LOCUS       NZ_CP015885            12151 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Enterococcus faecalis strain sorialis plasmid Efsorialis-p2,
            complete sequence.
ACCESSION   NZ_CP015885
VERSION     NZ_CP015885.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55548)
  AUTHORS   Fouts,D., Sanka,R., Singh,I., Gill,J., Llorente,C. and Schnabl,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2016) JCVI, 9714 Medical Center Drive, Rockville,
            MD 20850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Assembly Name :: HGAP assembly of Enterococcus faecalis sorialis
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10.44x
            Sequencing Technology             :: Pacbio RS II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 02:48:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,936
            CDSs (total)                      :: 2,860
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 76
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 26 of 59
            Pseudo Genes (internal stop)      :: 18 of 59
            Pseudo Genes (multiple problems)  :: 14 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:14:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 37486
            Orig. end                         :: 49637
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015885.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12151
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12151
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12151
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:7151](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7151
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(167..180)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 21.38"
                     /tool="antismash"
     misc_feature    complement(745..760)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.01"
                     /tool="antismash"
     gene            1426..2154
                     /locus_tag="A6B47_RS15180"
                     /old_locus_tag="A6B47_14730"
     CDS             1426..2154
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368105.1"
                     /locus_tag="A6B47_RS15180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14730"
                     /product="hypothetical protein"
                     /protein_id="WP_033624755.1"
                     /transl_table=11
                     /translation="MTRLEQAQGKLQRLKRESEETHRLIRAEHDRIPFGQPNIIGRGDI
                     YKKVNGYHDRAIKLLKEQEKQEKRVEMLEKVEDFKEKNELIKDVHVVGKSSYATVGAKT
                     SVNNIDYFKNELKELEKANEKAKAYNKTKPAIKARTYGAAITKLKNKIATLEQMKEADE
                     NKVMSEKTKELIESGAVTQWKKKPIFYFVKGLRKVALEIDENGDFFISNYYPACTDADK
                     EFINKLLDPAAESTKKETFC"
     gene            2341..2775
                     /locus_tag="A6B47_RS15185"
                     /old_locus_tag="A6B47_14735"
     CDS             2341..2775
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393005.1"
                     /locus_tag="A6B47_RS15185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14735"
                     /product="hypothetical protein"
                     /protein_id="WP_236918842.1"
                     /transl_table=11
                     /translation="MRRLSNTTMANETLKKEAWYRMMLTDLSSSVIDEFMETGKCHYTS
                     NYFQGENILVTEEIESIIKTAEKRYGFLVYYVTENKTADGQRFLSLFYVAPETNDWLYC
                     HRDLESYRQYVYVVNTTNPAFSEFGMIQFDPILGSLLRTS"
     gene            2861..3199
