LOCUS       NZ_MOOL01000050         9253 bp    DNA     linear   CON 03-APR-2024
DEFINITION  Bacillus thuringiensis serovar xiaguangiensis strain BGSC 4BN1
            contig000050, whole genome shotgun sequence.
ACCESSION   NZ_MOOL01000050
VERSION     NZ_MOOL01000050.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis serovar xiaguangiensis
  ORGANISM  Bacillus thuringiensis serovar xiaguangiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Comparative genomics of Bacillus thuringiensis reveals a path to
            pathogens against multiple invertebrate hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, 1st Shizishan,
            Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.5.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002147905.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 02:08:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,596
            CDSs (total)                      :: 6,434
            Genes (coding)                    :: 6,198
            CDSs (with protein)               :: 6,198
            Genes (RNA)                       :: 162
            rRNAs                             :: 15, 15, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 1 (5S, 23S)
            partial rRNAs                     :: 15, 12 (16S, 23S)
            tRNAs                             :: 114
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 95 of 236
            Pseudo Genes (incomplete)         :: 153 of 236
            Pseudo Genes (internal stop)      :: 70 of 236
            Pseudo Genes (multiple problems)  :: 63 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:39:27
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9253
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOOL01000050.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..5537)
                     /locus_tag="BK733_RS22530"
                     /old_locus_tag="BK733_21480"
     CDS             complement(<1..5537)
                     /NRPS_PKS="Domain: AMP-binding (268-662). E-value:
                     1.2e-107. Score: 351.9. Matches aSDomain:
                     nrpspksdomains_BK733_RS22530_AMP-binding.1"
                     /NRPS_PKS="Domain: PP-binding (770-837). E-value: 3.6e-18.
                     Score: 57.6. Matches aSDomain:
                     nrpspksdomains_BK733_RS22530_PP-binding.1"
                     /NRPS_PKS="Domain: Condensation_LCL (854-1141). E-value:
                     2.1e-103. Score: 337.4. Matches aSDomain:
                     nrpspksdomains_BK733_RS22530_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (1306-1708). E-value:
                     1.4e-111. Score: 364.9. Matches aSDomain:
                     nrpspksdomains_BK733_RS22530_AMP-binding.2"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="BK733_RS22530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_21480"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_140157172.1"
                     /sec_met_domain="PP-binding (E-value: 2e-18, bitscore:
                     53.9, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2.2e-87, bitscore:
                     280.0, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 6.6e-122, bitscore:
                     394.0, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPNTLTPELYINEDLEKEKHYWVEKLSGDIEFSSFLPDLETVKS
                     EGTVKFSIPIPIEVSKKINKISNYSKWGAFLILITSINVILQKYTRNNDILIGTTGLKN
                     GMDDLTLFRNTVSPDMTWKELLLNVKNTVNDAVDHQKLNISSLLSSMGLLHSETEDIDF
                     PVLINFENLREKNCTSDTRAKIAFHFSQNDDNQITGEIIYQKVYSSDFIRNLATQFVMT
                     IEQFIDNPEYKVGDLDLVTEREKEKLLKEFNQTTVNFGNKKLLHELVEEQVKKSADRVA
                     IRFEESEITYSELNKRANQLAALLRQKGVSSNTFVGLMISPGIEMAIGLLGILKAGGAY
                     VPIDPKYPLERQNAIIQDSGLGLLLKETEDRTKLDFNGEIIDLEVLQKNTKNQGNLPSI
                     NKGEDLAYSIYTSGTTGKPKGVMIQHKSITNNILWRKNEYKLTEKDRVLQLFSFSFDGF
                     VTSFFTPLVSGAQIILVGNDDVKNPPYLYNIVQRHNITHFISTPSLYSSLLAEIQYEDQ
                     LCLRIITLAGEEVTANLLRESSEKLKDVEIANEYGPTENSVVTTVYRNLKVDSKISIGK
                     PIANNYVYILDNEQKLQPIGMPGELCISGESLAKGYVNNQPLTNEKIVPNPFFSGSLMY
                     RTGDLARWLPDGNLEYLGRLDEQVKIRGFRIELKDIEYQLKEYQSIDDVLVIALKDSEE
                     SNFLCAYYMSSEEIPVSTFRNFLEKRLPGFMIPDYFVKIDQFPLTINGKIDKKSLPEPK
                     QAISGESTYHAPWNELEEKLITIWSQILGIEKEKIGISDNLFNLGMHSLKIASFVSRIY
                     REFEISIPIHIIFQLSTVKEQSAYISQIKSEKIYAIEKVEEREDYLLSSAQKRIYMLQA
                     LNKFQTSYNMPKAMIIEGTLDIKRVESTFQMLLKRHESLRTFFKVKDGNPVQQIHDYNP
                     IEIELLHLRDKKISELIANFVEPFDLYNNSLIRLKLVELKENKHLLLIDMHHIIADGQS
                     IQLLVNEFVQLYEGKELPELHIQYKDYAAWQNNFLKSEELQSQEHYWLSQFSEEYPVLQ
                     LPTDYPRPSVKGSKGDSISVYIGKTLTRHMHQIAARKDTTLFMLLLAAYNVLLSKYGDQ
                     EDIIVGTPISGRNHLDVENVIGMFVNTLALRNRPKKDKTFLEFLDEVKKSTLNAFENQD
