LOCUS       NZ_MOOT01000061        18579 bp    DNA     linear   CON 20-MAY-2024
DEFINITION  Bacillus thuringiensis serovar dakota strain BGSC 4R1 contig000061,
            whole genome shotgun sequence.
ACCESSION   NZ_MOOT01000061
VERSION     NZ_MOOT01000061.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis serovar dakota
  ORGANISM  Bacillus thuringiensis serovar dakota
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 81613)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Comparative genomics of Bacillus thuringiensis reveals a path to
            pathogens against multiple invertebrate hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 81613)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, 1st Shizishan,
            Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.5.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002148095.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 01:21:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,175
            CDSs (total)                      :: 6,055
            Genes (coding)                    :: 5,836
            CDSs (with protein)               :: 5,836
            Genes (RNA)                       :: 120
            rRNAs                             :: 4, 7, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 7, 6 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 88 of 219
            Pseudo Genes (incomplete)         :: 138 of 219
            Pseudo Genes (internal stop)      :: 66 of 219
            Pseudo Genes (multiple problems)  :: 56 of 219
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:21:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 63034
            Orig. end                         :: 81613
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOOT01000061.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..18579
                     /candidate_cluster_numbers="1"
                     /candidate_cluster_numbers="2"
                     /contig_edge="True"
                     /product="terpene-precursor"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     cand_cluster    1..18579
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="neighbouring"
                     /product="terpene-precursor"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    1..18579
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[10000:10930](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10930
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    137..150
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.05"
                     /tool="antismash"
     gene            complement(397..1425)
                     /gene="cggR"
                     /locus_tag="BK765_RS23920"
                     /old_locus_tag="BK765_22720"
     CDS             complement(397..1425)
                     /codon_start=1
                     /gene="cggR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258191.1"
                     /locus_tag="BK765_RS23920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22720"
                     /product="gapA transcriptional regulator CggR"
                     /protein_id="WP_001258185.1"
                     /transl_table=11
                     /translation="MRSWIQNTKKLLPDLLPVMQTRMQILQYIRLMQPIGRRNLSASLG
                     MTERVLRSEVQVLKEQNLVHVASSGMTLTEEGTALVLALEDFMKEISGLKVLEKQLKET
                     LDLDEVFVVPGDSDESPWVKLEMGRACVTCIKDRLTANNIVAVAGGTTLAAAADMMQLD
                     CKDLHMLFVPARGGIGEGVELEANTICAKMAQNTMSNYRLLYVPDHVSSEAYASIVTEP
                     SVKEVLELIRSSNIVIHGIGDALTMARRRSTSEADWLKIQASEAVGEAFGYYFNEEGNV
                     VHKVRTVGMQLEDLQNVSHVVAVAGGSSKAKAIQAVIKQGHTSILITDEGAAKQLTKGF
                     TL"
     gene            complement(1562..1807)
                     /locus_tag="BK765_RS23925"
                     /old_locus_tag="BK765_22725"
     CDS             complement(1562..1807)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657034.1"
                     /locus_tag="BK765_RS23925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22725"
                     /product="glutaredoxin family protein"
                     /protein_id="WP_000869737.1"
                     /transl_table=11
                     /translation="MKVVLYTKKNCGLCVQAKQVLQEVQCEYSFQIEEIDIYEDNDLLE
                     KYHLMIPVVEIDGKQVEYGNIHKGVIINYIKNAVKS"
     gene            complement(1817..3124)
                     /gene="rpoN"
                     /locus_tag="BK765_RS23930"
                     /old_locus_tag="BK765_22730"
     CDS             complement(1817..3124)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpoN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647943.1"
                     /locus_tag="BK765_RS23930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22730"
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="WP_000647951.1"
                     /transl_table=11
                     /translation="MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENP
                     LIELGGFEREKKKSSNNSKSTSKQVENQMEIYSVDSTTIQQHLLNQIQYYKIDEEERKV
                     ASFIIMNMDGNGYLQETNEELAELLSAHIHVVDRSVELVQSLEPAGVGARNIQECLTLQ
                     LKRLQRRDGLAEDIVEEHFAYFVKKDWRKLVHVLKCSNEELQSAVNCITSLQPKPGLAY
                     SSDKPLYIVPDMAVKKEGDRLVLQMNERNMPRIEIHSEYSALLNNSESEVASYVSEKYQ
                     HVQWIMRSLKQRKQTLLQVMTIIMEKQRDFFWEGPAYLKPLALKEVAEELSVHESTISR
                     ATRNKYVQTPHGLFEMKSFFSNAVSTTEDEAVSTKRVKQFIQTLVEAENKKKPLSDQKI
                     SKLLEEEHEIVISRRTVAKYREQMHIPASSLRKTIG"
     gene            complement(3427..3624)
                     /locus_tag="BK765_RS23935"
                     /old_locus_tag="BK765_22735"
     CDS             complement(3427..3624)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505575.1"
                     /locus_tag="BK765_RS23935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22735"
