LOCUS       NZ_MUXL01000003        13058 bp    DNA     linear   CON 09-SEP-2024
DEFINITION  Bacillus spizizenii strain AS2 scaffold_3, whole genome shotgun
            sequence.
ACCESSION   NZ_MUXL01000003
VERSION     NZ_MUXL01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus spizizenii
  ORGANISM  Bacillus spizizenii
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 440498)
  AUTHORS   Al-Bahry,S.N., Al-Wahaibi,Y.M., Al-Sayegh,A., Elshafie,A.,
            Al-Bemani,A.S. and Joshi,S.J.
  TITLE     Whole Genome Sequence of Heavy-oil Degrading and Biosurfactant
            Producing Bacillus subtilis AS2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 440498)
  AUTHORS   Al-Bahry,S.N., Al-Wahaibi,Y.M., Al-Sayegh,A., Elshafie,A.,
            Al-Bemani,A.S. and Joshi,S.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2017) Biology, Sultan Qaboos University, Al-Khod,
            Muscat 123, Oman
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_002155045.1
            Current Name                      :: Bacillus intestinalis
            Previous Name                     :: Bacillus subtilis
            Date Updated                      :: 2018-08-15
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. TYPE genome for current name
            A1 Genome (subject)               :: GCA_002027305.1
            A1 Name                           :: Bacillus intestinalis
            A1 ANI                            :: 99.23349%
            A1 Query Coverage                 :: 95.49%
            A1 Subject Coverage               :: 95.35%
            Analysis 2 (A2) :: Query vs. TYPE genome for previous name
            A2 Genome (subject)               :: GCA_000332645.1
            A2 Name                           :: Bacillus subtilis
            A2 ANI                            :: 94.5332%
            A2 Query Coverage                 :: 92.3%
            A2 Subject Coverage               :: 85.9%
            ##Taxonomic-Update-Statistics-END##
            ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 2.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002155045.1-RS_2024_09_09
            Annotation Date                   :: 09/09/2024 01:52:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,090
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 108
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 73 of 114
            Pseudo Genes (incomplete)         :: 53 of 114
            Pseudo Genes (internal stop)      :: 12 of 114
            Pseudo Genes (multiple problems)  :: 21 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:09:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 427440
            Orig. end                         :: 440498
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MUXL01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13058
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="CDPS"
                     /region_number="3"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..13058
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13058
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[10000:10747](-)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10747)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            complement(424..1356)
                     /gene="hprK"
                     /locus_tag="B0W20_RS12940"
                     /old_locus_tag="B0W20_12930"
     CDS             complement(424..1356)
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hprK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391380.1"
                     /locus_tag="B0W20_RS12940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B0W20_12930"
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="WP_014115306.1"
                     /transl_table=11
                     /translation="MAKVRTKDVMEQFNLELISGEEGVNRPITMSDLSRPGIEIAGYFT
                     YYPRERVQLLGKTELSFFEQLPEEDKKQRMDSLCTDVTPAIILSRDMPIPQELIDASEK
