LOCUS       NZ_MOOL01000058        19486 bp    DNA     linear   CON 03-APR-2024
DEFINITION  Bacillus thuringiensis serovar xiaguangiensis strain BGSC 4BN1
            contig000058, whole genome shotgun sequence.
ACCESSION   NZ_MOOL01000058
VERSION     NZ_MOOL01000058.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis serovar xiaguangiensis
  ORGANISM  Bacillus thuringiensis serovar xiaguangiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 19486)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Comparative genomics of Bacillus thuringiensis reveals a path to
            pathogens against multiple invertebrate hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19486)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, 1st Shizishan,
            Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.5.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002147905.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 02:08:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,596
            CDSs (total)                      :: 6,434
            Genes (coding)                    :: 6,198
            CDSs (with protein)               :: 6,198
            Genes (RNA)                       :: 162
            rRNAs                             :: 15, 15, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 1 (5S, 23S)
            partial rRNAs                     :: 15, 12 (16S, 23S)
            tRNAs                             :: 114
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 95 of 236
            Pseudo Genes (incomplete)         :: 153 of 236
            Pseudo Genes (internal stop)      :: 70 of 236
            Pseudo Genes (multiple problems)  :: 63 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:39:27
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 19486
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOOL01000058.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..19486
                     /country="China"
                     /db_xref="taxon:180870"
                     /isolation_source="bean"
                     /mol_type="genomic DNA"
                     /organism="Bacillus thuringiensis serovar xiaguangiensis"
                     /serotype="H51"
                     /serovar="xiaguangiensis"
                     /strain="BGSC 4BN1"
                     /submitter_seqid="contig000058"
     region          1..19486
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX) and not alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..19486
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..19486
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[17476:>19486](-)"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(17477..>19486)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     gene            39..593
                     /locus_tag="BK733_RS24045"
                     /old_locus_tag="BK733_22960"
     CDS             39..593
                     /EC_number="2.-.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006097282.1"
                     /locus_tag="BK733_RS24045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_22960"
                     /product="GNAT family protein"
                     /protein_id="WP_063546646.1"
                     /transl_table=11
                     /translation="MEDTYKIPKLETKRLLLKKLDFHDLDDLFEVYSDPQTTTYVPREV
                     HKNKDETRIFLENTMDTTKKGKSFIWSIMFKGDQKAIGTCGIWKLSHSSASLGAVINPL
                     YWGKGVIVEALEELIKFGFQELDLNRIEGRCDIRNTASERVMQKLKMTYEGTLRQSVMI
                     NGMYCDSKVYSLLKHEYGNFR"
     misc_feature    complement(82..95)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.27"
                     /tool="antismash"
     gene            731..2115
                     /locus_tag="BK733_RS24050"
                     /pseudo=""
     CDS             731..2115
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028482.1"
                     /locus_tag="BK733_RS24050"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="IS4 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSNSVSDDLQLFAQEIKSLLSSNTLQNLARDVGFVQRTSKYQAQD
                     LVALCVWLSQSVAKTSLTQLCSCLEASTEVLISPEGLNQRFNAAAVQFLQQVLAKLLNQ
                     KLFSPRLFSSSYNSIFKRIRILDSTAFQLPDVFSSAYPGAGGCSHKAGVKIQLEYDLLS
                     GQFLHIHT"
     gene            complement(2816..4657)
                     /locus_tag="BK733_RS24055"
                     /old_locus_tag="BK733_22980"
     CDS             complement(2816..4657)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312689.1"
                     /locus_tag="BK733_RS24055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_22980"
                     /product="serine hydrolase"
                     /protein_id="WP_088021126.1"
                     /transl_table=11
                     /translation="MAYAWQARHGLTSTQFQVEFNSLLSQGYRLIDISGYGVSGVDRYS
                     AIWEQSPGPEWQARHGLSSGDHQALFTNLAAQGYRPVRVSGFEVSGTDRYASLWEKTAG
                     PEWRARHGLTAAQYQSEFNNLISQGFRLIDICGYNVAGEPRFAAVWDKSPIPNWVARHN
                     LSSADYQTEFNHWSNQGFILWRVSGYEIAGQNFYAAIWIKGPALTWSARHGLSAADYQA
                     EFNSMLSRGFRLVRVTGYPLHGEVRYAAIWHKPYLSDSDLAFIQQTVTTFMNNHGIPGA
                     SLALTYQERLVFAQGYGLANRATNELVTTNHRFRIASVSKPITSAAVFKLIEDGRLKLG
                     DRVFGTNAILGTTYGTQPYSTNIDQIMVQHLLEHTSGWAQTQDPMFSHFNLNQDELITW
                     MLDNERLTYVPGRTFDYLNFGYLLLGRVIERVSGMAYADYVRQHVLAPCGITDMHIAGD
                     TLADRRANEVVYYPQSSSNPYTIRVSRMDAHGGWIASATDLVRFLVRVDRFSSKPDILN
                     RGSLATMYTTTTAPKIDGTPSNYAKGWAVHPSGNYFHDGDIPGSASIAVRTHHGYCWAV
                     LVNSRNDVQLTKMRSDLDNLMWTIITRIRDWPAYDLF"
     misc_feature    5307..5324
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.23"
                     /tool="antismash"
     misc_feature    complement(5349..5363)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 20.05"
                     /tool="antismash"
     gene            complement(5488..5640)
                     /locus_tag="BK733_RS34875"
     CDS             complement(5488..5640)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812117.1"
                     /locus_tag="BK733_RS34875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_176328732.1"
                     /transl_table=11
                     /translation="MKQVEEKRIMIYVVGGNVKVLRKPHDVVLDIVDFDNLEEYVEPTF
                     TGLLY"
     gene            complement(5807..6880)
                     /locus_tag="BK733_RS24060"
                     /old_locus_tag="BK733_22985"
     CDS             complement(5807..6880)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124577.1"
                     /locus_tag="BK733_RS24060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_22985"
                     /product="patatin-like phospholipase family protein"
                     /protein_id="WP_063549436.1"
                     /transl_table=11
                     /translation="MTYPFRNLVFEGGGVKGVAFIGAIRALNDEEILPKIQRFGGTSAG
                     AITALLLGLSIPFADIVKIHKDMNFKAFKDHDFLFIQDNIRLFFDGFGIYKGDFFTEWI
                     GAIIEKWTGSKDTTFQQLYDKTGKDVFFQGTNVSTHQLRTFSRETTPDVPLVVAVRISM
                     SIPFFFKSVEWEEDYYVDGGVLDNYPIRLFDRKSFVSKEEHFTTTDTIDEVNKLVSNTD
                     QHSALRNLHTNYNVSFFDPADEIVYNKETLGFRLESRAEIAMLKHIASAKKHHIGHSLI
                     DFVWNLSQTMIKGQASGKTTKEDAARTVFINTFFTSSINFDITEKEKKTLIDSGYTCTK
                     DYLHQFNTSEVKLFNRP"
     misc_feature    complement(6841..6850)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 17.24"
                     /tool="antismash"
     misc_feature    7102..7117
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 20.83"
                     /tool="antismash"
     misc_feature    7102..7118
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 23.21"
                     /tool="antismash"
     gene            complement(7763..8881)
                     /gene="tnpB"
                     /locus_tag="BK733_RS24070"
                     /old_locus_tag="BK733_22990"
     CDS             complement(7763..8881)
                     /codon_start=1
                     /gene="tnpB"
                     /gene_functions="other (smcogs) SMCOG1192: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981148.1"
                     /locus_tag="BK733_RS24070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_22990"
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_176328762.1"
                     /transl_table=11