                     /product="hypothetical protein"
                     /protein_id="WP_000505575.1"
                     /transl_table=11
                     /translation="MGFFSKRKQKMMDSNNERSVKKIVELSVEEMRGIQGASSESNDYL
                     YDSYVIRNQSSLALNKRFLS"
     gene            complement(3644..4864)
                     /locus_tag="BK765_RS23940"
                     /old_locus_tag="BK765_22740"
     CDS             complement(3644..4864)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243605.1"
                     /locus_tag="BK765_RS23940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22740"
                     /product="ABC transporter permease"
                     /protein_id="WP_086398397.1"
                     /transl_table=11
                     /translation="MRNFWIVLGQTYWDRVRTKIFIGMTLLLIGGGIFLFSFPTLVQKF
                     SGEKEKAKVVFISEAPNVTLNQSNLSKALPEWDWSLGDKTKLNQYKQDLLDKKVEALFI
                     LKEGAGAEPVLDYFMKVDNPKLIQSAQVFVQNQYFQSAVVKQQLSEEAVKSLTMNVQAK
                     KQNLNNEETSSFLLVYLLVAIMYLAITSYGNAIATAVASEKASRVMEVMVTKVSPVHMV
                     FGKILGVGLASLTQLFIFFLSMALYIKSGIFKVSDSFFGVNIDVNMITGEHAAYFLLYF
                     VLGYFVYAALFAVFGSMVSRPEELSGTTMPITLILMGSLVVELLVVLDNPEGLVSRVTS
                     YVPFTAPISMVVRIINGSVGSLEIITSMVVLLASISLIAMFAAKVYPKGVLRSGKSLKF
                     IQLLKNN"
     cand_cluster    4195..18579
                     /candidate_cluster_number="2"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    4195..18579
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[14194:17353](-)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      complement(14195..17353)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="2"
     gene            complement(4868..5752)
                     /locus_tag="BK765_RS23945"
                     /old_locus_tag="BK765_22745"
     CDS             complement(4868..5752)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863488.1"
                     /locus_tag="BK765_RS23945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22745"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_000863495.1"
                     /transl_table=11
                     /translation="MAFEIQGLNKTFGEYKAVNDLNIRVEEGGILGMLGRNGAGKTTTI
                     RMILDLIKPDSGQILWNNRPFSKKDLSIGYLPEERGLYPKMNVVEQLVYFGKLEGMPAN
                     LAKRHALQWLEKLEMTPHLKKKTEELSKGNQQKIQLISAILHNPEFIILDEPFSGLDPV
                     NAEMLKNVVLELIAQRKTIIFCSHQMDSVETFCEEICIMKNGQLVLSGALSDIKKSYQY
                     KYMTLQTEGDIEPILKNKNFNFEKVNRTFMIKVNDGEDAFSLLEDIKTKQYIRDVSIKE
                     PSLHQIFVEKAGA"
     gene            complement(5847..7319)
                     /locus_tag="BK765_RS23950"
                     /old_locus_tag="BK765_22750"
     CDS             complement(5847..7319)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656184.1"
                     /locus_tag="BK765_RS23950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22750"
                     /product="S8 family serine peptidase"
                     /protein_id="WP_000656184.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 2.8e-42, bitscore:
                     137.2, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDWPKYRNTIAKIGAGITVGLLLNLPIVGISTISAEEMKHNQIK
                     ENSYVFTLRQGENTDEMIKEIRDKYPDLKLEVIKEIKMLNIEGDNLERVQEAKQHLLKN
                     YKGIIEKAGREDVVELKPPAIVKKPSNITKYAPSQLEQEEVDYSKWKWDVDKVTENGES
                     YKIEQGNHSVKIGVIDSGIDVNHPALKGNIVSQGKILVPNVESIEDNIGHGTMIAGLIA
                     ADGKIKGVAPKIGIVPYKVFQGSSADSSWIIKAIVEAANDGMDVINLSLGTYKSIKDPE
                     EGAVYLAYKRAIEYANSKGSLLVASSGTEGFDISNPFQLAKQRGYENDLQLHMPGGLPG
                     VVTVAGTNKDDRLAYYSNYGTNVDIAAPSGDYGSLWESEKVGDETAMVLTTYPTNLPQS
                     QLSEWLGFDRGYELMIGTSLAVPKVSATAALVIAEYKEKFRLKPSNGFVKTRLYQGAKP
                     ALDDGKKYFGKGIVNAKGALDFRNTNFKKWER"
     gene            complement(7303..9459)
                     /locus_tag="BK765_RS23955"
                     /old_locus_tag="BK765_22755"
     CDS             complement(7303..9459)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151549.1"
                     /locus_tag="BK765_RS23955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22755"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_001151549.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.4e-42, bitscore:
                     136.9, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKVPFIEQMEHSECGLASLAMVFSFYEKHVSLAELREEFGVPKG
                     GTSLYQLMHIARKNGFQVKGYKAEYEALSEVKLPAVIHWEHKHYVVLEKVTKKGAIVID
                     PAYGRNKISKKEMEEKYSGFVLTLEPDESFQKQKGVSHLQFFLSFVLGKKKLIALIVLA
                     SLIIQAFALVIPFVTSWMTDHVIIPRDPSYMNMIGYSILVLLISYQVFASLRGFLIAKL
                     QTAIDMSLMQSFIEKLLSLSYNFFENRSMGELLFRANSNVYIRQILSTKVISFLIDGIL
                     LFTYLFFMFRYSTLLGFVVLAVGSVIFALLVMSTTVSKKLADKEVSAQAQVQRILSESI
                     NGISDVKVMGLEKQVYKEWQERFGSQLIHAEKRSIWTALMNTLAGSIQFILPLFLLWFS
                     GQQVLAGNMTLGSVLGFNALAISFITPIISIGSGYGELIYISSYIQRIYDVMHARSERE
                     VNQFSGIKKLNGDIEFDNVSYRHNYFSEDAIQNVSFKIKAGEKVAIVGASGSGKSTIVK
                     MLLGLYTPTNGKIRFDGIESEKLNLKFLRKSIGVVFQEARLFNKTVGENIIAQRKGVPP
                     NGVIEASKQANILEDIMMLPLNFQTMVSEFGVNFSGGQRQRLILARALVTNPSILILDE
                     ATSALDTLSERVIDENLTDMLCTRIVIAHRLSTVKNADKIIVMDKGAIVEIGSHTELLE
                     KQGYYYQLSKAQEVKGGIKSERLA"
     gene            complement(9479..9733)
                     /locus_tag="BK765_RS23960"
                     /old_locus_tag="BK765_22760"
     CDS             complement(9479..9733)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757187.1"
                     /locus_tag="BK765_RS23960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22760"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000757187.1"
                     /transl_table=11
                     /translation="MKKVLSGREKEVAVLIARGLKDVEIARELFISRRRVGELVASIKR
