LOCUS       NZ_MOOT01000059        10264 bp    DNA     linear   CON 20-MAY-2024
DEFINITION  Bacillus thuringiensis serovar dakota strain BGSC 4R1 contig000059,
            whole genome shotgun sequence.
ACCESSION   NZ_MOOT01000059
VERSION     NZ_MOOT01000059.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis serovar dakota
  ORGANISM  Bacillus thuringiensis serovar dakota
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 437802)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Comparative genomics of Bacillus thuringiensis reveals a path to
            pathogens against multiple invertebrate hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 437802)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, 1st Shizishan,
            Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.5.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002148095.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 01:21:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,175
            CDSs (total)                      :: 6,055
            Genes (coding)                    :: 5,836
            CDSs (with protein)               :: 5,836
            Genes (RNA)                       :: 120
            rRNAs                             :: 4, 7, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 7, 6 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 88 of 219
            Pseudo Genes (incomplete)         :: 138 of 219
            Pseudo Genes (internal stop)      :: 66 of 219
            Pseudo Genes (multiple problems)  :: 56 of 219
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:21:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 86114
            Orig. end                         :: 96378
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOOT01000059.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10264
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10264
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10264
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5264](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5264
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1254..2168
                     /locus_tag="BK765_RS21055"
                     /old_locus_tag="BK765_20005"
     CDS             1254..2168
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017673335.1"
                     /locus_tag="BK765_RS21055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_20005"
                     /product="DMT family transporter"
                     /protein_id="WP_000551632.1"
                     /transl_table=11
                     /translation="MHNKKWDLRIIYAHAFTILIWGTAFPAIRMGLESYTPEHLTLLRL
                     LIASFILLLFSFIYKLRLPDLKDIPAIFMFGALGFTFYHIALNYGEKTVNAGSASLIVS
                     VTPIVTAILASIFLNEKMKLNGWIGGVISFIGIALISFSQGDAIQLNSGGLFILLAAIS
                     ESLYFVFQTSYLKKYGFLPFTIYTILSSTVCMLIFLPGMYTEILAAPLEVNVSVLYLGL
                     FPTVLPYIALAYIISHAGAAEATSSLYLTPVTACFIAWLWLGEVPTLVSIIGGGITILG
                     IVIAHIPVLRKEKHRNLANNQSV"
     gene            2307..3668
                     /locus_tag="BK765_RS21060"
                     /old_locus_tag="BK765_20010"
     CDS             2307..3668
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080598.1"
