LOCUS       NZ_MOOT01000060        10330 bp    DNA     linear   CON 20-MAY-2024
DEFINITION  Bacillus thuringiensis serovar dakota strain BGSC 4R1 contig000060,
            whole genome shotgun sequence.
ACCESSION   NZ_MOOT01000060
VERSION     NZ_MOOT01000060.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis serovar dakota
  ORGANISM  Bacillus thuringiensis serovar dakota
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 112951)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Comparative genomics of Bacillus thuringiensis reveals a path to
            pathogens against multiple invertebrate hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 112951)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, 1st Shizishan,
            Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.5.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002148095.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 01:21:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,175
            CDSs (total)                      :: 6,055
            Genes (coding)                    :: 5,836
            CDSs (with protein)               :: 5,836
            Genes (RNA)                       :: 120
            rRNAs                             :: 4, 7, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 7, 6 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 88 of 219
            Pseudo Genes (incomplete)         :: 138 of 219
            Pseudo Genes (internal stop)      :: 66 of 219
            Pseudo Genes (multiple problems)  :: 56 of 219
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:21:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 10418
            Orig. end                         :: 20748
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOOT01000060.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10330
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10330
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10330
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5330](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5330)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(170..1321)
                     /locus_tag="BK765_RS23110"
                     /old_locus_tag="BK765_21950"
     CDS             complement(170..1321)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265310.1"
                     /locus_tag="BK765_RS23110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_21950"
                     /product="AimR family lysis-lysogeny pheromone receptor"
                     /protein_id="WP_000265314.1"
                     /transl_table=11
                     /translation="MWKALNQIEKELCAAGIRKNKLANYWGVKPSTVTKVFKGNTDMSF
                     GFLSKTVILLNKGIQVQENLLTDYIYITKPKSENLREAMEDLALRGKFNLLINIINSES
                     QSKVAENREFANVYRIIYRRYIGEIDATQYHKALSLESKSIRTSEMEVLIEILLCQAQY
                     QSGNFTSLNERLKSLEIKINKISNRYIRECYKLRYKEAIAVTSLQGGEVSEARHFSMEL
                     LDDLEWDNFFSFPKVNAYLKLGESYIFSANEYEKAKYYLEKTLEVIGDSKVNGIERKRK
                     MVQHTLSFLKIHHDKGISSLDVVHSGELAYLRIKQGKKIEARKLLNQLKEKNGRLTDIQ
                     TAYLGLTYEGIQKEELMKRSLLMCQKSGNIFYSNLPKIHLGLI"
     gene            1866..2975
                     /locus_tag="BK765_RS23115"
                     /old_locus_tag="BK765_21955"
     CDS             1866..2975
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1077: phage integrase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675870.1"
                     /locus_tag="BK765_RS23115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_21955"
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_086398374.1"