                     NGVPVLRSPLKTTRLSSRLTNFLESRLAPTTAIHGVLVDIYGVGVLITGKSGVGKSETA
                     LELVKRGHRLVADDCVEIRQEDQDTLVGNAPELIEHLLEIRGLGIINVMTLFGAGAVRS
                     NKRITIVMNLELWEQGKQYDRLGLEEETMKIIDTEITKLTIPVRPGRNLAVIIEVAAMN
                     FRLKRMGLNAAEQFTNKLADVIEDSEQDE"
     misc_feature    1468..1483
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     gene            1538..2728
                     /gene="nagA"
                     /locus_tag="B0W20_RS12945"
                     /old_locus_tag="B0W20_12935"
     CDS             1538..2728
                     /EC_number="3.5.1.25"
                     /GO_function="GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA]"
                     /GO_process="GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391381.1"
                     /locus_tag="B0W20_RS12945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B0W20_12935"
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="WP_061187845.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.7e-41, bitscore:
                     137.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAESLLIKGIEIVTENEVIKNGYVGINEGKISTISTERPKESYSK
                     EIQAPADSVLLPGMIDIHIHGGYGADTMDSSFSALDTMSSRLPEEGTTSFLATTITQEH
                     GHISQALVNARDWKAAEESSLLGAELLGIHLEGPFVSPKRAGAQPKEWIRPSDVALFKK
                     WQQEAGGLIKIVTLAPEEDRHFELIRYLKDESIIASMGHTDADSALLSEAAKAGASHMT
                     HLYNAMSPFHHREPGVIGTALAHDGFMAELIADGIHSHPLAAKLAFLAKGSTKLILITD
                     SMRAKGLKDGVYEFGGQSVTVRGKTALLSDGTLAGSILKMNEGARHMREFTNCSWTDIA
                     NITSANAAKQLGIFDRKGSVTEGKDADLVIVSSDCEVILTICRGNIAFISKEANQI"
     gene            2725..3453
                     /gene="nagB"
                     /locus_tag="B0W20_RS12950"
                     /old_locus_tag="B0W20_12940"
     CDS             2725..3453
                     /EC_number="3.5.99.6"
                     /GO_function="GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA]"
                     /GO_process="GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391382.1"
                     /locus_tag="B0W20_RS12950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B0W20_12940"
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="WP_061187844.1"
                     /transl_table=11
                     /translation="MKVIECQTYEELSQKAAKIAADTIKEKPDAVLGLATGGTPEGTYR
                     ELIRLHQTENLSFQNVTTVNLDEYAGLSSDDPNSYHFYMNHRFFQHIDSLPNRHFIPNG
                     NADDLEAECRRYEQVVESLGGTDIQLLGIGRNGHIGFNEPGTPFNSRTHVVTLNEQTRQ
                     ANARYFPSIDSVPKKALTMGIQTILTSKRILLLISGKSKAEAVKKLLEGDISEDFPASA
                     LHLHSDVTVLIDREAAALRP"
     gene            3471..4202
                     /locus_tag="B0W20_RS12955"
                     /old_locus_tag="B0W20_12945"
     CDS             3471..4202
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391383.1"
                     /locus_tag="B0W20_RS12955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B0W20_12945"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_072182561.1"
                     /transl_table=11
                     /translation="MKIDKQSPIPIYYQIMEQLKAQMKSGELLPDMPLPSEREYAEQFG
                     ISRMTVRQALSNLVNEGFLYRLKGRGTFVSKPKMEQALQGLTSFTEDMKSRGMTPGSRL
                     IDYQLIDSTEGLAAILGCGHPSPIHQITRVRLANDIPMAIESSHIPFELAGELNESHFQ
                     SSIYEHIERYNSIPISSAKQELEPSAATSQEASILGIQKGAPVLLIKRTTYLQNGTAFE
                     HAKSVYRGDRYTFVHYMDRLS"
     gene            complement(4221..8090)
                     /locus_tag="B0W20_RS12960"
                     /old_locus_tag="B0W20_12950"
     CDS             complement(4221..8090)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391384.1"
                     /locus_tag="B0W20_RS12960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B0W20_12950"
                     /product="metallophosphoesterase"
