LOCUS       NZ_KZ195572            11368 bp    DNA     linear   CON 06-SEP-2024
DEFINITION  Streptomyces sp. CS057 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ195572
VERSION     NZ_KZ195572.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS057
  ORGANISM  Streptomyces sp. CS057
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8333859)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 265.32x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188345.1-RS_2024_09_06
            Annotation Date                   :: 09/06/2024 02:40:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,152
            CDSs (total)                      :: 7,074
            Genes (coding)                    :: 6,991
            CDSs (with protein)               :: 6,991
            Genes (RNA)                       :: 78
            rRNAs                             :: 10, 6 (16S, 23S)
            complete rRNAs                    :: 10, 6 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 23 of 83
            Pseudo Genes (incomplete)         :: 71 of 83
            Pseudo Genes (internal stop)      :: 4 of 83
            Pseudo Genes (multiple problems)  :: 14 of 83
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:25:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 422756
            Orig. end                         :: 434124
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195572.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
FEATURES             Location/Qualifiers
     region          1..11368
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11368
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11368
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6368](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6368)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1706..3445)
                     /locus_tag="B9W61_RS01585"
                     /old_locus_tag="B9W61_01595"
     CDS             complement(1706..3445)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955801.1"
                     /locus_tag="B9W61_RS01585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_01595"
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="WP_087763903.1"
                     /transl_table=11
                     /translation="MMLHPELLRAANSARRPLGLATALLAAVTVTHLAQAVLLALVLAR
                     IARGETDSLAPLLTAVIAVVLVRAGLGRAQRLTAVTAGATVRIGLRDTLLERLGALGPT
                     AITGARAGAVRATLVDGVEGVDAYVSRYLPQALITCAVPPLLLVAVALVEPYAALALGP
                     AVLLALLGPRWWDRLLARRGKEHWDSYEALAADYLEALQGMPTLRATGAVGRVRERLER
                     RSAALHRATVAKLRVSLVDTGLTDLAVQGGTAAAVLVACSSAATGRTAATGTYLLLLLA
                     SECFRPVRDLSREWHAGYLGVSAADGIAGLRTAEAAVPDRGTVTAAWEGAPEIRFEDVH
                     FTHPGSGRPALDGVSFTAAAGRTTAVVGPSGAGKSTLLSLLLRQRDPDRGSLTVAGTAT
                     AAYTLASLRRGIAVVSQDTYLFHATIAENLRIARPAATDEQLRAAARTAGIDQEIARLP
                     DGYATLVGERGATLSGGQRQRLALARALLADAPVLILDEATSAVDERGQARIVRELSAA
                     GRGRTCVVVAHRLDAVRHADHIVVLDAGRVAATGDHTTLLAGGGVYASLVAAGSTAQEE
