LOCUS       NZ_MUXL01000008        10604 bp    DNA     linear   CON 09-SEP-2024
DEFINITION  Bacillus spizizenii strain AS2 scaffold_8, whole genome shotgun
            sequence.
ACCESSION   NZ_MUXL01000008
VERSION     NZ_MUXL01000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus spizizenii
  ORGANISM  Bacillus spizizenii
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Al-Bahry,S.N., Al-Wahaibi,Y.M., Al-Sayegh,A., Elshafie,A.,
            Al-Bemani,A.S. and Joshi,S.J.
  TITLE     Whole Genome Sequence of Heavy-oil Degrading and Biosurfactant
            Producing Bacillus subtilis AS2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10604)
  AUTHORS   Al-Bahry,S.N., Al-Wahaibi,Y.M., Al-Sayegh,A., Elshafie,A.,
            Al-Bemani,A.S. and Joshi,S.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2017) Biology, Sultan Qaboos University, Al-Khod,
            Muscat 123, Oman
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_002155045.1
            Current Name                      :: Bacillus intestinalis
            Previous Name                     :: Bacillus subtilis
            Date Updated                      :: 2018-08-15
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. TYPE genome for current name
            A1 Genome (subject)               :: GCA_002027305.1
            A1 Name                           :: Bacillus intestinalis
            A1 ANI                            :: 99.23349%
            A1 Query Coverage                 :: 95.49%
            A1 Subject Coverage               :: 95.35%
            Analysis 2 (A2) :: Query vs. TYPE genome for previous name
            A2 Genome (subject)               :: GCA_000332645.1
            A2 Name                           :: Bacillus subtilis
            A2 ANI                            :: 94.5332%
            A2 Query Coverage                 :: 92.3%
            A2 Subject Coverage               :: 85.9%
            ##Taxonomic-Update-Statistics-END##
            ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 2.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002155045.1-RS_2024_09_09
            Annotation Date                   :: 09/09/2024 01:52:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,090
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 108
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 73 of 114
            Pseudo Genes (incomplete)         :: 53 of 114
            Pseudo Genes (internal stop)      :: 12 of 114
            Pseudo Genes (multiple problems)  :: 21 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:09:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10604
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MUXL01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..1837
                     /locus_tag="B0W20_RS14010"
                     /old_locus_tag="B0W20_14005"
     source          1..10604
                     /collection_date="2013-07"
                     /db_xref="taxon:96241"
                     /geo_loc_name="Oman: Heavy-Oil field"
                     /isolation_source="Heavy crude-oil contaminated soil
                     sample"
                     /mol_type="genomic DNA"
                     /organism="Bacillus spizizenii"
                     /strain="AS2"
                     /submitter_seqid="scaffold_8"
     region          1..10604
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..10604
                     /SMILES="NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10604
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1918:>10604](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1919..>10604
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             <3..1837
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (67-135). E-value: 1.1e-27. Score:
                     88.0. Matches aSDomain: nrpspksdomains_B0W20_RS14010_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (145-448). E-value:
                     1.8e-176. Score: 580.2. Matches aSDomain:
                     nrpspksdomains_B0W20_RS14010_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (453-607). E-value: 8e-56.
                     Score: 180.1. Matches aSDomain:
                     nrpspksdomains_B0W20_RS14010_TIGR01720.1"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388230.2"
                     /locus_tag="B0W20_RS14010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B0W20_14005"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_240508176.1"
                     /sec_met_domain="PP-binding (E-value: 3.8e-20, bitscore:
                     60.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.9e-98, bitscore:
                     317.6, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="YVTPETADIEALKSSLKETLPDYMIPAFWVTLNELPVTANGKVDR
                     KALPEPDIEAGSGEYKAPTTDMEELLAGIWQDVLGISEIGVSDNFFSLGGDSIKGIQMA
                     SRLNQHGWKLEMKDLFQHPTIEELTQYVERAEGKQADQGPVEGEVILTPIQRWFFEKNF
                     TNKHHWNQSVMLHAKKGFDPERVEKTLQALIEHHDALRMVYREGTKEVVQYNRGLEAAS
                     AQLEIIQIEGQAKDYEDRIEREAERLQSSIDLQEGGLLKAGLFQAEDGDHLLLAIHHLV
                     VDGVSWRILLEDFAAVYTQLEQSNEPVLPQKTHSFAEYAERLQDFANSKAFLKEKEYWR
                     QLEEQTVAAKLPKDRESGDQRMKHTKTIEFSLTAEETEQLTTKVHEAYHTEMNDILLTA
                     FGLAMKEWTGQDQVSVHLEGHGREEIIEDLTISRTVGWFTSMYPMVLDMKHTDDLGYQL
                     KQMKEDIRHVPNKGVGYGILRYLTAPEHKEDVAFSIQPDVSFNYLGQFDDMSDAGLFKR
                     SELPSGQSLSPETEKPNALDVVGYIENGKLTMSMAYHSLEFHEKTIKTFSDSFKAHLLR
                     IIEHCLSQDGTELTPSDLGDDDLTLDELDKLMEIF"
     aSDomain        206..409
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B0W20_RS14010_PCP.1"
                     /evalue="1.10E-27"
                     /label="B0W20_RS14010_PCP.1"
                     /locus_tag="B0W20_RS14010"
                     /protein_end="135"
                     /protein_start="67"
                     /score="88.0"
                     /tool="antismash"
                     /translation="EELLAGIWQDVLGISEIGVSDNFFSLGGDSIKGIQMASRLNQHGW
                     KLEMKDLFQHPTIEELTQYVERA"
     aSModule        206..1825
                     /domains="nrpspksdomains_B0W20_RS14010_PCP.1"
                     /domains="nrpspksdomains_B0W20_RS14010_Epimerization.1"
                     /domains="nrpspksdomains_B0W20_RS14010_TIGR01720.1"
                     /incomplete
                     /locus_tags="B0W20_RS14010"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        440..1348
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B0W20_RS14010_Epimerization.1"
                     /evalue="1.80E-176"
                     /label="B0W20_RS14010_Epimerization.1"
                     /locus_tag="B0W20_RS14010"
                     /protein_end="448"
                     /protein_start="145"
                     /score="580.2"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="EGEVILTPIQRWFFEKNFTNKHHWNQSVMLHAKKGFDPERVEKTL
                     QALIEHHDALRMVYREGTKEVVQYNRGLEAASAQLEIIQIEGQAKDYEDRIEREAERLQ
                     SSIDLQEGGLLKAGLFQAEDGDHLLLAIHHLVVDGVSWRILLEDFAAVYTQLEQSNEPV
                     LPQKTHSFAEYAERLQDFANSKAFLKEKEYWRQLEEQTVAAKLPKDRESGDQRMKHTKT
                     IEFSLTAEETEQLTTKVHEAYHTEMNDILLTAFGLAMKEWTGQDQVSVHLEGHGREEII
                     EDLTISRTVGWFTSMYPMVLDM"
     CDS_motif       455..484
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS14010_0001"
                     /evalue="3.80E-05"
                     /label="NRPS-E1"
                     /locus_tag="B0W20_RS14010"
                     /protein_end="160"
