LOCUS       NZ_MOOL01000049        12210 bp    DNA     linear   CON 03-APR-2024
DEFINITION  Bacillus thuringiensis serovar xiaguangiensis strain BGSC 4BN1
            contig000049, whole genome shotgun sequence.
ACCESSION   NZ_MOOL01000049
VERSION     NZ_MOOL01000049.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis serovar xiaguangiensis
  ORGANISM  Bacillus thuringiensis serovar xiaguangiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 12210)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Comparative genomics of Bacillus thuringiensis reveals a path to
            pathogens against multiple invertebrate hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12210)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, 1st Shizishan,
            Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.5.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002147905.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 02:08:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,596
            CDSs (total)                      :: 6,434
            Genes (coding)                    :: 6,198
            CDSs (with protein)               :: 6,198
            Genes (RNA)                       :: 162
            rRNAs                             :: 15, 15, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 1 (5S, 23S)
            partial rRNAs                     :: 15, 12 (16S, 23S)
            tRNAs                             :: 114
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 95 of 236
            Pseudo Genes (incomplete)         :: 153 of 236
            Pseudo Genes (internal stop)      :: 70 of 236
            Pseudo Genes (multiple problems)  :: 63 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:39:27
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 12210
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOOL01000049.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..3492
                     /locus_tag="BK733_RS22485"
                     /old_locus_tag="BK733_21435"
     CDS             <1..3492
                     /NRPS_PKS="Domain: AMP-binding (179-581). E-value:
                     6.2e-112. Score: 366.1. Matches aSDomain:
                     nrpspksdomains_BK733_RS22485_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (687-755). E-value: 1e-16. Score:
                     52.9. Matches aSDomain: nrpspksdomains_BK733_RS22485_PCP.1"
                     /NRPS_PKS="Domain: TD (791-1039). E-value: 1.2e-48. Score:
                     157.5. Matches aSDomain: nrpspksdomains_BK733_RS22485_TD.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: NAD_binding_4"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /locus_tag="BK733_RS22485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_21435"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_140157173.1"
                     /sec_met_domain="AMP-binding (E-value: 1.3e-121, bitscore:
                     395.3, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.9e-15, bitscore:
                     45.5, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 3.3e-08, bitscore:
                     22.2, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 4.1e-08,
                     bitscore: 21.9, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 1.4e-48, bitscore:
                     154.4, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="IGMFVNTLALRNRPKKDKTFLEFLDEVKKSTLNAFENQDYPFELL
                     VEKLKVQRDTSRNPIFDTMFTFQDSSYSKISIDGLQFSTVDIELNSSKFDLSLYITEVE
                     EGLHAQFEYSTELFKKDSIERLSEHFLAILEKISESSNIKLAEIEMINREEEKLLLHDF
                     NQTQYDNHNNTLLHELFEKQVERIPDKVALQFAGRTMTYKELNEQANQLARKIRKLKSQ
                     GEEEFLVSVAMERSFEMVKTILAILKAGGAYVPIDPHHPSKRIETIIKDSQTCVLLTQD
                     KFKEKLSFYHEKVIVVDDQKDHKEEVSNLSQVSNLENLAYVIYTSGSTGKPKGVMIEHR
                     SVLNTLFGLQRKFPITEEDAYLLKTPFIFDVSVAELFGWMLGGGRLVILEPEMEKDPVE
                     LYKVIQKERVTHVNFVPSMLQAFVDMVPDKQLQELTDLKYIFVAGEALSPKLAKSARML
                     LSHTDLYNLYGPTEASIYTTGYALDNNLDMSILPIGKPLDNTGVYILSNEGKLQPIGIP
                     GELCIAGEGVARGYLNDEKRTKEKFISHPYKKDTVLYKTGDLVRWLPDGNIEYLGRKDF
                     QIKVRGYRIEIGEIEYCIMRREGVREVVVIDREDPHGRKYLCAYIVSDVEWSNKEWHQY
                     MREKLPEYMIPTNFVHLENMPLSLNGKLDRKALPKEEVILNQEEYIAPRNEMEAKLVQI
                     WSEVLGIEDNRIGVTNDFFELGGHSISILKALTKMYFHGWNISVKDFYTTPTIRSLVEK
