LOCUS       NZ_MOOT01000010        17689 bp    DNA     linear   CON 20-MAY-2024
DEFINITION  Bacillus thuringiensis serovar dakota strain BGSC 4R1 contig000010,
            whole genome shotgun sequence.
ACCESSION   NZ_MOOT01000010
VERSION     NZ_MOOT01000010.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis serovar dakota
  ORGANISM  Bacillus thuringiensis serovar dakota
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 17689)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Comparative genomics of Bacillus thuringiensis reveals a path to
            pathogens against multiple invertebrate hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17689)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, 1st Shizishan,
            Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.5.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002148095.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 01:21:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,175
            CDSs (total)                      :: 6,055
            Genes (coding)                    :: 5,836
            CDSs (with protein)               :: 5,836
            Genes (RNA)                       :: 120
            rRNAs                             :: 4, 7, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 7, 6 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 88 of 219
            Pseudo Genes (incomplete)         :: 138 of 219
            Pseudo Genes (internal stop)      :: 66 of 219
            Pseudo Genes (multiple problems)  :: 56 of 219
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:21:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 17689
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOOT01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..17689
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[15694:>17689](-)"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(15695..>17689)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     gene            <1..114
                     /locus_tag="BK765_RS31770"
                     /pseudo=""
     CDS             <1..114
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418478.1"
                     /locus_tag="BK765_RS31770"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="IIKLGNNISNTEKIFGLGILLYILETIKVRKSRIFND"
     source          1..17689
                     /db_xref="taxon:132268"
                     /mol_type="genomic DNA"
                     /organism="Bacillus thuringiensis serovar dakota"
                     /serotype="H15"
                     /serovar="dakota"
                     /strain="BGSC 4R1"
                     /submitter_seqid="contig000010"
     region          1..17689
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /product="RRE-containing"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX) and not alpha_am_amid)"
                     /rules="minimum(1, [Bottromycin_Methyltransferase_RRE,
                     Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /tool="antismash"
     cand_cluster    1..17689
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /detection_rules="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /kind="neighbouring"
                     /product="NRPS-like"
                     /product="RRE-containing"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    1..17689
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[9237:10341](-)"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      complement(9238..10341)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="2"
     gene            complement(296..676)
                     /locus_tag="BK765_RS01780"
                     /old_locus_tag="BK765_01695"
     CDS             complement(296..676)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017149753.1"
                     /locus_tag="BK765_RS01780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01695"
                     /product="hypothetical protein"
                     /protein_id="WP_000771854.1"
                     /transl_table=11
                     /translation="MKLLRSFLIFTLVVLLSACNTATKVTKVQKNGETTLTIGSLQGYY
                     DVQTLKAEKGNVEIPYEAAVEEGTILLQVTKDDKVIYEEEVTSQKEGLLSFEAPKSGSY
                     DLIVRVKGEEEKAKGIQIHTKL"
     gene            complement(757..1041)
                     /locus_tag="BK765_RS01785"
                     /old_locus_tag="BK765_01700"
     CDS             complement(757..1041)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531359.1"
                     /locus_tag="BK765_RS01785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01700"
                     /product="DUF4176 domain-containing protein"
                     /protein_id="WP_000250499.1"
                     /transl_table=11
                     /translation="MVQNLELELLPIGSVVMFKDWEHPLMVYGRKQMDSETKRTWDYVC
                     CYFPHGNISSEYNFFLNHEDISSVLHLGFINETELEFQKLFKKEVEEKR"
     gene            complement(1043..1252)
                     /locus_tag="BK765_RS01790"
                     /old_locus_tag="BK765_01705"
     CDS             complement(1043..1252)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036918.1"
                     /locus_tag="BK765_RS01790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01705"
                     /product="hypothetical protein"
                     /protein_id="WP_001036918.1"
                     /transl_table=11
                     /translation="MNKKVKNFKYLMIILACIAIFGTILPNALDPNETLAGNISVAVFG
                     TIGTCLLFSVTYFIIKKAILRRGN"
     gene            complement(1267..2523)
                     /locus_tag="BK765_RS01795"
                     /old_locus_tag="BK765_01710"
     CDS             complement(1267..2523)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056068.1"
                     /locus_tag="BK765_RS01795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01710"
                     /product="T7SS effector LXG polymorphic toxin"