                     /protein_start="150"
                     /score="16.3"
                     /tool="antismash"
                     /translation="LTPIQRWFFE"
     CDS_motif       545..619
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS14010_0002"
                     /evalue="2.20E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="B0W20_RS14010"
                     /protein_end="205"
                     /protein_start="180"
                     /score="20.1"
                     /tool="antismash"
                     /translation="FDPERVEKTLQALIEHHDALRMVYR"
     CDS_motif       821..886
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS14010_0003"
                     /evalue="2.00E-12"
                     /label="NRPS-E2"
                     /locus_tag="B0W20_RS14010"
                     /protein_end="294"
                     /protein_start="272"
                     /score="39.1"
                     /tool="antismash"
                     /translation="LLLAIHHLVVDGVSWRILLEDF"
     CDS_motif       1295..1342
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS14010_0004"
                     /evalue="1.10E-08"
                     /label="NRPS-E5"
                     /locus_tag="B0W20_RS14010"
                     /protein_end="446"
                     /protein_start="430"
                     /score="27.0"
                     /tool="antismash"
                     /translation="ISRTVGWFTSMYPMVL"
     aSDomain        1364..1825
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B0W20_RS14010_TIGR01720.1"
                     /evalue="8.00E-56"
                     /label="B0W20_RS14010_TIGR01720.1"
                     /locus_tag="B0W20_RS14010"
                     /protein_end="607"
                     /protein_start="453"
                     /score="180.1"
                     /tool="antismash"
                     /translation="LGYQLKQMKEDIRHVPNKGVGYGILRYLTAPEHKEDVAFSIQPDV
                     SFNYLGQFDDMSDAGLFKRSELPSGQSLSPETEKPNALDVVGYIENGKLTMSMAYHSLE
                     FHEKTIKTFSDSFKAHLLRIIEHCLSQDGTELTPSDLGDDDLTLDELDKL"
     CDS_motif       1388..1447
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS14010_0005"
                     /evalue="1.10E-09"
                     /label="NRPS-E6"
                     /locus_tag="B0W20_RS14010"
                     /protein_end="481"
                     /protein_start="461"
                     /score="30.5"
                     /tool="antismash"
                     /translation="KEDIRHVPNKGVGYGILRYL"
     aSDomain        1919..2767
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B0W20_RS21555_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="3.50E-105"
                     /label="B0W20_RS21555_Condensation_DCL.1"
                     /locus_tag="B0W20_RS21555"
                     /protein_end="283"
                     /protein_start="0"
                     /score="343.3"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="MLDPHSSSYFTQLELGIHGAFDLEIFEKSVNELIRSYDILRTVFV
                     HQQLQKPRQVVLAERKTKVHYEDISHADQNHQKEHIERYKQDVQRQGFNLAKDMLFKVA
                     VFRLDADQLYLVWSNHHIMMDGWSMGVLMKSLFQNYEALRAGRTPANGQGKPYSDYIKW
                     LGKQDNEEAESYWSERLAGFEQPSVLPGRLPEKKDEYVNKEYSFTWDEKLVARIQQTAN
                     RHQVTGPNLFQAVWGIVLSKYNFTNDVVFGTVVSGRPSEINGIETMAGLFINTIPVRVK
                     VD"
     aSModule        1919..4888
                     /complete
                     /domains="nrpspksdomains_B0W20_RS21555_Condensation_DCL.1"
                     /domains="nrpspksdomains_B0W20_RS21555_AMP-binding.1"
                     /domains="nrpspksdomains_B0W20_RS21555_PCP.1"
                     /locus_tags="B0W20_RS21555"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     gene            1919..>4894
                     /gene="srfAB"
                     /locus_tag="B0W20_RS21555"
                     /pseudo=""
     CDS             1919..>4894
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Condensation_DCL (0-283). E-value:
                     3.5e-105. Score: 343.3. Matches aSDomain:
                     nrpspksdomains_B0W20_RS21555_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (447-841). E-value:
                     3.3e-111. Score: 363.7. Matches aSDomain:
                     nrpspksdomains_B0W20_RS21555_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (947-990). E-value: 3.2e-14. Score:
                     44.9. Matches aSDomain: nrpspksdomains_B0W20_RS21555_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="B0W20_RS21555"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 4.4e-84, bitscore:
                     270.4, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.4e-115, bitscore:
                     374.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.7e-09, bitscore:
                     25.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDPHSSSYFTQLELGIHGAFDLEIFEKSVNELIRSYDILRTVFV
                     HQQLQKPRQVVLAERKTKVHYEDISHADQNHQKEHIERYKQDVQRQGFNLAKDMLFKVA
                     VFRLDADQLYLVWSNHHIMMDGWSMGVLMKSLFQNYEALRAGRTPANGQGKPYSDYIKW
                     LGKQDNEEAESYWSERLAGFEQPSVLPGRLPEKKDEYVNKEYSFTWDEKLVARIQQTAN
                     RHQVTGPNLFQAVWGIVLSKYNFTNDVVFGTVVSGRPSEINGIETMAGLFINTIPVRVK
                     VDRDAAFADIFSAVQQHAVEAERYDYVPLYEIQKRSALDGNLLNHLVAFENYPLDQELE
                     NGSMEDRLGFSIKVESAFEQTSFDFNLIVYPGKTWIVKIKYNGAAFDSAFIERAAEHLT
                     RMMEAAVDQPDAFVREYGLVGDEEQRQIVEVFNQTKAEFPEGMAVHQVFEEQVQRTPAS
                     TAVVYEGAELTYRQLNTAANRLARKLVEQGLQKGETAAIMNDRSAETVVGMLAVLKAGA
                     AYVPLDPALPVDRLRFMAEDSSIRMVLAGKSYTEQAYQLQVPVLTLDIGFEECEAADNL
                     NLPSAPSDLGYIMYTSGSTGKPKGVMIEHKSILRLVKNAGYVPVHEEDRMAQTGAVSFD
                     AGTFEVFGALLNGAALYPVKKETLLDAKQFAAFLREQRITTMWLTSPLFNQLASKDAGM
                     FGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNGYGPTENTTFSTSFLIDREFSGSIPI
                     GKPIGNSTAYIMDEQQRLQPIGAPGELCVGGNGVARGYVNLPELTEKQFLEDPFRPGER
                     IYRTGDLARWLPDGNIEFLGRIDNQVKVRGFRIELGEIETKLNMAEHVTEAAVIIRKNK
                     ADENEICAYFTADREVSVSWLRKTLSQSLPDYMVPAHLIQMDSLPLTPNGKINKKELPA
                     PQSEAVQPEYAAPETESEKKLAEIWEGILGVKAGVTDNFFMIGGHSLKAMMMTAKIQEH
                     FHK"
     CDS_motif       1940..2056
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21555_0001"
                     /evalue="2.70E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="B0W20_RS21555"
                     /protein_end="46"
                     /protein_start="7"
                     /score="45.4"
                     /tool="antismash"
                     /translation="SYFTQLELGIHGAFDLEIFEKSVNELIRSYDILRTVFVH"
     CDS_motif       2273..2338
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21555_0002"
                     /evalue="1.10E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="B0W20_RS21555"
                     /protein_end="140"
                     /protein_start="118"
                     /score="37.1"
                     /tool="antismash"
                     /translation="NHHIMMDGWSMGVLMKSLFQNY"
     CDS_motif       2384..2422
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21555_0003"
                     /evalue="1.10E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="B0W20_RS21555"
                     /protein_end="168"
                     /protein_start="155"
                     /score="11.5"
                     /tool="antismash"
                     /translation="PYSDYIKWLGKQD"
     CDS_motif       2660..2755