                     RAA"
     gene            complement(3442..4986)
                     /locus_tag="B9W61_RS01590"
                     /old_locus_tag="B9W61_01600"
     CDS             complement(3442..4986)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964398.1"
                     /locus_tag="B9W61_RS01590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_01600"
                     /product="nitroreductase"
                     /protein_id="WP_087763904.1"
                     /transl_table=11
                     /translation="MPDTPARTAAPESGTRPVDALLRRALADVRSSRAPGLAPYVPEHP
                     FPWSGAGLPLAGDEARPGAGPGAGGRAAIDLDRVLRLSLAVPSGTPGRLRPVPSAGALH
                     PVRAHLLTGPGCSLPPGRYAYDPRTHRALPRGPAPDGIPPGALVVLTVTASRTVAHYGH
                     RAWPLLLLDTGHAAAALALAGPRAADALVCLDADAALLSAAAGLPATPDRPDAWPGSDP
                     ELPLAVVGLTPVGVGVGVTAGLPATLCAWAGVPPHAGTPSPRPGAETALPRELVATRHL
                     LHHIAASPELPGGTWHPATRPGPVTDKTLQARRSAPPDDLNHPPGEDRLNRLLATAGTA
                     RPDGPAWTAAVGGGAPALYAKAPGSRGRLDLRASGDARPTLAHWAAGQQWIGGAGAVFL
                     AHGCPGDAPPAVVRTCHLAAGYAAGAVQAHATALGLRSRPIGSWQQADLGAALGEAPGR
                     DWIVHGLAVGAPPPPGRVAPGFVRHPPTAETPPAPTPPVTSTPAAPATEPGEEERP"
     gene            complement(5001..6368)
                     /locus_tag="B9W61_RS01595"
                     /old_locus_tag="B9W61_01605"
     CDS             complement(5001..6368)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059075.1"
                     /locus_tag="B9W61_RS01595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_01605"
                     /product="YcaO-like family protein"
                     /protein_id="WP_087767686.1"
                     /sec_met_domain="YcaO (E-value: 7.1e-44, bitscore: 149.5,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSALPIEALVDPVSGIIRDVAPVEHPAGAPPRYTAMTADVADAR
                     RLGAWPADRVSLGTTFGDPRGAWVAAVAEAVERYCGNRLPPPGHPQEPRRATAAELAAE
                     GHRLYGPGALPTYAPWQYVRQGFPYAELTPDTPALWTRSTEGGQPCWAPVALTHLNWRQ
                     GELRSLPRTHHLNYAGIATGQGLDDAVERGLLEVVERDALELWWHLDGPTRGIDPASVP
                     GLTDDLAGCGLDVHIVEMPSEFAPCVAALAHDPVRGIHAAGFACRYDPAEAARKAVLEA
                     VHTWVFTQGAVDADGWVYRAVEAGMLARGLYLDHRADRRYLDDCGTGFAAVRDLGAHVQ
                     VWLDDRMAPLARRFTEPALGTVPVTAIDPGSRTALDTALAAGGHRVITADLTTEDVAES
                     TLRVARVLVSGLIPNAPAAFGYFGCPRFADAALARGWRTRPPSAPGDFTLAPPPHM"
     gene            complement(6365..8122)
                     /locus_tag="B9W61_RS01600"
                     /old_locus_tag="B9W61_01610"
     CDS             complement(6365..8122)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010639446.1"
                     /locus_tag="B9W61_RS01600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_01610"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_087763905.1"
                     /transl_table=11
                     /translation="MNPRTLYVPTPDGTALATDVCLPDGTGPLPAVLIRTPYGREAHRA
                     ELRGWAAHGFAALAQDVRGRHGSPGEWHPYRDHEETDGAATVAWVRAQAWSNGEVVAVG
