GCF_002148095.1 - 21 region(s)

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Location: 75,951 - 119,532 nt. (total: 43,582 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene overview

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KnownClusterBlast

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TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidase

BK765_RS00585: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

Transferases: phosphorylase phosphotransferase

BK765_RS00550: thiamine-phosphate pyrophosphorylase

smcogs: SMCOG1280: thiamine-phosphate pyrophosphorylase

BK765_RS00575: Glucose-specific phosphotransferase enzyme IIA

smcogs: SMCOG1145: Glucose-specific phosphotransferase enzyme IIA

Hydrolases: glucosidase

BK765_RS00580: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

Ligases: ligase

BK765_RS00535: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0001132.4	0.60	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.60	NRPS	AM-toxin	Alternaria alternata
BGC0002286.3	0.60	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.60	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae
BGC0001758.4	0.60	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0002174.2	0.59	NRPS	ε-Poly-L-lysine	Epichloe festucae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0001132.4	0.60	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.60	NRPS	AM-toxin	Alternaria alternata
BGC0002286.3	0.60	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.60	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae
BGC0001758.4	0.60	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0002174.2	0.59	NRPS	ε-Poly-L-lysine	Epichloe festucae

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 25.04 out of 26.12

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.78 out of 27.78

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 22.91 out of 25.88

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 22.91 out of 25.88

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.96 out of 20.97

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.81 out of 20.97

Confidence: medium

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.69 out of 21.55

Confidence: strong

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.55 out of 24.78

Confidence: medium

Additional weak hits (7):

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.13 out of 25.88

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.13 out of 25.88

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.70 out of 25.75

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.89 out of 30.25

Confidence: weak

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.11 out of 20.47

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 24.78 out of 31.73

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 24.98 out of 33.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 75950 - 119532:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BK765_RS00535: X

AMP-binding (225..621): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LWMPFDASVWEAALAIGGEHNVYGPTECTVVTTT
Nearest Stachelhaus code(s):: Leu DAWYLGNVVK (71% 8Å match)
Stachelhaus code match:: 70% (weak)

TFBS definitions

Regulator	Description
ArcA	Repressor of carbon oxidation
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
LexA variant 2	Repressor of DNA damage response
LuxO	Repressor of luminescence
PsrA	Oleic acid responsive regulator

NZ_MOOT01000009.1 - Region 1 - RiPP-like

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Location: 1 - 8,814 nt. (total: 8,814 nt) Show pHMM detection rules used

Region on contig edge.

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000558.5	0.49	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0000590.5	0.46	ribosomal	microcin N	Escherichia coli
BGC0000617.5	0.44	ribosomal	coagulin	Bacillus coagulans
BGC0002442.3	0.43	ribosomal	toyoncin	Bacillus toyonensis
BGC0002579.2	0.43	ribosomal	carnobacteriocin XY	Carnobacterium maltaromaticum
BGC0002306.2	0.43	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0001863.4	0.42	ribosomal, terpene	bacillicn CER074	Bacillus mycoides
BGC0001861.4	0.42	ribosomal	bacillicin BAG2O	Bacillus cereus BAG2O-1
BGC0001862.4	0.41	ribosomal	pallidocin	Aeribacillus pallidus
BGC0000624.3	0.40	ribosomal	salivaricin CRL1328 α peptide, salivaricin CRL1328 β peptide	Lactobacillus salivarius

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000558.5	0.49	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0000590.5	0.46	ribosomal	microcin N	Escherichia coli
BGC0000617.5	0.44	ribosomal	coagulin	Bacillus coagulans
BGC0002442.3	0.43	ribosomal	toyoncin	Bacillus toyonensis
BGC0002579.2	0.43	ribosomal	carnobacteriocin XY	Carnobacterium maltaromaticum
BGC0002306.2	0.43	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0001863.4	0.42	ribosomal, terpene	bacillicn CER074	Bacillus mycoides
BGC0001861.4	0.42	ribosomal	bacillicin BAG2O	Bacillus cereus BAG2O-1
BGC0001862.4	0.41	ribosomal	pallidocin	Aeribacillus pallidus
BGC0000624.3	0.40	ribosomal	salivaricin CRL1328 α peptide, salivaricin CRL1328 β peptide	Lactobacillus salivarius

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.94 out of 23.79

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.71 out of 25.88

Confidence: medium

Additional weak hits (2):

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 18.71 out of 29.51

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 18.63 out of 30.11

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
PsrA	Oleic acid responsive regulator

NZ_MOOT01000010.1 - Region 1 - NRPS-like,RRE-containing

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Location: 1 - 17,689 nt. (total: 17,689 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: lyase

BK765_RS01865: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.58	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.57	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001641.4	0.55	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.55	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.54	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.54	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.54	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.54	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.54	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.54	NRPS	AbT1	Aureobasidium pullulans

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.62	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001641.4	0.61	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.61	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.61	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.61	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.61	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.60	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.60	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 20.94 out of 25.31

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 20.67 out of 25.31

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.50 out of 27.78

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 23.85 out of 30.25

Confidence: medium

Additional weak hits (1):

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.40 out of 20.01

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

RREFinder

TFBS Finder

RRE predictions

: RRE type: Pantocin_Microcin_RRE; Location: 0 - 94; Score: 93.6

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
ColR	Phenol-responsive regulator

NZ_MOOT01000027.1 - Region 1 - terpene

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Location: 125,479 - 147,332 nt. (total: 21,854 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BK765_RS04995: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BK765_RS05000: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

Lyases: lyase

BK765_RS05025: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000917.3	0.53	other (other)	molybdenum cofactor	Rhodobacter capsulatus
BGC0000888.5	0.49	other (other)	bacilysin	Bacillus sp. CS93
BGC0001928.2	0.46	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0000916.5	0.46	other (other)	molybdenum cofactor	Staphylococcus carnosus
BGC0000812.3	0.45	other (other)	huperzine A	Shiraia sp. slf14
BGC0000231.5	0.42	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001605.3	0.42	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0001197.5	0.42	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0000234.5	0.41	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002138.2	0.40	PKS	fuelimycin A, fuelimicin B, fuelimicin C	Streptomyces albireticuli NRRL B-1670

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000917.3	0.53	other (other)	molybdenum cofactor	Rhodobacter capsulatus
BGC0000888.5	0.49	other (other)	bacilysin	Bacillus sp. CS93
BGC0001928.2	0.46	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0000916.5	0.46	other (other)	molybdenum cofactor	Staphylococcus carnosus
BGC0000812.3	0.45	other (other)	huperzine A	Shiraia sp. slf14
BGC0000231.5	0.42	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001605.3	0.42	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0001197.5	0.42	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0000234.5	0.41	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002138.2	0.40	PKS	fuelimycin A, fuelimicin B, fuelimicin C	Streptomyces albireticuli NRRL B-1670

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene	hopanoid		C30	hopanoid skeleton	unknown
triterpene	polypodane		C30	polypodane skeleton	unknown

Genes with terpene domains:

BK765_RS04985: T2TS

Domain 1 (4 - 614): Type 2 terpene synthase

Subtype: Squalene substrate

Reactions:: squalene → hopanoid; squalene → polypodane

Transcription Factor Binding Sites

Binding sites found:

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 26.42 out of 30.22

Confidence: medium

Additional weak hits (1):

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 24.61 out of 36.54

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description
ClgR	Caseinolytic protease gene regulator

NZ_MOOT01000030.1 - Region 1 - NRPS,NRPS-like

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Location: 1 - 4,001 nt. (total: 4,001 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

BK765_RS06330: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BK765_RS06335: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.84	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.66	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.64	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.62	NRPS	AbT1	Aureobasidium pullulans
BGC0001641.4	0.61	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001240.4	0.61	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0000342.4	0.60	NRPS	enniatin	Fusarium equiseti

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.66	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.65	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.65	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.65	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001240.4	0.64	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0000342.4	0.63	NRPS	enniatin	Fusarium equiseti

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 25.71 out of 29.38

Confidence: medium

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 23.29 out of 29.25

Confidence: medium

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 4001:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asn)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BK765_RS06330: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (507..844): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWCSFDLTITGSKLVVGGEINEYGPTEAVVGCTN
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

BK765_RS06335: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (0..255): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWCSFDLTITGSKLVVGGEINEYGPTEAVVGCTN
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
FuR variant 1	Ferric Uptake Regulator
SypG	Regulator of biofilm formation and host colonization

NZ_MOOT01000031.1 - Region 1 - NRPS

Download region SVG

Location: 1 - 31,187 nt. (total: 31,187 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

BK765_RS31855: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Ligases: ligase

BK765_RS06340: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BK765_RS06345: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.73	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.71	NRPS	AM-toxin	Alternaria alternata
BGC0000342.4	0.71	NRPS	enniatin	Fusarium equiseti
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.69	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.69	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0000307.4	0.68	NRPS	AbT1	Aureobasidium pullulans
BGC0001758.4	0.68	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.73	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.71	NRPS	AM-toxin	Alternaria alternata
BGC0000342.4	0.71	NRPS	enniatin	Fusarium equiseti
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.69	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.69	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0000307.4	0.68	NRPS	AbT1	Aureobasidium pullulans
BGC0001758.4	0.68	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.63 out of 27.55

Confidence: medium

Additional weak hits (2):

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 20.31 out of 29.51

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.88 out of 30.98

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 31187:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (NH2 - Thr - X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BK765_RS06340: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (504..841): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWCSFDLTITGSKLVVGGEINEYGPTEAVVGCTN
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

BK765_RS06345: NH2 - Thr - X

Search NORINE for peptide: strict or relaxed

CAL_domain (168..484): NH2

minowa: NH2

Prediction, score:

NH2: 129.0
Acetyl-CoA: 45.2
AHBA: 12.8
fatty_acid: 1.5
shikimic_acid: 0.0

AMP-binding (1174..1580): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LAAHFDFSVWEGNQIFGGEINMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2243..2651): X

nrpys: Dpr

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HAKAFDVSVWQSETVLNGEINACGATESSDDVNH
Nearest Stachelhaus code(s):: Dpr DVWEMNADDK (68% 8Å match)
Stachelhaus code match:: 90% (moderate)

TFBS definitions

Regulator	Description
GnfM	Regulator of nitrogen-fixation genes

NZ_MOOT01000033.1 - Region 1 - NRPS

Download region SVG

Location: 1 - 2,615 nt. (total: 2,615 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

BK765_RS06600: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.64	NRPS	AbT1	Aureobasidium pullulans
BGC0000342.4	0.63	NRPS	enniatin	Fusarium equiseti
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.63	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.62	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.62	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.64	NRPS	AbT1	Aureobasidium pullulans
BGC0000342.4	0.63	NRPS	enniatin	Fusarium equiseti
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.63	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.62	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.62	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed domain annotation

Selected features only

Show module domains

NZ_MOOT01000040.1 - Region 1 - NRP-metallophore,NRPS

Download region SVG

Location: 82,714 - 134,462 nt. (total: 51,749 nt) Show pHMM detection rules used

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

BK765_RS08410: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

BK765_RS08415: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

BK765_RS08420: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

BK765_RS08485: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BK765_RS08565: Antibiotic biosynthesis monooxygenase

smcogs: SMCOG1258: Antibiotic biosynthesis monooxygenase

Transferases: PPTase

BK765_RS08520: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: hydrolase protease

BK765_RS08500: isochorismatase

smcogs: SMCOG1027: isochorismatase

BK765_RS08545: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: hydratase

BK765_RS08430: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Isomerases: isomerase

BK765_RS08490: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

BK765_RS08495: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BK765_RS08505: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BK765_RS08570: Pseudouridine synthase

smcogs: SMCOG1154: Pseudouridine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001185.5	1.05	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0000309.5	0.89	NRPS	bacillibactin	Bacillus subtilis subsp. subtilis str. 168
BGC0000401.5	0.87	NRPS	paenibactin	Paenibacillus elgii B69
BGC0002075.3	0.86	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0000343.5	0.85	NRPS	enterobactin	Pseudomonas sp. J465
BGC0001132.4	0.77	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.74	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.72	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001185.5	0.80	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0000309.5	0.75	NRPS	bacillibactin	Bacillus subtilis subsp. subtilis str. 168
BGC0000401.5	0.74	NRPS	paenibactin	Paenibacillus elgii B69
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0000343.5	0.74	NRPS	enterobactin	Pseudomonas sp. J465
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 24.13 out of 27.78

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.53 out of 26.06

Confidence: strong

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 24.21 out of 30.25

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.59 out of 23.05

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.53 out of 29.38

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 25.40 out of 29.38

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 26.41 out of 29.38

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.90 out of 29.38

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.62 out of 27.78

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.57 out of 27.78

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.56 out of 29.38

Confidence: medium

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 26.57 out of 32.74

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 25.99 out of 30.25

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 23.21 out of 30.25

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.53 out of 28.19

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.98 out of 28.19

Confidence: medium

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 25.69 out of 32.93

Confidence: medium

Additional weak hits (6):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 25.83 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.49 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.05 out of 29.38