                     KLEIHSRVQIGIAAYFLGLITFEFNFNHGNHEGVAMINS"
     gene            10001..10930
                     /locus_tag="BK765_RS23965"
                     /old_locus_tag="BK765_22765"
     CDS             10001..10930
                     /EC_number="2.5.1.-"
                     /GO_function="GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640555.1"
                     /locus_tag="BK765_RS23965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22765"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_000640554.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 2.8e-20, bitscore:
                     64.7, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIYSLQNELILRAKKLVYGHFFESTLRQCSSEYIEYKFNDSTRFA
                     NITLVHYEIFQDNQNEEEKQLLLTIIELLMLALDIMDDIQDEDFLEGPWTKHPIATNLN
                     IIMGFLLICLQEVDKASLSIQMKNWLKNEIHSSILNSINGQQLDLKNEIHSEKDYIDMV
                     THKSGYLIKLACLLGTGNINPIQRSLIENYALCIGVINQIKNDMRDIMQWDKKNDFLNL
                     KKTLPILYYLNSKNDNFILIKKFFNQEIQYDELNNQTLDSLKHILLYGGSMEYCSVMQN
                     LYVYKSKGYIEQLSLSQANKDRLIDTLI"
     gene            complement(10932..11099)
                     /locus_tag="BK765_RS32585"
     CDS             complement(10932..11099)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617614.1"
                     /locus_tag="BK765_RS32585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000617614.1"
                     /transl_table=11
                     /translation="MIRELVERILEDIQFKNVCSQGTIAIDGISKQELEIILGTLNKSS
                     LLSKAAETWK"
     gene            11216..13531
                     /locus_tag="BK765_RS23970"
                     /old_locus_tag="BK765_22770"
     CDS             11216..13531
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017762423.1"
                     /locus_tag="BK765_RS23970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22770"
                     /product="sensor histidine kinase"
                     /protein_id="WP_000595466.1"
                     /transl_table=11
                     /translation="MIKQRPIYFVISLIIFYLIIQSYILYKFYQPYSGIIASKEKHNTY
                     TISQIDTSGWAAKTALKAGDEIYKVDGANPYLGVFKRYQNQFLNATEIQTIQNDRISTY
                     STTATSDNEELIITLYLPMIFSLTILSLCFIVYRNKAIRREERYFMLFLLATSISLSAS
                     SISSKSDFGGLLIISISFQSVSLFLTLFLYQLFKRKEIIIIHKNVIRFHIFLYMTVITT
                     GLLSVTFLPNLQQVSAYTDLAYLCFSILLSVYFLLKGYIKYRNTLHRPFFKFMLVIQCI
                     SFGPFIMLYALPRILFNAAIIRADIAALFLFCIPIGYFYLILTKQIFDIDFMFDRVRYY
                     SLLSLLPTIIITLIISWGINPDERFFIRLMQNFIIIFPLNILFLVIKERLDFTFRNQLF
                     RSKTNLHVSIDQFAQQLSSIMKVKDLEKKFMNEIISLLKPCFIQFIEFDTKQNTYNCYK
                     SYGETEKFNLSKKKQWGMESGYINDLLEDDGCIGIYLYSTQNTKHYIWVGNKTNNIKFN
                     INEKTWFIAIVKYVRLVYENLNSVNQIIQSLEKESIANKTHSATLSRLLFQISETERRR
                     LAADLHDAALQDQIVWYRKLETVLKEHTVPGELQGQLEKIKFGMFDVIQQIRDTCNELK
                     PALLSEIGLIGALKELLAQMQLRTNFRIQFEYDSIQENHYEFDKILSIYRIIQELLNNA
                     DKHSNATLINIFLWEENNCIYLDYRDNGIGFNTQAPYSSNKHMGLSGIKGRVQGIGGEI
                     QLISEIGNGLQVNITIPR"
     gene            13537..14223
                     /locus_tag="BK765_RS23975"
                     /old_locus_tag="BK765_22775"
     CDS             13537..14223
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621767.1"
                     /locus_tag="BK765_RS23975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22775"
                     /product="response regulator transcription factor"
                     /protein_id="WP_086398398.1"
                     /transl_table=11
                     /translation="MIHVLIVDDHQIVGEGTKNMIELEEDIQATYILNEQEAISQSSKT
                     PFDIYLLDMNMPNYSGLDVAQKLLHIQPDAKIILYTGFEYLSQFNLLIDSGISGIVSKS
                     ASQQELLMAIRAVLNGYTLIPISLLRQLRVEDMTVDNLDTKRASSHVTITQKELEILES
                     IAKGKSNKQITDELYMSSRAVEYNLTKLFKKFKVSSRSEALAEAVRKGIINVNVFSFIL
                     LHFLMS"
     gene            complement(14195..17353)
                     /locus_tag="BK765_RS23980"
                     /old_locus_tag="BK765_22780"
     CDS             complement(14195..17353)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905305.1"
                     /locus_tag="BK765_RS23980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22780"
                     /product="type 2 lanthipeptide synthetase LanM family
                     protein"
                     /protein_id="WP_086398399.1"
                     /sec_met_domain="DUF4135 (E-value: 5.2e-107, bitscore:
                     350.5, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 5.3e-78, bitscore:
                     254.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLIHSYINEKAFAKSLNLKERYKTLKSRRDLLLSEDLNLAQLNKW
                     IDKMFRSNKADFDFKLEMNEMTKEDFASLIEKIPESKEHLYIDELNEHVNLRVLEIGIE
                     LCESKPLSAEEDLGFIEFVRPFALYAAEMIDSSLKYRLNRIRYEHEEIKEMLVRSLVEE
                     LLNNAVRSLVLEFNIAKLREELVGETPEERFLSFVREKARNKENLIAFYEEYATLTRRL
                     ILRTEYFIQNVQMLLFRLNENWLQLQKEFQLQDVSLSEIKVGLGDTHQEGKTVVQLVFS
                     SGKEILYKPKSMEITKSYHAFLEWMNEVSGSIQLPSYKVLDFGEYGWEEKILYKPCSSK
                     EEVERYYLRFGKLIGLMHMLKGADLHFENIIAHGEYPYIIDSETIFHQYPKLNFPDSAE
                     VKLKYEQSDSVIGSGLLPQAMFQNIDGKGIDLSALNGKEQDLPFKVLALSQNSKDEMEF
                     TMKEAKSQSAKNLPKLGVEEIYVEDYLTFIIDGFQEMCKFFIEYKEEILSERGPLITFR
                     ENKIRIVARATQQYCNFLQESTHPDYMRDSILLEQLFERVWYYPYENKGLVIHEIQDLL
                     RADVPIFTTFTDSRDLYSSTGERMEDFFKESGYEQVYNRIYHFDDKELEKQRGWLKLAI
                     QGNRDIDIKVRRKEDYRSELLENHQETILQEAINIGESLVENVVLSDDKTTASWLQANV
                     IHDKWYVAPMKQNLYDGLGGVALFLLYLNKVTGNEKYLEVAHKALKSAMNPLSKAKGLL
                     STFLGKYSLLYPLAHFQSISPRKEYQDFLEEIKVELKERLKEEEEFDLLSGSAGVIQVA