                     /locus_tag="A6B47_RS15190"
                     /old_locus_tag="A6B47_14740"
     CDS             2861..3199
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387966.1"
                     /locus_tag="A6B47_RS15190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14740"
                     /product="hypothetical protein"
                     /protein_id="WP_065536088.1"
                     /transl_table=11
                     /translation="MSMEIRELTIIEDPCFYQYTTIKELESFNTSELTIALSVVRKEQR
                     RLKEKVYHSGSGDQVDKNNVKLINKYRANLEKLLFIKQGYIPDAITQNMIDTLIKNNKK
                     SIQKIKKG"
     gene            3249..3422
                     /locus_tag="A6B47_RS15525"
     CDS             3249..3422
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393256.1"
                     /locus_tag="A6B47_RS15525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002393093.1"
                     /transl_table=11
                     /translation="MAEKIDLNKLYHESKKERTEKQTKMERALEGVVCGLPRNTADDTI
                     LKKRMDQENTNG"
     gene            3415..>3495
                     /locus_tag="A6B47_RS15865"
                     /pseudo=""
     CDS             3415..>3495
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393257.1"
                     /locus_tag="A6B47_RS15865"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DUF2786 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDEKRKDKIQRLLALANDEESMTALAK"
     gene            3666..3878
                     /locus_tag="A6B47_RS15195"
                     /old_locus_tag="A6B47_14745"
     CDS             3666..3878
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360757.1"
                     /locus_tag="A6B47_RS15195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14745"
                     /product="hypothetical protein"
                     /protein_id="WP_002360757.1"
                     /transl_table=11
                     /translation="MKKYKELKEVEIQAISGGVAVTPPKGAFPNGDFKPWNVPSGTTFP
                     WQGACPVGSVTAGYIGGGQNLCRRP"
     gene            3975..4952
                     /locus_tag="A6B47_RS15200"
                     /old_locus_tag="A6B47_14750"
     CDS             3975..4952
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S41"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414729.1"
                     /locus_tag="A6B47_RS15200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14750"
                     /product="S41 family peptidase"
                     /protein_id="WP_010774977.1"
                     /sec_met_domain="Peptidase_S41 (E-value: 2.5e-23, bitscore:
                     74.5, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIFKYLFTLGCLFFLGIYILHQSNQYLSLDTRSVDAVQIMSQSG
                     IIENQKKWASTLSNYNYGKSVKNISELNNLLIRGNKHSSILNVSAESMESDLNTKENLP
                     SSMEIEGLSVISVPGLYTTNNEFRNNYSNTLAKLIDSAKGDIVLDLANNSGGDVVPMII
                     GASSLIPTGKILNSIDKNGNKFPIYLESNKLFGGITNYLEDSSKQLKTQKYSFKKSKKV
                     SVIISDRTASAAEVLTLVLKTNPNVTVLGTPSAGYTSWNETAVLPNKDNPSNFWYMIYT
                     AGYFETIKNHEVFNNTKIIPDVEVRSAYLDIANKQLIEAIRRIK"
     gene            5001..7151
                     /locus_tag="A6B47_RS15205"
                     /old_locus_tag="A6B47_14755"
     CDS             5001..7151
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393091.1"
                     /locus_tag="A6B47_RS15205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14755"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_002393091.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 8.7e-48, bitscore:
                     153.6, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 5.8e-263, bitscore:
                     866.5, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKIIYQQDEKDCGVACVAMILRHYKSEIPIHKLRELSGTDLEGT
                     SALGLKKTLEKIGFDSPAIQADNEVWKEKDLPLPLIAHVLIDNKYMHYVVIYEIKGETL
                     LIADPDPTKGKIKKTIKEFSKEWTGILLLPTPKESYSPTREKVAGLSSFLPIIWRQKGL
                     VFHIVLASLFITLFGIGSSYYFQGLLDYFIPNQAKSTLNIVSMGLIVVYLFRVLFEYSR
                     SYLLVVLGQRMSMAVMLQYFKHVLTLPMNFFATRKSGEIISRFLDANKIIDALASATLS
                     VFLDIGMVLLVGITLAIQNGTLFLLTLASLPFYVVAILAFVKSYEKANQEEMAAGATLN
                     SSIIESLKGIETIKAYSGEEKAYDRVDREFIKLMKKSFRTVTLDNVQQGIKHTIQLISS
                     ALILWLGSFYVMEGKISLGQLITYNALLVFFTDPLQNIINLQVKMQTAQVANKRLNEIF
                     AIEPEKTNDSSFGEISNQIFREGITLKNVSFSYNMKAPTLNNISCTIPPKSKIALVGVS
                     GSGKSTLAKLLVNFYAPSEGVIRYGKMNHLDIPFKQLRKHVTYVPQESFFFSGTIVENL
                     TFGLQEIPSFERIIEVCEAVQLSTFIEQQPLRYDTILEEGGTNLSGGQRQRLALARALL
                     KNADILILDEATSGLDTLLEHTILDYLLTLEDKTILLIAHHLPIAKACEQILVLHEGKL
                     AEQGTHDELRYNQGIYQRLWDI"
     gene            7170..8537
                     /locus_tag="A6B47_RS15210"
                     /old_locus_tag="A6B47_14760"
     CDS             7170..8537
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360753.1"
                     /locus_tag="A6B47_RS15210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14760"
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="WP_002393090.1"
                     /transl_table=11
                     /translation="MEQKDWLDQSSIYSQQHHTFYRWVLYPVILFLCLGGFFLVFAKKE
                     IVIRTPAQLTAQKVEKLQVPIEAKIKANNLKENQTVKKGELLVTFDTTSLQNEKTQIEQ
                     ENTTIEQQKSAAQIFIDSLMSEQNLFETEDDFGYSNQVKSFLAEKEATLYALKQSEVAT
                     QKEQETYDKAKEQLEKQFTMRQKEQRELEQVRTAWVDEQSLEGFSPEITSKYQLWKVQL
                     TDVPEEQKKQVKATILATIDELITQKKKEIEQLQAEQAKLVIPSTIENEINSQKEKGKQ
                     SKELALATAKEKVAELTETQKKNDIALKTINEQLTQSALKAPITGTIHLNAEVENQLEV
                     PKGTTLAEVYPEHKKSELRFTALLPTSESMRVKPGMDVHFKLDKKGVATKTINGTLKEI
                     SENSTTTDQGTFYTIKGILNPSDNFTSRYGLTGELSLIVGKKTYWQEIKDMLFNVN"
     gene            8914..9252
                     /locus_tag="A6B47_RS15215"
                     /old_locus_tag="A6B47_14765"
     CDS             8914..9252
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393088.1"
                     /locus_tag="A6B47_RS15215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14765"
                     /product="hypothetical protein"
                     /protein_id="WP_010830137.1"
                     /transl_table=11
                     /translation="MKSVVWIYTVNTDGVVIRSSGSGMMWISSKKFQDKLKKELLPEWN
                     LSVISYDIVKNDMPDADIIMYNEIDMAYLDEKIKNTGLPVSYIDLQTKNADNIKKKLEN
                     FAKNKISK"
     misc_feature    complement(8925..8943)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.01"
                     /tool="antismash"
     gene            9620..10234
                     /locus_tag="A6B47_RS15220"
                     /old_locus_tag="A6B47_14770"
     CDS             9620..10234
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A6B47_RS15220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A6B47_14770"
                     /product="hypothetical protein"
                     /protein_id="WP_025188019.1"