                     /translation="MMNKAYKFRIYPNQAQAILINKTIGCSRFVFNYFLSLWDQAYKET
                     GKGLTYGTCSAKLPAMKKELVWLKEVDSIAIQSSVRNLADAYTRFFKKQNSAPRFKSKK
                     NNVQSYTTKQTNENIAVVGNKIKLPKLGLVRFAKSREVEGRIVNATVRRNPSGRYFVSL
                     LVETEVQELPKTSSYIGMDVGLKNFAILSDGTTYKNPEFFRSLEEKMAKAQRVLSRRMK
                     GSSRWNKQRVKVARIHEYISNARKDYLDKISTEIIKNHDVIGIEDLQVSNMLKNHKLAK
                     AISEVSWSQFRNMLEYKAKWYGKQIIVVAKTFASSQLCSCCGYQNKDVKNLNLREWDCP
                     FCRTHHDRDINASINLKNEAIRLLTARTAGLA"
     misc_feature    complement(8922..8937)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 22.12"
                     /tool="antismash"
     gene            complement(9178..10164)
                     /locus_tag="BK733_RS24075"
                     /old_locus_tag="BK733_22995"
     CDS             complement(9178..10164)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913371.1"
                     /locus_tag="BK733_RS24075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_22995"
                     /product="DUF4046 domain-containing protein"
                     /protein_id="WP_088021127.1"
                     /transl_table=11
                     /translation="MLKKEVSIEDIYQEILDGKRIRFPPNTWKEDIDNKMARRVLTYLL
                     NSILKWNKEDIRKKWNTKLLVKYRLRGLLKHRYENSPFKAINDLYPNQFKEWEFGMTPL
                     NFWTKEKALTILKWIIEEKEGPSQEKLLGLYGKKWLKKNKLSAPLAMYWNSSPYAMIND
                     LYPGRFKEWEFGMTPNNFWTKEKALEALKWTIEEKEGLTPKQLLDIYNIKWLKTHGLAS
                     ACQIIWGNSPFRMINDLYRDRFKEWEFRVTPVGFWSKRKALEALRWTIEEKEKLDEKQL
                     LKVFNQKWLIKQKLWTPLKCYWKGSPYEMLIALYPNRFSKNMLKGYI"
     misc_feature    complement(10279..10292)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.28"
                     /tool="antismash"
     misc_feature    10279..10293
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.7"
                     /tool="antismash"
     gene            10391..11884
                     /locus_tag="BK733_RS24080"
                     /old_locus_tag="BK733_23000"
     CDS             10391..11884
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413415.1"
                     /locus_tag="BK733_RS24080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_23000"
                     /product="peptide MFS transporter"
                     /protein_id="WP_088021128.1"
                     /transl_table=11
                     /translation="MEKISKSDIIRTVPQSGFFGHPKGLFTLFFTEFWERFSYYGMRAI
                     LLYYMYYSISQGGLGMDHATAASIMAIYGSLVYMSGIIGGWIADRLLGSSRTIFWGGVL
                     IMIGHLILALPGTISTLFISMVFLVLGTGLLKPNVSSIVGDLYSETDTRRDSGFSIFYM
                     GINLGALLSPFVIGTLGQKYNFHLGFGVAAIGMLLGLIIFVLTKKKNLGLAGTQVPNPL
                     TDIERKKVIIQFSIGTIAIVLFCILTIPTGILTINRFTLLVSILGILIPTGYFILMYRS
                     PKTTKIEKSRLIAYIPLFIAATMFWAIQEQGAIILASYADQRTQLKLAGFELQSSWFQS
                     LNPIYIVLLAPVFAWLWVKLNSRQPSTANKFAIGLLFAGLSFLVMIIPAYIHGTESLVS
                     PMWLAISFLLVVIGELCLSPVGLSITTKLAPAAFSAQTMSLWFLTSAAAQAINAQIVRL
                     YNPETEIIYFGVIGGLSIILSVILYFMAPKIQVLMKGIK"
     gene            complement(<12014..>12097)
                     /locus_tag="BK733_RS34305"
                     /pseudo=""
     CDS             complement(<12014..12097)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054743.1"
                     /locus_tag="BK733_RS34305"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MIVSSRLIFSLSKVNKRIEYTVEDVQSY"
     gene            12117..12425
                     /locus_tag="BK733_RS24085"
                     /old_locus_tag="BK733_23005"
     CDS             12117..12425
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BK733_RS24085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BK733_23005"
                     /product="hypothetical protein"
                     /protein_id="WP_088021129.1"
                     /transl_table=11
                     /translation="MRECQNHQDHQVEAEVLLALCDAIPSSLALGNALNTAVENFHLQL
                     NYNLSPTDTVILVVKSVNLQTFTNQGTELATTNNITVSCDFAFANFIDLQVTSEPTL"
     gene            complement(12534..13259)
                     /locus_tag="BK733_RS24090"
                     /old_locus_tag="BK733_23010"
     CDS             complement(12534..13259)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016083260.1"
                     /locus_tag="BK733_RS24090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_23010"
                     /product="hypothetical protein"
                     /protein_id="WP_064532202.1"
                     /transl_table=11
                     /translation="MVYEIKFIPYEELIFFEEYNEEQLIKIKSNIDAQKALSNLPIVMR
                     IESNKYLVLDGTHRILALNKLRCMNITAQIIDESIVVCSSWLHQIPKCEELISDLKNNP
                     NFYFTGENLLEQKYLARVYIDNQVYYLYDMNSKKKEEAISFLHSIINVYDSKYGYKKIT
                     KEEYTPNLEYITIEFPDFSIQEIMKSIDNKLLPLNVIRFTFNIKNMNGINIPINVIRSP
                     EAYEFEWREIIEKLSLPSH"
     gene            complement(13423..14442)
                     /gene="sbnB"
                     /locus_tag="BK733_RS24095"
                     /old_locus_tag="BK733_23015"
     CDS             complement(13423..14442)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="sbnB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1158: ornithine cyclodeaminase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016096263.1"
                     /locus_tag="BK733_RS24095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_23015"
                     /product="2,3-diaminopropionate biosynthesis protein SbnB"
                     /protein_id="WP_063549469.1"
                     /transl_table=11
                     /translation="MLLETKPELIYLSKQNIIDLGGYKANIYVQAITQALTLHSERNFS
                     QPLKPYLKTNKEGEHIADRIIAMPAYLGEPSISGIKWIGSKFDNPTKKNMERASALIIL
                     NDPETNFPIAILEGSVISSMRTAAVTVVAAKYLAKKGFQNVSIIGCGLISKMHINSLLE
                     QFNHIRQINLFDLDNVKAKAFAQEMKERYSHVDFQVKESAKNAVEQAEVLVTCTVADTP
                     YIEADWIQKGTFVSNISIMDIEKEAFLKADKVIVDDWDQANREKKVINQLVVEGKFSKE
                     MLHAELGDIITGKKLGRVTDEEIIILNPMGMAIEDIACAHAIYEKAKQEGLGKVLSLY"
     gene            complement(14477..15460)
                     /gene="sbnA"
                     /locus_tag="BK733_RS24100"
                     /old_locus_tag="BK733_23020"
     CDS             complement(14477..15460)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="sbnA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580988.1"
                     /locus_tag="BK733_RS24100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_23020"
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="WP_000580988.1"
                     /sec_met_domain="PALP (E-value: 2.7e-68, bitscore: 220.5,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIHDDVLSLIGDTPIVKINKLVPPESAQLYVKLEGFNPGGSSKDR
                     VAKRVIEYAENSGQLKPGGTIVESSSGNLAIGLALVAKMKGYKMICVVDPKISKVNLNL
                     IKSYGAQVHMVDVSDEHGNYLKARLVIAQDLANSIDGAYWPNQYNNPENPEAYVHTLVQ
                     EIYDDFNDTLDWIVCPVGTAGLITGVAKGMKKLNPSVKVMAVDAVGSVIFGGKPGVRRL
                     LGIGNAIVPGNVNRSLYDDLSYVNDADAFFITRELVSKEGILVGGSSGAVVCAALRLID
                     KLPKSKKVLAILPDRGDRYYNTIFSDEWLVEHDIELPQSINDSILV"
     misc_feature    15496..15509
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.23"
                     /tool="antismash"
     gene            complement(15539..16300)
                     /locus_tag="BK733_RS24105"
                     /old_locus_tag="BK733_23025"
     CDS             complement(15539..16300)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930877.1"
                     /locus_tag="BK733_RS24105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_23025"
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="WP_000930877.1"
                     /transl_table=11
                     /translation="MLNDLKVIPTEQIYLHEEYEHERLKKLSHKIKNEQVLKNPPIALQ
                     LAEDKYLILDGAHRTLSLKQLNCKRMVVQVVKADYLSIGAWSHHISDSEGVLEVLKSNS
                     NIFLSENIESENLIATVFVNGIKYDLAINPEMEKDMEIKIRIWKEIVNAYTNKYPFTRI
                     TNDELIPSDGIVFSYTLLELYQIKQLVESNLLLPAGVTKFKLNCGRILNLNIPLSFLIR
                     EDFVEEDWNELLELWGKSIRLYTDPVLLCEV"
     gene            complement(16692..17456)
                     /locus_tag="BK733_RS24115"
                     /old_locus_tag="BK733_23035"
     CDS             complement(16692..17456)
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Thioesterase (24-246). E-value: 8.3e-52.