                     ASYAAYCALVTVLAAPQAACDTGPDTDPGDHHRDGAPDVRHGEGAPDARHRDGVPGAFL
                     RDGVPDAVIAAVPALGLTETAREPGGPERLWARAGWWAAHGDRRDSDPDALRRALADDP
                     RLLEHLPVARLADRLAASLGRELPSWSGLWADRTRGRLVALGSSARLPLLAVGGTRDPF
                     AEDTVALWRGWGGPSRLLLGPWGHRLTADPAAPARTRVNLGALYVRWARAALAGRLEPT
                     RHGVIALGDSGRWHGTRPRRTPNPASTQAAAPQDAAPQDATPQEGAERRAGADRPEAES
                     RWSFDAPTGLHLLHGAEFNADPDRPVRSDDLTVPADGEHADRCLLLSPPLPRPLDLAGA
                     AYARINVTADTPCADWAVRLTALDPNGRADPLAFGIVRRTGPPGEAADISVPLGTLGRR
                     LPAGTRLRAEIAGHHFPAHARNPHTGEDPVTATRLAPSRRTVDPRGSALHLPVVARRRY
                     VEPAPEICR"
     gene            complement(8119..9126)
                     /locus_tag="B9W61_RS01605"
                     /old_locus_tag="B9W61_01615"
     CDS             complement(8119..9126)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452727.1"
                     /locus_tag="B9W61_RS01605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_01615"
                     /product="hypothetical protein"
                     /protein_id="WP_087763906.1"
                     /transl_table=11
                     /translation="MTRSRLAPGTGIVTPPGGAPVLRTSGGEFLRIDTGGVTGEDLVRR
                     LAGEGATEEAVPGEETPVGGAHAAELARLVEAFEAAGHALSAPPPAPLAGRTVHLLGDP
                     VLTAPVARCARAEGAEVHRITPDGVAELARSAPDDGAERRVAVVWCLDSPVPEGLWDTA
                     DRLPARRIAWLRCHREGAHSWIEPLAAAPGDVTSRHIRLRRLAATPAHRELAAYWAGHR
                     TPDTGPHPTEGSAALIAALLTADLIAWAEAEAEAEAEAEAEAEAEAEAGEGAGVGAGAS
                     RAAVLPVRRRLRRIDLRNLTHTEHPVLPVPDVAPLPVPAAVRTRAGAGTAEARP"
     gene            complement(9256..9390)
                     /gene="amiA"
                     /locus_tag="B9W61_RS36630"
     CDS             complement(9256..9390)
                     /codon_start=1
                     /gene="amiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508296.1"
                     /locus_tag="B9W61_RS36630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="streptamidine family RiPP"
                     /protein_id="WP_007452728.1"
                     /transl_table=11
                     /translation="MENNKVFSPIADQGQLAHLSATHSNALVENPFDDAVEADTAAEK"
     misc_feature    9380..9397
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 13.87"
                     /tool="antismash"
     misc_feature    9464..9477
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.45"
                     /tool="antismash"
     misc_feature    complement(9464..9479)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.88"
                     /tool="antismash"
     gene            complement(9465..10370)
                     /locus_tag="B9W61_RS01610"
                     /old_locus_tag="B9W61_01620"
     CDS             complement(9465..10370)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955796.1"
                     /locus_tag="B9W61_RS01610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W61_01620"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_087763907.1"