                     /locus_tag="BK765_RS21060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_20010"
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_016098589.1"
                     /transl_table=11
                     /translation="MNGKRNIFTCISIIGIGLASFSNSSFAANVTDNSVQNSIPVVNQQ
                     VAAAKEMKPFPQQVNYAGVIKPTHVTQESLNASVRSYYDNWKKKYLKNDLSSLPGGYYV
                     KGEITGDADGFKPLGTSEGQGYGMIITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNL
                     MGWVVADSKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGTVNYLKEAQDMITKGIKAS
                     NVTNNSRLNLGDWDSKNSLDTRPSDWMMSHLRAFYEFTGDKTWLTVINNLYDVYTQFSN
                     KYSPNTGLISDFVVKNPPQPAPKDFLDESKYTNAYYYNASRVPLRIVMDYAMYGEKRSK
                     VISDKVSSWIQNKTNGNPSKIVDGYQLNGSNIGSYPTGVFVSPFIAASITDSNNQKWVN
                     SGWDWMKNKRESYFSDSYNLLTMLFITGNWWKPVPDDKKIENLINDETQKGYDK"
     gene            3862..4329
                     /locus_tag="BK765_RS21065"
                     /old_locus_tag="BK765_20015"
     CDS             3862..4329
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="BK765_RS21065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_20015"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_016098590.1"
                     /transl_table=11
                     /translation="MNIQLKIVTRANWEDAFKLQVKKDQRKFVPSVAVSLAKVYIKPDG
                     DHVEYIPFAIYDGELMVGFVMHAVIKETTDMYWINGFIIDQKQQGNGYGKAALQESVYL
                     IKNTYKACKEIRLTVHKDNISARKLYECYGFQSLGHDYDGEQVYRLFVNQI"
     gene            4355..4801
                     /locus_tag="BK765_RS21070"
                     /old_locus_tag="BK765_20020"
     CDS             4355..4801
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="BK765_RS21070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_20020"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_016098591.1"
                     /transl_table=11
                     /translation="MKTKFHHIVRAVMIKDKKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LIRELREELGVNCSIQQFLGVIENQWQDKEVLHHEINHIFEVESQDLHTDLPPKSSESH
                     LAFQWIDCNKEALNHYEIMPMPLVKELLERKLSDELLNCWISNF"
     gene            5001..5264
                     /locus_tag="BK765_RS21075"
                     /old_locus_tag="BK765_20025"
     CDS             5001..5264
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071342.1"
                     /locus_tag="BK765_RS21075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_20025"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_060486806.1"
                     /sec_met_domain="TIGR03601 (E-value: 3.3e-41, bitscore:
                     134.5, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQSLNLNDYQSGNVVYWDQLQQNQYPYYYIQDDARRCG
                     GCGGCGGRCGGCGGGRCGGCGGRCVGCAGCFGCFNCWNWWII"
     gene            5367..6382
                     /locus_tag="BK765_RS21080"
                     /old_locus_tag="BK765_20030"
                     /pseudo=""
     CDS             5367..6382
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="BK765_RS21080"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_20030"
                     /product="serine hydrolase domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MYTYDKLISWVENIKEENHSSAAALSIIKDNKIVLEHYSGYHSNT
