LOCUS       NZ_MOOL01000084        12247 bp    DNA     linear   CON 03-APR-2024
DEFINITION  Bacillus thuringiensis serovar xiaguangiensis strain BGSC 4BN1
            contig000084, whole genome shotgun sequence.
ACCESSION   NZ_MOOL01000084
VERSION     NZ_MOOL01000084.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis serovar xiaguangiensis
  ORGANISM  Bacillus thuringiensis serovar xiaguangiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 32048)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Comparative genomics of Bacillus thuringiensis reveals a path to
            pathogens against multiple invertebrate hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 32048)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, 1st Shizishan,
            Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.5.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002147905.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 02:08:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,596
            CDSs (total)                      :: 6,434
            Genes (coding)                    :: 6,198
            CDSs (with protein)               :: 6,198
            Genes (RNA)                       :: 162
            rRNAs                             :: 15, 15, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 1 (5S, 23S)
            partial rRNAs                     :: 15, 12 (16S, 23S)
            tRNAs                             :: 114
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 95 of 236
            Pseudo Genes (incomplete)         :: 153 of 236
            Pseudo Genes (internal stop)      :: 70 of 236
            Pseudo Genes (multiple problems)  :: 63 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:39:27
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 320
            Orig. end                         :: 12567
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOOL01000084.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12247
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12247
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12247
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:7247](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7247)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    149..163
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 18.24"
                     /tool="antismash"
     misc_feature    409..422
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.06"
                     /tool="antismash"
     gene            complement(433..861)
                     /locus_tag="BK733_RS33820"
                     /old_locus_tag="BK733_32045"
     CDS             complement(433..861)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BK733_RS33820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BK733_32045"
                     /product="hypothetical protein"
                     /protein_id="WP_088021669.1"
                     /transl_table=11
                     /translation="MIWRQEEVGRKDQVIFEGDWLWFEMTSSNWDGYRYFNFPESRGYV
                     KLDKTLSTLSIRDHNNGNYVVKTGGQSTVCNDTIGRPTDCFRADHSYTADSEFYLFPAG
                     GIDENKNWLTIFPELHRKPWDLSYSEIQPFVFIPVDNK"
     gene            complement(1207..2079)
                     /locus_tag="BK733_RS33825"
                     /old_locus_tag="BK733_32050"
     CDS             complement(1207..2079)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748173.1"