                     /protein_id="WP_086397613.1"
                     /transl_table=11
                     /translation="MSLNMYLGEVQNQTQSMNAVCTATIQGMEQAIQSIDAFAVDTVLQ
                     GQTYSSAKAFFVQTFRPLAQGIIYLCEELIRQNDAFPSQFQSQVASTDVIEQEILEQIR
                     EIDRMKTSMEAVNQAMPMPGMDAMVNLFIVMRKKLQEKLDHLYQFNQTSSNNYSTAIQL
                     AASIAAGLAEVQSGKGFNALTGIFSVKELNMEWTVAIQKKSEDKVHAVQIEKEAVKNDW
                     FLMDSKEVKETANGVATVTGVVVKGIGSIINGKKYEKSFTEEKGYGGIFIKGLTLEAEP
                     FVSSRTYDQFNVKDKEKREQTYGPKVGFTVAQAGIEQEDIVFGAGAEAKVALASAELGA
                     HVENGNLHIGAEATLVKYESAIKIPIPFMDKKLQIGGSASLGTLGGSFEAGKSGFKFHA
                     PFGPGVGLWGFGLEVSVKE"
     gene            complement(2520..2882)
                     /locus_tag="BK765_RS01800"
                     /old_locus_tag="BK765_01715"
     CDS             complement(2520..2882)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016121984.1"
                     /locus_tag="BK765_RS01800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01715"
                     /product="DUF3958 family protein"
                     /protein_id="WP_086397614.1"
                     /transl_table=11
                     /translation="MSQDIEKQINQVNQKLRSVFEEQDRNQSAIQAQEQVEADFYECRS
                     RNRRLFERILGTWHGDREMSQFFMNTYQDAQHIERKVTFELENKKETLFKEKRDLSDLE
                     NALSYQQQQLVKEANS"
     gene            complement(2923..3198)
                     /locus_tag="BK765_RS01805"
                     /old_locus_tag="BK765_01720"
     CDS             complement(2923..3198)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529644.1"
                     /locus_tag="BK765_RS01805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01720"
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="WP_000529663.1"
                     /transl_table=11
                     /translation="MGQFQSNLQTATQIATKMGSAPDRIQSATTRSITKATRTTLSVNF
                     KAQEANQQMLDLTKQFSAAFQQAVDNIHSVSNEFERMDNELHNTFR"
     gene            complement(3361..3657)
                     /locus_tag="BK765_RS01810"
                     /old_locus_tag="BK765_01725"
     CDS             complement(3361..3657)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016098426.1"
                     /locus_tag="BK765_RS01810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01725"
                     /product="WXG100 family type VII secretion target"
                     /protein_id="WP_000918588.1"
                     /transl_table=11
                     /translation="MAGQIRMSPEELKSKAARYGQGANQIEDILSQLQNLQNELRGEWE
                     GRAFEGFDQQFNELKPKVQNFAQLLQEINMQLNKTAEAVASHDEELSRNFGLK"
     gene            complement(3868..5229)
                     /locus_tag="BK765_RS01815"
                     /pseudo=""
     CDS             complement(3868..5229)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1164: transposase
                     IS3/IS911 family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611143.1"
                     /locus_tag="BK765_RS01815"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="IS3 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTKFTSEDKMNAVIHYQDGSESIKDIAKSLGANHEVVRMWIKQFE
                     YHGIRAFEKGYTTYSAQYKLDVLNYMFENGTSPNETAVIFNISSPGLIRKWRIQLHQKG
                     VDALKSKKKGRQSMTKDNQKSIKKQTQTEESVETLQAELERLRMENAYLKKLNALVQNK
                     EKSPNKIRRK"
     misc_feature    complement(5252..5265)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.94"
                     /tool="antismash"
     misc_feature    5253..5266
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.67"
                     /tool="antismash"
     gene            complement(5307..5636)
                     /locus_tag="BK765_RS01820"
                     /old_locus_tag="BK765_01740"
     CDS             complement(5307..5636)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566267.1"
                     /locus_tag="BK765_RS01820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01740"
                     /product="hypothetical protein"
                     /protein_id="WP_000566275.1"
                     /transl_table=11
                     /translation="MIDLLNKWMLESTGNFNIVVGITALLFLGSVIALIIIYKKIGKPD
                     ESTNAIYLKITSRMFTTQILMNAIFISLVGKDIENFRQIFILFEAFVFFIGAIYSFKLY
                     RQEYK"
     gene            6374..6523
                     /locus_tag="BK765_RS32260"
     CDS             6374..6523
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016097815.1"
                     /locus_tag="BK765_RS32260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000473828.1"
                     /transl_table=11
                     /translation="MFKKLVVGALATGISLIVSAFGTPFNNRMQVLAMLHSKFVEDSVL
                     VQKC"
     gene            complement(6563..>6896)
                     /locus_tag="BK765_RS01830"
                     /pseudo=""
     CDS             complement(6563..>6896)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259899.1"
                     /locus_tag="BK765_RS01830"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="DDE-type integrase/transposase/recombinase"
                     /pseudo=""
                     /transl_table=11
                     /translation="KVKGQWI"
     gene            complement(6978..7211)
                     /locus_tag="BK765_RS01835"
                     /old_locus_tag="BK765_01745"
     CDS             complement(6978..7211)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005923.1"
                     /locus_tag="BK765_RS01835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01745"
                     /product="hypothetical protein"
                     /protein_id="WP_001005923.1"
                     /transl_table=11
                     /translation="MNESVRKSEGELKKYEFTASELEMLFSGFYVAVRQLPENIGPKIY
                     EAIRCKSEDVLLKCLEESIRGSEEKKKEKSGF"