                     /transl_table=11
                     /translation="MAEGLTGQVESSPTEAAGPDAEPSGRPGPEVVRAEGLAFGYPGRM
                     VLRGVDLSVRAGELVALIGLNGCGKSTFLKLAAGLLTPSGGRVLLEGDDVTRLSRRTAA
                     RRVALLHQSAPTVPGLTVRQLVRQGRYAARGPLGMLREGDDEVTSRALADVGVTAWADR
                     PVDALSGGERQRVRLAMALAQDARVLFLDEPTTYLDLRHQLEVLQTVVRLRAERRLTVV
                     MVLHDLNHAARFADRIVALREGRVAADGPPAEVVTRRLLAEVLGVEGRVGIDSEGGWPV
                     CYPDHPLDPLQLLRNENHIH"
     gene            complement(10355..10483)
                     /locus_tag="B9W61_RS36635"
     CDS             complement(10355..10483)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B9W61_RS36635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_256967541.1"
                     /transl_table=11
                     /translation="MTESGTWPGRADIGDIGDIGDAVDFVDGSGGERMRRAAWRKA"
     misc_feature    10897..10906
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
ORIGIN
        1 cggtcggccc tgcggaccgt ggccgggcgg tgggcgatca ccagggtcgt ccgggtggtg
       61 gagtcggccg cgagcgcacg ggacagttcc gcgtcgcccc ggtggtccag atgggcggtc
      121 gtctcgtcca ggacgagtac gcgcggcccg ctcagcagcc cccgggccag ggccacccgg
      181 gcgcgctggc cgccggacag ggtggcgccg cgctcgccga cggcggtgtc cggaccgtgg
      241 ggcagggcgt cggcgatggg ggcgatgccg cagatccggg tcaccgcgtc cagttcgtcc
      301 gccgtggcgt ccggagcgcc caggcggagg ttctcggcga gggtgccgtg gaagagcggg
      361 gcctcctgcc ccaccacgga cacggcggcg cgcaggtcct cctcggtgag gtcccgcagg
      421 tccacggggt gggccggggc ggggggcacg agctccacgg tgccggcctc ggggtcccag
      481 taccgcgcca ggaggtgggc gcaggtcgac ttgcccgccc cggacacacc gacgagggcc
      541 acggcctcgc ccggccggac ggtcaggtcg aggccgtcca ggaccggctc gcccccgtag
      601 ccgaaccgaa ctccgcgcag ccgcaggccc agcgggcccg ccggtacggg gcggggggcg
      661 gtggcagcgg gcgctccggc gggggcgtgc agggcggtcc ggacccgtgc cgcggcggcg
      721 cgcaggctgc ccgcccggcc gagtgcggcc gccgattcgg cgaccggggc cagggccccc
      781 agcgccaggg ccatggcggc cggggcccac gccccgtcga gacgtccggc ggacgcggcg
      841 tgggcggcgg ccgccaccac cccgatgacg gcggcgacga ccagcaggtc gcgggtcgcg
      901 cccgcaccgg tctcccagga ctgttcggcg cgctgggcgc gggcgagccc gcgcccggcg
      961 gacctcagtc gggcccgcct gcggttcagc gcccccgcca tcaggagttc gcgcagcccg
     1021 tcgacgctct ccacggtctc ggacgagacc tcggcgagcg cgctccgggt ccgcgccccg
     1081 cgttcggcgc gcccgcgcgc ttcgaacagc ggcgaccaca tcaggaggag cgcggcgggc
     1141 acgacggcga gcagcagcca ggggccgagg gccgccagca ctgcagccga caccgcgaag
     1201 accattccgg aggcgaggag ttgggcgatg gcgtgggcgt agaagaactc cagtgcctcg
     1261 acgtccccga gcgcggtggc cgcgagatcg ccgctgcgcc ggcccgcgat ccgggcgggg
     1321 gcgctgcggg ccagcccgtc gaagacccgc acgcgcagtt cggcgaggac ccggtaggcg
     1381 aggtcgtgcg agaggtccat ctcccgccag gtggccagcg cccggagcag gacgagcccg
     1441 atcaggacgg cgacggtcgc ggtggtgggc gggcggtgct ccgtgacggc tgtgcccacc
     1501 gtgtacgcgg cgagggtcac gagggcgacc agggccgcct ggtcgacgag ggccgccagg
     1561 caggtgcgca gcacggtggc gcggtgcgcg ccgaggaccg ggaggagcga gcgcagggcg
     1621 ccggtcccgg cctcgtcggg ggctgcgggg gctgcgggga cgggggaacg gacggggtcg