                     IKLVEDSAEVVHHPEIMNINSPNNRQIMPSGNIKTMDNILVTGATGFLGIHIIRELLEE
                     TDATIHCLVRGPVAEERFLNKIRLYFNDTLLENWNDIKHRIKIIAGDIKQDNLGMVKDD
                     YIKLGNTIDTVIHAAAIVKHYGDKDEFNKINIEGTEKMIDFCSNHDIYFHYISTISISG
                     TRTMDQYQRHFTENNLYIKQDIFDNVYLNSKFEAESRIFEAQRNGLQASIYRVGNLTGR
                     FTDGVFQENIEENAFYRKFKFLVDFKAIPDYLLNQIIEFTPVDICSKAIVKILKTEESC
                     GNVFHLFNHNQVKMVDIKAVLDSLGIRINVLQEDKFNTFIDTIYRNNELKDELSTIITE
                     LINVESNQYKWDVHVDSSFTRKYLQQLDNFNYPIIESIYIGKLLKHMKEVGYLKGK"
     source          1..12210
                     /country="China"
                     /db_xref="taxon:180870"
                     /isolation_source="bean"
                     /mol_type="genomic DNA"
                     /organism="Bacillus thuringiensis serovar xiaguangiensis"
                     /serotype="H51"
                     /serovar="xiaguangiensis"
                     /strain="BGSC 4BN1"
                     /submitter_seqid="contig000049"
     region          1..12210
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX) and not alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..12210
                     /SMILES="NC(C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12210
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:3492](+)"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      <1..3492
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     CDS_motif       88..204
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22485_0001"
                     /evalue="1.60E-20"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="BK733_RS22485"
                     /protein_end="67"
                     /protein_start="28"
                     /score="65.5"
                     /tool="antismash"
                     /translation="KSTLNAFENQDYPFELLVEKLKVQRDTSRNPIFDTMFTF"
     aSDomain        541..1746
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS22485_AMP-binding.1"
                     /evalue="6.20E-112"
                     /label="BK733_RS22485_AMP-binding.1"
                     /locus_tag="BK733_RS22485"
                     /protein_end="581"
                     /protein_start="179"
                     /score="366.1"
                     /specificity="substrate consensus: Ala"
                     /tool="antismash"
                     /translation="FEKQVERIPDKVALQFAGRTMTYKELNEQANQLARKIRKLKSQGE
                     EEFLVSVAMERSFEMVKTILAILKAGGAYVPIDPHHPSKRIETIIKDSQTCVLLTQDKF
                     KEKLSFYHEKVIVVDDQKDHKEEVSNLSQVSNLENLAYVIYTSGSTGKPKGVMIEHRSV
                     LNTLFGLQRKFPITEEDAYLLKTPFIFDVSVAELFGWMLGGGRLVILEPEMEKDPVELY
                     KVIQKERVTHVNFVPSMLQAFVDMVPDKQLQELTDLKYIFVAGEALSPKLAKSARMLLS
                     HTDLYNLYGPTEASIYTTGYALDNNLDMSILPIGKPLDNTGVYILSNEGKLQPIGIPGE
                     LCIAGEGVARGYLNDEKRTKEKFISHPYKKDTVLYKTGDLVRWLPDGNIEYLGRKDFQI
                     KVR"
     aSModule        541..3120
                     /complete
                     /domains="nrpspksdomains_BK733_RS22485_AMP-binding.1"
                     /domains="nrpspksdomains_BK733_RS22485_PCP.1"
                     /domains="nrpspksdomains_BK733_RS22485_TD.1"
                     /final_module
                     /locus_tags="BK733_RS22485"
                     /monomer_pairings="Ala -> Ala"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       733..774
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22485_0002"
                     /evalue="2.70E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="BK733_RS22485"
                     /protein_end="257"
                     /protein_start="243"
                     /score="20.3"
                     /tool="antismash"
                     /translation="LAILKAGGAYVPID"
     CDS_motif       961..1020
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22485_0003"
                     /evalue="7.50E-12"
                     /label="NRPS-A_a3"
                     /locus_tag="BK733_RS22485"
                     /protein_end="339"
                     /protein_start="319"
                     /score="36.8"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGKPKGVMIEH"
     CDS_motif       1540..1629
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22485_0004"
                     /evalue="3.20E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="BK733_RS22485"
                     /protein_end="542"