                     YPFELLVEKLKVQRDTSRNPIFDTMFTFQDSSYSKISIDGLQFSTVDIELNSSKFDLSL
                     YITEVEEGLHAQFEYSTELFKKDSIERLSEHFLAILEKISESSNIKLAEIEMINREEEK
                     LLLHDFNQTQYDNHNNTLLHELFEKQVERIPDKVALQFAGRTMTYKELNEQANQLARKI
                     RKLKSQGEEEFLVSVAMERSFEMVKTILAILKAGGAYVPIDPHHPSKRIETIIKDSQTC
                     VLLTQDKFKEKLSFYHEKVIVVDDQKDHKEEVSNLSQVSNLENLAYVIYTSGSTGKPKG
                     VMIEHRSVLNTLFGLQRKFPITEEDAYLLKTPFIFDVSVAELFGWMLGGGRLVILEPEM
                     EKDPVELYKVIQKERVTHVNFVPSMLQAFVDMVPDKQLQELTDLKYIFVAGEALSPKLA
                     KSARMLLSHTDLYNLYGPTEASIYTTGYALDNNLDMSILPIGKPLDNTGVYILSNEGKL
                     QPIGIPGELCIAGEGVARGYLNDEKRTKEKFISHPYKKDTVLYKTGDLVRWLPDGNIEY
                     LGRKDFQIKVRGYRIEIGEIEYCIMRREGVREVVVIDREDPHGRKYLCAYIVSDVEWSN
                     KEWHQYMREKLPEYMIPTNFVHLENMPLSLNGKLDRKALPKEEVILNQEEYIAPRNEME
                     AKLVQIWSEVLGIEDNRIGVTNDFFELGGHS"
     source          1..9253
                     /country="China"
                     /db_xref="taxon:180870"
                     /isolation_source="bean"
                     /mol_type="genomic DNA"
                     /organism="Bacillus thuringiensis serovar xiaguangiensis"
                     /serotype="H51"
                     /serovar="xiaguangiensis"
                     /strain="BGSC 4BN1"
                     /submitter_seqid="contig000050"
     region          1..9253
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9253
                     /SMILES="NC(C)C(=O)NC(Cc1ccc(O)cc1)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..9253
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:9247](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..9247)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(384..449)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22530_0015"
                     /evalue="4.50E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="BK733_RS22530"
                     /protein_end="1718"
                     /protein_start="1696"
                     /score="34.7"
                     /tool="antismash"
                     /translation="YLGRKDFQIKVRGYRIEIGEIE"
     aSDomain        complement(414..1619)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS22530_AMP-binding.2"
                     /evalue="1.40E-111"
                     /label="BK733_RS22530_AMP-binding.2"
                     /locus_tag="BK733_RS22530"
                     /protein_end="1708"
                     /protein_start="1306"
                     /score="364.9"
                     /specificity="substrate consensus: Ala"
                     /tool="antismash"
                     /translation="FEKQVERIPDKVALQFAGRTMTYKELNEQANQLARKIRKLKSQGE
                     EEFLVSVAMERSFEMVKTILAILKAGGAYVPIDPHHPSKRIETIIKDSQTCVLLTQDKF
                     KEKLSFYHEKVIVVDDQKDHKEEVSNLSQVSNLENLAYVIYTSGSTGKPKGVMIEHRSV
                     LNTLFGLQRKFPITEEDAYLLKTPFIFDVSVAELFGWMLGGGRLVILEPEMEKDPVELY
                     KVIQKERVTHVNFVPSMLQAFVDMVPDKQLQELTDLKYIFVAGEALSPKLAKSARMLLS
                     HTDLYNLYGPTEASIYTTGYALDNNLDMSILPIGKPLDNTGVYILSNEGKLQPIGIPGE
                     LCIAGEGVARGYLNDEKRTKEKFISHPYKKDTVLYKTGDLVRWLPDGNIEYLGRKDFQI
                     KVR"
     aSModule        complement(414..2975)
                     /domains="nrpspksdomains_BK733_RS22530_Condensation_LCL.1"
                     /domains="nrpspksdomains_BK733_RS22530_AMP-binding.2"
                     /incomplete
                     /locus_tags="BK733_RS22530"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(531..620)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22530_0014"
                     /evalue="5.30E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="BK733_RS22530"
                     /protein_end="1669"
                     /protein_start="1639"
                     /score="47.5"
                     /tool="antismash"
                     /translation="PIGIPGELCIAGEGVARGYLNDEKRTKEKF"
     CDS_motif       complement(729..755)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22530_0013"
                     /evalue="2.00E-01"
                     /label="NRPS-A_a5"
                     /locus_tag="BK733_RS22530"
                     /protein_end="1603"
                     /protein_start="1594"
                     /score="5.1"
                     /tool="antismash"
                     /translation="YGPTEASIY"
     CDS_motif       complement(1140..1199)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22530_0012"
                     /evalue="1.30E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="BK733_RS22530"
                     /protein_end="1466"
                     /protein_start="1446"
                     /score="36.1"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGKPKGVMIEH"
     CDS_motif       complement(1386..1427)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22530_0011"