                     /transl_table=11
                     /translation="MKGHIRKRGNKYCIIIDIGPDPETGKRRQKWFSGYKTKKEAQADV
                     AKKITELNEGTFIEPSKVTLKDYLNHWLEIKSMSIQKSTFAGYRAFINQHVIPSIGMVA
                     LHKLNVMHIQKCYKTAINKGITNNSILLMHRILKSALNLAVKQNIISRNPADFAEIPKK
                     EKTSIQTWTEEEVKKFLAHSQESRYHIGYLLAITTGMRLGEVLGLRWQDIDFEKHTVTI
                     NQTSGHDNKIKKTAKTNSSKRTIPVPNETIVALKKHKILINKEKLRFGSAYLDQDLINC
                     NEFGRIIKRAHFRKSFIRATHKVGIKEIKFHDLRHTHATILLKQGVNPKIISERLGHTD
                     ISMTLSVYSHVLPNMQEEAVKNFGKSIFG"
     gene            complement(3014..3715)
                     /locus_tag="BK765_RS23120"
                     /old_locus_tag="BK765_21960"
     CDS             complement(3014..3715)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736205.1"
                     /locus_tag="BK765_RS23120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_21960"
                     /product="DUF3962 domain-containing protein"
                     /protein_id="WP_000736198.1"
                     /transl_table=11
                     /translation="MKKLRLLTFENIVEPLLNEKVSFIYFPIEWLDIVEIHYKTFLLTS
                     KLKRLNERLYDMFSDILFIQHNPYVLNENTPWIVSKEPIKQEQLNYIFQSWYEIIHDWK
                     PNKLVEPPKYEWQSDLISNLPVLHDNETYSKWVPALISHIFCERPIYLENTNAEEIYFS
                     PLRSQNICEAMSGPIKDEKTQDFFSYVYRFECITRGGENAPLLNISIGIRRFYQEYKMI
                     GQTNLDMTTFV"
     misc_feature    complement(3707..3720)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.3"
                     /tool="antismash"
     misc_feature    3762..3775
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.22"
                     /tool="antismash"
     gene            complement(4022..4507)
                     /locus_tag="BK765_RS23125"
                     /old_locus_tag="BK765_21965"
     CDS             complement(4022..4507)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124614.1"
                     /locus_tag="BK765_RS23125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_21965"
                     /product="hypothetical protein"
                     /protein_id="WP_002082715.1"
                     /transl_table=11
                     /translation="MNMVELKSIFHSYKVKRKIAKDLYGKRDELTLLLNEFNNMKCTVT
                     SEKKKNNILSRLQLIYQNIKLDKQYPLSVSFDSKLLERLEKESLHTIEDGVTCLHLMLD
                     MNYEKIKQYGSSTSRSFVPLSQSSICLADCICLTGFIVGLLGVISFGGFMLSICSTT"
     misc_feature    4539..4557
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.87"
                     /tool="antismash"
     gene            complement(4717..>4923)
                     /locus_tag="BK765_RS33180"
                     /pseudo=""
     CDS             complement(4717..>4923)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016077325.1"
                     /locus_tag="BK765_RS33180"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="site-specific integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="AAHKSPMDEG"
     gene            complement(5001..5330)
                     /locus_tag="BK765_RS23130"
                     /old_locus_tag="BK765_21970"
     CDS             complement(5001..5330)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071363.1"
                     /locus_tag="BK765_RS23130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_21970"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001071349.1"
                     /sec_met_domain="TIGR03601 (E-value: 5.4e-40, bitscore:
                     128.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQEIQALSLNDYRSGNIVYWDQQNQYPYYYIEDAARRCGGC
                     GRCGGCGGGRCGGFRCGGFRCIGCFGCFGCGGCGGCGGCGGCSNCFDGFSGTTDTTGTI