                     /locus_tag="BK733_RS33825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_32050"
                     /product="hypothetical protein"
                     /protein_id="WP_088021670.1"
                     /transl_table=11
                     /translation="MKKGIEFLILITIIIYINNFAFAYINGYKTLIGVSALWAISPFLF
                     LTIASFILANDYKSDYSIVKKEARVDFALKVISCMVAFYNYKFEIGSMEYIMRFVILAI
                     LFIGNIILEYKMYKVAKNYVPKISNEIKTITEKEKWNIKNYGRAATLGLGSFMLVVVGG
                     MNVVYITNMNKFYSLISICIFVIFLKMNYDKNNLFHRDKVVGKQIFLRDAFYASLGFGY
                     NLVVAFDIIPSSEMVVNTSLIIGVFFLYPTIVTNRKIALKQREVSKVIGDNFAYYYNDD
                     NNPYKNYNF"
     gene            complement(2177..2518)
                     /locus_tag="BK733_RS33830"
                     /old_locus_tag="BK733_32055"
     CDS             complement(2177..2518)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067994.1"
                     /locus_tag="BK733_RS33830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_32055"
                     /product="hypothetical protein"
                     /protein_id="WP_088021671.1"
                     /transl_table=11
                     /translation="MSNLVLPSNYVEIENEEMEYVDGGYYISNSTLKGIVFSGVGATTG
                     VSVVAIEGSIYGIAAAMASTIPALGWVTGAVLAANATNFAITATRAISSGKGMDIGVGF
                     PTGLTFAVA"
     gene            complement(2849..3190)
                     /locus_tag="BK733_RS33835"
                     /old_locus_tag="BK733_32060"
     CDS             complement(2849..3190)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067993.1"
                     /locus_tag="BK733_RS33835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_32060"
                     /product="hypothetical protein"
                     /protein_id="WP_088021672.1"
                     /transl_table=11
                     /translation="MSNLVLPSNYVEIENEEMEYVDGGYYISNSTLKGIVFSGVGATTG
                     VSVVAIEAGIYGIAAAMASAVPALGWVTGAVLAANATNFALAATQAISRGKGIDVGVGF
                     PTGLTFAVK"
     gene            complement(3402..4523)
                     /locus_tag="BK733_RS33840"
                     /old_locus_tag="BK733_32065"
     CDS             complement(3402..4523)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /locus_tag="BK733_RS33840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_32065"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_088021673.1"
                     /transl_table=11
                     /translation="MKIYNLNEITDSRIIYDKNPPRFMLYIISIVIILLITIITWSTYS
                     IKTYVVKGQGIVTAQNKHNIMSPVSGAVEEIHVEEGKRVKKGELLLSLKPVQSNLQIEQ
                     LDGQLKHLNERIELLERAQKNVSRGTNNFDKKKSRELEFYNKLNSLQVKMKEFEVKKVT
                     EDSLKEQNYNEEQIKDYKNKAINKMEQEKNEVLLEFTNEKKQIEIEINKLESQKNALVK
                     SLEEYKIFATEDGKFHLSNKITMGMVIQAGALIGSIAEENENFIIELNLPSTERAKVEI
                     KDDVEMVVAGLSQSEYGVVEGEIVSIAEDAILDEKKESNNIYFKVHIKPKQKFLKDSND
                     NIVNLSLGMLTEVRIKYEKITYLDYALEQIGIK"
     gene            complement(4541..5014)
                     /locus_tag="BK733_RS33845"
                     /old_locus_tag="BK733_32070"
     CDS             complement(4541..5014)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268611.1"
                     /locus_tag="BK733_RS33845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_32070"
                     /product="hypothetical protein"
                     /protein_id="WP_254907466.1"
