LOCUS       NZ_KZ195572            10798 bp    DNA     linear   CON 06-SEP-2024
DEFINITION  Streptomyces sp. CS057 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ195572
VERSION     NZ_KZ195572.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS057
  ORGANISM  Streptomyces sp. CS057
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 265.32x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188345.1-RS_2024_09_06
            Annotation Date                   :: 09/06/2024 02:40:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,152
            CDSs (total)                      :: 7,074
            Genes (coding)                    :: 6,991
            CDSs (with protein)               :: 6,991
            Genes (RNA)                       :: 78
            rRNAs                             :: 10, 6 (16S, 23S)
            complete rRNAs                    :: 10, 6 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 23 of 83
            Pseudo Genes (incomplete)         :: 71 of 83
            Pseudo Genes (internal stop)      :: 4 of 83
            Pseudo Genes (multiple problems)  :: 14 of 83
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:25:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 732056
            Orig. end                         :: 742854
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195572.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5798](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5798
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            292..825
                     /locus_tag="B9W61_RS02815"
                     /old_locus_tag="B9W61_02820"
     CDS             292..825
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964650.1"
                     /locus_tag="B9W61_RS02815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_02820"
                     /product="DUF5709 domain-containing protein"
                     /protein_id="WP_087764070.1"
                     /transl_table=11
                     /translation="MSDTDWGDDVYQPQEPEASDPGEQLDAGDTLMDPSGTGDPLDEGY
                     APPDRPWVVDGLGTTADELHHGESLEARLTREVPEPDGSAGDGVGDLVGGDGEPWDDEV
                     GVARAGRLTQQADGSVPATLTASDVGIDGAAASAEEAAMHVIPEPGDDEALLEAGPATG
                     TGQATGGTGAPSSP"
     gene            994..2439
                     /gene="gndA"
                     /locus_tag="B9W61_RS02820"
                     /old_locus_tag="B9W61_02825"
     CDS             994..2439
                     /EC_number="1.1.1.44"
                     /GO_function="GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_process="GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="gndA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100: 3-hydroxyisobutyrate dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964653.1"
                     /locus_tag="B9W61_RS02820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_02825"
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="WP_087764071.1"
                     /transl_table=11
                     /translation="MSDTAQIGVTGLAVMGRNLARNFARNGYSVALHNRTAARTRELVE
                     EFGDEGDFIATESAQDFVAALERPRRLVIMVKAGEATDAVIAEFAPLLETGDVIIDGGN