                     /protein_id="WP_087987574.1"
                     /transl_table=11
                     /translation="MRKYTVIASLFLSFLSVLSGGHHEPKAFPVINQEPFQTPHYIPLL
                     ENPPQIDVKAEMKSNLMIEAQVRDNEYQAFSAFLFYKQSNELGYKMVPMDPTPGAVRKF
                     FAQIPKRLIWNSELEYYIVVSNGKQRVESEMKKLKLEGYKADLAHIPELLITELAVDTK
                     NIGRADGYEFIEIYNTTDRTIDFKDYHIRYRYPKEGPDSDLIWRPEKKITIPSGETFVF
                     WLKPAGHPELTTEDFNRYYQTQLTEGKNLAVIEETEGMANTRPRAAVISTNTGKDISVA
                     HYRKHALRRLSSVLYKYPMDGTAELLNISIGEKNPSPGTVLQAQVPEQKRKIKLDKEKP
                     VIEDLTDRKPVRPAESIELRADIRDRSLVKTAAFYYRTDENKPFKRIFAEKDRNDNLYH
                     YIVYSPELIGKDQLEYYVTAGDGINEARTPVKMIDIKQTSKSHGLRMNIENRDTLSGTQ
                     FLKATTDGRTDSIRLWIDGKKQVTEPAMEKEVYFAFDTRKTNLYFKNAVTMEGKVLKVF
                     DDTTNKYRTYSVPLPETLLRKGKQLQHITIRSGSKVSPFDTAENRDDFLVKNARLVLSD
                     GTVIRDQRVSPEKELFIGDNQRSNKSWQFQFNLPDGLFTSQLLEWDTSKLSEGVHHIQA
                     SDGKENVSFVVRVDNSGPHIEPNITEGQTYKGNLILQADMYDKWSKIEEVKTSLDGESI
                     TLPYHTSSSELLPGKHSLKVTATDLAGNKTVIERTFKTEREHPDQPEVIDSEADTHRAK
                     LSVRVKDPTNDAMDVAFYRGFQYTARNNVKIFKHASLTEPPKSFVPESETPFTNKELER
                     VSAADGKTVSTEKKELFPYHRFEVAVDPSVDENDLAEAVWKGSSLPGRKVTMYVWNYRT
                     NEWQPVDSFVAKNDKPFMLKASVIASDFVRKSKMNVIVQDEIPPAKDMYTFVWMSDTQY
                     YAESYPHIFDKQTAWIKDNQKQLNIKYVFHTGDIVDDSADIRQWKNANRSMSVLDKSGI
                     PYGVLAGNHDVGHKDGSYRAFGKYFGSDRFDKKLHYGGSYKNNRGHYDLISSNGNDYIM
                     LYMGWGITNEDIAWMNQVLKKHPDRMAVLAFHEYLLVSGNRSPIGEKIFKKVVKPNPNV
                     VLVLSGHYHSAMRKTDELDDDGDGKPDRLVHQMLADYQGGPEGGQGYLRLLQFDQANDM
                     VHVSTYSPHLQDKNYYDADTHGDKDEFSLSLDLKPRIKKVETDYFECNVYTNEELGKRE
                     QVKSGDTAEFRWDHLEPHSVYYWYIIVEDSFNGKTKSPIWKFKTKKETYRPAPDQLDFR
                     HVSNP"
     gene            8255..8728
                     /locus_tag="B0W20_RS12965"
                     /old_locus_tag="B0W20_12955"
     CDS             8255..8728
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391385.1"
                     /locus_tag="B0W20_RS12965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B0W20_12955"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_087987575.1"
                     /transl_table=11
                     /translation="MDNSIHDELIQAIQQFALKRDKRVMQKVQIPSRDMSSQHLEHLRK
                     EWTLTQLHIVSCIHTSQNVNNSFLASRLHISKAAVSKAVQALLKHNIITVTKKPGNKKE
                     IFYTLTESGRELAALHEQLHEKAKEQYKQLFNEFSIDDLKTVTAFFNLWVKHM"
     gene            complement(8769..9986)
                     /gene="cypX"
                     /locus_tag="B0W20_RS12970"
                     /old_locus_tag="B0W20_12960"
     CDS             complement(8769..9986)
                     /EC_number="1.14.15.13"
                     /codon_start=1
                     /gene="cypX"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391386.1"
                     /locus_tag="B0W20_RS12970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B0W20_12960"
                     /product="pulcherriminic acid synthase"
                     /protein_id="WP_087987576.1"
                     /sec_met_domain="p450 (E-value: 7.7e-37, bitscore: 121.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQSIKLFSVLSDQFQENPYAYFSQLREEDPVHYEESIDSYFISR
                     YHDVRYILQHPDIFTTKSLVQRAEPVMRGPVLAQMHGKEHSAKRRIVVRSFVGDALDHL
                     SPLIKQNAENLLAPYLERGRIDLVNDFGKTFAVCVTMDMLGLDKRDHEKIAEWHSGVAD
                     FITSISQTPEARAHSLWCSEQLSQYLMPVIEERRVNPGSDLISILCTSEYEGMAMSDKD
                     ILALILNVLLAATEPADKTLALMIYHLLNNPEQMNDVLADRSLVPRAIAETLRYKPPVQ
                     LIPRQLSQDTVVGGMEIKKDTIVFCMIGAANRDPEAFERPDVFHIHREDLGIKSAFSGA
                     ARHLAFGSGIHNCVGAAFAKTEIEIVANIVLDKMRNIRLEEGFRYAESGLYTRGPVSLH
                     VAFDGA"