Confidence: weak

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 15.72 out of 18.46

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 21.58 out of 26.12

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.54 out of 30.25

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 82713 - 134462:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - Thr)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BK765_RS08495: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (31..435): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SVSLYPMSSPGGALQVGGAQQVFGMAEGLVNYTR
Nearest Stachelhaus code(s):: diOH-Bz PMPAQGVVNK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BK765_RS08505: Gly - Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (464..864): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FAMTFDISALELQALVGGETNLYGPTETTIWSTF
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1524..1937): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LATHFDFSVWEGNQVFGGEINMYGITETTVHVSY
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
ColR	Phenol-responsive regulator
FuR variant 1	Ferric Uptake Regulator
FuR variant 2	Ferric Uptake Regulator
LexA variant 3	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes
ZuR variant 1	Zinc-responsive repressor

NZ_MOOT01000040.1 - Region 2 - NRPS

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Location: 198,507 - 264,415 nt. (total: 65,909 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidase

BK765_RS08965: pyruvate oxidase/decarboxylase

smcogs: SMCOG1055: pyruvate oxidase/decarboxylase

BK765_RS09100: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BK765_RS09175: nucleotide sugar dehydrogenase

smcogs: SMCOG1152: nucleotide sugar dehydrogenase

Transferases: PPTase acetyltransferase acyltransferase aminotransferase

BK765_RS09050: Chloramphenicol acetyltransferase

smcogs: SMCOG1156: Chloramphenicol acetyltransferase

BK765_RS09060: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

BK765_RS09090: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

BK765_RS09160: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

BK765_RS09180: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

Hydrolases: deacetylase esterase

BK765_RS09010: acetylornithine deacetylase

smcogs: SMCOG1247: acetylornithine deacetylase

BK765_RS09095: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

BK765_RS09185: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Isomerases: mutase

BK765_RS08980: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: ligase

BK765_RS09075: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BK765_RS09080: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BK765_RS09085: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001133.4	0.73	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001758.4	0.73	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001133.4	0.73	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001758.4	0.73	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.03 out of 26.06

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.21 out of 26.06

Confidence: strong

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 26.19 out of 30.25

Confidence: medium

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 18.20 out of 20.01

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.02 out of 23.79

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.92 out of 27.78

Confidence: medium

Additional weak hits (5):

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.27 out of 36.53

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.58 out of 19.52

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.02 out of 26.85

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 23.97 out of 28.14

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.58 out of 19.52

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 198506 - 264415:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Thr) + (X - Ala) + (Ser - Leu - D-X - Glu)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BK765_RS09075: Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (457..866): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LSTHFDFSVWEGNQIFGGEINMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

BK765_RS09080: X - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (466..870): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: YWMTFDVSVWEWQFFSGGETNFYGPTEATIYVNA
Nearest Stachelhaus code(s):: Glu DVWHFGRINK (71% 8Å match); Gln DAWQFGLIDK (65% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1527..1919): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: MWATFDLSVFEVNTNLAGEYNLYGPSEDTTYSTY
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

BK765_RS09085: Ser - Leu - X - Glu

Search NORINE for peptide: strict or relaxed

AMP-binding (496..901): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFFTSGEINLYGPTEATVDVTY
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1558..1945): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: MWHAFDSAVQEPFAITGGDINQYGPSENTVVTTD
Nearest Stachelhaus code(s):: Pro DVQFIAQVVK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (2600..3000): X

nrpys: Gln, Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: LGLAFDASIQQMDILVGGEYNVYGPTECTVDATC
Nearest Stachelhaus code(s):: Gln or Glu DAQDLGVVDK (88% 8Å match); Ile DAQDLGVVDK (85% 8Å match); Gln DAQDLGVVDK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4115..4515): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: LGLAFDVSIQQMDMLVGGEYNVYGPTECTVDATC
Nearest Stachelhaus code(s):: Gln or Glu DAQDLGVVDK (85% 8Å match); Ile DAQDLGVVDK (82% 8Å match); Gln DAQDLGVVDK (76% 8Å match)
Stachelhaus code match:: 90% (moderate)

TFBS definitions

Regulator	Description
ArgR	Regulator of arginine biosynthesis genes
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
ColR	Phenol-responsive regulator
IolR	Regulator of the inositol catabolism
MogR	Repressor of flagellar motility genes

NZ_MOOT01000047.1 - Region 1 - terpene-precursor

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Location: 432,735 - 453,625 nt. (total: 20,891 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: carboxylase

BK765_RS14135: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

Ligases: synthase synthetase

BK765_RS14100: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

BK765_RS14105: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.39	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0002219.2	0.39	terpene	(-)-(R)-nephthenol	Stereum hirsutum FP-91666 SS1
BGC0000665.5	0.38	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0002284.3	0.38	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000639.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000642.3	0.37	terpene	carotenoid	Enterobacteriaceae bacterium DC413

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.39	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0002219.2	0.39	terpene	(-)-(R)-nephthenol	Stereum hirsutum FP-91666 SS1
BGC0000665.5	0.38	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0002284.3	0.38	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000639.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000642.3	0.37	terpene	carotenoid	Enterobacteriaceae bacterium DC413

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

BK765_RS14105: PT_FPPS_like

Domain 1 (32 - 295): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: DMAPP, IPP → FPP

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.49 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.66 out of 26.06

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 21.32 out of 25.88

Confidence: medium

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.28 out of 26.33

Confidence: medium

Additional weak hits (1):

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 25.40 out of 33.14

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
MogR	Repressor of flagellar motility genes
PsrA	Oleic acid responsive regulator

NZ_MOOT01000054.1 - Region 1 - NRPS-like

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Location: 1 - 22,169 nt. (total: 22,169 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: PPTase

BK765_RS33085: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: deaminase esterase hydrolase

BK765_RS17330: thioesterase

smcogs: SMCOG1004: thioesterase

BK765_RS17350: ornithine cyclodeaminase

smcogs: SMCOG1158: ornithine cyclodeaminase

BK765_RS17370: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Ligases: ligase synthase

BK765_RS17325: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BK765_RS17345: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.63	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.63	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002277.2	0.62	NRPS	atromentin	Suillus grevillei
BGC0001758.4	0.62	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001825.4	0.62	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002193.2	0.62	other (other)	phenguignardic acid	Aspergillus terreus NIH2624
BGC0002286.3	0.62	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.62	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.63	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.63	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002277.2	0.62	NRPS	atromentin	Suillus grevillei
BGC0001758.4	0.62	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001825.4	0.62	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002193.2	0.62	other (other)	phenguignardic acid	Aspergillus terreus NIH2624
BGC0002286.3	0.62	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.62	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.54 out of 26.06

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.75 out of 28.19

Confidence: medium

Additional weak hits (3):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.89 out of 30.25

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.62 out of 19.52

Confidence: weak

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 19.28 out of 29.51

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 22169:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asn)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BK765_RS17325: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (0..255): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWCSFDLTITGSKLVVGGEINEYGPTEAVVGCTN
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
MogR	Repressor of flagellar motility genes
ZuR variant 1	Zinc-responsive repressor

NZ_MOOT01000054.1 - Region 2 - lanthipeptide-class-i

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Location: 71,164 - 95,557 nt. (total: 24,394 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: protease

BK765_RS17660: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

BK765_RS17690: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: cyclase dehydratase

BK765_RS17695: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

BK765_RS17700: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.74	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0000560.5	0.67	ribosomal	subtilomycin	Bacillus subtilis
BGC0000507.5	0.60	ribosomal	epicidin	Staphylococcus epidermidis
BGC0000543.5	0.59	ribosomal	pep5	Staphylococcus epidermidis
BGC0000540.5	0.55	ribosomal	paenibacillin	Paenibacillus polymyxa OSY-DF
BGC0000509.4	0.52	ribosomal	epilancin 15x	Staphylococcus epidermidis
BGC0001289.5	0.52	ribosomal	penisin	Paenibacillus ehimensis
BGC0000508.3	0.47	ribosomal	epidermin	Staphylococcus epidermidis
BGC0000538.3	0.47	ribosomal	nisin Z	Lactococcus lactis
BGC0002323.2	0.46	ribosomal	nisin J	Staphylococcus capitis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.74	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0000560.5	0.67	ribosomal	subtilomycin	Bacillus subtilis
BGC0000507.5	0.60	ribosomal	epicidin	Staphylococcus epidermidis
BGC0000543.5	0.59	ribosomal	pep5	Staphylococcus epidermidis
BGC0000540.5	0.55	ribosomal	paenibacillin	Paenibacillus polymyxa OSY-DF
BGC0000509.4	0.52	ribosomal	epilancin 15x	Staphylococcus epidermidis
BGC0001289.5	0.52	ribosomal	penisin	Paenibacillus ehimensis
BGC0000508.3	0.47	ribosomal	epidermin	Staphylococcus epidermidis
BGC0000538.3	0.47	ribosomal	nisin Z	Lactococcus lactis
BGC0002323.2	0.46	ribosomal	nisin J	Staphylococcus capitis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BK765_RS17695

BK765_RS17665

Leader:

MNKELFDLDINKKMETPTEMTAQTV

Core:

PTTIFVSRSVCKTLTCICTISCSNCK

Core with dehydrations:

PDhbDhbIFVDhaRDhaVCysKDhbLDhbCysICysDhbIDhaCysDhaNCysK

antiSMASH-DB 4.0 matches for BK765_RS17665

100.0%

Lanthipeptides: BK765_RS17665_lanthipeptide

1

PTTIFVSRSVCKTLTCICTISCSNCK

26

PTTIFVSRSVCKTLTCICTISCSNCK

1

PTTIFVSRSVCKTLTCICTISCSNCK

26

96.2%

Lanthipeptides: ICC_RS25590_lanthipeptide

1

PTTIFVSRSVCKTLTCICTISCSNCK

26

PTTI VSRSVCKTLTCICTISCSNCK

1

PTTILVSRSVCKTLTCICTISCSNCK

26

70.0%

NZ_CP023711.1and 11 others:

NZ_CP034141.1 Lanthipeptides: EGM68_RS25880_lanthipeptideNZ_CP040829.1 Lanthipeptides: FGY93_RS26165_lanthipeptideNZ_CP049598.1 Lanthipeptides: G7L40_RS03920_lanthipeptideNZ_CP071875.1 Lanthipeptides: J4I02_RS05145_lanthipeptideNZ_CP086373.1 Lanthipeptides: LMH85_RS04795_lanthipeptideNZ_CP092831.1 Lanthipeptides: MLD56_RS04795_lanthipeptideNZ_CP102865.1 Lanthipeptides: NWS42_RS17805_lanthipeptideNZ_JAFJXZ010000012.1 Lanthipeptides: JYU28_RS03620_lanthipeptideNZ_JAKVDC010000009.1 Lanthipeptides: MKN04_RS18550_lanthipeptideNZ_JWJJ01000002.1 Lanthipeptides: U707_RS25810_lanthipeptideNZ_POVT01000017.1 Lanthipeptides: C1T20_RS25545_lanthipeptide

Lanthipeptides: CPY53_RS19275_lanthipeptide

1

PTTIFVSRSVCKTLTCICTISCSNCK

26

TTI VS++VCKTLTCICT SCSNCK

5

KTTIKVSKAVCKTLTCICTGSCSNCK

30

with 13 more sequence matches

Only the first 10 are displayed here.

69.2%

Lanthipeptides: DET63_RS17960_lanthipeptide

3

TIFVSRSVCKTLTCICTISCSNCK

26

TI +SR++CKTLTC+CT SCSNCK

2

TILISRTICKTLTCVCTTSCSNCK

25

65.4%

NZ_CP036100.1and 5 others:

NZ_CP036100.1 Lanthipeptides: EXW47_RS29575_lanthipeptideNZ_CP036100.1 Lanthipeptides: EXW47_RS29570_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29670_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29665_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29660_lanthipeptide

Lanthipeptides: EXW47_RS29580_lanthipeptide

2

TTIFVSRSVCKTLTCICTISCSNCK

26

T I S++ CKT TCICT SCSNCK

2

TVIKFSKAFCKTGTCICTTSCSNCK

26

64.3%

NZ_CP036100.1and 1 other:

NZ_CP072062.1 Lanthipeptides: J5V93_RS29680_lanthipeptide

Lanthipeptides: EXW47_RS29590_lanthipeptide

6

VSRSVCKTLTCICTISCSNCK

26

VSR+VCKT TCICT SCSNCK

8

VSRAVCKTGTCICTTSCSNCK

28

64.3%

NZ_MOOT01000054.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17680_lanthipeptide

Lanthipeptides: BK765_RS17685_lanthipeptide

2

TTIFVSRSVCKTLTCICTISCSNCK

26

T + VS++VCKT TCICT SCSNCK

4

TIVKVSKAVCKTGTCICTTSCSNCK

28

61.5%

NZ_KB976165.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17670_lanthipeptide

Lanthipeptides: ICC_RS25585_lanthipeptide

1

PTTIFVSRSVCKTLTCICTISCSNCK

26

T + VS+++CKT TCI TISC+NCK

1

GTVVKVSKAICKTGTCIGTISCTNCK

26

60.7%

BGC0000517.4 (BAB04173.1):

Lanthipeptides: DET63_RS17955_lanthipeptide

1

PTTIFVSRSVCKTLTCICTISCSNCK

26

T + VS+++CKT TCICT SCSNCK

3

GTVVKVSKAICKTGTCICTTSCSNCK

28

53.6%

NZ_KB976165.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17675_lanthipeptide