                     /evalue="4.50E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="BK733_RS22530"
                     /protein_end="1384"
                     /protein_start="1370"
                     /score="19.7"
                     /tool="antismash"
                     /translation="LAILKAGGAYVPID"
     CDS_motif       complement(1956..2072)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22530_0010"
                     /evalue="2.60E-20"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="BK733_RS22530"
                     /protein_end="1194"
                     /protein_start="1155"
                     /score="64.8"
                     /tool="antismash"
                     /translation="KSTLNAFENQDYPFELLVEKLKVQRDTSRNPIFDTMFTF"
     aSDomain        complement(2115..2975)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS22530_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.10E-103"
                     /label="BK733_RS22530_Condensation_LCL.1"
                     /locus_tag="BK733_RS22530"
                     /protein_end="1141"
                     /protein_start="854"
                     /score="337.4"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="EDYLLSSAQKRIYMLQALNKFQTSYNMPKAMIIEGTLDIKRVEST
                     FQMLLKRHESLRTFFKVKDGNPVQQIHDYNPIEIELLHLRDKKISELIANFVEPFDLYN
                     NSLIRLKLVELKENKHLLLIDMHHIIADGQSIQLLVNEFVQLYEGKELPELHIQYKDYA
                     AWQNNFLKSEELQSQEHYWLSQFSEEYPVLQLPTDYPRPSVKGSKGDSISVYIGKTLTR
                     HMHQIAARKDTTLFMLLLAAYNVLLSKYGDQEDIIVGTPISGRNHLDVENVIGMFVNTL
                     ALRNRP"
     CDS_motif       complement(2124..2213)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22530_0009"
                     /evalue="1.40E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="BK733_RS22530"
                     /protein_end="1138"
                     /protein_start="1108"
                     /score="49.6"
                     /tool="antismash"
                     /translation="DIIVGTPISGRNHLDVENVIGMFVNTLALR"
     CDS_motif       complement(2469..2504)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22530_0008"
                     /evalue="4.90E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="BK733_RS22530"
                     /protein_end="1023"
                     /protein_start="1011"
                     /score="15.9"
                     /tool="antismash"
                     /translation="QYKDYAAWQNNF"
     CDS_motif       complement(2535..2600)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22530_0007"
                     /evalue="1.10E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="BK733_RS22530"
                     /protein_end="1001"
                     /protein_start="979"
                     /score="30.7"
                     /tool="antismash"
                     /translation="MHHIIADGQSIQLLVNEFVQLY"
     CDS_motif       complement(2796..2909)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22530_0006"
                     /evalue="2.40E-11"
                     /label="C2_LCL_024-062"
                     /locus_tag="BK733_RS22530"
                     /protein_end="914"
                     /protein_start="876"
                     /score="36.0"
                     /tool="antismash"
                     /translation="TSYNMPKAMIIEGTLDIKRVESTFQMLLKRHESLRTFF"
     aSDomain        complement(3027..3227)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS22530_PP-binding.1"
                     /evalue="3.60E-18"
                     /label="BK733_RS22530_PP-binding.1"
                     /locus_tag="BK733_RS22530"
                     /protein_end="837"
                     /protein_start="770"
                     /score="57.6"
                     /tool="antismash"
                     /translation="EKLITIWSQILGIEKEKIGISDNLFNLGMHSLKIASFVSRIYREF
                     EISIPIHIIFQLSTVKEQSAYI"
     aSModule        complement(3027..6937)
                     /complete
                     /domains="nrpspksdomains_BK733_RS22535_Condensation_LCL.1"
                     /domains="nrpspksdomains_BK733_RS22530_AMP-binding.1"
                     /domains="nrpspksdomains_BK733_RS22530_PP-binding.1"
                     /locus_tags="BK733_RS22530"
                     /locus_tags="BK733_RS22535"
                     /monomer_pairings="Tyr -> Tyr"
                     /monomer_pairings="Tyr -> Tyr"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(3522..3587)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22530_0005"
                     /evalue="1.10E-10"
                     /label="NRPS-A_a8"
                     /locus_tag="BK733_RS22530"
                     /protein_end="672"
                     /protein_start="650"
                     /score="33.6"
                     /tool="antismash"
                     /translation="YLGRLDEQVKIRGFRIELKDIE"