                     LNLFEMTKDQDYLDLADMYSKHLVENVSILESGVAWKNKHTNSYLGGFSHGTSGIAWSL
                     WRSGIMNRNQEYIDLAKKAFAYDRSLFEKEESAWKDTRESEKKCLHQWCHGSTGIGLSR
                     VLVRSYEEDVEMDEEIRVAVDNVRKYGFKNNDTLCHGNMGDTELLLAVSQLYGNKELYE
                     QALQIGIQVINNYRETGSYQCDAPDDIQSFGMFVGIAGVGMQLLRLLQPNEIPSVMTLE
                     NVKG"
     misc_feature    complement(17402..17411)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 17.07"
                     /tool="antismash"
     gene            complement(17508..17741)
                     /locus_tag="BK765_RS23985"
                     /old_locus_tag="BK765_22785"
     CDS             complement(17508..17741)
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002103816.1"
                     /locus_tag="BK765_RS23985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22785"
                     /product="mersacidin/lichenicidin family type 2
                     lantibiotic"
                     /protein_id="WP_000032577.1"
                     /transl_table=11
                     /translation="MSKEYKFTKEELVEAWKDPQVREKLKDLPKHPSGKALNELSEEEL
                     AEIQGASDVQPETTPLCVGVIIGITASIKICK"
     CDS_motif       complement(17589..17741)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     MSKEYKFTKEELVEAWKDPQVREKLKDLPKHPSGKALNELSEEELAEIQGA"
                     /locus_tag="BK765_RS23985_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(17508..17588)
                     /prepeptide="core"
                     /locus_tag="BK765_RS23985_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="4.20"
                     /molecular_weight="2596.1"
                     /monoisotopic_mass="2594.4"
                     /core_sequence="SDVQPETTPLCVGVIIGITASIKICK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MSKEYKFTKEELVEAWKDPQVREKLKDLPKHPSGKALNELS
                     EEELAEIQGA"
                     /leader_location="[17588:17741](-)"
                     /alternative_weights="2614.1"
                     /alternative_weights="2632.1"
                     /alternative_weights="2650.2"
     gene            complement(17773..18006)
                     /locus_tag="BK765_RS23990"
                     /old_locus_tag="BK765_22790"
     CDS             complement(17773..18006)
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002103816.1"
                     /locus_tag="BK765_RS23990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22790"
                     /product="mersacidin/lichenicidin family type 2
                     lantibiotic"
                     /protein_id="WP_000160316.1"
                     /transl_table=11
                     /translation="MTKGYKFTKEELVEAWKDPQVREKLKDLPKHPSGKALNELSEEEL
                     AEIQGASDVQPETTPLCVGVIIGLTTSIKICK"
     CDS_motif       complement(17854..18006)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     MTKGYKFTKEELVEAWKDPQVREKLKDLPKHPSGKALNELSEEELAEIQGA"
                     /locus_tag="BK765_RS23990_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(17773..17853)
                     /prepeptide="core"
                     /locus_tag="BK765_RS23990_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="4.20"
                     /molecular_weight="2608.1"
                     /monoisotopic_mass="2606.5"
                     /core_sequence="SDVQPETTPLCVGVIIGLTTSIKICK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MTKGYKFTKEELVEAWKDPQVREKLKDLPKHPSGKALNELS
                     EEELAEIQGA"
                     /leader_location="[17853:18006](-)"
                     /alternative_weights="2626.1"
                     /alternative_weights="2644.1"
                     /alternative_weights="2662.2"
                     /alternative_weights="2680.2"
     gene            complement(18038..18271)
                     /locus_tag="BK765_RS23995"
                     /old_locus_tag="BK765_22795"
     CDS             complement(18038..18271)
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002103816.1"
                     /locus_tag="BK765_RS23995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22795"
                     /product="mersacidin/lichenicidin family type 2
                     lantibiotic"
                     /protein_id="WP_000160316.1"
                     /transl_table=11
                     /translation="MTKGYKFTKEELVEAWKDPQVREKLKDLPKHPSGKALNELSEEEL
                     AEIQGASDVQPETTPLCVGVIIGLTTSIKICK"
     CDS_motif       complement(18119..18271)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     MTKGYKFTKEELVEAWKDPQVREKLKDLPKHPSGKALNELSEEELAEIQGA"
                     /locus_tag="BK765_RS23995_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(18038..18118)
                     /prepeptide="core"
                     /locus_tag="BK765_RS23995_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="4.20"
                     /molecular_weight="2608.1"
                     /monoisotopic_mass="2606.5"
                     /core_sequence="SDVQPETTPLCVGVIIGLTTSIKICK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MTKGYKFTKEELVEAWKDPQVREKLKDLPKHPSGKALNELS
                     EEELAEIQGA"
                     /leader_location="[18118:18271](-)"
                     /alternative_weights="2626.1"
                     /alternative_weights="2644.1"
                     /alternative_weights="2662.2"
                     /alternative_weights="2680.2"
     CDS             complement(18303..>18577)
                     /codon_start=2
                     /inference="COORDINATES: protein motif:HMM:NF028244.4"
                     /locus_tag="BK765_RS24000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_22800"
                     /product="mersacidin/lichenicidin family type 2
                     lantibiotic"
                     /protein_id="WP_256942095.1"
                     /transl_table=11
                     /translation="FVNKNKKQNERMNEMTKGYKFTKEELVEAWKDPQVREKLKDLPKH