     gene            complement(10001..10747)
                     /gene="pchC"
                     /locus_tag="B0W20_RS12975"
                     /old_locus_tag="B0W20_12965"
     CDS             complement(10001..10747)
                     /EC_number="2.3.2.22"
                     /codon_start=1
                     /gene="pchC"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391387.1"
                     /locus_tag="B0W20_RS12975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B0W20_12965"
                     /product="cyclo(L-leucyl-L-leucyl) synthase"
                     /protein_id="WP_087987577.1"
                     /sec_met_domain="CDPS (E-value: 3.9e-83, bitscore: 273.2,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEMATERKPIDFTAEPLTQNCREIFERRQHVLVGISPFNSRFSE
                     DYIYRLIGWATAEFKSVSVLLAGHEAANLLEALGTPRGKAERKVRKEVSRNRRFAERAL
                     AAHGGDTKAIHTFSDFTENKAYQLMRQEVEHAFFEQPHFRHACLDVSREAIIGRARGIS
                     LMMEEVSEDMLNLAVEYVIAELPFFIGAPDILEVEETLLAYHRPWKLGEKISNHEFSIR
                     MRPNQGYLIVQEMAQMLSETRITSEG"
     gene            11186..11695
                     /gene="pchR"
                     /locus_tag="B0W20_RS12980"
                     /old_locus_tag="B0W20_12970"
     CDS             11186..11695
                     /codon_start=1
                     /gene="pchR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391388.1"
                     /locus_tag="B0W20_RS12980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B0W20_12970"
                     /product="pulcherriminic acid biosynthesis transcriptional
                     regulator PchR"
                     /protein_id="WP_014115315.1"
                     /transl_table=11
                     /translation="MSDLTKQMIYDIYVRLLHLNEQKANTSLQQFFKEAAEEDVIDIPK
                     NMTSIHVIDCIGQHEPINNAGIARKMNLSKANVTKISTKLIKEEFINSYQLTDNKKEVY
                     FKLTRKGRRIFDLHEKLHKKKELAFYQFLDSFSQDEQNAVLKFLEQLTSTLEAEQNDET
                     PDKPVK"
     gene            11717..12928
                     /locus_tag="B0W20_RS12985"
                     /old_locus_tag="B0W20_12975"
     CDS             11717..12928
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391389.1"
                     /locus_tag="B0W20_RS12985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B0W20_12975"
                     /product="MFS transporter"
                     /protein_id="WP_087987578.1"
                     /transl_table=11
                     /translation="MTQTKTKAVLILYIVCFSAFFASLSQNIYSPIIPIIKESFHVSAA
                     MVNLSVSVFMIVTAIMQIILGTIIDFKGARFVLIAGIMATAAASIGCAVTTDFTLFLIF
                     RMIQAAGSAALPLIAATTIGQLFTGSDRGSAMGTYQMLLSAAPVIAPVLGGFIGGAAGY
                     EGIFWMLTAISVVMLVTNSIIFPKDSPAGSKQQAQGNVFAHYKSIFTNRTGNVILTLSF
                     VLFFIYFAVIVYLPILLTEHYHIDVSIAGLLYLPLALSTIAGTFLFKKIQAKIALSTLF
                     IGSNVIAACSIMLFAVTHSVSLILMALTLALFGISMGVIPPLYSTMITNEFEHNRGSAI
                     GVFNFIRYTGMAAGPMISAYLLTLMPSAMSFGLLGLIFAALSFCLLPQMYPPQKRVKQK
                     KHHM"
ORIGIN
        1 cggccgatcg cctggccgag taaaatgctt ggcgcggcaa tatccgcaag cttccagaac
       61 gaaaggtttt tcactctcga aaacacataa cctgttaaaa tggcgccgat caatccgccg
      121 tgaatggcaa tgccgccttt ccaaatctta atgatttctc cgggatgtgc cgcataataa
      181 tcccattcaa atgcaacgta ataaattcgt gcgcaaataa tcgcaatcgg aatggcaaac
      241 aggacaagat cgataaacgt atctttctgc agcccccgtt tctcactttc cctcatcgca
      301 atccaaagcc caagcaaagc gccgagaccg ataatgattc cgtaccaatg gacggccaac
      361 ggccctagct gaaacgctat cggattgagt ggttctatcg cttcattcat ttaacgccaa
      421 ctcctattcg tcttgttcac tgtcttcaat gacatccgcc agcttattgg taaattgctc
      481 agcggcgttc agccccatcc gcttcaatct gaagttcatt gctgccactt cgatgatgac
      541 tgcgagattt cggcccggac ggacaggaat cgtcagtttt gtaatttctg tatcaatgat
      601 tttcatggtt tcttcctcaa gcccgagacg gtcgtattgc ttgccctgct cccaaagttc