     aSDomain        complement(3552..4733)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS22530_AMP-binding.1"
                     /evalue="1.20E-107"
                     /label="BK733_RS22530_AMP-binding.1"
                     /locus_tag="BK733_RS22530"
                     /protein_end="662"
                     /protein_start="268"
                     /score="351.9"
                     /specificity="substrate consensus: Tyr"
                     /tool="antismash"
                     /translation="VEEQVKKSADRVAIRFEESEITYSELNKRANQLAALLRQKGVSSN
                     TFVGLMISPGIEMAIGLLGILKAGGAYVPIDPKYPLERQNAIIQDSGLGLLLKETEDRT
                     KLDFNGEIIDLEVLQKNTKNQGNLPSINKGEDLAYSIYTSGTTGKPKGVMIQHKSITNN
                     ILWRKNEYKLTEKDRVLQLFSFSFDGFVTSFFTPLVSGAQIILVGNDDVKNPPYLYNIV
                     QRHNITHFISTPSLYSSLLAEIQYEDQLCLRIITLAGEEVTANLLRESSEKLKDVEIAN
                     EYGPTENSVVTTVYRNLKVDSKISIGKPIANNYVYILDNEQKLQPIGMPGELCISGESL
                     AKGYVNNQPLTNEKIVPNPFFSGSLMYRTGDLARWLPDGNLEYLGRLDEQVKIR"
     CDS_motif       complement(3672..3758)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22530_0004"
                     /evalue="3.80E-11"
                     /label="NRPS-A_a6"
                     /locus_tag="BK733_RS22530"
                     /protein_end="622"
                     /protein_start="593"
                     /score="35.2"
                     /tool="antismash"
                     /translation="PIGMPGELCISGESLAKGYVNNQPLTNEK"
     CDS_motif       complement(3858..3890)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22530_0003"
                     /evalue="1.10E-01"
                     /label="NRPS-A_a5"
                     /locus_tag="BK733_RS22530"
                     /protein_end="560"
                     /protein_start="549"
                     /score="5.8"
                     /tool="antismash"
                     /translation="EYGPTENSVVT"
     CDS_motif       complement(4263..4322)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22530_0002"
                     /evalue="8.60E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="BK733_RS22530"
                     /protein_end="425"
                     /protein_start="405"
                     /score="30.6"
                     /tool="antismash"
                     /translation="AYSIYTSGTTGKPKGVMIQH"
     CDS_motif       complement(4506..4547)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22530_0001"
                     /evalue="7.60E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="BK733_RS22530"
                     /protein_end="344"
                     /protein_start="330"
                     /score="19.0"
                     /tool="antismash"
                     /translation="LGILKAGGAYVPID"
     gene            complement(5603..9247)
                     /locus_tag="BK733_RS22535"
                     /old_locus_tag="BK733_21485"
     CDS             complement(5603..9247)
                     /NRPS_PKS="Domain: AMP-binding (177-579). E-value:
                     6.7e-112. Score: 366.0. Matches aSDomain:
                     nrpspksdomains_BK733_RS22535_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (685-753). E-value: 3.6e-22. Score:
                     70.3. Matches aSDomain: nrpspksdomains_BK733_RS22535_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (770-1057). E-value:
                     1.1e-90. Score: 295.7. Matches aSDomain:
                     nrpspksdomains_BK733_RS22535_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="BK733_RS22535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_21485"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_088021042.1"
                     /sec_met_domain="AMP-binding (E-value: 3.1e-122, bitscore:
                     395.1, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.5e-20, bitscore:
                     60.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.8e-75, bitscore:
                     240.8, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFVNTLALRNRPKKDKTFLEFLDEVKKSTLNAFENQDYPFELLVE
                     KLKVQRDTSRNPIFDTMFTFQDSSYSKISIDGLQFSTVDIELNSSKFDLSLYITEVEEG
                     LHAQFEYSTELFKKDSIERLSEHFLAILEKISESSNIKLAEIEMINREEEKLLLHDFNQ
                     TQYDNHNNTLLHELFEKQVERIPDKVALQFAGRTMTYKELNEQANQLARKIRKLKSQGE
                     EEFLVSVAMERSFEMVKTILAILKAGGAYVPIDPHHPSKRIETIIKDSQTCVLLTQDKF
                     KEKLSFYHEKVIVVDDQKDHKEEVSNLSQVSNLENLAYVIYTSGSTGKPKGVMIEHRSV
                     LNTLFGLQRKFPITEEDAYLLKTPFIFDVSVAELFGWMLGGGRLVILEPEMEKDPVELY
                     KVIQKERVTHVNFVPSMLQAFVDMVPDKQLQELTDLKYIFVAGEALSPKLAKSARMLLS
                     HTDLYNLYGPTEASIYTTGYALDNNLDMSILPIGKPLDNTGVYILSNEGKLQPIGIPGE
                     LCIAGEGVARGYLNDEKRTKEKFISHPYKKDTVLYKTGDLVRWLPDGNIEYLGRKDFQI
                     KVRGYRIEIGEIEYCIMRREGVREVVVIDREDPHGRKYLCAYIVSDVEWSNKEWHQYMR