Lanthipeptides: ICC_RS37095_lanthipeptide

4

IFVSRSVCKTLTCICTISCSNCK

26

+ VSR+ CK+ TC+CT SCSNCK

6

LKVSRAPCKSGTCLCTTSCSNCK

28

43.8%

NZ_BDDH01000005.1and 2 others:

NZ_CP073265.1 Lanthipeptides: KCX77_RS01160_lanthipeptideNZ_JALAUD010000002.1 Lanthipeptides: MOE28_RS05435_lanthipeptide

Lanthipeptides: A7P71_RS15855_lanthipeptide

3

TIFVSRSVCKTLTCICTI-SCSNCK

26

TI S C+T TC C++ SCSNCK

8

TIVQSVKKCRTFTCGCSLGSCSNCK

32

40.6%

NC_014976.1and 6 others:

NZ_CP029461.1 Lanthipeptides: DJ572_RS00745_lanthipeptideNZ_CP031675.1 Lanthipeptides: DYS67_RS00910_lanthipeptideNZ_CP045824.1 Lanthipeptides: GII78_RS01205_lanthipeptideNZ_CP046591.1 Lanthipeptides: GO004_RS12840_lanthipeptideNZ_CP049924.1 Lanthipeptides: G4G26_RS01205_lanthipeptideNZ_CP095736.1 Lanthipeptides: MX663_RS01330_lanthipeptide

Lanthipeptides: BSN5_RS12800_lanthipeptide

3

TIFVSRSVCKTLTCICTI-SCSNC

25

TI S C+T TC C++ SCSNC

8

TIVQSVKKCRTFTCGCSLGSCSNC

31

32.4%

NZ_WIXN01000006.1and 1 other:

NZ_WIXN01000006.1 Lanthipeptides: GHD25_RS06900_lanthipeptide

Lanthipeptides: GHD25_RS06900_lanthipeptide

1

PTTIF-VSRSVCKTLTC

16

PTTI VS +VC T TC

9

PTTIIPVSLAVCPTTTC

25

MIBiG 4.0 matches for BK765_RS17665

21.4%

Lanthipeptide: haloduracin β or haloduracin α

13

TLTCICTISCS

23

TLT C SC+

18

TLTVECMPSCN

28

20.6%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

12

KTLTCICTISCS

23

KT TC C+I S

22

KTATCHCSIHVS

33

16.7%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

13

TLTCICTISCS

23

TLT C +C+

20

TLTKECQPNCN

30

with 1 more sequence matches

15.4%

BGC0001155.5 (AGY49587.1):

Thiopeptide: GE2270

13

TLTCICTISCS

23

+ C+C CS

1

SCNCVCGFCCS

11

BK765_RS17670

Leader:

MNKELFDLDINKKMEASTEMTVQTW

Core:

GTVVKVSKAICKTGTCIGTISCTNCK

Core with dehydrations:

GDhbVVKVDhaKAICysKDhbGDhbCysIGDhbIDhaCysDhbNCysK

antiSMASH-DB 4.0 matches for BK765_RS17670

100.0%

NZ_KB976165.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17670_lanthipeptide

Lanthipeptides: ICC_RS25585_lanthipeptide

1

GTVVKVSKAICKTGTCIGTISCTNCK

26

GTVVKVSKAICKTGTCIGTISCTNCK

1

GTVVKVSKAICKTGTCIGTISCTNCK

26

82.1%

Lanthipeptides: DET63_RS17955_lanthipeptide

1

GTVVKVSKAICKTGTCIGTISCTNCK

26

GTVVKVSKAICKTGTCI T SC+NCK

3

GTVVKVSKAICKTGTCICTTSCSNCK

28

73.1%

NZ_CP036100.1and 5 others:

NZ_CP036100.1 Lanthipeptides: EXW47_RS29575_lanthipeptideNZ_CP036100.1 Lanthipeptides: EXW47_RS29570_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29670_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29665_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29660_lanthipeptide

Lanthipeptides: EXW47_RS29580_lanthipeptide

2

TVVKVSKAICKTGTCIGTISCTNCK

26

TV+K SKA CKTGTCI T SC+NCK

2

TVIKFSKAFCKTGTCICTTSCSNCK

26

with 16 more sequence matches

Only the first 10 are displayed here.

71.4%

NZ_MOOT01000054.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17680_lanthipeptide

Lanthipeptides: BK765_RS17685_lanthipeptide

2

TVVKVSKAICKTGTCIGTISCTNCK

26

T+VKVSKA+CKTGTCI T SC+NCK

4

TIVKVSKAVCKTGTCICTTSCSNCK

28

64.3%

NZ_CP036100.1and 1 other:

NZ_CP072062.1 Lanthipeptides: J5V93_RS29680_lanthipeptide

Lanthipeptides: EXW47_RS29590_lanthipeptide

1

GTVVKVSKAICKTGTCIGTISCTNCK

26

G+++KVS+A+CKTGTCI T SC+NCK

3

GSILKVSRAVCKTGTCICTTSCSNCK

28

61.5%

Lanthipeptides: ICC_RS25590_lanthipeptide

1

GTVVKVSKAICKTGTCIGTISCTNCK

26

T + VS+++CKT TCI TISC+NCK

1

PTTILVSRSVCKTLTCICTISCSNCK

26

61.5%

Lanthipeptides: BK765_RS17665_lanthipeptide

1

GTVVKVSKAICKTGTCIGTISCTNCK

26

T + VS+++CKT TCI TISC+NCK

1

PTTIFVSRSVCKTLTCICTISCSNCK

26

60.0%

NZ_CP023711.1and 11 others:

NZ_CP034141.1 Lanthipeptides: EGM68_RS25880_lanthipeptideNZ_CP040829.1 Lanthipeptides: FGY93_RS26165_lanthipeptideNZ_CP049598.1 Lanthipeptides: G7L40_RS03920_lanthipeptideNZ_CP071875.1 Lanthipeptides: J4I02_RS05145_lanthipeptideNZ_CP086373.1 Lanthipeptides: LMH85_RS04795_lanthipeptideNZ_CP092831.1 Lanthipeptides: MLD56_RS04795_lanthipeptideNZ_CP102865.1 Lanthipeptides: NWS42_RS17805_lanthipeptideNZ_JAFJXZ010000012.1 Lanthipeptides: JYU28_RS03620_lanthipeptideNZ_JAKVDC010000009.1 Lanthipeptides: MKN04_RS18550_lanthipeptideNZ_JWJJ01000002.1 Lanthipeptides: U707_RS25810_lanthipeptideNZ_POVT01000017.1 Lanthipeptides: C1T20_RS25545_lanthipeptide

Lanthipeptides: CPY53_RS19275_lanthipeptide

2

TVVKVSKAICKTGTCIGTISCTNCK

26

T +KVSKA+CKT TCI T SC+NCK

6

TTIKVSKAVCKTLTCICTGSCSNCK

30

53.6%

NZ_KB976165.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17675_lanthipeptide

Lanthipeptides: ICC_RS37095_lanthipeptide

2

TVVKVSKAICKTGTCIGTISCTNCK

26

+++KVS+A CK+GTC+ T SC+NCK

4

SILKVSRAPCKSGTCLCTTSCSNCK

28

50.0%

Lanthipeptides: DET63_RS17960_lanthipeptide

4

VKVSKAICKTGTCIGTISCTNCK

26

+ +S+ ICKT TC+ T SC+NCK

3

ILISRTICKTLTCVCTTSCSNCK

25

30.8%

NZ_MWLM01000062.1:

Lassopeptides: MNBBLIKP_RS18070_lassopeptide

13

TGTCIGTISCTNCK

26

TGTC GT NC

1

TGTCQGTNGGGNCP

14

30.0%

NZ_CKMQ01000043.1and 10 others:

NZ_CKMQ01000043.1 Lanthipeptides: AE832_RS11585_lanthipeptideNZ_CKMR01000002.1 Lanthipeptides: AD782_RS11690_lanthipeptideNZ_CKMR01000002.1 Lanthipeptides: AD782_RS11690_lanthipeptideNZ_FIXU01000003.1 Lanthipeptides: A1M11_RS11670_lanthipeptideNZ_FIXU01000003.1 Lanthipeptides: A1M11_RS11670_lanthipeptideNZ_LR216041.1 Lanthipeptides: E0F00_RS05465_lanthipeptideNZ_LR216046.1 Lanthipeptides: E0E82_RS05980_lanthipeptideNZ_LR216046.1 Lanthipeptides: E0E82_RS05980_lanthipeptideNZ_PNGD01000001.1 Lanthipeptides: CJ239_RS00170_lanthipeptideNZ_PNGD01000001.1 Lanthipeptides: CJ239_RS00170_lanthipeptide

Lanthipeptides: AE832_RS11585_lanthipeptide

6

VSKAICKTGT-CIGTISCTN

24

+S+A+ G C GTI C N

8

ISEALGNKGAVCTGTIECQN

27

30.0%

NZ_JAELVP010000007.1and 1 other:

NZ_LT963438.1 Lanthipeptides: DG016_RS11145_lanthipeptide

Lanthipeptides: JF558_RS03770_lanthipeptide

1

GTVVKVSKAICKTGTCIGTISC

22

GT +K +K C T + T+SC

3

GTAIKATKNACPKVTRLVTVSC

24

BK765_RS17675

Leader:

MDKELFDLDINKKMETPTEMTAQ

Core:

TWTSILKVSRAPCKSGTCLCTTSCSNCK

Core with dehydrations:

DhbWDhbDhaILKVDhaRAPCysKDhaGDhbCysLCysDhbDhbDhaCysDhaNCysK

antiSMASH-DB 4.0 matches for BK765_RS17675

100.0%

NZ_KB976165.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17675_lanthipeptide

Lanthipeptides: ICC_RS37095_lanthipeptide

1

TWTSILKVSRAPCKSGTCLCTTSCSNCK

28

TWTSILKVSRAPCKSGTCLCTTSCSNCK

1

TWTSILKVSRAPCKSGTCLCTTSCSNCK

28

85.7%

NZ_CP036100.1and 1 other:

NZ_CP072062.1 Lanthipeptides: J5V93_RS29680_lanthipeptide

Lanthipeptides: EXW47_RS29590_lanthipeptide

1

TWTSILKVSRAPCKSGTCLCTTSCSNCK

28

TW SILKVSRA CK+GTC+CTTSCSNCK

1

TWGSILKVSRAVCKTGTCICTTSCSNCK

28

78.6%

NZ_MOOT01000054.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17680_lanthipeptide

Lanthipeptides: BK765_RS17685_lanthipeptide

1

TWTSILKVSRAPCKSGTCLCTTSCSNCK

28

TWT+I+KVS+A CK+GTC+CTTSCSNCK

1

TWTTIVKVSKAVCKTGTCICTTSCSNCK

28

with 20 more sequence matches

Only the first 10 are displayed here.

71.4%

Lanthipeptides: DET63_RS17955_lanthipeptide

1

TWTSILKVSRAPCKSGTCLCTTSCSNCK

28

TW +++KVS+A CK+GTC+CTTSCSNCK

1

TWGTVVKVSKAICKTGTCICTTSCSNCK

28

64.3%

NZ_CP036100.1and 5 others:

NZ_CP036100.1 Lanthipeptides: EXW47_RS29575_lanthipeptideNZ_CP036100.1 Lanthipeptides: EXW47_RS29570_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29670_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29665_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29660_lanthipeptide

Lanthipeptides: EXW47_RS29580_lanthipeptide

3

TSILKVSRAPCKSGTCLCTTSCSNCK

28

T+++K S+A CK+GTC+CTTSCSNCK

1

TTVIKFSKAFCKTGTCICTTSCSNCK

26

64.3%

Lanthipeptides: ICC_RS25590_lanthipeptide

3

TSILKVSRAPCKSGTCLCTTSCSNCK

28

T+IL VSR+ CK+ TC+CT SCSNCK

2

TTIL-VSRSVCKTLTCICTISCSNCK

26

53.6%

NZ_KB976165.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17670_lanthipeptide

Lanthipeptides: ICC_RS25585_lanthipeptide

4

SILKVSRAPCKSGTCLCTTSCSNCK

28

+++KVS+A CK+GTC+ T SC+NCK

2

TVVKVSKAICKTGTCIGTISCTNCK

26

53.6%

Lanthipeptides: BK765_RS17665_lanthipeptide

6

LKVSRAPCKSGTCLCTTSCSNCK

28

+ VSR+ CK+ TC+CT SCSNCK

4

IFVSRSVCKTLTCICTISCSNCK

26

53.6%

Lanthipeptides: DET63_RS17960_lanthipeptide

6

LKVSRAPCKSGTCLCTTSCSNCK

28

+ +SR CK+ TC+CTTSCSNCK

3

ILISRTICKTLTCVCTTSCSNCK

25

53.3%

NZ_CP023711.1and 11 others:

NZ_CP034141.1 Lanthipeptides: EGM68_RS25880_lanthipeptideNZ_CP040829.1 Lanthipeptides: FGY93_RS26165_lanthipeptideNZ_CP049598.1 Lanthipeptides: G7L40_RS03920_lanthipeptideNZ_CP071875.1 Lanthipeptides: J4I02_RS05145_lanthipeptideNZ_CP086373.1 Lanthipeptides: LMH85_RS04795_lanthipeptideNZ_CP092831.1 Lanthipeptides: MLD56_RS04795_lanthipeptideNZ_CP102865.1 Lanthipeptides: NWS42_RS17805_lanthipeptideNZ_JAFJXZ010000012.1 Lanthipeptides: JYU28_RS03620_lanthipeptideNZ_JAKVDC010000009.1 Lanthipeptides: MKN04_RS18550_lanthipeptideNZ_JWJJ01000002.1 Lanthipeptides: U707_RS25810_lanthipeptideNZ_POVT01000017.1 Lanthipeptides: C1T20_RS25545_lanthipeptide