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21555_0004"
                     /evalue="7.20E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="B0W20_RS21555"
                     /protein_end="279"
                     /protein_start="247"
                     /score="63.4"
                     /tool="antismash"
                     /translation="DVVFGTVVSGRPSEINGIETMAGLFINTIPVR"
     CDS_motif       2804..2920
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21555_0005"
                     /evalue="1.60E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="B0W20_RS21555"
                     /protein_end="334"
                     /protein_start="295"
                     /score="55.9"
                     /tool="antismash"
                     /translation="QQHAVEAERYDYVPLYEIQKRSALDGNLLNHLVAFENYP"
     aSDomain        3260..4441
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B0W20_RS21555_AMP-binding.1"
                     /evalue="3.30E-111"
                     /label="B0W20_RS21555_AMP-binding.1"
                     /locus_tag="B0W20_RS21555"
                     /protein_end="841"
                     /protein_start="447"
                     /score="363.7"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEEQVQRTPASTAVVYEGAELTYRQLNTAANRLARKLVEQGLQKG
                     ETAAIMNDRSAETVVGMLAVLKAGAAYVPLDPALPVDRLRFMAEDSSIRMVLAGKSYTE
                     QAYQLQVPVLTLDIGFEECEAADNLNLPSAPSDLGYIMYTSGSTGKPKGVMIEHKSILR
                     LVKNAGYVPVHEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFL
                     REQRITTMWLTSPLFNQLASKDAGMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNG
                     YGPTENTTFSTSFLIDREFSGSIPIGKPIGNSTAYIMDEQQRLQPIGAPGELCVGGNGV
                     ARGYVNLPELTEKQFLEDPFRPGERIYRTGDLARWLPDGNIEFLGRIDNQVKVR"
     CDS_motif       3446..3487
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21555_0006"
                     /evalue="1.50E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="B0W20_RS21555"
                     /protein_end="523"
                     /protein_start="509"
                     /score="21.1"
                     /tool="antismash"
                     /translation="LAVLKAGAAYVPLD"
     CDS_motif       3677..3733
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21555_0007"
                     /evalue="1.20E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="B0W20_RS21555"
                     /protein_end="605"
                     /protein_start="586"
                     /score="33.2"
                     /tool="antismash"
                     /translation="YIMYTSGSTGKPKGVMIEH"
     CDS_motif       4235..4324
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21555_0008"
                     /evalue="6.30E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="B0W20_RS21555"
                     /protein_end="802"
                     /protein_start="772"
                     /score="47.3"
                     /tool="antismash"
                     /translation="PIGAPGELCVGGNGVARGYVNLPELTEKQF"
     CDS_motif       4406..4471
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21555_0009"
                     /evalue="1.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="B0W20_RS21555"
                     /protein_end="851"
                     /protein_start="829"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRIDNQVKVRGFRIELGEIE"
     aSDomain        4760..4888
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B0W20_RS21555_PCP.1"
                     /evalue="3.20E-14"
                     /label="B0W20_RS21555_PCP.1"
                     /locus_tag="B0W20_RS21555"
                     /protein_end="990"
                     /protein_start="947"
                     /score="44.9"
                     /tool="antismash"
                     /translation="EKKLAEIWEGILGVKAGVTDNFFMIGGHSLKAMMMTAKIQEHF"
     gene            4986..5126
                     /gene="comS"
                     /locus_tag="B0W20_RS21560"
     CDS             4986..5126
                     /codon_start=1
                     /gene="comS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388232.1"
                     /locus_tag="B0W20_RS21560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="competence protein ComS"
                     /protein_id="WP_010886404.1"
                     /transl_table=11
                     /translation="MNRSGKHLISSIILYPRPSGECISSISLDKQTQATTSPLYFCWRE
                     K"
     gene            <5072..>8014
                     /gene="srfAB"
                     /locus_tag="B0W20_RS21565"
                     /pseudo=""
     CDS             5072..>8014
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (2-264). E-value:
                     5.2e-95. Score: 309.8. Matches aSDomain:
                     nrpspksdomains_B0W20_RS21565_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (427-830). E-value:
                     1.1e-113. Score: 371.9. Matches aSDomain:
                     nrpspksdomains_B0W20_RS21565_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (936-980). E-value: 5.9e-17. Score:
                     53.6. Matches aSDomain: nrpspksdomains_B0W20_RS21565_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="B0W20_RS21565"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 7.4e-86, bitscore:
                     276.3, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 9.6e-125, bitscore:
                     404.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.1e-10, bitscore:
                     30.4, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MANTSYNVPAVLLLEGEVDKDRLEKAIQQLINRHEILRTSFDMID
                     GEVIQTVHKNISFQLEAAKGREEDAEEMIKAFIQPFELNRAPLVRSKLVQLEEERHLLL
                     IDMHHIITDGSSTGILIGDLAKMYQGADLEQPQIHYKDYAVWHKEHADHQKDEAYWLDT
                     FKGELPILDLPTDFERPAERSFAGERVMFGLDKQMSTQIKSLLAETDTTMYMFLLAAFN
                     VLLSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTAPAGDKTFAQFLEEVKTA
                     SLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNMQNMEIPSLRLGDLKISSYSMLH
                     HVAKFDLSLEAVEREEDIGLSFDYATALFKDETIRRWSSHFVNIIKAAAANPNVRLSDV
                     DLLSPAETAALLEERHMTQITEATFAALFEEQAQQTPDHSAVRSGGNLLTYRELDEQAN
                     QLAHHLRAQGAGSEDIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLED
                     SGARIAVVNERNMTAIGQYEGTIINLDDAKWRNESKERPSPISGSRNLAYVIYTSGTTG
                     KPKGVQIEQRNLTNYVSWFSEEAGLTETDKTVLLSSYAFDLGYTSMFPVLLAGGELHIV
                     PKETYTAPDEMGRYIKEHGITYIKLTPSLFHTVVNTASFLKELNFESLRLIVLGGEKII
                     PNDVLAFRKMYGHTEFINHYGPTEATIGAIAGRVNLSEPDAFAQRPTIGQPIANAGALV
                     LNEALKLVPPGASGQLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRL
                     SNGTLEFIGRADDQVKIRGYRIEPKEIETVMLSLSGIQEAVVLAVSEGGLQELCAYYTA
                     DQAIEKAVLRNQLSQTLPSHMIPAFFVQVDAIPLTANGKTDRNALPKPNTEQSGRRTSA
                     APETELEESLCRIWKKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAELE"
     aSDomain        5078..5863
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B0W20_RS21565_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="5.20E-95"
                     /label="B0W20_RS21565_Condensation_LCL.1"
                     /locus_tag="B0W20_RS21565"
                     /protein_end="264"
                     /protein_start="2"
                     /score="309.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIITDG"
                     /tool="antismash"
                     /translation="NTSYNVPAVLLLEGEVDKDRLEKAIQQLINRHEILRTSFDMIDGE