     1681 gccggggccg ggggcggggg cggggtcatg cggcgcgctc ctcctgtgcg gtgctcccgg
     1741 ccgcgacgag cgaggcgtac acgccgccgc ccgcgagcag ggtggtgtgg tcgcccgtcg
     1801 cggcgacacg gcccgcgtcg aggacgacga tgtggtcggc gtggcgcacg gcgtcgagcc
     1861 ggtgggcgac gacgacgcag gtgcgtccgc ggccggccgc ggacagttcc cgcacgatgc
     1921 gggcctggcc acgttcgtcg accgcgctgg tggcctcgtc caggatcagg accggggcgt
     1981 cggcgagcaa cgcccgtgcg agggcgagcc gttgacgctg tccaccggag agggtggcgc
     2041 cgcgttcgcc gacgagggtg gcgtaaccgt ccggcagccg ggcgatctcc tggtcgatcc
     2101 ccgcggtgcg ggcggcggcg cgcagttgtt cgtcggtggc ggccgggcgg gcgatccgca
     2161 ggttctccgc gatcgtggcg tggaagaggt aggtgtcctg cgagaccacg gcgatgcccc
     2221 ggcgcagtga ggcgagggtg tacgcggcgg tggccgtccc ggcgacggtg aggctgcccc
     2281 ggtccgggtc ccgctgccgc agcagcaggc tcagcagggt cgacttcccg gccccggacg
     2341 ggccgacgac cgccgtggtc cgcccggcgg cggcggtgaa gctgacgccg tcgagggcgg
     2401 gccgcccgct ccccgggtgg gtgaagtgca cgtcctcgaa acggatttcg ggtgcgccct
     2461 cccaggcggc ggtcacggtt ccccggtcgg gcacggccgc ttcggcggtg cgcaggccgg
     2521 ctatcccgtc ggccgcggac accccgaggt agcccgcgtg ccactcgcgg gagaggtcgc
     2581 ggacggggcg gaagcattcg gaggcgagga gcagcaggag gtaggtgccg gtggcggcgg
     2641 tacggccggt ggccgccgac gagcacgcca cgagaacggc ggcggccgtg ccgccctgga
     2701 cggccaggtc ggtgaggccc gtgtcgacga gcgagacccg gagcttggcg acggtggccc
     2761 ggtgcagggc ggccgaccgc cgctccagcc gttcccgtac gcggcccacc gccccggtgg
     2821 cgcgcagggt cggcatcccc tggagtgcct ccagatagtc ggcggcgagg gcttcgtagg
     2881 agtcccagtg ctccttgccg cgccgcgcga gcaggcggtc ccaccagcgc ggcccgagga
     2941 gggcgagcag cacggcgggc ccgagggcga gggcggcata gggctcgacg agggccacgg
     3001 cgacgagcag cagcggagga acggcgcagg tgatgagcgc ttggggcaga tagcgcgaga
     3061 cgtacgcgtc gacgccctcg acgccgtcca ccagcgtggc gcgcacggct ccggcacgtg
     3121 caccggtgat cgcggtgggg cctagggcgc cgagccgctc cagcagggtg tcgcgaagcc
     3181 cgatccgtac ggtggctccg gccgtgacgg cggtcaggcg ctgggcgcgc ccgaggccgg
     3241 cacggaccag gacgacggcg atgacggcgg tcagcagcgg ggcgaggctg tccgtctcgc
     3301 cccgggcgat ccgggcgagc acaagggcga gcaggactgc ctgggcgagg tgggtgacgg
     3361 tgaccgcggc gagcaacgcg gtggcgaggc cgagggggcg gcgcgcgctg ttcgcggcgc
     3421 gcaggagttc ggggtggagc atcatgggcg ttcttcctcg ccgggctcag tcgccggggc
     3481 ggcgggtgtc gacgtgacag gcggtgtcgg ggcgggtggc gtttcggccg tcggtgggtg
     3541 tcggacgaag cccggggcga cacggcctgg tggtgggggc gcccccaccg ccaggccgtg
     3601 cacgatccag tcgcgtccgg gggcctcgcc gagcgccgcg cccaggtccg cctgctgcca
     3661 ggagccgatg ggccgggagc gcagacccag tgcggtggca tgggcctgga ccgctccggc
     3721 cgcgtacccg gcggcgaggt ggcaggtgcg cacaacggcc ggcggggcgt ccccggggca
     3781 gccgtgggcc aggaacaccg cgcccgcgcc gccgatccac tgctggcccg cggcccagtg
     3841 ggcgagggtg ggacgtgcgt ccccggaggc gcggaggtcg aggcgtccac ggctgccggg
     3901 agccttggcg tacagggccg gggcaccacc gccgaccgcc gcggtccagg cgggtccgtc
     3961 cggccgcgct gtcccggctg tcgccaggag ccggttgagc cggtcctcgc cgggcgggtg
     4021 gttcaggtcg tcgggcgggg cactccggcg cgcctgcagg gtcttgtcgg tcaccggtcc