                     STSKKVTASSQFNVASARKSYLGLMVAYALYEGKINSIDDAAIKYVKDFDSALLGKTTI
                     RHLVTHSHGLGETNDGTIFREFEPGQAWAYRDINVRMMTHLIYQLYNKSFPELLKERVF
                     DLANFKETGWRIQQDENLVEIINNPNEDPISGIGTVNDGTEKIYLSQLENLRSGAIFI"
     gene            6414..6557
                     /locus_tag="BK765_RS32565"
                     /old_locus_tag="BK765_20035"
     CDS             6414..6557
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="BK765_RS32565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_20035"
                     /product="hypothetical protein"
                     /protein_id="WP_000653367.1"
                     /transl_table=11
                     /translation="MKDKMNKNKKQKIKDVNTEQNAIYSDPKDAANMQTVPQPKDFDEI
                     EY"
     misc_feature    complement(6559..6579)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 24.59"
                     /tool="antismash"
     gene            6843..8051
                     /locus_tag="BK765_RS21090"
                     /old_locus_tag="BK765_20040"
     CDS             6843..8051
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="BK765_RS21090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_20040"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_086398308.1"
                     /transl_table=11
                     /translation="MKQSSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAAKLQKKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLINNLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNAVTETLHNDYTPREMMRTKQVYLLFFMLFTS
                     CMGGLYLISMVKDIGVQLVGLSAATAANAVAMIAIFNTVGRIILGTLSDKIGRMKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGAILGGFQPTFIIIGVLSVISFIISILIRPPTAEKKKELKHLHR
                     KVA"
     gene            8182..8607
                     /locus_tag="BK765_RS21095"
                     /old_locus_tag="BK765_20045"
     CDS             8182..8607
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706722.1"
                     /locus_tag="BK765_RS21095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_20045"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_086398309.1"
                     /transl_table=11
                     /translation="MKIVQQWIQEDSDYIREKVIEYNQKYLADEEKTPSEKVSFIVRNE
                     KEEIVGGVTAIIFWHHVHVDFLWVSEEYRHEGYGNKLIKLIEEFAIEKGCRLINLDTFS
                     FQAPDFYKKHGYKVIGVSEDHPKGHNHYYLEKRLKSI"
     misc_feature    complement(8790..8808)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.4"
                     /tool="antismash"
     gene            8889..9332
                     /locus_tag="BK765_RS21100"
                     /old_locus_tag="BK765_20050"
     CDS             8889..9332
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="BK765_RS21100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_20050"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_000757601.1"
                     /transl_table=11
                     /translation="MKKVNVTYAILYDKTNEKILMVKNKGENGSYYTLPGGAVKLGETL
                     EEAVIREVKEETGLHINVKGICSISEAFFEERDHHAIFFNFLGEIIGGETYISRPKEIE
                     EITWMELHIAAPYLRIPEHLLDLLQKKETVPYFFNGTIVHQSS"
     misc_feature    complement(9515..9528)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.32"