                     /transl_table=11
                     /translation="MGYVGKSRSVRSQIAIDNAEVPLNHITKDYILTFVTENNIDETLK
                     NESVAMWKFVAKRHGSTSWHHVSKHYNKIDHYDLHDVAEYFSMNYDSLKNDYQNLLDQK
                     RQAKNDLIKNLKLGIIKVQIWGGTKRYPKLEGYESVMGVVKDGWLHTVTLSNQTKYKIT
                     GNKIEEITIFELDQYDILTKKFPEFRAMKRKINKEVARLSK"
     gene            10248..10577
                     /locus_tag="A6B47_RS15225"
                     /old_locus_tag="A6B47_14775"
     CDS             10248..10577
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A6B47_RS15225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A6B47_14775"
                     /product="hypothetical protein"
                     /protein_id="WP_002401839.1"
                     /transl_table=11
                     /translation="MAILNNKGEKISIDCADLISEVEEDILIFGGTFLVYAICSWREIE
                     QVEYISDYVHADNPESYKDELTTKEYAELKEIYEKDLEELKITKNKQMNLNELLSILTI
                     QNSIT"
     gene            10574..11095
                     /locus_tag="A6B47_RS15230"
                     /old_locus_tag="A6B47_14780"
     CDS             10574..11095
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A6B47_RS15230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A6B47_14780"
                     /product="hypothetical protein"
                     /protein_id="WP_002401838.1"
                     /transl_table=11
                     /translation="MKGLLELSTIDLFLKKYGITRNKVATQNIKHKISNNALAQANLRP
                     VETYSVKLILGLSEAVNEAPEKVMAQLLEIEKSQTNSESAQKKEAYQFGNIILEGILNT
                     NRSTHEIRLVQYLGKRTLFCTYVSGVGAMNWSVSDYKEIAETLKIDDVDIRFRTSENDQ
                     FWDVSESYRY"
     gene            11184..11498
                     /locus_tag="A6B47_RS15235"
                     /old_locus_tag="A6B47_14785"
     CDS             11184..11498
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A6B47_RS15235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A6B47_14785"
                     /product="hypothetical protein"
                     /protein_id="WP_002401837.1"
                     /transl_table=11
                     /translation="MTHETSNGNKGLNIVNSGVGVSKKIVDPYIKDGAITIVDSKGYLN
                     TEDLEAKGYEVKTVSLEKDKCTLFDPYQALQELAGEDPIKFKRVVDELEGIIKRITKEN
                     "
ORIGIN
        1 acaaatatag aaccttgata gaatttgctc agaaaataaa tgctcataaa ttagcatatg
       61 atgttatgag tggtgcagta gacatgcgaa tacttacaaa gaactgttta aggaagtaca
      121 aaacattttt aaagaataca gacaatagtt taaaagagat acagctgaaa caaacatttg
      181 aaagcttaaa cataccaatg gtaaaaggga ttgaaaaata cgtattaaag agcgacatat
      241 ccgattttcc aaatgagatt ggcgctgtta agtttcagaa ttggttagta aagcaaggaa
      301 agtcatttaa atattatcaa gactacctca atatgttaac gctcctaaaa atagaaataa
      361 acaaacgtaa tcagttaccg ccagatttag aagtcgcaca cgattgtgca gtggatagaa
      421 ttaatcaggt gaactatgaa gaaagggata aagaaataaa agaacgtttg gaacaattaa
      481 ggaagtatga aagagatatt gatgggtaca catttgtgtt acctaaacga gcaaacgaca
      541 taaaaaaaga aggaaaagca ctaaaccatt gcgtagcaag ctatataagc agacacgcaa
      601 agggagaaac aacgattatc ttcgtaagag agaagaagaa ccctcaaaaa tcttatttca
      661 cattagagta caactacaat agggttgttc agttacaagg aaagaagaat cgacaaaagg
      721 taccagatga attgaaacaa gcggtagaca aatgggtcaa ggtcattaaa gattaaacgt
      781 taggaatgct aagaaaagac cgttcttagc attcctaacc gaaatctagg acaaaatcca
      841 ttgttccgcg gcggtgcagt cgcccttgct ccaaaaccaa tggattttct gctgcactca
      901 acacgagtca attaaacgtt ctattcccat tcgtgccgat tggggaacgt ttctcttttc