     4081 cggacgggtg gccgggtgcc aggtgccgcc gggcagctcc ggggaggctg cgatgtggtg
     4141 caacaggtgc cgcgtcgcga cgagttcgcg gggcagggcg gtctccgcgc cgggccgcgg
     4201 cgacggggtt ccggcatggg gtgggacacc ggcccatgca cacagtgtcg cggggaggcc
     4261 ggcggtcaca cccacgccca cgcccacggg tgtcaacccg accaccgcga gcggcagttc
     4321 cgggtcgctg ccgggccagg catccggccg gtccggagtg gcgggcagtc cggcggcggc
     4381 ggagagcagg gcggcgtccg cgtcgaggca gaccagtgcg tcggccgccc ggggcccggc
     4441 cagcgccagg gccgcggcgg cgtggccggt gtccagcagc aggagcggcc aggcccggtg
     4501 gccgtagtgc gcgacggtgc gggacgcggt cacggtgagg accacgaggg cgcccggggg
     4561 tatgccgtcg ggggccgggc cgcgcgggag ggcacggtgg gtgcgcgggt cgtaggcgta
     4621 acgtccgggc ggcagcgaac acccgggccc ggtcaacagg tgtgcgcgca ccgggtgcag
     4681 tgcccctgcc gaggggacgg gccgcagcct gcccggtgtg ccggagggca cggccagcga
     4741 gagccgtagg acgcggtcca ggtcgatcgc ggcccgaccc ccggcccctg gacccgctcc
     4801 gggccgggcc tcgtccccgg ccaacggcag ccccgccccg ctccagggga acgggtgctc
     4861 gggaacgtac ggggcgaggc ccggcgcacg cgaggagcgg acgtccgcga gggcccggcg
     4921 cagcagggcg tccacggggc gcgtcccgga ttcgggtgcg gcggtccggg ccggggtatc
     4981 gggcatggcg gtgcgctcct tcacatgtgc gggggcgggg cgagggtgaa gtcccccggg
     5041 gcggagggcg gtcgggttcg ccagccccgg gcgagcgcgg cgtccgcgaa tcgagggcag
     5101 ccgaagtagc cgaaggccgc aggcgcgttg gggatcagtc cggataccag gacgcgggcg
     5161 acccgcagcg tcgactcggc gacgtcctcc gtcgtgaggt cggcggtgat cacccggtgt
     5221 cccccggcgg cgagcgccgt gtcgagcgcg gtgcggctgc cggggtcgat cgcggtgacc
     5281 gggaccgtgc cgagggcggg ttcggtgaag cgtcgggcga gcggggccat ccggtcgtcc
     5341 agccagacct ggacgtgggc cccgaggtcc cggacggcgg cgaacccggt tccgcagtcg
     5401 tcgagatagc gccggtcggc ccgatggtcg agatagaggc cccgggcgag catcccggcc
     5461 tcgaccgcgc ggtacaccca gccgtcggcg tccaccgcgc cctgggtgaa cacccaggtg
     5521 tgcacggcct ccagcacggc cttgcgggcc gcctccgccg ggtcgtagcg gcaggcgaac
     5581 ccggcagcgt ggatgccgcg tacgggatcg tgcgcgaggg ccgccacgca gggggcgaac
     5641 tcggagggca tctccacgat gtgcacgtcc agcccgcagc cggccaggtc atcggtgagt
     5701 ccggggacgc tcgccgggtc gatgccccgg gtgggcccgt ccaggtgcca ccacagctcc
     5761 agggcgtcgc gctcgacgac ttcgaggagg ccgcgctcga cggcatcgtc gaggccctgc
     5821 ccggtggcga tgcccgcgta gttgaggtgg tgggtgcggg gcagcgaacg cagttcgccc
     5881 tggcgccagt tgagatgggt cagcgcgacg ggggcccagc agggctgccc gccctcggtg
     5941 gaccgggtcc acaacgccgg tgtgtctggg gtgagttcgg cgtacgggaa gccctggcgg
     6001 acgtactgcc agggggcgta cgtcggcaga gcgccgggcc cgtagaggcg gtggccctcg
     6061 gcggccaact cggctgcggt ggcccgacgg ggctcctggg ggtggccggg cggtgggagg
     6121 cggttgccgc agtagcgctc gacggcttcg gccaccgcgg cgacccacgc gccccggggg
     6181 tcgccgaacg tggtgccgag cgagacgcgg tcggcgggcc atgcgccgag gcggcgggcg
     6241 tcggcgacgt cggcggtcat cgcggtgtag cggggcggcg cgccggcggg gtgctcgacg
     6301 ggcgcgacgt cgcggatgat cccgctgacg gggtcgacga gtgcttctat gggcagggcg
     6361 ctggtcaacg gcatatctcc ggtgcgggct cgacgtagcg gcgtcgggcg acgacgggca
     6421 ggtggagggc gctgccccgg gggtccacgg tgcggcggga cggggcgagc cgggtggcgg
     6481 tgacggggtc ctcgccggtg tgcgggttgc gggcgtgggc tgggaagtgg tgtccggcga
