LOCUS       NZ_CP015884            10147 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Enterococcus faecalis strain sorialis plasmid Efsorialis-p1,
            complete sequence.
ACCESSION   NZ_CP015884
VERSION     NZ_CP015884.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 125418)
  AUTHORS   Fouts,D., Sanka,R., Singh,I., Gill,J., Llorente,C. and Schnabl,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2016) JCVI, 9714 Medical Center Drive, Rockville,
            MD 20850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Assembly Name :: HGAP assembly of Enterococcus faecalis sorialis
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10.44x
            Sequencing Technology             :: Pacbio RS II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 02:48:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,936
            CDSs (total)                      :: 2,860
            Genes (coding)                    :: 2,801
            CDSs (with protein)               :: 2,801
            Genes (RNA)                       :: 76
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 35 of 59
            Pseudo Genes (incomplete)         :: 26 of 59
            Pseudo Genes (internal stop)      :: 18 of 59
            Pseudo Genes (multiple problems)  :: 14 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:14:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 114556
            Orig. end                         :: 124703
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015884.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10147
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10147
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10147
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5147](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5147
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(286..579)
                     /locus_tag="A6B47_RS15715"
     CDS             complement(286..579)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A6B47_RS15715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_236918838.1"
                     /transl_table=11
                     /translation="MKISAKLFVLGIGLTFGGLITSVPAEEKNIVTSNYELGSFAQNDP
                     VNYSLLRSKRSLSETPPRVDFINISSHNGEISVEQFQKMKNMGIKGVVVKLT"
     gene            complement(789..1391)
                     /locus_tag="A6B47_RS14895"
                     /old_locus_tag="A6B47_14470"
     CDS             complement(789..1391)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821245.1"
                     /locus_tag="A6B47_RS14895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14470"
                     /product="Fic family protein"
                     /protein_id="WP_010821245.1"
                     /transl_table=11
                     /translation="MLENKLGIINQLELNRVEERVSKEKAKQLYDSGDIDRVEIGTFKG
                     LSYIHNYLFEDIYEFAGKVRSQNISKGNFRFAPVMYLEIALEHIDKMPQRNLDEIVAKY
                     VEMNIAHPFREGNGRATRIWLDLILKKELKQVVDWNLIDKEDYLSAMERSPVKDLEIKY
                     LISNALTDKINDREIFMKGIDISYYYEGYTEYNVEDL"
     gene            complement(1637..2575)
                     /locus_tag="A6B47_RS14900"
                     /old_locus_tag="A6B47_14475"
     CDS             complement(1637..2575)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053446.1"
                     /locus_tag="A6B47_RS14900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14475"
                     /product="hypothetical protein"
                     /protein_id="WP_087548801.1"
                     /transl_table=11