                     VIQTVHKNISFQLEAAKGREEDAEEMIKAFIQPFELNRAPLVRSKLVQLEEERHLLLID
                     MHHIITDGSSTGILIGDLAKMYQGADLEQPQIHYKDYAVWHKEHADHQKDEAYWLDTFK
                     GELPILDLPTDFERPAERSFAGERVMFGLDKQMSTQIKSLLAETDTTMYMFLLAAFNVL
                     LSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTA"
     aSModule        5078..8011
                     /complete
                     /domains="nrpspksdomains_B0W20_RS21565_Condensation_LCL.1"
                     /domains="nrpspksdomains_B0W20_RS21565_AMP-binding.1"
                     /domains="nrpspksdomains_B0W20_RS21565_PCP.1"
                     /locus_tags="B0W20_RS21565"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       5081..5194
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21565_0001"
                     /evalue="4.30E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="B0W20_RS21565"
                     /protein_end="41"
                     /protein_start="3"
                     /score="57.7"
                     /tool="antismash"
                     /translation="TSYNVPAVLLLEGEVDKDRLEKAIQQLINRHEILRTSF"
     CDS_motif       5390..5455
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21565_0002"
                     /evalue="1.40E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="B0W20_RS21565"
                     /protein_end="128"
                     /protein_start="106"
                     /score="27.2"
                     /tool="antismash"
                     /translation="MHHIITDGSSTGILIGDLAKMY"
     CDS_motif       5486..5518
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21565_0003"
                     /evalue="1.30E-03"
                     /label="C4_LCL_164-176"
                     /locus_tag="B0W20_RS21565"
                     /protein_end="149"
                     /protein_start="138"
                     /score="11.5"
                     /tool="antismash"
                     /translation="HYKDYAVWHKE"
     CDS_motif       5768..5857
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21565_0004"
                     /evalue="9.00E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="B0W20_RS21565"
                     /protein_end="262"
                     /protein_start="232"
                     /score="50.2"
                     /tool="antismash"
                     /translation="DIIVGSPTAGRTHPDLQGVPGMFVNTVALR"
     CDS_motif       5912..6025
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21565_0005"
                     /evalue="2.40E-16"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="B0W20_RS21565"
                     /protein_end="318"
                     /protein_start="280"
                     /score="52.1"
                     /tool="antismash"
                     /translation="ASLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNM"
     aSDomain        6353..7561
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B0W20_RS21565_AMP-binding.1"
                     /evalue="1.10E-113"
                     /label="B0W20_RS21565_AMP-binding.1"
                     /locus_tag="B0W20_RS21565"
                     /protein_end="830"
                     /protein_start="427"
                     /score="371.9"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FEEQAQQTPDHSAVRSGGNLLTYRELDEQANQLAHHLRAQGAGSE
                     DIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLEDSGARIAVVNERNMT
                     AIGQYEGTIINLDDAKWRNESKERPSPISGSRNLAYVIYTSGTTGKPKGVQIEQRNLTN
                     YVSWFSEEAGLTETDKTVLLSSYAFDLGYTSMFPVLLAGGELHIVPKETYTAPDEMGRY
                     IKEHGITYIKLTPSLFHTVVNTASFLKELNFESLRLIVLGGEKIIPNDVLAFRKMYGHT
                     EFINHYGPTEATIGAIAGRVNLSEPDAFAQRPTIGQPIANAGALVLNEALKLVPPGASG
                     QLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRLSNGTLEFIGRADDQ
                     VKIR"
     CDS_motif       6539..6580
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21565_0006"
                     /evalue="3.30E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="B0W20_RS21565"
                     /protein_end="503"
                     /protein_start="489"
                     /score="17.1"
                     /tool="antismash"
                     /translation="LGVMKAGAAFLPID"
     CDS_motif       6767..6823
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21565_0007"
                     /evalue="8.10E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="B0W20_RS21565"
                     /protein_end="584"
                     /protein_start="565"
                     /score="30.6"
                     /tool="antismash"
                     /translation="AYVIYTSGTTGKPKGVQIE"
     CDS_motif       7355..7444
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21565_0008"
                     /evalue="4.20E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="B0W20_RS21565"
                     /protein_end="791"
                     /protein_start="761"
                     /score="51.0"
                     /tool="antismash"
                     /translation="PPGASGQLYITGQGLARGYLNRPQLTAERF"
     CDS_motif       7526..7591
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21565_0009"
                     /evalue="3.30E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="B0W20_RS21565"
                     /protein_end="840"
                     /protein_start="818"
                     /score="38.3"
                     /tool="antismash"
                     /translation="FIGRADDQVKIRGYRIEPKEIE"
     aSDomain        7880..8011
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B0W20_RS21565_PCP.1"
                     /evalue="5.90E-17"
                     /label="B0W20_RS21565_PCP.1"
                     /locus_tag="B0W20_RS21565"
                     /protein_end="980"
                     /protein_start="936"
                     /score="53.6"
                     /tool="antismash"
                     /translation="EESLCRIWKKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAEL"
     aSDomain        8165..8992
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B0W20_RS21570_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="8.70E-100"
                     /label="B0W20_RS21570_Condensation_LCL.1"
                     /locus_tag="B0W20_RS21570"
                     /protein_end="276"
                     /protein_start="0"
                     /score="325.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="MYVLNQLDRQTISYNMPSVLLMEGELDISRLRDSLNQLVNRHESL
                     RTSFMEANGEPVQRIIEKAAIDLHVFEAKEDEAEQKIKDFIRPFDLNGAPLIRAALLRI
                     EAKKHLLLLDMHHIIADGVSRGIFVKELALLYKGEQLPEPALHYKDFAVWQNEAEQKER
                     MKEHEAYWLSVLSGELPELDLPLDYARPPVQSFKGDTVRFRTGSETAKAVEKLLAETGT
                     TLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRMNADVQDMPGMFVNTLALRMEA"
     aSModule        8165..10597
                     /domains="nrpspksdomains_B0W20_RS21570_Condensation_LCL.1"
                     /domains="nrpspksdomains_B0W20_RS21570_AMP-binding.1"
                     /incomplete
                     /locus_tags="B0W20_RS21570"
                     /tool="antismash"
                     /type="nrps"
     gene            8165..>10604
                     /locus_tag="B0W20_RS21570"
     CDS             8165..>10604
                     /NRPS_PKS="Domain: Condensation_LCL (0-276). E-value:
                     8.7e-100. Score: 325.5. Matches aSDomain:
                     nrpspksdomains_B0W20_RS21570_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (441-811). E-value: 1.1e-97.