      661 aagattcatg acaatcgtaa tccgtttatt gcttctgacc gcaccggcac caaacagcgt
      721 catcacgttg ataatgccaa gccctctgat ttcaaggaga tgctcaatta attcaggagc
      781 gtttccgaca agggtatcct gatcttcctg ccgaatttca acgcaatcat cggcgacaag
      841 gcggtgcccc cttttcacaa gctccagcgc ggtttcactt ttcccgacgc cgctctttcc
      901 tgtgatcagt acgccgacac cgtatatatc cacaagcaca ccgtgaatgg ctgtggttgg
      961 cgcaagtctg ctctcaagga agttggtcaa ccggcttgac agccttgtcg ttttcagcgg
     1021 cgatctgagg acaggcacgc cgtttttctc agaagcgtca atcagctcct gcgggatagg
     1081 catatctcta gaaagaataa tagctggtgt tacatccgta cacagagaat ccattcgctg
     1141 ctttttatcc tcttccggca gctgttcaaa gaaagaaagc tccgtttttc cgagaagctg
     1201 cacccgctct ctcgggtaat atgtaaaata tccggcaatt tcaatgcctg gtcttgataa
     1261 gtcactcatt gtaatcgggc ggttaactcc ttcttctccg ctgattaatt ccaaattgaa
     1321 ctgttccatt acgtcttttg tgcgaacctt tgccacgata tgttcctcct gttccgggct
     1381 gccccgagct tgctcacaat aatttcattt tatcactttc gggcttgaac ctaaaacaga
     1441 ttttataaaa gacaggaaaa cgccttagct ggtctagatc actagtctga aaaggggtaa
     1501 aataaatgta ttcatattcc agaaaggcgg atcacctatg gcagaaagtc ttcttatcaa
     1561 aggcatcgag atcgtgacag aaaacgaagt aatcaaaaat ggctatgtcg gaatcaatga
     1621 aggaaaaatc agcacaatct caacagaacg gcctaaagag tcttattcaa aagagattca
     1681 agcaccggca gattccgttt tgcttcccgg gatgattgac attcatattc acggcggcta
     1741 tggcgcagat acaatggatt caagcttttc agccctcgac accatgtcgt caaggctgcc
     1801 cgaagaaggc acgacgagct ttttggcaac aacgattact caggagcatg ggcacatttc
     1861 tcaagctttg gtaaatgcaa gagattggaa agcagctgag gaatcctctt tattaggtgc
     1921 ggaactgctc ggcattcatt tagagggccc ctttgtttca cctaaaagag ccggagcgca
     1981 gccaaaggaa tggatcaggc cttcggatgt tgcgcttttc aaaaagtggc agcaggaggc
     2041 gggcggactg atcaaaattg tcacactcgc gccggaagag gaccggcatt ttgaactgat
     2101 ccgctatttg aaagacgaat cgattatcgc gtcgatgggg cacacagatg ccgactccgc
     2161 attactgtcg gaggccgcga aagcaggcgc gagccatatg acccatcttt acaacgcgat
     2221 gagcccgttt catcaccgag agcccggagt gatcggaacg gcacttgccc atgatgggtt
     2281 tatggcagag cttattgccg acggcattca ctcccatcct ttagcggcaa agcttgcttt
     2341 cttggcaaaa ggcagcacca agctcatttt aattaccgat tcaatgaggg caaaaggcct
     2401 gaaagacggc gtatatgagt tcggcggcca aagtgtgacg gtaagaggaa aaacagctct
     2461 tctttcagac ggaacacttg ccggttcgat cctcaaaatg aacgaaggcg cacggcatat
     2521 gcgtgagttt acaaattgtt cttggacgga tatagctaat ataacttctg caaacgcggc
     2581 caaacagctg ggcatctttg accgaaaagg cagtgtgaca gagggaaaag atgcggatct
     2641 tgtcattgtc agcagtgatt gcgaggtgat tctcaccatt tgccgcggaa acattgcatt
     2701 tatatccaag gaggcgaacc agatatgaaa gtaatagaat gtcaaacata tgaagagcta
     2761 agccaaaaag cagccaaaat agcggcagac acaatcaagg aaaaacctga cgccgttctc
     2821 ggtttagcga caggcggcac accggaaggc acgtatcgcg agctgatccg cctgcaccaa
     2881 actgagaatc tctcatttca aaacgtcacc acggttaatt tggatgaata cgccggactt
     2941 tcaagcgatg acccgaacag ctatcacttc tatatgaatc atcgtttttt tcagcatatc
     3001 gatagccttc caaaccggca ttttatcccg aatggaaatg cagacgacct ggaagccgaa
     3061 tgcagacggt acgaacaagt agtcgaatcc ctcggcggca ctgacattca gcttctcggc
     3121 atcggccgaa acggacatat cggctttaac gaaccgggaa cgcctttcaa ctcgcggaca
     3181 cacgttgtga ccttaaatga acaaacccgc caggcgaatg ccagatattt tccttcaata
     3241 gacagtgtgc caaaaaaagc gctcacaatg ggaatccaga cgattctcac aagcaagcgc
     3301 attctgctgc ttatttccgg caaaagcaag gcagaagcag tgaaaaagct gttagaagga
     3361 gacataagtg aagatttccc tgcatctgct ttgcacctgc attccgatgt aacggttttg
     3421 attgatcgtg aagcagcggc attaagacct tgaaaggaac atgctgactt atgaagatcg
     3481 ataaacaatc gcctattccg atttattatc agattatgga acaattaaaa gcccaaatga
     3541 agagcggaga gctgctgccg gatatgcctc ttccctctga gcgtgaatat gccgaacaat
     3601 ttgggatcag ccggatgacg gttcgccagg ctctttctaa tttagtcaat gaaggctttc
     3661 tctatcgcct taaagggcgg ggaacctttg tcagcaagcc aaaaatggaa caagcactcc
     3721 aagggctgac aagctttacc gaggatatga aaagccgcgg gatgacaccg ggcagcaggc
