LOCUS       NZ_MOOT01000033         2615 bp    DNA     linear   CON 20-MAY-2024
DEFINITION  Bacillus thuringiensis serovar dakota strain BGSC 4R1 contig000033,
            whole genome shotgun sequence.
ACCESSION   NZ_MOOT01000033
VERSION     NZ_MOOT01000033.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis serovar dakota
  ORGANISM  Bacillus thuringiensis serovar dakota
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Comparative genomics of Bacillus thuringiensis reveals a path to
            pathogens against multiple invertebrate hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, 1st Shizishan,
            Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.5.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002148095.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 01:21:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,175
            CDSs (total)                      :: 6,055
            Genes (coding)                    :: 5,836
            CDSs (with protein)               :: 5,836
            Genes (RNA)                       :: 120
            rRNAs                             :: 4, 7, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 7, 6 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 88 of 219
            Pseudo Genes (incomplete)         :: 138 of 219
            Pseudo Genes (internal stop)      :: 66 of 219
            Pseudo Genes (multiple problems)  :: 56 of 219
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:21:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2615
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOOT01000033.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..2615
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:2567](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..2567)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..2567)
                     /locus_tag="BK765_RS06600"
                     /old_locus_tag="BK765_06305"
     CDS             complement(<1..2567)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (157-225). E-value: 7e-22. Score:
                     69.4. Matches aSDomain: nrpspksdomains_BK765_RS06600_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (249-548). E-value:
                     9.5e-107. Score: 348.4. Matches aSDomain:
                     nrpspksdomains_BK765_RS06600_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="BK765_RS06600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_06305"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_256942075.1"
                     /sec_met_domain="PP-binding (E-value: 2.6e-18, bitscore:
                     52.6, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.1e-73, bitscore:
                     234.0, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 3.6e-31, bitscore:
                     94.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQPELTKASFIPHPFKNSSNDRLYRTGDLVKYLPDGNIEYIGRA
                     DKQVKIRGFRIELGEIETILGNHPDIKEVTVVAQEDSFGDNILVAYIVGEGDTQEWRKH
                     VGVHLPNYMVPAHFIKIESLPLTVNGKVDKDALPAWASIIQTNEGYIAPRNRVEKKMVE
                     IWSEVLGIDSSAIGINDNFFELGGHSLLATKITSRLGVDFSILVQIRDIFEYPTIKHFS
                     KRLTFLLGKTGEIDAFTPLQSVKREQYEPLSFAQQRLWIMDKIVTNSALYNVPIAWRLK
                     GKWNIEMLKKGYNAIIHRHEILRTVFHEVDGKPVQIVQQDITVPLSVIDLRYLSPEDKT
                     SKIENISKIEAKKPFDLSQGPLLRTHLIIMDNKELVLLCTIHHIIFDRWSLDTFINEWM
                     SFYQAFLENETEKLPPLPVQYIDFTKWQKSWLTEDIIQKQIAYWGKELRGLLPILELPL
                     DYPRPIIQSYNGDTYQIVIPNKLLEKIKVFNSKEGVTLFATLLASYQGFLSRYTNQKDI
                     LVGSPIANRNYPGVEDLIGFFANTVVYRADCSNNITFKELVQQIKKKMLDAQENQDVPF
                     EKVIEAIQPERDMSYSPLFQTMFVFHNQLKELPKLLDHSVEKIPRHMNGAKFDITIAME
                     EIPTGLQVDFEYNTDLFKVSTIKRMAQSFEIWLDEILQFPEEPIENLRILTKNEEKQLL
                     HEWTNIEGNYQKDVVIQELFEQQVIQNPDSVALVYKDQQLTYKELNEKVNQLAFYLQKR
                     NIGPESMVGVYIERSLEMIVSILGIIKAGGAYVPLDPAYPTKRLEYILKDANIQVLLTQ
                     SHLTQWIPKEIDCIDIKEHEMNISREKNINPTIEVKPDNLAYV"
     source          1..2615
                     /db_xref="taxon:132268"