                     AHFEDTRRRERELREQGIHFVGTGISGGEEGALEGPSIMPGGSEESYDSLGPMLEKISA
                     KAKDGAPCVTHIGPDGAGHFVKMTHNGIEYADMQLIGEAYQLLRDVAGYSPAQIADIFR
                     TWNTGRLDSYLIEITAEVLSHVDASTGKPFVDVVLDQAEQKGTGRWTVQIALDLGVPVS
                     AIAEAVFARSVSGHADLRDASGHLTGPTARRLGSDEAAAFADRVEQALYASKLVAYTQG
                     FHEIAAGSERYDWNIDLGKVASIWRGGCIIRAAFLDRITSAYEARPSLPSLLADKSFAE
                     EIAAAQGDWRTVIATAVTRGVPTPAFAATLAYYDSLRAERLPAALTQGQRDYFGAHTYH
                     RTDREGTFHTLWGGDRTEVEISS"
     gene            2779..3753
                     /locus_tag="B9W61_RS02825"
                     /old_locus_tag="B9W61_02830"
     CDS             2779..3753
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1066: alpha/beta hydrolase domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122771.1"
                     /locus_tag="B9W61_RS02825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_02830"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_087764072.1"
                     /transl_table=11
                     /translation="MTDLPPPITPYLEPAAKELCEATDPHPRIYEVPPEKGRDILLGLQ
                     SDRSVPRPEVDEEWVDVDAGQWGTVRTRIIRPKGASGELPVVFYIHGAGWVFGDDRTHD
                     RLFRELAVGAGAAGVFPVYDRAPEAKYPTQVEQNYAVGQWVLEHGAQHGLDTSRIAVTG
                     ESVGGCMSAVFALMNKERGGIDLKAQVLLYPVTDADFSTPSYLQFAEGYYLTRDGMKWF
                     WDAYTGNIAHRTEAYASPLRASLEQLRGLPTTLVITDEADVLRDEGEKYANRLREAGVD
                     VTSVRVAGMVHDFLLLDSLRDTRAANVARKLAVDALRTALHDG"
     gene            3928..5001
                     /locus_tag="B9W61_RS02830"
                     /old_locus_tag="B9W61_02835"
     CDS             3928..5001
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456253.1"
                     /locus_tag="B9W61_RS02830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_02835"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_087764073.1"
                     /transl_table=11
                     /translation="MPDAVPDPVSVQPVVAPLTSAALFLVGTIEPGGESAVRGVLPDLA
                     AVARSLGFRYPDAGLACVAGFGSSAWDRLFGGPRPAHLHPFPELRGPLHHAPATPGDVL
                     LHIRAERMDICYAWAAQLLDKLGGALRIVDETHGFRYLDHRDLLGFVDGTENPVGDDAR
                     SAALVGAGDDPEFEGGSYVVVQKYLHDLTSWNALSVEEQERVIGRTKSDDVEFPDDEKP
                     ADSHIALNTITDPDGTERDILRANMPFGSFGKGEFGTYFIGYAADPDVTERMLRNMFLG
                     DPPGTHDRILDFSTAVTGSLFFAPRADFLDAPPPPPVLTSSGDRPEPVPAPVRQEPVAA
                     GLDHGSLRIGSLQESAQ"
     gene            5001..5798
                     /locus_tag="B9W61_RS02835"
                     /old_locus_tag="B9W61_02840"
     CDS             5001..5798
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955648.1"
                     /locus_tag="B9W61_RS02835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_02840"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_047179995.1"
                     /sec_met_domain="Linocin_M18 (E-value: 5.9e-89, bitscore:
                     296.2, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPVTPSAWEEIEEEARRTFRRHVAGRRVVDVSDPDGP
                     TLAAVGDGHLTDIDPPTPDVVARARTSMPVIEWRVPFTVTRQAVDDVERGSADSDWQPV