                     ITYEY"
     gene            complement(5784..6047)
                     /locus_tag="BK765_RS23135"
                     /old_locus_tag="BK765_21975"
     CDS             complement(5784..6047)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101034.1"
                     /locus_tag="BK765_RS23135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_21975"
                     /product="DUF3937 domain-containing protein"
                     /protein_id="WP_002082716.1"
                     /transl_table=11
                     /translation="MNFERCYMFTNKKLIRFGLSLFVFLGIINFTISYFQTYLETAADI
                     KWVIPEIWKTILLDVPQGILVLLGAIALYDFTKEASQKDASI"
     gene            complement(6445..7134)
                     /gene="tenA"
                     /locus_tag="BK765_RS23140"
                     /old_locus_tag="BK765_21980"
     CDS             complement(6445..7134)
                     /EC_number="3.5.99.2"
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="BK765_RS23140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_21980"
                     /product="thiaminase II"
                     /protein_id="WP_000144512.1"
                     /transl_table=11
                     /translation="MTFSQSLRKEVDSIWEASFNHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITKEEKDNFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEQIYNEWISAY
                     GSDWFRMLVEEQITRLDTIAEKVTEADRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDT
                     DVKDVIG"
     regulatory      complement(7262..7367)
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     misc_feature    7406..7418
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     gene            complement(7441..8355)
                     /locus_tag="BK765_RS23145"
                     /old_locus_tag="BK765_21985"
     CDS             complement(7441..8355)
                     /EC_number="6.3.2.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="BK765_RS23145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_21985"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_016098569.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 2.5e-23,
                     bitscore: 75.6, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 2.9e-15, bitscore:
                     49.6, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 5.7e-10, bitscore: 32.0,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 1.3e-54,
                     bitscore: 177.7, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 2.9e-07, bitscore:
                     23.1, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKVD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRY
                     EGVETPDWIELTKMEDLNLDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVF
                     EWDSEVVIEKYIKGDEITCSILDGKQLPIVSIRHAAEFFDYNAKYDDTSTVEEVIELPA
                     EINERVNKASLACYKVLKCSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAG
                     ISYSKLLDMIIETSLKVRKEEGF"
     gene            complement(8360..9808)
                     /locus_tag="BK765_RS23150"
                     /old_locus_tag="BK765_21990"
     CDS             complement(8360..9808)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-469). E-value:
                     5.7e-30. Score: 96.6. Matches aSDomain:
                     nrpspksdomains_BK765_RS23150_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="BK765_RS23150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_21990"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_016098568.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.2e-29, bitscore:
                     96.5, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYLKKMIMKGHLLGDQKIPSTRELSEL
                     LSVSRNTVLAAYADLEQEGLIYAVKGKGNFVAKVDISNNSSVEIDWKNKLNTVTALADE
                     LDLMKHGVRWEKGMIVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYRPL
                     MNYLLHYMEMKGVDISNKDILITNGFTEGLDIVLSSLSKKSGRVICENPTHHAALKLFR
                     LHGLEVHGIDMHEDGIDTNEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYKVPIIEDGFNEELRYSGSHLAPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQY
                     IHFKRMTGDGGLHLFIELEEHMNARTLLQKCYEKGVTFSPGDVFYSDGEGANTFRLGFS
                     RLKEEEIVRGIKIIGDTLKNEIWS"
     aSDomain        complement(8402..9373)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK765_RS23150_Aminotran_1_2.1"
                     /evalue="5.70E-30"
                     /label="BK765_RS23150_Aminotran_1_2.1"
                     /locus_tag="BK765_RS23150"
                     /protein_end="469"
                     /protein_start="145"
                     /score="96.6"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYRPLMNYLLHYMEMKGVDISNKDILITNGFT
                     EGLDIVLSSLSKKSGRVICENPTHHAALKLFRLHGLEVHGIDMHEDGIDTNEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYKVPIIEDGFNEELRYSGSHLAPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQYIHFKRMTGDGGLHLFIELEEHMNARTL
                     LQKCYEKGVTFSPGDVFYSDGEGANTFRLGFSRLKEEEIVRGI"
     misc_feature    complement(9884..9898)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 17.35"
                     /tool="antismash"
ORIGIN
        1 agggtcaacc atcatatgtt gaattgtaga ttttgaatta tttgtactag gcttttcttt
       61 tatagaatca gtgttaatga ataatgtagc agctattaat agcgcaggaa tgattgtaat
      121 tatttttttc attatttcac ctctttccga agacaattat accaattatt caaattaaac
      181 ccaagtgtat ttttggtaaa ttcgaataaa atatattccc tgatttttga cacatcaaaa
      241 gagaacgttt cattaactct tctttttgaa taccttcata tgttaaacct aaatatgcag
      301 tctgtatgtc tgttaatctt ccattcttct cttttaattg atttaatagt tttctcgctt
      361 caattttctt accttgttta atccttaaat atgctaattc acctgaatgg acaacatcta
      421 agctactaat tcctttatca tgatgaattt ttagaaatga taatgtatgt tgcaccattt
      481 ttctttttct ctcaattcca ttaaccttac tatcccctat cacttcaaga gtcttttcca
      541 aataatattt tgccttctca tattcattcg ctgaaaaaat ataagattca cctaacttta
      601 aatatgcatt tacttttgga aaagaaaaaa agttatccca ttcaagatca tctaatagtt
      661 ccatgctaaa atgccttgct tcactgactt caccaccctg caacgaagta acggcaatgg
      721 cttctttata tcgtaattta taacattctc gaatgtacct attacttatt ttatttattt
      781 ttatttcaag agattttaat cgctcattta aagaagtaaa attacccgat tgatattgcg
      841 cttgacataa taaaatttca attaacactt ccatctctga tgttcttatt gatttacttt
      901 ccaggcttaa tgccttatgg tactgtgtag catcaatctc acctatatat cgtctatata
      961 taattctata cacattagca aattctctat tttctgctac ctttgattgt gattcactat
     1021 tgataatatt aattaataga ttaaactttc cccttaaagc taaatcctcc attgcctcac
     1081 gtaagttttc tgattttggt ttcgttatat atatataatc tgttaacaaa ttttcttgaa
     1141 cctgtatgcc tttgtttaat aggatgactg tcttagaaag aaagccaaaa ctcatgtctg
     1201 tgttaccttt aaaaactttt gtaacagtac ttggcttaac tccccaataa tttgctagtt
     1261 tattttttct tattccagct gcacataact ctttttcaat ttgatttaga gctttccaca
     1321 tgttttgtcc cccttattgg aacaagacac actcccctat catgaaaacg caccttaacg
     1381 atgaattaca tctaaaagtt gtgttatact agccgtatat gttacgcata gtcgtaactg
     1441 aaaggctcat ggcaaatgtt ttccctacta caattagggc aaacggtgta aaagtgttcc
     1501 cagcacaatt acacacgcta tgggtctttt tcgttccgtc aaattatatt attaagaata
     1561 ttctatcaca aataacccaa acatctattc tctcatattc tgaaaatact tgagaaagtt