      961 ctactcgttc ctcgcttaac ggaaaaggac attgacacga gttgttcctt gtgagaccac
     1021 aaggaagcgt tccgcagatt taagtttttt ctgaagcagt ttcactgcta cagaaaaaag
     1081 gcgttggttc tatgccattt ttctgtcact cggccccttc ttgcggttat catttcttca
     1141 gtgagccggc cttgtgtact tgcgaatcat acaacagcca gtcgaaaaca caagggtaaa
     1201 atgcacgagc aagctcgccc ttgtgttttc tttgtgtctg atgtctgttc tctgcgcacc
     1261 caaaacctca aactcactga agaaatggat cataacagca aacaaagaaa ggagaggacc
     1321 acagaaaaat accgaaccaa cgaagttcaa aagaagcacc ccttcctaaa tcttcaaaaa
     1381 aaatgagttt tgaaaaaaat aaaaaacaga aagaagcgag taaaaatgac tagacttgaa
     1441 caagcacaag ggaaattgca acgactaaaa agagaaagcg aagaaacaca ccgcttaatt
     1501 agagcggaac atgaccgtat ccctttcgga cagcctaaca ttattggccg tggggacatt
     1561 tataagaaag taaatggcta tcatgatcgt gcaattaaat tactgaagga acaagaaaaa
     1621 caagaaaaaa gagtggaaat gctggaaaag gtagaagact ttaaggaaaa aaacgagcta
     1681 attaaagacg ttcatgtggt cggtaaatca agttatgcga cggttggcgc aaaaacaagc
     1741 gtcaataaca ttgactactt taaaaatgaa ctaaaagagc ttgaaaaagc caacgagaaa
     1801 gcaaaggctt ataacaagac gaagcctgcc attaaggcga ggacctacgg cgcagcaatc
     1861 acgaaactaa aaaataaaat tgcgaccctg gaacaaatga aagaagcgga tgaaaataag
     1921 gtgatgagtg agaaaacaaa agaactgata gaaagcggtg cagtaacaca atggaagaaa
     1981 aagccgatat tctatttcgt taaagggttg cgaaaagtag cactagaaat tgacgaaaat
     2041 ggagatttct tcatttctaa ctattatccg gcttgtacag atgcagataa ggaatttatc
     2101 aataagttat tagatcctgc agctgaaagc acaaaaaaag aaaccttttg ctgaagacaa
     2161 aagatttcta ggacgttaac tatttatagt catgaataag tatagcgtac cttagaaaaa
     2221 tattcaatgg gttcctgcca taacgatgga gttatggcaa ggattcattg caactcatag
     2281 aatagaacaa ttatctaaca gtaacgaatt aaaaaaaata ttgcttttgg aggaatacaa
     2341 atgagaagat taagtaatac gacaatggcc aacgaaactc taaaaaaaga agcatggtat
     2401 agaatgatgc ttaccgacct atcaagttct gtcattgatg agttcatgga aacagggaaa
     2461 tgccattaca ccagcaatta ctttcaaggt gaaaatattt tggtcactga agaaatagag
     2521 tcaatcataa agacggccga aaaaaggtat ggcttcctgg tatactatgt aactgaaaat
     2581 aaaactgcag atggtcaaag gttcttgtct ttgttctacg ttgcgccaga aacaaatgat
     2641 tggctttact gtcacaggga tttagaatct tataggcaat atgtgtatgt agttaatacg
     2701 acgaatcctg cattttcaga atttggaatg attcaatttg atccaatact aggcagtctt
     2761 ttacgaactt catagataaa aaaatataca ttttatggta tcagttgggc tttttggccc
     2821 agctgaagcc taacgtaagg aattataaga aaggtggaaa atgagcatgg aaatacggga
     2881 attaaccata atagaagatc cttgctttta tcaatatacg acgataaaag aacttgaatc
     2941 gttcaatacg tctgaattaa cgattgcgct tagcgtagtt agaaaagagc agcgtcgctt
     3001 gaaagaaaaa gtgtatcatt ctggatctgg cgatcaagtg gataaaaata acgttaaatt
     3061 aatcaacaag tacagagcca atcttgaaaa actgctgttt attaagcaag ggtatattcc
     3121 ggatgcaatc acccaaaaca tgattgatac attgatcaaa aacaataaaa aatccataca
     3181 gaagattaaa aaaggttgag tttagcaagg caatagaaac aattttaata gaagagagga
     3241 tgtaaaacat ggctgaaaaa attgatttga ataagttata tcatgaaagt aaaaaggaaa
     3301 gaactgaaaa acaaacgaaa atggaacgag cgttagaagg agtcgtgtgt gggcttccaa
     3361 gaaacacagc tgatgataca attttaaaga aaagaatgga ccaggagaat accaatggat
     3421 gaaaaaagaa aagataaaat tcaacggttg ttagcactag caaacgatga agaatcaatg
     3481 acagctttag caaagcgata agaatttagt aaaggtcctg tgtaagttag ttttgatatc
     3541 gtatactaca agaaataaat ttacctttag ttcttattga cagatacaca taggtttggt
     3601 ttaattaaat tagctttatg aatccgtttt cattttgctg taagaataaa taggaggaat
     3661 gtagaatgaa aaagtacaaa gaattaaaag aagtagaaat tcaagcaatt agtggaggag
     3721 tagcagttac ccctcctaaa ggagcttttc ctaatggaga ttttaaacct tggaacgttc