                     /transl_table=11
                     /translation="MSTVEKISITVKKTLKLNNVLIGKVKKNEPLEIQKTSHMMQSYVK
                     SHGKSIVGPLISHSYSSVDSEGNIHFNMNLIVQLDSPHHISDVNYEFKKQLIVENCLFA
                     RFNDEEENIKFAYLKLNVHAFEHNLNLQGESYTVYMKQKSDKLMADIFMPLQD"
     gene            complement(5001..7247)
                     /locus_tag="BK733_RS33850"
                     /old_locus_tag="BK733_32075"
     CDS             complement(5001..7247)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258271.1"
                     /locus_tag="BK733_RS33850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_32075"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_088021674.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.8e-40, bitscore:
                     128.8, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 5e-182, bitscore:
                     597.8, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFSYLVKQHDATDCAAACLATVCMYYKKDISITKLRDILGTDIKG
                     TTLSGLESGAKKLGFDAKAVRVNRETFTSTFTLPAIAHVITQEGLTHFVVVHKITKHFV
                     VILDPAKGKQKISIVEFFSSFDGILLLLAPNNEFVVESTKNKNTLYTFIKLLIPQKMLF
                     VYSIIASLLLTILGVSSAFFNKVLMDEILPYNLKNELTAFGLGFLFVVIIQVAISTIRQ
                     HVLLYLSQKIDIQLLLGFFRHTYKLPMEFFSTRKVGDILTRFSDAFTIKNILTSVTLSL
                     FIDLFLAIASAVVLYFMNNTLFGVVLLLTLVNTILVFIFKGPYKKINFRKKESDSILNS
                     HIVETLKGIETIKIHAAEEKTLEKLEIEYVKNLRISFKEGVLSNIQNTFSSSVSGIGQI
                     LIMCIGTSMVMDKDITLGGLMAFVTLSGYFMDPIGRLIHIQLTIQEASVSLARISELYD
                     VEKEQVNDKKKKVEGLSGDIRLENITYRYGSRSPVLKNVSITIPEGKRVALVGGSGSGK
                     TTITKLILRYFSPEEGNIKISETDIDDLDLYSYRRNISYIPQNIELFSGTIRENVSLGI
                     TGVTDEQLKKACEDAGCKKFIEKLPGKYDTYLDEAGGGLSGGEKQKIALARALILNNKF
                     TILDEATSNLDFISEANVYNTLFSKKKKDSTMLIIAHRLSTIQNCDNIYVLDQGEIVEE
                     GTHQTMMENKGTYYNLYVSQVGGSVTEEGSVENLEAQIEEDTKKYIGDEIIEYS"
     misc_feature    7370..7384
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 20.21"
                     /tool="antismash"
     gene            complement(7432..8427)
                     /locus_tag="BK733_RS33855"
                     /old_locus_tag="BK733_32080"
     CDS             complement(7432..8427)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165817.1"
                     /locus_tag="BK733_RS33855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_32080"
                     /product="DUF4046 domain-containing protein"
                     /protein_id="WP_088021675.1"
                     /transl_table=11
                     /translation="MQVQIITIEEIYQEILDGKRSRFPPGTWNLDNNNEMAKRVTKHLV
                     EKILKWNKKEIKQNWNTSLLVKYRLRGMLQFKYENSPYKMLDDAYPKQYKEWELGMTPL
                     NFWTKEKALQILKWTIEVKIKLTFEDLLKVYGQKWLKEQRLGAPLRTIWNGSPYAMIND
                     LYPDIFKEWQFSMVPNKFWTKEKALKALKWTIEKKEVLTPEQLRLMYGHKWLVKSGLKG
                     ACQLFWNDSPYAMINDLYPNQFKEWEFKMTPTRFWTRERALEALRWTIEEKEKLTDDQL
                     FKYYTVKWIKKNRLWTPLMRYWNGSPYAMINDLYPYRYSKSMFRGYIKYR"
     misc_feature    8822..8838
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 22.07"
                     /tool="antismash"
     misc_feature    complement(8827..8845)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 26.05"
                     /tool="antismash"
     gene            <9653..9769
                     /locus_tag="BK733_RS34530"
                     /pseudo=""