                     Score: 168.8. Matches aSDomain:
                     nrpspksdomains_BK733_RS24115_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thioesterase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1004: thioesterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060110.1"
                     /locus_tag="BK733_RS24115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_23035"
                     /product="thioesterase domain-containing protein"
                     /protein_id="WP_088021131.1"
                     /sec_met_domain="Thioesterase (E-value: 2.4e-50, bitscore:
                     161.9, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNIYSPSLYFKKYNKVNSFPTSMRLFCFHYAGGSASNFKKWATK
                     LPEQVEVIAVQLPGRENRIMEKPFNNIQSIICELAVEILPLLDKPYVFFGHSMGALLSY
                     EMSWYLKENFSTFPEKLIISSFRAPHLSPITNNMHDLSVPIFIEKLKEMGGTSEEILSN
                     QELMELIIPTIRADFEVCETYVYKERNKLNCPIVAYGGTEDSRVRLEHLKQWEKHTSKA
                     FKVQMLPGNHFYLKEVEKELLYLISKELLENK"
     aSDomain        complement(16719..17384)
                     /ASF="active site serine inconclusive"
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS24115_Thioesterase.1"
                     /evalue="8.30E-52"
                     /label="BK733_RS24115_Thioesterase.1"
                     /locus_tag="BK733_RS24115"
                     /protein_end="246"
                     /protein_start="24"
                     /score="168.8"
                     /tool="antismash"
                     /translation="RLFCFHYAGGSASNFKKWATKLPEQVEVIAVQLPGRENRIMEKPF
                     NNIQSIICELAVEILPLLDKPYVFFGHSMGALLSYEMSWYLKENFSTFPEKLIISSFRA
                     PHLSPITNNMHDLSVPIFIEKLKEMGGTSEEILSNQELMELIIPTIRADFEVCETYVYK
                     ERNKLNCPIVAYGGTEDSRVRLEHLKQWEKHTSKAFKVQMLPGNHFYLKEVEKELLYLI
                     "
     CDS_motif       complement(17139..17192)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS24115_0002"
                     /evalue="9.90E-07"
                     /label="NRPS-te1"
                     /locus_tag="BK733_RS24115"
                     /protein_end="106"
                     /protein_start="88"
                     /score="21.3"
                     /tool="antismash"
                     /translation="KPYVFFGHSMGALLSYEM"
     CDS_motif       complement(17355..17381)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS24115_0001"
                     /evalue="1.40E-03"
                     /label="NRPS-beforete1"
                     /locus_tag="BK733_RS24115"
                     /protein_end="34"
                     /protein_start="25"
                     /score="11.6"
                     /tool="antismash"
                     /translation="LFCFHYAGG"
     gene            complement(17477..>19486)
                     /locus_tag="BK733_RS24120"
                     /old_locus_tag="BK733_23040"
     CDS             complement(17477..>19486)
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (311-380). E-value: 4e-22. Score:
                     70.2. Matches aSDomain: nrpspksdomains_BK733_RS24120_PCP.1"
                     /NRPS_PKS="Domain: Thioesterase (398-651). E-value:
                     8.1e-36. Score: 116.5. Matches aSDomain:
                     nrpspksdomains_BK733_RS24120_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thioesterase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406687.1"
                     /locus_tag="BK733_RS24120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_23040"
                     /product="thioesterase domain-containing protein"
                     /protein_id="WP_140158801.1"
                     /sec_met_domain="AMP-binding (E-value: 3e-63, bitscore:
                     203.8, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.5e-16, bitscore:
                     50.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Thioesterase (E-value: 2.7e-33, bitscore:
                     106.1, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="PLCIGETVVLFPEGLDLEELGRWVRKRKYSVIKLTPAHLELLSHQ
                     VSEEDAAFCTDTFVVGGEPLLAKHVDFWVKHAPNTVIINEYGPTEAVVGCTNYIVKPGE
                     NYSKSIPIGKPIFNTEMYVLDTSLQPVPIGVHGELYIGGVGLAKGYYKRPELTAERFIT
                     SPFNSNEERKLYKTGDIVRYLPNGNLEYIERIDQQVKLRGFRIELGEIETILNQYSKIR
                     EGIVLVREDIPGDKRLVGYIIGDDLTADEVEKLSNYMAEQLPDYMIPSAFVLLEKIPLT
                     INGKVDKEALPAPDMSLIKRTEYIEPKTDIEKKLVEIWEELLGYSGISVYDNFFGIGGH
                     SLMAVQLMSKIKKTFNVKIPLTIIFQSGTIAALAKQIDRNKIYKVNNSIVPLQPKGNKR
                     PIFFVHPIGGHIISYFELAKYLGDKRPFYAFQSLGLESEETILTNIKEMANFYIKEMQN
                     VQEEGPYIIGGWSFGGVVAFEMARQLVEKGIKVEAIILLDTWAPDIYNDLSEWDDLAVF
                     MNDLMKQYNLEVSEELILGYKNLSMQDERIRYVISYLKNHSKFKQDIQVEDLEKMIQVF
                     QTNLNAYRSYQPEKLDQDILLFTANHPHKENNHVEDDTLFWGKYVQKELKVIELNATHY
                     SMMQSAHAEDIAYHIDIFLEEISKKLYNSSFNLN"
     aSDomain        complement(17531..18289)
                     /ASF="active site serine present: True"
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS24120_Thioesterase.1"
                     /evalue="8.10E-36"
                     /label="BK733_RS24120_Thioesterase.1"
                     /locus_tag="BK733_RS24120"
                     /protein_end="651"
                     /protein_start="398"
                     /score="116.5"
                     /tool="antismash"
                     /translation="RPIFFVHPIGGHIISYFELAKYLGDKRPFYAFQSLGLESEETILT
                     NIKEMANFYIKEMQNVQEEGPYIIGGWSFGGVVAFEMARQLVEKGIKVEAIILLDTWAP