     misc_feature    7253..7266
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.4"
                     /tool="antismash"
     gene            complement(7492..7971)
                     /locus_tag="BK765_RS01840"
                     /old_locus_tag="BK765_01750"
     CDS             complement(7492..7971)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014471322.1"
                     /locus_tag="BK765_RS01840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01750"
                     /product="DinB family protein"
                     /protein_id="WP_001033113.1"
                     /transl_table=11
                     /translation="MNKIELIIMNSNEVRRRSLNLWSSIPSEVLFHRVDSSSMTILEQV
                     RHVLEAEYLYLEILKTRKSLPYSDDEPNPFNTRDFTTIQDEVDFAKPYRKNFLDFIMGL
                     NEEDLENIKIDRSDVGYTRNLGDMLMRMAYHEAVHTGQLLRDMRALGLKRPHLWD"
     gene            complement(7999..9231)
                     /locus_tag="BK765_RS01845"
                     /old_locus_tag="BK765_01755"
     CDS             complement(7999..9231)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937089.1"
                     /locus_tag="BK765_RS01845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01755"
                     /product="MFS transporter"
                     /protein_id="WP_000937089.1"
                     /transl_table=11
                     /translation="MLPKVFLNKNYVILFSGMTISKLGMWFFTIAIPLIVYDLTHSPSK
                     MAFVLALELGAQVIFSLIGGALADQMSKKKIMIIGDYASALFVLVVPILFLSGYNSIIV
                     IYIAAFTLSALAAFHHPSFESAVPEILSKEDLIQGNSFFRLSETIITFLGPAVAGIAIA
                     LFGNSNVLLITSVAFFLSGTIFLFLKFRDFAEEEKKRPELVKPIVEGLRYVFGTKIILV
                     GSLVIFGINIGFGAVESLFMYYLKDYLGLSPINIGLIFSLQAIGPILAVYFASKFKHIP
                     RGNVIIYSGIVIGLAQMLLYFSQFSLVILVFCQIFIKGSVTLLAINWFTLRQETVPSKL
                     LGRVISSTRMVAFLALPFSAMIAGFLAESINVLSIFLVAGAFAIVSSIVGVSLGLFDRK
                     RLDKIQNDTAL"
     gene            complement(9238..10341)
                     /locus_tag="BK765_RS01850"
                     /old_locus_tag="BK765_01760"
     CDS             complement(9238..10341)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Pantocin_Microcin_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ThiF"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802791.1"
                     /locus_tag="BK765_RS01850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01760"
                     /product="ThiF family adenylyltransferase"
                     /protein_id="WP_000802788.1"
                     /sec_met_domain="Pantocin_Microcin_RRE (E-value: 7.2e-30,
                     bitscore: 93.6, seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="ThiF (E-value: 1.1e-46, bitscore: 149.6,
                     seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPKFKETIPYFFLNKEIQIGEEEGLAGIIPDPTGSISFLINKLD
                     GQRNIPNIVQEVQKEFPDISSNEIIEAIKVLGNEGYLEDNSIVPELNDYILERYKANIN
                     YFSLFTKFGEDKYKIQEKILETPIALLGVGGLGTQVLYHLAALGFHNIKALDFDNIELS
                     NFNRQLLYSESDIGNSKVEMAKKRISQFNPNVDLQIINKKIESPQDVIEHIEGTELVIC
                     VADKPTLHIANWVNEGVVKCNLPMVYGGVLNTRGRFFSMIPSKTGCVQCHINYARQLND
                     KQDEQLEAMNNMEFTRNNAAISPNVAIVAGTIVNEALKILTQIAQPISLGKVMEVDFLT
                     LETNTVSSWEKMLDCPLCGQVVKSEVK"
     aSDomain        complement(10060..10341)
                     /aSDomain="Pantocin_Microcin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing E1-like protein in a
                     pantocin/microcin cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_BK765_RS01850_Pantocin_Microcin_RRE.1
                     "
                     /evalue="9.60E-30"
                     /identifier="RREFam017.1"
                     /label="BK765_RS01850"
                     /locus_tag="BK765_RS01850"
                     /protein_end="94"
                     /protein_start="0"
                     /score="93.6"
                     /tool="antismash"
                     /translation="MKPKFKETIPYFFLNKEIQIGEEEGLAGIIPDPTGSISFLINKLD
                     GQRNIPNIVQEVQKEFPDISSNEIIEAIKVLGNEGYLEDNSIVPELNDY"
     misc_feature    10577..10591
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.5"
                     /tool="antismash"
     gene            complement(10634..11434)
                     /locus_tag="BK765_RS01855"
                     /old_locus_tag="BK765_01765"
     CDS             complement(10634..11434)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016097814.1"
                     /locus_tag="BK765_RS01855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01765"
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="WP_000394562.1"
                     /sec_met_domain="PEP_mutase (E-value: 8.5e-65, bitscore:
                     209.0, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFKEKIKLFRTLHDQNNPLMLPNAWDASSAKIFERNDFQAIGTT
                     SAGIAATLGYPDGEKIPLELMLNSISNISKNTKLPVSADIEGGYAANIFDLRKILNELE
                     KFEVVGINIEDGRGTYLREINEQKVILDFIKAYSPHLFINARIDSFWLDIGDLKDRIKD
                     VIVRAEEYKKSGADCIFIPGVKDIETIKVLRENIELPINLLQSENLNSMQNIKTLKIER
                     VSLGSSAFRNILTNLDKISSTFKQGKLLHSNGNLTYQDLNLLMD"
     misc_feature    11475..11490
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 23.85"
                     /tool="antismash"
     gene            11557..13458
                     /locus_tag="BK765_RS01860"
                     /old_locus_tag="BK765_01770"
     CDS             11557..13458
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NpaA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684559.1"
                     /locus_tag="BK765_RS01860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01770"
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="WP_000684560.1"
                     /sec_met_domain="NpaA (E-value: 1e-188, bitscore: 618.9,