     CDS             <9653..9769
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606463.1"
                     /locus_tag="BK733_RS34530"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="LYHLYFPRLWTHSYFVSISGNVSSATIKRYVDSHISYI"
     gene            complement(9914..10138)
                     /locus_tag="BK733_RS33865"
                     /old_locus_tag="BK733_32090"
     CDS             complement(9914..10138)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016136195.1"
                     /locus_tag="BK733_RS33865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_32090"
                     /product="hemolysin XhlA family protein"
                     /protein_id="WP_140157273.1"
                     /transl_table=11
                     /translation="MEDLYAKIVDLKTGQKEIMQDIRNLETRTTISEKDIAMLNKQLEK
                     INLNTTWILRIVISSVIMAVLGMVLKGVI"
     misc_feature    10446..10459
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.57"
                     /tool="antismash"
     gene            <10489..11250
                     /locus_tag="BK733_RS33870"
                     /old_locus_tag="BK733_32095"
                     /pseudo=""
     CDS             <10489..11250
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520263.1"
                     /locus_tag="BK733_RS33870"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_32095"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="ARKAKFIEAVLQEMNIGELSADMNKFIHVLKHTCHRQIRSVIRGL
                     RDMVDRKEGYPTKIVYTLKKLLHQTSQYQILDTAAKEGIYPLIAQHIPKERNSDREQAV
                     FNFGLHYSMYSLGNIKKMFKNVHALLKQKFAVPVTEESYHRNYLKYQEETLFRKYAYDQ
                     GVNLHAYIALEIEMREKLKVRGHKEHTIPSDVREWFIEAIDKLPQEKLRVIKLPKQFNL
                     LEFMRTFERLVRAGVTITASDEVLTAMEIQK"
ORIGIN
        1 gtctaattca ggacattact gatgaaccaa acgcataatc gttgtatgtg aaatggataa
       61 gccccgttcc tccatcattt ccactacatc acgaaaacta aggttgtacc gtagatacca
      121 tcttaccgtt aacaacatta tatcgggctg ataatgctcc catttgaaaa aatttccttc
      181 tccatactta tcacacgcct tttctagagt agtaatgtca gtatgcccaa gtttaagaga
      241 ttgtttgcgc cagaacctga aatttcatat tttctcgaaa aacaaaaata cctgcagggt
      301 tttaaattat ctacaggtat acaaaaaata ttttaaaaac tctattctta caagtgaaca
      361 tgtctaattc actatactaa aaacactcaa atcccccctt taattttagg catattatct
      421 gcctaaagaa tactatttat tatctacagg tataaacaca aaaggttgaa tctcactata
      481 acttaaatcc catggctttc gatgtaactc agggaaaatc gtaagccaat ttttattttc
      541 atctattcct cccgcaggaa ataaatagaa ctcagaatca gctgtataag aatgatcggc
      601 cctaaaacaa tcggttggtc taccgattgt atcgttacaa actgtagatt gtccgcctgt
      661 ttttacaaca taatttccat tattatgatc tctaatcgat aaagtagata gggtcttatc
      721 taactttaca tatcctctac tttctggaaa attaaaatat cgatatccgt cccaatttga
      781 agacgtcatt tcaaaccaaa gccaatcacc ttcaaaaata acttgatctt ttcttcccac
      841 ctcttcttga cgccaaatca taataggagt ccctaattgt tattttttat ccgtaggcaa
      901 tgcttgtaat acataatcct tattaagata atcttcatat gtcattccta attctgggcg
      961 cccctttaca gacatataat aattttcccc tgtaactaag gtgttaccat taacatcctc
     1021 taaaacctta ttttctactg atacagattg cgaatatcca atttctgtaa actaaaaacg
     1081 tgtgataaat acaaataaac aaatgaagca gtttataatt ttattcaaaa tgcagttctc
     1141 cttgtagcaa cgtaaatatt atttactata tttttaactt ctcattactt attaatctgg
     1201 taatagctag aaattataat ttttataggg gttgttatca tcgttatagt aataagcaaa
     1261 attatctcca attacttttg aaacttctct ttgttttaat gcaatctttc tattagttac