                     DIYNDLSEWDDLAVFMNDLMKQYNLEVSEELILGYKNLSMQDERIRYVISYLKNHSKFK
                     QDIQVEDLEKMIQVFQTNLNAYRSYQPEKLDQDILLFTANHPHKENNHVEDDTLFWGKY
                     VQKELKVIELNATHYSMMQSAHAEDIAYHID"
     aSModule        complement(17531..18550)
                     /domains="nrpspksdomains_BK733_RS24120_PCP.1"
                     /domains="nrpspksdomains_BK733_RS24120_Thioesterase.1"
                     /final_module
                     /incomplete
                     /locus_tags="BK733_RS24120"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       complement(18032..18106)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS24120_0005"
                     /evalue="1.90E-15"
                     /label="NRPS-te1"
                     /locus_tag="BK733_RS24120"
                     /protein_end="484"
                     /protein_start="459"
                     /score="49.0"
                     /tool="antismash"
                     /translation="QEEGPYIIGGWSFGGVVAFEMARQL"
     CDS_motif       complement(18257..18286)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS24120_0004"
                     /evalue="1.80E-02"
                     /label="NRPS-beforete1"
                     /locus_tag="BK733_RS24120"
                     /protein_end="409"
                     /protein_start="399"
                     /score="8.3"
                     /tool="antismash"
                     /translation="PIFFVHPIGG"
     aSDomain        complement(18344..18550)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS24120_PCP.1"
                     /evalue="4.00E-22"
                     /label="BK733_RS24120_PCP.1"
                     /locus_tag="BK733_RS24120"
                     /protein_end="380"
                     /protein_start="311"
                     /score="70.2"
                     /tool="antismash"
                     /translation="EKKLVEIWEELLGYSGISVYDNFFGIGGHSLMAVQLMSKIKKTFN
                     VKIPLTIIFQSGTIAALAKQIDRN"
     CDS_motif       complement(18848..18913)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS24120_0003"
                     /evalue="1.10E-10"
                     /label="NRPS-A_a8"
                     /locus_tag="BK733_RS24120"
                     /protein_end="212"
                     /protein_start="190"
                     /score="33.6"
                     /tool="antismash"
                     /translation="YIERIDQQVKLRGFRIELGEIE"
     CDS_motif       complement(19001..19090)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS24120_0002"
                     /evalue="4.70E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="BK733_RS24120"
                     /protein_end="161"
                     /protein_start="131"
                     /score="54.0"
                     /tool="antismash"
                     /translation="PIGVHGELYIGGVGLAKGYYKRPELTAERF"
     CDS_motif       complement(19442..19462)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS24120_0001"
                     /evalue="8.30E+01"
                     /label="NRPS-beforete1"
                     /locus_tag="BK733_RS24120"
                     /protein_end="14"
                     /protein_start="7"
                     /score="-2.5"
                     /tool="antismash"
                     /translation="VVLFPEG"
ORIGIN
        1 tatataatat ataaataacg attaactgag gtgagagtat ggaagataca tataaaatcc
       61 caaaattaga aacaaagcgt cttttattaa aaaaactaga ttttcatgat ttagatgatt
      121 tatttgaagt ctattcggat cctcaaacaa ctacatatgt acctcgagag gtacataaaa
      181 ataaagatga aactcgtatt tttttagaaa atacgatgga tacaactaag aaaggtaagt
      241 ctttcatatg gtctattatg tttaaggggg atcagaaagc tattggaacc tgtggcatct
      301 ggaaattatc acacagtagc gcttctttag gagccgttat taacccactg tattggggaa
      361 aaggagttat cgttgaagct ttagaagaac taataaagtt tggctttcaa gaattagatt
      421 taaatcgtat tgaaggaaga tgcgacataa ggaatacagc gtctgaacga gttatgcaaa
      481 agctaaaaat gacatatgaa ggaacattga gacaaagtgt aatgatcaat ggtatgtatt
      541 gcgattctaa ggtatattct cttttaaaac atgaatacgg taactttcga taacgaaaat
      601 tatgtaatgt tatcaaacga ctaaatgagg taccgtcaca tttccataaa gctaaggttt
      661 cacactattc attaagcaaa aaagacgtta ttcttactgt agaattatag gaaaggatgg
      721 cgttttttgt atgtctaatt ctgtatctga cgacttacaa ttatttgcac aggagattaa
      781 gagtctttta tcctcaaata ccttacagaa tctagctaga gatgttggat ttgtacagcg
      841 aacaagtaag tatcaagcac aagatttagt agctttatgc gtatggctta gccaaagtgt
      901 agcaaaaact tccttaaccc agttatgtag ttgtttagaa gcatcaacag aagtactcat
      961 aagtcctgaa ggactaaatc aaagatttaa cgccgcagcc gttcagtttc tacaacaagt
     1021 attagctaaa cttctgaacc aaaaattatt ttcacctagg ctgttttctt cttcatataa
     1081 ttctattttt aagcgtattc gtatcttaga ttccactgca tttcaacttc cagatgtatt
     1141 ttcatcggct tatcctggtg caggaggttg tagccataag gctggtgtga aaattcaact
     1201 tgagtatgat ttgctaagtg gacagttcct acatattcat acatgaccag gtaaaaaaca
     1261 tgatcgaacc tacggttccc tgtgtgtccc aactataaca gcgaatgatt tatgtattcg
     1321 agatttaggt tattttcatt tgaaagatct ttaacatata caagataaaa aggcttatta
     1381 tatctcacgt atcaaatcta atacacgtat ttatcaaaaa aatcctaacc ctgattattt
     1441 tcaagacggc agaattaaga aaggtacaga gtatatccag atagatatgg aggttttaat
     1501 gaactctctt caaccaagac aaacatgtga aatatccaac gcttatgtag gaatgactga
     1561 taaagtccca gctcgtgtga ttgttcatcg actaacaaaa gaacaacaac aaaaacgatt
     1621 acaagatcaa actgtaagag aaaaaagaaa ggaatgcagt attctcctcg tagtaaacga
     1681 cttagcggta tcaatctata tctgtagggg tattctttac gtcggcaaat cgagatttta
     1741 tttaaaacgt ggaaatcatt ctttcatatt catcattgta aaaagataaa acgagaacga