                     seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIAIVGLGPRGISVFERIVSNILSELKSESYEISLVDPHPPGGA
                     VWRTDQSPQLLMNTVASQITLFTDDSVQCRGPIVNGPSLLEWANELRDKIDTLDMPTVI
                     TEEIYSLEPNSYPTRALYGYYLKDSFNTILEELPSNIKINLIQDTLINLYPNNEDYTLF
                     LNKSLTPIKAEKVIFTTGHSDQKLTSAEEHFFEFANSNNLNYFPPSNPADVNLDSIEPK
                     TPVIIRGLGLNFFDYMSLLTSGRDGKFEEINDKLNYIPSGKEPLLIAGSRRGLPYHSRG
                     QNQKGSSERHIPLFLKNEVIEQLKHKNNLSFKKEIWPFVKLEVESIYYTTLIRNLYGIE
                     KSCQFLYEFVNCAIEYHKDLLINFGVKSELFFDWHKISCPYGDKKFNSITDFHNWLDTY
                     LHNDINEANLGNKNSPLKAALDVLRDIRNEIRILVDHGGINAESYKEELRNWYTPLNAF
                     LSIGPPVQRIKELRALIRAGILVLVGPEATVATSPINKDFKISSNRVKDSIFHAKNLID
                     ARLPSVNINQSKNILISNMLKAGLASEFKIATNNNYIFESGGLAVTPPPNRLIDKNGKV
                     NPNLYSFGIPTEGVHWVTAAGARPGVNSVTLLESDSIARDVLGCNITINNVYKQTIS"
     gene            complement(13494..14849)
                     /gene="purB"
                     /locus_tag="BK765_RS01865"
                     /old_locus_tag="BK765_01775"
     CDS             complement(13494..14849)
                     /EC_number="4.3.2.2"
                     /GO_function="GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA]"
                     /GO_process="GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="purB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089379.1"
                     /locus_tag="BK765_RS01865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01775"
                     /product="adenylosuccinate lyase"
                     /protein_id="WP_001089379.1"
                     /transl_table=11
                     /translation="MNSTPIDSEIFKDQFGTKQMRTNFSDENLIQLWLNSEVALAKAEA
                     KLGIIPQNAATNIANAADQNKLDFNKIREGMKKTGHPFVAFLHEFESLCDEESRKYIHW
                     GATTQDIIDTGLTLQIKGAHELIYNQTQEILKICLRLSQQYKSTVMPGRTLGQHALPIT
                     FGYKISIWSDELGRHLERLQQGADRYLVGQLGGAVGTLASLGAQGLAVRKEYVKNLNLI
                     EPLVSWHTSRDRFAEFSMILNMIGATTAKVAEEIISLQGTEIREVEEGYHSGKVGSSTM
                     PHKRNPMTCSYIVGLTKIVNRKSTLAIDSMIQKHERDVTFWQSEWSYISEICITLSGAL
                     EQLKNVLTNLIVNEEQMKKNLFLTKGLIVSENVMLELGKIIGRTTAHDLLYELTMRAFE
                     EDKDLKDILLEDNEIKKYFSDEEINSLTNPESYTGLSEKFVDDVYNKWLSYT"
     gene            complement(15312..15443)
                     /locus_tag="BK765_RS32865"
     CDS             complement(15312..15443)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BK765_RS32865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_003310613.1"
                     /transl_table=11
                     /translation="MGKYNKLLSNKEGNTPIEYVRALDTLEEFNELNLDYVETSMKE"
     gene            complement(15695..>17689)
                     /locus_tag="BK765_RS01870"
                     /old_locus_tag="BK765_01780"
     CDS             complement(15695..>17689)
                     /NRPS_PKS="Domain: PCP (173-241). E-value: 4.2e-16. Score:
                     50.9. Matches aSDomain: nrpspksdomains_BK765_RS01870_PCP.1"
                     /NRPS_PKS="Domain: NAD_binding_4 (279-526). E-value:
                     7.1e-49. Score: 158.1. Matches aSDomain:
                     nrpspksdomains_BK765_RS01870_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: NAD_binding_4"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895184.1"
                     /locus_tag="BK765_RS01870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK765_01780"
                     /product="thioester reductase domain-containing protein"
                     /protein_id="WP_142287848.1"
                     /sec_met_domain="AMP-binding (E-value: 3.7e-36, bitscore:
                     114.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.4e-13, bitscore:
                     40.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 2.5e-49, bitscore:
                     158.1, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="TVGELYIGGIALARGYLNQPELTKASFIPHPFKNSSNDRLYRTGD
                     LVKYLPDGNIEYIGRADKQVKIRGFRIELGEIETILGNHPDIKEVTVVAQEDSFGDNIL
                     VAYIVGEGDTQEWRKHVGVHLPNYMVPAHFIKIESLPLTVNGKVDKDALPAWASIIQTN
                     EGYIAPRNRVEQKMVEIWSEVLGIDSSVIGINDNFFDLGGHSLKIMSTLVKTFSEGWNV
                     TIKDYFELKTINNIAKKIQYGYKVNSHSDTLDIKLTTPPKKLKKTKRHEFSNNSKILLT
                     GATGFLGIHLLEQLLDTTECKIYCLVRGENKQEANNRLLHMLKFYFKNKFVSYQSLVNQ
                     RIFIVQGDLAKQNMGLENELYKELHQQISTVIHAAALTKHFGEYSEFERANVQAVKELL
                     AFVGDTKDLHHISTTGVAGQFVLNETETIFKESDFYVKQNYKDNVYVKSKFLAEYEIFK
                     AILTGTDATIYRVGNLTNRYIDGQHQYNFTENAFMSKLKFILQYGIVTDVLLSSEVEFT
                     PVDYCSKIITGFVTSNEIHENSKNCVNHIYNHLTLNLNVLVKLLNSMGYPIKVISETEY
                     QQFILELSKDKKKQEDIQNLMTFEEPKDNCYKPINLDSTETQKTLKLLGIQWPIINIEY
                     IQQIINYMTSVTGSNSVAKLFKQSYTLKN"
     aSDomain        complement(16109..16849)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK765_RS01870_NAD_binding_4.1"
                     /evalue="7.10E-49"
                     /label="BK765_RS01870_NAD_binding_4.1"
                     /locus_tag="BK765_RS01870"
                     /protein_end="526"
                     /protein_start="279"
                     /score="158.1"
                     /tool="antismash"
                     /translation="LTGATGFLGIHLLEQLLDTTECKIYCLVRGENKQEANNRLLHMLK
                     FYFKNKFVSYQSLVNQRIFIVQGDLAKQNMGLENELYKELHQQISTVIHAAALTKHFGE
                     YSEFERANVQAVKELLAFVGDTKDLHHISTTGVAGQFVLNETETIFKESDFYVKQNYKD
                     NVYVKSKFLAEYEIFKAILTGTDATIYRVGNLTNRYIDGQHQYNFTENAFMSKLKFILQ
                     YGIVTDVLLSSEVEFTPVDYCSKII"
     aSModule        complement(16109..17167)
                     /domains="nrpspksdomains_BK765_RS01870_PCP.1"
                     /domains="nrpspksdomains_BK765_RS01870_NAD_binding_4.1"
                     /incomplete