                     Score: 319.2. Matches aSDomain:
                     nrpspksdomains_B0W20_RS21570_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="B0W20_RS21570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_240508177.1"
                     /sec_met_domain="Condensation (E-value: 3.6e-92, bitscore:
                     297.0, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 6.1e-96, bitscore:
                     309.8, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYVLNQLDRQTISYNMPSVLLMEGELDISRLRDSLNQLVNRHESL
                     RTSFMEANGEPVQRIIEKAAIDLHVFEAKEDEAEQKIKDFIRPFDLNGAPLIRAALLRI
                     EAKKHLLLLDMHHIIADGVSRGIFVKELALLYKGEQLPEPALHYKDFAVWQNEAEQKER
                     MKEHEAYWLSVLSGELPELDLPLDYARPPVQSFKGDTVRFRTGSETAKAVEKLLAETGT
                     TLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRMNADVQDMPGMFVNTLALRMEAEEQQT
                     FAELLEQAKQTNLSALEHQEYPFEDLVNQLDLPRDMSRNPLFNVMVTTENPDKEQLTLE
                     NLSISPYESHQGTSKFDLTLGGFTDENGIGLQLEYATDLFSKETAEKWSEYVLRLLKAV
                     AENPNQPLSSLSLVTETEKQTLLEAWTGKTLPVPTDKTVHQLFEETAQHHKDRPAVTYK
                     GQSWTYGELNAKANRLARILIDCGISPDERVGVLTKPSLEMSAAVLGVLKAGAAFVPID
                     PDYPDQRIDYILQDSGAKLLLKQEGIAVPDSYTGEIILLDGNRTILSLPLDENDEENPE
                     TDTNAENLAYMIYTSGTTGQPKGVMVEHHALVNLCFWHHDAFSMTAEDRSAKYAGFGFD
                     ASIWEMFPTWTIGAELHVIDEAIRLDIVRLNDYFETNGVTITFLPTQLAEQFMELENTS
                     LRVLLTGGDKLKRAVKQPYTLVNNYGPTENTVVATSTEINPEEGSLSIGQAIANTRVYI
                     LGEGNQVQPEGVAGELCVAGRGLARGYLNKEDETAKRFVADPFVPGERMYRTGDLVKWV
                     N"
     CDS_motif       8201..8311
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21570_0001"
                     /evalue="4.80E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="B0W20_RS21570"
                     /protein_end="49"
                     /protein_start="12"
                     /score="54.3"
                     /tool="antismash"
                     /translation="SYNMPSVLLMEGELDISRLRDSLNQLVNRHESLRTSF"
     CDS_motif       8507..8572
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21570_0002"
                     /evalue="1.40E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="B0W20_RS21570"
                     /protein_end="136"
                     /protein_start="114"
                     /score="30.4"
                     /tool="antismash"
                     /translation="MHHIIADGVSRGIFVKELALLY"
     CDS_motif       8603..8635
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21570_0003"
                     /evalue="9.40E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="B0W20_RS21570"
                     /protein_end="157"
                     /protein_start="146"
                     /score="15.0"
                     /tool="antismash"
                     /translation="HYKDFAVWQNE"
     CDS_motif       8894..8983
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21570_0004"
                     /evalue="6.40E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="B0W20_RS21570"
                     /protein_end="273"
                     /protein_start="243"
                     /score="44.2"
                     /tool="antismash"
                     /translation="DIVIGSVTAGRMNADVQDMPGMFVNTLALR"
     CDS_motif       9032..9145
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21570_0005"
                     /evalue="4.50E-18"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="B0W20_RS21570"
                     /protein_end="327"
                     /protein_start="289"
                     /score="57.6"
                     /tool="antismash"
                     /translation="KQTNLSALEHQEYPFEDLVNQLDLPRDMSRNPLFNVMV"
     aSDomain        9488..10597
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B0W20_RS21570_AMP-binding.1"
                     /evalue="1.10E-97"
                     /label="B0W20_RS21570_AMP-binding.1"
                     /locus_tag="B0W20_RS21570"
                     /protein_end="811"
                     /protein_start="441"
                     /score="319.2"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="FEETAQHHKDRPAVTYKGQSWTYGELNAKANRLARILIDCGISPD
                     ERVGVLTKPSLEMSAAVLGVLKAGAAFVPIDPDYPDQRIDYILQDSGAKLLLKQEGIAV
                     PDSYTGEIILLDGNRTILSLPLDENDEENPETDTNAENLAYMIYTSGTTGQPKGVMVEH
                     HALVNLCFWHHDAFSMTAEDRSAKYAGFGFDASIWEMFPTWTIGAELHVIDEAIRLDIV
                     RLNDYFETNGVTITFLPTQLAEQFMELENTSLRVLLTGGDKLKRAVKQPYTLVNNYGPT
                     ENTVVATSTEINPEEGSLSIGQAIANTRVYILGEGNQVQPEGVAGELCVAGRGLARGYL
                     NKEDETAKRFVADPFVPGERMYRTGDLVKW"
     CDS_motif       9674..9715
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21570_0006"
                     /evalue="7.50E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="B0W20_RS21570"