     1801 ttggaatgcc atttgtatgg gcaactgatt gccattctat tctgttcttc taccatgttt
     1861 caaatgcggc aattacttct tatgaaaaag aaacgagaac tgagtgaata taacgccata
     1921 tatatgatta aagattactt tcttctttta caagctatac agaaagacac ccaggagcta
     1981 tcaaagattt taattcgcct gttcaacctc ctacagcaaa acgggcggaa gtctcatcga
     2041 tatgagaaga aaacagtctt tgatatatta ggtgtcgttt acaattgtac catgtctgat
     2101 attcaagcgg cttaattcaa aaaaggaaac ccgttagggt ttatttcgta tgcaaatctt
     2161 taaattccct acacgtagcc tttagaaaaa agaaacatcc tctactataa ttgacgttgt
     2221 atgagcttag cttgatagcg atgtgtgggt accctaatac atcaattaga tgtgtgaacc
     2281 tacccatttg aacggaaatt ggcatagtaa ctatattcat tccctatgaa tcctaccact
     2341 atacggattc gaattgaagc attggattta aaattggaac cagtgactat taaattacta
     2401 ttttgcttgg atactttgat tgtagattgg atagggtgtg ctgatgctgg ttgcgataca
     2461 atgaaggata agtagcttga ggtccacggt gtggaaggac gaagttaaat ccaatggctt
     2521 ggtgactacc gcctgctggg caatttctat tatacggata accgtaataa aaaagtgcgt
     2581 agcaccttcc gcagtttaga tttcttctta agttactttc tttcaacttt gttatttatt
     2641 cttattttac aggaggccct tgcaacggac tgtgtcgatc gtcctcggta ccaatggatc
     2701 cgcagcataa gggctctgta atgatgtttt ttgtcgtgcg ataaccaaga tagaagaatc
     2761 ctttagtatt atgcggtgct aagtcgtgat actctacttt tccttgtcat cgatgtcaaa
     2821 acaaatcata agccggccaa tctctgatgc gcgtaataat cgtccacatc aggttgtcta
     2881 ggtcagatcg catctttgtc aattgtacat catttcgaga gttgactaac acggcccagc
     2941 aatatccgtg atgcgttcgc acagcgattg aagccgaacc tggtatgtct ccatcatgaa
     3001 aataattacc agaaggatga acggcccatc cctttgcgta atttgaaggg gtcccatcta
     3061 tttttggtgc agtcgtggtt gtatacatcg tcgctaggga tcctctattt aggatgtccg
     3121 gtttagagga gaaacgatcc acgcgaacga ggaaacgaac gagatccgtt gcagatgcga
     3181 tccaacctcc atgtgcgtcc atgcgagaaa cccggatcgt gtaaggattt gagctgcttt
     3241 gcgggtaata tacaacttcg ttcgcccgac gatccgcaag agtgtcaccg gcaatatgca
     3301 tatccgtaat accgcagggt gcgaggacat gctgtcgaac atagtcagca taggccatac
     3361 ctgaaactcg ttcaatcact cggccaagca gcaagtaacc gaagttgaga taatcgaagg
     3421 tccgtcccgg cacataagtt aatcgttcgt tatcaagcat ccatgtgata agctcatctt
     3481 gatttaagtt aaaatggcta aacatcggat cctgcgtttg cgcccaacca cttgtatgtt
     3541 ccagaagatg ttgtaccatt atttggtcga tatttgtact gtacggctgt gtcccataag
     3601 tggtgccgag gatagcgttt gtaccaaata ctctgtcgcc aagttttagc cgtccatctt
     3661 cgatcaattt gaacaccgcc gcagaagtaa tcggttttga gacactggca attctaaagc
     3721 ggtggttggt cgtgactagt tcattcgttg cccgattggc aaggccatat ccctgagcaa
     3781 agactaagcg ctcttggtat gtgagagcta aggatgctcc tggaatcccg tgattgttca
     3841 taaaggtcgt cacggtctgc tgaatgaacg ctagatcact atcggacaag taaggcttgt
     3901 gccatattgc tgcatatcgc acttctccat gcaatgggta tccagtaacc ctaactagac
     3961 ggaatcctct agacagcata gaattaaact cggcttgata atcggcggct gataaaccat
     4021 ggcgggcgga ccatgtaaga gccggtcctt tgatccatat cgccgcataa aagttttgac
     4081 cggcaatctc atatccactt acacgccaca atataaaccc ttggttgctc cagtgattaa
     4141 actcagtttg gtaatcggca gagctcaagt tatgacgggc gacccaatta gggattggag
     4201 atttatccca aaccgcggcg aatcgtggct caccagctac gttataccca catatgtcta
     4261 tcaatcgaaa cccttgcgaa atgaggttat taaattcaga ttgatattga gccgcggtca
     4321 acccatggcg agcgcgccac tcgggaccag cagttttttc ccaaagagat gcataacgat
     4381 ctgtgccaga cacttcaaaa ccgctaacgc gcactgggcg gtatccttga gcagccaggt
     4441 tcgtaaataa ggcttgatga tcccctgagg atagtccgtg cctagcctgc cactcgggac
     4501 ctggcgactg ttcccagata gcactataac gatcgactcc acttactccg taaccactaa
     4561 tatcaatgag tcgatatcct tgagatagta gcgaattgaa ttcaacctga aattgggtgg
     4621 atgtaagtcc atgccgagct tgccatgcat atgccatata acacactcct cataacattg
     4681 tttaaagaaa ataaagtaat agattaagtt gatatgggtt tctgatctga aaataggtta
     4741 gcttaatttc tttattttca atagttatag ttatgcttta ttactccact tcaatcccaa
     4801 aaattcgtga ccaataggaa cttattttgt tgaaatttgt ggttctggcg caaaaactct
     4861 aaagctcttg caagtaatgc cctaaacttg gacttacgga tagtattatt ctaaaacagg
     4921 tgtgtgattg acatgaaaaa gcttgagagg gactttaata aattcaattg tactaaaact
     4981 gcggaggaac attgggcgga acgtgaaaag ttcctcaatg aattgcaatt gatattaaat
     5041 ataatataag attattaatc aaatttatag gagcttttcg tttaatcatt tttcaagccc
     5101 ttgcttaatt gcaagagcat attcctatta gaaatacaat tttagttaca gttaagttga
     5161 tgggcatgtg gcgaatttag aaggccatgg ggtagcacca atcgccatca agctaagaaa
     5221 aataaatacc ccatcgttat taagttaagt aagatcttcc tccctaaaac gataaaaata
     5281 aacttcagta ctataaaaaa caaaaatttc ccgtttagag aatactctaa aatcttagct
     5341 tgatgattat gtgagcgacc ccaattataa gaatctctta atccagttta ataaacaaca
     5401 atttcagttg gtagcaggtt ggctaaagca ggcatttgag atgtattcta cctttacttc
     5461 tttttgaatc atacttctct cctgttttta atacaataag cctgtaaaag tgggttctac
     5521 gtattcttct aggttatcaa aatcaacgat atccaacaca acatcatggg gttttctcag
     5581 gactttgaca ttaccaccga ctacataaat cataattcgt ttttcttcca cctgtttcac
     5641 cctttctaca ccctagtttt acaaaataag aagtcatcaa aacttatatt tattacttcc
     5701 tgttatctcc tctcttgttc tatgcatagt ctatttaaat tttaattggc tactaaaaaa
     5761 gaatttacag gatgaatgaa aaaagccaag tgcattgcac tcggctttat ggtcgattaa
     5821 acaattttac ttcagaagta ttgaactgat gtagataatc tttagtacaa gtatagcctg
     5881 aatcaatgag tgttttcttt tctttttctg taatatcaaa attaattgac gacgtaaaga
     5941 atgtattgat aaatacagta cgagcagcat cttcttttgt tgtttttccg ctcgcttgtc
     6001 ccttaatcat ggtttgagat aaattccata caaaatcaat aagtgaatgg ccaatatgat
     6061 gtttttttgc acttgctata tgtttcaaca tcgcaatctc agcacgagat tctaaacgga
     6121 aacctagtgt ttccttgttg tacacaattt catctgcagg atcaaagaaa gatacattgt
     6181 agttcgtatg taagtttctt aatgcagaat gctgatccgt attacttacc agtttattaa