                     /locus_tags="BK765_RS01870"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        complement(16964..17167)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK765_RS01870_PCP.1"
                     /evalue="4.20E-16"
                     /label="BK765_RS01870_PCP.1"
                     /locus_tag="BK765_RS01870"
                     /protein_end="241"
                     /protein_start="173"
                     /score="50.9"
                     /tool="antismash"
                     /translation="EQKMVEIWSEVLGIDSSVIGINDNFFDLGGHSLKIMSTLVKTFSE
                     GWNVTIKDYFELKTINNIAKKIQ"
     CDS_motif       complement(17453..17518)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK765_RS01870_0002"
                     /evalue="1.20E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="BK765_RS01870"
                     /protein_end="78"
                     /protein_start="56"
                     /score="39.6"
                     /tool="antismash"
                     /translation="YIGRADKQVKIRGFRIELGEIE"
     CDS_motif       complement(17606..17683)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK765_RS01870_0001"
                     /evalue="1.90E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="BK765_RS01870"
                     /protein_end="27"
                     /protein_start="1"
                     /score="45.8"
                     /tool="antismash"
                     /translation="VGELYIGGIALARGYLNQPELTKASF"
ORIGIN
        1 attattaaat taggtaataa tatttctaat actgaaaaaa tatttggttt aggtattctt
       61 ctttatattt tagaaacaat taaagtaaga aaaagtagga tttttaatga ttagaattaa
      121 atatttacat tataaaattt attgaatctt ttattaggga ttacatcgct gaaagtaaaa
      181 tattgtatac ttctgaatta ctttacgttc acttcttgaa attgatgagc atttgttatg
      241 ccagaaccaa ctggaaatgg aaacggcaag cgagtatact ccgcttgccg ttttttcata
      301 gcttcgtatg tatttgaatt cctttcgctt tctcttcttc acccttcaca cgtacaatta
      361 aatcatatga tccggatttt ggcgcttcaa aagaaagcaa accttctttt tgagaagtga
      421 cttcttcttc ataaatgacc ttatcgtctt ttgtgacttg caataaaatc gtaccttctt
      481 caactgctgc ctcataagga atttccacat ttcctttttc agctttaagc gtttgtacat
      541 cgtaataccc ctgcaaagaa ccaattgtta aagtcgtttc gccatttttt tgaacctttg
      601 tgacttttgt tgctgtatta catgcgctta acaatacaac aagagtgaaa atgagaaatg
      661 agcgaagtaa tttcatagag agtccccttc ctttaaatat atatgtatta acattcatcc
      721 attaactatt tcaaaaacac taacagctta aaagaatcac cgtttttctt caacttcttt
      781 tttaaataat ttttgaaact ctaattctgt ttcattaata aaaccaagat gtaaaacact
      841 agaaatatct tcatgattca aaaagaaatt atactccgaa gaaatattac catgaggaaa
      901 ataacaacat acataatccc atgttctttt cgtttcagaa tccatctgtt ttcttccata
      961 aaccattaat ggatgttccc aatctttaaa cattacaaca gaacctattg gaagtaattc
     1021 taactctaaa ttttgtacca tcttagtttc cccttcttaa aatagccttt tttataatga
     1081 aataagtgac agaaaacaac aaacaagtac caattgtacc aaaaactgca accgaaatgt
     1141 tacctgctaa tgtttcattt ggatccaatg cattaggtaa aattgtacca aaaattgcaa
     1201 tacaagctaa tataatcata agatatttaa aatttttcac ttttttattc atactaatta
     1261 tctcctttat tccttaacac taacttctaa accaaatccc cataaaccta caccaggacc
     1321 aaatggtgca tggaatttga atccactttt tccagcttca aaagatcccc cgagtgttcc
     1381 aagcgatgca gatcccccaa tttgtaactt tttatccata aatggaatag gaatttttat
     1441 agcactctca tattttacta gagtagcttc agctcctata tgtaaatttc cattttccac
     1501 atgagcacct aactctgcag aagcaagcgc tactttagct tcagctccag ctccaaaaac
     1561 aatatcctct tgttctattc cagcttgagc tactgtaaac ccaactttag gtccataagt
     1621 ttgttccctc ttttctttat cttttacatt aaattgatca taagtacgag aacttacaaa
     1681 tggctctgct tcaagtgtaa gacctttaat aaatatacca ccatatccct tttcctcagt
     1741 aaatgatttt tcatactttt taccatttat tatactacca attcctttta caactactcc
     1801 agtaactgtt gctactccat tagctgtttc ctttacctct tttgaatcca tcaaaaacca
     1861 atcattttta acagcttctt tttcaatttg aacagcatga actttatctt ctgatttttt
     1921 ttgaatagct actgtccatt ccatatttaa ttctttcaca ctaaatatcc ctgttaaagc
     1981 attaaaacct tttccacttt ggacttccgc aagacctgca gcaatactcg ctgctaattg
     2041 aattgctgta ctataattat tactagaggt ctgattgaat tgatacaaat gatccagctt
     2101 ttcttgcagt tttttcctca taacaataaa aagatttacc atggcatcca tacccggcat
     2161 tggcatagct tgattgacag cttccatact tgttttcatt cggtcaattt cccgaatttg
     2221 ttctaatatc tcttgttcaa ttacatctgt ggaagcgact tgtgattgaa attgacttgg
     2281 aaaagcatca ttttgacgga ttaattcttc acacaaataa atgattcctt gtgccaaagg
     2341 tcgaaaggtt tgtacaaaaa atgcttttgc actactatat gtttgccctt gtaaaacagt
     2401 atcaactgca aaagcgtcaa tcgactgaat ggcttgttcc ataccttgaa tagtagcggt
     2461 acatacagca ttcatacttt gagtttggtt ctgtacttct cccaaataca tatttaaact
     2521 catgaattag cctcctttac taattgttgt tgctgatatg aaagggcgtt ttctaaatca
     2581 ctaagatctc gtttctcttt aaacaatgtt tcttttttgt tttccaattc aaatgtgact
     2641 tttcgttcaa tatgttgtgc atcttgatac gtattcataa aaaactggga catttctcta
     2701 tcaccatgcc aagttcctaa aattcgttca aacaaacgac gatttcgact tctacattca
     2761 taaaaatctg cttccacttg ttcttgagct tgaatcgcag attgatttcg gtcttgttct
     2821 tcaaatacac ttcgtaactt ttgatttact tgattaattt gtttttcaat atcttgactc
     2881 atctttttcc tcctactatt ttactttacg taggttgtca cattagcgaa aagtattgtg
     2941 aagttcgtta tccattctct caaattcatt agataccgaa tgaatattat caaccgcttg
     3001 ttgaaaggca gcggaaaatt gtttcgtcaa gtctaacatt tgttgattcg cttcttgtgc
     3061 tttgaagtta acagataatg ttgtacgcgt cgcctttgtt atagaacgag ttgttgcact
     3121 ttgaatccta tcaggggctg atcccatttt cgtagcaatt tgcgttgctg tttgtaaatt
     3181 actttgaaat tgtcccattc tttcaaaccc tttctataag ttttccaaac gcatcaaaga
     3241 aatctcataa aatatatatc cctatcaata tcactcgctc taggcatgca attaaatgag
     3301 ccatacaata taagctgtat ggctcatttg aaatgcttgt tatatagagt atgacaattt
     3361 ttatttcaaa ccgaaattac gtgataattc ttcatcatga gaagcaacag cttctgcagt
     3421 tttattcagc tgcatattaa tttcttgtaa tagctgtgca aagttttgta cttttggctt
     3481 taattcattg aactgttgat caaaaccttc gaaagcacga ccttcccatt ctcctcttaa
     3541 ttcattttgc aagttttgta gttgacttaa aatgtcctca atttgatttg caccttgtcc