                     /mol_type="genomic DNA"
                     /organism="Bacillus thuringiensis serovar dakota"
                     /serotype="H15"
                     /serovar="dakota"
                     /strain="BGSC 4R1"
                     /submitter_seqid="contig000033"
     region          1..2615
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2615
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(201..242)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK765_RS06600_0007"
                     /evalue="9.60E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="BK765_RS06600"
                     /protein_end="789"
                     /protein_start="775"
                     /score="18.7"
                     /tool="antismash"
                     /translation="LGIIKAGGAYVPLD"
     CDS_motif       complement(765..881)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK765_RS06600_0006"
                     /evalue="2.10E-22"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="BK765_RS06600"
                     /protein_end="601"
                     /protein_start="562"
                     /score="71.6"
                     /tool="antismash"
                     /translation="KKMLDAQENQDVPFEKVIEAIQPERDMSYSPLFQTMFVF"
     aSDomain        complement(924..1820)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK765_RS06600_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="9.50E-107"
                     /label="BK765_RS06600_Condensation_LCL.1"
                     /locus_tag="BK765_RS06600"
                     /protein_end="548"
                     /protein_start="249"
                     /score="348.4"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIFDR"
                     /tool="antismash"
                     /translation="EPLSFAQQRLWIMDKIVTNSALYNVPIAWRLKGKWNIEMLKKGYN
                     AIIHRHEILRTVFHEVDGKPVQIVQQDITVPLSVIDLRYLSPEDKTSKIENISKIEAKK
                     PFDLSQGPLLRTHLIIMDNKELVLLCTIHHIIFDRWSLDTFINEWMSFYQAFLENETEK
                     LPPLPVQYIDFTKWQKSWLTEDIIQKQIAYWGKELRGLLPILELPLDYPRPIIQSYNGD
                     TYQIVIPNKLLEKIKVFNSKEGVTLFATLLASYQGFLSRYTNQKDILVGSPIANRNYPG
                     VEDLIGFFANTVVYRADC"
     CDS_motif       complement(933..1022)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK765_RS06600_0005"
                     /evalue="1.30E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="BK765_RS06600"
                     /protein_end="545"
                     /protein_start="515"
                     /score="49.7"
                     /tool="antismash"
                     /translation="DILVGSPIANRNYPGVEDLIGFFANTVVYR"
     CDS_motif       complement(1275..1313)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK765_RS06600_0004"
                     /evalue="2.20E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="BK765_RS06600"
                     /protein_end="431"
                     /protein_start="418"
                     /score="13.9"
                     /tool="antismash"
                     /translation="QYIDFTKWQKSWL"
     CDS_motif       complement(1362..1427)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK765_RS06600_0003"
                     /evalue="3.10E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="BK765_RS06600"
                     /protein_end="402"
                     /protein_start="380"
                     /score="26.2"
                     /tool="antismash"
                     /translation="IHHIIFDRWSLDTFINEWMSFY"
     CDS_motif       complement(1644..1760)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK765_RS06600_0002"
                     /evalue="2.70E-14"
                     /label="C2_LCL_024-062"
                     /locus_tag="BK765_RS06600"
                     /protein_end="308"
                     /protein_start="269"
                     /score="45.5"
                     /tool="antismash"
                     /translation="ALYNVPIAWRLKGKWNIEMLKKGYNAIIHRHEILRTVFH"
     aSDomain        complement(1893..2096)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK765_RS06600_PCP.1"
                     /evalue="7.00E-22"