     6241 cctcgtcgat tgtgtctgtg gtcgtgaagt gttcttcctt acttacaaaa cttttgcgat
     6301 caaataaacg gattgggtag ttatcgagta cacctccgtc cacataataa tcttcctccc
     6361 attccacaga tttgaaaaag aatgggatgg acatcgaaat ccgcacagca acaactaacg
     6421 ggacgtcagg cgtagtctca cgagaaaacg tgcgaagctg atgtgtagaa acgtttgttc
     6481 cttggaaaaa cacgtctttt cctgttttgt catacaactg ctgaaaggtt gtgtccttac
     6541 tccctgtcca tttttcaatt attgcaccta tccactcagt gaaaaaatcc cctttataaa
     6601 tgccaaaacc atcaaaaaac agacgaatat tatcttgaat aaataaaaaa tcatgatctt
     6661 taaatgcttt gaaattcata tctttgtgga tttttacgat atctgcgaat ggaatggaaa
     6721 gacctaaaag taatgcagta attgctcctg cagaagtccc cccaaaccgt tgtatttttg
     6781 gcaaaatctc ttcatcgttt agtgcgcgga tggcaccgat aaaagcgacc ccttttaccc
     6841 cgcccccttc aaatactaaa ttacgaaatg gatatgtcat tatatacctc caatagagaa
     6901 tttttcgatc tgttatagta tcggaagtgg attgaaatat attccaaata atattgaaaa
     6961 agtttcatgc tatgcgatac gtatggcgaa attatcttta ttcaatgaat gaattgttta
     7021 aaaaagaaaa catcggcttg gaatggtaat ttagtaggat aggaaaaagg aagtggggcg
     7081 cttcattagg tgcactgaat aataaccctt tggggaattg tatataacat aacttctttt
     7141 ccgatgcgta ctccaagtaa aatcacgagt atgaaaccaa ggaacaatct aacttttata
     7201 aagccctaat cttattaaca tacaatccag taaaataatt tgtttcagat atgtagaaat
     7261 atggtttaac cagcaatcag tgatcaggtc ttcttaccct aagtaaacct aaatctattt
     7321 gtttccatac gggaagagat aaatagtttt gttcccattc atgcaattaa acggatatgt
     7381 ataattacat atccgtttaa ttgcttttag tgctatattt taatagcgat gaatgctatt
     7441 atttcataag ttgtcaacaa gcgttatgta tgagaaaaaa gaaggggcca aataggtccc
     7501 ttcctttttc tactaattaa ctgaattact ctcaggtaaa aatttaaata gtaatgctca
     7561 tttacaggcg tctaattata cattaaacat gctagctttg cttgatacta gaaaaattaa
     7621 agatggtagc tttaagttac taagaggcaa taattcatca acacgaagat agatttattt
     7681 tggctagttg aactcccccc actttcactt cgtttagaag tgggggattc ctaagtaaag
     7741 agttctatcg aactctaatt gattaggcta accccgcagt ccttgcggtt agaagcctta
     7801 tcgcttcatt ctttagattg atacttgcgt taatatccct atcatggtgt gtacgacaaa
     7861 aaggacagtc ccattcacgt aggtttagat ttttaacgtc tttattttga tatccacaac
     7921 aagaacataa ttggctggaa gcaaatgttt tcgctacgac aatgatttgt ttgccgtacc
     7981 attttgcctt atattccaac atgtttcgaa actgtgacca tgatacctca ctaattgctt
     8041 ttgctaactt atgattcttt aacatattcg atacttgcaa atcctctata ccgataacat
     8101 cgtggttttt gatgatttca gttgaaattt tgtccaagta atcttttcta gcatttgata
     8161 tatattcatg aattctagct acctttactc gttgtttatt ccagcgagaa gatcctttca
     8221 ttcttctaga aagaacacgc tgtgctttcg ccattttctc ttctaatgac cgaaaaaatt
     8281 ccggattttt ataagttgtt ccatctgaca aaatggcgaa attttttagt cctacatcca
     8341 ttccaatgta agaacttgtt ttcggaagtt cttgtacttc tgtttcgact aataatgaca
     8401 caaagtatct accagaagga ttccgtctaa ctgtagcatt tacaatacgc ccctctactt
     8461 cgcgactttt ggcaaatcga acaaggccta gtttcggcaa ctttattttg ttccctacaa
     8521 cagcaatgtt ttcatttgtt tgttttgtgg tataagattg tacattgttc ttcttagatt
     8581 taaagcgcgg tgcgctgttt tgttttttga agaagcgcgt atacgcatcc gcaagattgc
     8641 gaacagacga ctgaatcgca atactatcca cttcttttag ccaaacaagc tctttcttca
     8701 tggcaggtag tttggcagag catgtaccat aggtcaagcc ttttcctgtc tctttgtatg
     8761 cttgatccca taaggatagg aaataattaa atacaaagcg agaacatcca atcgttttgt
     8821 tgattagaat tgcttgtgcc tgatttggat agatacgaaa cttataggct ttattcatca
     8881 ttatttgatt cacctccatt tcggtatatg actattatac accaaacgta cattcgatag
     8941 gtagtcgagt aacactttct gtatgtcgaa caattaagat ggcttcctgc catcccttgt
     9001 tcgaagccaa ttcatctccc acctactcac tgggctgtga cccttcacgc tccttgagga
     9061 aggagtcttc ttggctaaaa tgataaaaaa agacaccttt aaggtgtctt cctcaaaatt
     9121 aataactgca tgttatatca taaactttct caataattat taatttaatc tttactttta
     9181 aatatatcct tttaacatat tttttgagaa acgattcgga tatagagcaa taagcatttc
     9241 atatggactt cctttccaat agcattttaa cggcgtccat aatttttgtt ttattagcca
     9301 tttttgatta aatactttta atagctgttt ttcatccaat ttttctttct cttcaatagt
     9361 ccaccttaat gcctctaatg ctttacgttt agaccaaaac ccaacaggag taactctaaa
     9421 ttcccattct ttaaaacggt ctctatataa atcattaatc attctaaacg gactattccc
     9481 ccaaattatt tgacatgctg atgctaatcc atgggtcttt aaccatttta tattgtatat
     9541 atcaagcaat tgtttagggg ttaatccctc tttttcttca atggtccatt tcaatgcttc
     9601 taatgctttt tctttagtcc agaagttatt aggggtcatg ccaaattccc attctttaaa
     9661 acggcctgga taaagatcat taatcattgc atacgggcta ctattccaat acatagccag
     9721 aggtgcacta agcttatttt ttttcaacca ttttttcccg tataatccta agagcttttc
     9781 ttgtgaagga ccctcttttt cctcaataat ccatttcaaa attgttaatg ctttttcttt
     9841 agtccaaaaa tttaaaggag tcataccaaa ttcccattct ttgaactgat ttggatacaa
     9901 atcattaatt gctttaaaag ggctattttc atacctatgt ttaagtaatc ctcgtagcct
     9961 atactttaca agtaattttg tgttccactt ttttcttata tcttccttat tccattttaa
    10021 aatactgtta agcaaatatg ttaataccct ccgcgccatt ttattatcta tatcttcttt
    10081 ccatgtattt ggaggaaatc taattctttt tccatctaat atttcctgat aaatatcttc
    10141 tatagaaact tcttttttta acaatatata aatcctccta cagtttgtat taatatttat
    10201 acactatcat catcttcaat caatatatct atattaaaaa atataattaa aatacccatt
    10261 aaaatcaaca agaaaattat tttttaaaaa atttctaata attaaccaag taaatattta
    10321 ttttaatttt ctaattatta gtttatacta agttagtatt attatattta tatattagga
    10381 gtgtattcgt atggagaaga ttagtaaaag cgatattatt cgtactgttc cgcaaagcgg
    10441 ttttttcgga caccctaagg ggctgtttac cctattcttt acagagtttt gggagcgatt
    10501 ctcttactat gggatgagag caatattact ttactatatg tattattcaa tctcacaagg
    10561 tggtttaggg atggatcatg cgaccgcagc gtcaattatg gccatctatg gatctctcgt