Lanthipeptides: CPY53_RS19275_lanthipeptide

6

LKVSRAPCKSGTCLCTTSCSNCK

28

+KVS+A CK+ TC+CT SCSNCK

8

IKVSKAVCKTLTCICTGSCSNCK

30

46.9%

NZ_BDDH01000005.1and 2 others:

NZ_CP073265.1 Lanthipeptides: KCX77_RS01160_lanthipeptideNZ_JALAUD010000002.1 Lanthipeptides: MOE28_RS05435_lanthipeptide

Lanthipeptides: A7P71_RS15855_lanthipeptide

1

TWTSILKV---SRAPCKSGTCLCTT-SCSNCK

28

TW +I K S C++ TC C+ SCSNCK

1

TWATIGKTIVQSVKKCRTFTCGCSLGSCSNCK

32

43.8%

NC_014976.1and 6 others:

NZ_CP029461.1 Lanthipeptides: DJ572_RS00745_lanthipeptideNZ_CP031675.1 Lanthipeptides: DYS67_RS00910_lanthipeptideNZ_CP045824.1 Lanthipeptides: GII78_RS01205_lanthipeptideNZ_CP046591.1 Lanthipeptides: GO004_RS12840_lanthipeptideNZ_CP049924.1 Lanthipeptides: G4G26_RS01205_lanthipeptideNZ_CP095736.1 Lanthipeptides: MX663_RS01330_lanthipeptide

Lanthipeptides: BSN5_RS12800_lanthipeptide

1

TWTSILKV---SRAPCKSGTCLCTT-SCSNC

27

TW +I K S C++ TC C+ SCSNC

1

TWATIGKTIVQSVKKCRTFTCGCSLGSCSNC

31

32.3%

NC_010816.1and 5 others:

NZ_CP069278.1 Lanthipeptides: JQN89_RS04800_lanthipeptideNZ_JADNCJ010000029.1 Lanthipeptides: I2B64_RS09750_lanthipeptideNZ_JADNLW010000041.1 Lanthipeptides: I2F45_RS10575_lanthipeptideNZ_JADPBO010000046.1 Lanthipeptides: I4V38_RS08850_lanthipeptideNZ_JADPES010000050.1 Lanthipeptides: I4U88_RS09655_lanthipeptide

Lanthipeptides: BLD_RS08345_lanthipeptide

3

TSILKVSRAPCKSGTCLCTTSCSNCK

28

S+L VS + C SC NCK

6

VSVLSVSFSACSVTVVTRLASCGNCK

31

BK765_RS17680

Leader:

MNKELFDLDINKKMETPTEMTAQ

BK765_RS17685

Leader:

MNKELFDLDINKKMETPTEMTAQ

Shared core:

TWTTIVKVSKAVCKTGTCICTTSCSNCK

Core with dehydrations:

DhbWDhbDhbIVKVDhaKAVCysKDhbGDhbCysICysDhbDhbDhaCysDhaNCysK

antiSMASH-DB 4.0 matches for BK765_RS17680

100.0%

NZ_MOOT01000054.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17680_lanthipeptide

Lanthipeptides: BK765_RS17685_lanthipeptide

1

TWTTIVKVSKAVCKTGTCICTTSCSNCK

28

TWTTIVKVSKAVCKTGTCICTTSCSNCK

1

TWTTIVKVSKAVCKTGTCICTTSCSNCK

28

89.3%

Lanthipeptides: DET63_RS17955_lanthipeptide

1

TWTTIVKVSKAVCKTGTCICTTSCSNCK

28

TW T+VKVSKA+CKTGTCICTTSCSNCK

1

TWGTVVKVSKAICKTGTCICTTSCSNCK

28

85.7%

NZ_CP036100.1and 1 other:

NZ_CP072062.1 Lanthipeptides: J5V93_RS29680_lanthipeptide

Lanthipeptides: EXW47_RS29590_lanthipeptide

1

TWTTIVKVSKAVCKTGTCICTTSCSNCK

28

TW +I+KVS+AVCKTGTCICTTSCSNCK

1

TWGSILKVSRAVCKTGTCICTTSCSNCK

28

with 20 more sequence matches

Only the first 10 are displayed here.

78.6%

NZ_CP036100.1and 5 others:

NZ_CP036100.1 Lanthipeptides: EXW47_RS29575_lanthipeptideNZ_CP036100.1 Lanthipeptides: EXW47_RS29570_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29670_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29665_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29660_lanthipeptide

Lanthipeptides: EXW47_RS29580_lanthipeptide

3

TTIVKVSKAVCKTGTCICTTSCSNCK

28

TT++K SKA CKTGTCICTTSCSNCK

1

TTVIKFSKAFCKTGTCICTTSCSNCK

26

78.6%

NZ_KB976165.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17675_lanthipeptide

Lanthipeptides: ICC_RS37095_lanthipeptide

1

TWTTIVKVSKAVCKTGTCICTTSCSNCK

28

TWT+I+KVS+A CK+GTC+CTTSCSNCK

1

TWTSILKVSRAPCKSGTCLCTTSCSNCK

28

71.4%

NZ_KB976165.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17670_lanthipeptide

Lanthipeptides: ICC_RS25585_lanthipeptide

4

TIVKVSKAVCKTGTCICTTSCSNCK

28

T+VKVSKA+CKTGTCI T SC+NCK

2

TVVKVSKAICKTGTCIGTISCTNCK

26

70.0%

NZ_CP023711.1and 11 others:

NZ_CP034141.1 Lanthipeptides: EGM68_RS25880_lanthipeptideNZ_CP040829.1 Lanthipeptides: FGY93_RS26165_lanthipeptideNZ_CP049598.1 Lanthipeptides: G7L40_RS03920_lanthipeptideNZ_CP071875.1 Lanthipeptides: J4I02_RS05145_lanthipeptideNZ_CP086373.1 Lanthipeptides: LMH85_RS04795_lanthipeptideNZ_CP092831.1 Lanthipeptides: MLD56_RS04795_lanthipeptideNZ_CP102865.1 Lanthipeptides: NWS42_RS17805_lanthipeptideNZ_JAFJXZ010000012.1 Lanthipeptides: JYU28_RS03620_lanthipeptideNZ_JAKVDC010000009.1 Lanthipeptides: MKN04_RS18550_lanthipeptideNZ_JWJJ01000002.1 Lanthipeptides: U707_RS25810_lanthipeptideNZ_POVT01000017.1 Lanthipeptides: C1T20_RS25545_lanthipeptide

Lanthipeptides: CPY53_RS19275_lanthipeptide

4

TIVKVSKAVCKTGTCICTTSCSNCK

28

T +KVSKAVCKT TCICT SCSNCK

6

TTIKVSKAVCKTLTCICTGSCSNCK

30

64.3%

Lanthipeptides: ICC_RS25590_lanthipeptide

4

TIVKVSKAVCKTGTCICTTSCSNCK

28

T + VS++VCKT TCICT SCSNCK

2

TTILVSRSVCKTLTCICTISCSNCK

26

64.3%

Lanthipeptides: BK765_RS17665_lanthipeptide

4

TIVKVSKAVCKTGTCICTTSCSNCK

28

T + VS++VCKT TCICT SCSNCK

2

TTIFVSRSVCKTLTCICTISCSNCK

26

53.6%

BGC0000535.4 (ADJ56352.1):

Lanthipeptides: DET63_RS17960_lanthipeptide

6

VKVSKAVCKTGTCICTTSCSNCK

28

+ +S+ +CKT TC+CTTSCSNCK

3

ILISRTICKTLTCVCTTSCSNCK

25

53.1%

NZ_BDDH01000005.1and 2 others:

NZ_CP073265.1 Lanthipeptides: KCX77_RS01160_lanthipeptideNZ_JALAUD010000002.1 Lanthipeptides: MOE28_RS05435_lanthipeptide

Lanthipeptides: A7P71_RS15855_lanthipeptide

1

TWTTIVKV---SKAVCKTGTCICTT-SCSNCK

28

TW TI K S C+T TC C+ SCSNCK

1

TWATIGKTIVQSVKKCRTFTCGCSLGSCSNCK

32

50.0%

NC_014976.1and 6 others:

NZ_CP029461.1 Lanthipeptides: DJ572_RS00745_lanthipeptideNZ_CP031675.1 Lanthipeptides: DYS67_RS00910_lanthipeptideNZ_CP045824.1 Lanthipeptides: GII78_RS01205_lanthipeptideNZ_CP046591.1 Lanthipeptides: GO004_RS12840_lanthipeptideNZ_CP049924.1 Lanthipeptides: G4G26_RS01205_lanthipeptideNZ_CP095736.1 Lanthipeptides: MX663_RS01330_lanthipeptide

Lanthipeptides: BSN5_RS12800_lanthipeptide

1

TWTTIVKV---SKAVCKTGTCICTT-SCSNCK

28

TW TI K S C+T TC C+ SCSNC

1

TWATIGKTIVQSVKKCRTFTCGCSLGSCSNCN

32

38.7%

NZ_MLKL01000042.1:

Lanthipeptides: BKK45_RS27405_lanthipeptide

4

TIVKVSKAVCKTGTCICTTSC

24

TIV VS +C TG +CT SC

13

TIVAVSVKLCPTG--VCTKSC

31

MIBiG 4.0 matches for BK765_RS17680

11.8%

Lanthipeptide: nisin A

11

AVCKTG

16

CKTG

9

PGCKTG

14

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 24.01 out of 30.25

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.06 out of 29.38

Confidence: medium

Additional weak hits (6):

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 24.41 out of 30.97

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 24.71 out of 31.73

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 25.92 out of 31.73

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 25.20 out of 32.74

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.87 out of 30.98

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.87 out of 30.98

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

BK765_RS17665 - Class I

Cleavage pHMM score: 10.10

RODEO score: 26

Monoisotopic mass: 2645.4 Da

Molecular weight: 2647.2 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 2665.2 Da N = 1; 2683.2 Da N = 2; 2701.3 Da N = 3; 2719.3 Da N = 4

BK765_RS17670 - Class I

Cleavage pHMM score: 9.90

RODEO score: 24

Monoisotopic mass: 2488.3 Da

Molecular weight: 2490.0 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 2508.0 Da N = 1; 2526.1 Da N = 2; 2544.1 Da N = 3

BK765_RS17675 - Class I

Cleavage pHMM score: 10.10

RODEO score: 26

Monoisotopic mass: 2797.4 Da

Molecular weight: 2799.3 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 2817.3 Da N = 1; 2835.3 Da N = 2; 2853.4 Da N = 3; 2871.4 Da N = 4; 2889.4 Da N = 5

BK765_RS17680 - Class I

Cleavage pHMM score: 10.10

RODEO score: 26

Monoisotopic mass: 2785.4 Da

Molecular weight: 2787.3 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 2805.3 Da N = 1; 2823.4 Da N = 2; 2841.4 Da N = 3; 2859.4 Da N = 4; 2877.4 Da N = 5

BK765_RS17685 - Class I

Cleavage pHMM score: 10.10

RODEO score: 26

Monoisotopic mass: 2785.4 Da

Molecular weight: 2787.3 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 2805.3 Da N = 1; 2823.4 Da N = 2; 2841.4 Da N = 3; 2859.4 Da N = 4; 2877.4 Da N = 5

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 147 - 238; Score: 58.9

TFBS definitions

Regulator	Description
ColR	Phenol-responsive regulator
FuR variant 1	Ferric Uptake Regulator

NZ_MOOT01000057.1 - Region 1 - NRPS,T1PKS,lanthipeptide-class-ii

Download region SVG

Location: 57,991 - 167,674 nt. (total: 109,684 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

BK765_RS20055: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BK765_RS20065: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

BK765_RS20070: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BK765_RS20085: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

BK765_RS20140: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

BK765_RS20155: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

BK765_RS20165: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

BK765_RS20180: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

BK765_RS20230: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Transferases: PPTase acylase aminotransferase carbamoyltransferase

BK765_RS20150: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BK765_RS20210: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

BK765_RS20220: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

BK765_RS20240: ornithine carbamoyltransferase

smcogs: SMCOG1114: ornithine carbamoyltransferase

Hydrolases: deaminase esterase hydrolase phosphatase

BK765_RS20185: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

BK765_RS20190: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

BK765_RS20200: thioesterase

smcogs: SMCOG1004: thioesterase

BK765_RS20250: ornithine cyclodeaminase

smcogs: SMCOG1158: ornithine cyclodeaminase

Lyases: cyclase decarboxylase

BK765_RS20080: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

BK765_RS20270: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Ligases: ligase synthase