                     /translation="MNLRKIEHEIEEILSKDTHSWVRLYELIREVEYSKLWRNEYSSFT
                     QWIKHLAYVTGVTESLIWKRKKAGEIYFDYQQRARSRGVSVPNIEDVEVSPDNFELVEK
                     ISQGNSQIKDELMQQVLAKDIKRSDLVNTWSTIKTIQAKEGGGIVKKNRYSKIDSSDEQ
                     IFTISDFSFALSESSWLQIAKNSSHKGKSVYKLIPNFSFYSSLLMRSVTLDFLLLENVS
                     SKYTQELNTHSIEIVFSDNKLNNIILNTKTNYSWVVVPEDISLIALKQLPKEIGLLKIS
                     SKRIIQVVRNAALTNETSKLDILQTFIVKTI"
     gene            complement(2687..3028)
                     /locus_tag="A6B47_RS14905"
                     /old_locus_tag="A6B47_14480"
     CDS             complement(2687..3028)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /locus_tag="A6B47_RS14905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14480"
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_087548803.1"
                     /transl_table=11
                     /translation="MSDEKKYIPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYEFNRKT
                     MFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQLKSLDFTERKLRQIEHLPLKDMAK
                     IDQIIEYIF"
     gene            complement(3028..3258)
                     /locus_tag="A6B47_RS14910"
                     /old_locus_tag="A6B47_14485"
     CDS             complement(3028..3258)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401161.1"
                     /locus_tag="A6B47_RS14910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14485"
                     /product="hypothetical protein"
                     /protein_id="WP_000958277.1"
                     /transl_table=11
                     /translation="MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVP
                     KLQDPFSGGSEGEYYEKDEWYDIEPGGRELF"
     gene            3462..4082
                     /locus_tag="A6B47_RS14915"
                     /old_locus_tag="A6B47_14490"
     CDS             3462..4082
                     /GO_function="GO:0000150 - DNA strand exchange activity
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367722.1"
                     /locus_tag="A6B47_RS14915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14490"
                     /product="recombinase family protein"
                     /protein_id="WP_168354168.1"
                     /transl_table=11
                     /translation="MFRKGEEILAIYGYSRVSTSNQDYKTQIQKLEKAGAEKIFSEKYT
                     GTKKEGRKELEELLSIVKPGDKVLVTKIDRLARSIVDLNSIISSLNNSGVTITFLDNAL
                     TFEPNKSDSMQTLMMNMLGSFAQFERDLIVTRTQEGKQWHRANNKNYREGRPKRVLNDK
                     YKHALELMETNSMREVEKKTGISLSTLKRIKKQAKEEQLLSEK"
     gene            4121..4321
                     /locus_tag="A6B47_RS14920"
                     /old_locus_tag="A6B47_14495"
     CDS             4121..4321
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862521.1"
                     /locus_tag="A6B47_RS14920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14495"
                     /product="hypothetical protein"
                     /protein_id="WP_087548804.1"
                     /transl_table=11
                     /translation="MVVRKKYDHRGIEITTWNKTNVVAYVDCGQLARRELGKYNHFKCS
                     NCKKEYKLSKGICVEINPEKN"
     gene            4488..4688
                     /locus_tag="A6B47_RS14925"
                     /old_locus_tag="A6B47_14500"
     CDS             4488..4688
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367787.1"
                     /locus_tag="A6B47_RS14925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14500"
                     /product="transcriptional regulator"