                     /protein_end="517"
                     /protein_start="503"
                     /score="19.0"
                     /tool="antismash"
                     /translation="LGVLKAGAAFVPID"
     CDS_motif       9917..9976
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21570_0007"
                     /evalue="5.70E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="B0W20_RS21570"
                     /protein_end="604"
                     /protein_start="584"
                     /score="34.1"
                     /tool="antismash"
                     /translation="AYMIYTSGTTGQPKGVMVEH"
     CDS_motif       10448..10537
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B0W20_RS21570_0008"
                     /evalue="3.40E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="B0W20_RS21570"
                     /protein_end="791"
                     /protein_start="761"
                     /score="48.1"
                     /tool="antismash"
                     /translation="PEGVAGELCVAGRGLARGYLNKEDETAKRF"
ORIGIN
        1 gtacgtcaca ccggaaacag ctgacataga agcattaaag tcttcgttga aagaaaccct
       61 gccggactac atgattccgg cgttctgggt gacgctgaac gagcttccag ttacggcaaa
      121 cggaaaggtt gaccgcaaag ccttgcctga gccggatatc gaagcgggaa gcggagaata
      181 caaggcgccg acaacggata tggaagagct gcttgccggc atctggcagg acgtgctcgg
      241 catttcggag atcggtgtca gcgacaattt cttctcgctt ggcggagatt ccatcaaagg
      301 aatccaaatg gcgagccgct tgaaccagca cggctggaag ctggagatga aagatctctt
      361 ccagcacccg acgatcgagg agctgaccca atacgtggag cgtgccgaag gcaaacaggc
      421 agaccaaggc ccggtggagg gcgaagtcat tctgacgccg attcagcgtt ggttctttga
      481 aaagaacttc acgaacaagc accactggaa ccaatcagtg atgcttcatg cgaagaaggg
      541 ctttgatcct gaacgggtgg agaaaacgtt gcaggcgctg atcgagcacc atgacgcgct
      601 ccgcatggtt tatcgtgagg gaacgaaaga ggtcgttcaa tacaacagag gacttgaagc
      661 tgcttcagct caattggaga tcatccaaat cgagggccaa gcgaaagatt atgaagaccg
      721 catagagaga gaagcggaac ggctgcagag cagcatcgac ttgcaggaag gcggcttgtt
      781 aaaagcaggc ttgttccaag cggaagacgg agaccatttg cttcttgcca ttcaccactt
      841 agtggttgac ggcgtgtcgt ggcggatttt actggaggat ttcgccgcgg tatatacaca
      901 gcttgagcaa agcaatgaac cggttctccc acagaaaaca cattcatttg cggagtatgc
      961 agagagatta caagacttcg cgaattccaa agcctttttg aaagaaaaag agtattggag
     1021 acagcttgaa gaacaaactg tcgcggcaaa gcttccgaag gatcgcgaat ctggtgatca
     1081 gcgaatgaaa catacaaaga caatcgaatt ttcgctgact gctgaagaaa cagaacagct
     1141 caccacaaag gtgcatgagg catatcacac agaaatgaat gatattttac tgacagcatt
     1201 cggattggcg atgaaggagt ggacaggtca ggatcaagta agtgttcatt tagaggggca
     1261 tggacgtgaa gaaatcatag aagacctgac catttcccgg acagtcggct ggtttacgag
     1321 tatgtacccg atggtgctcg atatgaagca tacggatgat ctgggctacc agctgaagca
     1381 aatgaaagaa gatatcagac atgtaccgaa taagggagtc gggtacggca ttctccgcta
     1441 tttgacggcg ccggaacata aagaagatgt ggcgttctcg attcagccgg atgtcagctt
     1501 taactactta ggacagttcg atgacatgtc ggatgcaggc ttgttcaaga gatcggagct
     1561 gccgtcagga cagtcgttaa gcccggaaac agaaaaaccg aatgcgctcg atgttgtcgg
     1621 atacattgaa aacggaaaac tgacgatgtc aatggcctat cattctcttg aatttcatga
     1681 aaaaacgata aagacattca gcgacagctt taaagcccat cttctcagaa tcattgaaca
     1741 ttgcctatct caagatggaa cggaactgac gccgagtgac cttggcgacg acgatttgac
     1801 gctggatgaa ctggataaat taatggaaat tttctaatag aggtggcata tgagcaaaaa
     1861 atcgattcaa aaggtgtacg cactgacacc gatgcaggag ggaatgctgt atcatgcgat
     1921 gcttgatccg cattcatcct cgtattttac acaattagag cttggaattc acggtgcttt
     1981 tgatcttgaa atctttgaga aaagcgtcaa tgaactgatt cggtcatacg atattctccg
     2041 tacggtattt gtgcatcagc agctgcaaaa accgcgtcag gtcgtgttag cggaacgcaa
     2101 aacaaaggtg cactatgaag atattagtca tgcggaccaa aaccaccaga aagagcatat
     2161 tgagcgttat aaacaagacg tgcagcgcca aggttttaac ctggcaaaag acatgctgtt
     2221 caaggtggcg gttttccgtc ttgatgcaga tcagctgtat cttgtctgga gtaatcatca
     2281 tattatgatg gacggctgga gcatgggcgt actcatgaaa agcctgttcc aaaattatga
     2341 agcgctcaga gcaggccgaa caccggcaaa cggtcaggga aagccttatt ccgactacat
     2401 caagtggctt ggaaaacagg acaatgaaga agcggagagc tactggagcg aacgcttggc
     2461 gggttttgaa cagccgagcg tgctcccggg ccgcctgcct gagaaaaaag acgaatacgt
     2521 taacaaagaa tattcattta catgggatga aaaactggtt gcccgcattc agcaaaccgc
     2581 aaaccgccat caagtgacag gacctaacct gtttcaggcc gtttggggca ttgttctcag
     2641 caaatacaac tttacgaatg acgttgtctt cggcacggtc gtatcaggcc ggccatctga
     2701 aatcaacggg attgaaacga tggcaggact gtttatcaac accatcccgg ttcgggtaaa
     2761 agttgatcgt gatgctgctt tcgctgatat tttctcagct gttcagcagc atgcagttga
     2821 ggcggagcgc tacgattacg taccgctcta tgagattcaa aaacgctcag ctcttgatgg
     2881 caatctcttg aaccatctgg tcgcgtttga aaattatccg cttgaccaag agcttgaaaa