                     PSGKALNELSEEELAEIQGASDVQPETTPLCVGVIIGLTTSIKICK"
     gene            complement(18303..>18579)
                     /locus_tag="BK765_RS24000"
                     /old_locus_tag="BK765_22800"
ORIGIN
        1 cagaagctgg agctgagatg ataactttct taactgatcc acctaagtgt ttttcagcgt
       61 ctgatttttt agtgaaacgg ccagtagatt ctactactac ttctactccg tagtcgctcc
      121 atggtaattg agctgggtca cgctcagcga taactttaat ttctttaccg ttaacaacga
      181 tgctgttttc gttagcagat acttctgcat ttaaagttcc gtgaactgtg tcatatttta
      241 aaaggtgagc taatgtttta gcatctgtta agtcgttgat tgctactact tctacctcag
      301 agttgttaag agctgcgcgg aatacgttac gtccgatacg tccaaatcca ttaataccaa
      361 ttttagtcat ttgaatttcc tccttggggg attatattaa agggtaaaac cctttgttaa
      421 ctgttttgct gcaccttcat ctgtaattag aattgaagtg tgcccttgtt taattacagc
      481 ctgtattgct tttgcctttg aagaacctcc agcaactgca acaacgtgag atacattttg
      541 taaatcctct aattgcatac caactgttct tactttatga acaacatttc cttcttcatt
      601 aaagtagtaa ccgaaagctt caccaactgc ttcgcttgct tgaatcttta accaatctgc
      661 ttccgaagta cttctgcgac gtgccattgt taacgcatca cctattccat gaatgacgat
      721 attggaagat cgaatcaact caagaacttc tttcacggaa ggctctgtca caatagacgc
      781 atatgcttcg ctactaacat ggtctggaac atacaataag cgataattac tcatcgtatt
      841 ttgcgccatc ttggcacaaa tggtattggc ttctaattca acaccttctc cgattccacc
      901 acgtgctggg acaaatagca tatgtaaatc tttacaatca agttgcatca tgtccgcagc
      961 agcagctagc gtagttcctc cagccacagc aacgatatta ttcgctgtca gacggtcttt
     1021 tatacaagtc acacaagcac ggcccatctc cagtttgacc caaggtgatt catcactatc
     1081 accagggaca acgaaaactt catccaagtc taatgtttcc ttaagttgct tttctaaaac
     1141 ctttaacccg gaaatttctt tcataaagtc ttccaaagca agaactaaag ctgttccttc
     1201 ttctgtcaaa gtcattccag aagaagcgac gtgaactaag ttttgttctt tcaaaacttg
     1261 aacttcactt cgcaatactc gttctgtcat accgaggctt gctgataagt ttcttcttcc
     1321 aatcggctgc atgagcctaa tgtattgaag aatttgcatt ctcgtttgca taacaggtag
     1381 cagatcaggt aataattttt ttgtgttctg aatccatgag cgcatatctt ttctcctcac
     1441 tacattggtt catttttcct cggtggtcac tttacgtccc gtagtgacat tttgtgtccc
     1501 acataggtta aaaaaataat ccctgctacg tgtattattg taacaggaga tagaaactta
     1561 ttcaactctt aactgcattc tttatatagt ttattatgac acctttgtga atgttaccat
     1621 attctacttg ttttccgtct atttctacaa ccggaatcat taaatgatat ttttctaaaa
     1681 ggtcattatc ttcatatata tctatttcct cgatttgaaa agaatattca cattgtactt
     1741 cctgcaaaac ttgtttcgcc tgcacgcaaa gaccacaatt cttttttgta tacagcacaa
     1801 ctttcatctt cttcacctat ccgattgttt ttcgcaatga cgaagctggg atatgcattt
     1861 gttctctata cttcgccacc gttcttcgag aaataacaat ttcatgctct tcttctaata
     1921 attttgaaat cttttgatct gagagtggct ttttcttatt ctctgcttcc acaagcgttt
     1981 gaataaactg tttcactcgc ttcgttgaaa ctgcctcatc ctctgtcgtc gaaactgcat
     2041 tactaaagaa cgatttcatc tcaaataaac cgtgtggcgt ttgcacatac ttatttcttg
     2101 ttgcacgact aatcgtagac tcatgcacac ttagctcttc cgccacctct tttaaagcga
     2161 gcggcttcaa atacgctgga ccttcccaaa agaaatcacg ttgtttttcc ataataatcg
     2221 tcatcacttg cagaagagtt tgttttctct gtttcaaact gcgcataatc cattgcacat
     2281 gctgatactt ttctgaaacg taagatgcta cttcactttc actattatta agaagcgcac
     2341 tgtactcaga gtgaatttca attctcggca tatttctctc attcatctgt aaaacgaggc
     2401 gatcgccttc ttttttcact gccatgtcag gcacaatata cagtggttta tccgaagaat
     2461 acgcaaggcc cggtttcggc tgcaatgatg taatacagtt caccgccgat tgtaattctt
     2521 cattactaca cttcagcaca tgaacgagct ttcgccaatc cttcttcacg aaataagcaa
     2581 agtgctcctc tacaatatct tccgctaatc catcccttct ttgtaagcgc ttcaattgta
     2641 aggttaaaca ttcctgaata ttacgcgccc ctacccctgc tggctccagt gattggacaa
     2701 gctctacaga gcggtcaact acatgaatat gcgcagaaag gagctctgct aactcttcat
     2761 tcgtttcttg taaatacccg tttccatcca tgttcataat aatgaaagaa gccactttcc
     2821 gctcttcttc atctatttta taatactgta tttgatttaa taaatgttgc tgaatcgttg
     2881 tagaatccac actgtagatt tccatctgat tctcgacttg tttgctggta cttttactgt
     2941 tgttagagct ctttttcttc tccctctcaa aaccacctaa ctcaataagg ggattctcta
     3001 acgattgctc atacaaaaac tccgataatt cttgtacatt atactgaagc attgtaatcg
     3061 cttgccttaa ctcttgtgtc attgccaaac gtaagctttg ttcttgtaaa agacttgcct
     3121 tcaaactaat ctcccccttg tttctattgt acaataagtt tggtaaaagg agaatcctca
     3181 cgcagacgcc cattccaaaa tattattaat ttatatgtat taatgatatt tacaacttac
     3241 aaccgaaatt tgaataatat tgaaagaaaa taatagtgcg acaaaaatta ttaccacata
     3301 ctaatagtaa cccttaattt tatatataac aacattaaat ttatctctgt tcaattccaa
     3361 aataaaaaga gcagtttagg tatagtgaca cttcactatg cctaactact cttttacgat
     3421 tctcaattaa gaaagaaacc tcttatttaa agctaaggaa ctttgattcc taattacata
     3481 ggaatcatag agataatcgt tcgattcact agacgctcct tgaattcctc tcatctcttc
     3541 cacagacaac tcaactattt ttttcacgct tctctcgtta ttactatcca tcattttttg
     3601 ttttcttttc gaaaaaaacc ccataattta ttcccccctg caattaatta ttttttaaca
     3661 attgaatgaa tttaaggctc ttaccacttc ttaatacacc tttcggatag accttcgctg
     3721 caaacatagc aattaagcta atgctagcta gtaatacaac catagatgta ataatttcta
     3781 aactcccaac ggatccatta ataatcctaa ctaccatact aattggagct gtaaacggaa
     3841 catatgatgt cacccttgat actagccctt cagggttatc aagtactact aataactcca
     3901 ccactaagct tcccatcaat ataagagtaa tcggcatcgt tgttccggat aattcttctg
     3961 gcctgcttac catagaacca aacactgcga aaagtgccgc ataaacgaag taaccgagaa
     4021 caaaatataa caagaaatat gcagcatgct cacctgtaat catatttaca tcaatattca
     4081 ctccaaaaaa cgaatccgat accttaaaaa ttccagattt aatatatagc gccatactaa
     4141 ggaagaaaat aaataactgt gtcaaactag caagaccgac tcctaatatc ttcccaaaga
     4201 ccatgtgaac aggcgacacc ttcgttacca tcacttccat aacacgcgac gccttctctg
     4261 acgctaccgc tgtagcaatc gcatttccat acgaagtaat tgctaagtac ataatcgcta
     4321 ctaataaata caccagtaag aatgagcttg tttcttcgtt atttaagttt tgctttttcg
     4381 cttgcacgtt catcgtcagt gatttcacag cctcttctga taactgttgt ttaactaccg
     4441 cactttgaaa atactgattt tgtacaaata cctgcgcact ttgaatcaac ttaggattat