                     EKLPEYMIPTNFVHLENMPLSLNGKLDRKALPKEEVILNQEEYIAPRNEMEAKLVQIWS
                     EVLGIEDNRIGVTNDFFELGGHSLNAVTLLTKINKQFNKEIGLRDIFKMSTIKSLAVHL
                     INSKEFMHKPIPKAQKKEYYSLSYVQQRLFILEQTKQMGTSYNTPVLLEIEGELDRHRL
                     EGTLNELIKRHEAFRTSFGFVDGEFVQKIHPNIPFLLEYKVINEADIRLNVEKFITPFD
                     LTKAPLIRAKLFEIDKSKFVLCIDVHHIIADGFSLQLLLNEIIRLYKGDILSEPSLQYK
                     DYAVWQQSSEFQEQLKKQETYWHNLYKGDVLLKKLPVDYPSNNPHSNQRRVLNYKLNQE
                     VCQNLVNLSKANKITLFQMFFALYTILVSKYTNSDDVIVGTPTAGRTHTDVENIVGMFV
                     NTLAIRNNPKADLTFINFLQQVKNNTMLAFENQDYPYEMLLDYLQFSNMNSSQGIINTM
                     FIYKKDKINNHFYNVAGLKLKEYPYESGKAKFDLTVNVVESGDYFNLNIVYKDNIYKQE
                     TIKKIAEDIDLIAKQVTVDTMIKIADIKVEGSQASEEISFPEDLTFSF"
     CDS_motif       complement(5660..5695)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22535_0010"
                     /evalue="4.80E+01"
                     /label="NRPS-A_a8"
                     /locus_tag="BK733_RS22535"
                     /protein_end="1196"
                     /protein_start="1184"
                     /score="-2.6"
                     /tool="antismash"
                     /translation="DTMIKIADIKVE"
     aSDomain        complement(6077..6937)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS22535_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.10E-90"
                     /label="BK733_RS22535_Condensation_LCL.1"
                     /locus_tag="BK733_RS22535"
                     /protein_end="1057"
                     /protein_start="770"
                     /score="295.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="EYYSLSYVQQRLFILEQTKQMGTSYNTPVLLEIEGELDRHRLEGT
                     LNELIKRHEAFRTSFGFVDGEFVQKIHPNIPFLLEYKVINEADIRLNVEKFITPFDLTK
                     APLIRAKLFEIDKSKFVLCIDVHHIIADGFSLQLLLNEIIRLYKGDILSEPSLQYKDYA
                     VWQQSSEFQEQLKKQETYWHNLYKGDVLLKKLPVDYPSNNPHSNQRRVLNYKLNQEVCQ
                     NLVNLSKANKITLFQMFFALYTILVSKYTNSDDVIVGTPTAGRTHTDVENIVGMFVNTL
                     AIRNNP"
     CDS_motif       complement(6086..6175)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22535_0009"
                     /evalue="2.10E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="BK733_RS22535"
                     /protein_end="1054"
                     /protein_start="1024"
                     /score="49.0"
                     /tool="antismash"
                     /translation="DVIVGTPTAGRTHTDVENIVGMFVNTLAIR"
     CDS_motif       complement(6434..6466)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22535_0008"
                     /evalue="6.90E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="BK733_RS22535"
                     /protein_end="938"
                     /protein_start="927"
                     /score="15.4"
                     /tool="antismash"
                     /translation="QYKDYAVWQQS"
     CDS_motif       complement(6497..6562)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22535_0007"
                     /evalue="6.50E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="BK733_RS22535"
                     /protein_end="917"
                     /protein_start="895"
                     /score="28.3"
                     /tool="antismash"
                     /translation="VHHIIADGFSLQLLLNEIIRLY"
     CDS_motif       complement(6758..6871)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22535_0006"
                     /evalue="1.20E-15"
                     /label="C2_LCL_024-062"
                     /locus_tag="BK733_RS22535"
                     /protein_end="830"
                     /protein_start="792"
                     /score="49.8"
                     /tool="antismash"
                     /translation="TSYNTPVLLEIEGELDRHRLEGTLNELIKRHEAFRTSF"
     aSDomain        complement(6989..7192)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS22535_PCP.1"
                     /evalue="3.60E-22"
                     /label="BK733_RS22535_PCP.1"
                     /locus_tag="BK733_RS22535"
                     /protein_end="753"
                     /protein_start="685"
                     /score="70.3"
                     /tool="antismash"
                     /translation="EAKLVQIWSEVLGIEDNRIGVTNDFFELGGHSLNAVTLLTKINKQ
                     FNKEIGLRDIFKMSTIKSLAVHL"
     aSModule        complement(6989..8716)
                     /complete
                     /domains="nrpspksdomains_BK733_RS22535_AMP-binding.1"
                     /domains="nrpspksdomains_BK733_RS22535_PCP.1"
                     /locus_tags="BK733_RS22535"
                     /monomer_pairings="Ala -> Ala"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(7481..7546)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22535_0005"
                     /evalue="2.80E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="BK733_RS22535"