     3781 tcattgacta tcagcttatt gattcaacgg aggggctcgc cgctatatta ggttgcgggc
     3841 acccctcccc tatccatcaa atcactcggg tgcgactggc aaatgatatt ccgatggcga
     3901 tcgaatcctc gcatattccg tttgagcttg cgggtgaatt aaatgaatcg cattttcagt
     3961 cttcgattta cgagcatatt gaaaggtaca acagcatacc gatttccagt gcaaaacagg
     4021 agcttgaacc aagcgctgca acatctcaag aagcaagcat tctcggcatt caaaaggggg
     4081 cgcctgtcct gttaatcaaa cgaacaacat atttacagaa cggaactgct tttgaacatg
     4141 ccaaatctgt atacagaggc gaccgttata catttgtcca ctatatggat cggctttcat
     4201 aaaaaaacct ccaatccttt ttaaggattg gagacatggc gaaaatctaa ctggtctggc
     4261 gccggacgat atgtctcttt tttcgtcttg aacttccaga tcggtgattt cgttttgccg
     4321 ttaaaactgt cttctacaat gatgtaccaa taataaacag aatgcggctc gagatgatcc
     4381 cagcggaatt cagcagtatc cccgctcttc acttgctccc gttttccgag ctcttcattg
     4441 gtatatacat tacactcaaa ataatcggtt tccacctttt tgatcctcgg cttcaaatca
     4501 agcgaaagag aaaactcatc cttgtcaccg tgggtatccg catcatagta gtttttatct
     4561 tgtaaatgcg gagagtacgt cgacacatgc accatatcat tcgcctggtc aaattgcagc
     4621 aaacgcaaat acccttgccc gccttctggc ccgccctgat aatcggccag catttgatgc
     4681 acgaggcgat ccggttttcc atcaccatca tcatcaagct catcagtttt tctcatcgca
     4741 ctatgataat gtccgcttaa caccagcacg acattcggat tcggcttcac gactttttta
     4801 aatattttct ctccgatcgg gcttctgttt ccgctgacca gcaaatactc atgaaaagct
     4861 aaaacagcca tccggtcagg gtgcttcttc agtacctgat tcatccatgc gatgtcttca
     4921 ttggtaatgc cccatcccat gtatagcatg atgtaatcat tgccgttgct tgaaatcagg
     4981 tcgtaatgcc ctcggttatt tttataggaa ccgccataat gcaatttttt atcaaaacga
     5041 tcactgccaa agtatttgcc aaacgccctg taggagccgt ccttatgccc gacatcgtgg
     5101 ttaccggcca gcacgccgta aggaatcccg cttttatcca gaacggacat tgaccgattc
     5161 gcgtttttcc actgtctgat atcagctgaa tcatctacaa tatcacccgt gtgaaacaca
     5221 tatttgatat tgagttgctt ttgattatct tttatccatg cagtttgttt atcaaaaata
     5281 tgcggatagc tttcggcgta atactgtgta tcggacatcc agacaaatgt gtacatatcc
     5341 ttcgcaggag gaatttcatc ctgtacgatc acattcatct tcgacttccg tacaaaatcg
     5401 gaggcgatca cagacgcctt cagcataaac ggtttgtcat tctttgcaac aaagctgtca
     5461 accggctgcc actcgtttgt ccgataattc caaacataca tcgtcacctt tctccccggc
     5521 aaagaacttc ccttccagac tgcttccgcc aagtcattct catcaactga cggatcaacc
     5581 gccacttcaa agcgatgata agggaaaagt tccttctttt ctgtcgatac tgtttttccg
     5641 tcagcagcag ataccctctc caattcttta ttcgtaaatg gggtctcgct ttccggcaca
     5701 aagcttttcg gcggttcagt cagtgacgca tgcttgaata ttttcacatt gtttcgcgct
     5761 gtatattgaa atcctctata aaaagcgaca tccatggcgt cattcgtcgg atccttcacc
     5821 cgcacagaaa gcttagccct atgcgtgtcg gcttcgctgt ctatgacctc cggctgatct
     5881 ggatgctctc gttctgtttt aaacgtccgc tcaatgactg ttttattgcc cgcgagatcc
     5941 gttgctgtca cttttagcga atgttttccc ggcaaaagtt cagaagatga cgtatggtat
     6001 gggagtgtaa tgctttcacc atctaaagac gtcttcactt cctcaatttt gctccattta
     6061 tcatacatat ccgcctgcag aatgaggttt cctttgtacg tttgtccctc ggtgatattc
     6121 ggctcaatgt gaggccctga attatctacc cgcacaacaa agcttacatt ttccttgccg
     6181 tcgcttgctt gaatatggtg gacgccttca gaaagctttg atgtatccca ctccagcagc
     6241 tgtgacgtaa acagtccgtc aggcagattg aattgaaatt gccagctttt attcgagcgc
     6301 tgattatctc caataaagag ttccttctca ggagaaaccc tctgatccct gatcaccgta
     6361 ccatccgata acaccagtcg ggcgtttttg actaagaaat catcacggtt ttcagccgta
     6421 tcaaacggcg atacctttga acctgatcgt atcgtaatat gctgcagctg cttccctttg
     6481 cgaagcagcg tctccggcaa tggaacagaa taggtgcggt atttatttgt cgtatcatcg
     6541 aacaccttta atactttgcc ttccatcgtt accgcgtttt taaagtaaag attcgttttt
     6601 ctcgtatcga acgcaaaata cacttctttt tccatagcgg gctctgtcac ttgttttttt
     6661 ccgtcaatcc acagcctaat gctgtcagtg cgcccgtctg tcgtcgcttt tagaaactga
     6721 gttcccgata gcgtatctct gttttcaata ttcatccgca aaccgtgcga cttgcttgtt
     6781 tgtttaatat caatcatttt gaccggggtt cttgcttcat tgatcccgtc ccctgcagtc