     1621 aaaaatatta atatactcct tgttttttag acttcctaaa aaaatatgca atattgcatt
     1681 tacaacagga atagacattt cactattgtt taacagcaaa tgaaacccct acactataac
     1741 tgaattcatt tttatatggt aaaatatacc catcgctgat atgtccaaac atgtaatttt
     1801 catagcagca aaattacaac tagacttata caacatgatt caaaacaaat gaaggagtgt
     1861 tttaagtgaa aggacatatt cgaaaacgag gaaataagta ttgtattatt attgatatcg
     1921 ggcctgatcc agagacagga aaaagaagac agaagtggtt ttctgggtat aagacaaaaa
     1981 aagaagcaca ggctgatgtg gcaaagaaaa ttacagagtt gaatgaagga acttttatag
     2041 agccatctaa agttacgcta aaagattacc taaatcattg gctagaaatt aaaagtatga
     2101 gtatacaaaa gagtaccttt gctggctata gggcatttat caaccaacat gttataccta
     2161 gtataggaat ggtcgcactc cataaattaa atgttatgca cattcaaaaa tgctataaga
     2221 ctgcgataaa taaaggtatt acaaataatt ccattctgct tatgcataga attttaaaga
     2281 gtgctttaaa cctagctgta aaacaaaata ttatttctcg aaatccagca gattttgctg
     2341 agatacctaa aaaagaaaaa acctctatcc agacttggac agaggaagaa gtgaaaaaat
     2401 ttttagctca ttcacaagaa tcacgatatc acattgggta tctacttgca ataactacag
     2461 gtatgcgtct gggagaagtt ctagggttac gatggcagga cattgatttt gaaaaacata
     2521 ccgttacaat aaatcaaaca tctggtcatg acaataaaat caaaaaaact gcaaaaacaa
     2581 attcgtcaaa acgcacaatt cctgtaccta atgaaacaat agtagcctta aaaaaacata
     2641 agattttaat taataaagag aaattaaggt ttggctctgc ttatctagat caagatttaa
     2701 taaattgtaa tgagtttgga agaatcataa aaagagcaca tttcagaaaa agtttcatta
     2761 gggcgacaca caaagtaggt ataaaagaaa ttaaattcca tgatttaaga catacacacg
     2821 caactatact attgaaacaa ggagttaacc ctaaaatcat cagtgagaga ttaggtcata
     2881 cagatatttc aatgacatta agtgtctatt ctcatgtttt accgaatatg caggaagaag
     2941 cagttaaaaa cttcggtaaa agtatctttg gataacctat gtttgcaaaa tgtttgcatt
     3001 tcatcaaaaa aagtcaaaca aacgttgtca tatcaaggtt tgtttgacct atcatcttat
     3061 attcttgata aaatctccga atccctattg aaatatttaa taatggagcg ttctcaccac
     3121 cacgggttat gcattcgaat cgatatacat aggagaaaaa atcttgtgtt ttttcatctt
     3181 ttattggccc tgacatcgcc tcacaaatat tttgtgatct aagaggagaa aaatagattt
     3241 cttctgcatt tgtattttct aaatatatag gacgctcaca aaaaatatgt gagattaaag
     3301 cgggcaccca cttagaatac gtttcattat catgtagtac tggcaaatta gaaatcaaat
     3361 cggattgcca ttcatatttt ggtggctcta ctaatttatt tggtttccaa tcatgaataa
     3421 tctcatacca actttgaaaa atataattga gctgctcttg tttaataggt tcttttgata
     3481 caatccatgg tgtattttca tttaatacgt acggattatg ctgaataaac aatatatcag
     3541 aaaacatatc atacagtctt tcattcaaac gtttcaactt actcgttaat aaaaacgtct
     3601 tataatgtat ctctacgatg tccagccatt caataggaaa gtatataaat gatacctttt
     3661 catttaaaag aggttctact atattttcaa atgttaacaa ccttaatttt ttcataaaat
     3721 gattctttcc tttcttcagt tgtcttgatt atcaaaaagc tagaacaaaa gttacttaat
     3781 ctaatccatt taaacaaagt atttaaaaga aaagtaaaaa ctatctacat aaaacattat
     3841 caaaccgact cttttaaaaa ttaaaagtaa ttattatcta caacacctct tagtattcat
     3901 aatttaatac atttaaatta tataatacat ataatatatt gtacttatca tttacaataa
     3961 ctttacagaa aaaccaaaaa agaacacctt aaatcggtgc tctttttaca aatatcaagc
     4021 gttatgtagt tgaacatata gataacatga accctccaaa tgaaattact cctagtaaac
     4081 ctactataaa tcctgttaaa caaatacaat cagcaagaca aatagaagac tgcgataatg
     4141 gaacgaatga cctacttgta ctcgacccat attgttttat tttttcataa ttcatatcta
     4201 acattaaatg tagacatgtt acaccatcct caatagtatg tagagattct ttttctaatc
     4261 gctccaataa tttactatca aaagaaactg atagaggata ctgcttatct aattttatat
     4321 tttgataaat tagttgcaaa cgagataata tattattttt cttcttttca gatgttactg
     4381 tacatttcat attattaaat tcatttaata acagtgtcaa ttcatctctt tttccgtata
     4441 aatctttagc tatttttctc tttactttat acgaatggaa aatagatttt agttcaacca
     4501 tattcataca cttcctaatt acatattatt tacccaatta ttttatagat aaacagtact