                     /protein_end="589"
                     /protein_start="567"
                     /score="35.4"
                     /tool="antismash"
                     /translation="YLGRKDFQIKVRGYRIEIGEIE"
     aSDomain        complement(7511..8716)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS22535_AMP-binding.1"
                     /evalue="6.70E-112"
                     /label="BK733_RS22535_AMP-binding.1"
                     /locus_tag="BK733_RS22535"
                     /protein_end="579"
                     /protein_start="177"
                     /score="366.0"
                     /specificity="substrate consensus: Ala"
                     /tool="antismash"
                     /translation="FEKQVERIPDKVALQFAGRTMTYKELNEQANQLARKIRKLKSQGE
                     EEFLVSVAMERSFEMVKTILAILKAGGAYVPIDPHHPSKRIETIIKDSQTCVLLTQDKF
                     KEKLSFYHEKVIVVDDQKDHKEEVSNLSQVSNLENLAYVIYTSGSTGKPKGVMIEHRSV
                     LNTLFGLQRKFPITEEDAYLLKTPFIFDVSVAELFGWMLGGGRLVILEPEMEKDPVELY
                     KVIQKERVTHVNFVPSMLQAFVDMVPDKQLQELTDLKYIFVAGEALSPKLAKSARMLLS
                     HTDLYNLYGPTEASIYTTGYALDNNLDMSILPIGKPLDNTGVYILSNEGKLQPIGIPGE
                     LCIAGEGVARGYLNDEKRTKEKFISHPYKKDTVLYKTGDLVRWLPDGNIEYLGRKDFQI
                     KVR"
     CDS_motif       complement(7628..7717)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22535_0004"
                     /evalue="3.30E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="BK733_RS22535"
                     /protein_end="540"
                     /protein_start="510"
                     /score="48.1"
                     /tool="antismash"
                     /translation="PIGIPGELCIAGEGVARGYLNDEKRTKEKF"
     CDS_motif       complement(8237..8296)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22535_0003"
                     /evalue="7.90E-12"
                     /label="NRPS-A_a3"
                     /locus_tag="BK733_RS22535"
                     /protein_end="337"
                     /protein_start="317"
                     /score="36.7"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGKPKGVMIEH"
     CDS_motif       complement(8483..8524)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22535_0002"
                     /evalue="2.80E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="BK733_RS22535"
                     /protein_end="255"
                     /protein_start="241"
                     /score="20.3"
                     /tool="antismash"
                     /translation="LAILKAGGAYVPID"
     CDS_motif       complement(9053..9169)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22535_0001"
                     /evalue="1.60E-20"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="BK733_RS22535"
                     /protein_end="65"
                     /protein_start="26"
                     /score="65.5"
                     /tool="antismash"
                     /translation="KSTLNAFENQDYPFELLVEKLKVQRDTSRNPIFDTMFTF"
ORIGIN
        1 gaatgaccac ccagctcgaa aaagtcattg gtaactccaa tacgattatc ttcaattccc
       61 aatacttctg accatatttg caccaattta gcttccattt catttcgtgg tgctatgtat
      121 tcctcttgat ttaagataac ttcttcttta ggtaatgcct ttcggtctaa cttaccattt
      181 aatgataatg gcatgttttc caaatgcaca aaattagttg gaatcatata ttctggaagc
      241 ttctctctca tgtattgatg ccattcttta ttactccatt caacatccga cacgatatat
      301 gcacataaat attttcttcc atgaggatct tccctatcaa taacgactac ctcacgaacg
      361 ccttcacgtc gcatgatgca atactctatc tccccaattt caatacgata tcctcgtact
      421 ttaatttgga aatcttttct acctaaatac tcaatattcc catctggtaa ccatctgacc
      481 aaatcacctg ttttatataa gacggtatct tttttgtaag gatgggagat aaatttctcc
      541 ttcgttcgtt tctcatcatt gagatatcct cttgctaccc cttctccggc tatacacaat
      601 tcaccaggaa tgcctatcgg ttgtaatttc ccttcattac ttaatatata cacacctgta
      661 ttatcaagtg gttttccaat cggtaaaatt gacatgtcaa gattattgtc tagcgcatac
      721 cctgttgtat aaatactcgc ttccgttggg ccatagagat tatataaatc agtatgcgat
      781 aataacattc ttgccgattt agccaatttg ggcgataatg cctcaccagc aacaaatata
      841 tattttaaat ctgttaattc ctgtaactgt ttatcaggga ccatgtcgac aaatgcttga
      901 agcatagaag gtacaaaatt gacatgtgtc acccgctctt tttgaatcac cttatatagt
      961 tccactggat ccttttccat ttcaggctct aaaataacca gccttcctcc tccaagcatc
     1021 cagccaaata attctgctac cgagacgtca aatataaaag gtgtttttaa taaatatgca
     1081 tcctcttctg taataggaaa ttttctctgt aatccgaata atgtatttaa aacacttcga
     1141 tgttcaatca taacaccctt cggcttacct gtagaacctg aggtatagat gacatatgct
     1201 aaattttcaa gattactaac ttgacttaaa ttagatactt cttctttgtg atctttttgg
     1261 tcatccacaa cgataacctt ttcatgatag aaacttagtt tttctttaaa cttatcttgt
     1321 gttaataata cgcacgtttg actatctttg ataattgttt ctattcgctt actaggatga
     1381 tgaggatcaa ttggtacgta tgcacctcca gcttttaata tcgctagtat ggtcttaacc
     1441 atttcaaacg agcgttccat tgcgacacta acaagaaatt cctcttcacc ttgactcttt