BK765_RS20090: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BK765_RS20120: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BK765_RS20125: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BK765_RS20130: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BK765_RS20170: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BK765_RS20175: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BK765_RS20195: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BK765_RS20205: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BK765_RS20245: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001059.5	1.51	NRPS, PKS	zwittermicin A	Bacillus cereus
BGC0000626.3	1.44	ribosomal	thuricin	Bacillus thuringiensis
BGC0001833.4	0.94	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.90	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.89	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.89	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.89	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.89	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002075.3	0.86	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001825.4	0.84	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001059.5	0.94	NRPS, PKS	zwittermicin A	Bacillus cereus
BGC0000626.3	0.90	ribosomal	thuricin	Bacillus thuringiensis
BGC0001833.4	0.77	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.75	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.75	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.75	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.75	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001825.4	0.73	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

Similar subclusters

Lanthipeptide predictions

Lanthipeptide(s) for BK765_RS20270

BK765_RS20265

Leader:

METEKYLQVVEDEEIEQLVGG

Core:

VGPGWIATLTKDCPANKPYACITIAGQTICKKCR

Core with dehydrations:

VGPGWIADhbLDhbKDCysPANKPYACysIDhbIAGQDhbICysKKCysR

antiSMASH-DB 4.0 matches for BK765_RS20265

100.0%

NZ_CM000758.1and 1 other:

NZ_MOOT01000057.1 Lanthipeptides: BK765_RS20265_lanthipeptide

Lanthipeptides: BTHUR0013_RS15850_lanthipeptide

1

VGPGWIATLTKDCPANKPYACITIAGQTICKKCR

34

VGPGWIATLTKDCPANKPYACITIAGQTICKKCR

1

VGPGWIATLTKDCPANKPYACITIAGQTICKKCR

34

85.3%

BGC0000582.4 (ADR72962.1):

Lanthipeptides: ICC_RS24850_lanthipeptide

1

VGPGWIATLTKDCPANKPYACITIAGQTICKKCR

34

+GPGW+ TLTKDCP KPYACITIAGQTICKKCR

1

IGPGWVETLTKDCPGYKPYACITIAGQTICKKCR

34

73.5%

NZ_CM000748.1and 1 other:

NZ_JAIPUK010000011.1 Lanthipeptides: K9P71_RS26815_lanthipeptide

Lanthipeptides: BTHUR0003_RS30910_lanthipeptide

1

VGPGWIATLTKDCPANKPYACITIAGQTICKKCR

34

+GPGW+ TLTKDCP N P AC+TI GQ ICKKCR

1

IGPGWVETLTKDCPWNMPVACVTIMGQRICKKCR

34

with 55 more sequence matches

Only the first 10 are displayed here.

70.6%

NZ_JH792312.1and 1 other:

NZ_QNRN01000010.1 Lanthipeptides: DET63_RS17570_lanthipeptide

Lanthipeptides: IIG_RS24665_lanthipeptide

2

GPGWIATLTKDCPANKPYACITIAGQTICKKC

33

GPGW+ TLTKDCP N P AC+TI GQ ICKKC

2

GPGWVETLTKDCPWNVPVACVTIMGQRICKKC

33

67.6%

NZ_CP036100.1and 1 other:

NZ_CP072062.1 Lanthipeptides: J5V93_RS29280_lanthipeptide

Lanthipeptides: EXW47_RS29185_lanthipeptide

2

GPGWIATLTKDCPANKPYACITIAGQTICKKC

33

GPGW+ T+TKDCP N P AC+TI GQ ICKKC

2

GPGWVETVTKDCPWNVPVACVTIMGQRICKKC

33

64.7%

NZ_CM000731.1and 2 others:

NZ_CP043834.1 Lanthipeptides: F0362_RS30375_lanthipeptideNZ_NVOB01000050.1 Lanthipeptides: CON62_RS29235_lanthipeptide

Lanthipeptides: BCERE0020_RS28875_lanthipeptide

2

GPGWIATLTKDCPANKPYACITIAGQTICKKC

33

GPGW+ TLTKDCP +P AC+ I GQ ICKKC

2

GPGWVETLTKDCPWKQPAACVIIMGQKICKKC

33

64.7%

NZ_CP036053.1and 7 others:

NZ_JAOPRA010000024.1 Lanthipeptides: OCE16_RS27090_lanthipeptideNZ_KB976667.1 Lanthipeptides: IG5_RS06165_lanthipeptideNZ_LDKD02000003.1 Lanthipeptides: ABH17_RS27060_lanthipeptideNZ_NUAO01000019.1 Lanthipeptides: CN544_RS19345_lanthipeptideNZ_NVFH01000027.1 Lanthipeptides: COL40_RS24675_lanthipeptideNZ_NVHY01000021.1 Lanthipeptides: COM05_RS26660_lanthipeptideNZ_NVOV01000033.1 Lanthipeptides: CON78_RS26470_lanthipeptide

Lanthipeptides: EXW64_RS27135_lanthipeptide

2

GPGWIATLTKDCPANKPYACITIAGQTICKKC

33

GPGW+ TLTKDCP +P AC+ I GQ ICKKC

2

GPGWVETLTKDCPWKQPGACVIIMGQKICKKC

33

64.7%

NZ_KB976276.1:

Lanthipeptides: IIC_RS09105_lanthipeptide

2

GPGWIATLTKDCPANKPYACITIAGQTICKKC

33

GPGW+ TLTKDCP +P AC+ I GQ ICKKC

2

GPGWVETLTKDCPWKQPVACVIIMGQKICKKC

33

52.9%

NZ_FNYB01000007.1and 1 other:

NZ_KB976303.1 Lanthipeptides: IGY_RS28100_lanthipeptide

Lanthipeptides: BMZ34_RS15340_lanthipeptide

1

VGPGWIATLTKDCPANKPYACITIAGQTICKKC

33

+GPG+I TLTKDCP P C+ I G CK+C

1

IGPGYIETLTKDCPRVTPQICVPINGIWTCKRC

33

50.0%

NZ_CP040409.1and 3 others:

NZ_CP040415.1 Lanthipeptides: FE330_RS03440_lanthipeptideNZ_CP040417.1 Lanthipeptides: FE328_RS00755_lanthipeptideNZ_CP040424.1 Lanthipeptides: FE321_RS03455_lanthipeptide

Lanthipeptides: FE336_RS03460_lanthipeptide

1

VGPGWIATLTKDCPANKPYACITIAGQTICKKCR

34

VGPG + TLTKDCP P C CK CR

1

VGPGLVRTLTKDCPNVVPKVCF----DGNCKYCR

30

50.0%

NZ_CP040414.1:

Lanthipeptides: FE331_RS04030_lanthipeptide

1

VGPGWIATLTKDCPANKPYACITIAGQTICKKCR

34

VGPG + TLTKDCP P C CK CR

1

VGPGLVRTLTKDCPNVVPKVCF----DGKCKYCR

30

47.1%

NZ_CP040409.1and 4 others:

NZ_CP040414.1 Lanthipeptides: FE331_RS04020_lanthipeptideNZ_CP040415.1 Lanthipeptides: allorf_0721024_0721203_lanthipeptideNZ_CP040417.1 Lanthipeptides: FE328_RS00745_lanthipeptideNZ_CP040424.1 Lanthipeptides: FE321_RS03445_lanthipeptide

Lanthipeptides: FE336_RS03450_lanthipeptide

2

GPGWIATLTKDCPANKPYACITIAGQTICKKCR

34

GPG + TLTKDCP C+ +A + CKKC

2

GPGLLKTLTKDCPGTVSDVCVNLAVVSTCKKCE

34

41.2%

NC_017621.1and 16 others:

NZ_CP039462.1 Lanthipeptides: E8M06_RS05390_lanthipeptideNZ_JABTZM010000032.1 Lanthipeptides: HRQ47_RS09675_lanthipeptideNZ_JAEVFQ010000010.1 Lanthipeptides: JNO64_RS06375_lanthipeptideNZ_JAFFHD010000003.1 Lanthipeptides: JN532_RS02075_lanthipeptideNZ_JAGFVG010000007.1 Lanthipeptides: J5581_RS04225_lanthipeptideNZ_JAIJZO010000001.1 Lanthipeptides: K5Q34_RS00965_lanthipeptideNZ_JAIMDS010000024.1 Lanthipeptides: K6V32_RS10650_lanthipeptideNZ_JAJUWV010000001.1 Lanthipeptides: LZF89_RS05505_lanthipeptideNZ_JAMDIS010000017.1 Lanthipeptides: M5P45_RS08180_lanthipeptideNZ_JAMYIU010000001.1 Lanthipeptides: NKE44_RS01780_lanthipeptideNZ_JAMYIX010000001.1 Lanthipeptides: NKE54_RS02675_lanthipeptideNZ_JAMYIY010000001.1 Lanthipeptides: NKE64_RS02785_lanthipeptideNZ_JAMYJP010000014.1 Lanthipeptides: NKE68_RS07350_lanthipeptideNZ_LR738722.1 Lanthipeptides: STREPSUIS_RS03955_lanthipeptideNZ_VZMP01000023.1 Lanthipeptides: F6P50_RS09295_lanthipeptideNZ_VZNS01000007.1 Lanthipeptides: F6P79_RS05700_lanthipeptide

Lanthipeptides: SSUD12_RS06540_lanthipeptide

2

GPGWIATLTKDCPANKPYACITIAGQTICKKC

33

G G++ TLTKDCP C+ I + CK C

2

GSGFVKTLTKDCPGFLSNVCVNIGFISGCKNC

33

MIBiG 4.0 matches for BK765_RS20265

26.5%

Linaridin: cypemycin

14

PANKPYACITIAGQTICKKC

33

PA A I G TIC C

3

PATPTVAQFVIQGSTICLVC

22

23.5%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

4

GWIATLTKDCPAN

16

GW TLTK+C N

17

GW-CTLTKECQPN

28

BK765_RS32535

Leader:

MEKTKSKLNNAAIEGVIAA

Core:

LKDENECLRMEIDYLKKLKALVQKEKKLQSFPNEKAHRL

Core with dehydrations:

LKDENECysLRMEIDYLKKLKALVQKEKKLQDhaFPNEKAHRL

antiSMASH-DB 4.0 matches for BK765_RS32535

100.0%

NZ_MOOT01000057.1:

Lanthipeptides: BK765_RS32535_lanthipeptide

1

LKDENECLRMEIDYLKKLKALVQKEKKLQSFPNEKAHRL

39

LKDENECLRMEIDYLKKLKALVQKEKKLQSFPNEKAHRL

1

LKDENECLRMEIDYLKKLKALVQKEKKLQSFPNEKAHRL

39

35.9%

NZ_CM000758.1:

Lanthipeptides: BTHUR0013_RS36715_lanthipeptide

1

LKDENECLRMEIDY

14

LKDENECLRMEIDY

4

LKDENECLRMEIDY

17

33.3%

NZ_CM000748.1and 1 other:

NZ_KB976165.1 Lanthipeptides: ICC_RS35950_lanthipeptide

Lanthipeptides: BTHUR0003_RS35340_lanthipeptide

1

LKDENECLRMEIDY

14

LKDENECLRMEI+Y

4

LKDENECLRMEINY

17

with 2 more sequence matches

23.1%

NZ_CP063161.1:

Lanthipeptides: allorf_46040_46171_lanthipeptide

10

MEIDYLKKLKALVQKEKKLQSFPNEKAH

37

M+I YL + LV K + S ++ H

2

MQIQYLVLMIPLVVKSLNISSLDSKFIH

29

10.2%

NZ_KB944819.1:

Lanthipeptides: WOK_RS14385_lanthipeptide

28

LQSFPNEKAHRL

39

++SFP E+ H++

11

VKSFPEEEGHQV

22

BK765_RS20310

Leader:

MKKKRDFLFKFVSSLVCLIFILQTSSVSFA

Core:

ESSSEPLAPKHVNSCDKLLESISVEEIERTVSLENNTETSELPAI

Core with dehydrations:

EDhaDhaDhaEPLAPKHVNDhaCysDKLLEDhaIDhaVEEIERDhbVDhaLENNDhbEDhbDhaELPAI

antiSMASH-DB 4.0 matches for BK765_RS20310

100.0%

NZ_MOOT01000057.1:

Lanthipeptides: BK765_RS20310_lanthipeptide

1

ESSSEPLAPKHVNSCDKLLESISVEEIERTVSLENNTETSELPAI

45

ESSSEPLAPKHVNSCDKLLESISVEEIERTVSLENNTETSELPAI

1

ESSSEPLAPKHVNSCDKLLESISVEEIERTVSLENNTETSELPAI

45

97.8%

NZ_JAIPUK010000011.1:

Lanthipeptides: K9P71_RS26765_lanthipeptide

1

ESSSEPLAPKHVNSCDKLLESISVEEIERTVSLENNTETSELPAI

45

ESSSEPLAPKHVNSCDKLL+SISVEEIERTVSLENNTETSELPAI

1

ESSSEPLAPKHVNSCDKLLDSISVEEIERTVSLENNTETSELPAI

45

17.8%

NZ_CP060140.1:

Lanthipeptides: allorf_1942151_1942312_lanthipeptide

11

HVNSCDKLLESISVEEIERTVSLENNTET

39

+ ++C K+ S V EI + + NT +

6

YFDNCSKVWRSSGVREIMYFLHIWQNTWS

34

allorf_154067_154177

Leader:

MFLTRFLQFKIDTRSLQRLLGS

Core:

VNSVREFKCIKVIS

Core with dehydrations:

VNDhaVREFKCysIKVIDha

antiSMASH-DB 4.0 matches for allorf_154067_154177

100.0%

NZ_CM000758.1and 1 other:

NZ_MOOT01000057.1 Lanthipeptides: allorf_154067_154177_lanthipeptide

Lanthipeptides: allorf_3252373_3252483_lanthipeptide

1

VNSVREFKCIKVIS

14

VNSVREFKCIKVIS

1

VNSVREFKCIKVIS

14

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 25.18 out of 26.06

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.88 out of 23.79

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 23.21 out of 30.25

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.55 out of 19.26

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 23.21 out of 30.25

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.23 out of 27.78

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.17 out of 29.38

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.81 out of 28.19

Confidence: medium

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 25.01 out of 32.93

Confidence: medium

Additional weak hits (8):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 24.19 out of 28.14

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.72 out of 32.74

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.60 out of 30.98

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.90 out of 20.01

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.94 out of 20.01

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 24.48 out of 31.73

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 24.92 out of 31.73

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 21.58 out of 26.12

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Core peptide predictions

BK765_RS20265 - Class II

Cleavage pHMM score: -0.80

RODEO score: 0

Monoisotopic mass: 3547.8 Da

Molecular weight: 3550.2 Da

Number of bridges: 4

BK765_RS32535 - Class II

Cleavage pHMM score: -6.60

RODEO score: 0

Monoisotopic mass: 4707.6 Da

Molecular weight: 4710.5 Da

Number of bridges: 1

BK765_RS20310 - Class II

Cleavage pHMM score: -7.30

RODEO score: 0

Monoisotopic mass: 4725.4 Da

Molecular weight: 4728.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4746.1 Da N = 1; 4764.2 Da N = 2; 4782.2 Da N = 3; 4800.2 Da N = 4; 4818.2 Da N = 5; 4836.2 Da N = 6; 4854.3 Da N = 7; 4872.3 Da N = 8; 4890.3 Da N = 9; 4908.3 Da N = 10

allorf_154067_154177 - Class II

Cleavage pHMM score: -5.10

RODEO score: 0

Monoisotopic mass: 1584.9 Da

Molecular weight: 1585.9 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1603.9 Da N = 1

Predicted core structure(s)

For candidate cluster 1, location 57990 - 167674:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Tyr) + (ohmal - bOH-Tyr) + (Pro) + (X - ohmal) + (D-Asn) + (X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 57990 - 162523:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Tyr) + (ohmal - bOH-Tyr) + (Pro) + (X - ohmal) + (D-Asn) + (X - X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BK765_RS20090: X - Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (479..879): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LSVAFDASIMEPVLFVGGELNAYGPTETTVCATM
Nearest Stachelhaus code(s):: Leu DAMLVGAVCK (71% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1542..1946): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: LWRYFDSSVSESDLIMGGEVNEYGPTEATVSAMY
Nearest Stachelhaus code(s):: Lys DAEDIGTVSK (65% 8Å match)
Stachelhaus code match:: 70% (weak)

BK765_RS20120: mal

PKS_AT (1893..2187): mal

ATSignature: 2-Rhyd-Malonyl-CoA

Top 3 matches:: 2-Rhyd-Malonyl-CoA: 100.0%; Malonyl-CoA: 62.5%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 107.0
inactive: 74.6
Methoxymalonyl-CoA: 50.9
Methylmalonyl-CoA: 41.7
Propionyl-CoA: 29.7
Ethylmalonyl-CoA: 14.1
2-Methylbutyryl-CoA: 13.3
CHC-CoA: 12.9
3-Methylbutyryl-CoA: 10.8
Isobutyryl-CoA: 9.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0

BK765_RS20125: bOH-Tyr - Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (274..685): bOH-Tyr

nrpys: bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LGLSFDASVQNGDGIIAGETNAYGPTETTVDSTC
Nearest Stachelhaus code(s):: bOH-Tyr DASTIAAVDK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1329..1728): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: FSMTFDVSVLEGNFLLAGEENMYGPTEATVIAST
Nearest Stachelhaus code(s):: Ala DVHNLAIVYK (76% 8Å match); Ser DVWNVAMVHK (62% 8Å match)
Stachelhaus code match:: 70% (weak)

BK765_RS20150: mal

PKS_AT (5..291): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 51.2
inactive: 31.6
Methoxymalonyl-CoA: 30.9
Methylmalonyl-CoA: 13.9
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

BK765_RS20170: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (8..410): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFFVSGETNLYGPTEATIDVTA
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

BK765_RS20175: X - mal

AMP-binding (448..845): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LHQHFDMSVWEGNQTFGGEVNMYGITETTVHVTY
Nearest Stachelhaus code(s):: Ser DMWNTGLVHK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

PKS_AT (1551..1847): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 130.8
inactive: 98.3
Methoxymalonyl-CoA: 43.7
Methylmalonyl-CoA: 24.3
Propionyl-CoA: 16.0
Isobutyryl-CoA: 8.9
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

BK765_RS20195: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (461..856): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWASFDLTVTSTKVIVGGEINEYGPTETVVGCMI
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BK765_RS20205: X - X

AMP-binding (280..682): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAMSFDVTIFEVYIFVGVELNLYGPTEATVCCTS
Nearest Stachelhaus code(s):: Met DVFYLGGVFK (59% 8Å match); Ala DVFFVGLVAK (50% 8Å match); Pro DLFYLALVCK (47% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1323..1725): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAMSFDVTIFEVYILVGVELNGYGPTEATVCCTT
Nearest Stachelhaus code(s):: Met DVFYLGGVFK (62% 8Å match)
Stachelhaus code match:: 90% (moderate)

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
ColR	Phenol-responsive regulator
FuR variant 1	Ferric Uptake Regulator
IolR	Regulator of the inositol catabolism
LexA variant 3	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes
ZuR variant 1	Zinc-responsive repressor

NZ_MOOT01000059.1 - Region 1 - RiPP-like

Download region SVG

Location: 86,115 - 96,378 nt. (total: 10,264 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

BK765_RS21080: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Similar gene clusters

Analysis type:

Reference	RiPP-like	Similarity score	Type	Compound(s)	Organism
BGC0001994.4		0.35	other (other)	β-ethynylserine	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0002373.3		0.19	NRPS	bonnevillamide D, bonnevillamide E	Streptomyces sp.

Reference	Aggregated	Similarity score	Type	Compound(s)	Organism
BGC0001994.4		0.35	other (other)	β-ethynylserine	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0002373.3		0.19	NRPS	bonnevillamide D, bonnevillamide E	Streptomyces sp.

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.32 out of 26.06

Confidence: strong

Additional weak hits (2):

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 24.59 out of 35.63

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.40 out of 36.53

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
MogR	Repressor of flagellar motility genes

NZ_MOOT01000059.1 - Region 2 - NRPS

Download region SVG

Location: 115,620 - 162,636 nt. (total: 47,017 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

BK765_RS21255: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

BK765_RS21385: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: aminotransferase glycosyltransferase

BK765_RS21240: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

BK765_RS21380: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: esterase glucanase hydrolase

BK765_RS21320: alpha/beta hydrolase domain-containing protein

smcogs: SMCOG1066: alpha/beta hydrolase domain-containing protein

BK765_RS21340: Pullulanase, type I

smcogs: SMCOG1297: Pullulanase, type I

BK765_RS21350: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase

smcogs: SMCOG1283: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase

BK765_RS21390: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: decarboxylase

BK765_RS21325: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Ligases: ligase

BK765_RS21330: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.72	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.64	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0002286.3	0.63	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000357.5	0.62	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001641.4	0.62	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.72	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.64	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0002286.3	0.63	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000357.5	0.62	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001641.4	0.62	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.93 out of 30.25

Confidence: medium

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 24.13 out of 29.25

Confidence: medium

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 28.23 out of 31.73

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.58 out of 20.97

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.59 out of 23.05

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.66 out of 25.88

Confidence: medium

Additional weak hits (5):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.02 out of 32.74

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 24.69 out of 31.73

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 24.46 out of 31.73

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 23.36 out of 33.73

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.63 out of 29.65

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 115619 - 162636:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Cys)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BK765_RS21330: X - Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (268..658): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FVMAFDISLLELESLLAGELNIYGPTETTICATV
Nearest Stachelhaus code(s):: Ala DILICALICK (44% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1305..1685): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TDISFDLSVYDGNTLHSGDVSLGGATEASIWSIY
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CcpA	Catabolite control protein A
ColR	Phenol-responsive regulator
HexR	Glucose-responsive regulator
PsrA	Oleic acid responsive regulator
SypG	Regulator of biofilm formation and host colonization

NZ_MOOT01000060.1 - Region 1 - RiPP-like

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Location: 10,419 - 20,748 nt. (total: 10,330 nt) Show pHMM detection rules used

Region on contig edge.

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase

BK765_RS23150: aminotransferase

smcogs: SMCOG1019: aminotransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000896.5	0.29	other (other)	cycloserine	Streptomyces lavendulae subsp. lavendulae
BGC0000721.4	0.26	saccharide	tobramycin	Streptoalloteichus tenebrarius
BGC0000381.5	0.23	NRPS	lichenysin	Bacillus licheniformis DSM 13 = ATCC 14580
BGC0000172.5	0.23	PKS	Basiliskamide A, Basiliskamide B	Brevibacillus laterosporus PE36
BGC0000433.5	0.22	NRPS	surfactin	Bacillus velezensis FZB42
BGC0000455.6	0.22	NRPS, saccharide	vancomycin	Amycolatopsis orientalis HCCB10007 (ATCC43491)
BGC0002588.2	0.22	other (other)	enduracididine	uncultured bacterium
BGC0001233.5	0.22	NRPS	feglymycin	Streptomyces sp. DSM 11171
BGC0001461.5	0.21	NRPS	Actinoidin B	Amycolatopsis keratiniphila subsp. nogabecina
BGC0001459.5	0.21	NRPS	decaplanin	Amycolatopsis regifaucium

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000896.5	0.29	other (other)	cycloserine	Streptomyces lavendulae subsp. lavendulae
BGC0000721.4	0.26	saccharide	tobramycin	Streptoalloteichus tenebrarius
BGC0000381.5	0.23	NRPS	lichenysin	Bacillus licheniformis DSM 13 = ATCC 14580
BGC0000172.5	0.23	PKS	Basiliskamide A, Basiliskamide B	Brevibacillus laterosporus PE36
BGC0000433.5	0.22	NRPS	surfactin	Bacillus velezensis FZB42
BGC0000455.6	0.22	NRPS, saccharide	vancomycin	Amycolatopsis orientalis HCCB10007 (ATCC43491)
BGC0002588.2	0.22	other (other)	enduracididine	uncultured bacterium
BGC0001233.5	0.22	NRPS	feglymycin	Streptomyces sp. DSM 11171
BGC0001461.5	0.21	NRPS	Actinoidin B	Amycolatopsis keratiniphila subsp. nogabecina
BGC0001459.5	0.21	NRPS	decaplanin	Amycolatopsis regifaucium

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.30 out of 26.06

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.22 out of 24.78

Confidence: medium

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 24.87 out of 32.93

Confidence: medium

Additional weak hits (2):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.27 out of 25.75

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 17.35 out of 30.12

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
LexA variant 2	Repressor of DNA damage response
LexA variant 3	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes

NZ_MOOT01000060.1 - Region 2 - betalactone

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Location: 71,570 - 96,808 nt. (total: 25,239 nt) Show pHMM detection rules used

betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BK765_RS23480: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: acetyltransferase carboxylase transferase

BK765_RS23435: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

BK765_RS23455: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

BK765_RS23475: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

Lyases: hydratase

BK765_RS23460: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase synthase

BK765_RS23450: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BK765_RS23465: 2-isopropylmalate synthase

smcogs: SMCOG1271: 2-isopropylmalate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002284.3	0.55	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000131.5	0.46	PKS	pyrrolomycin	Streptomyces sp. UC 11065
BGC0000706.3	0.46	saccharide	kanamycin	Streptomyces kanamyceticus
BGC0000234.5	0.43	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0000623.3	0.42	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0001103.5	0.42	NRPS, PKS	mycosubtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0001226.4	0.42	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000563.4	0.42	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000558.5	0.42	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0002700.2	0.41	ribosomal	bacinapeptin	Bacillus nakamurai

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002284.3	0.55	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000131.5	0.46	PKS	pyrrolomycin	Streptomyces sp. UC 11065
BGC0000706.3	0.46	saccharide	kanamycin	Streptomyces kanamyceticus
BGC0000234.5	0.43	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0000623.3	0.42	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0001103.5	0.42	NRPS, PKS	mycosubtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0001226.4	0.42	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000563.4	0.42	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000558.5	0.42	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0002700.2	0.41	ribosomal	bacinapeptin	Bacillus nakamurai

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.06 out of 19.26

Confidence: strong

Additional weak hits (2):

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 17.04 out of 30.12

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 18.69 out of 30.12

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein

NZ_MOOT01000061.1 - Region 1 - lanthipeptide-class-ii,terpene-precursor

BGC0001229.4 (SBI_06989):

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Location: 63,035 - 81,613 nt. (total: 18,579 nt) Show pHMM detection rules used

Region on contig edge.

terpene-precursor: (PT_FPPS_like and not PT_HPP)
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: protease