     6841 acgtaatact caagctgatc cttcccgatc agttcaggcg aatacacaat ataatgatag
     6901 agattgtcgt tgcggtcttt ttcagcgaaa atgcgcttaa acggtttgtt ttcatctgtc
     6961 cgatagtaga aagccgccgt tttcacaaga ctgcgatccc gtatgtcagc acgcagctca
     7021 atgctttcag caggacggac aggcttgcgg tcagtcaaat cctcaatgac tggtttttct
     7081 ttatcaagct tgatttttcg tttctgctca ggcacctgcg cctgtaatac agttccgggg
     7141 gacggattct tttctccgat tgaaatattg agaagttctg cggtaccgtc catcggatac
     7201 ttgtaaagca cagacgacag ccggcgcagc gcatgtttcc gataatgtgc aaccgaaata
     7261 tcttttcctg tattcgttga tatcacggct gcacgcggtc tggtattggc catgccctct
     7321 gtctcttcta tcaccgcaag atttttccct tccgttaatt gtgtctgata atatcgattg
     7381 aaatcctccg tcgtcaattc tggatgtccg gcaggcttca gccaaaacac aaacgtttca
     7441 ccggatggaa tcgtgatctt tttttccggt ctccaaatca gatcggagtc aggcccttct
     7501 ttcggatagc ggtaacggat gtgatagtct ttaaaatcaa tcgttctgtc agtcgtatta
     7561 taaatctcaa taaattcata accgtctgca cgtccaatgt tctttgtatc aactgctagc
     7621 tctgtgatca gaagctccgg aatatgggca agatcagcct tatatccttc gagcttcagt
     7681 ttcttcatct ccgactcgac ccgctgcttc ccattggaaa ccacaatgta atactcaagc
     7741 tcgctgttcc aaatcaatcg tttcggaatt tgcgcgaaaa acttgcggac cgctccgggt
     7801 gtcggatcca tcggcaccat tttatagccc agctcattac tttgtttata aaacagaaag
     7861 gcagaaaatg cttgatactc attgtcacgc acttgagcct caatcatcag attgcttttc
     7921 atttcagctt tcacatcgat ttgaggagga ttttcaagca agggtatgta atgaggcgtt
     7981 tggaatggtt cctgatttat aacaggaaaa gctttcggtt catgatggcc gccagacaaa
     8041 actgaaagaa aagacaagaa taaagaagca ataacagtgt atttcctcat caaaacacct
     8101 cccttttcat gtattttgcg tctgaacagg gaagcttatg agttcatttt tgtgcataaa
     8161 gaacaaaaaa gcgtctgcct cttgaagatt ccactattac ccagtaaact aaggatagtt
     8221 aaccagttaa caattaaatc agagaggcaa attgatggac aacagtattc atgatgaatt
     8281 aattcaagct atacaacaat tcgctttaaa aagggataaa cgtgtcatgc aaaaggtaca
     8341 gataccatcc cgcgacatgt cctcacaaca tctcgaacac ctcagaaaag aatggacatt
     8401 gacccagctt cacattgttt cctgtataca taccagtcaa aatgtgaaca acagcttctt
     8461 agcatcccgc ttacatattt caaaagccgc tgtttcaaaa gcagttcaag ctttgttgaa
     8521 acacaatatc atcacagtga caaaaaagcc cggcaacaaa aaagaaattt tttatacgct
     8581 aactgaaagc ggcagagaac tggcggcatt acatgaacag cttcatgaga aagcaaagga
     8641 gcaatacaaa cagctcttca atgaattttc aattgatgat ttaaaaacag tgactgcgtt
     8701 tttcaacttg tgggtaaaac atatgtaaaa aaagcctccc ccgcatggga gagacctttg
     8761 aaatcttatt acgccccgtc aaacgcaacg tgaagtgaga caggtccgcg tgtatacaga
     8821 ccggactcag cataacgaaa accttcctct aatctgatat tccgcatctt atccagcaca
     8881 atattggcta caatctcgat ttcggttttg gcaaaagctg ctcctacaca gttatggatg
     8941 cctgatccga aagcgagatg ccgggcggcg ccgctaaaag cgctcttgat gccgagatct
     9001 tcccgatgaa tatgaaacac gtcaggacgt tcaaacgctt cagggtcccg gttagccgct
     9061 ccgatcatac aaaaaacaat cgtatctttt ttgatttcca taccgccgac caccgtatcc
     9121 tgggacagct gccgcggaat cagctgaacc ggcggtttat aacgcaacgt ctccgcaatg
     9181 gctctcggca ctaacgaacg gtcagccaaa acatcattca tctgctcggg attgttgagc
     9241 aaatgataaa tcatcagtgc cagcgtctta tctgccggtt ccgttgcggc taacagcaca
     9301 ttgagaatca gtgccagtat atccttgtcc gacatcgcca tgccttcata ttcagaagta
     9361 cataggatgg aaattaaatc agatcccgga ttgacgcggc gttcttcgat gaccggcatc
     9421 aagtattgag aaagctgttc gctgcaccat aaagaatgcg cccgcgcttc tggagtctga
     9481 gagatactcg taataaaatc ggcaaccccg ctgtgccact cagcgatttt ttcatgatct
     9541 cttttatcca gcccgagcat gtccatcgtg acacacactg caaacgtctt tccaaaatca
     9601 ttgacgagat cgattcttcc tctttcaaga taaggtgcta acaagttttc tgcattttgt
     9661 ttaatcaatg gagacagatg atccaatgcg tcccctacaa agcttctcac tacaattctt
     9721 cttttagcgg agtgttcttt cccatgcatt tgggccagca cagggccgcg catgaccggt
     9781 tcagcacgct gaacaagtga ttttgtcgtg aaaatatcag gatgctgaag gatatagcgg
     9841 acatcgtgat agcggctgat aaaatagctg tctatcgact cttcataatg aacaggatct
     9901 tcctcccgca gctgtgaaaa ataagcatat ggattttctt gaaattgatc agaaagcacg