                     KDAARTCAFAEDMAIIDGYAAAGITGLRDGSSHDPLALPADARDYPVAVSQAVTRLRLA
                     GVDGPYRLLLGADAFTEAAETSDHGYPVKTHLSRLVDDEILWAPAVKGGVLLSTRGGDF
                     ELCLGQDLSIGYADHDATSVHLYFQQAFTFRMLTPEAVVGLIA"
     gene            5890..7272
                     /locus_tag="B9W61_RS02840"
                     /old_locus_tag="B9W61_02845"
     CDS             5890..7272
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264: diaminopimelate decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456256.1"
                     /locus_tag="B9W61_RS02840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_02845"
                     /product="diaminopimelate decarboxylase"
                     /protein_id="WP_087764074.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 1.3e-37, bitscore:
                     128.2, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 1.9e-08,
                     bitscore: 33.1, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQTTSAHHQGGTAPPAARFDAVVRAAVGQGLLGEGDPVAGFIDT
                     DGVRASVGALHDAFAATPEVLHTFAAKACSLVPVLRLLADCGMGCEVASPGELRLAVEA
                     GFAASRVVLDSPAKTREEIRLALALGVAVNADSLDELRRIDALRTPGTASVIGLRVNPQ
                     VGGGSIGPMSTATDTSKFGVALRDPGAREAIVEAFRERPWLTRLHTHVGSQGCPLELIA
                     DGVARAYELAEEINATLGVRRVTSLDIGGGLPVNFADESVRPGYADYVAALTAAAPGLF
                     SGRYSLVTEFGRSLLAKNGFIGARVEYTKDAGGRRIALTHAGAQTATRTVLMPDAWPLR
                     IGAFGPDGIPRTGPSLAQDIAGPCCFAGDVVAYGRELPELAEGDLVVLYDTGAYYFSTP
                     WAYNSLPRPAVYGFRAVAGPEDGSGHDGPVTFTTVRDAQSLDDIAAESGLAHADALIRR
                     DD"
     gene            7801..8571
                     /locus_tag="B9W61_RS02850"
                     /old_locus_tag="B9W61_02855"
     CDS             7801..8571
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955646.1"
                     /locus_tag="B9W61_RS02850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_02855"
                     /product="PIG-L family deacetylase"
                     /protein_id="WP_087764075.1"
                     /sec_met_domain="PIG-L (E-value: 8.5e-21, bitscore: 73.7,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLPSVLAVHAHPDDESLFCGGVLARHAAAGGRTAVVTATWAEGT
                     HRAVELAGALDALGAGAPRLLGYADARVPDSAPGRPRFLDAPLDEAVEAVVGHIRDVRP
                     EIVITVDAYGGMTGHPDHVHAHRITALAVRAAGLPRYRPASGDPWQPSALYLATHPRSA
                     AVSVGGRLARPGTPADALYCSEDAWITATVDVGPWFPQKLAAILAHRSEVERGAAPGRI
                     AVLPRAVQLEVLGTEWYIRHDLAPRGGSATELSA"
     gene            complement(8696..9049)
                     /locus_tag="B9W61_RS02855"
                     /old_locus_tag="B9W61_02860"
     CDS             complement(8696..9049)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955645.1"
                     /locus_tag="B9W61_RS02855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_02860"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_087764076.1"
                     /transl_table=11
                     /translation="MAEKAAVEIRDDREHGSLVAYEDGESAGTIAYFVMDPAPGALVAV
                     HTVVRPEHEGKGIAGALVREFYTMAAREGVPVVPLCPYAAKWAQRHPDEAPEAPAELVR