BK765_RS23950: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: cyclase

BK765_RS23980: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000502.5	1.29	ribosomal	cerecidin, cerecidin A1, cerecidin A2, cerecidin A3, cerecidin A4, cerecidin A5, cerecidin A6, cerecidin A7	Bacillus cereus
BGC0000504.5	0.55	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000554.4	0.45	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000500.5	0.42	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0001210.5	0.41	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0001555.4	0.39	ribosomal	colicin V	Escherichia coli chi7122
BGC0000517.4	0.39	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0000590.5	0.38	ribosomal	microcin N	Escherichia coli
BGC0000525.5	0.37	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0000527.5	0.37	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000502.5	0.83	ribosomal	cerecidin, cerecidin A1, cerecidin A2, cerecidin A3, cerecidin A4, cerecidin A5, cerecidin A6, cerecidin A7	Bacillus cereus
BGC0000504.5	0.62	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000554.4	0.56	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000500.5	0.54	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0001210.5	0.53	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000517.4	0.52	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0001555.4	0.52	ribosomal	colicin V	Escherichia coli chi7122
BGC0000590.5	0.51	ribosomal	microcin N	Escherichia coli
BGC0000527.5	0.51	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BK765_RS23980

BK765_RS23985

Leader:

MSKEYKFTKEELVEAWKDPQVREKLKDLPKHPSGKALNELSEEELAEIQGA

Core:

SDVQPETTPLCVGVIIGITASIKICK

Core with dehydrations:

DhaDVQPEDhbDhbPLCysVGVIIGIDhbADhaIKICysK

antiSMASH-DB 4.0 matches for BK765_RS23985

100.0%

NZ_CP067069.1and 13 others:

NZ_CP085413.1 Lanthipeptides: LIS80_RS25350_lanthipeptideNZ_JH792105.1 Lanthipeptides: II5_RS26960_lanthipeptideNZ_JH792240.1 Lanthipeptides: IIK_RS26240_lanthipeptideNZ_MOOT01000061.1 Lanthipeptides: BK765_RS23985_lanthipeptideNZ_NTUF01000024.1 Lanthipeptides: CN379_RS26680_lanthipeptideNZ_NVCZ01000001.1 Lanthipeptides: COK75_RS01180_lanthipeptideNZ_NVDK01000008.1 Lanthipeptides: COK95_RS05665_lanthipeptideNZ_NVEA01000001.1 Lanthipeptides: COL15_RS00010_lanthipeptideNZ_PIYQ01000022.1 Lanthipeptides: CW365_RS08190_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20675_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19560_lanthipeptideNZ_WBPE01000025.1 Lanthipeptides: F8161_RS14435_lanthipeptideNZ_WBPK01000016.1 Lanthipeptides: F8167_RS15685_lanthipeptide

Lanthipeptides: JCR32_RS24620_lanthipeptide

1

SDVQPETTPLCVGVIIGITASIKICK

26

SDVQPETTPLCVGVIIGITASIKICK

1

SDVQPETTPLCVGVIIGITASIKICK

26

96.2%

NZ_LDKE01000067.1:

Lanthipeptides: ABB04_RS19960_lanthipeptide

1

SDVQPETTPLCVGVIIGITASIKICK

26

SDV+PETTPLCVGVIIGITASIKICK

1

SDVKPETTPLCVGVIIGITASIKICK

26

92.3%

NZ_CP067069.1and 33 others:

NZ_CP085413.1 Lanthipeptides: LIS80_RS25380_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25375_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25370_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25365_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25360_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25355_lanthipeptideNZ_JH792105.1 Lanthipeptides: II5_RS26955_lanthipeptideNZ_LDKE01000067.1 Lanthipeptides: ABB04_RS19955_lanthipeptideNZ_LDKE01000067.1 Lanthipeptides: ABB04_RS19940_lanthipeptideNZ_LDKE01000067.1 Lanthipeptides: ABB04_RS19935_lanthipeptideNZ_MOOT01000061.1 Lanthipeptides: BK765_RS23995_lanthipeptideNZ_MOOT01000061.1 Lanthipeptides: BK765_RS23990_lanthipeptideNZ_NVEA01000001.1 Lanthipeptides: COL15_RS00005_lanthipeptideNZ_PIYQ01000022.1 Lanthipeptides: CW365_RS08185_lanthipeptideNZ_PIYQ01000022.1 Lanthipeptides: CW365_RS08180_lanthipeptideNZ_PIYQ01000022.1 Lanthipeptides: CW365_RS08175_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20670_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20665_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20660_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20655_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20650_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20645_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19555_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19550_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19545_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19540_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19535_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19530_lanthipeptideNZ_WBPE01000025.1 Lanthipeptides: F8161_RS14445_lanthipeptideNZ_WBPE01000025.1 Lanthipeptides: F8161_RS14440_lanthipeptideNZ_WBPK01000016.1 Lanthipeptides: F8167_RS15680_lanthipeptideNZ_WBPK01000016.1 Lanthipeptides: F8167_RS15675_lanthipeptideNZ_WBPK01000016.1 Lanthipeptides: F8167_RS15670_lanthipeptide

Lanthipeptides: JCR32_RS24625_lanthipeptide

1

SDVQPETTPLCVGVIIGITASIKICK

26

SDVQPETTPLCVGVIIG+T SIKICK

1

SDVQPETTPLCVGVIIGLTTSIKICK

26

with 110 more sequence matches

Only the first 10 are displayed here.

88.5%

NZ_CP016020.1:

Lanthipeptides: A9C19_RS17970_lanthipeptide

1

SDVQPETTPLCVGVIIGITASIKIC

25

SDVQPETTPLCVGVIIG+T SIKIC

1

SDVQPETTPLCVGVIIGLTTSIKIC

25

73.1%

NZ_JH792105.1:

Lanthipeptides: II5_RS26950_lanthipeptide

1

SDVQPETTPLCVGVIIGITAS

21

SDVQPETTPLCVGVIIG+T S

1

SDVQPETTPLCVGVIIGLTTS

21

71.4%

NZ_CP092966.1:

Lanthipeptides: KM149_RS02085_lanthipeptide

1

SDVQPETTPLCVGVIIGITASIKIC

25

SDV+PETTPLCVGVI G+T SIK C

1

SDVKPETTPLCVGVIAGLTYSIKKC

25

69.2%

NZ_JACSMH010000008.1and 2 others:

NZ_JACSMX010000006.1 Lanthipeptides: KNQ03_RS15615_lanthipeptideNZ_JACSMZ010000005.1 Lanthipeptides: KNQ06_RS16025_lanthipeptide

Lanthipeptides: KNP89_RS14470_lanthipeptide

2

DVQPETTPLCVGVIIGITASIKICK

26

DVQPETTP CVGVI+G+T S+ CK

2

DVQPETTPFCVGVIVGVTISLSKCK

26

65.4%

NZ_JAOVZP010000016.1and 3 others:

NZ_NHVL01000036.1 Lanthipeptides: CEU70_RS27720_lanthipeptideNZ_NTYE01000081.1 Lanthipeptides: CN490_RS28165_lanthipeptideNZ_NVMN01000027.1 Lanthipeptides: CON30_RS23205_lanthipeptide

Lanthipeptides: OF830_RS26725_lanthipeptide

1

SDVQPETTPLCV-GVIIGITASIKIC

25

SDVQPETTP CV + GI SIK C

1

SDVQPETTPACVTALTTGIIFSIKWC

26

63.0%

NZ_JAHHYI010000012.1:

Lanthipeptides: KM925_RS25405_lanthipeptide

1

SDVQPETTPLCVGVIIGITASIKICK

26

DVQPETTP CVG GIT I I K

1

GDVQPETTPACVGA--GITVGIAISK

24

60.7%

NZ_CP062165.1and 5 others:

NZ_CP062165.1 Lanthipeptides: JNUCC31_RS17170_lanthipeptideNZ_QICW01000005.1 Lanthipeptides: DEU73_RS14620_lanthipeptideNZ_QICW01000005.1 Lanthipeptides: DEU73_RS14620_lanthipeptideNZ_QLLI01000007.1 Lanthipeptides: DET54_RS17335_lanthipeptideNZ_QLLI01000007.1 Lanthipeptides: DET54_RS17335_lanthipeptide

Lanthipeptides: JNUCC31_RS17170_lanthipeptide

1

SDVQPETTPLC---VGVIIGITASIKIC

25

+DVQPETTPLC VGV IG+ S+ C

1

ADVQPETTPLCGFLVGVGIGVILSVTSC

28

60.7%

NZ_JAOVZP010000016.1and 1 other:

NZ_JAOVZP010000016.1 Lanthipeptides: OF830_RS26675_lanthipeptide

Lanthipeptides: OF830_RS26680_lanthipeptide

1

SDVQPETTPLC--VGVIIGITASIKICK

26

DV PETTPLC +GV GI S ICK

1

GDVSPETTPLCITIGVGGGIAISKLICK

28

60.7%

NZ_LN831278.1:

Lanthipeptides: BN1360_RS08840_lanthipeptide

1

SDVQPETTPLC---VGVIIGITASIKIC

25

+DVQPETTPLC VGV IG+ S+ C

1

ADVQPETTPLCGFFVGVGIGVILSVAKC

28

59.3%

NZ_FTML01000041.1:

Lanthipeptides: BW937_RS20030_lanthipeptide

1

SDVQPETTPLCVGVIIGITASIKICK

26

DVQPE+TP CVG GIT I I K

1

GDVQPESTPACVGA--GITVGIAISK

24

MIBiG 4.0 matches for BK765_RS23985

18.9%

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

7

TTPLCVGVIIGITA

20

T P G+I+GI A

1

TDPAVTGLILGIVA

14

BK765_RS23990

Leader:

MTKGYKFTKEELVEAWKDPQVREKLKDLPKHPSGKALNELSEEELAEIQGA

BK765_RS23995

Leader:

MTKGYKFTKEELVEAWKDPQVREKLKDLPKHPSGKALNELSEEELAEIQGA

Shared core:

SDVQPETTPLCVGVIIGLTTSIKICK

Core with dehydrations:

DhaDVQPEDhbDhbPLCysVGVIIGLDhbDhbDhaIKICysK

antiSMASH-DB 4.0 matches for BK765_RS23990

100.0%

NZ_CP067069.1and 33 others:

NZ_CP085413.1 Lanthipeptides: LIS80_RS25380_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25375_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25370_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25365_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25360_lanthipeptideNZ_CP085413.1 Lanthipeptides: LIS80_RS25355_lanthipeptideNZ_JH792105.1 Lanthipeptides: II5_RS26955_lanthipeptideNZ_LDKE01000067.1 Lanthipeptides: ABB04_RS19955_lanthipeptideNZ_LDKE01000067.1 Lanthipeptides: ABB04_RS19940_lanthipeptideNZ_LDKE01000067.1 Lanthipeptides: ABB04_RS19935_lanthipeptideNZ_MOOT01000061.1 Lanthipeptides: BK765_RS23995_lanthipeptideNZ_MOOT01000061.1 Lanthipeptides: BK765_RS23990_lanthipeptideNZ_NVEA01000001.1 Lanthipeptides: COL15_RS00005_lanthipeptideNZ_PIYQ01000022.1 Lanthipeptides: CW365_RS08185_lanthipeptideNZ_PIYQ01000022.1 Lanthipeptides: CW365_RS08180_lanthipeptideNZ_PIYQ01000022.1 Lanthipeptides: CW365_RS08175_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20670_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20665_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20660_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20655_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20650_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20645_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19555_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19550_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19545_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19540_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19535_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19530_lanthipeptideNZ_WBPE01000025.1 Lanthipeptides: F8161_RS14445_lanthipeptideNZ_WBPE01000025.1 Lanthipeptides: F8161_RS14440_lanthipeptideNZ_WBPK01000016.1 Lanthipeptides: F8167_RS15680_lanthipeptideNZ_WBPK01000016.1 Lanthipeptides: F8167_RS15675_lanthipeptideNZ_WBPK01000016.1 Lanthipeptides: F8167_RS15670_lanthipeptide

Lanthipeptides: JCR32_RS24625_lanthipeptide

1

SDVQPETTPLCVGVIIGLTTSIKICK

26

SDVQPETTPLCVGVIIGLTTSIKICK

1

SDVQPETTPLCVGVIIGLTTSIKICK

26

96.2%

NZ_CP016020.1:

Lanthipeptides: A9C19_RS17970_lanthipeptide

1

SDVQPETTPLCVGVIIGLTTSIKIC

25

SDVQPETTPLCVGVIIGLTTSIKIC

1

SDVQPETTPLCVGVIIGLTTSIKIC

25

92.3%

NZ_CP067069.1and 13 others:

NZ_CP085413.1 Lanthipeptides: LIS80_RS25350_lanthipeptideNZ_JH792105.1 Lanthipeptides: II5_RS26960_lanthipeptideNZ_JH792240.1 Lanthipeptides: IIK_RS26240_lanthipeptideNZ_MOOT01000061.1 Lanthipeptides: BK765_RS23985_lanthipeptideNZ_NTUF01000024.1 Lanthipeptides: CN379_RS26680_lanthipeptideNZ_NVCZ01000001.1 Lanthipeptides: COK75_RS01180_lanthipeptideNZ_NVDK01000008.1 Lanthipeptides: COK95_RS05665_lanthipeptideNZ_NVEA01000001.1 Lanthipeptides: COL15_RS00010_lanthipeptideNZ_PIYQ01000022.1 Lanthipeptides: CW365_RS08190_lanthipeptideNZ_WBOZ01000055.1 Lanthipeptides: F8156_RS20675_lanthipeptideNZ_WBPC01000027.1 Lanthipeptides: F8159_RS19560_lanthipeptideNZ_WBPE01000025.1 Lanthipeptides: F8161_RS14435_lanthipeptideNZ_WBPK01000016.1 Lanthipeptides: F8167_RS15685_lanthipeptide

Lanthipeptides: JCR32_RS24620_lanthipeptide

1

SDVQPETTPLCVGVIIGLTTSIKICK

26

SDVQPETTPLCVGVIIG+T SIKICK

1

SDVQPETTPLCVGVIIGITASIKICK

26

with 101 more sequence matches

Only the first 10 are displayed here.