     1321 aatagtagga tacaggaaaa aaacaccaat aattaacgac gtatttacaa ccatttcact
     1381 agatggaata atatcaaaag cgaccacaag attatatcca aatcctaaag acgcgtaaaa
     1441 agcatctcta agaaaaatct gttttcctac taccttatcc ctatgaaata aattgttttt
     1501 atcataattc atcttcaaga aaataacgaa tatacaaata gaaattaaac tataaaactt
     1561 attcatatta gtaatataaa caacgttcat tccacctact acaactaaca tgaaagatcc
     1621 taacccaagt gtagcagctc ttccgtaatt cttaatattc catttttctt tctcagttat
     1681 agttttaatt tcattgctta ttttaggaac ataatttttt gctactttat acattttgta
     1741 ttctagaatt atgtttccaa taaataaaat agctagaatt acaaatctca taatatattc
     1801 cattgaacca atttcaaatt tataattgta aaaagccacc atacatgaaa taacttttaa
     1861 agcaaaatca actctggctt ctttctttac tatagaataa tcacttttgt aatcatttgc
     1921 gagtataaaa gaagctattg ttaaaaataa aaaaggagaa atagcccata atgcactaac
     1981 acctattaaa gttttatatc cattaatata ggcaaatgca aaattattta tatatataat
     2041 gattgtgatt aatattaaaa attctatacc cttcttcatt ccaataactc acctcatatt
     2101 gtgattctac gatttaaaaa cataataaaa ccctctcaaa ctaataatta atagactact
     2161 atatactatc taataattaa gctacagcga aagttagacc tgtagggaaa cctactccaa
     2221 tatccatacc tttaccacta gaaatcgctc gagtagcagt tattgcaaaa tttgttgcat
     2281 ttgctgctaa tacagctcca gttacccaac ctaatgctgg aatagtagat gccattgctg
     2341 ctgcaatacc ataaatactt ccttcaattg ctacaactga aactccagta gttgcaccta
     2401 ctccagaaaa gacaatccct tttaatgtac tattgctgat gtagtaacca ccatctacat
     2461 actccatctc ttcattttca atctctacat agtttgaagg taaaactaaa ttactcattt
     2521 atctatcact ctctttcaat tttttataca cacatttgta atattgtgtg tataaatata
     2581 taatttaatt ggttaatttc tcttagcaag taattattaa ttgagagagc ttcattacgt
     2641 ttttaaaatt gtagttgcag ttcgatcact tcttacaagc taatgccaat aaaatataat
     2701 tttttatata tagtttaata ttttcccctt tggttcctcc ctgataataa attctctttt
     2761 tttctctaac aattcacccc attctgattc tgcaatttaa aaacattata aaccctctca
     2821 aatcgataat tagaatacta attcatgctt atttaacagc gaaagtcaga cctgtaggga
     2881 aacctactcc aacgtcaata ccttttcctc ttgaaattgc ttgagttgct gctagggcaa
     2941 agtttgttgc atttgctgct aatacggctc cagttaccca acctaatgct ggaacagcag
     3001 acgccattgc tgctgcaata ccataaatac ccgcttcaat tgctacaact gaaactccag
     3061 tagttgcacc tactccagaa aagacaatcc cttttaatgt actattgctg atgtagtaac
     3121 caccatctac atactccatc tcttcatttt caatctctac atagtttgaa ggtaaaacta
     3181 aattactcat ttatctatca ctctctttca attttttaat attcacattt gtaatgttat
     3241 gcgtaaaagt atataattaa attggttaac ttcttttagc aaataattat taattgagag
     3301 agcttaatta tgtttttaaa atgtagttat agttggatct aatcatacaa acccacaaaa
     3361 tataaatttt atattttgtg ggtttgtaag agaacaatca ctcattttat gccaatttgt
     3421 tctaatgcat aatctaaata tgtaattttt tcatacttaa ttctaacttc agtcaacatc
     3481 cctaatgata aatttactat attatcatta ctatctttca aaaatttttg cttgggttta
     3541 atatgaactt taaaatatat attgttagat tccttctttt catcaagtat tgcatcttct
     3601 gcaatactaa ctatctcccc ttcaacaact ccatactcac tttgacttaa acctgccact
     3661 accatctcaa catcatcttt aatttccacc ttagctcttt cagttgatgg taaatttaac
     3721 tcaattataa aattttcatt ctcttctgca atactcccta ttaaggcacc tgcttgaata
     3781 accattccca tagtgatctt attacttaaa tgaaatttac catcttctgt agcaaaaatt
     3841 ttatattctt ctagactctt tactaaagcg tttttttgtg attctaattt atttatttca
     3901 atctcaattt gttttttttc attggtaaat tctaagagta cttcattctt ttcttgttcc
     3961 attttattaa ttgctttatt cttataatct ttaatctgtt cttcattata gttttgttct