                     EAERQLRNHPELF"
     gene            complement(9111..9536)
                     /gene="panD"
                     /locus_tag="B9W61_RS02860"
                     /old_locus_tag="B9W61_02865"
     CDS             complement(9111..9536)
                     /EC_number="4.1.1.11"
                     /GO_function="GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="panD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964661.1"
                     /locus_tag="B9W61_RS02860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_02865"
                     /product="aspartate 1-decarboxylase"
                     /protein_id="WP_087764077.1"
                     /transl_table=11
                     /translation="MIRTIFKSKIHRATVTQADLHYVGSVTIDAALMEAADLLPGELVH
                     IVDIDNGARLETYVIEGERGSGVIGINGAAAHLVHPGDLVILISYAQVDDAEARALVPR
                     VVHVDADNRIVALGSDASAPVPGTRTERSPQAVVAGG"
     misc_feature    9612..9625
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.67"
                     /tool="antismash"
     misc_feature    complement(9836..9850)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.83"
                     /tool="antismash"
     misc_feature    complement(9837..9849)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.22"
                     /tool="antismash"
     gene            9849..10337
                     /locus_tag="B9W61_RS02865"
                     /old_locus_tag="B9W61_02870"
     CDS             9849..10337
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040274.4"
                     /locus_tag="B9W61_RS02865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_02870"
                     /product="DUF5959 family protein"
                     /protein_id="WP_079277217.1"
                     /transl_table=11
                     /translation="MRDVLGSGEDRSVLADERAAMGNRPPRELISLADDEGNSVSVNVL
                     GRSPGWTAGLDAEIVVKTPFVSGRIDLALYVARLESWADALDRLDAGEDVAWMEMNSGP
                     SIFIQLTGERDCPEVVVEDESGSMVTVRVPLVPPDDWVADHRRRLRQVMDHWVPMLSG"
     misc_feature    10417..10430
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.33"
                     /tool="antismash"
ORIGIN
        1 cccggcagac tcgacgaccc ttccggccct cgtacgacgg gtactcgcgg acgggtgaca
       61 ggccgatagc tccccgcgcc cgcgggaatg gtccgcggat cgccgtgccg ccgggaactc
      121 ccacgactcc ccggatgcgc ggcgtacggg ccgtgacctg cgcacggacc ggcccgtacg
      181 cccgtgccgg gcaccgaaat ccgatcttcc ctttgcggcc ggagcgggac cggagtggaa
      241 ttgcgtacag gggacagtgg tactcggtac tcccggagga aaggacgcgc catgagcgac
      301 accgactggg gggacgacgt ctaccagccc caggaacccg aggcttccga ccccggcgaa
      361 cagctcgacg ccggggacac gctcatggat ccgtccggca ccggcgaccc gctggacgag
      421 ggctacgcac cgccggaccg cccctgggtg gtcgacggcc tcggcaccac ggccgacgag
      481 ctgcaccacg gtgagtcact cgaagcgcgg ctgaccaggg aagtgcccga gccggacggt
      541 tccgccggcg acggcgtggg cgatctggtg ggcggcgacg gtgagccctg ggacgacgag
      601 gtcggcgtag cccgcgccgg acggctgacc cagcaggcgg acggaagcgt cccggccacc
      661 ctcaccgcca gcgacgtggg catcgacggg gcggcggcgt cggccgagga ggccgcgatg
      721 cacgtgatcc ccgagccggg ggacgacgaa gcgctcctgg aggccggtcc ggccaccggg