     3601 atatcgagcc gctttcgatt taagctcctc tggtgacata cgaatttgtc cagccatttt
     3661 catttcccct ttaatacata atgagcaaaa tatatattag ttaattttac taaatatatt
     3721 ataatgtatt attttgaact tttaaacaat ttatctttct attgaatatt agaatattaa
     3781 tattctactt tttcttgctt tacaagattt tatgcaaaaa agaccaccaa aatgaactga
     3841 ccccataaag ttagacaggt cgtttcatta ggcagccttt agggcatgag ttcgatattg
     3901 taccggactc atgcctttta gtttttcctt aattcttttg tgattataat aatcgatata
     3961 tttttcaaat tcttgtttaa agtgttctat gctttcaaaa tcttttagat aaagaaattc
     4021 agatttcata ataacaaaga aactttcaat aaccgcatta tcgtaacagt tccctttacg
     4081 agatatactt tgcgtaatct tgcgttcctt tagggcatga cgatacttct tcatttgata
     4141 atgccagcct tgatcagagt gaataaggag ttcgtcttct tctgtcaatt gttgaaaaga
     4201 cttctccaac atctttgata caagtgaata ggttggtctt gaatcaattg tataagcgat
     4261 tatctctcca ttaaatagat ctaacatcgg tgataagtag agcttctcgc cacataattt
     4321 aaattctgtt atgtctgtaa cccatttttc gttcggcttt tcagctttaa aattacgttc
     4381 taaaatattt ggcgcaatct tacctacttg tcctttatac gagcgatatt ttttcatacg
     4441 aactagactc tttaaaccta actctttcat aattcgttga acttttttat ggttgatttt
     4501 gtgtccttcg ttcattaact catcgcgaat acggcgatat ccataacgac cttggtgttc
     4561 atcgtaaatg ttttgaatta aggatcttta gctctttatc tttatctggt cgattagaat
     4621 tctgtatcca gtaataatat gtacttcgtg ggagttctgc taattttaaa agtgcctgca
     4681 ccggaaatgc tttccttagt tcatagatta cttgcgcctt atcttgtttg gtgatttttc
     4741 cttgttttga actaaggcat tcaacttttt taaataagca ttctccatac gcaaacgctc
     4801 caattcagct tgtaatgttt ctactgactc ttctgtttgt gtttgtttct taattgattt
     4861 ttgattgtcc ttagtcatag attgacgccc ctttttcttc gatttaaggg cgtctactcc
     4921 tttttgatgt agctgaatac gccattttct aataagtcca ggtgaagaaa tattaaaaat
     4981 gactgccgtt tcgtttggag acgtcccatt ttcgaacata taatttagta cgtctagttt
     5041 atactgtgca gaatacgttg tatagccttt ttcaaaggcc cggattccat gatattcaaa
     5101 ttgttttatc cacatgcgaa ccacttcgtg attcgctccc aatgatttcg caatatcttt
     5161 tatactctca cttccatctt gataatgtat tactgcattc attttgtctt ctgaagtaaa
     5221 tttagtcata aaaaaactac acctccaatt gttagactgt gtctaacaat tggggtgcag
     5281 tttttttatt gtttcttgca agtaaattat ttatattcct gtctatataa tttaaacgaa
     5341 taaatagctc caataaaaaa tacaaaagct tcaaataata taaagatttg acggaaattc
     5401 tcaatatctt taccaactaa agaaataaat atagcattca ttagaatttg agtagtaaac
     5461 atacgagaag taatttttaa ataaatagca tttgtgcttt catctggttt accaattttt
     5521 ttgtaaataa taataagtgc aattactgaa cccaaaaata gaagtgctgt aattcctaca
     5581 acgatattga aattgcctgt actttctaac atccatttat ttaatagatc aatcattatt
     5641 atcttccttt cctacataag agaatatttc gtccttcttt ttcactataa taaaaaaaga
     5701 aaaaggaaaa catcgaactt tcttacaaac attcgtgtat tcttacagtt ttgtcatttt
     5761 gtaaatgtat tcatcgcctt gcctttcact attttctttg gaaaacatag tgaattacga
     5821 gaaagagcgc tacaagatca acttccaagg agtagtgcac taaaactctt aattaatgca
     5881 attagtttgt tttaaagtag taagtggaca caatacttct tagttgtaga aagtatcgag
     5941 atccctgtgc caattccagc gtttgctgca gctctattaa ttggtttagg aataatcccg
     6001 ctttgttctt aatttaagga actccaaaca gccctacctt tcaattgata aaccctcaag
     6061 tgtatgaatg cttggaggtt tcttataaaa aaaagacgcc ataaggcgcc cccacaattg
     6121 ggtagataag actttagttt tccaatctaa agaagttttg tatcagctct ttgagagtat
     6181 atcataatat tcggttcatt tattatgtat ttttacatac ctataaaaat ccctctattt
     6241 tccacttaat aaaatgaatt accaatatta ataagattca aataaacatt tctacaaatt
     6301 caaaacattt acaaaataag aaaagtgata tatttaaggt gcaagttata aaaaatatta
     6361 aaggggattt attatgttca agaaattagt agttggagca ttagcaactg gtattagctt
     6421 aattgttagt gcatttggaa caccgtttaa taataggatg caggtgttag ccatgttaca
     6481 tagtaaattc gttgaagatt cggttctggt gcaaaaatgt tagttggaaa catagaatag
     6541 caattttctc ttcatttcta tcttatgaag ctaagccaaa ccatttagga ataaattctt
     6601 tctgattttg gacagacttg tccggtaaaa caagttgccc gttttttatc atatgcatag
     6661 attcgacacc actcaatata gagattgctg ttttaaatga cttgaatctc tacaggatag
     6721 gcagggttct tatctactgt tatcacacga ggtttagaaa catgcgaaaa agccaaggct
     6781 ttcttgaaaa agcgcttgac tgctggttta tctcttgatt tacttagata aaaatcaatt
     6841 gtattaccct ctgaatcaac tgcacaatat aactatatcc attgaccttt tacttttctt
     6901 tcaacttagg tcttatatct ttctgtaacg tttgtgaggg atttaagtgt caattctttg
     6961 tacgataaat ttcaatgtta aaatccgctt ttttcttttt tcttttcttc agaccctctt
     7021 attgattctt ctaaacactt caataatacg tcttcacttt tgcatcgaat agcttcataa
     7081 attttaggtc caatgttctc aggcaattgc ctaactgcaa catagaatcc actaaacaac
     7141 atttctaatt cacttgctgt aaactcatac ttctttaact cgccttccga tttgcgtaca
     7201 gattcattca ccagagcatt ctgtgttata tctaacaaac atattaaata atcgtataaa
     7261 cttgcatttt cttatatata ttcaataact tgcaaacgtt cattaggaaa tcttctcaac
     7321 gcttccattt ttatctgtct ataactcgga gtgtgctcat gaatatctgt taaaaactct
     7381 tcagtgctca taactcttcc ccctacatac tcatgttata tattttaaca tgtataactc
     7441 gtcagaaata gaaacattta tttagaatgc taacggataa ccatttttaa tttaatccca
     7501 tagatgaggt ctttttaaac ctaaagccct catatctctt aataattgcc ctgtatgtac
     7561 ggcttcatga tacgccattc tcattaacat atctccaagg tttctagtat atcctacatc
     7621 tgagcgatct atttttatat tttcaagatc ttcctcattt aatcccatta taaaatctaa
     7681 gaaatttttt cgataaggtt ttgcaaaatc aacctcatct tgaatggttg taaaatcgcg
     7741 tgtattgaaa ggatttggtt catcatcgct atagggcaaa ctttttctag tctttaaaat
     7801 ttcaagatat aaatattcag cttctaaaac atgccgtact tgttctaata tagtcattga
     7861 agatgaatca accctatgaa ataaaacctc actgggaata gaactccata aatttaaact
     7921 tctccggcgt acttcattag aattcataat tattagttca attttgttca taaaataact
     7981 ccctttacac ttaactaatt acaatgctgt atcattttga atcttatcta atctttttct
     8041 atcaaataaa ccaagactta cacctacgat tgatgatact atcgcgaaag cacctgcaac
     8101 taagaagatt gacaaaacat ttattgactc agccaaaaag ccagctatca tagcactaaa
     8161 aggtaaagct aaaaatgcta ccattctagt agaactgata actcttccga gcaacttgct
     8221 aggtacagtc tcttgtcgca atgtaaacca attaatagct aataaagtaa ctgatccttt