     4501 ccaccttcat aaagtaatct aatactggtt cagcacccgc accctctttt aatataaaga
     4561 gcgcttcgac tttcttatct aacaaatctt gcttatattg atttaatttt gttttatcac
     4621 ctaaagacca atcccattct ggaagagctt tactaagatt agattgattc aaagtaacat
     4681 taggtgcttc ggaaataaat acaacttttg ctttttcttt ctctccagaa aacttttgta
     4741 ctaaagttgg aaaggagaat aagaatatcc ctccccctat taataacaat gtcatcccaa
     4801 taaatatttt cgtcctcaca cgatcccaat acgtttgccc taaaacaatc cagaaattcc
     4861 tcattactta tgcacccgcc ttttcaacaa agatttgatg taaagaaggt tctttaatag
     4921 aaacatctcg gatatattgt ttagttttaa tgtcttcaag cagcgaaaat gcatcctctc
     4981 catcattaac tttaatcatg aacgtacgat taactttttc aaaattgaaa tttttattct
     5041 taagaatagg ttcaatatcc ccttccgttt gcaacgtcat atatttgtat tgataacttt
     5101 tcttaatatc tgacaaggca cccgataata caagttgacc atttttcata atacaaattt
     5161 cctcacagaa cgtttctaca ctgtccattt gatgactaca gaagataatc gtttttcttt
     5221 gcgcgattaa ctcgagcaca acatttttta acatctctgc atttacagga tccaagccac
     5281 taaatggttc atctagtatg atgaattccg gattatgcaa tatcgctgaa atcaattgaa
     5341 ttttttgttg atttcccttt gacaattcct ctgttttctt ctttaaatgt ggtgtcattt
     5401 ctaatttttc tagccactgt aacgcatgcc tttttgcaag attagctggc attccttcta
     5461 acttgccgaa atacaccaat tgctccacta cattcatttt cgggtataat ccacgctctt
     5521 ctggtaaata gccaatagat aggtctttct ttgaaaaagg gcgattattc catagaattt
     5581 ggccgctatc cggtttgatt aaatctaaga tcatacgaat cgttgtcgtc ttaccagcac
     5641 catttcggcc cagcatccct aaaatcccac cttcttctac acgtatattt aaatcattaa
     5701 cggctttata ttcaccaaat gttttattta atccttgaat ttcaaacgcc atatataaca
     5761 tcccctttac tgtttaataa agtaaataac tgccattcct aaagcaattc cgccaatcat
     5821 aagtaaaaac tttttaaaat tcatctttat ctctcccatt ttttgaaatt tgtatttcga
     5881 aaatcaagtg cacccttcgc atttacaata ccttttccaa aatatttttt tccgtcatct
     5941 aatgccggtt tggccccctg atataatctt gttttaacaa atccattact aggcttcagc
     6001 ctaaattttt ctttatattc cgcaattaca agtgcggctg tagcagaaac tttaggaact
     6061 gctagactcg ttccaatcat cagctcatac cctctatcaa accctaacca ttcacttagc
     6121 tgggattgcg gaaggtttgt aggatatgtg gttaatacca tagctgtttc atctcccact
     6181 ttttcacttt cccacaaaga tccataatct cctgatggag ctgcaatatc aacattggtt
     6241 ccataattag agtaataagc taatcgatca tctttatttg ttcccgcaac agttactacc
     6301 cccggtaaac ctcctggcat atgaagttgt aagtcgtttt catatcctct ttgcttagct
     6361 aattgaaaag gatttgaaat atcgaatcct tctgttccag aggaagccac tagcaaactc
     6421 cctttactat tcgcatactc tattgctctt ttataagcta agtacacagc tccttcttct
     6481 ggatctttaa tggacttgta tgttcctaaa cttaaattaa taacatccat accatcattt
     6541 gcagcctcta caatagcttt aataatccag cttgaatccg cagaactacc ttgaaatact
     6601 ttgtatggta caatcccaat tttgggcgct acccccttta ttttcccatc agcagctatt
     6661 aacccagcaa tcattgttcc atgaccaata ttatcttcaa ttgattctac attcggaact
     6721 aaaattttcc cctgactaac tatatttccc tttagcgcag gatgatttac atcaatccca
     6781 ctatcaataa caccaatctt tacactatga tttccttgtt caattttata gctctctcca
     6841 ttttctgtta ccttatcgac atcccatttc catttgctat aatcgacctc ttcttgttct
     6901 aattgtgaag gtgcgtattt tgttatattc gaaggtttct ttactatagc gggcggcttt
     6961 aattccacta catcctctct acctgctttt tcaattatcc ctttataatt ttttaataaa
     7021 tgctgcttcg cttcttgtac tctttctaaa ttatcacctt cgatatttag catcttaatt
     7081 tcttttatta cttctagctt taaatcggga tatttatctc taatttcctt tatcatctca
     7141 tccgtatttt caccttgcct caatgtaaac acatacgaat tttctttaat ttgattatgt
     7201 ttcatttctt cagcgcttat cgtactaatt ccaactatag gaaggtttaa taataatcct
     7261 actgtaattc cagcacctat tttcgctata gtgtttcgat atttaggcca atctttcact
     7321 tttaatccct ccttttactt cctgagcttt tgacaattga taataatacc cttgcttctc
     7381 aagtagttct gtatgtgatc ctatctctac aattgctccc ttatccataa caataatctt
     7441 atctgcattc ttaaccgtac ttaacctatg tgcaatgaca atccttgtac ataacatatc
     7501 agttaaattc tcatcaataa ctctttcaga caatgtatca agtgcacttg tcgcctcatc
     7561 taatattaga atagatgggt ttgttaccaa tgctctagct aatattaatc tttgcctttg
     7621 cccaccagag aagttaactc caaattcaga taccatcgtt tgaaaattta atggtagcat
     7681 cataatatct tctaatatat tcgcttgctt cgatgcctca attactccat tcggaggcac
     7741 ccctttcctt tgcgcaatga tattttctcc taccgttttg ttaaacaatc tcgcttcttg
     7801 gaaaacaacg cctattgatt tccttaaaaa tttcaaattt aacttttcgc tttcaatccc
     7861 atcaaatcgg attttcccat tcgttggtgt atatagtccg agaagcattt ttacaattgt
     7921 actttttcca gacccagacg ccccaacaat tgcgaccttc tcaccagctt tgattttaaa
     7981 cgaaacattc tgaattgcat cttcactgaa ataattgtga cgataagaaa cattgtcaaa
     8041 ctcaatatca ccattcagtt tcttaattcc tgaaaattga ttgacctctc tctccgatct
     8101 cgcatgcatt acatcgtaaa tccgctgaat ataggagcta atataaatca attcaccata
     8161 gccactccca atggaaataa tcggagttat aaacgaaatc gctaacgcat taaagccgag
     8221 tacacttcct aatgtcatat ttccagctag cacctgttgt ccactgaacc aaagcagaaa
     8281 caacggtaag ataaattgaa tagaaccggc taatgtattc atcaatgccg tccatataga
     8341 tctcttctct gcatgaataa gctgagaacc aaagcgttct tgccattctt tatacacttg
     8401 cttttccaat cccataacct tcacgtcact aatcccatta atactctcag acaaaatcct
     8461 ttgaacttga gcttgagccg aaacctcttt atcagctaac ttcttagaaa cagtcgtgct
     8521 catgacaagt agggcgaata taacactccc aactgctaac acaacgaatc ctaataaggt
     8581 cgaataacga aacatgaaga ataagtaagt gaaaagcaga attccatcta taagaaagga
     8641 aattacctta gtagataata tttgtctaat atatacattg gaatttgctc taaataacag
     8701 ttctcccata gagcgattct caaaaaagtt ataagaaaga cttaatagtt tttctataaa
     8761 actttgcatt aaagacatat caattgccgt ttgcaacttc gctattagga aacctcttaa
     8821 cgaagcaaat acttgataac ttattagcaa tactaaaatg ctgtaaccta tcatattcat
     8881 atagctcgga tctctcggaa taattacatg gtcagtcatc caacttgtga caaatggaat
     8941 caccagcgca aaagcctgaa ttatcaaaga ggctaataca attaaagcta ttaatttctt
     9001 tttccctaac acaaacgata aaaagaattg caaatgagag acaccttttt gtttttgaaa
     9061 tgactcatct ggttctagtg ttaatacaaa tccagaatac ttttcctcca tttctttttt
     9121 agagatttta tttcttccgt aagcagggtc tataactata gccccctttt ttgtcacttt
     9181 ctctaggact acataatgtt tatgttccca atggataact gctggtaatt taacttcact