      781 accggccagg ccaccggtgg cacgggagcc ccgtcctcgc cctgagcggg ccgtcggcgg
      841 gcctccactc ctgaacgcgg ccgtccccgg gcccgtacct cccctgaccg aagcacacca
      901 cgcaccgccc gaggcaccac acgcctccga tcgcaccgga cgcaccggac gcaccggacg
      961 caccgacgta ctcggacccc caggagcaca cagatgagcg acactgctca gatcggcgtc
     1021 accggacttg ccgtcatggg acgcaacctg gcccggaact tcgcccgcaa cggatacagc
     1081 gtcgctctgc acaaccgcac ggccgcccgc acccgtgaac tcgtcgaaga gttcggcgac
     1141 gagggtgatt tcatcgcgac cgagagcgcc caggatttcg tggcggcgct ggaaaggcca
     1201 cgacgcctgg tgatcatggt gaaggccggg gaggccacgg acgcggtgat cgcggagttc
     1261 gcgcccctcc tggaaacggg cgatgtgatc atcgacggag gcaacgcgca cttcgaggac
     1321 acgcgccgcc gggagcgcga gctgcgcgag cagggcatcc acttcgtcgg taccggcatc
     1381 tccggaggcg aggagggcgc gctcgaaggt cccagcatca tgccgggcgg cagcgaggag
     1441 tcgtacgact cactcgggcc gatgctggag aagatctccg cgaaggccaa ggacggcgct
     1501 ccgtgcgtca cccatatcgg gccggacggc gcggggcact tcgtgaaaat gacacacaac
     1561 ggcatcgagt acgcggacat gcagctgatc ggcgaggcct accagctcct gcgcgacgtg
     1621 gccggatact cccccgcgca gatcgcggac atcttccgca cctggaacac cgggcgtctg
     1681 gactcctacc tcatcgagat caccgcggaa gtcctctcgc atgtcgacgc gtcgaccggt
     1741 aaacccttcg tggacgtcgt gctcgaccag gcggagcaga agggcaccgg ccgctggacc
     1801 gtgcagatcg ccctcgacct gggcgttccc gtctccgcca tcgccgaggc cgtcttcgcc
     1861 cgctccgtct ccggccacgc cgacctccgc gacgcctcag gccacctcac cggaccgacc
     1921 gcccgcaggc tcggctcgga cgaggcggcg gcattcgcgg accgggtcga gcaggcgttg
     1981 tacgcctcga agctcgtcgc ctacacccag ggcttccacg agatcgcggc cggcagcgag
     2041 cggtacgact ggaacatcga cctcggcaag gtcgcctcga tctggcgcgg cggatgcatc
     2101 atccgggccg cgttcctcga caggatcacc agcgcctacg aagcccggcc gagcctgccc
     2161 agcctcctcg ccgacaagag tttcgcggag gagatcgccg cagcacaggg cgactggcgc
     2221 acggtgatcg ccaccgccgt cacccggggc gtccccaccc cggccttcgc cgccaccctc
     2281 gcctactacg actcgctccg cgccgaacgc cttcccgccg ccctcaccca gggccagcgc
     2341 gactacttcg gcgcacacac ctaccaccgc accgaccgcg agggcacgtt ccacactctc
     2401 tggggcggcg acaggaccga ggtcgagatc agcagctgac gcgccttcga ggccggcgct
     2461 ccggtcaccc cggacgggga agggcgagga tgcccgtgct cccacgcgcc cgcgacctga
     2521 agtctcggcc cgctgccgcc ggctcgcccg catcgcgcgc tcccgcactc ccgcgcgccc
     2581 gcgacctgaa ggttcaccgt gcggtaatcg ggcgcaggta ggcgcggcgt cgacatcctc
     2641 gccctcggtc ggcgcggttg cgctccacgg gcggggggcg aggatggaac gagggggaaa
     2701 tcccgggggg cgaacagcag cggcggcccg cgaagccgcg cgaacagggt gcccgcgcgc
     2761 ccgcgacatg gagccgacat gaccgatctt cctcctccga tcacgcccta cctcgaaccc
     2821 gccgcgaagg agctgtgcga ggccacggac ccgcatccgc ggatctacga ggtccccccg
     2881 gagaagggcc gcgacatcct gctcggcctg cagagcgacc ggagcgtgcc ccgccccgag
     2941 gtcgacgagg agtgggtcga cgtggacgcg ggccagtggg gcacggtccg cacccgcatc
     3001 atccggccca agggggccag cggagagttg ccggtggtgt tctacatcca cggcgccggc
     3061 tgggtcttcg gcgacgaccg gacccacgac cgcctcttcc gcgaactcgc cgtgggcgcc
     3121 ggggcggcgg gcgtcttccc cgtctacgac cgcgcacccg aggcgaagta ccccacccag
     3181 gtcgaacaga actacgccgt gggccagtgg gtgctggaac acggcgcgca gcacgggctg
     3241 gacacctccc ggatcgccgt caccggcgag tcggtcggcg gctgcatgtc cgccgtcttc