     3781 cgtcaggaac gacttttcca tggcaaggag cttgtcctgt agggtctgtt actgcaggtt
     3841 atattggcgg tggacaaaat ctttgtcgga gaccataatt aatattaaaa ctattattat
     3901 aaatagtaat tacaatggga acagagatag attattctac ctctgttccc attgtaatta
     3961 tggggaggtt tgttatgaaa atatttaagt acttatttac tttaggttgc ttattttttt
     4021 taggtatata tattctacac caatcaaacc aatatttatc gttagacact aggagtgtag
     4081 atgctgtaca gatcatgagt caatctggaa ttattgaaaa tcaaaaaaaa tgggcctcga
     4141 ccttatctaa ttataattat ggtaagtcag taaaaaatat atcggaacta aataacttat
     4201 taattagggg taataaacat agctcaatat taaatgtttc tgctgaaagt atggaatcgg
     4261 atttaaatac taaggaaaat ctgccatcaa gtatggaaat agagggtctc tcagttatta
     4321 gtgtaccagg tttatatact accaataatg aatttcgaaa taattacagt aatacactag
     4381 ctaagttaat agattctgct aaaggggata tagtattgga tttagctaac aattctggag
     4441 gggacgttgt tcccatgatt ataggggcta gctctttaat acctactgga aaaattttaa
     4501 attcaataga taaaaatgga aataagttcc ccatttattt agagtctaat aaattatttg
     4561 gtggtataac aaattattta gaagacagtt ctaaacaatt gaaaactcaa aaatattcct
     4621 tcaaaaaaag taaaaaagtt tctgttatta tcagtgatag aacagcaagt gcggcagaag
     4681 tattaacatt agttttaaaa accaatccta atgttacagt attaggaaca ccttctgcag
     4741 gttatactag ctggaacgaa accgctgttt taccaaataa agataatcct tcaaactttt
     4801 ggtatatgat atatacagct ggttattttg aaactataaa gaatcatgaa gtgtttaata
     4861 atacaaaaat aattccagat gtagaagtta ggtcggctta tttagatatt gctaataaac
     4921 aacttataga ggctatcaga cgcattaaat aaattacaat agtagctatt aagaataact
     4981 ttaataaaat agaggatgtt atgaaaaaaa ttatttatca acaagatgaa aaagactgtg
     5041 gtgttgcttg tgttgctatg atcttaagac actataaatc agaaattcct attcataaat
     5101 tacgtgaatt atcagggaca gatttagaag gaacttctgc acttggttta aaaaaaacct
     5161 tagaaaaaat tggctttgat tctcctgcta ttcaagccga caatgaggtt tggaaagaaa
     5221 aagatttgcc cctacctcta attgcgcacg tgctaatcga taataaatac atgcattatg
     5281 ttgttatata tgaaataaaa ggggaaacat tattgattgc cgatcctgat ccaacgaaag
     5341 gaaaaatcaa aaaaacaatt aaagagtttt ctaaagagtg gacaggtata ttacttttac
     5401 caacaccaaa agaatcgtat tcgcctactc gagaaaaagt ggcaggatta agttcttttt
     5461 tgcccattat ttggcgacaa aaaggattgg tgttccatat cgttttagct tctctcttta
     5521 ttactctttt tggtattggt agttcttatt attttcaagg actgttagat tactttattc
     5581 ctaatcaagc aaagtccacg ttaaacattg tttcaatggg acttattgta gtctatttgt
     5641 ttcgtgtatt attcgaatat agtcggagtt atctcttagt tgtattgggt cagcgtatga
     5701 gtatggctgt gatgcttcaa tacttcaaac atgtactcac gttaccaatg aactttttcg
     5761 caacccggaa atcaggagaa attatctctc gctttttaga tgcaaataaa attatcgatg
     5821 cacttgccag tgctacttta tctgtttttt tagatatagg tatggtgcta ttggtaggaa
     5881 ttaccttggc gattcaaaat ggaacgttat tcttactcac tttggcttca ttgccctttt
     5941 atgtagtcgc tattctagct ttcgtcaaga gctatgagaa agccaatcaa gaagaaatgg
     6001 ctgctggtgc taccctaaat tcaagtatta ttgaaagctt aaagggtatt gaaactatta
     6061 aagcctatag tggggaagaa aaagcatatg atcgagtaga tagagaattt atcaaattaa
     6121 tgaaaaaatc ctttcggacg gttaccttag acaacgtaca acaaggaatt aaacatacta
     6181 ttcaattaat tagcagtgca cttattctgt ggttaggttc tttttacgta atggaaggga
     6241 aaattagcct tggtcagctt attacttaca atgcattatt ggttttcttc acggatccct
     6301 tacagaatat tatcaacttg caagttaaaa tgcagacagc gcaagtagct aataagcgct
     6361 taaacgaaat atttgcaatt gagccagaga aaacaaatga ttcttcattc ggtgaaattt
     6421 ccaatcaaat atttcgagaa ggaattactt tgaagaacgt atccttttct tataacatga
     6481 aagcaccaac gttaaacaat atttcttgca cgataccacc taaaagcaaa attgctttgg