     2941 cggcagcatg gaagaccgcc tcggattttc tatcaaggta gaaagcgcat ttgagcaaac
     3001 gagcttcgat ttcaatctga ttgtgtatcc aggcaaaacg tggattgtca aaatcaaata
     3061 taacggtgcc gcctttgatt ctgcttttat cgaacgggca gcggagcacc ttacccgcat
     3121 gatggaagcg gctgtcgatc agccggacgc ttttgtccgt gagtacgggc ttgtgggaga
     3181 cgaagagcag cggcaaattg tcgaggtgtt taaccagacg aaagccgaat ttccagaagg
     3241 aatggccgtt catcaagtgt ttgaagagca agtgcagcga acaccggcaa gcactgccgt
     3301 cgtatatgaa ggagccgagc tgacataccg tcagctgaat acagcggcaa accgtctggc
     3361 gagaaagctt gtcgaacaag gtcttcaaaa aggggaaaca gccgcgatta tgaacgaccg
     3421 ctcagcagaa accgttgtcg gcatgctggc tgtgttaaaa gcaggcgccg cctatgtgcc
     3481 gcttgatccc gcacttcctg tggatcgtct tcgtttcatg gctgaggaca gctcgattcg
     3541 gatggtattg gccggaaagt cttatacaga gcaggcgtat cagctgcaag tgccggttct
     3601 tacactggac atcggctttg aagagtgcga agctgctgac aatctcaatc tgccttccgc
     3661 accgtctgat ttgggataca tcatgtatac atctggttcg acgggcaaac cgaaaggcgt
     3721 catgattgaa cataaaagta ttcttcgtct cgtcaaaaat gccggttacg ttcctgttca
     3781 tgaggaggac cgcatggcgc aaacgggagc tgtcagcttt gatgccggca cgtttgaggt
     3841 attcggcgca ctgctgaatg gcgccgcgct ttatccggtc aaaaaagaga cgttgcttga
     3901 tgcgaaacaa tttgcggcat tcctgcgtga gcagagaatc acgaccatgt ggctgacatc
     3961 acctttattc aaccagcttg cttcaaagga tgccggcatg ttcggcacac tgcgccattt
     4021 aatcatcggc ggagatgcgc tcgtgccgca tattgtcagc aaagtgaagc aggcatcgcc
     4081 gtcgctttcg ttgtggaacg gatacggccc gacagaaaac acgacatttt caacaagttt
     4141 tctgatcgac cgtgagttta gcggctctat tccaatcggg aagcctatcg gaaattcgac
     4201 tgcctacatc atggatgagc agcagcgttt gcagccaatt ggcgcgcccg gcgagctgtg
     4261 tgtcggagga aacggggtag cgcgaggtta tgtcaatctt cctgagctga cggagaagca
     4321 atttcttgaa gatccgttca gaccgggtga aaggatttat cgcactggcg acttggcgag
     4381 atggctgccg gacggcaata tcgaattttt aggaagaatc gacaatcaag tgaaggtgcg
     4441 cggcttccga attgagcttg gcgaaattga aacaaaactg aacatggcag agcatgtgac
     4501 agaggctgca gtgatcatcc gcaagaacaa agcagatgaa aatgaaattt gcgcgtactt
     4561 tacggcggac cgtgaagtgt ctgtgagctg gctgagaaaa acgctgtccc agtctttgcc
     4621 tgactatatg gttcctgccc atttgattca gatggacagc ctgccgctca cgccaaacgg
     4681 aaaaatcaac aaaaaagaac tgcctgcgcc tcaatcagaa gccgtgcagc cggagtacgc
     4741 agcaccagaa acagagagtg aaaagaaatt agcggagatc tgggaaggga tactcggcgt
     4801 caaagcaggc gttaccgata acttctttat gatcggcggt cattctttga aagcgatgat
     4861 gatgacggcg aaaatccagg agcattttca taaggaagta ccgattaaag tgctctttga
     4921 aaagccgacc attcaagaac tggcgctgta tttggacgag actgaaagca aggaggagca
     4981 gacgtttgaa ccgatcaggc aagcacctta tcagcagcat tatcctgtat ccccggccca
     5041 gcggagaatg tatatcctca atcagcttgg acaagcaaac acaagctaca acgtccccgc
     5101 tgtacttttg ctggagggag aagtagataa agaccggctt gaaaaagcga ttcagcaatt
     5161 aatcaaccgg cacgaaatcc ttcgtacatc gtttgacatg atcgacggag aagttataca
     5221 aaccgttcat aaaaacatat cgttccagct ggaggctgcc aagggacggg aagaagacgc
     5281 ggaggaaatg atcaaagcgt tcattcagcc gtttgaatta aaccgcgcgc cgctcgtccg
     5341 ttcgaagctt gtccagcttg aagaagaacg ccacctgctg ctgattgata tgcaccatat
     5401 cattactgac ggcagttcaa caggcattct gatcggtgat ctcgccaaaa tgtatcaagg
     5461 cgctgatctt gagcagccgc aaattcacta taaagattac gctgtttggc ataaagaaca
     5521 tgctgatcat caaaaggatg aggcatactg gctcgatacc tttaaagggg agctgccgat
     5581 tcttgatctt ccgacggatt tcgagcggcc tgctgaacgg agctttgctg gagagcgcgt
     5641 gatgtttggg cttgataaac agatgtcaac tcaaatcaag tcactcttgg cagaaacgga
     5701 tacgacaatg tatatgtttt tgctggcggc gttcaatgta ctcctttcta agtacgcgtc
     5761 acaggacgat atcattgtcg gctcgccgac tgcgggaaga acacatcctg atctgcaagg
     5821 tgtgccgggg atgtttgtca acacggtcgc actaagaacg gcgcctgcgg gagataaaac
     5881 ctttgcgcaa ttccttgaag aagtcaaaac agcaagcctt caagcattcg agcaccagag
     5941 ctatcccctt gaggagctga ttgaaaagct tccgcttaca agggatacaa gccgaagtcc
     6001 gctgttcagc gtgatgttca atatgcagaa catggagatt ccatcattga gattaggaga
     6061 tttgaagatt tcttcatact ccatgcttca tcacgtggct aaatttgatc tctccttgga
     6121 agcggtcgag cgagaagagg atatcggcct gagctttgac tatgcgactg ccctgtttaa
     6181 ggacgagacg attcggcgct ggagcagcca ctttgtcaat atcatcaaag cggccgcagc
     6241 taatccgaac gttcggctgt ctgatgtaga cctgctttcc ccagcagaaa cggctgcttt
     6301 gcttgaagaa agacatatga ctcaaattac tgaagcaacc tttgcagctc ttttcgaaga
     6361 acaggcccag caaacacctg atcattctgc ggtgaggtct ggggggaatc tgctgaccta
     6421 tcgcgagctt gatgaacagg cgaaccagct ggcgcaccat cttcgtgcgc aaggggcagg
     6481 aagtgaagac atcgtcgcaa ttgtcatgga tcggtcagct gaggttatgg tatcgattct
     6541 tggtgtcatg aaagcggggg cagctttcct gccgattgat cctgatacgc ctgaggaacg
     6601 aatccgctat tcattagagg acagcggtgc gagaattgcg gtcgtgaacg aaagaaacat
     6661 gacggccatt gggcaatatg aagggacaat catcaacctt gatgatgcaa aatggagaaa
     6721 tgagagtaag gagcgcccct ctccgatctc cggatctcgc aatcttgcat acgtcattta