     3301 gcgctgatga acaaggagcg cggcggcatc gacctcaagg cacaggtcct gctgtacccg
     3361 gtcaccgacg ccgacttcag caccccctcg tacctccagt tcgccgaggg ctactacctc
     3421 acccgggacg gcatgaagtg gttctgggac gcctacaccg gcaacatcgc ccaccgcacg
     3481 gaggcgtacg cctcaccgct ccgggcctcc ctggaacagc tccgggggct gcccaccacg
     3541 ctggtcatca ccgacgaggc cgacgtcctg cgcgacgagg gcgagaagta cgccaacagg
     3601 ctgcgcgagg cgggcgtgga cgtgacctcc gtccgcgtgg cgggcatggt ccacgacttc
     3661 cttctcctgg acagtctgcg cgacacccgc gccgccaacg tcgcccgcaa gctcgccgtc
     3721 gacgccctgc gaacagccct gcacgacggc tgaggccgac cccgtgtccg gtggaccctg
     3781 accggtccgc gcgctccccc ggccgggcgc gcccggcgtg cggccgggga cgggacgtgg
     3841 gttagtggct gcgagccctg ttcgtcggga tccgtcgacc accggctgct caccgccgtc
     3901 cgtacaccgt cgcaatggag tgcgcgcatg cccgacgccg tccctgatcc cgtctccgtc
     3961 cagcccgtcg tggctccgtt gaccagcgcg gcgctgttcc tggtcggcac gatcgagccg
     4021 ggcggcgaga gcgcggtgcg tggcgtgctg ccggatctcg cggccgtggc ccggtcgctc
     4081 ggcttccggt acccggacgc cggtctcgcc tgtgtggcgg ggttcggctc cagcgcctgg
     4141 gaccggctgt tcggcgggcc gcgcccggct cacctgcacc cgttcccgga gctgcgaggg
     4201 cctcttcacc acgccccggc gacccccggc gacgtgctgc tgcacatccg ggccgagcgg
     4261 atggacatct gttacgcgtg ggcggcccag ctgctcgaca agctcggcgg agccctgcgg
     4321 atcgtggacg agacgcacgg attccgctac ctggaccacc gggacctgct cggcttcgtg
     4381 gacggtacgg agaacccggt gggcgacgac gcccgttcgg cggcgctcgt cggggccggg
     4441 gacgacccgg agttcgaggg cggcagctac gtcgtcgtac agaagtacct gcacgacctg
     4501 acgtcctgga acgcgctctc cgtggaggag caggaacgcg tcatcggccg caccaagtcg
     4561 gacgacgtgg agttccccga cgacgagaag cccgccgact cccacatcgc gctcaacacg
     4621 atcaccgacc cggacggcac cgaacgggac atcctgcggg cgaacatgcc gttcgggagc
     4681 ttcgggaagg gcgagttcgg tacgtacttc atcgggtacg ccgccgatcc cgacgtgacc
     4741 gagcggatgc tccgcaacat gttcctcggc gatccgcccg ggacgcacga ccgcatcctc
     4801 gacttctcca cggcggtcac cggttccctc ttcttcgccc cccgcgccga tttcctcgac
     4861 gctccgccgc ccccgcccgt cctgaccagc tccggggacc ggccggaacc ggtgcccgca
     4921 ccggtccggc aggagcctgt cgcggccggg ttggaccacg gttcgctgcg catcggcagc
     4981 ctgcaagaaa gcgcccagtg atgaacaatc tgcatcgcga actcgctccg gtcaccccgt
     5041 ccgcctggga ggagatcgag gaggaggccc ggcgcacctt ccgccgccat gtcgccggcc
     5101 gtcgtgtcgt ggacgtgtcc gatccggacg gtccgacgct ggccgccgtc ggcgacggcc
     5161 atctgaccga tatcgatccg cccaccccgg acgtggtggc acgggcccgc acatcgatgc
     5221 cggtcatcga gtggcgggtg ccgttcacgg tgacccgcca ggccgtggac gacgtggagc
     5281 gcggctccgc cgacagcgac tggcaacccg tcaaggacgc tgcacgcacc tgtgcgttcg
     5341 ccgaggacat ggcgatcatc gacgggtacg cggcggccgg gatcaccggg ctgcgggacg
     5401 gttcctcgca cgatccgctg gctctgcccg ccgacgcgcg ggactatccg gtggcggtca
     5461 gtcaggccgt gacgcggctg cggctggccg gtgtcgacgg cccgtaccgg ctgctgctcg
     5521 gcgcggacgc gttcaccgag gccgccgaga cctccgacca cggctatccg gtcaagacgc
     5581 acctgagccg cctggtggac gacgagatcc tctgggctcc cgcggtcaag ggcggggtgc
     5641 tcctgtcgac ccggggcggg gacttcgaac tctgcctggg ccaggacctg tcgatcggct
     5701 acgcggacca cgacgccacg agcgtccacc tctacttcca gcaggccttc accttccgga