                     /protein_start="512"
                     /score="48.2"
                     /tool="antismash"
                     /translation="PIGIPGELCIAGEGVARGYLNDEKRTKEKF"
     CDS_motif       1711..1776
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22485_0005"
                     /evalue="2.70E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="BK733_RS22485"
                     /protein_end="591"
                     /protein_start="569"
                     /score="35.4"
                     /tool="antismash"
                     /translation="YLGRKDFQIKVRGYRIEIGEIE"
     aSDomain        2065..2268
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS22485_PCP.1"
                     /evalue="1.00E-16"
                     /label="BK733_RS22485_PCP.1"
                     /locus_tag="BK733_RS22485"
                     /protein_end="755"
                     /protein_start="687"
                     /score="52.9"
                     /tool="antismash"
                     /translation="EAKLVQIWSEVLGIEDNRIGVTNDFFELGGHSISILKALTKMYFH
                     GWNISVKDFYTTPTIRSLVEKIK"
     aSDomain        2377..3120
                     /aSDomain="TD"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS22485_TD.1"
                     /evalue="1.20E-48"
                     /label="BK733_RS22485_TD.1"
                     /locus_tag="BK733_RS22485"
                     /protein_end="1039"
                     /protein_start="791"
                     /score="157.5"
                     /tool="antismash"
                     /translation="ILVTGATGFLGIHIIRELLEETDATIHCLVRGPVAEERFLNKIRL
                     YFNDTLLENWNDIKHRIKIIAGDIKQDNLGMVKDDYIKLGNTIDTVIHAAAIVKHYGDK
                     DEFNKINIEGTEKMIDFCSNHDIYFHYISTISISGTRTMDQYQRHFTENNLYIKQDIFD
                     NVYLNSKFEAESRIFEAQRNGLQASIYRVGNLTGRFTDGVFQENIEENAFYRKFKFLVD
                     FKAIPDYLLNQIIEFTPVDICSKAIV"
     gene            3566..4513
                     /locus_tag="BK733_RS22490"
                     /old_locus_tag="BK733_21440"
     CDS             3566..4513
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PPDK_N"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /locus_tag="BK733_RS22490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_21440"
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="WP_176328710.1"
                     /sec_met_domain="PPDK_N (E-value: 1e-97, bitscore: 316.5,
                     seeds: 105, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIIPLREGYSKDRSLVGGKARNLSKLMEAGIKVPNGFIISSKSFW
                     EFIEINNLANQINHYLKTMENPSHSIKELFHVSKIPSDLEMEVKNAYDNLLSSTEVAKV
                     SVRSSAAAEDLHDSSFAGQYETFLNVGSFEGLVKSIKECWASVWSNQALQYLHNVNIDI
                     KQVKIGVLVQSMVQADTSGVVFSVNPVTSLYDEIIINASYGLGEAIVSGLITPDMFIVS
                     KQDNTILYKELGLKEFKVVGTVDGTKEIPTTEQEKALFSLHDNQIEQITEVTKKIENYY
                     QYPVDVEFAINENEMYVLQVRPITTVKERVNFEK"
     gene            4503..6119
                     /locus_tag="BK733_RS22495"
                     /old_locus_tag="BK733_21445"
     CDS             4503..6119
                     /GO_function="GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /locus_tag="BK733_RS22495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_21445"
                     /product="PEP-utilizing enzyme"
                     /protein_id="WP_088021035.1"
                     /transl_table=11
                     /translation="MKNKLIEQDFLLSNDDMDRAFWVQNDVHFPRPLTPLFTSLIMPAM
                     SNGTEVCYEKMKMQIKKVDVKSLNGYYYQSVVPRSKEDYSHEKHKSIMMNEIPNITKKL
                     DDYVDNIFNPFYENLSAFQTKNKSSQLILQAVKELEQFFMQAWVIHYEVLTPKQAAGWI
                     LEEMYSQLFSSEDKSVIYDLLIGTMNKSLETDKAIWELSRKVKSSTKLNYVFEKTDIES
                     LAEQLVSIEEGKEFISDLQRVMNEYGYRSANFHDFNEETWVENPKYILGIVKTYLNINY
                     DFNEKFIELNTKRNKLFNQLLSEIPDSKEKQRFISMIQTTLHIWRIEEDHHFYIDAMLP
                     ARSRLFFLEVGNYLVEQQMILDKHDIFYLYINEVKELLEQPNNVRDLIETRKKKHRENM
                     KNIPVPFVGDPANMGNAYELEQIVGFPQEPINKQQQSFKGAAASKGLYKGKVKIIHSQE
                     DFHRVNKGDILVSKTTTPPWTVLFPIVGAIITDSGGILSHAGIIAREYGVPAVLGTKVA
                     TSILKNGDEVTVDGTSGIVYF"
     gene            6156..6890
                     /locus_tag="BK733_RS22500"
                     /old_locus_tag="BK733_21450"
     CDS             6156..6890
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Thioesterase (4-228). E-value: 6e-41.