                     /tool="antismash"
ORIGIN
        1 ctttttttat ccattcagca gcaactcttt gggataacga actcgatcca tagtctgttt
       61 gcatttttat atctgacaat ctttcaataa ctggttcagg tccaataatc catccaattc
      121 taagtcctgg gctaagtgtt ttagatagac taccaacata taatacatgt ccatgtttat
      181 ctattgattt taaaggagct ggagggggtt catcaatcca taactcacga taaatatcat
      241 cttcaataat aggtaactgt tccttttcac atatctttat tatttctttt cttcgttctt
      301 gagacattaa tatcccagtt gggttttgaa aactaggaat agaatataaa atgtttttct
      361 tttgactgta tttaatgcgt tttaataatt cgttaggtaa aataccgtga tgatccatag
      421 ctataccaga taaatttata tttgctgatt gaaatacatg aagtgaatat aagtaagaag
      481 gttgttcaag taaaacagtt gattctctat gcaaaagacc aattgatatt aattgcaatg
      541 cttgtaatgc accagagacg attaatatag aacccggtga tacatgtatt ccgaatgatt
      601 ttacgtagtt acttattgcc tcacgtaacg gtaaaaaacc tttttgttct tcgtatccga
      661 aataatctaa ttcgtcactt acgtttttca taatggactg catcgttttt agcgggaaaa
      721 tttgaggtgc aagttcacct ttactaagat ggataagtgt tttatttgct tctgcttcat
      781 ttatttctcg taccattaac ttgctaggtt tatgaatacc agcttttaca tattcactcc
      841 aatcaggcgg gggattcgtc gctaataatg accatgtatt atttgttaca actgttcctg
      901 ctccgatttt cccttgaatt aagccatctg ccataagttc atcgagcgca gttattacag
      961 tacttcgatt tacatgaaat aattttgcta actgcctttg actagggatc ttacttccga
     1021 tcggccattc tccgttttcg attttttctt tcatataatc tactatctgc cgatatttag
     1081 gcagtttttt attcgttgtc ataaaaaaac tccctcgttc aatttaaaaa tgccaagtgg
     1141 ttggttttta cgataaataa ctggttgaag acattgtatc aactattaaa tatgatgaaa
     1201 agaaataatt ggtaatttaa aaatggttgg tttattagaa agggagagaa aacatgcata
     1261 ataaaaaatg ggatttacgc attatatatg cacacgcgtt tacgattctt atatggggaa
     1321 ctgcttttcc ggcaattcgt atggggcttg aatcttatac acctgagcat cttactttac
     1381 tacgcttatt aatcgcttca tttatacttc tgttgttttc ttttatctac aagttacggc
     1441 taccggattt aaaggatatc ccagcaattt ttatgttcgg tgctttagga ttcacttttt
     1501 atcacattgc attaaactac ggcgaaaaaa cagtaaatgc tggttctgca agtttaattg
     1561 tttcggtcac acctatagta acggcaattc ttgcttctat atttttaaac gaaaaaatga
     1621 aattaaatgg ttggatcggt ggtgtaatta gttttatagg aattgctctc atatcattta
     1681 gtcaaggaga tgctattcaa ttgaatagtg ggggattatt tatattatta gcagcgattt
     1741 cagaaagcct atattttgtt tttcaaactt cttacttaaa aaagtacggc ttcttaccat
     1801 ttaccatata tacaatttta tctagtactg tatgtatgct tattttctta ccgggaatgt
     1861 atacagaaat actagcagct cctcttgaag tgaatgtgag tgttttatat ttaggcttat
     1921 ttccaacagt actcccatat atcgcattgg cctatatcat atctcatgct ggtgctgctg
     1981 aggcaacaag ttctctatat ttaacgccag taactgcatg ttttattgct tggttatggt
     2041 taggagaagt gccaacttta gtttcgataa ttggcggagg gattactata cttggtatcg
     2101 tgattgctca tataccagta ttaagaaaag aaaaacatag gaatttagca aataatcaat
     2161 ccgtataacg taattcaata ataattttat gatacttttt catatagttt ctttcttatg
     2221 ttgataaata taatattttg tatttcagtt aaatattgta tgttacattt tatgtagtaa
     2281 gcattttaaa aggagatgac aacataatga atggaaaaag aaatattttc acatgtattt
     2341 ctattatagg aatcggtcta gctagttttt ctaattctag tttcgcagca aatgtaacgg
     2401 acaattcagt acaaaattct attcccgtag ttaatcaaca agtagctgct gcaaaggaaa
     2461 tgaaaccatt tccccagcaa gttaattatg caggtgttat aaaacctact catgttacac