                     /label="BK765_RS06600_PCP.1"
                     /locus_tag="BK765_RS06600"
                     /protein_end="225"
                     /protein_start="157"
                     /score="69.4"
                     /tool="antismash"
                     /translation="EKKMVEIWSEVLGIDSSAIGINDNFFELGGHSLLATKITSRLGVD
                     FSILVQIRDIFEYPTIKHFSKRL"
     CDS_motif       complement(2382..2447)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK765_RS06600_0001"
                     /evalue="1.70E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="BK765_RS06600"
                     /protein_end="62"
                     /protein_start="40"
                     /score="39.2"
                     /tool="antismash"
                     /translation="YIGRADKQVKIRGFRIELGEIE"
ORIGIN
        1 attacatatg ccaaattatc tggtttaact tcaattgtag gatttatatt tttttctctc
       61 gaaatattca tttcatgttc ttttatatca atacagtcta tttctttagg tatccactgt
      121 gttagatgac tttgtgtcag caatacttgt atgttggcat ctttcaagat atattcaagc
      181 ctctttgttg gatatgcggg atctagtggg acatatgcgc caccagcttt tattattcct
      241 aatatactta caatcatctc taaagaacgt tctatataaa cccctaccat actttcaggg
      301 cctatgtttc gtttttgtag ataaaaagct aattgattta ctttttcatt taattcctta
      361 taagttaact gttgatcttt atataccaac gcaacagaat ctggattctg aattacttgc
      421 tgttcaaata actcttgaat caccacatct ttttgataat tgccctctat gttagtccat
      481 tcatgtaata attgcttttc ttcgttctta gtgagtattc ttagattctc aataggttcc
      541 tccggaaatt gtaaaatttc atctagccaa atttcaaaag attgagccat tctttttatt
      601 gtagaaacct taaataagtc tgtattatat tcaaaatcca cttgtaatcc tgttggtatc
      661 tcttccattg ctatagttat atcaaacttt gcaccattca tatgtctagg gattttttct
      721 acgctatgat ctaataattt tggtaactct tttaattgat tgtgaaaaac aaacattgtc
      781 tgaaataatg gagaataact catatcacgt tctggttgta tagcttctat aactttttca
      841 aaaggaacgt cctggttttc ttgtgcatct aacatttttt tctttatttg ttggaccaat
      901 tctttaaatg ttatattatt tgagcaatct gcacgataga ctacagtatt tgcaaagaat
      961 ccaataagat cttcaactcc tggataattc ctatttgcaa tcgggctgcc tactaaaata
     1021 tctttttgat tggtataacg agaaagaaac ccttgataac ttgctaataa tgtggcgaat
     1081 aatgtaacgc cttccttaga attgaatact tttatttttt ctaacaattt attgggtatg
     1141 acaatttgat acgtatctcc attatagctt tgtattattg gacgaggata atccaatggg
     1201 agttccaata taggaagtaa accgcgtaat tctttccccc aatatgctat ttgtttttgt
     1261 ataatgtctt ctgttaacca acttttttgc cattttgtaa aatcaatata ctgaacaggt
     1321 aaagggggaa gtttttcagt ttcgttttct aaaaatgcct gataaaaact catccattca
     1381 ttgataaagg tatctaatga ccagcgatca aaaataatat gatggatggt acaaagaagt
     1441 actaactctt tattgtccat tattattaaa tgagtacgta ataaaggtcc ttggcttaaa
     1501 tcaaatggct ttttagcttc tatttttgat atattttcta ttttagatgt tttatcttca
     1561 ggagaaagat aacgcaaatc aattactgat aagggaactg ttatatcttg ttgaacaatt
     1621 tgcactggtt ttccgtcaac ttcatgaaat actgtccgta gaatttcatg gcgatgtatt
     1681 attgcattat atcctttttt taacatctca atgttccatt ttccttttaa acgccatgca
     1741 attgggacat tgtaaagcgc actatttgtt acaattttat ccattatcca taaacgctgt
     1801 tgagcgaaag ataatggttc gtattgttca cgtttaactg attgtaatgg tgtaaaagca
     1861 tcgatctccc ccgtttttcc caataaaaat gttaaacgtt ttgagaaatg tttaatggta
     1921 gggtattcaa atatatcacg tatttgtact agaattgaaa aatcaacacc taaacgtgaa
     1981 gttattttag ttgctaggag agagtgtcca cctaactcaa aaaaattatc gtttattcca
     2041 atggcagagg agtcaatccc taatacctct gaccaaattt ctaccatttt tttctctact
     2101 ctattccgag gtgctatata tccctcgttc gtttgtataa tagaggccca tgcaggtaaa
     2161 gcatctttat ctaccttccc attaacagtt aatggcaatg attctatctt gataaaatga
     2221 gccgggacca tataatttgg aagatgtacc cctacatgtt ttctccactc ttgtgtatct
     2281 ccttcgccca caatataggc tactaagatg ttatctccaa atgaatcttc ttgcgctacc
     2341 acagtaactt ctttaatgtc tggatgattt cctaatattg tttcaatttc acctaactca
     2401 attcgaaatc ctctaatctt aacttgttta tcagctcttc ctatatattc tatatttcca
     2461 tcaggtaaat atttaactaa atcaccagtc cgatataatc tatcattaga gctattcttg
     2521 aatgggtgtg ggataaatga ggcctttgtt aattcaggtt gattcaaata tcctcgtgct
     2581 aatgcgatac ctcctatata aagttcacct actgt
//