     4021 ttaagggaat cttcggtaac ttttttaact tcaaattctt tcatttttac ttgtaaagaa
     4081 tttaacttat tataaaattc aagctcccta ctcttttttt tatcaaaatt atttgtccct
     4141 cgtgatacgt tcttttgggc tctttctaat aattctattc gctcattcaa atgttttaat
     4201 tggccatcta attgctcaat ttgcaaatta gactgtacag gttttaatga taagagtaac
     4261 tctccttttt taactctctt tccttcttct acatgtattt cctcaacagc tcccgataca
     4321 ggcgacataa tattatgttt attttgcgct gtgacaattc cttgtccttt aacaacataa
     4381 gtcttaattg aatacgttga ccacgttata atagttatta ataagataat aactattgaa
     4441 attatatata acataaacct tggcggattt ttatcgtata ttatacgact atccgtaatt
     4501 tcgtttagat tgtatatttt caaccttatc tcctacccca ttaatcttgt aatggcatga
     4561 aaatatccgc catcaattta tcgctttttt gtttcatata gactgtgtaa ctttcaccct
     4621 gtaaattaag attgtgctca aatgcatgta cgttcagttt tagatatgca aattttatat
     4681 tttcttcttc atcattaaat cttgcaaata aacaattctc tactattaat tgctttttaa
     4741 actcataatt cacgtccgaa atatggtgtg ggctatctaa ttgtacaatt aaattcatat
     4801 taaaatgaat attgccctcg ctatcgactg atgaataaga atgactaatt aatggaccca
     4861 cgatggactt cccatgtgat ttcacataac tttgcatcat atgtgatgtt ttctgaattt
     4921 ctaatggctc attctttttc actttaccaa tcaatacgtt gttaagtttt aaagttttct
     4981 ttacggtaat tgaaattttt tcaactgtac tcaattatct catcccctat atactttttt
     5041 gtatcctctt caatttgagc ttccaaattt tctacggacc cttcctcagt tacagagcct
     5101 cctacttgac taacatataa gttataatac gtacctttat tctccatcat agtttgatgt
     5161 gtcccttctt caactatctc tccttgatct agaacgtaaa tattgtcaca attttgaata
     5221 gttgaaagcc tatgagcgat tattaacatc gtagaatctt ttttcttctt ggagaataac
     5281 gtattgtata cattagcttc actaataaaa tctagattac ttgtagcttc atctaaaatt
     5341 gtaaatttat tatttaatat caatgcacgt gctaaagcaa tcttttgctt ttccccacca
     5401 gaaagtcctc caccagcttc atctaaatag gtatcatact ttcctggtaa cttctcaata
     5461 aattttttac acccagcatc ctcacatgcc tttttcagct gttcatcagt gactcctgtt
     5521 atacccaaag agacattctc cctaattgtt cctgagaata actctatatt ttgtggaata
     5581 tatgaaatgt ttcttctata gctatataaa tctaaatcat caatgtcggt ttccgaaatc
     5641 tttatattcc cttcctccgg agaaaagtac cttaatatca atttagtaat ggtagtttta
     5701 ccactacccg aaccacctac taatgctacc cgctttccct ctggaatagt aatggataca
     5761 ttctttagta ccggacttct actaccatat ctatacgtta tattttctag cctaatatca
     5821 ccagataatc cctctacttt tttctttttg tcattcactt gttctttttc tacatcatac
     5881 aattctgata ttcttgcaag agatacactt gcttcttgaa tagtaagctg aatatgtatt
     5941 aatctaccaa taggatccat aaaataacct gataacgtta caaaagccat taacccacct
     6001 aaagttatat ctttatccat aaccatactc gtgcctatgc acattattaa aatttgccca
     6061 ataccagaaa cactagaaga aaaagtattt tgaatattag atagcacacc ttctttaaaa
     6121 ctaattctta aatttttcac atactcaatt tctaattttt ctaatgtttt ttcttccgct
     6181 gcatgaattt taatagtttc tatacccttt aatgtttcta caatatgact gttaagaata
     6241 gagtccgatt ctttcttacg aaaatttatt tttttgtacg gccctttaaa gataaaaact
     6301 aaaattgtat tcaccaaagt caaaagcaag accacaccga aaagtgtatt gttcataaag
     6361 taaagaacta ctgcagatgc tatcgctaaa aataagtcaa taaataaaga taaggttaca
     6421 gatgttagta tgttcttaat tgtaaatgca tcactaaatc tcgttaaaat atctccaact
     6481 ttacgcgtag aaaagaattc catcggaagt ttgtatgtgt gtctaaaaaa tcctaataat
     6541 agctgaatat caattttttg tgataaatat agcaatacat gctgtctaat ggtagatatt
     6601 gcaacctgaa ttataactac aaataaaaat cctaatccaa atgcagtcag ctcatttttc
     6661 agattataag gaagtatttc atccattaat actttattaa agaaagccga tgaaactcct
     6721 aaaattgtca gtagtaaaga tgcaataata ctgtaaacaa ataacatttt ttgtggtata