     2521 aggaaagttt aaatgcttct gtaagaagtt actacgataa ttggaaaaag aaatatttga
     2581 aaaatgattt atcttcttta cctggtggtt attacgtaaa aggagagatt acgggtgatg
     2641 cggatgggtt taagccactt ggaacttcag aaggtcaagg gtatgggatg ataattacag
     2701 tattaatggc tggttacgat tcaaatgccc aaaaaatcta tgatggttta tttaaaacag
     2761 caagaacttt taaaagctct caaaatccta atttaatggg atgggttgtc gcagatagta
     2821 aaaaagcaca aggtcatttt gattctgcta ctgatgggga tttagatatt gcgtattctc
     2881 ttcttcttgc tcataagcag tggggatcaa atggaacagt taattattta aaagaagcac
     2941 aagacatgat tacaaaaggt attaaagcta gtaatgttac aaataatagc cgactaaatt
     3001 taggagattg ggattctaaa aattcacttg atacgaggcc atctgattgg atgatgtcac
     3061 accttagagc attttatgaa tttacaggtg ataaaacttg gcttactgtt attaataatt
     3121 tgtacgatgt ttatacgcaa tttagtaata agtactctcc aaatacaggg cttatttcag
     3181 atttcgttgt aaaaaaccca ccacaacccg cacctaaaga cttcttagat gagtcaaaat
     3241 atacaaatgc atattattat aatgctagtc gagtaccttt aagaattgta atggactatg
     3301 cgatgtacgg tgagaagcga agtaaagtca tttctgataa agtctcttcg tggattcaaa
     3361 ataaaacgaa tggaaatcct tctaaaattg tggatggtta tcaattaaac gggtctaata
     3421 ttggtagtta tccaactggt gtattcgttt cgccatttat tgctgcaagt ataacggata
     3481 gcaataatca aaagtgggta aatagcggtt gggattggat gaagaataag agagaaagct
     3541 actttagtga tagttataat ttattaacta tgttatttat tacaggaaat tggtggaaac
     3601 ctgtacctga tgataagaaa atagaaaacc taataaatga tgaaactcaa aaaggatacg
     3661 ataaataaca ggattttctt tttagtaaaa ggaattagca ctatgctagt tcctttttaa
     3721 tatttcgtga agcaatacag ctttgcactt aaataaagaa aaatttaata agatcatgtt
     3781 gtaggttgtt gtcggaaaag ggagaatatg tcactctgtt gaatataaaa ataccaccta
     3841 attataaagg agtctaaaat attgaatatc cagttaaaga ttgttacgag agcaaattgg
     3901 gaagatgcat tcaagttaca agttaaaaaa gatcaaagaa aatttgttcc atccgtagca
     3961 gtttcacttg ctaaagtata tataaaacca gatggtgacc atgtagaata cataccattt
     4021 gcgatatacg acggcgagct aatggtcggt tttgttatgc atgctgttat aaaagagaca
     4081 acagatatgt attggataaa tggatttatt atcgatcaaa agcagcaagg gaatggatac
     4141 ggaaaagcgg cattacagga aagtgtctat ttaataaaaa atacatataa ggcgtgtaaa
     4201 gaaattagat taacagtaca caaagataat atttctgcaa ggaaactata tgaatgttat
     4261 ggttttcaat cattgggaca tgattatgat ggcgagcaag tgtatcgttt atttgttaat
     4321 caaatataaa gtaaatgact gggaggaata gcgtttgaaa actaaatttc atcatattgt
     4381 acgagctgtt atgattaagg ataaaaaatt gttagtggct gagtatattg gccatcatta
     4441 ttttttacct ggtggccatg ttgaaattgg agaatcagca gagaatgcat taataagaga
     4501 gttaagagaa gaacttggag taaactgtag tatacaacaa tttttaggag tcatagaaaa
     4561 tcaatggcaa gataaagaag tgcttcatca tgaaattaac cacatttttg aagtagagtc
     4621 acaagattta catacggact taccaccgaa atctagcgag tctcatttag catttcaatg
     4681 gatagattgt aataaagaag ccttaaatca ttatgaaatc atgccaatgc ctttagtgaa
     4741 ggaattacta gaaagaaaat taagcgatga actactaaac tgttggatta gtaattttta
     4801 aatacctcta ttaatagtaa atacgtccac cgaaaggaaa gtaagaaaat aggctaaaaa
     4861 acaaggtaca tcacctagtg taagttcata ttttgttgat gtacgcattt agtctatagt
     4921 cttgtttcta ccttgtttgc aaaaatatac gaactttaac attgggacgg aatttatttt
     4981 tataaatgga gggtttcata atgaatcagt ttcaacaaga actacaatca ttaaatctta
     5041 atgattatca atctggtaat gttgtgtatt gggatcaact acaacaaaat caatatccat
