
     6061 gcttcgacca ccggcggctg cgcgtgcaga gccgggcgct ggccgccaag cgtgccgacg
     6121 tcgtcgccaa ggtggccccc gaactgccgg agatcctcgg cgacggctac cgcgccgcgt
     6181 tcctcgcgta cgccggaagc cgcccgatgt ccggcgggta ccggcgcgac gccctggact
     6241 tcgcggagca cgtgctcatc gcgggcggtc ccgccgatcc ggcggcgcgg cgccggctga
     6301 cgtactggtg gcaggaccgt tcgggcgcgc ggcccccggg ccgtaccacc cgtctggtcc
     6361 gggcggcccg tgccgtcctc gtggggaagt gaccgccatg aacacgatcg ccctgtcggc
     6421 gaccctcgcg gtcgtcgtct cgtcggtgct gctcgtcgcc aaagcggtgg ccgagcggtc
     6481 ccgccgcccc tctccgggcc ccggcgtcgc ccttcacgac ctgtacgagg tcgcgttcct
     6541 gaacggcggt ccggccaggg tcgtggacac cgccctgacc gccctgcacg cggacggccg
     6601 gctggccgtc ggcgggcccg gcatcgtcgc cgtccagcgg gcacaggcga acgacccggt
     6661 ggaacgcgcg gtgttccagg agctggccgc cgccccgaac ggcgccctgc acgtcctgcg
     6721 ggaagcggtg atgcggcacc cggcggtcca ggaggtcggc gacgggctcg cggcgcgcgg
     6781 gctgctggtg gagccccatc ggctccgccc gttgcggcag tggggtctca cccaggggat
     6841 cgcatgcgtg atcgccgtgc cgctgagcct gctgctgacg ttcgtccagt tcgccacgga
     6901 cgactccccg gacttctcgg tcccgttcat catgaagatg ctgcccgcga tcgtgatcgg
     6961 ctcgttcacc gggatgatcg tcgccaactc ctcggcgggc cgcctcaccg cgtcggggcg
     7021 ccacgccgcc cagtcgttcc gggccgccta cgcctacgtg gtgacccccg accatctcgt
     7081 cgccaccctg ggcctgcgtg ccctgccgga cccggtgttc caggcccagt tgacggccgc
     7141 ggcacggctg accgctccgc gccgacggtc ctccccgcgc ttcaccgggt ccacttcgac
     7201 gggggtcacc gccgtcggcg cggcgacggt ctggtgcgcc gcgtccgttc ccggcggctc
     7261 cagttgcggc gggtcgaccg gaggcggctc cggatcgggc tgcggaggcg gctccgggtg
     7321 ctcctcgggg tccgggtgcg gcggcagcgg cggcagcggg tcgagctgcg gcagttccgg
     7381 cggctccagt tgcggcagct ccggcggatc cagctgtggg ggcggtggcg gcggatccag
     7441 ctgtggcggc gggggctcct gatgcggtct cccgcgacct gaatcaggtg cgtacgtacg
     7501 gttcttgagg ctccatcagg gtggattccg gcgacattcc ggggcggtgc tcgacatatc
     7561 cgtccggccg cccgactctt cctctccgca tccgcagaga ggccccgcgc gtgagaactg
     7621 ctgcgctcta cacatcgatc ggctccctgg tcctgtccgc cctcgccgcc gctcccgcgg
     7681 gagcctggga gggccggggc tccgccgagg cccggggcac cgcgatcgcc gccgcccgcg
     7741 ccacggccgc cgggatcgat ttcgtctcct gcccggagaa ggagatgctc ccggactccc
     7801 tgaagtgcgg cacggtgaag gtgcccctcg actacgcgaa cccggacggg aaacagctcg
     7861 aactgaccgt cagccgcacg cccgccaccg gtccggccgc cgaacggcag ggcgcgttcg
     7921 tctacaaccc gggcggcccc ggcgcgtcca gcatcacctt cccgatggcc ggggagctgc
     7981 cggagtggaa ggagatcgcc gaggcgtacg acctggtcgg ctacgccccg cgcggcgtga
     8041 acggctcctc cgcaccgctg agttgccagg accccgtcgc gtacacgaag ggcccgaccg
     8101 acgcgccgac ccacccgacc caggagtaca aggagcggcg cgtcgcccgt gcccaggcgt
     8161 acgcggaggg ctgcgcgacg cacgcgggcg agacgctgcg gcactacacg tcactgaaca
     8221 acgcccgtga cctggacgtc ctgcgggccg ccctcggcga gccgcgtctg acgttcatgg
     8281 gggcgtcgta cgggacgtac ttcggggcgc tgtacgcgac gctgttcccc tcccacgtgc
     8341 gccgcatggt cttcgactcg gcggtggacc ccgacccgga gcagatctgg taccgctcca
     8401 acatgggcca gtcgctggct ttcgaggcgc gctgggagga cttccgccgc tgggtcgcca
     8461 agcacgacga cgtgtaccac ctggggacca cgccggaagc ggtgcaggag cgctacgacg
     8521 aggtgcgcgc cgagctggcg gtgaagccgg ccggcggcaa ggtcggaccg gggcagctgc
     8581 actccgcgtt cctgggggcc ggctactacg acgactactg ggcgatgcgg gcgacggcgc
     8641 tgtcggagta cgtgcggggc aatccggagc ctctggtggc gcaggcgtcc ccgcgcgcgg