     4561 ttttaatctt atttaaaata cactcaagca acttatttta ctatcgatta acattacatt
     4621 aaccttacac ggttgtaata aaatcattag ctagaaggaa gcaatgccca aactagacag
     4681 atatttaatg ataaaaccat aagaaaaaaa caatgattag attttaaatc tagtcattgc
     4741 tttatccatc acatcttgat tacacctata tatcttattg ttactctttc acttgaatga
     4801 ttgaatatat ccattaataa tgactatatt ctttgtcggc acgtacatat ataaaactaa
     4861 tgttttttaa ttgataaggt gagactttaa ttagccctca tccatcgggc ttttatgggc
     4921 agccggccac ctaacttctc tttgctctcg ctgaattttt ttagggtctt actgcccggc
     4981 aaatagcagg ataaatgcaa tcaatattca tacgttataa tagtaccagt agtatcagta
     5041 gtaccactaa aaccatcaaa acagttagag caaccgccac agccgccaca accaccacaa
     5101 cctccgcaac caaaacaacc gaagcaacca atacaacgga atccaccaca acggaatccg
     5161 ccacaacgac ctccgccaca accgccacaa cgaccacaac ccccacagcg acgagcagca
     5221 tcttcaatat agtaatatgg atattgattt tgctggtccc aatagacaat atttccagat
     5281 cggtaatcat taaggcttaa cgcttgtatt tcttgttgaa actgattcat tttcataacc
     5341 tccgtttata aaatacaacc tcattgatgc ttccctatat ttctgttcgt tatatctaag
     5401 tagaaatagc gctataaact aagttcaata cgtacaccaa caaagtatga cttatcacta
     5461 gatgatgcac cttgttcata tacctatttt tactatgggc tcatttttat aaatagaaat
     5521 tataaaatat aaattctagc gtcatatctt catacttact aagctcaatt aatttctcaa
     5581 taatcacgcg gacaaatcct atatatttcc atatatcact tagattttct tgtactgtaa
     5641 atgcctttgc tataacacct ctaagtaacc ggtttctatg acggttaaga aattcctata
     5701 aagaaaaaag ccctaattag ggctttttat tctatttctc cagcaaagct ctctatgaaa
     5761 tttctaattg aaaaaagaca cacttagatt gatgcgtctt tttgtgatgc ctcttttgtg
     5821 aaatcatata atgcaattgc gcctaaaaga acaagtatac cttgaggaac atctagtaaa
     5881 atagttttcc aaatttctgg aattacccat ttaatatctg ctgctgtttc aagatatgtt
     5941 tggaaatagc tgattgtaaa attaattatc cctaaaaata caaacaacga taaaccaaat
     6001 cgaattaatt tcttatttgt aaacatataa cacctctcaa aattcattgg atattcctgc
     6061 ttttgcttct ttatatttcc atatctctat atttgatttt aacacacaaa gaactcaaaa
     6121 aaatacaata tgcaaatttg catattgtat gaacagcact tcatgttaac ttagaaattc
     6181 ctgcaaagaa aaaacaactc cgttacagct aacagctcgg gtttcttttc ttatttaact
     6241 tcataccacc aacatttacg ttctttaatt gtaaaataaa tgtgacagaa gtttatccag
     6301 ttaatgcgta gacaattaaa tgagctaaat tatcgatatt ctatttcacc atatccgtag
     6361 cactaatgac tttacgcaaa tttgacaaga aaaaatatag agataggtat gatccatacc
     6421 tatctctaac attacttcta ttctttatcc aattacatct tttacgtctg tatccactgg
     6481 ccacttttct aatgtataag ccatttccca aaatgaatat tcatattgac tgctaataat
     6541 aaaatgctgt ttcatacgct tgcggtctgc ctctgttact ttttcagcga tagtatctaa
     6601 acgtgtaatt tgttcttcaa ctagcatacg gaaccaatca gatccataag cagaaatcca
     6661 ttcgttataa atttgctctt ctggctgaca ctcttttaaa cgctcaccaa tttcataata
     6721 caaccaatag caaggtaaaa ttgctgcaat gatatctcct aaatgccctt catacgcagc
     6781 acgatacata tgtgaagtat acgcatatgc agttggcgca ggaataaaat tatctttttc
     6841 ttcttttgtt atccctagct ttttagcaaa attctcatgt aaagataact cggcctcata
     6901 tgtattttga gcatgatgtg ccatacgagc tgtcgtctct aattcaagag cttttgcagc
     6961 tcctaaagtt tgtactctag caaaatgact caaataatat gaatcttgaa gcacataata
     7021 acgaaaacta gctaaatcta acgttccttc accaagtttt tttacaaaag gatggttaaa
     7081 actcgcttcc caaattgaat ctacttcttt acgtaatgat tgtgaaaaag tcatttaaat
     7141 ccctccaata atgtataaaa tataagtatt tatccacaat tattaaagct gtaaaataaa
     7201 aaaactttct ttacaggcgt aaagaaagtg aaattgacag tatttattta ctcattaaat
     7261 agcaacgtct ttcccacttc cctacgctgg cattaaccag atcaggtact aagagtctca
     7321 aaatcacatt tgatctcagc ctataaaagg cccccctagt ggataaatat tttgttttta
     7381 taatgtattc atcatagcat acttcgtgaa actgtcaaaa gaattcgtta tatgattccc