     6541 tctcggcgcg caatcgggtc ccggcgggca gccgacggcc cagcgtgccg agcggcacgc
     6601 tgatgtccgc ggcctcgccg ggcgggccgg tgcgtcggac gatgccgaag gcgagtggat
     6661 ccgcgcggcc gttcgggtcg agtgcggtta ggcgtacggc ccagtcggca cagggtgtgt
     6721 cggccgtgac gttgatccgg gcgtaggcgg ctcccgccag gtcgagcggg cggggcagcg
     6781 gcggcgagag cagcagacag cggtcggcat gctcgccgtc ggccgggacg gtgaggtcgt
     6841 cggagcgcac gggacggtcc gggtcggcgt tgaactcggc tccgtggagg agatgtagac
     6901 cggtgggcgc gtcgaaggac cagcggctct ccgcctccgg ccggtccgcc cccgcccgcc
     6961 gctccgcccc ctcctgcggg gtggcgtcct gcggggcggc gtcctgcggg gcggcggcct
     7021 gcgtgctcgc ggggttgggg gtcctccgcg gccgggtgcc gtgccagcga ccgctgtcgc
     7081 cgagggcgat gaccccgtgc cgtgtcggct ccagccgtcc ggcgagcgcc gcgcgggccc
     7141 accgtacgta cagcgcaccg aggttgacgc gggtgcgcgc cggggcggcg gggtcggcgg
     7201 tgaggcggtg cccccagggc ccgagcagca gacgggacgg tccgccccag ccgcgccaca
     7261 gggccacggt gtcctcggcg aacgggtcac gggtgccgcc gacggcgagc agcggaaggc
     7321 gggcggagga gcccagcgcg acgagccggc ctcgtgtgcg gtcggcccag agcccggacc
     7381 aggacggcag ttcgcgcccc aggctcgcgg ccagccggtc cgcgaggcgg gctaccggga
     7441 gatgttcgag cagccggggg tcgtccgcca gggcgcggcg cagcgcgtcc gggtcggagt
     7501 cgcgacggtc gccgtgcgcc gcccaccagc cggcccgggc ccacaaccgc tccggccccc
     7561 cgggctcgcg cgccgtctcg gtgagcccga gcgcgggcac ggcggcgatg acggcgtcgg
     7621 ggactccgtc gcggaggaat gcgccgggca ctccgtcgcg gtgacgggca tcgggcgccc
     7681 cctcgccgtg acggacgtcc ggcgctccgt cgcggtggtg gtcaccgggg tcggtgtcgg
     7741 ggcccgtatc gcaggcggct tgaggggcgg ccagtacggt gacgagggcg cagtacgccg
     7801 cgtaggaagc gccgacggcc acgacctccc cgttgctcca cgcctgtgcg cgcacccagg
     7861 cgacggtggc cgccccgtcc gtttcctcgt ggtcccggta ggggtgccac tcccccggtg
     7921 agccgtggcg gcctcgtacg tcctgggcga gtgcggcgaa tccgtgcgcc gcccagccgc
     7981 gcagctcggc acggtgggcc tcgcgcccgt agggagttct gatcaggacg gcgggaaggg
     8041 ggccggtccc gtccgggagg catacgtcgg tggcgagcgc ggtgccgtcc ggcgtgggga
     8101 cgtacagggt acgggggttc acgggcgcgc ctcggcggtc ccggccccgg cacgcgtccg
     8161 caccgccgcc gggacgggca ggggcgcgac gtccgggaca gggagcacgg ggtgctcggt
     8221 gtgggtgaga ttgcgcaggt cgatccggcg gagccgacgg cgcaccggaa gaacggccgc
     8281 ccgactcgcc ccggctccca ctcccgcccc ctcaccggct tcggcttcgg cttcggcttc
     8341 ggcttcggct tcggcttcgg cttcggcttc ggcttcggcc caggcgatca gatcggcggt
     8401 gaggagcgcg gcgatcaggg cggccgagcc ttcggtgggg tggggaccgg tgtcgggggt
     8461 ccggtgtccg gcccagtacg cggcgagttc gcgatgggcg ggggtggccg cgagtctgcg
     8521 gagacgaatg tgacgggagg tgacgtcgcc cggggccgcg gcgagaggtt cgatccagct
     8581 gtgggcgccc tcgcggtgac agcgcagcca ggcgatgcgc cgcgcgggca gccggtccgc
     8641 cgtgtcccag agcccctcgg gcacgggcga gtcgaggcac cacaccacgg cgacgcgccg
     8701 ttcggcgccg tcgtccgggg ccgaacgggc cagctccgcc acaccgtccg gggtgatccg
     8761 atggacctcg gccccctccg cccgggcgca gcgcgccacc ggcgcggtga gcacggggtc
     8821 gccgaggagg tggacggtgc ggccggcgag cggggcaggc ggcggggcgg acagtgcgtg
     8881 tccggcggct tcgaacgcct cgacgagccg cgcgagttcg gccgcgtgcg cgcccccgac