                     /protein_id="WP_077933125.1"
                     /transl_table=11
                     /translation="MRKEYDYQKIEVANKAEGVLVEYISCDCGMLAEPERIRWKRTEYK
                     CKSCGKQYKLVFGGQYVEVKN"
     gene            5001..5147
                     /locus_tag="A6B47_RS14935"
     CDS             5001..5147
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_II"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /locus_tag="A6B47_RS14935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class II bacteriocin"
                     /protein_id="WP_077933127.1"
                     /sec_met_domain="Bacteriocin_II (E-value: 2.1e-21,
                     bitscore: 68.6, seeds: 19, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVEAATYYGNGLYCNKQKCWVDWNKASREIGKIIVNGWVQHGPW
                     APR"
     gene            5164..5448
                     /locus_tag="A6B47_RS14940"
                     /old_locus_tag="A6B47_14505"
     CDS             5164..5448
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339190.1"
                     /locus_tag="A6B47_RS14940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14505"
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_077933126.1"
                     /transl_table=11
                     /translation="MDKQQELLDLLSKAYNDPKINEYEGLKDKLFECAKRLTTNETNIG
                     EVCYKLSTTNSEYLARHHFEMPESIIELQKFVTKEGQKYRGWASIGIWS"
     gene            5472..5744
                     /locus_tag="A6B47_RS14945"
                     /old_locus_tag="A6B47_14510"
     CDS             5472..5744
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632800.1"
                     /locus_tag="A6B47_RS14945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14510"
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_087548805.1"
                     /transl_table=11
                     /translation="MDIKKKEALEKIIQLTNKALANPQISSDKKLNGLLLKIREKALSG
                     EVFYDLKKELQSTVSGFTLRNNFQTPGELLDLLALIRTPKGWSGF"
     gene            <6127..>6231
                     /locus_tag="A6B47_RS15720"
                     /pseudo=""
     CDS             6127..>6231
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /locus_tag="A6B47_RS15720"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="transposon DNA-invertase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MIFHLFAKFERNLILKRSAIVRASSRARGRFDDYP"
     misc_feature    complement(6143..6155)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 20.68"
                     /tool="antismash"
     gene            complement(<6483..>6613)
                     /locus_tag="A6B47_RS15725"
                     /pseudo=""
     CDS             complement(<6483..6613)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677538.1"
                     /locus_tag="A6B47_RS15725"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="3-dehydroquinate synthase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDLAGYTAAIFNRGVKFIQLF"
     gene            complement(6907..7035)
                     /locus_tag="A6B47_RS15800"
                     /old_locus_tag="A6B47_14515"
     CDS             complement(6907..7035)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409262.1"
                     /locus_tag="A6B47_RS15800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14515"
                     /product="hypothetical protein"
                     /protein_id="WP_255321410.1"
                     /transl_table=11
                     /translation="MKKLVLGSLLLVALGVIGTSFALTTEVKQEAGYGHLLIDHKI"
     misc_feature    complement(7111..7124)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.1"