     5101 actattacat ccaagacgat gcacgtcgtt gtggcggatg cggaggttgt ggtggtcgct
     5161 gtggtggatg tggcggtgga agatgcggag gttgtggtgg tcgttgtgta ggatgcgctg
     5221 gttgtttcgg ttgttttaac tgctggaact ggtggatcat ataagtatag aaaatcaata
     5281 atatttcgca tgaaagaaag gtgtaaatat tcttttatac ttttctttca tgcggtaatt
     5341 ttttattcgt gttaaggcgg atgtatttgt atacatatga taagttaatt tcttgggtag
     5401 aaaatataaa agaagaaaat catagttctg cagcagcact gagcattata aaagataata
     5461 aaattgtact agagcattat agtggttacc actcaaatac ctctacaagt aaaaaagtaa
     5521 ccgcgtcatc acaattcaac gttgcttctg cgagaaaaag ttatttaggt ttaatggtag
     5581 catacgcact ttacgaaggg aaaataaact ctattgatga tgcagcgata aagtatgtta
     5641 aagactttga ttctgcattg ctcggtaaaa caacgataag gcatttagta actcattcgc
     5701 acggtttagg agaaacgaat gatgggacaa tttttcgtga atttgaaccg ggacaagctt
     5761 gggcgtatag agatattaat gtaagaatga tgacacatct gatttatcag ctatataaca
     5821 aaagttttcc tgaattgtta aaggagcgtg tatttgatct agctaacttc aaagaaacag
     5881 gttggagaat tcagcaagat gaaaatttgg ttgaaattat taataatcca aatgaagacc
     5941 cgataagtgg aattggtaca gtaaatgatg gtactgaaaa aatctatttg tctcagctag
     6001 agaatttgcg cagtggggca atcttcatct aaatcaaggc atgatacatg ataaacaaat
     6061 cattccaaaa gaagttataa aaattgctac gagtttgcag agtccaacat ttataaacaa
     6121 agagctacca caaaatgggg tgttttggtt catccaaaat gagcctgcac aattaagcga
     6181 acttggtgaa cgtattccaa aagggtcgta tcaaatatta gggattactg gaccgactat
     6241 tttagtaata cctgaatata atgtagttgt tgcaaaaatg tataataaaa gatataacta
     6301 cggcggtgat aattatttat attatttacg tgaatttagc aatttagtcg ctgatacatt
     6361 tcgtaacggt aatagggcat aattactcct ataatgaaaa ggagtgagag aaagtgaaag
     6421 ataaaatgaa taaaaacaaa aagcaaaaga tcaaagatgt aaatacagaa caaaatgcga
     6481 tttatagcga tccgaaagat gcagctaata tgcaaacggt accgcagccg aaagattttg
     6541 atgaaattga atattaattt ttcgtccatc cgactatatg atgaatgaaa aatacatctt
     6601 aagtcccatc acatgattga aatcttttgg tattctattt acataagcat ttgaaaatgt
     6661 cttatcttaa caaagctttc tttaagtaaa tctaatataa cctgcaaaaa gacacttcat
     6721 tatggagtgt ctttttgtgt acatgaattc aatttccatt ttccgtacag tatcttactt
     6781 tttatgatgg atatactaac cgtgaaaatt catgtcaaga taaaagaagg ggacaagtta
     6841 gtatgaaaca aagttctata aatccgttac taattgttct aggtacaatc attgttcaaa
     6901 ttggccttgg aacaatttat acatggagtt tatttaatca accccttgta agtaagtttg
     6961 gatggaatct taattcagta gcgataactt tctccataac aagtttttct ttatcattct
     7021 caactttatt tgcagcaaag ttgcagaaaa aattaggact tcgaaaactt attgctactg
     7081 caggaattgt tttaggactc ggcttaatac ttagttcaca agtttcttct ttaccactat
     7141 tatatttatt agctggtgtc gttgttggtt atgcggatgg aacagcttat attacatcac
     7201 tatctaattt aattaaatgg tttccaaatc ggaaagggct tatttcaggt atatctgtat
     7261 cagcgtatgg aatgggcagc ttaatcttta gatatataaa cggaaatctt atcaataacc
     7321 ttggtgtatc acaagcattc ttatattggg gtattatcgt gttactttta gtgttaatcg
     7381 gatcgttctt cttacgtgag gcaattgtaa gtaatgctgt aactgaaaca ttacacaatg
     7441 actatactcc gcgtgaaatg atgcgaacga aacaagtata tctcctattt tttatgttat
     7501 tcacctcgtg tatgggtggt ttgtatttaa taagtatggt aaaagatatc ggcgtacaac
     7561 tcgttggact tagcgcagca accgctgcaa acgccgttgc tatgattgca atctttaata
     7621 cagtaggtag aattattctt gggacgctat cagataaaat cggccgaatg aaaattgtct