    10621 ttacatgtct ggcattattg gtggctggat tgcagatcgt ctactaggtt caagtagaac
    10681 aattttttgg ggtggagttt taattatgat aggtcacctg attttagcac taccaggaac
    10741 tataagtact ttattcatat ctatggtgtt tttagtactt ggtacaggtt tattaaaacc
    10801 taatgtatct agcattgttg gtgatttata tagtgaaaca gatactcgac gtgactcagg
    10861 tttcagtatt ttctatatgg gtattaattt aggtgcactt ttatcccctt ttgttatagg
    10921 aacacttggt caaaagtata acttccacct aggttttggt gtcgctgcaa ttggtatgct
    10981 attaggctta attattttcg ttcttacaaa aaagaaaaat cttggtttag caggaacaca
    11041 agtaccaaat cctttaacag acatcgaaag aaaaaaagtt attatacagt ttagtattgg
    11101 tacaatagct atagtactat tttgtatttt aacgatacca acaggtattt taactattaa
    11161 tcgattcact ttattagtaa gtatcttagg tattctcatt ccaactggtt actttattct
    11221 tatgtatcgt agtccgaaaa ctacaaaaat tgaaaagtct cgtttaattg cttacatacc
    11281 attatttatt gctgctacaa tgttttgggc tattcaagaa caaggagcta tcattttagc
    11341 ttcatatgct gatcagcgta ctcaattaaa gttggccggg tttgaacttc aatcttcatg
    11401 gtttcaatca ttaaacccta tttatattgt tttgttagca ccagtatttg cttggttatg
    11461 ggtaaagctt aattcacgtc aaccaagtac ggctaataaa tttgctattg gtttattatt
    11521 tgctggttta tccttcttag ttatgattat ccctgcatat atacatggta cagaatctct
    11581 tgtaagccct atgtggttag cgattagctt cctgttagta gtaatcgggg aactttgttt
    11641 atcgcctgta ggtttatcaa ttacaaccaa attagcacca gctgcgttct ctgctcaaac
    11701 aatgagtctg tggttcttaa ctagtgcagc tgcacaagct ataaatgctc aaatagttcg
    11761 attatataat cctgaaacag aaattattta tttcggtgta attggtggcc tttcaatcat
    11821 tttaagtgta attttatatt ttatggcccc aaagattcaa gttttaatga aaggcataaa
    11881 ataaaaaaaa ctgtgctaaa aacttgttat aatgcaagtt tttatgcaca gtttttttga
    11941 ataaccatta attatttata tattttttgt attctagttc ataatcatat tgattatgta
    12001 aatagcttat ctaataactt tgaacgtcct ctactgtata ttctatcctt ttatttactt
    12061 ttgagagaga aaaaattaac cgagaactca ctataaattt cgcgaaaaag gaattgatga
    12121 gagagtgcca aaatcaccag gaccaccaag tggaggcgga ggtactactt gctttgtgcg
    12181 atgcaattcc aagcagttta gctttaggta acgcgctaaa tacggctgta gaaaactttc
    12241 acctacaatt aaattataat ttgagcccaa ctgacacagt aatacttgtc gttaaaagtg
    12301 ttaatcttca gactttcaca aatcaaggaa cagagctagc aactacaaat aatataacag
    12361 taagctgtga ttttgcattt gctaatttta tagacttgca agtaacttct gaaccaaccc
    12421 tataatacta actaaaaaga ccgtccccca ccctcgtttt acaattaata ctccttagta
    12481 actttttatt aaaagagtat attgcaacaa tttttcattt ggaaattaat ttatcaatga
    12541 ctaggtaaag ataatttctc tataatttct ctccactcaa actcataagc ctctggactt
    12601 cttataacat taattggaat gtttatacca ttcatatttt taatattaaa agtaaatctt
    12661 ataacgttta atggtaacaa tttattatca atacttttca ttatttcttg tatagaaaaa
    12721 tccggaaact caatcgtaat atactctaag tttggagtat attcttcttt tgttatcttt
    12781 ttatatccat acttgctatc atacacatta ataattgaat gtaaaaatga gatagcttct
    12841 tcctttttct tagaattcat atcatataaa taatagactt gattatctat ataaaccctt
    12901 gcaagatact tctgttctaa taaattttct ccagtaaaat aaaaatttgg gttatttttt
    12961 aaatcactta ttaactcctc acatttagga atctgatgaa gccaagaact acatactaca
    13021 atactctcat cgattatttg cgcagtaata ttcatacagc gtaatttatt taatgccaag
    13081 atacgatgtg ttccatctaa aacaagatat ttgtttgatt caattctcat aacaattggt
    13141 aggttactca aggctttttg agcatcaata ttacttttaa ttttaattaa ttgttcctca
    13201 ttatattctt caaaaaaaat taattcttca tatggaataa atttgatttc atagaccatg
    13261 attttagcac ctctcttaat ttcctaaact tcaatgtttg ttatcaacga taaaaatctt
    13321 atttttaggg gatacaatat taaagccaaa cttctaagca actattaatc aaataaagga
    13381 actgtaatta aaaccgttta cagttcctct tgataaaaaa gcttaatata agcttagcac
    13441 ttttcctaat ccctcttgtt tcgccttctc ataaatggca tgagcacatg caatatcttc
    13501 aatagccatt cccataggat ttaatataat aatttcttca tcggtcactc ttcctaattt
    13561 tttaccagtt ataatatcgc caagttctgc atggagcatt tcttttgaga atttcccttc
    13621 tacaacaagc tgattaatta ccttcttttc tctatttgct tgatcccaat catctacaat
    13681 taccttatca gcctttaaaa aagcttcttt ttctatatcc attatagaga tattacttac
    13741 aaaagttcct ttttgtatcc aatctgcttc tatatatgga gtgtctgcta ccgtgcatgt
    13801 tacaagtacc tcagcttgtt ctaccgcatt ttttgctgat tcttttacct gaaaatcgac
    13861 atgactataa cgttctttca tttcttgagc aaaagctttc gcttttacat tatccaaatc
    13921 aaatagatta atttgtctaa tatgattaaa ctgttctagt aaagaattta tgtgcatttt
    13981 agaaattaat ccgcaaccga taattgatac attttgaaat ccttttttcg ccaaatattt
    14041 tgctgcaact acagtaacag ctgctgttct catagaacta ataacactac cttctaaaat
    14101 ggcaattgga aaatttgttt ctggatcatt taaaataatt agtgcactag ctctttccat
    14161 attttttttg gtgggattat caaacttact tccgatccat ttaatccctg aaatagaagg
    14221 ttctccaaga tacgctggca tagcaataat tctatctgcg atatgctctc cttctttatt
    14281 agtctttaaa taaggcttta aaggttgact aaagttcctt tctgaatgta atgttagtgc
    14341 ttgtgtaata gcttgcacat aaatgttggc cttataacca cctaaatcaa taatattctg
    14401 tttactaaga taaattaatt ctggttttgt ttctaataac ataattaaca ctcccttaaa
    14461 ataatatcta ttttttttaa actaaaatgg aatcattaat tgattgtggt aattcaatat
    14521 catgttcaac taaccattca tctgaaaata tagtattata atatcgatca cctctatctg
    14581 gtaatatcgc taatactttt ttagactttg gtagtttatc tattagtctt agagcagcac
    14641 atacaactgc accagaagat ccaccaacta atatcccttc ttttgataca agctctcgtg
    14701 ttataaaaaa ggcatctgca tcattaacgt aacttagatc atcataaaga cttcgattca
    14761 catttccagg gacaatcgca tttcctatcc ccaaaagcct tcttacccca ggctttcctc
    14821 cgaatataac tgatcccaca gcatcgacag ccataacttt tacagatgga tttaattttt
    14881 tcattccttt tgcaacaccc gtaattaatc cagcagtacc tacaggacat acaatccaat
    14941 ctaaagtatc attaaaatca tcataaattt cttgaactaa agtatgcacg taggcctccg
    15001 gattttcagg attgttatat tgattaggcc aataagctcc atcaatagaa ttcgctaaat