                     /tool="antismash"
     gene            7128..7379
                     /locus_tag="A6B47_RS14955"
                     /old_locus_tag="A6B47_14520"
     CDS             7128..7379
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375396.1"
                     /locus_tag="A6B47_RS14955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14520"
                     /product="hypothetical protein"
                     /protein_id="WP_077933104.1"
                     /transl_table=11
                     /translation="MKKNILEDYKATKNKGEDFLHWLLVRKLNTFGKVVIAIILWLLWL
                     KYAFNLVFMVNFLKLIVLITIIYWLVDIYLRVKNKLKK"
     gene            complement(8813..9355)
                     /locus_tag="A6B47_RS14960"
                     /old_locus_tag="A6B47_14525"
     CDS             complement(8813..9355)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016626931.1"
                     /locus_tag="A6B47_RS14960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A6B47_14525"
                     /product="hypothetical protein"
                     /protein_id="WP_077932956.1"
                     /transl_table=11
                     /translation="MAEIKNLGIFGSSNKGLDKIKQRSKEKASETTISNISKTNSTSRS
                     QKTSSVAHESPIRKKRKGAPITKFDKVYAAMQSVKLSALTNATASIMVEKYETNMKKDE
                     LLRKALNEYIRLNLSQEDKNDLLNSVALELDLFRETHPIIPDVDIDNNVIRSPEEIKQE
                     TFTDIKKQWGINDKIRK"
     misc_feature    complement(10024..10033)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 15.06"
                     /tool="antismash"
ORIGIN
        1 ataatggaca acatttttga agccacgttt tgctaattga tctctaaagt agacagaaac
       61 ttttgtggca tctgcgttaa tcattggcgt ggtttctgca tcattaacca ggacactatt
      121 ttgggatagc cctaattctt ctgcgtaatc cgcatagtaa ttggcttgat caattgcttc
      181 ttgttgattt tcaaaccaag aaaaatgata tgttgaaaca gttagacctg cttttttggc
      241 attggtaatt tgactaggtg ctaacgggtt tttgtaggtt gttccttaag ttaatttgac
      301 tactacgcct ttaattccca tgtttttcat cttttgaaat tgttctacag aaatctctcc
      361 attatgagag gaaatattga taaaatccac tctaggaggt gtttcactga ggctgcgttt
      421 acttcttagt aaggaataat tgacagggtc attttgagca aaacttccta attcatagtt
      481 actcgtcaca atattttttt cttctgctgg aacacttgtt ataagacctc caaaagttag
      541 gcctatccct aaaacaaata atttagctga aattttcatt taaccattcc cttcttcttc
      601 tgttgtatgg tacattaaca ggatagatca ttaatgaaaa ataataaatg ttttttatta
      661 ttacatataa aaggttaagt taaacggttt taacaatgaa cgcttgtaag acatataaac
      721 tactagtctc attggtaagt gctgcatgcg taacaacttg aatttttctt taaaatgaag
      781 actttaattt ataagtcctc aacattgtat tcggtgtatc cttcataata atagctaata
      841 tcaataccct tcatgaagat ttctctatca ttaattttat cagttaaagc atttgagatt
      901 aaatatttaa tttccaaatc tttgactgga cttctttcca ttgcagataa ataatcttct
      961 ttatcaatta agttccaatc tacaacctgc tttaattctt ttttcaaaat taagtctaac
     1021 catattctcg ttgctctacc attgccctca cgaaaaggat gtgcaatatt catttcaaca
     1081 tatttggcta caatttcatc taaatttctt tggggcattt tatctatatg ttctaaagcg
     1141 atttctaggt acattactgg tgcaaagcga aaatttcctt tagaaatatt ttgacttcta
     1201 actttacctg caaattcata aatatcttca aacaaataat tatgaatata tgatagcccc
     1261 ttgaatgttc caatttctac tctgtcaata tctccagagt catataattg ttttgccttt
     1321 tctttactga cacgttcctc aactctgttt aactcaagtt ggttaataat acctaatttg
     1381 ttttctaaca actgaattca ccgcctatta attattaatc cttttttcaa taactctttc
     1441 aactttcatg ctaagttttt caatgtattt tagcacgaaa tctacataac aatcaaaaat
     1501 ataaagacac ttgctattcc cattttttca tatggtaaaa ataaactcaa tgccattttt
     1561 gagatatgct gcatcgtcat gatagattat tgatgatgta taataaacac tttttttcta