     8701 tggcggcgaa cgacaacgag aacgcgcagg cggtgtacac ggccgtcgag tgcaacgacg
     8761 cgccctggcc cgaggacttc gaggtctggg accgcgacca cacggagctg gcgcgcgtcg
     8821 cgcccttcga gacctgggac aacgccttct cgaacctgcc gtgcgccttc tggcccgcgc
     8881 cccggcagcg gccgctggag gtcgggacgc ggtggggcga gctgccgccg gtgctgatcc
     8941 tggcggcgga gcgggacgcg gcgaccccgt acaagggagc gctggagctg cggaagcggc
     9001 tgcccgggtc gtcgctggtg accgagcggg acgccgggac gcatggcatc ggcggggcgg
     9061 ggaacacctg cgtcgacgat catctgcgcc ggtatctgct gaccggtgag gtgccggggc
     9121 ggggtgcgga ctgcgccgcg cacccggagc cgaacccggt gtcgctggac tgatctcccg
     9181 gcggtggcgg tacggagcgg gggccgccgc accatgacgg tgcgacggcc cccgaagccg
     9241 aacccggtgt cgctggagga gcggacggca cgatcggtgc cttgggcccg cccttctgat
     9301 cttccgggtt ggggtggtgc gggactcgct ggttatgcga gcccggccac caggtctgcg
     9361 acgctcttcc tgcggcccgt gtagaacggg acctcctcgc gcacgtggag acgcgcctcg
     9421 gaggcgcgca ggtgacgcat caggtcgacg atgcggtaca gctcgtccgc ctcgaaggcg
     9481 aggatccact cgtagtcgcc cagcgagaac gaggcgacgg tgttggcgcg gacgtccggg
     9541 tagccccggg ccatcttgcc gtggtccgcg agcatacggc ggcggtcctc gtcgggcagc
     9601 aggtaccagt cgtaggaccg gacgaagggg tagacgctga tgtagtcgcg gggcgtctcg
     9661 tcggcgagga acgccgggat gtgggacttg ttgaactcgg ccggccggtg cagcgccatg
     9721 ttcgaccaga ccggttcgag ggcgcggccg agcttggtgc gcctgaagag gttgtacgcc
     9781 tcctggagct cgtccgaggt ctcggcgtgc caccagatca tgatgtccgc gtcggcccgc
     9841 agcccggaca ggtcgtaggt gccccggacg gtgatgtcct tggccgcgag ccggtcgaac
     9901 agctcctgca cctcgtcggc gtaaccggcg cggtccagcg ggaggacgtc gcgcagtttg
     9961 aagaccgacc acagggtgta gcggatgacc tcgttgaggt ccttggcctt cttgccggcg
    10021 ttcggactct tgcttgatgt cacagtctca ggagcactca tacggctatt gtcccgcctc
    10081 gctccccgtg ccccgaacca gggtcgacgt ggcgatgatc tctcccgcga tcttccccgt
    10141 gagctccccc gcgatctcgt cggccgcccg gtgcgcgctc gcgacgcagg cggggatgcc
    10201 gaccccgtcg tagaccgcgc cgcagacccg cagggcgggc agcttggcga cctcgtcgcg
    10261 gatgcgggcg acccgggaga ggtggccgac cgggtactgc ggcaggccgc cgatccagcg
    10321 ggtgacctcg gtgtccacgg gccgggcggt gaggccggtg gcggcggcga ggtcgcgcag
    10381 ggagacggcg acgagctcct cgtcctcgcg gtgcagatgg tcctcctcgc cgtaccggcc
    10441 gacggaggtg cggagcacga agagatcggg ggcggcgtcc gcgacccact gccatttgtt
    10501 gctggagaac gtggacgcct tgatcgtgtg cccgtcgacc ggcggtacga ggaagccgga
    10561 acggccgcgc agggcctcgt ggcgctcgat gtcggcccgc cggaaggcca gggtgaccag
    10621 ggccatcgag gcgtactcga cgccggacag ctcggcggac gcggccgggg actcggcggc
    10681 cagcagggtg ccggcggacc aggcgggggt ggccaggacg acaccgtcgg cggcgatcgt
    10741 ccgggcctcg gtgcggaccg cccagcccga ctccgtacgg gtcagaccga ggacgggggt
    10801 ctcggtgcgg atctcgccgc cgccggcgcg tacggcgtcg gcgacggcgg ccgggagggt
    10861 gccgatgccg cccgcgatgc cctggaagac gggtccggtc tgctgccggg cggccgcccg
    10921 ctcctggata cggcgtacgc cctccagcag cggcccgccc tccttcaccg cctcgaagag
    10981 ctggggaacg gcggcgcgca tcgagatccg gtaggcgtca ccggcgtaga ccccgccgag
    11041 gaggggctcc accagccggt ccacgacctc gcggccgagc cggtcggcga cgtacgcgcc
    11101 gaccgcgacg tcgtcgccga cgggggtcgg ggtcaggtcg cgctcctcgg cgatccgggc
    11161 caggccctcg ggggacagca cctcgccgag caccgccggg tcgccgggga cgcccatgac
    11221 gtgccccttc ggcatggggc gcagcgcgtc gcgcgtccac agcgaggcgg tggcggtggc
    11281 gggcggctgg aggcggtcgg cgaggcccac cgcctcggcc aggtcgaccg cctcggggcg
    11341 ccgggcgagc atcgactcgg ctcccaggtc gacctggacg cccgcgacct cg
//