     9241 taaagcttca tattcagctt tatatccttt tacttgaaaa ccatttttcc tagcaatatg
     9301 cataagctgg tataaagatg taccaccttt cggaacacca aactcttctc ttaattcagc
     9361 taacgaaaca tgcttttcat aaaaactaaa gaccatcgcc aatgaggcta atccacattc
     9421 gctatgttcc atctgttcta taaagggcac ctttgccatt ttattccccc ctatctcttt
     9481 aactattaat catagcaaca ccctcatggt taccatgatt aaaattaaat tcaaatgtaa
     9541 ttaatcctaa aaaatatgca gctattccaa tttggaccct tgaatgtatt tctaactttc
     9601 ttttaattga ggctactaat tcccccactc tccttctact aataaataac tcccttgcta
     9661 tttcaacatc cttcaaacct cgtgcaatca acacagctac ttctttttct cgaccactta
     9721 aaactttctt catcattctc ctcctttatt ctcaatccat attagattat tttttctcgt
     9781 ttattaaact gtgttgtctt aatctgtact catatcatat ccttctttta aacgttattg
     9841 tccgcaatgc acgttgtagt taccgcaaac aaattagcgg gattctccgc aacatcttca
     9901 taaaaaaaac atatcgcatt tgcgatatgc ttaattgtga agatgtccct cttatgttac
     9961 aatttgaata ttccgaattt ttacaaaagg ggagctgcga gtgatttatt cattacaaaa
    10021 tgaattaatt ttgcgtgcaa aaaaactggt ttatggacat tttttcgaat ctacattacg
    10081 tcaatgttct tcagaatata ttgaatacaa attcaatgat tctactcgtt ttgctaacat
    10141 cactctcgta cattatgaaa tatttcaaga taaccaaaat gaagaggaaa aacaattact
    10201 gctaacaatt atagaattac ttatgctcgc tctagatatt atggatgata ttcaagacga
    10261 agatttctta gaaggacctt ggacaaaaca tccaattgcc acaaatctca atataattat
    10321 gggattttta cttatttgtt tgcaagaagt tgacaaggct tccctttcaa ttcaaatgaa
    10381 aaactggctc aaaaatgaaa tacactcttc tattttaaac tctattaatg gacaacaact
    10441 agatcttaaa aatgaaatcc attccgagaa agattatatt gatatggtaa ctcacaaatc
    10501 tggatatcta attaaacttg catgcttatt aggaacaggg aatatcaacc ctatccagcg
    10561 ttctttaatt gaaaactacg cactatgtat aggcgttatt aatcaaataa aaaacgacat
    10621 gcgtgatatt atgcaatggg ataagaaaaa tgattttctt aatttaaaga aaactttacc
    10681 aatactatat tatttaaata gtaaaaatga taactttata cttataaaaa agttctttaa
    10741 tcaagagata caatatgatg aattaaataa tcaaacatta gattctttaa aacacatatt
    10801 actgtacggt ggatcaatgg agtactgctc cgttatgcag aacctatatg tatacaaatc
    10861 aaaaggatac atcgaacaac tttcactcag tcaagcaaat aaggataggt taattgatac
    10921 tctaatttag tttacttcca tgtctcagca gctttactaa gtaaagacga tttatttaaa
    10981 gtacctaaaa taatttctaa ttcttgtttt gatataccat caatggctat tgttccttga
    11041 ctacacacat ttttaaattg aatatcttct aatatacgtt caactaattc tctaatcata
    11101 tatcgttcac cccaccaatt tttatagttt tatattatta catattcttt tgctattatc
    11161 cgcaatccta ttgcgggatt ccgaaattat atatgatagt gaggtaattc tagcaatgat
    11221 taaacaacgt ccaatttatt tcgtaatttc attaataatt ttttacttaa tcatacaaag
    11281 ttacatcctc tacaagtttt atcagcctta cagtggcatt atcgcttcta aagaaaaaca
    11341 taatacatat acaatcagtc aaattgatac gtcgggttgg gctgcaaaaa cagccttaaa
    11401 agcaggtgat gaaatttaca aagtggatgg ggcgaatcct tatttagggg ttttcaagcg
    11461 ataccaaaat caatttttaa atgcaactga aattcaaact atacagaatg ataggatttc
    11521 tacttatagc acaacagcta cttcagacaa cgaagagcta attattacat tatatctacc
    11581 aatgattttt tctctgacca ttttaagctt atgctttatt gtctatagaa ataaagctat
    11641 aaggcgagaa gagcgttatt ttatgctttt tttattagca acctctatta gcctttccgc
    11701 ctcttctata tctagtaaat ctgattttgg cggactctta attataagta tctcttttca
    11761 aagtgtatct ttatttctta ctttattttt atatcagctg tttaagcgga aggaaattat
    11821 tattatacat aaaaatgtga ttagatttca catttttcta tacatgaccg ttattactac
    11881 cggcttatta tccgttacct ttcttccaaa tttgcaacaa gtctctgctt acacagattt
    11941 agcttattta tgttttagta ttcttttaag tgtatatttc cttttaaaag gatacataaa
    12001 atatagaaat actttacaca ggcctttttt taaatttatg ttagttatcc aatgtatttc
    12061 tttcgggcca tttataatgc tatatgctct tccaaggata ttatttaatg cagccattat
    12121 tagggctgat attgcagcat tattcctatt ttgcattcca attggctact tttatttaat
    12181 attaacaaag caaatctttg atattgattt tatgtttgat cgtgttcggt actactcttt
    12241 gctttcatta cttccaacta tcattattac attaattata agctggggta ttaatcctga
    12301 tgagcgattc tttattagac tcatgcaaaa tttcattatc attttccctt taaacatact
    12361 gtttcttgta ataaaagaac gattagattt cacatttcgc aatcaattat tcagaagcaa
    12421 aaccaattta catgtaagta ttgatcaatt cgcacaacaa ctttcttcca ttatgaaagt
    12481 aaaagattta gagaaaaaat ttatgaatga aattatctcc ctactaaaac cgtgttttat
    12541 acaatttatt gagtttgata caaaacaaaa tacatataat tgttataaaa gttacggaga
    12601 aacagaaaaa tttaatcttt cgaaaaagaa acaatgggga atggaatctg ggtatataaa
    12661 tgatttatta gaagatgacg gatgcatcgg tatttattta tattctaccc aaaacacaaa
    12721 gcattacatt tgggttggaa ataaaaccaa caatattaaa tttaatatca atgaaaaaac
    12781 ttggtttatt gctatcgtaa aatatgtacg tctagtttat gaaaacctaa attcagttaa
    12841 tcaaatcatt caatctttag aaaaagaaag catagcaaat aaaacacact cagcaactct
    12901 ttcgcgccta ctatttcaaa tctcagaaac tgaaaggcgt cggcttgctg cggatcttca
    12961 tgacgctgcc ctgcaagacc aaattgtctg gtatcgtaaa ctagaaaccg tattaaaaga
    13021 acatactgta ccaggtgaat tgcagggaca attagaaaaa ataaaattcg gtatgtttga
    13081 tgttattcaa caaattcgtg acacttgtaa tgaattaaaa ccggctcttc tatctgaaat
    13141 tggtcttata ggtgcactta aagagctatt agcacaaatg caactacgta ctaatttccg
    13201 aattcaattt gaatatgact caatacagga gaatcattac gaatttgata aaattttatc
    13261 tatttatcgt attatccaag aattattaaa caatgctgat aagcactcta atgcaacctt
    13321 aattaatata tttttatggg aagaaaataa ttgtatttat ctagattatc gtgataacgg
    13381 catcggtttt aatacccaag ccccttattc ttcaaataaa catatgggtt tatctggaat
    13441 caaagggcgc gttcaaggaa taggaggcga aattcagctt atttcagaaa taggaaatgg
    13501 actacaagta aatattacta taccgaggtg aataaaatga tacacgtttt gattgtagac
    13561 gatcaccaaa tagtgggtga aggtacgaaa aatatgatcg aattagaaga agatattcaa
    13621 gcaacataca ttttaaacga acaggaagca atttctcaaa gttcaaaaac accttttgat
    13681 atatatcttt tagacatgaa catgcctaat tatagtggac ttgatgtggc tcaaaaactt
    13741 ttacacatac aaccagatgc aaaaattatt ctctacacag gcttcgaata tttatcacaa
    13801 tttaatttat taatagattc tggtattagc ggtattgtta gcaaatcagc atcacagcaa
    13861 gagttattaa tggctattcg ggccgtctta aatgggtata ctttaattcc aatttcttta