     1621 catataagat attaatttat atagttttaa cgatgaatgt ttgtaaaata tctaatttac
     1681 tagtttcatt agtaagtgct gcattcctaa caacttgtat tattctttta gatgaaattt
     1741 ttaataaccc tatttcttta ggaagctgtt taagtgcaat gagtgagata tcttcaggaa
     1801 caacgaccca gctataatta gttttagtat ttaaaattat attgttaagt ttgttatccg
     1861 aaaaaactat ttcgatgcta tgtgtattta attcttgtgt gtatttacta gacacgtttt
     1921 ctagcaataa aaaatctaaa gtaacagatc tcatcaataa cgaagaataa aaggaaaaat
     1981 tgggaattag cttataaact gattttcctt tgtgagatga attcttagca atttgtaacc
     2041 acgaactttc tgacaatgca aacgaaaaat cagatatagt aaatatttgt tcgtcactac
     2101 tatcaatttt agagtatcta ttctttttaa cgattccccc accctcttta gcttgaatcg
     2161 ttttaatagt tgaccaagtg tttactaaat cagatcgctt aatatctttt gctaaaactt
     2221 gttgcattaa ttcatctttt atttggctgt ttccttggct aattttttct actaattcaa
     2281 agttatctgg actgacttct acgtcctcta tgttaggaac actaactccc ctacttcttg
     2341 ctcgttgttg gtagtcaaag tatatttctc ctgctttttt tcttttccat attagagatt
     2401 cagttactcc tgttacatat gctaaatgtt taatccactg tgtaaaagaa ctatactcat
     2461 tacgccataa tttactatat tctacttctc ttattagctc gtatagacgc acccatgaat
     2521 gagtatcctt agacaatatt tcttcaattt catgttctat ttttcttagg ttcaatttat
     2581 cctcctttct gtctatttac tataatacgt tatagtaaat agacgttata aaaagaaaga
     2641 attcatgaac attaaatatt atatgtattc tttcttccta ttataattaa aaaatgtatt
     2701 caatgatttg atctatttta gccatatcct ttaatggtaa atgttctatt tgtcttaact
     2761 ttctttctgt aaaatctaaa gattttaatt gggaaataac gacttgtcca tgagtttcca
     2821 tttcatcagg taatgtataa cgagtgggca tatttttaat tgtagaagtt atgggacaaa
     2881 ttactgcaaa cattgtcttt ctattaaact catatctaga aactactaga cctggacgtc
     2941 ttttctgtat ttcttttcca gctgaaggat cgaaatctat ccatacaata tcgccctttt
     3001 taggaatata tttcttttcg tcactcatta aaataattct ctccctccag gttcaatgtc
     3061 ataccattca tctttttcgt agtactcccc ttctgatcca ccactaaatg gatcttgtaa
     3121 tttagggact aacgtaattg tcccatcatc agaatacatc actatatatt cttggtctgc
     3181 ttttggtttc tgtccgttat tagatggtaa tgtcaatact acagaacttc cttgttttct
     3241 agattttgtg gttaacatac aatcacctct tatttttaat tatactaaat aattaattag
     3301 ttttaaatga ttatatacta catttaatta acttagtgta atttagttag ataatcgaga
     3361 ttagatattt tagaattaat tcgtcgggtt cagatactta aaagtttttg acctctttgt
     3421 tttttcgtgc tatacttgga attatcaagt atcaaaaact tatgttcaga aaaggtgagg
     3481 aaattttggc catttatggt tattcaagag tgagtactag taatcaagat tacaaaaccc
     3541 agattcaaaa gctagaaaaa gccggagcag aaaaaatatt cagtgaaaaa tatacgggaa
     3601 caaaaaaaga aggaagaaaa gaattagaag aactactatc tattgtaaaa cctggcgata
     3661 aagttttagt gacgaaaatt gatcggctag ctagaagtat agttgattta aattcgatta
     3721 tctcttcttt aaataattca ggagtgacta tcactttctt agataatgcc ctaacctttg
     3781 aaccaaataa aagtgattct atgcaaacat taatgatgaa catgttaggt agttttgcgc
     3841 aatttgaacg agacttaatc gtaacgagaa cccaagaagg aaagcaatgg cacagagcaa
     3901 ataataaaaa ctatcgagaa ggacgaccta aaagagttct gaatgataaa tacaaacatg
     3961 cactagagtt aatggaaaca aacagtatga gagaagtaga aaagaaaaca ggcatttctt
     4021 tgtctactct taaacgaatt aaaaaacaag ctaaagaaga acaattactt agtgaaaaat
     4081 agataaggaa aagttactat gaaaaataga gagatttaag atggttgttc gaaaaaaata
     4141 tgatcaccgg ggaatagaaa ttactacgtg gaataagaca aatgtagttg cttatgttga
     4201 ttgtggccaa ttggctagaa gagagttggg aaaatataat cattttaagt gcagtaattg
     4261 taaaaaagaa tataaactaa gtaagggtat ttgtgtagaa ataaacccag aaaaaaattg
     4321 aaaaaagagg aaatcttgat aaaatagtaa taggcaacaa ctaattcctc aatattaatt
     4381 attaatataa tagtgtcaat taaagtgcat aaatttatcc cttaattttt agttgttgcc