     6781 agtaatttaa taaaagtata caaagtgttt ttattttttg tactttccac aacaaactca
     6841 ttgtttggag ccagcaataa taaaatccca tcaaaggaac taaaaaactc aacaattgat
     6901 attttctgtt ttccttttgc aggatctaaa atcacaacaa aatgttttgt gattttatgc
     6961 acaacaacaa aatgagttag accttcttga gtaataacat gagcaattgc cgggagagta
     7021 aatgtactag taaacgtttc acgatttacc ctaaccgctt ttgcatcaaa acctaatttt
     7081 tttgcccctg attcaaggcc acttaatgtc gttcctttaa tatcagttcc taatatatcc
     7141 cttaactttg taatagatat atctttctta tagtacatac atactgtcgc aagacaggca
     7201 gctgcacagt cggtcgcatc atgttgttta actaaatatg aaaacattct aacttaactt
     7261 tctcctctca cattctaatt aacccttttt atgactaata atgtcacagt cacaaattct
     7321 taagtagtac catatccaca aatatcttcc taataagaaa taatcttatt aagagtgctt
     7381 ccatacaaac caaatatact atgtagaata ttaccaaaag cgcatctgat tttatcgata
     7441 ttttatataa cctctaaaca tggattttga atatcgatag ggatataaat catttatcat
     7501 tgcatacgga cttccgttcc aatatctcat taacggtgtc catagcctat ttttttttat
     7561 ccatttaact gtgtaatact taaataattg atcatccgtc aatttttcct tttcctcgat
     7621 agtccatctc agtgcctcta gtgctctttc tcttgtccaa aagcgagttg gtgtcatttt
     7681 aaactcccat tctttgaact gattaggata tagatcgttt atcattgcat atggactatc
     7741 attccaaaac aattggcaag ccccttttaa ccccgatttc actagccact tgtgaccata
     7801 cattagcctt aattgttctg gggtcaatac ctcttttttc tcaatggtcc atttcaaggc
     7861 cttaagtgct ttttcttttg tccaaaattt attgggaacc atcgaaaatt gccattcttt
     7921 aaaaatatcc ggatataaat catttatcat tgcatatgga cttccattcc aaattgttct
     7981 taacggggca cccagtctct gttctttcaa ccatttttgt ccatatactt tcaaaaggtc
     8041 ttcaaaggtt aattttattt ttacttcaat ggtccatttt aatatttgta atgccttttc
     8101 ttttgtccaa aaattcaacg gtgtcatccc taactcccac tctttatatt gttttggata
     8161 agcgtcatcg agcattttat acggactatt ttcatattta aactgtaaca tccctctcaa
     8221 gcgatacttt acaagtaaag atgtattcca gttctgttta atctcttttt tattccattt
     8281 caaaattttt tcaaccaagt gttttgttac tcttttagcc atttcattat tattatctaa
     8341 attccaagta ccaggtggaa atctacttct tttcccatca agtatttctt gataaatttc
     8401 ttcaatagta attatctgta cttgcatttt aaaatctcct tttaatactc acacaccatt
     8461 tatgtacact atatacttat aatatttatt ctctaactta gagattctat tacataaact
     8521 aattaacgtc agaaaaagga tttccattga taataaatta gccaagaatt cattttcatc
     8581 tttttcaatt agagatttac taacagtcta tttcttttta tcattactct atgactgtta
     8641 gataaagtta actcttgata tgaggttatg aaacacaaaa aattaaatac cgtaaaagaa
     8701 cggtcacata tttgtatttt ctttcttgct ttacttcaat tatcaaatgc aacaagtttc
     8761 ttttacaccc tatcacgatg tattaagtta tttagtgaca tgaatatatt aaatcaaaaa
     8821 tataacccta taatgtataa aatttcatga tactaatgaa gttttataca ttatagcttt
     8881 ttgattagta acattaacgt aaattaaaaa ggcatgaatt acgtataaca cgttttcatg
     8941 cctttctata aactgtctaa tagaataatc tagccatact cctagagaaa ttcttattct
     9001 tataattaat tttaatgttt acgaagtttt acttaataaa tcatatactc catccacaaa
     9061 catacaactc caaataaagt ggtcgtatat gtaaataccc tccctgctct tggaagcgtt
     9121 gagtctgcgt cttttatttt acaaattttg tgatactgga aagtccatat cgtactttag
     9181 tccgccttta tgcatgggat gaaagaatcg ctttttcata gaaaaacagt tgtgttttag
     9241 ttatatgaaa actatagaaa agtagaagtc tttaatacga cattgtgtcc tcgaaaacaa
     9301 gatatcatcg agagttcggt agccgaatgc atgctatcgg taaaatgaga ggtgtcgtca
     9361 agaacaagac gctactacac ctgaacacga atgtatatgg tctgtcgtga gagtggtgat
     9421 ggcacaccac aatcttgcac gtcagtgata ccagaaacgg tcatctctgg ataacttata
     9481 aaatgagaac tatactagag cctagattta tcatcagttc ctgagtaaga accctttact