    15061 cttgtgctat tacgagtctt gcttttaagt agtttccgtg ttcatctgat acatcaacca
    15121 tatggacttg ggcaccatat gatttaataa gattcagatt cactttactt attttagggt
    15181 caactacaca aatcatttta tatcctttca tctttgctac taacgctaaa ccaattgcaa
    15241 gattcccaga agaggattca acaattgttc ctcctggctt taactgtcca ctattttcag
    15301 catattcaat tactcgcttt gcaactctat ctttcgaact tcctccagga ttaaatcctt
    15361 ctaattttac atatagttga gcactctcag gtgggaccag cttattaatt tttacaatag
    15421 gtgtatctcc tattaatgaa agaacgtcat catgaatcat gtttttgccc cttttcacat
    15481 taattagctc atttaatttt tataaaaaac caataccctc attccaatct tttttaactt
    15541 aaacttcaca taataagacg ggatcagtat acaacctaat tgattttccc cataattcca
    15601 acagttcatt ccaatcctcc tctacaaaat cttctcggat tagaaaactt aacggaatgt
    15661 ttaaattcaa aatacgtcca caatttaatt taaatttagt tacacctgca ggtagtagta
    15721 aattcgactc tactaattgt ttgatttgat acaactctag caaagtatag ctaaacacta
    15781 tgccgtctga aggaattaat tcgtcatttg ttattctagt aaacggatat ttatttgtat
    15841 aagcattaac aatttctttc caaattctaa tttttatttc catatctttt tccatttctg
    15901 gattaattgc cagatcatac tttattccat taacaaacac tgttgcaatt aaattttcac
    15961 tttcaatatt ttcacttaaa aaaatattag aattactttt taatacctct aatactccct
    16021 cactatctga tatatgatgt gaccatgctc caattgataa ataatctgct ttaacgacct
    16081 gaacaaccat ccttttacaa tttaattgct tcaaagataa cgtcctatgt gctccatcaa
    16141 ggattagata tttatcttca gctaattgta aagcgattgg gggattcttt aaaacctgtt
    16201 catttttaat cttatgactt aactttttta acctttcatg ttcatattct tcatgtaaat
    16261 atatttgctc cgttggaata acttttaagt catttagcat accatttcct cctggaaact
    16321 attatcatca tttaagataa agttttatat aaatctatta attctttaag aagaatttct
    16381 ttaatactaa atgtatattt tttatttttt gaacatttta aagcaactga gacaatataa
    16441 tcaggatcta ataaaaagga attatataaa aaatcacctc ctttaataat cccgctattt
    16501 tctggtacag aaatagatac aggatgtgtg taaaaccctg ttcttctaca tctaaaacat
    16561 tcaaacacta attaaaatct gatattttta tattttcagg tattttcaaa tataaaacgg
    16621 atactctttc ttgcattatt cttcctcaca ataatatttc atttatcatc catattttta
    16681 aataaattca tttatttgtt ttctaataat tccttagaaa ttaaatatag caactctttt
    16741 tcaacttctt ttaaatagaa atgatttcct ggtaacatct gaactttaaa tgctttacta
    16801 gtgtgtttct cccactgttt tagatgctct aaacgaactc tactatcctc tgttccacca
    16861 tacgccacaa taggacaatt taatttattt ctttctttat atacatacgt ttcacacact
    16921 tcaaagtccg cacgtatagt aggtataatt aattccatta actcttgatt acttaatatt
    16981 tcttcagatg ttcctcccat ttcttttaat ttctcaataa aaataggtac agataaatca
    17041 tgcatattat ttgttattgg cgatagatgc ggtgccctaa aactagaaat aattaacttt
    17101 tctggaaatg tcgaaaaatt ttcttttaga taccatgaca tttcataact taataatgcc
    17161 cccatactat gtccaaaaaa tacataaggc ttatctaata aaggaagaat ctctactgct
    17221 aattcacata taatagattg gatattattg aatggtttct ccataattct attttctcta
    17281 cctggtaatt gaacagcaat tacctcaacc tgttccggta atttagttgc ccatttttta
    17341 aaattactgg cacttcctcc tgcataatga aaacaaaata atctcatact agttggaaaa
    17401 gaatttacct tattatattt tttgaaatat agcgatggtg aataaatatt attcatatca
    17461 aacacacctt taaaaattaa tttaaattaa aactagaatt gtataatttt ttacttatct
    17521 cttctaagaa tatatcaata tgatatgcaa tatcttcagc atgcgcagac tgcatcatac
    17581 tataatgagt tgcgtttagt tctataactt ttaattcttt ttgcacatat ttcccccaaa
    17641 ataaggtatc atcttctaca tgattatttt ctttatgtgg atgatttgca gtaaaaagta
    17701 atatatcttg atccaatttt tccggttgat aactccgata agcattaaga tttgtttgaa
    17761 atacctgaat catcttttct agatcttcaa cttgtatatc ttgcttaaac tttgaatgat
    17821 ttttcaaata acttattaca tagcgaatac gttcatcttg catacttaaa tttttgtatc
    17881 ctaatattaa ttcttcggat acttctaggt tatattgctt cattaaatca ttcataaata
    17941 cggctaagtc atcccattct gataaatcat tatatatatc tggtgcccat gtatctagca
    18001 atataatggc ttcaaccttt attcctttct caactaattg tctcgccatt tcaaaagcta
    18061 caaccccacc aaaagaccat ccaccaataa tataaggacc ctcttcctga acattctgca
    18121 tttccttaat ataaaaatta gccatttctt ttatatttgt taatattgtt tcttcactct
    18181 ctaatcctag cgactgaaaa gcgtaaaaag gacgcttatc ccctaaatat ttagctaatt
    18241 caaaataact tataatatgt ccaccaatag gatgaacaaa gaaaataggt ctcttattcc
    18301 cctttggttg aagaggtact attgaattat taactttata aattttattt ctatctattt
    18361 gtttcgctaa tgcagcaatt gttccacttt gaaaaataat agttaacgga attttcacat
    18421 taaacgtctt tttaattttt gacattaatt gaacagccat tagagagtgc ccaccaattc
    18481 cgaaaaaatt gtcatataca gaaatcccag aataacctag tagctcttcc cagatttcaa
    18541 ctaatttttt ttcaatatct gttttgggtt cgatatattc tgttcgttta attaaggaca
    18601 tatctggagc aggcaatgcc tccttgtcta cttttccatt aattgttaaa ggtatttttt
    18661 ctaacaacac aaaagcactc ggtatcatgt aatccggcaa ctgctctgcc atatagttgc
    18721 taagtttctc aacctcatca gctgtaaggt catctccaat aatgtaccca actagtcttt
    18781 tgtcacctgg aatgtcttct cgaactaaaa caataccctc acgtatttta gaatattgat
    18841 taagtatagt ttcaatctct cctaattcaa ttctaaatcc tctcaattta acttgttgat
    18901 ctatgcgttc aatatactca agatttccat ttggtaaata acgtactata tctccagttt
    18961 tatacaattt ccgttcctca ttactattga atggagaagt aataaatctt tccgcagtta
    19021 attctggtcg tttataatac ccttttgcta atcccactcc tccaatatat agttccccat
    19081 gaacaccaat aggtacaggt tgtaaagagg tatctaatac atacatttca gtattaaaaa
    19141 taggctttcc tataggtatg gatttactat aattctctcc tggttttact atataattag
    19201 tacatccaac tactgcttca gtcggtccat attcatttat tatgactgta tttggagcat
    19261 gctttaccca aaaatcaaca tgttttgcga gtagaggctc tcctcctact acaaaagtat
    19321 ctgtacaaaa agcagcatct tcttcagaaa cttgatgaga taataactct aaatgggctg
    19381 gtgttagctt tataacacta tattttcttt tccgaaccca tcttcctaat tcttctaaat
    19441 ctaatccctc aggaaataaa actactgttt ctcctataca taatgg
//