     4441 ttcatgataa ataaaattgt ggtagttatt atttaaggtg actatttatg agaaaagagt
     4501 atgattatca gaaaatagaa gtagctaata aagctgaagg tgttttggtt gagtatattt
     4561 cttgtgattg tggcatgtta gcggaaccgg aacgtattcg ttggaaacga acagaatata
     4621 aatgtaaaag ttgtggtaaa cagtataagc ttgtatttgg tggacaatat gtagaagtaa
     4681 aaaattgagg tgacagctag tgaaagagaa agaaagtctt tcttatcata catatgaatt
     4741 tttgaaaaaa agaaaacata atccaaaatg gaaagaaaag tatataaagt gaaaagaaga
     4801 acacactata gcatttatgt ataaaatttt ttggttttta gtaggcttga atgttgttat
     4861 ttggacatac ggtttttaca agcattttta attgaagggg taaaaaattg aaattgatca
     4921 ataccaactt acaaaaatgt taggaaaatt cttttgtttt aacatttaca aaactatacc
     4981 aatagttcta taatttctat ataaatgtag aagctgccac gtattacgga aatggtttat
     5041 attgtaataa gcaaaaatgt tgggtagact ggaataaagc ttcaagggaa attggaaaaa
     5101 ttattgttaa tggttgggta caacatggcc cttgggctcc cagataggaa ggattagttt
     5161 aaaatggata aacaacaaga attactagat ttacttagca aagcgtataa tgatcctaaa
     5221 ataaatgaat atgaggggtt aaaagataag ttatttgaat gtgccaaaag attaacaact
     5281 aatgaaacca atatcggtga agtctgttat aaattaagta caactaatag tgagtattta
     5341 gcaagacatc attttgaaat gcctgaatca attattgaat tgcaaaaatt tgtgactaaa
     5401 gaaggtcaaa aatatagagg ctgggcgtct atcggtattt ggagttaaac aatcgttttt
     5461 taggagagtt tatggatatc aagaaaaaag aagcgttgga aaagattatt caattaacaa
     5521 ataaagcatt agcgaatccg caaatttctt cagataaaaa gttaaatggc cttttattaa
     5581 aaataaggga aaaagcttta tcaggggaag tattttatga tttaaaaaaa gagttacaat
     5641 caacagttag tggattcact ctaagaaata actttcaaac acctggtgaa ttattagact
     5701 tactagcgct gatacgaaca cccaaaggat ggtcaggatt ttaaagactt agagttaatt
     5761 catataaagt aatataaact agcatgtatt tgtagtacaa aaaactcact ttcctaacta
     5821 acagtgagtt tttttatttc ttaaacattt tctaactatg tttatgcatc tccaaaagta
     5881 attaaaatat aagagaaatt caactatatt gtatattctg ttacactcta ttttttaaat
     5941 atgagtatat ttagtgttca taaactcgta acaaactcta tgttcaatta tattgtttag
     6001 ataaatttat attacaatag gtttaactac ggagtgattt ttttagaatt ggatacgctc
     6061 gaatatctac cagattacaa aatttagatt tacaaaaagt cagtttaaat aagtctactg
     6121 cctgctcata tattccattt atttgcaaaa ttcgaacgaa atctaatttt aaagcgttca
     6181 gctattgtcc gtgcttcttc tagggctcgt gggcgtttcg atgactatcc ataaaaattg
     6241 tcttcaaaag atttagaatt aatcaaaaca ctcattgata gaagaaccgt ttgattttgt
     6301 gcatatcagt gaagtaaatt atcctaaatc tggcaagcaa atcacatcct aattagatat
     6361 tgaagttttc aatgaaaacg gataaaaaaa acggcaatga ctcacgatca aattagatga
     6421 ctatagcaaa tgtattgcag gatataattt atcgttaggt aagtaatttg tcccgtccct
     6481 attatcatat tgaccaattc ttcaaagtga atagccacct ttccacctat agaactatct
     6541 acttgactta gaaaagttgg ataaatttca ctcctctatt aaatatagct gcggtgtatc
     6601 cggctaaatc gcccctcctc caaaagctaa agtaatgtca ttctatttcc actagcttca
     6661 acaatataca cttcaataaa acttttataa aacggaatat ttacataatt aaagtctaag
     6721 agtaatcaaa ttacttgata aatatgggaa aacaatgatt aattaaaatc acgggaaaaa
     6781 tcttgttact tgactcaaaa tgacaaaaag ggtttttatt aaacaacttt atcatttaat
     6841 acttatttac taatagaaaa aaagacataa cctttataac tatcaaaggc tatgtccttt
     6901 ttgtaactaa attttgtgat caataagaag atgtccatat cctgcctctt gttttacttc
     6961 agtagttaaa gcaaaagatg tcccgataac acctagagct actaaaagca atgaacctag
     7021 tactaatttt ttcataagaa aaatacctcc tttcctttta attgcattaa cattaatata
     7081 taattaaata cacgttaata taaaatgaga caaacagagg tttatacatg aagaaaaata
     7141 ttctagaaga ttataaagct actaaaaaca aaggagaaga ttttttacat tggttattgg
     7201 ttaggaaatt aaataccttt ggaaaagtag ttatcgcgat tatattatgg cttttgtggt
     7261 taaaatacgc attcaatcta gtatttatgg tgaactttct aaaactaata gtattaatta