     6781 tacgtccggt acgaccggga agccgaaggg cgtgcagatc gagcagcgca atctgacaaa
     6841 ctatgtttct tggtttagtg aagaggcggg cctgacggaa accgataaaa ctgtattgct
     6901 ttcgtcttac gcattcgacc taggctatac gagcatgttc cctgtacttc tggcaggggg
     6961 cgagctccac atcgtgccga aggaaaccta tacggcgccg gatgaaatgg ggcgttatat
     7021 caaggaacac gggatcacat atatcaaact gacaccgtct ctgttccata cggtggtgaa
     7081 cacggccagc tttttaaaag agctaaactt tgaatccttg cgcttgatcg tcttgggagg
     7141 agaaaaaatc atcccgaatg atgttctggc cttccgcaag atgtatggac ataccgaatt
     7201 tatcaatcat tacggcccga ctgaagcaac aatcggcgcc atcgccggac gggttaatct
     7261 gtctgaaccg gatgcattcg cgcaacgccc gacaatcggg cagccgattg cgaatgccgg
     7321 tgcgcttgtc ttaaatgagg cgttaaagct tgtgcctccg ggagcgagcg gccagctcta
     7381 tatcacggga caggggcttg ctagaggata tctcaacagg cctcagctga cagccgagag
     7441 atttgttgaa aatccatatt cgccgggcag tctcatgtac aaaaccggag atgtcgtacg
     7501 gagactttct aacggtacgc ttgaatttat cggccgggct gatgatcagg tgaaaatccg
     7561 cggctatcgc attgagccga aagaaattga aacggtcatg ctcagcctca gcggaattca
     7621 agaagcggtt gtactagcag tttccgaggg cgggcttcaa gagctttgtg cgtattatac
     7681 ggcggatcaa gccattgaaa aagcagtgct ccgcaaccag ctttcacaaa cactgccgtc
     7741 tcatatgatt cctgctttct ttgtgcaggt ggacgccatt ccgctgacgg caaacgggaa
     7801 aaccgacaga aacgcattgc cgaagccgaa cacggaacaa tccggacgca ggacctcagc
     7861 cgcaccggaa acagagcttg aagagagcct atgccgcatt tggaagaaaa cgcttggcat
     7921 agaagcgatc ggcatcgacg acaatttctt tgatttaggc ggccattcat taaaagggat
     7981 gatgctcatt gccaacattc aggctgaatt ggagaaaacc gtaccgctta aagcgctgtt
     8041 cgagcagccg actgttcgcc agcttgcggc ttgcatggag gtatcgtcag tttcaggcgg
     8101 ccatcacgtg ctcaaaccgg ctgacaaaca ggagatgtac ccactgtcat ccgcgcagaa
     8161 acggatgtat gtactgaatc agcttgaccg gcagacgatc agctacaata tgccatctgt
     8221 tcttctgatg gaaggcgagc ttgatatttc acgtctgcgc gactcactca atcagcttgt
     8281 aaaccgccac gagtcattgc ggacatcatt tatggaagca aacggtgagc ctgttcagcg
     8341 cattattgag aaggcggcga ttgatcttca tgtgtttgaa gcgaaagaag atgaagcgga
     8401 acaaaagatc aaggacttta tccggccatt cgacttaaac ggcgcaccgc tcattcgcgc
     8461 ggcattgctt cgcatagaag cgaaaaaaca tttgctcctt ttagatatgc atcacatcat
     8521 cgcagacggg gtatcaagag gcatttttgt aaaagaattg gcgctgcttt acaaaggaga
     8581 gcagcttcca gagccggcgc ttcattataa ggatttcgcc gtttggcaaa atgaagctga
     8641 gcaaaaggaa cggatgaagg agcatgaggc gtactggctc tcggttcttt caggcgagct
     8701 gccggagctt gatcttccgc tcgattacgc ccgtccgcct gtgcaaagct ttaaagggga
     8761 cacggtccgt ttccgtacgg gaagcgagac ggcaaaggcg gtagaaaaac tgctcgcaga
     8821 aaccgggacg accttgcaca tggtgcttca cgctgttttc cacgtctttt taagcaaaat
     8881 ttccggacag cgggatatcg tcatcggctc cgttactgcc ggccggatga atgctgatgt
     8941 ccaagacatg ccgggaatgt tcgtcaatac gcttgcctta agaatggaag cggaagaaca
     9001 gcaaacattt gcagagctct tggagcaagc gaagcagacg aacctgtcag cgctggagca
     9061 tcaggaatat ccgtttgagg acttagtcaa tcagcttgat ctccctcggg atatgagccg
     9121 aaatccattg tttaacgtga tggtgacgac agaaaatcct gataaagaac agcttacatt
     9181 ggagaatcta agcatttcac cttacgagtc ccatcaggga acgtctaagt ttgatctgac
     9241 attaggcgga ttcactgatg aaaacggcat cggtctgcag ctcgaatatg cgactgatct
     9301 gttctcaaaa gaaacggctg agaaatggag cgaatacgtt ctgcgtttac taaaagctgt
     9361 tgcggaaaat ccgaaccagc cgctttccag cctatcactt gtcaccgaaa cagaaaaaca
     9421 aacgcttctc gaggcatgga cgggcaaaac gctgcctgtg ccgacagaca aaaccgttca
     9481 tcagctattc gaagagactg cccagcacca caaagatcgc ccggctgtta catacaaagg
     9541 ccagtcgtgg acgtacggcg agctgaatgc gaaggcgaac cgtcttgccc ggattctgat
     9601 cgactgcggt atcagcccgg atgagcgcgt cggcgttctc acgaagccgt cgctggaaat
     9661 gtccgccgcg gtgctcggcg tcttaaaggc gggagcggcg tttgtgccga ttgatcctga
     9721 ctatccggat cagcggattg actatatttt acaagacagc ggtgcgaagc ttctcttgaa
     9781 acaggaaggc atcgcagtgc cggacagcta tacgggggag atcattcttc ttgatggcaa
     9841 ccgtacgatt ctgagcctgc cgcttgatga aaacgatgag gaaaatccag agacggatac
     9901 aaacgcggag aacctggcgt acatgattta cacgtctgga acaaccggac agccgaaggg
     9961 tgtcatggtc gagcaccatg cgcttgtgaa cctgtgcttc tggcaccacg acgcgttcag
    10021 catgacagcg gaggaccgca gtgcgaagta cgcgggcttc ggattcgacg cctccatttg
    10081 ggagatgttc ccgacttgga caatcggcgc cgagcttcac gtcatagatg aagccatccg
    10141 acttgatatc gttcgcctga acgactattt cgaaacgaac ggcgtaacga tcactttcct
    10201 gccgacacag cttgcggaac agtttatgga gcttgagaac acatcactcc gcgtattgct
    10261 gacaggcgga gacaaactga agcgtgccgt gaaacagccg tacacgctcg ttaacaacta
    10321 cgggccgact gaaaatacgg tcgttgccac aagtacagag atcaatccgg aggaaggctc
    10381 gctttccatc gggcaggcga tcgccaatac gagagtgtac attcttggcg agggcaatca
    10441 ggtacagccg gaaggcgtag ccggagagct ttgcgtggcg ggacgcggac tggcgcgagg
    10501 ctatctgaat aaagaagacg aaaccgcgaa gcggtttgtc gctgatccgt ttgtgccggg
    10561 tgaacgcatg taccgcaccg gcgatttggt gaaatgggtg aacg
//