                     Score: 133.3. Matches aSDomain:
                     nrpspksdomains_BK733_RS22500_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thioesterase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1004: thioesterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /locus_tag="BK733_RS22500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_21450"
                     /product="thioesterase domain-containing protein"
                     /protein_id="WP_088021036.1"
                     /sec_met_domain="Thioesterase (E-value: 7.4e-43, bitscore:
                     136.6, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQKIRLFCLPYAGGSSYAFGKWNTHLHPSIDLVPIELPGRGRRIN
                     EPLISNYKEMVNDIFETIKNELDDLPYILFGHSMGSLLVYELVYKILESNKKMPISIIF
                     SGAEPPKLRKQKVISTLPDEEFIEEILAIGGTPKNIFSNKELAKIFISILRYDFQLIES
                     YIPGDYNPLDIDVVIFYGRQDDSTSFTNLQEWKNHTTRACNIIAFEGGHFFIHDNEREV
                     VKEINRLGTSSEIYTNIKGECI"
     aSDomain        6168..6839
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS22500_Thioesterase.1"
                     /evalue="6.00E-41"
                     /label="BK733_RS22500_Thioesterase.1"
                     /locus_tag="BK733_RS22500"
                     /protein_end="228"
                     /protein_start="4"
                     /score="133.3"
                     /tool="antismash"
                     /translation="RLFCLPYAGGSSYAFGKWNTHLHPSIDLVPIELPGRGRRINEPLI
                     SNYKEMVNDIFETIKNELDDLPYILFGHSMGSLLVYELVYKILESNKKMPISIIFSGAE
                     PPKLRKQKVISTLPDEEFIEEILAIGGTPKNIFSNKELAKIFISILRYDFQLIESYIPG
                     DYNPLDIDVVIFYGRQDDSTSFTNLQEWKNHTTRACNIIAFEGGHFFIHDNEREVVKEI
                     NR"
     CDS_motif       6366..6416
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS22500_0001"
                     /evalue="1.00E-05"
                     /label="NRPS-te1"
                     /locus_tag="BK733_RS22500"
                     /protein_end="87"
                     /protein_start="70"
                     /score="18.0"
                     /tool="antismash"
                     /translation="PYILFGHSMGSLLVYEL"
     misc_feature    6990..7006
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 24.92"
                     /tool="antismash"
     gene            7515..8201
                     /locus_tag="BK733_RS22505"
                     /old_locus_tag="BK733_21455"
     CDS             7515..8201
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: ACPS (104-167). E-value: 7.8e-15. Score:
                     46.9. Matches aSDomain:
                     nrpspksdomains_BK733_RS22505_ACPS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1012: 4'-phosphopantetheinyl transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /locus_tag="BK733_RS22505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_21455"
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="WP_088021037.1"
                     /transl_table=11
                     /translation="MSQIHAIKVPEKIVDADYEKLVNCVSASKKEKLSRYFNKRDAYRS
                     LLGDLLVRVFIWDKFLIPNDQITLSFNKYEKPEIDLGFPFSFNISHSKDWVVCIFGNQE
                     VGVDIEQMDKVNLDLVKSYFSQTEYEDLIILPEPMRTKYFFNLWTLKESYIKCIGTGLN
                     CPLNSFTIRINGSSISFKAIDNILSPNLSFKLYHFDSSYSLAACANSILPDNVTVITFE
                     ELIGRI"
     misc_feature    7519..7532
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.64"
                     /tool="antismash"
     misc_feature    complement(7530..7543)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.65"
                     /tool="antismash"
     aSDomain        7827..8015
                     /aSDomain="ACPS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS22505_ACPS.1"
                     /evalue="7.80E-15"