     7321 caattattta ctggctggtt gatatctact tgcgcgttaa aaacaaacta aaaaaataat
     7381 gactgtagat ttctgagcta ctttaaattc aaaataattc gggaaactga aattttgttg
     7441 ttgcaaaaca gaacgttagt ttgtataacg agactcaaaa gggcgttttt ttgtatgaaa
     7501 tttgattatt ccaaagaacc tgagcttgtg ttctaataac taaataacca taagcagtta
     7561 atttcaccaa ctagactaaa ctttctaaac caccttatca aataagatgg tttatacaga
     7621 aagtaactaa attaggagag caacaatcaa atacgactat caaccattga ctaacatgaa
     7681 aaaatactta aatttgattg ctgcaaaata aattttaaca ctttgtctat cgtttccatt
     7741 tttttatttt ataaatataa tcaaattaaa aaatctatat tgctgacaaa actttaagtc
     7801 gaatgaaata ttttattaac ccctataaag agagagtatg cctattgtat tgaccttctt
     7861 ttaaaatatg ttatatcata gacgcgaaac gaatttaatc gaacatgaaa aaccaaaaga
     7921 acctcctagt gtgaaaggca ggtctttttt aacgctgttg cgacacagcg ttaaaatcgg
     7981 aaaacgactg ggaatttatt catttttact tctaccattc atttctctcc attacttata
     8041 tataaatgtt tattatgtat tacttttatt tacttaccgc ttttattata ttaagttact
     8101 accttaaacc gttttatcta gtgtttttta tttacctacc actttaatgg tatttagcta
     8161 ccacctttta ctaatataga ttatttatat atcaacgttt agcatatctt acaccttatt
     8221 tacttactac ttttatttat tttagttact accttaagcc gttttatcta gtgtttttta
     8281 tttacctacc actttaatgg tatttagcta ccacctttta ctaatataga ttatttatat
     8341 atcaacgttt agcatatctt acaccttatt tacttactac ttttatttat tttagttact
     8401 accttaagcc gttttatcta gtgtttttta tttacctacc actttaatgg tatttagcta
     8461 ccacctttta ctaatataga ttatttatat atcaacgttt agcatatctt acaccttatt
     8521 tacttactac ttttatttat tttagttact accttaaacc gttttatcta gtgttttttt
     8581 atttacctac cactttaatg gtatttagct accacctttt actaatatag attgcttata
     8641 tatcaacatt taatatgtct tattctttat ttagttacta ctttaaatat attttaatac
     8701 acgtagaaaa agtgtacgga tcaaaatttg tacttctctt tatatacttc aaaacaatca
     8761 ttaaatacta aaaaaatacc taccacttta aataaatatg gtaggtattt ttttattttc
     8821 ttattttatc gttgattccc cattgtttct ttatatcagt aaatgtttct tgctttattt
     8881 cttcaggtga acgtatcaca ttattatcta tatctacatc aggaataata gggtgggtct
     8941 ctctaaataa atctaattca agagcaactg aatttagtaa atcattttta tcttcttgag
     9001 aaagatttaa tcgaatatat tcatttaaag cctttcgcaa taattcgtct tttttcatat
     9061 ttgtctcata cttttcaacc atgatgcttg ctgtagcatt agtaagggca gataatttta
     9121 cagactgcat tgctgcgtaa actttatcaa attttgttat tggagcacct tttctcttct
     9181 ttcgaatagg tgattcatga gctacagagc ttgttttctg gcttctcgag gtactattag
     9241 tcttagagat attacttata gttgtttcac ttgctttctc ttttgatcgt tgctttattt
     9301 tatctagtcc tttgttactg ctaccaaaga tacctaagtt tttaatttca gccattcttt
     9361 acacctaccc ttcataccaa tgttcatgtt cttctaattc gtccaaaata ttgataaata
     9421 attcatggca tttcttatcc cattgcttta attttccttt tttagttaat tcggtagtaa
     9481 taccggatcc atcgtatcgt ttaagtcgcg cattttttag cacaacgttt ttaattaagt
     9541 gctctccata gatttcttgt gcttgttgat acatttcttt atctacagaa tcaccacttt
     9601 ctaacataaa tggaacaaca ccagcaactt ttaaatttgt atcaaattca ttagtaaaga
     9661 agtccatgta ttccaaatac tgcatagcat tacgcataga tttaacctgt gtttgtaaca
     9721 caactatagc ccaatcagct atatacatag ctgatttact ataaatagaa attgatggag
     9781 gtacatctaa ataaatgaca tcaaaatcat taaataaagg ggatagtttt tcttccatgt
     9841 atttaaactt atcaattgaa ttaggaaatc tgtctttaat ccaatcatcg tataattcaa
     9901 acgacatatc cgcaggtaat atatatagat tgtcaataat cttataaata gcgtcatgtg
     9961 gatctacatt tgagataagc acatcagtaa ttgtatttgt atattcgatt agctcacctt
    10021 caccaccgcc ttcttcttta gaaaaattca tatgcgttaa gttcataaca tcagaagtat
    10081 tcccttgcat atctaagtca atgattaatg tctttaattc tttgaatttt gctgactcaa
    10141 atccaaa
//