     6541 taggagtcag tggttcgggg aaatcaactt tggccaaatt actggtcaat ttttatgctc
     6601 cttcagaggg agttattcgt tatgggaaga tgaatcattt agatattccg tttaagcaat
     6661 tgcgaaagca tgttacctat gttcctcaag aatccttttt cttcagtgga acgattgttg
     6721 aaaatttaac ttttggctta caagaaatcc ctagttttga gcgaattatc gaagtatgtg
     6781 aagcggttca gttaagcaca tttattgagc aacagccttt acgttatgat acaattttag
     6841 aggaaggggg cactaatctt tctggaggtc aaagacaaag attagcgctt gcaagggctc
     6901 ttttgaagaa tgctgatatt ttaattttag atgaagcaac cagtggttta gatacattat
     6961 tagagcacac cattcttgac tatttactaa cgttagaaga taaaactata cttttaattg
     7021 cccatcattt acctatcgct aaagcttgcg agcaaatttt agttcttcat gagggaaaac
     7081 tagccgaaca agggacccat gatgaactac gctataatca aggaatatat cagcggttat
     7141 gggatattta ggaaaggact ggaggatcta tggagcaaaa agattggttg gatcaatcaa
     7201 gtatttatag ccaacaacac catacctttt atcgctgggt tttatatcca gtgattcttt
     7261 tcctttgttt aggtggtttc tttttagttt ttgccaaaaa agaaattgtg attcgaacgc
     7321 ctgctcaact gacagcacaa aaggtagaaa aactacaagt accaatagaa gcaaagataa
     7381 aagcaaataa tctaaaagaa aaccaaacag taaaaaaggg tgaactatta gtaacctttg
     7441 atacaacctc cttacaaaat gaaaaaacac aaatagaaca agaaaatact actattgaac
     7501 aacaaaagag tgctgcacaa atatttattg atagtttgat gagtgaacag aacttgtttg
     7561 aaacagagga tgactttggt tacagtaatc aagtaaagag ctttcttgcc gaaaaagaag
     7621 cgacgctcta cgctttaaaa caaagtgaag tagctacaca aaaggaacaa gaaacatacg
     7681 ataaagccaa agaacaatta gagaaacagt ttactatgcg tcaaaaagaa caacgagaat
     7741 tagaacaagt acgaacggcg tgggttgatg aacaatccct agagggattt tcacctgaaa
     7801 tcacttctaa atatcagctg tggaaagttc agttaactga cgtaccagaa gaacagaaga
     7861 agcaggtaaa agcaacaata ttggccacaa ttgacgagtt gatcactcag aaaaaaaagg
     7921 aaattgaaca attacaggca gaacaggcaa aattagtaat acctagcacg attgaaaatg
     7981 agataaatag ccaaaaagag aaaggaaaac aaagtaaaga gttagcttta gcgacagcta
     8041 aagagaaagt agcagaacta actgagaccc aaaagaaaaa tgatatagcg ctgaaaacaa
     8101 tcaatgagca gctcacacaa agtgcattga aagcaccgat tacgggaacg attcatttaa
     8161 acgcagaagt agaaaaccag ctggaagttc ctaaaggaac gacgcttgca gaagtttatc
     8221 cggaacataa aaaatcagaa cttagattta ccgcactctt acctactagt gaaagtatga
     8281 gagttaaacc aggaatggac gttcacttta agctagataa aaaaggtgtt gccactaaaa
     8341 caatcaatgg tacgctaaaa gaaatttcag aaaacagtac aaccactgac caaggaacat
     8401 tttatactat aaaaggaatt ctaaatccat ccgataattt taccagtcgt tatggtctta
     8461 ctggtgagtt atcccttatt gttggaaaga aaacttattg gcaggaaata aaagacatgc
     8521 tttttaatgt aaattagata ttagagttta ctttttttga attgaatatt ttaaaaggtg
     8581 gggagatagt atgaagaaga aagatgtttt aaaaccaacc aatcttttaa tatttttgag
     8641 gctagttctt ttacaatttt gtcaagatct ttattataat ggattttatt tatcaaagta
     8701 gtagttgtta ttgcaggtcg atatcgtaat ttttttgata atggctctct cagaaaaatt
     8761 gtactattgg tttaattaag aaaaaaacta aaaatcagat caatattttt tgagaaatct
     8821 aatcagcaat ttaccagatg aacagctaat tgtttttcgt tggttgcaga ctaaatatag
     8881 tagattttat tatagtttaa ggagagaata aatatgaaaa gtgtagtatg gatttataca
     8941 gtaaatactg atggagttgt gattaggagc agtggttctg gtatgatgtg gattagctca
     9001 aaaaaatttc aagataaact aaaaaaagaa ttactaccag aatggaattt atccgttatt
     9061 agttatgata tagttaaaaa tgatatgcct gatgcagata ttattatgta caatgagata
     9121 gatatggcat atcttgatga aaaaataaag aacactggat tgcctgtatc atatatagat
     9181 ttacaaacaa aaaatgctga taatataaag aaaaaattag aaaactttgc taaaaataaa
     9241 atatcaaaat aactaccata agttactgat agaaaagaac caacagagta tgccttgttg
     9301 gttcttttct ttgtccagtt gttaccaggt cagtagtagg acattcaaat tgggcatacg