     9541 ccctgatatt taatgtaaaa tgaatgggga ttttgttttc cagatttttc atacgcactc
     9601 tctttaccac ttttaattaa agattccttt tcaggttcga atgtaaatcc gcctctacca
     9661 cttgtatttt ccaaggttat ggacacatag ctattttgta tctatctcag gaaatgtatc
     9721 gagtgcgact attaaacgat atgtggacag ccatatttct tatatttaaa ttcttccaag
     9781 tttgtattga gtattgtacc tttttagcta tgcagaacca cctacaaata atagtatccc
     9841 tagatctaac accattattt gtaggggata atatcattat tcaagaattt ttttaatata
     9901 cttatactaa tttttaaata acacctttta aaaccatacc taaaactgcc atgattactg
     9961 aactaataac aattcttaat atccatgttg tattcaaatt aattttttct aattgcttat
    10021 taagcatagc aatatctttt tcgctaatag tcgtacgagt ttctaaatta cgaatgtcct
    10081 gcataatctc tttttgccct gtttttaggt cgacaatttt cgcgtataga tcctccattt
    10141 tttcacatcc ttttttcatt ctaattgtag aatctttata tattatgaga caaccccatt
    10201 caaagtgaat atatatttaa gcttgtacat gtttaacttg cacaagcttg aaatatatgg
    10261 ggtaattttt ctatacccta attatgataa tttattttct gaactgaaat ttaccgtgat
    10321 tttctaatat ttaatccttc aaaaataaat ttgaccaatc ataacaaaag cactccctaa
    10381 ggaatgcttt tgttttaatt cattttttca tttgaagacc gactgtttcc gcacccctct
    10441 tcaattggac ctaaactaaa tcaactttgc aaaaaagata tttccattgc tcgtaaagcg
    10501 aagttcatcg aggcagtcct acaagaaatg aacatcggtg aactgtctgc agatatgaat
    10561 aaattcattc atgttttaaa gcatacttgt catcgtcaaa ttcgcagtgt aattcgtggt
    10621 cttcgtgaca tggttgatcg aaaagaagga tatccaacga aaattgtata tacattgaaa
    10681 aaactattgc atcaaacatc tcaatatcaa attcttgata cagcggcgaa agaaggcatc
    10741 taccctctta ttgcccagca tataccaaaa gagagaaata gtgaccgtga acaagctgtc
    10801 tttaattttg gtctacatta ttccatgtac tctctaggca acattaagaa gatgtttaaa
    10861 aatgtgcatg cactactgaa gcagaagttt gcggtaccag tgacggaaga gtcttatcac
    10921 cgtaattatc ttaagtacca ggaagaaacg ttatttagaa aatatgcata tgatcaggga
    10981 gtaaatctcc atgcatatat cgctttagaa attgaaatgc gtgaaaaact aaaagttcgt
    11041 ggtcataaag aacacacgat tccaagtgat gtgcgtgaat ggtttatcga ggctattgat
    11101 aagctgccac aagaaaaact tcgtgttatt aagctaccaa agcaatttaa tttactagag
    11161 tttatgcgta cctttgaacg tttagtacgt gctggtgtaa caataacagc ttcggatgag
    11221 gtgctaaccg caatggaaat acaaaaatag caactagctc tctatttttg aaaaaaccaa
    11281 aagtagaggg ctattcgtgt ttgtttcatt aacccccctt tcccactact ctatttatac
    11341 atatcaaatc agaaggactt catgaaaaat ggggtctttt tttatgcaat tctatacaaa
    11401 tctgtttcta actatacaag aatctccaca ctacacattg ggtatttttc acgtgtagta
    11461 aaatatccga ttttatatct acaaaaaatc agaaggatat ttataaaaat agtggatatg
    11521 aggacaatta taatcaaatg cgaacaatat tttccaagaa ttgttgataa tactagtgtc
    11581 gttcatttca tctaataaaa atcatcaata ataatgtgaa ttatgtttaa atactgagta
    11641 atgttaacaa ttagaaactt aatctgaaga cattgcggat aatcgaacgc tagatacttc
    11701 tatatttacc aaaataaaga aaatgcatcc cgttttcctg ctcggataaa tataaagtga
    11761 tttgtatcaa tttctttccg atttcatgta gaaatatatg attttctttc tgcttttttg
    11821 tagaattgaa agtctaataa tccttgaatt tgcgaagaca tattcttcaa tttatgattt
    11881 tttatgttct tatatcaact cctcccttgt gagagtaagc ctatacccta tttttgataa
    11941 cctataaaag ttttatcttt atatttatta atatataaga tatatattaa taatagattt
    12001 tattattaat actcaagata ttaaataaga ataaacatct taagaacaca acaaagaaca
    12061 atatataata agaaaatgcc aatatacatt tgacaccctt attaaatcta caattttaat
    12121 tttagattca cccacctaaa attaaaatag ttttacagac acaacaacca ctcttaattg
    12181 tgcttcgtca ctatcatcca atggctgaaa tgttattctt atggttatga tgtactagaa
    12241 ggtacac
//