     7681 ctgcaacgtt tattattata ggattgtcag tctttacttt aagttttatt ccgctaaatt
     7741 acggaatcta ttttgcttgt gtagcaagtg tcgccttttg cttcggtggt aatataacta
     7801 tattcccagc tattgtcgga gatttctttg gattaaaaaa ccatagtaca aattacggca
     7861 ttgtctacca aggtttcgga tttggtgcgc ttgcaggatc atttattgga gcgatactcg
     7921 ggggatttca accaactttc attataatcg gtgttttaag tgttatttcc ttcattattt
     7981 caatattaat ccgtccacca actgcagaaa agaaaaagga actaaaacat ttacatcgga
     8041 aagttgctta attttaaaaa gagtcctcat gttagggctc ttttttattc cattaacttt
     8101 gcttctttac taactcatta aagacaggac aatatcatta taaaattgaa tagataagta
     8161 aaaaggagcg tgacacttaa aatgaaaata gtacagcagt ggatacaaga agatagtgat
     8221 tacataagag agaaagtaat tgaatataat caaaaatatc tcgcagatga agaaaaaaca
     8281 ccttcagaaa aggttagttt catagtaaga aatgaaaaag aagaaatcgt aggtggggta
     8341 acagcaataa ttttttggca ccatgtacac gttgactttc tatgggtttc tgaagaatat
     8401 aggcatgaag gttacggaaa caagttaata aagcttattg aagaatttgc aattgaaaaa
     8461 gggtgtaggt taattaattt agataccttt agtttccaag ctcctgattt ttataagaaa
     8521 catggatata aagtaattgg agtaagtgaa gatcatccga aaggacataa tcattattat
     8581 ttagaaaaac ggctaaaaag tatatagaat ttgttaacat tagtatttgg aatacaaatg
     8641 taatcaaggc gaggttatta ttaattttag ttttaattta atcaattttc atgctaaaac
     8701 ttcaagaacg ttctttggat aatcttccta agatacgttc tttctttata tatcagtggc
     8761 ccattcttat taactccacc aatagcggta aaggcaagac ttaactaact tatatatatt
     8821 aaaaggagtt aatggtagaa tggatagaag acagaaaatt ccttttatta aaaatagagg
     8881 aggtacatat gaaaaaagtt aatgttacat acgcgatttt gtacgataaa acaaacgaaa
     8941 agatcttaat ggtaaaaaac aaaggagaaa atggttctta ttatacatta ccaggtggcg
     9001 cagttaaatt aggagaaaca ttggaagaag cagtaattcg agaagtaaaa gaagaaacag
     9061 ggttacatat aaacgtaaaa gggatttgtt ctatcagcga agcctttttc gaggaaagag
     9121 atcatcatgc aattttcttt aattttctag gcgaaataat cggcggggag acatatatat
     9181 caagaccaaa agaaatcgaa gagattactt ggatggaatt acatatagca gcaccgtatt
     9241 tacgcatacc agaacattta ctagatttat tacaaaagaa agaaacagta ccttattttt
     9301 ttaacggaac aatcgttcat caatcttcat aaaaatatta tctcatgtaa ggcggggaaa
     9361 acatggagtt catcgttaat cacaaacaat ttactcaagc actttcagaa gttaacaaag
     9421 cgatatcaac aaaatcttta attcctatat tatctggtat aaaaataact gcagatcaat
     9481 ctggaattac tttaattgca agtaactcga atatttttat tgaaaaattc attcctgttt
     9541 caattgaaga cgaaaaaatt gcaactattt taaaggcagg aactattgtt gtacctgcaa
     9601 agtatttcat tgaaataata aagaaaatgc caagtgatat agcaataaaa agtaagaatg
     9661 agcaaattat taagatacaa tcagaagaaa tcacattaag tttaaatgga tttcctgcag
     9721 atgagtttcc gaatgtaccg cttatagaca atcatgcaga aatcaaggta gagacagagc
     9781 aattgattga agtatttaaa caaacagtct ttgcggtagc gaaaaatgaa tctagacctg
     9841 ttcttactgg ggtgcatata gagttatcta ataataagtt tatttgtgct gcaactgact
     9901 cacatagact agcgatacgt gaaacgcgac tttcctcaga tgtaaaagca aattgtatcg
     9961 taccaagcgc aaccattagt gaacttctaa aattaatgaa cagcaattcg gaattcgtat
    10021 atatttacct ttcagagagt catattatat ttacgcttgg aacaactaca ttatattcaa
    10081 gactaattga agggaaatat cctaatattt ctaaccttat tccaaatgac tttaaaacga
    10141 ttattaacat agatagaaaa aagatattac aaggtgtaga tcgatcaagt ttattagcaa
    10201 gtgaatgggc caataacaat gttaacttag aaatcataaa cgaatctaca attaaaattt
    10261 cttc
//