     9361 tcatttgtgt taattttgag tacgctttaa atttacatgt aatgaataaa gtaatgggta
     9421 ttcgttttcc actaactggc caaacagata gataggtgaa gaacaaattt aacgcaaatg
     9481 gtaatgattg tgtttacatt taccttatat gtgatataat ataagtgtaa tcaaagaagc
     9541 ctactcttga aaattcaaga ataggcaggt cgctaaacct ctttgattat accatatatc
     9601 aaaggaagaa ggaatgaaaa tgggatacgt aggaaaatca agaagtgtaa gaagtcagat
     9661 agcaattgat aatgcggagg ttccgttgaa tcacattacg aaagactaca ttttaacgtt
     9721 tgttactgaa aataacatcg atgaaacatt aaaaaatgaa tctgtagcaa tgtggaaatt
     9781 tgttgcaaaa agacacggat caactagttg gcaccatgtt agtaaacact acaacaaaat
     9841 tgatcattat gatttacatg acgtagcgga atatttctct atgaattatg atagcttaaa
     9901 aaatgattat caaaatttat tagatcaaaa aaggcaagca aagaatgatt taattaaaaa
     9961 tcttaaatta ggtattatta aagttcaaat ttggggagga acgaaaagat accctaaact
    10021 tgaaggatat gaaagtgtta tgggagtagt taaagatggg tggttgcaca ctgtgacact
    10081 ctcaaatcaa acaaagtata aaattacagg aaacaaaata gaagaaatca ctatttttga
    10141 attagatcaa tacgatattt taacgaaaaa atttccagag tttagagcaa tgaaaagaaa
    10201 aatcaacaaa gaagtagcac gattaagtaa gtaaaggagg aatttgtttg gctattttga
    10261 ataataaagg agaaaagatc agcattgatt gtgctgacct aatatctgaa gtagaagaag
    10321 acattctcat ttttggtggt acgtttctag tctatgctat atgctcttgg agagaaatag
    10381 agcaagtaga gtatatttct gactatgtac atgcagataa tcctgaaagt tataaagatg
    10441 agttaacaac aaaagagtat gcagaattaa aggaaattta tgaaaaggac ctggaagaat
    10501 taaaaattac aaaaaataaa cagatgaatc tgaatgagct actatctatc ttaactatcc
    10561 agaactcaat aacgtgaaag ggctgttaga attgtcaacc attgatttat ttttaaaaaa
    10621 atatggtatt actagaaaca aagttgcgac acaaaatata aagcacaaaa tatccaacaa
    10681 tgcgctagca caagcaaact tacgtccagt agaaacgtat tcggtaaaac tgatcttagg
    10741 gttatccgaa gcagtaaatg aagcaccaga aaaagtcatg gcacaattac tagaaattga
    10801 gaaaagtcaa acgaattcag aaagcgctca aaagaaagaa gcctatcaat ttgggaatat
    10861 tattttagaa ggtattctaa ataccaatag atccactcat gaaattcgcc tagtgcaata
    10921 cttagggaaa aggaccttgt tttgtacgta cgtttctggt gttggagcaa tgaattggtc
    10981 tgtgagtgat tataaagaaa ttgcagaaac actaaaaatt gatgacgttg atattcgttt
    11041 taggacaagt gaaaatgatc agttttggga tgtatcggaa agttatagat attgagacaa
    11101 aagttattaa tctaaatgca aagtattggg gttagtaatt gacctaatta atgatgattt
    11161 aataggaaag gaagtagtaa aacatgacac atgaaacaag taacggaaat aagggcttaa
    11221 acattgtaaa ttcaggtgtt ggagtttcaa aaaaaatagt tgatccttat attaaagacg
    11281 gtgcaataac aatagttgat tcaaaaggtt atttaaatac agaagatttg gaagctaaag
    11341 gatacgaagt taaaacagtt tctttagaaa aagataaatg cactcttttt gatccatacc
    11401 aagcattgca agaacttgct ggagaagatc cgattaaatt taaaagggtt gtagatgagt
    11461 tagaaggcat tatcaaaagg ataacaaaag agaattaaaa tgtttaacaa agaagagaac
    11521 cttttttgta aacaattcac acaataaagc attgaaaatt tacaagaaaa aaagatgcga
    11581 tgaatatgac acaaccttgt tgtgacttat tcaactaaaa ccgacaggtc agaaactttt
    11641 aagtttttga cctatttgtt ttttcgtgct atacttggaa ttatcaagtg tcaaaaactt
    11701 atgttcagaa aaggtgaggg aaaattggcc atttatgggt attcacgagt cagtaccagc
    11761 aatcaagatt acaaaaccca aattcgaaaa ttagaaggag ctggagcaga aaaaattttt
    11821 agtgagaaat atacaggtac aaaaaaagaa ggaagaaaag aattagaaga gctgctttca
    11881 attgtaaaag ctggcgataa agttttggtg acaaaaattg atcgattggc cagaagtatc
    11941 gtcgatttaa attcaatcat ttctgcatta aatcataaag gggtgactat ttctttttta
    12001 gatgatcact taacgtttga acctgataag aaggactcta tgcaaacgtt aatgatgaat
    12061 atgctgggta gctttgcgca atttgaaaga gatttaatcg tgacgagaac gcaagaaggg
    12121 aaacagtggc atcgagcgaa caataaaaat t
//