    13921 ttgcgacaac ttcgagttga agatatgact gttgataact tagatacgaa aagagcatca
    13981 agccatgtta caattacaca aaaagaatta gaaattcttg aatccattgc gaaaggaaaa
    14041 agtaataaac aaattacaga tgaattatat atgagttcta gggctgttga atataattta
    14101 acgaaactgt ttaaaaagtt caaggtgagt tctcgttctg aagcattagc tgaagctgtt
    14161 cgaaaaggta taataaatgt aaatgtcttt tcatttatcc ttttacattt tctaatgtca
    14221 taacggatgg aatttcattc ggctgtaata gtcttaacaa ctgcatacca actcctgcta
    14281 tccctacaaa cataccaaat gattgaatat catctggtgc atcacattga taactaccag
    14341 tttctctata attattaata acttgtatgc caatttgtaa cgcctgttca tacaattctt
    14401 tattaccata caactgtgaa acagccaata gtaattccgt gtcccccata ttcccatgac
    14461 ataatgtatc attattttta aatccatact ttcgtacatt atctacagcc actcgaattt
    14521 cttcgtccat ttctacatct tcttcatagc ttcttactaa aactctactc aatcctattc
    14581 cagtggaacc gtggcaccat tgatgtaagc acttcttctc actctctctt gtgtctttcc
    14641 acgcactttc ttctttttca aacaatgatc gatcataagc aaatgctttt ttagctaaat
    14701 caatatattc ttgattccta ttcataatcc ctgaacgcca taagctccaa gcgataccac
    14761 tcgtcccatg agaaaaaccg cctaaataac tgttagtatg tttattcttc cacgctactc
    14821 cactttctaa tattgataca ttttccacca aatgcttact gtacatatca gcaagatcta
    14881 aataatcttg atcctttgtc atttcaaata aattaagagc cacttgaata actcctgctg
    14941 agccacttaa caaatcaaac tcttcttctt ctttgagcct ttcttttaat tctactttta
    15001 tttcttctaa aaaatcctga tattcttttc taggacttat agattgaaaa tgtgctaatg
    15061 gatataacaa tgaatattta cccaagaagg tcgatagaag ccccttcgct ttcgataaag
    15121 ggttcatcgc tgacttcaac gccttatgag caacctctaa atatttttcg tttcctgtaa
    15181 ccttatttag atacaataag aatagtgcta cgccacctaa accgtcatat aaattttgct
    15241 tcatcggggc aacataccac ttgtcgtgaa taacatttgc ttgtaaccat gatgctgttg
    15301 ttttatcatc tgataatact acattctcta ctaaagactc tccgatattt atagcttcct
    15361 gcaagattgt ctcctgatga ttttctaata attcacttct gtaatcctct tttcttctca
    15421 ctttaatatc aatatctcta tttccctgta ttgccaactt cagccaccct cgttgctttt
    15481 ccaattcctt atcatcaaaa tgataaattc tattatatac ttgctcataa ccgctctcct
    15541 taaaaaagtc ttccatcctt tcacctgtac tactatatag atctcgtgaa tctgtaaaag
    15601 tcgtaaatat aggaacatcc gcacgtaata agtcttgaat ctcatgaatg actagcccct
    15661 tattttcata tgggtaatac catactcttt caaataattg ttccaataaa attgaatctc
    15721 tcatatagtc tggatgtgta gactcttgta agaaattaca atattgttga gtcgccctag
    15781 caacaatgcg aattttattt tctctaaatg taattaaagg tcctctttct gataaaatct
    15841 cttccttata ctcaataaaa aatttacaca tttcctgaaa accatcaata ataaaggtta
    15901 aataatcctc tacataaatt tcttctactc caagtttcgg tagattttta gcactttgac
    15961 ttttggcttc tttcatagta aattccattt catccttact attttgactt aaggccagca
    16021 ccttaaacgg caaatcttgc tcttttccat ttaaagcact taaatctata ccttttccat
    16081 cgatgttttg aaacatagct tgcggtaaca atccagaccc tatcactgag tcgctttgtt
    16141 catatttcaa ttttacttcc gcactatctg gaaaattcag ttttggatat tgatgaaaaa
    16201 tcgtctccga atcaataatg tatggatact ctccatgagc aattatattt tcaaaatgca
    16261 aatcagcacc ttttaacata tgcattaaac caatcaactt accaaaacgt aaatagtagc
    16321 gctccacctc ttctttagaa gagcacggtt tatacaatat tttttcttcc catccatatt
    16381 caccaaagtc taaaacttta tatgatggaa gttgtatact tccacttacc tcattcatcc
    16441 attctaaaaa tgcatggtac gatttcgtaa tttccataga ctttggctta tataaaattt
    16501 ctttacctga agagaataca agctggacaa ctgttttccc ttcttgatgt gtatctccta
    16561 atccaacctt aatttcgctt aatgatacat cctgcaattg aaattctttt tgtaactgta
    16621 gccaattttc attcaaacga aatagtaaca tttgaacatt ttgtataaaa tactctgttc
    16681 tcaaaattaa ccttcgtgtt aatgttgcgt attcctcata gaacgcaatt aaattctcct
    16741 tatttctagc tttctctctg acaaaggaaa gaaatcgctc ttctggagtc tctcctacta
    16801 gttcttccct tagttttgca atattaaatt ctaagactag tgatcgcacc gcattattta
    16861 ataactcttc tactaaagat cgcactagca tttctttaat ctcttcatgc tcataacgta
    16921 tcctatttaa tctatatttc aacgaactat ctatcatttc ggctgcataa agtgcaaacg
    16981 gcctaacaaa ttctataaac cctaaatctt cctctgcact taatggctta ctttcacaca
    17041 actctattcc tatttctaac acgcgcaaat tcacatgttc atttaattca tctatatata
    17101 aatgctcttt tgattcaggg attttctcaa ttaaactcgc aaaatcttct tttgtcattt
    17161 cattcatttc taatttaaaa tcaaaatcag ctttattact tctgaacatt ttatctatcc
    17221 atttatttaa ttgggctaaa tttaaatcct ccgataataa taaatctctt ctactcttta
    17281 gcgttttata acgctctttc aaattaagac tcttcgcaaa tgccttttca tttatatatg
    17341 agtgaattaa cataataatt taatcccctt ccttatttta aaatccatat tccaataaat
    17401 tggtcccctt caggggaaac agattccaac attcgatttt tcgcattcga atattggaat
    17461 ctgtttcatt caaaaggaga tgaatacccc tttcgaatga aacctatcta cttacaaatt
    17521 ttaattgatg ccgttattcc tataattact cccacacaaa gtggtgttgt ttctggttgt
    17581 acatccgatg ccccttggat ttccgctaat tcctcctcac ttaattcatt tagcgctttt
    17641 ccagatgggt gtttcggtaa gtcttttaac ttttctctta cttggggatc tttccacgcc
    17701 tctactaact cttcttttgt gaacttgtat tcttttgaca tttcattcat cctctcgttt
    17761 tggtttttat ttttatttac aaatcttaat tgacgttgtt aatcctataa ttactcccac
    17821 acaaagtggt gttgtttctg gttgtacatc cgatgcccct tggatttccg ctaattcttc
    17881 ctcacttaat tcatttagcg cttttccaga tgggtgtttc ggtaagtctt ttaacttttc
    17941 tcttacttgg ggatctttcc atgcctctac taactcttct tttgtgaact tgtatccttt
    18001 tgtcatttca ttcatcctct cattttgttt tttattttta tttacaaatc ttaattgacg
    18061 ttgttaatcc tataattact cccacacaaa gcggcgttgt ttctggttgt acatccgatg
    18121 ccccttgaat ttccgctagt tcttcttcgc ttaattcatt taacgcttta cccgatggat
    18181 gtttcggtaa atcttttaac ttttctctta cttgtggatc tttccaagcc tctactaact
    18241 cttcttttgt gaacttgtat ccttttgtca tttcattcat cctctcattt tgttttttat
    18301 ttttatttac aaatcttaat tgacgttgtt aatcctataa ttactcccac acaaagtggt
    18361 gttgtttctg gttgtacatc cgatgcccct tggatttccg ctaattcttc ctcacttaat
    18421 tcatttaacg ctttacccga tgggtgcttc ggtaagtctt ttaacttttc tcttacttgg
    18481 ggatctttcc atgcctctac taactcttct tttgtaaact tgtagccttt tgtcatttca
    18541 ttcatcctct cattttgttt tttattttta tttacaaat
//