88.5%

NZ_LDKE01000067.1:

Lanthipeptides: ABB04_RS19960_lanthipeptide

1

SDVQPETTPLCVGVIIGLTTSIKICK

26

SDV+PETTPLCVGVIIG+T SIKICK

1

SDVKPETTPLCVGVIIGITASIKICK

26

80.8%

NZ_JH792105.1:

Lanthipeptides: II5_RS26950_lanthipeptide

1

SDVQPETTPLCVGVIIGLTTS

21

SDVQPETTPLCVGVIIGLTTS

1

SDVQPETTPLCVGVIIGLTTS

21

75.0%

NZ_CP092966.1:

Lanthipeptides: KM149_RS02085_lanthipeptide

1

SDVQPETTPLCVGVIIGLTTSIKIC

25

SDV+PETTPLCVGVI GLT SIK C

1

SDVKPETTPLCVGVIAGLTYSIKKC

25

69.2%

NZ_JACSMH010000008.1and 2 others:

NZ_JACSMX010000006.1 Lanthipeptides: KNQ03_RS15615_lanthipeptideNZ_JACSMZ010000005.1 Lanthipeptides: KNQ06_RS16025_lanthipeptide

Lanthipeptides: KNP89_RS14470_lanthipeptide

1

SDVQPETTPLCVGVIIGLTTSIKICK

26

DVQPETTP CVGVI+G+T S+ CK

1

GDVQPETTPFCVGVIVGVTISLSKCK

26

61.5%

NZ_JACSPU010000002.1:

Lanthipeptides: H9630_RS06775_lanthipeptide

1

SDVQPETTPLCVGVII-GLTTSIKIC

25

SDV PETTP CV GL SIK C

1

SDVAPETTPACVAAFATGLLFSIKFC

26

61.5%

NZ_JAOVZP010000016.1and 3 others:

NZ_NHVL01000036.1 Lanthipeptides: CEU70_RS27720_lanthipeptideNZ_NTYE01000081.1 Lanthipeptides: CN490_RS28165_lanthipeptideNZ_NVMN01000027.1 Lanthipeptides: CON30_RS23205_lanthipeptide

Lanthipeptides: OF830_RS26725_lanthipeptide

1

SDVQPETTPLCV-GVIIGLTTSIKIC

25

SDVQPETTP CV + G+ SIK C

1

SDVQPETTPACVTALTTGIIFSIKWC

26

60.7%

NZ_CP062165.1and 5 others:

NZ_CP062165.1 Lanthipeptides: JNUCC31_RS17170_lanthipeptideNZ_QICW01000005.1 Lanthipeptides: DEU73_RS14620_lanthipeptideNZ_QICW01000005.1 Lanthipeptides: DEU73_RS14620_lanthipeptideNZ_QLLI01000007.1 Lanthipeptides: DET54_RS17335_lanthipeptideNZ_QLLI01000007.1 Lanthipeptides: DET54_RS17335_lanthipeptide

Lanthipeptides: JNUCC31_RS17170_lanthipeptide

1

SDVQPETTPLC---VGVIIGLTTSIKIC

25

+DVQPETTPLC VGV IG+ S+ C

1

ADVQPETTPLCGFLVGVGIGVILSVTSC

28

60.7%

NZ_LN831278.1:

Lanthipeptides: BN1360_RS08840_lanthipeptide

1

SDVQPETTPLC---VGVIIGLTTSIKIC

25

+DVQPETTPLC VGV IG+ S+ C

1

ADVQPETTPLCGFFVGVGIGVILSVAKC

28

59.3%

NZ_JAHHYI010000012.1:

Lanthipeptides: KM925_RS25405_lanthipeptide

1

SDVQPETTPLCVGVIIGLTTSIKICK

26

DVQPETTP CVG G+T I I K

1

GDVQPETTPACVGA--GITVGIAISK

24

58.6%

NZ_JAOVZP010000016.1and 3 others:

NZ_NHVL01000036.1 Lanthipeptides: CEU70_RS27725_lanthipeptideNZ_NTYE01000081.1 Lanthipeptides: CN490_RS28170_lanthipeptideNZ_NVMN01000027.1 Lanthipeptides: CON30_RS23200_lanthipeptide

Lanthipeptides: OF830_RS26730_lanthipeptide

1

SDVQPETTPLC-VGVIIGLTT--SIKICK

26

SDVQPETTP C V IG T S++ CK

1

SDVQPETTPACAVAFTIGATVIFSVRNCK

29

MIBiG 4.0 matches for BK765_RS23990

19.2%

BGC0000580.3 (EGD17355.1):

Lasso peptide: xanthomonin II or xanthomonin I

9

PLCVGVIIGLTT

20

PL + G+TT

3

PLAGEEMGGITT

14

13.5%

BGC0001229.4 (SBI_06989):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

7

TTPLCVGVIIGL

18

T P G+I+G+

1

TDPAVTGLILGI

12

11.8%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

20

TSIKIC

25

TSI +C

2

TSISLC

7

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

BK765_RS23965: PT_FPPS_like

Domain 1 (59 - 305): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 20.05 out of 23.43

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 17.07 out of 18.16

Confidence: strong

Lanthipeptides

Terpene

TFBS Finder

Core peptide predictions

BK765_RS23985 - Class II

Cleavage pHMM score: 4.20

RODEO score: 0

Monoisotopic mass: 2594.4 Da

Molecular weight: 2596.1 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2614.1 Da N = 1; 2632.1 Da N = 2; 2650.2 Da N = 3

BK765_RS23990 - Class II

Cleavage pHMM score: 4.20

RODEO score: 0

Monoisotopic mass: 2606.5 Da

Molecular weight: 2608.1 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2626.1 Da N = 1; 2644.1 Da N = 2; 2662.2 Da N = 3; 2680.2 Da N = 4

BK765_RS23995 - Class II

Cleavage pHMM score: 4.20

RODEO score: 0

Monoisotopic mass: 2606.5 Da

Molecular weight: 2608.1 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2626.1 Da N = 1; 2644.1 Da N = 2; 2662.2 Da N = 3; 2680.2 Da N = 4

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
BldD	Development and antibiotic global regulator

NZ_MOOT01000062.1 - Region 1 - azole-containing-RiPP

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Location: 298,825 - 322,331 nt. (total: 23,507 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

BK765_RS25620: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001174.4	0.44	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0002013.4	0.44	ribosomal	curacozole	Streptomyces curacoi
BGC0000607.5	0.43	ribosomal	micrococcin P1	Macrococcus caseolyticus
BGC0001535.3	0.43	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0001146.5	0.41	ribosomal	cyclothiazomycin C	Streptomyces sp. NRRL WC-3908
BGC0001173.5	0.41	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0000606.5	0.39	ribosomal	lactazole	Streptomyces lactacystinaeus
BGC0001145.5	0.38	ribosomal	cyclothiazomycin b1	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0001640.3	0.38	ribosomal	kocurin	Kocuria rosea
BGC0000605.5	0.38	ribosomal	GE37468	Streptomyces sp. ATCC 55365

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001174.4	0.44	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0002013.4	0.44	ribosomal	curacozole	Streptomyces curacoi
BGC0000607.5	0.43	ribosomal	micrococcin P1	Macrococcus caseolyticus
BGC0001535.3	0.43	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0001146.5	0.41	ribosomal	cyclothiazomycin C	Streptomyces sp. NRRL WC-3908
BGC0001173.5	0.41	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0000606.5	0.39	ribosomal	lactazole	Streptomyces lactacystinaeus
BGC0001145.5	0.38	ribosomal	cyclothiazomycin b1	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0001640.3	0.38	ribosomal	kocurin	Kocuria rosea
BGC0000605.5	0.38	ribosomal	GE37468	Streptomyces sp. ATCC 55365

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.57 out of 18.57

Confidence: strong

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 22.11 out of 27.55

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.60 out of 26.06

Confidence: medium

(Thi)azol(in)e containing peptide predictions

No core peptides found.

RREFinder

TFBS Finder

Azole RiPPs

RRE predictions

: RRE type: Heterocycloanthracin_C_RRE; Location: 0 - 100; Score: 184.8; RRE type: Thiopeptide_F_RRE; Location: 10 - 89; Score: 41.3

TFBS definitions

Regulator	Description
GnfM	Regulator of nitrogen-fixation genes
MogR	Repressor of flagellar motility genes
ToxT	Regulator of MSHA biosynthesis

Core peptide predictions

No core peptides found.

NZ_MOOT01000065.1 - Region 1 - t3nrps-iterative

Download region SVG

Location: 110,857 - 158,399 nt. (total: 47,543 nt) Show pHMM detection rules used

t3nrps-iterative: (AMP-binding and PP-binding and (DUF98 or Orn_Arg_deC_N or Orn_DAP_Arg_deC) and not ATP-grasp)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acetyltransferase aminotransferase glycosyltransferase transferase

BK765_RS29295: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

BK765_RS29305: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

BK765_RS29415: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

BK765_RS29420: Chloramphenicol acetyltransferase

smcogs: SMCOG1156: Chloramphenicol acetyltransferase

Hydrolases: amidase hydrolase

BK765_RS29220: amidase

smcogs: SMCOG1105: amidase

BK765_RS29315: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

Lyases: dehydratase

BK765_RS29300: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

BK765_RS29245: cobalamin synthesis protein/P47K family protein

smcogs: SMCOG1178: cobalamin synthesis protein/P47K family protein

BK765_RS29320: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002450.2	0.53	other (other)	sarasinoside	uncultured bacterium
BGC0000820.5	0.50	other (other)	physostigmine	Streptomyces griseofuscus
BGC0000410.5	0.49	NRPS	pseudomonine	Pseudomonas fluorescens
BGC0000699.5	0.47	saccharide	hygromycin A	Streptomyces hygroscopicus subsp. hygroscopicus
BGC0002674.2	0.46	ribosomal	enterocin F4-9	Enterococcus faecalis
BGC0001488.3	0.46	other (other)	4-formylaminooxyvinylglycine	Pseudomonas fluorescens WH6
BGC0000294.5	0.45	NRPS	acinetobactin	Acinetobacter baumannii
BGC0001747.3	0.45	other (other)	pseudaminic acid	Tannerella forsythia
BGC0000773.3	0.45	saccharide	lipopolysaccharide	Escherichia coli
BGC0002042.3	0.45	NRPS	anthelvencin A, anthelvencin B, anthelvencin C	Streptomyces venezuelae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002450.2	0.53	other (other)	sarasinoside	uncultured bacterium
BGC0000820.5	0.50	other (other)	physostigmine	Streptomyces griseofuscus
BGC0000410.5	0.49	NRPS	pseudomonine	Pseudomonas fluorescens
BGC0000699.5	0.47	saccharide	hygromycin A	Streptomyces hygroscopicus subsp. hygroscopicus
BGC0002674.2	0.46	ribosomal	enterocin F4-9	Enterococcus faecalis
BGC0001488.3	0.46	other (other)	4-formylaminooxyvinylglycine	Pseudomonas fluorescens WH6
BGC0000294.5	0.45	NRPS	acinetobactin	Acinetobacter baumannii
BGC0001747.3	0.45	other (other)	pseudaminic acid	Tannerella forsythia
BGC0000773.3	0.45	saccharide	lipopolysaccharide	Escherichia coli
BGC0002042.3	0.45	NRPS	anthelvencin A, anthelvencin B, anthelvencin C	Streptomyces venezuelae

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 24.11 out of 30.25

Confidence: medium

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 22.18 out of 27.55

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.21 out of 26.06

Confidence: medium

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.35 out of 27.55

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 26.74 out of 27.78

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.81 out of 20.97

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.62 out of 26.06

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 25.75 out of 28.19

Confidence: strong

Additional weak hits (3):

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.37 out of 25.88

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 18.76 out of 30.11

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 18.69 out of 30.12

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

BK765_RS29320: X

AMP-binding (7..395): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: EQLAHAGFAFGAIALTAGGNNVYGMTETGAISIL
Nearest Stachelhaus code(s):: Val DALVLAVAIK (35% 8Å match)
Stachelhaus code match:: 60% (weak)

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
ColR	Phenol-responsive regulator
GnfM	Regulator of nitrogen-fixation genes
MogR	Repressor of flagellar motility genes
ZuR variant 1	Zinc-responsive repressor

NZ_MOOT01000065.1 - Region 2 - NI-siderophore