     1501 aattttctta ttttcctcgc aagttgattc gcttgttcat tcaattcttt ataagtcatc
     1561 gttcgtccag caaactggag tgctaccttg tctggtattc gctctacttg tttctcaaat
     1621 aattcatgta ataatgtatt attatgattg tcatattgag tttgattgaa atcatgaagg
     1681 agtaacttct cttcttcacg atttatcatc tctatttccg ctaactttat attagatgat
     1741 tcagaaattt tttctaaaat ggctaaaaaa tgttcactca atctctcaat actatctttc
     1801 ttaaagagct ctgtactata ttcgaattgt gcatgtaagc cctcttccac ttcggttata
     1861 tataaggaaa gatcgaattt agaagaattt aattcgatat ccaccgttga gaattgcaat
     1921 ccatctatac taatttttga gtaactggaa tcttgaaatg taaacatcgt gtcaaaaata
     1981 ggatttcgac tagtatcacg ctgtactttt aatttttcca ccaacaattc aaacggatag
     2041 tcttggtttt caaatgcatt caatgtagat ttttttactt catccaaaaa ttccaagaat
     2101 gttttatcct ttttagggcg atttcgaagc gcaagagtgt ttacaaacat tccaataaca
     2161 ttttcaacat ctagatgatt tcgcccagaa attggagtac ctacgatgat atcttcttgg
     2221 tcaccatact tcgaaagtaa tacattatat gctgctaaca ataacatgaa aagggtcgta
     2281 tcctttctag cagctatttg atgcatatga cgggttaatg tttttcctat atagacagaa
     2341 atactatccc ctttcgatcc ttttaccgat ggtctaggat aatccgttgg taactgaagt
     2401 acaggatatt cctctgaaaa ttgacttaac caataatgct cctgactttg taattcttca
     2461 gatttcaaaa agttattttg ccacgcagcg taatctttat attgaatatg aagctctggt
     2521 aattcttttc cttcatataa ttgaacaaat tcatttacca ataactgaat agattgccca
     2581 tctgcaataa tgtgatgcat atcaattaac aataagtgtt tgttctcttt aagctctaca
     2641 agttttaatc ttattaacga attattgtat aaatcaaatg gttcaacaaa atttgctatt
     2701 aattcagata tttttttatc ccttagatga agcaactcaa tttcaatagg gttataatca
     2761 tgaatttgtt gaacaggatt accatcctta actttaaaga atgttcttaa tgattcatgc
     2821 cttttcaata acatttggaa agtagattca acccgtttta tatcaagcgt cccttcaata
     2881 atcattgctt ttggcatatt ataactcgtt tgaaatttat ttaatgcctg taacatataa
     2941 attctttttt gcgcagaaga aagcaaataa tcttcccttt cctctacttt ttcaattgca
     3001 tatatctttt cacttttaat ttgagaaatg tatgcagatt gttccttgac agttgatagt
     3061 tgaaaaatga tatgtatagg aatagagatt tcaaattctc gataaattct cgatacgaat
     3121 gaggcaatct ttaatgaatg cattcctaaa ttaaaaagat tatcggatat acctattttt
     3181 tctttttcaa taccaaggat ttgactccat atggtaatca atttttcttc caactcattc
     3241 caaggtgcat gataggttga ttctccactt attgcttgtt ttggttctgg gagagatttt
     3301 ttgtctatct ttccatttat cgttagagga aattgatcaa tcttaacaaa gtaatcggga
     3361 atcataaaac ctgggagtct tttttcaagg aaatttctaa atgttgacac gggtatttct
     3421 tccgatgaca tgtaataagc acataaaaaa ttcgattctt ctgaatcctt aagtgctata
     3481 acaagaacat catcaataga ttgatattct tttaattgat actcaatatc tttaagttca
     3541 atccgaaaac ctctaatttt cacctgttcg tctaatcttc ctagatattc tagattccca
     3601 tcaggcaacc aacgcgcaag atctcccgtt ctgtacatta aactacctga aaaaaagggg
     3661 ttaggtacaa ttttttcatt tgttaagggt tgattattaa catatccttt tgcaagagat
     3721 tcaccggaaa tacatagttc accaggcata ccaataggtt gtaatttctg ttcattgtct
     3781 aatatataaa catagttgtt tgcgattggc ttgcctatgg aaatcttaga atcgaccttt
     3841 aaatttcgat aaacagtagt aacaacacta ttctctgtag gcccatattc attcgcaatt
     3901 tcaacatctt tcaatttttc tgaactttcc ctcaaaagat ttgctgtaac ttcctcacca
     3961 gctaacgtaa taatccttaa acataactga tcttcatatt gtatctcagc caatagactg
     4021 ctataaagcg atggtgtact aataaaatgt gttatattat gacgttgaac aatattatac
     4081 aaatagggtg ggttctttac atcatcattt cctactaata ttatttgtgc acctgaaaca
     4141 agcggtgtaa agaaactcgt tacaaatcca tcaaacgaaa aagagaaaag ctgaagtaca
     4201 cgatccttct ctgtcagctt gtattcattt tttctccaaa gaatgttatt tgtaatactt
     4261 ttatgctgaa tcatgacacc tttcggctta ccagtagttc cagacgtgta aatactatag
     4321 gctaaatctt ctcctttatt gattgaaggt aaattccctt gattttttgt attcttttgt
     4381 aacacttcta aatcaattat ctctccattg aagtctaatt tagttctatc ttccgtttct
     4441 tttaataata gacctaaacc gctgtcttgg ataattgcat tttgacgttc taatggatac
     4501 tttggatcta ttggtacata agcaccacca gcttttaata tacctaaaag cccaatagcc
     4561 atctctatac ccggactaat cattaatcca acgaacgtat ttgaagatac ccctttttga
     4621 cgtaaaagcg cagccaattg attggctctt ttattaagtt cagaataagt aatttcactt
     4681 tcctcaaatc tgattgcaac tctatcagca ctttttttga cttgttcctc taccaattca
     4741 tgtagcagtt ttttattacc aaaattcaca gttgtttggt taaattcctt aagtaatttt
     4801 tccttttctc tctctgtaac taaatctaaa tcgccgactt tatattctgg gttatctatg
     4861 aattgttcga tggtcatgac aaactgcgta gccagattac gtataaaatc actactatat
     4921 actttctgat aaataatttc tcctgtaatc tgattatcat cattttgaga aaaatgaaac
     4981 gctattttag ctcgtgtatc acttgtacaa tttttttctc gaagattttc aaaattaata
     5041 agtacaggga agtcaatgtc ctctgtctct gaatgaagta atcccatgga tgataataga
     5101 gaactaatat ttaatttctg atgatcaaca gcatcattaa ccgtgttttt tacgtttaat
     5161 agaagttctt tccatgtcat atcaggcgat accgtatttc tgaataaagt aagatcatcc
     5221 ataccgtttt ttaatccggt tgtacctatt aatatatcgt tattccttgt atatttttgc
     5281 agaataacat ttatagacgt aattaaaatt aagaatgctc cccatttcga ataattacta
     5341 atcttattaa tttttttgct tacctctatt ggaattggga tggaaaactt caccgttccc