     8281 aataaaaatt tgacagaata ctaaaattac taagctgaat tgagagaagt acaggagcat
     8341 ctgggcaagt cctataacaa tccctgaata aataataaca tttcctcttg gaatatgttt
     8401 aaatttactt gcaaaataaa cagccaaaat tggtccaatt gcctgtaatg aaaaaattaa
     8461 tccaatattt ataggactca agcctagata atctttcaag taatacataa aaagagattc
     8521 tacagctcca aatcctatat taataccaaa aataactaga gaaccaacta agataatttt
     8581 cgttccaaag acatatctta gtccttcaac aataggtttt actaattcag ggcgtttctt
     8641 ctcttcttca gcaaaatctc taaattttaa aaataaaaat attgttcctg ataaaaagaa
     8701 ggccacactt gttattaata aaacattcga attcccaaaa agcgctattg caatccccgc
     8761 tacggcgggt cccaagaatg taataatcgt ttcgcttagt ctaaagaatg agttaccttg
     8821 aataagatcc tctttagata agatttcagg aactgcagat tcaaaactag gatgatgaaa
     8881 agctgctaaa gcagataagg taaaagcggc aatatatata actattatgc tgttataacc
     8941 acttaaaaaa agtataggaa ctacgagtac aaaaagcgct gaagcatagt ctcctattat
     9001 cattatcttc tttttagaca tttggtctgc taatgcccca cctattaaac taaaaataac
     9061 ttgggctcca agctctaatg ctagtacgaa ggccatttta cttggagaat gggttaaatc
     9121 ataaacaatt aatggaattg ctatagtgaa gaaccacatt cctaatttac ttattgtcat
     9181 cccagaaaaa agtattacat aattcttatt taaaaaaact ttaggtagca tatcaaatca
     9241 cttcacttca cttttaacta cttgaccaca gagaggacaa tctaacattt tttcccacga
     9301 agaaacagta ttagtttcta gggttaaaaa gtccacttcc ataactttcc ctaaagaaat
     9361 aggttgtgca atctgcgtta aaatttttaa tgcttcatta acaattgttc cagctacaat
     9421 agctacgttt ggacttatag cagcattgtt tctagtaaac tccatgttat tcatagcttc
     9481 taattgctcg tcttgtttat cgtttaattg tcttgcataa ttaatgtgac attgtacaca
     9541 acctgtctta gaaggaatca tagaaaagaa tctaccacga gtatttaata ctccaccata
     9601 aaccattggc agattacatt taaccacacc ttcgtttacc cagtttgcaa tatgtaatgt
     9661 tggcttatct gcaacacata tcacaagctc agttccttca atatgttcaa tcacatcttg
     9721 aggtgactct attttcttat ttataatttg aagatccaca ttagggttga attgggatat
     9781 tcgcttttta gccatttcaa ccttactatt ccctatatct gattccgaat aaagcaactg
     9841 tctattaaaa ttacttaatt caatattatc aaagtccaga gcttttatat tatgaaatcc
     9901 taatgctgcc aaatgataca gtacttgtgt tccaagtcct cctacaccaa gaagtgcaat
     9961 aggcgtttct aaaatctttt cttgaatttt atatttatct tctccgaatt tcgtgaatag
    10021 tgaaaaataa tttatattcg ctttatatct ttctaagatg taatcattga gttctggtac
    10081 aattgaatta tcttctaaat aaccttcatt tcctaaaact tttatagcct caataatttc
    10141 atttgaacta atatcaggaa attctttttg tacttcttga acgatgtttg gaatgtttct
    10201 ctggccatct aatttattta ttagaaatga gatgctacca gtagggtctg gaattatacc
    10261 tgctaaacct tcttcttcac caatttgaat ttctttattt aggaaaaaat aagggatagt
    10321 ttctttaaat tttggtttca ttttataaca tctccttaat taattgatta attaaaaaat
    10381 taagaagaac cggaaatccg gttcttctta atgacttaac ctggtacatt agtaagtgca
    10441 ttagcagcaa ctactttttt agaagctttt tttactttaa cgatcatatg taatcacctc
    10501 ctacatttga taaatctagc ataacacaca aaaaagagaa attacataat agtctgatag
    10561 tttcaggttg agaaactttt ttttgaaaat tatagaaata actattttta gtagtttatt
    10621 cacctgtaaa aaatcaatcc ataagcaagt ttaagtcttg ataggttaag tttccatttg
    10681 aatgtaataa tttcccctgt ttaaaggtgc tgcttatctt atcaaggttt gttagaatat
    10741 tcctaaaagc tgaagatcca agactcactc tctcaatttt taatgtcttt atgttttgca
    10801 tggaatttaa gttttcactt tgaagcaaat ttataggtaa ctctatattt tctctaagta
    10861 cttttattgt ctcaatatcc ttaactccag gaataaaaat acaatctgct ccggattttt
    10921 tatattcttc tgcacgaacg attacatcct taatacgatc ttttaaatca ccaatatcta
    10981 gccaaaaact atcaattctt gcatttatga aaagatgagg cgaataagct ttaataaagt
    11041 ctaaaatgac cttttgctca ttaatttctc ttaaatacgt acctctccca tcctctatat
    11101 ttatacctac cacttcaaat ttttctaact cgtttaatat ttttcgtaaa tcaaaaatat
    11161 tagcggcata tcctccttca atatcagctg aaacaggaag tttggtattt ttcgagatat
    11221 tactaatgga atttaacatt aactctaaag gaatcttctc gccatctgga taacctaagg
    11281 ttgctgcgat accagcacta gtagtcccaa tcgcttgaaa atcgtttctc tcaaaaatct
    11341 tagcactcga tgcatcccat gcatttggaa gcattaatgg attgttttga tcatgtaacg
    11401 ttcgaaataa cttaattttc tctttaaatt ccaatttaaa caccctttag taaattattt
    11461 ttttagtttt acacttgact ttttttgcac ctactagtta tgttgtttta cataacggat
    11521 taaattagat cttgcagaaa ggataggtgt gagtatatga aaattgcgat agtaggttta
    11581 ggaccaagag gtatatcagt atttgaaaga atagtctcta atattttatc tgaattaaaa
    11641 tcagaatctt atgaaatttc cttggttgat ccacatcctc caggtggagc tgtatggaga
    11701 acagaccagt cacctcaact tttaatgaat acagtggctt cgcaaataac tttatttact
    11761 gatgatagcg tccagtgtcg gggtcctatc gttaatgggc ctagtctatt agaatgggca
    11821 aatgaattaa gggataaaat tgacactttg gatatgccca ccgttattac agaagaaatt
    11881 tattccttag aacctaactc ttaccctaca agagctctat acggctatta tcttaaagat
    11941 tcatttaata ctattcttga agaattaccc tcaaatatta agattaactt aattcaagac
    12001 acattaatta atctctatcc gaacaatgaa gattatactt tatttcttaa taagtcttta
    12061 acaccaatta aagctgaaaa ggttatcttt acaactgggc actcagatca gaaattaact
    12121 tcagctgaag aacatttctt tgagtttgct aatagtaaca acctaaatta cttcccacca
    12181 tccaatccag cagatgttaa tttagattca atagaaccta aaactcccgt aataataagg
    12241 ggattaggcc ttaatttttt tgattacatg tcacttttaa caagtgggag agatggaaag
    12301 tttgaagaaa ttaatgacaa attaaattac attccctctg gtaaagaacc tttattaatt
    12361 gcaggttcta ggcgaggctt accttatcac tctaggggac aaaatcaaaa aggttcttca
    12421 gaaagacata tcccactatt tctaaaaaat gaagttatcg agcagttaaa acataaaaat
    12481 aacctaagct ttaaaaaaga gatatggcct ttcgtaaaat tggaagttga aagtatctat
    12541 tatacaactt taattcgcaa tctctatgga atagaaaaat cttgtcagtt cctttacgag
    12601 tttgttaatt gtgccataga ataccataag gatcttttaa tcaactttgg agtaaaatca
    12661 gaactatttt ttgattggca caaaattagt tgtccttatg gtgataaaaa atttaattca
    12721 ataacagact ttcataattg gttagatact tacttacata atgatattaa cgaagcaaac
    12781 ctgggtaata aaaacagtcc actaaaagcc gcattagatg ttttaaggga tatccgaaac
    12841 gaaataagaa tacttgttga tcatggagga ataaatgctg aatcttataa agaagagtta
    12901 cgcaattggt atacaccttt aaacgcattt ctttcaattg gccctcctgt tcaaagaatc