     5761 tgctgacacc ggaagccgtg gtcggcctga tcgcctgatc cgcgggggcg ctcaccggcg
     5821 cgtggccggg cgaccgagcg cccccgttcc gccgaagacc tgggggcagc catcggggac
     5881 gctggccgta tgacgcagac gacttccgcg caccaccagg gcggcacggc accgccggcg
     5941 gcccgcttcg acgccgtcgt ccgggcggca gtcggccagg ggctgctggg cgagggcgac
     6001 ccggtcgccg gattcatcga cacggacggc gtgcgcgcct ccgtcggcgc cctgcacgac
     6061 gccttcgccg ccacgcccga ggtgctgcac accttcgcgg ccaaggcctg ctccctcgtc
     6121 ccggtgctgc ggctgctcgc cgactgcggc atgggatgcg aggtggcgag ccccggtgaa
     6181 ctgcggctgg ccgtggaggc cgggttcgcc gcgtcccgtg tcgtcctgga ctcccccgcc
     6241 aagacccgcg aggagatccg gctggcgctc gccctcggcg tggccgtcaa cgccgacagc
     6301 ctcgacgaac tgcgccgcat cgacgccctc cgtaccccgg gcaccgcctc cgtcatcgga
     6361 ctgcgggtca acccccaggt gggcggcggc tcgatcgggc cgatgagcac ggcgacggac
     6421 acgtcgaaat tcggggtcgc gctgcgcgac ccgggggccc gcgaggcaat cgtggaggcc
     6481 ttccgggagc ggccctggct gacccggctg cacacccatg tgggctccca gggctgcccg
     6541 ttggagctga tcgcggacgg ggtggcacgg gcgtacgagc tggccgagga gatcaacgcc
     6601 acgctgggcg tccggcgggt caccagcctg gacatcggcg gcggcctccc ggtcaacttc
     6661 gccgacgagt cggtgcgccc cggttacgcg gactacgtgg cggccctgac ggcggcggcc
     6721 cccggcctct tctccggccg ctactccctg gtcaccgagt tcggccggtc gctgctggcg
     6781 aagaacgggt tcatcggcgc ccgggtggag tacacgaagg atgccggggg ccgccggatc
     6841 gcgctcaccc acgcgggggc ccagaccgcc acccgtaccg tcctcatgcc cgacgcctgg
     6901 cccctgcgga tcggggcgtt cggcccggac ggcattccca ggaccgggcc gtcgctggcg
     6961 caggacatcg cggggccgtg ctgcttcgcg ggcgacgtgg tggcgtacgg cagggagctg
     7021 ccggagctgg ccgagggcga tctggtcgtg ctgtacgaca cgggggcgta ctacttctcc
     7081 accccgtggg cgtacaacag cctgccccgt cccgcggtgt acggcttccg cgcggtggcg
     7141 gggccggagg acgggtcggg ccacgacgga ccggtgacgt tcacgacggt gcgggacgct
     7201 cagtcgctgg acgacatcgc ggcggagagc ggcctcgcgc acgcggacgc gctgatccgg
     7261 cgggacgact gaacgggcgg gccgggccct ggcaccgggc ggaaccggac ccggccgccg
     7321 gtcacgtccc ggcgggctgc ggctcgggct ccggatcggg tacggggtcg ggacccggcg
     7381 cgggcggctg cgggatcggg tccggcgcgg gacccggcgg caccggcccc ggccccggcg
     7441 gcacgggcgg cgggctgggc accggagtgg gcgggcccgg cacgggcgga ggcgtcggcg
     7501 agggcggtac ggggtcgggg tacgggtggg tcatcacgat cctccacaac ggcgcgaaac
     7561 ggatagatgg agcttttctc caccgtctcc cggagcggct gcccgcgcct gccgagtcca
     7621 cacctgcacc gggcgacagc cgcgcgggcc gacgtgtgcc gggccgcccg gagtgggcag
     7681 gactacggcg atccgcccgg acctgaccgg ccccgtccgg ccccgtccgg acccgaccgg
     7741 acccgtccgg atccgcggac cgcgccgtcg acgcgaaagc cgatgcgaaa ggtgtccccc
     7801 atgcccctgc cgagcgttct cgccgttcac gcccatccgg acgacgagtc cctgttctgc
     7861 ggaggcgtcc tcgcgcggca cgcggccgcc ggcggccgca ccgcggtggt cacggcgacc
     7921 tgggcggagg gcacgcacag ggccgtcgaa ctcgcgggcg ccctcgacgc cctgggcgcg
     7981 ggcgccccac gactgctcgg ctacgcggac gcccgtgtgc cggattcggc ccctggccgg
     8041 ccccggttcc tcgacgcgcc gctggacgag gccgtggagg ccgtcgtcgg ccacatccgt
     8101 gacgtccgcc cggagatcgt gatcacggtg gacgcctacg gaggcatgac cggccacccc
     8161 gaccacgtac acgcccaccg gatcaccgcg ctggcggtgc gggccgccgg cctcccccgc
     8221 taccgccccg cgtccggcga tccctggcag ccgagcgccc tctacctggc cacgcacccc