     5401 tcacttttaa ctgtttctag atctggcaga aaactactaa actcaatatc ccctgataat
     5461 ttttctaccc agtagtgttt ctctttttca agatcttcat ttatgtataa ttctggcgtt
     5521 aacgtgttcg gtttcataac attttccttt cttttgcttc ttagtatggc ctgtaaaagg
     5581 tcatactaag aagtagttaa tattaaaatg agaatgttaa atcctctgga aacgaaatct
     5641 cctcagatgc ttggctacct tctactttta tatcagcgat ttttatcatt gtgtctacgg
     5701 taacttgttt tgcaatcaaa tctatatctt ctgcaatttt ctttatcgtt tcttgtttat
     5761 agatattgtc tttgtatacg atatttagat taaaataatc tccactttcg actacattaa
     5821 cagttagatc aaatttagcc ttaccactct cataaggata ttcttttaac ttcaaacctg
     5881 ctacattata aaaatgatta ttaattttat cctttttata aataaacata gtgttaataa
     5941 tcccttgaga actattcata ttactaaatt gaaggtagtc caacagcatt tcataaggat
     6001 agtcttggtt ttcaaaagcc aacatcgtat tattcttaac ttgttgcaag aagttgataa
     6061 atgttaaatc tgcctttgga ttattacgaa ttgctaacgt atttacaaac ataccaacta
     6121 tattttctac atctgtatgt gttctaccag cagttggagt tccaacaata acatcatcag
     6181 aattggtata tttagagacc aaaattgtat acagggcaaa aaacatttga aacaacgtta
     6241 ttttatttgc ttttgatagg ttaactaaat tctgacaaac ttcttgattt aatttataat
     6301 taagaactct tctttgattt gaatgaggat tattactagg ataatcaacc ggaagcttct
     6361 ttagcagaac atccccttta tacaaattgt gccaatatgt ttcttgcttt tttaattgct
     6421 cctgaaactc gctactttgc tgccaaacag cataatcttt gtattgtagt gaaggttctg
     6481 aaagaatatc ccctttataa agtcgaataa tttcattcaa taataattga agagaaaatc
     6541 cgtcagcaat aatatgatgc acatcaatac aaagaacaaa ttttgattta tcaatttcaa
     6601 aaagtttcgc tcgtattaat ggtgctttcg ttaagtcaaa aggtgttata aacttttcaa
     6661 catttagacg aatatctgct tcattaatca ctttgtattc cagtaaaaaa ggaatatttg
     6721 gatgtatctt ttgtacaaat tctccatcta caaacccaaa cgaagtacga aatgcctcgt
     6781 gtcgctttat aagttcattc aatgtacctt ctaagcgatg tctatccaat tcgccttcaa
     6841 tttccaataa tactggcgta ttatagcttg tccccatttg tttcgtttgt tctaagataa
     6901 agagtctttg ttgtacatat gatagcgaat aatactcttt cttttgagct tttggtatcg
     6961 gcttatgcat aaattcttta ctatttataa ggtgaactgc taaggacttg attgttgaca
     7021 ttttaaatat atctcttaaa ccaatttctt tattaaactg cttatttatt tttgttaata
     7081 atgttacagc atttaaagaa tgaccaccca gctcgaaaaa gtcattggta actccaatac
     7141 gattatcttc aattcccaat acttctgacc atatttgcac caatttagct tccatttcat
     7201 ttcgtggtgc tatgtattcc tcttgattta agataacttc ttctttaggt aatgcctttc
     7261 ggtctaactt accatttaat gataatggca tgttttccaa atgcacaaaa ttagttggaa
     7321 tcatatattc tggaagcttc tctctcatgt attgatgcca ttctttatta ctccattcaa
     7381 catccgacac gatatatgca cataaatatt ttcttccatg aggatcttcc ctatcaataa
     7441 cgactacctc acgaacgcct tcacgtcgca tgatgcaata ctctatctcc ccaatttcaa
     7501 tacgatatcc tcgtacttta atttggaaat cttttctacc taaatactca atattcccat
     7561 ctggtaacca tctgaccaaa tcacctgttt tatataagac ggtatctttt ttgtaaggat
     7621 gggagataaa tttctccttc gttcgtttct catcattgag atatcctctt gctacccctt
     7681 ctccggctat acacaattca ccaggaatgc ctatcggttg taatttccct tcattactta
     7741 atatatacac acctgtatta tcaagtggtt ttccaatcgg taaaattgac atgtcaagat
     7801 tattgtctag cgcataccct gttgtataaa tactcgcttc cgttgggcca tagagattat
     7861 ataaatcagt atgcgataat aacattcttg ccgatttagc caatttgggc gataatgcct
     7921 caccagcaac aaatatatat tttaaatctg ttaattcctg taactgttta tcagggacca
     7981 tgtcgacaaa tgcttgaagc atagaaggta caaaattgac atgtgtcacc cgctcttttt
     8041 gaatcacctt atatagttcc actggatcct tttccatttc aggctctaaa ataaccagcc
     8101 ttcctcctcc aagcatccag ccaaataatt ctgctaccga gacgtcaaat ataaaaggtg
     8161 tttttaataa atatgcatcc tcttctgtaa taggaaattt tctctgtaat ccgaataatg
     8221 tatttaaaac acttcgatgt tcaatcataa cacccttcgg cttacctgta gaacctgagg
     8281 tatagatgac atatgctaaa ttttcaagat tactaacttg acttaaatta gatacttctt
     8341 ctttgtgatc tttttggtca tccacaacga taaccttttc atgatagaaa cttagttttt
     8401 ctttaaactt atcttgtgtt aataatacgc acgtttgact atctttgata attgtttcta
     8461 ttcgcttact aggatgatga ggatcaattg gtacgtatgc acctccagct tttaatatcg
     8521 ctagtatggt cttaaccatt tcaaacgagc gttccattgc gacactaaca agaaattcct
     8581 cttcaccttg actctttaat tttcttattt tcctcgcaag ttgattcgct tgttcattca
     8641 attctttata agtcatcgtt cgtccagcaa actggagtgc taccttgtct ggtattcgct
     8701 ctacttgttt ctcaaataat tcatgtaata atgtattatt atgattgtca tattgagttt
     8761 gattgaaatc atgaaggagt aacttctctt cttcacgatt tatcatctct atttccgcta
     8821 actttatatt agatgattca gaaatttttt ctaaaatggc taaaaaatgt tcactcaatc
     8881 tctcaatact atctttctta aagagctctg tactatattc gaattgtgca tgtaagccct
     8941 cttccacttc ggttatatat aaggaaagat cgaatttaga agaatttaat tcgatatcca
     9001 ccgttgagaa ttgcaatcca tctatactaa tttttgagta actggaatct tgaaatgtaa
     9061 acatcgtgtc aaaaatagga tttcgactag tatcacgctg tacttttaat ttttccacca
     9121 acaattcaaa cggatagtct tggttttcaa atgcattcaa tgtagatttt tttacttcat
     9181 ccaaaaattc caagaatgtt ttatcctttt tagggcgatt tcgaagcgca agagtgttta
     9241 caaacattcc aat
//