     9961 ctaaacaatt taatggattg attcatgtta actcccccta tcatccttca gatgtgatcc
    10021 gtgtttcaga aagcatctgc gccatttctt gcacaatgag atacccttga ttcggccgca
    10081 tacgaataga aaattcatgg ttactgatct tctcacccag cttccacgga cgatgataag
    10141 caaggagcgt ctcttccacc tcaagaatat ccggagctcc gataaaaaac ggcagctcag
    10201 caatgacata ttccacagct aaattcagca tatcctcact gacttcttcc atcatcaaac
    10261 tgattccgcg tgcacgcccg ataatcgctt cacgagacac gtccaaacaa gcatgccgaa
    10321 aatgtggctg ctcaaaaaac gcatgttcaa cttcttgtct catcagctga taggctttgt
    10381 tttccgtgaa atcagaaaat gtatgaatcg ccttcgtgtc cccgccatga gccgcaagag
    10441 ccctttctgc aaatctcctg tttcgcgata cctctttcct tacctttcgt tcagccttgc
    10501 ctctgggagt tccgagcgct tcaagaaggt tagccgcctc atgccccgca agcaaaacgg
    10561 aaacgctttt aaattcagct gtcgcccatc caatcaatct gtaaatataa tcttcagaaa
    10621 acctgctgtt aaatgggctg atccccacca aaacatgctg gcgccgttca aatatttctc
    10681 tgcaattttg tgtcagtggc tcagcagtaa aatctatagg ctttctttcc gtcgccattt
    10741 cggtcatctc attcacccct aaaaaactta tcccgcttta agtcatcacc ctcataaaga
    10801 gttggaacaa ctggcacaac tgaacaagac gaatggcagg cagcacttaa agttaccaag
    10861 cgaattatgt taagattcaa acttctatgt tcatttttca aggtgcatat cataattaat
    10921 gtttactagt aaacattagt cattttatcc cttattcctc tacccgtcaa tataatccgt
    10981 acccaaatat ttgaaaataa atgtaaatgt cacatttttc acacactttt tacactcata
    11041 ttcagcatga cgcaacacca aataaagctt aaacaccagc aattgacaac gaaaacaaga
    11101 aaagataaaa taatattgtt caccggtaaa caatattgtt tattgtttca tcgtaaacta
    11161 ttattcctaa gaaagaaggt agcctatgtc tgatttgacg aagcagatga tatacgacat
    11221 atatgtgaga ctgctgcacc ttaatgaaca aaaagcgaat acttcacttc agcaattttt
    11281 taaggaggcc gcagaggagg atgtgatcga tatccctaaa aacatgacaa gcattcatgt
    11341 cattgactgt atcggccagc atgaacccat taacaatgcg ggaatcgcca gaaaaatgaa
    11401 cttatcgaaa gcgaatgtaa cgaaaatcag cacaaaactg atcaaggaag aattcattaa
    11461 cagctatcag ctgacagata acaaaaaaga agtgtatttc aaattaaccc gtaaaggcag
    11521 acggattttc gacttgcatg agaaattgca taaaaaaaag gagctggctt tttaccaatt
    11581 cctcgattca ttttcacaag acgaacaaaa tgctgtattg aaatttctag agcagctgac
    11641 atctacactt gaagcagaac aaaacgatga gactccggac aaacctgtaa agtaaacaaa
    11701 aaggcggtgt acacttatga ctcaaacaaa aacaaaagcg gtattgatct tatacattgt
    11761 ttgcttcagc gcattttttg catctttaag ccaaaacatt tattcaccta ttatcccgat
    11821 tattaaagaa tcattccatg tttccgcagc aatggtaaac ctgtcagtct cagtttttat
    11881 gattgtgaca gcgattatgc aaattatatt agggaccatc atcgatttta aaggcgcgcg
    11941 gtttgtcttg attgccggaa ttatggcaac ggcagctgcc agcatcggct gtgcggtaac
    12001 cactgacttt actttgtttc tgatattcag aatgatacag gctgcgggtt ctgcagcact
    12061 gcctcttatt gctgccacaa cgatcggaca gctgtttaca ggcagtgacc gcgggagtgc
    12121 aatgggaaca tatcaaatgc tcctgtccgc cgcgcctgtt atcgcacccg ttctcggggg
    12181 attcataggc ggagcagccg ggtacgaagg gatcttttgg atgcttacgg ccatctctgt
    12241 cgttatgctg gtgacaaaca gcatcatctt tcctaaggac tctcccgctg gatctaagca
    12301 gcaagcccaa ggcaatgtgt tcgctcatta caaatcaata tttacaaatc gaacaggaaa
    12361 cgtcattttg accttaagtt ttgtcctctt tttcatttat tttgcggtga tcgtctacct
    12421 cccgatattg ctgacggagc actatcatat tgatgtgagc atagcaggat tgttatattt
    12481 gccactggcg ctaagtacga ttgccggtac gtttctgttt aaaaaaatac aggcaaaaat
    12541 cgcgctgagc accttgttta tcggcagcaa tgtaatcgca gcctgcagta tcatgttgtt
    12601 cgccgttaca cattccgttt ctctcattct catggctctc acactggcac tgtttggcat
    12661 ctcgatgggg gtcatccctc ccttgtactc cacaatgatc accaatgaat ttgaacacaa
    12721 tagagggagt gcaatcggag tttttaactt tatcagatat acaggcatgg cggcaggtcc
    12781 gatgatatcc gcctatctgc tcacactgat gccgtctgcc atgtcctttg gactcttagg
    12841 ccttatattt gccgcattga gcttttgcct tctgccgcaa atgtatccgc cgcagaagcg
    12901 cgtaaaacaa aaaaaacacc acatgtaaaa aaagctgcct tctggggact gaccccataa
    12961 gatgagacaa ataaaaacac cttcaagttt gaaaacggat gattgttatc cgaaattaga
    13021 cttggaggtg ttttttctat ggggacaaga gtgagtta
//