     8281 cgctcggcgg ccgtatcggt gggcgggcga ctggcccggc cgggaacccc ggccgacgcg
     8341 ctgtactgct cggaggacgc gtggatcacc gcgaccgtcg acgtgggccc ctggtttccg
     8401 cagaaactgg ccgcgatcct ggcacaccgc agcgaggtgg aacgaggagc ggcacccggc
     8461 cggatcgccg tcctgcctcg ggcagttcag ctcgaggtgc tgggcacgga gtggtacatc
     8521 cgccacgacc tcgcaccccg gggcgggtcg gccacggaac tgagcgcgtg agatggcccg
     8581 ggccctgccc cggggggggg ggggggggcg gcggccgggc cggtagccct acgtgggcag
     8641 cggccgcgat cccgcaacgg acgcgacggc gactactcgc cccctgcccc tgccgtcaga
     8701 acagctcggg gtggttcctg agctggcgct ccgcctcccg gacgagctcg gccggtgctt
     8761 cgggcgcctc gtcggggtga cgctgcgccc acttcgccgc gtacgggcac agcggcacga
     8821 cggggactcc ctcgcgggcc gccatggtgt agaactcccg gacgagcgcc cccgcgatgc
     8881 ccttgccctc gtgttcgggc ctgacgacgg tgtgcacggc caccagcgcc ccgggggccg
     8941 ggtccatgac gaagtacgcg atggtcccgg ccgactcgcc gtcctcgtac gcaacgagac
     9001 tgccgtgctc ccggtcgtcc ctgatctcta ccgctgcctt ctcggccatg gcccctgcct
     9061 tctcgttcgt ggacgtggac gtcatgcgtc cggaaacacg gacggcgacc tcagccgccc
     9121 gcgacgacgg cctgcgggct ccgctccgta cgggtcccgg ggaccggcgc cgaggcgtcg
     9181 gagccgagcg ccacgatccg gttgtccgcg tccacgtgca cgacccgcgg cacgagggca
     9241 cgggcctccg cgtcgtcgac ctgtgcgtag ctgatcagga tgaccaggtc accggggtgc
     9301 acgaggtgtg cggccgcgcc gttgatcccg atgacaccgg agccgcgctc gccctcgatg
     9361 acgtatgtct ccaggcgggc gccgttgtcg atgtcaacga tatggaccag ctcgccgggc
     9421 agcaggtcgg cggcctccat cagggcggcg tcgatcgtca cggaaccgac gtagtgcagg
     9481 tcggcctggg tgacggtggc ccggtggatc ttggacttga atattgtacg tatcatcgag
     9541 gcactcctgg acgtaggctc cctgcctgcg tatttgcagg tcaagggctg tttcctcacc
     9601 ctacaacgag tcgcatgttc aggcagcgtt ccccaggttt ttcaggactc atgctgacca
     9661 cttgctgact ttcctgacgc ttcatcaggt gacgtcgaga gggcccactc actctcccga
     9721 actccaccgg aacgactccg caagcctacg cagctctccc ctgcgggtat cagtgatcac
     9781 catcacctcg acgagccgac agtggttgcc tgcctccacg cccctataag aactcctagc
     9841 aaaatgagat gagggatgtc ctcgggtcgg gcgaggatcg ctcggtactc gccgacgaaa
     9901 gggcagctat gggcaacagg cccccgaggg agctcatctc actcgctgat gacgagggca
     9961 acagcgtctc ggtcaacgtt ctggggcgta gtcccggatg gaccgccggg ctcgacgcgg
    10021 agattgtcgt caagactcct ttcgtgtccg gtcggattga cctggcgttg tacgttgcga
    10081 ggctggagag ctgggccgac gcactggacc ggctcgacgc cggggaagac gtggcctgga
    10141 tggagatgaa cagcgggccg tccatcttca tccagctcac gggcgagcgt gactgcccgg
    10201 aggtggtcgt ggaggacgag tccgggtcca tggtcaccgt gcgggtcccg ctcgtcccgc
    10261 cggacgactg ggttgctgac catcgccggc gtctgcgcca ggtgatggat cactgggttc
    10321 cgatgctgtc agggtagggc gacaagtcgc cgccagcaga tgagcacgca tcctagggtt
    10381 ggtaaggctt cgtggatgtc gtcgcggatc tcccagcgga tgcgcatgcg gcggaagcag
    10441 tgcaggtggg cgaaggcgtg ctctacgacc cagcgttggg cgcccaggcc ggatccgtgc
    10501 tcggtgccgc ggcgggctat cactggcttc acgccgagtg cccagacaag gcggcggtac
    10561 ttgtcgtggt cgtagccgcg gtcaccgagc acgaggtccg ggcggcgcct gggccggccg
    10621 cgcttgccgc gcaccggtgg aacggctttg aggagcggga tcagctgggt gacgtcgttg
    10681 cggttgccgc cggtcggggt ggtggcgagc ggggtgccgg tggcgtcggt gatcaggtgg
    10741 tgcttgctgc cagttctgcc ccggtcaaca ggacttcgtc ccgccttggg cccccttt
//