    12961 aaagaattga gggcattaat aagagccgga attttagttt tagttggacc tgaagcaact
    13021 gtagctactt cacctataaa taaagatttt aaaattagct ctaaccgtgt taaggattcg
    13081 atatttcatg caaagaacct aattgatgct agactaccct cagttaatat aaatcaaagt
    13141 aaaaatattt taatctcaaa tatgttaaaa gcaggtctag cctcagaatt taagattgca
    13201 acgaacaata attatatttt tgaaagtgga ggtttagcag tgactccccc cccaaataga
    13261 ttaatagata aaaacggtaa agttaatcct aatttatact cctttggaat tccaacagaa
    13321 ggcgttcatt gggtaactgc tgcgggagcg cgaccagggg ttaattctgt aacactcctt
    13381 gaatccgatt caattgcaag agatgttcta ggctgcaata ttacaattaa taatgtgtat
    13441 aagcagacca ttagctgagg aattattcct cagctaatgg tcttaaattg gttttatgta
    13501 taactcaacc atttgttata aacatcatca acaaactttt cactcaaccc tgtgtatgat
    13561 tctggattag taagggaatt tatctcttca tcgctaaaat acttcttaat ttcattgtct
    13621 tctaataaaa tatcttttaa atctttgtct tcctcaaaag ctctcattgt taattcgtac
    13681 aataaatcat gggcagtagt tctaccaatt atcttcccca attctaacat tacgttctca
    13741 gatactatta aacctttggt aagaaaaaga ttctttttca tttgttcttc atttactatt
    13801 aagtttgtta agacattttt aagttgttct aatgcgccgc ttaaagttat gcaaatttcc
    13861 gaaatataag accattcaga ttgccaaaat gtaacatctc tttcgtgttt ctgaatcata
    13921 ctgtcaatag ctaaagttga ttttctattg actattttag ttaatcccac aatataagag
    13981 caagtcatag gatttctttt atgcggcata gtgctacttc ctactttacc agaatggtac
    14041 ccctcttcta cttctctaat ctctgttcct tgaaggctta ttatttcttc tgcaacttta
    14101 gctgtagttg ctccaatcat atttaaaatc atgctaaatt ctgcaaatcg gtctctagac
    14161 gtgtgccaag atactaaggg ttcaattagg tttaaatttt ttacatactc ttttctaaca
    14221 gctaaccctt gcgctcctaa tgaagctaaa gtaccaactg cacctcccag ttgccccact
    14281 aaatatcggt ctgcaccttg ttgtaacctt tctaagtgac gaccaagttc atctgaccat
    14341 atagatatct tatatccaaa agtaatcggt aaggcatgct gacctaacgt tctgcctggc
    14401 attactgttg acttatattg ctggcttaat ctcaaacata tctttaatat ttcttgagtt
    14461 tgattataaa taagttcatg agcaccttta atttgcaggg taagtcccgt gtcaattata
    14521 tcttgtgttg ttgctcccca atgaatgtac ttacgtgatt cttcatcaca taaactctcg
    14581 aactcatgta ggaatgctac aaaggggtgt ccagtttttt tcattccttc tctaatctta
    14641 ttaaaatcaa gtttattttg gtcagctgca tttgcaatat tagtcgctgc attttgaggt
    14701 ataataccta atttagcttc agcttttgct aaggcaactt ctgaattaag ccacaattgt
    14761 attaaattct cgtctgaaaa atttgttctc atttgttttg taccaaactg gtccttaaag
    14821 atttcagaat caattggtgt cgaattcatt agtatcacct ttctttttca ttaatttaag
    14881 ataaaatatc tactattgat attagcatat taatgtaagt aatttgtctt aaaattgatt
    14941 ttatatattt ttgaaaccta aaatgaaaag gaatacggga acaaaagaac aaaaaaacac
    15001 aacagataaa agattctttt cgtaacaaac gaagggatct tttatctgtt gtaagaacca
    15061 tgggttccca agaatccttc acctatcatt tattcatttc ctgttcccaa ttatcaactt
    15121 aaatatccgc tactccctgt tccaatggct acacatctcc ttttgttact gtagcttgtt
    15181 catataacgt gtttaacgat gttattcccc ataaagaaaa agataatcca cttaaaattg
    15241 taatctcgat gacagttttc cccttacctt atataacgta gaaagctgaa aaagtacttg
    15301 ttatattgtt attattcctt catactcgtc tctacatagt ctaaattcaa ttcattaaat
    15361 tcttctaatg tatctaacgc tctaacgtat tctatggggg tatttccttc tttgttggat
    15421 aatagcttgt tgtattttcc catctgtttg caatttttcg acagcaatgt ctcatacgat
    15481 atgaaaaact ctactagggt gtcttttacc caatattcaa aaggttacat gtgtttgctg
    15541 actaaaaatt atttccttat aaaggataga atcacaataa ttatgcatat aattccgcct
    15601 aaaagcttgt cctatttcag tgattttgtc taaaaaattt tgcgtatttt tttaactgtg
    15661 ttcctttaaa atttctcatt tctcatgagt ctgttcagtt ttttaaagtg taactttgtt
    15721 taaaaagctt tgcaacagaa ttactaccag taacactcgt catataatta ataatttgtt
    15781 gtatatattc aatattaata ataggccatt gaatacctaa taattttagt gttttttgag
    15841 tttcagtcga atcaagattt attggtttat aacaattatc ttttggttct tcaaatgtca
    15901 tgaggttttg aatatcttcc tgtttctttt tatctttaga aagttctaaa ataaattgtt
    15961 gatactctgt ttcagaaatt actttaattg gatatcccat tgaatttagt aattttacaa
    16021 gtacatttaa atttaacgta agatgattgt atatatgatt tacgcaattt ttgctatttt
    16081 catgtatttc atttgaagtt acaaaccctg tgataatttt gctgcaataa tctacaggtg
    16141 taaactcaac ttcacttgat aataatacat ctgtcacgat accatattgt aatataaatt
    16201 ttaatttgct cataaatgca ttttcggtga agttatattg atgttgtcca tcaatatatc
    16261 tatttgtaag atttcctact ctataaatag tagcatctgt tcccgttaat attgccttga
    16321 atatttcata ttccgccaaa aatttacttt tcacatacac attatctttg taattttgtt
    16381 ttacataaaa atcgctttct ttaaatattg tttccgtttc atttaataca aattgtccgg
    16441 ctacaccagt tgtagatata tgatgtaaat ccttagtatc accaacaaat gcaagaagtt
    16501 cttttaccgc ctggacattt gctctttcaa attcactata ctcgccaaaa tgttttgtta
    16561 aagctgcagc atgaataaca gttgatattt gttgatgaag ctccttatat aattcgtttt
    16621 ctaatcccat attttgttta gctaaatcac cttgtacaat aaagatacgt tgatttacta
    16681 aagattgata gcttacaaat ttatttttaa aataaaattt taacatgtgt aataaacgat
    16741 tatttgcctc ttgtttattt tccccacgaa ctaaacaata tatcttacat tctgtagtat
    16801 ccaatagttg ttctagtagg tgaataccta agaatcctgt agcacctgtt aaaagtattt
    16861 tgctattatt actaaattcg tgtctttttg ttttctttag cttctttgga ggagttgtca
    16921 atttaatatc taatgtatct gaatgtgaat ttactttata tccatattga atttttttag
    16981 ctatattatt aattgttttt aattcaaagt aatctttaat agttacattc caaccttcag
    17041 aaaatgtttt tactaaagta ctcataattt ttaaagagtg cccacctaga tcaaaaaaat
    17101 tatcatttat cccaatgaca gaggagtcaa tccctaatac ctctgaccaa atttctacca
    17161 ttttttgctc tactctattc cgaggtgcta tatatccctc gttcgtttgt ataatagagg
    17221 cccatgcagg taaagcatct ttatctacct tcccattaac agttaatggc aatgattcta
    17281 tcttgataaa atgagccggg accatataat ttggaagatg tacccctaca tgttttctcc
    17341 actcttgtgt atctccttcg cccacaatat aggctactaa gatgttatct ccaaatgaat
    17401 cttcttgcgc taccacagta acttctttaa tgtctggatg atttcctaat attgtttcaa
    17461 tttcacctaa ctcaattcga aatcctctaa tcttaacttg tttatcagct cttcctatat
    17521 attctatatt tccatcaggt aaatatttaa ctaaatcacc agtccgatat aatctatcat
    17581 tagagctatt cttgaatggg tgtgggataa atgaggcctt tgttaattca ggttgattca
    17641 aatatcctcg tgctaatgcg atacctccta tataaagttc acctactgt
//