     8941 cggcgtctcc tcccccggca ccgcctcctc cgtcgccccc tctcccgcca gccggcgtac
     9001 caggtcctcg ccggtgacac cgccggtgtc gatgcgcagg aactcccctc cggaggtccg
     9061 gagcacgggg gcgccaccgg gaggggtgac gatcccggtg ccgggagcga gtcgactgcg
     9121 ggtcacggaa tcctccggat cggaaagcgg gtacggaacg ttcgggggct gaacggggcg
     9181 ggcccgcccg ggatgtcacc gggcgggccc gcccccgcgc tgtgcgggga acctgccgtc
     9241 agcggcagat gtcgcttact tctcggccgc ggtgtcggcc tcgaccgcgt cgtcgaaggg
     9301 gttctcgacg agggcgttcg agtgggtggc ggagaggtgg gcgagctgac cctggtcggc
     9361 gatcgggctg aacaccttgt tgttctccat gagttgacgt ctccattcgg tcggatcgga
     9421 tgggtgtttc ggtcgcggcg ttggtgcgtg ccgcagcaca gacactaatg aatatgattt
     9481 tcattccgca atagctggag cgggtcgaga gggtgatctg ggtaacagac gggccagccg
     9541 ccctcgctgt cgatccccac ccggccctcc acgcccagca cttcggccag gagacgccgg
     9601 gtgaccacct cggccggagg cccgtcggcg gcgacgcgcc cctcgcgcag cgcgacgatc
     9661 cgatcggcga accgcgcggc gtggttgagg tcgtgcagca ccatcacgac cgtcaggcgg
     9721 cgttcggcgc gcagccgtac gaccgtctgg agcacttcca gttggtgccg cagatcgaga
     9781 taggtggtcg gctcgtcgag aaagagcacg cgggcgtcct gggccagcgc catggccagc
     9841 cggacccgct ggcgttcacc tccggacagt gcgtccacgg gccggtcggc ccaggcggtg
     9901 acgccgacgt ccgcgagcgc ccgggacgtc acctcgtcgt cgccctcccg cagcattccg
     9961 agcggccccc gggcggcata gcgcccctgt cgcacgagct gacgcacggt cagccccggt
    10021 acggtgggag ccgactgatg gagcagggcg acgcgccggg cggcggtgcg gcgggaaagc
    10081 cgcgtcacat cgtcgccttc gagcaggacg cggccgccgc tcggggtgag cagtccggcg
    10141 gcgagtttga ggaaggtgct cttgccgcag ccgttcagcc cgatcagggc aaccagctcg
    10201 ccggcccgga cgctcaggtc gaccccgcgc aggaccatgc gtcccgggta gccgaaggcc
    10261 agcccctccg cccggacgac ctcagggccc ggcctcccgc tcggttccgc atccggcccg
    10321 gccgcctcgg tcgggctcga ctccacttgt ccggtcaggc cttccgccat gccgccctcc
    10381 tcatccgttc tcctccactt ccgtcgacga agtcgacggc gtcgccgatg tcgccgatgt
    10441 cgccgatgtc ggcgcgcccc ggccacgtac cgctctccgt catcgctcgt ccgcccgtcg
    10501 ccgtgccacg accatcagca gccccgcccc cacgaaggtg gtcagggcgc cgaccgggag
    10561 ggtcagccgg tccgtgtcga gcgcggtggc cagcagtcgg gacagcagtt gggcggcggc
    10621 atccgccccg cacaccaccg tcgctccgag cagcgcggcc cccggaaggg tgatgcgcag
    10681 gtcggccccg aagacggcca gcgccagatg gggcaccaac agccccacga aacccagcgc
    10741 gcccacggcg gacacggcgc cggccgtcag tgccacggcg cagacgagcg ccgcaccccg
    10801 gccccggccg gtggccagac ccgcggcgga ggcctgttcg tcgccgcagc gcaacagggt
    10861 cagcggcccg gcgagcagcc aggcagccgc gccccagaag gcggcccacg gccagagcag
    10921 actccagtgc tgccacaccc ggccctcggt ggaaccgatc aaccactgga cgacgctgcc
    10981 gagttcaccg ggggccacca ggaggacgac cgccgtgagc ccggcgagca ccgccgagac
    11041 gaggaccccg tagacggccg tctgttccgg gtcgcccgtc tcccgtccgg cgagcagcca
    11101 cagcaggccc gctccggcga agccgccgat gcaggccgcc acgaccacgg ccgtggggga
    11161 ttcccagccc gcgaggccga ggccggtcgc ggcgaccgcg ccgagcaccg cgccgggggt
    11221 gacgccggtg acctcgggtc cggccagcgg gttgcgcagg gccgactgga gcaccagtcc
    11281 ggccaccccg aggcaggccc cggcgaccag cgccaccagc agccggggca accggagctg
    11341 gagcacgatg tgttcggcgg tggggtcg
//