     7441 ttaaaatcct tcttcttttc taacttttaa tgatgtttca ataatcatat ctaatagttt
     7501 gctataacta attcccgctg cttctgcact ttttggcaat aaacttgatt gtgtcatccc
     7561 tggtaatgta ttaatttcca ttacatatgg aattccgtcc ttcaccatca tatcaactct
     7621 tgcataaaca ctacatttta atactttata acaagcaagt gacgctttat ttacacgttc
     7681 attaatttct gctggaagtt cgataacttc ctcaactgta ctagtatcat catattttgc
     7741 attgtagtca aagaactcgg ctgcatgtcg aattgaaact ataggtaact gttttccatc
     7801 cagaattgaa catgtaattt catcaccttt tatatacttc tcaattacta cttcagaatc
     7861 ccattcgaat acagtttcta gcattgagat caattcattt ttattataaa cgattttcac
     7921 tccgacactc gatccgccag agtttggttt tactactaat ggaaatccta acttatctaa
     7981 ctcatcaaga tttagatcct ccatttttgt aagttcaatc caatctggtg tttctacacc
     8041 ttcataacgt aaaatctttt tcgaaatatt tttatccata catatactgc tagataacat
     8101 attacttccg ctataaggaa tccctaaact ctccagtgtt ccttgaactg tcccatcttc
     8161 tccgtatttt ccgtgtaacg cgagcaacgc aaagtcaatg tctttcgctt tttcaattaa
     8221 atccactttt tcatttaatg taattggaac tatttcatac ttatttttat ctaaatgagc
     8281 aatcatttca ttccccgtca taattgaaac ttgtttttca gacgataccc cgcccataat
     8341 aacaccgatt ttcattttct caactccaaa tttcattttt taatgtatca ccaattattt
     8401 taattccccg aactatttct tcttctttca agcgcgaaaa ccctaatcgg aaagtgttcg
     8461 ctccttctcc atcagagtaa aaaacatctc caggtgaaaa cgtaacgcct ttctcataac
     8521 acttttgtaa aagtgtgcgg gcattcatat gctcttctag ctctataaat aaatgaaggc
     8581 caccatctcc agtcattctt ttgaaatgaa tgtactggtt acatgccccg acagctaact
     8641 catatttttt cttataaaca gatcttgcct tttttaaata tttttcaaaa tatccctcat
     8701 gtaaatattg aaacagaaca gcttgatcta acgtagatgt gtggatcgtt cttgctcttt
     8761 ttacgctttc taaataatga atcagctctt tatctgcaat aatccagcct acacgtaaac
     8821 cagggaaaag tacctttgaa aagctactaa tataaatcac attattgcca gccccagcaa
     8881 aagttaataa tggagctaaa tgtgaacctg aataacgtaa ttcttcatta aatccgtcct
     8941 ctataatagg aaccttgtat tttgagaata acctcatcaa ttccgttctt ttctctgaac
     9001 tcgtaacaat accagttgga ttatgataag aaggaattaa ataagcaaaa tcaaaatctt
     9061 tttcacgcaa acttttttca acttcattcg tatcaatacc atcctcatgc atatcaatcc
     9121 catgaacttc aagtccatgt aaacggaaaa gcttaagtgc agcgtggtga gttggattct
     9181 cacaaatgac acgaccggat ttttttgata aagaagagag tacaatatcc aatccttctg
     9241 taaatccatt tgtaattaga atatctttat ttgaaatgtc tacacctttc atttccatgt
     9301 aatgaagtaa ataattcatt aacggtctat atccctttgc atatccgtag ttcaatacga
     9361 tgtccccttc aatggacata cgagtaagaa aagctctttt gaaattttca acatcaaata
     9421 gcttctcatc cggggctata ctattaaaaa caatcattcc cttttcccaa cgaacaccat
     9481 gtttcattaa atctaattca tccgctaatg cggtcactgt attaagttta tttttccaat
     9541 ctatttcaac agacgaattg ttagatatat ccaccttcgc aacaaagttc ccttttcctt
     9601 taactgcata aatgagtcct tcttgctcta aatctgcata agcagcgagt acagtgtttc
     9661 tactcacact tagtaattca cttagttccc ttgttgatgg gattttttga tccccgagca
     9721 aatgcccttt cataatcatc ttcttcaaat aatctttcaa ttgaatataa acgggacgat
     9781 ctttaacaac tttaaaatcc ttaaacaatt ccttaccccc tgcaatcatt tttgcatatt
     9841 tatttcattc tcaaaaggtc cacgacatat gtatttttta taaagggcgg tacggtctta
     9901 caaatctttt cgatataagt taattacatc ttttaaagct gcagctgcag cgcctcgtgg
     9961 attagaagcc ccaccaattg tagtaacttg tcccatcgta tctgtaaaaa acgtaattcc
    10021 tttttcggtc ccatttactt ttgctaacgg atgatctttt tctatttcgc acggttcaac
    10081 atttaaattg acattgccac cctcatcctt atatgttgat gctattaatt taataatttt
    10141 atgttgttcc ttagcatttc gaatttgttg ttttgtaacg tgctcgattc cttttatatg
    10201 tatatcagta agtgttttct ctgttcccat taagctattt gccaaaagta acaatttaca
    10261 cgcactatct gagccactta tatctaatat agggtttgtc tcagcaattc ctttatcttg
    10321 tgcttctttc
//