                     /label="BK733_RS22505_ACPS.1"
                     /locus_tag="BK733_RS22505"
                     /protein_end="167"
                     /protein_start="104"
                     /score="46.9"
                     /tool="antismash"
                     /translation="VGVDIEQMDKVNLDLVKSYFSQTEYEDLIILPEPMRTKYFFNLWT
                     LKESYIKCIGTGLNCPLN"
     gene            8247..9101
                     /locus_tag="BK733_RS22510"
                     /old_locus_tag="BK733_21460"
     CDS             8247..9101
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="BK733_RS22510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_21460"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_088021038.1"
                     /transl_table=11
                     /translation="MGKLVELKNISKGYTNKNVLRDISLTIDEGEIIAILGGNGTGKST
                     ILRIIAGLERPSSGKVIYPKKSIRVGYVPERFSKLLRFTPSEYLNYVGKIDGLSKEYLT
                     KRIPYLLNRFQLKALNDQWIMNLSKGNIQKVGLIQAILQSPELLILDEPLSGLDLHAQQ
                     ELLTVLKELKEQGTTILLTYHESNIFESLVEKTYDLNNGLISKVNSVKIEQEKIKLLKV
                     KQIDDSHVKQWHEVISMEKKDNNLLLYVHLKNSDVILSRVLQLKGSIDSVSTIDFDRKI
                     TNE"
     gene            9366..10073
                     /locus_tag="BK733_RS22515"
                     /old_locus_tag="BK733_21465"
     CDS             9366..10073
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BK733_RS22515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BK733_21465"
                     /product="hypothetical protein"
                     /protein_id="WP_088021039.1"
                     /transl_table=11
                     /translation="MKELIKYSLQDYIRSHKYFPPISTFFILIVVNYTYKPNPVISSYA
                     ITALILYIISSWMCVSFLSLDSPVQKQIMTLHIKSSNRYYISKLISVWLISIVLAVFAF
                     LYPIIFNMFNEPVTIIVGLVSLVNHIALATLGISVASLFSKWIVDNPVNSYGGLAITIM
                     TSIGAIGIYDLLPSTFKNIVWIIPPAVSTQTPLIHWSGKSIFELSLFPFIWVFIYSVLI
                     LYLFLKLSKRLMR"
     gene            10200..10748
                     /gene="lepB"
                     /locus_tag="BK733_RS22520"
                     /old_locus_tag="BK733_21470"
     CDS             10200..10748
                     /EC_number="3.4.21.89"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /GO_process="GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /gene="lepB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250768.1"
                     /locus_tag="BK733_RS22520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_21470"
                     /product="signal peptidase I"
                     /protein_id="WP_088021040.1"
                     /transl_table=11
                     /translation="MKENAGNEIYSWIKSLALALIIAFIVRQFLFSPMNVIGESMLPTF
                     ENKDKVMVSKLSYLQRFDIVVFDAPDANEYYIKRVIGLPGDSIEMKGGVLYINGKEIEQ
                     PYLEGNKGDNNSSGMRTDDFTLQELTGETKVPEGSLFVLGDNRIISKDSRSFGFISKES
                     IIGEVKFQYYPLERIGIPK"
     gene            10913..>12210
                     /locus_tag="BK733_RS22525"
                     /old_locus_tag="BK733_21475"
     CDS             10913..>12210
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116413.1"
                     /locus_tag="BK733_RS22525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_21475"
                     /product="IS4 family transposase"
                     /protein_id="WP_140158800.1"
                     /transl_table=11
                     /translation="MSVSNELQQFAQGIQSALSPNVLRDLARDVGFVQRTSKYQAKDLV
                     ALCVWVSQNVAMTSLTQLSSCLEASTEVLISPEGLNQRFNKAAVQFLQHLLAELLNQKL
                     TSSMPISYPYTSVFKRIRILDSTAFQLPDIFSSIYPGAGGCSHTAGVKIQLEYDLLSGQ
                     FLHIHTGPGKQHDRTYGSLCAPTVTANDLCIRDLGYFHLKDLQHIQDKKAYYISRIKSN
                     TRIYQKNPNPDYFQDGRIKKGTEFIQIDMEVLMNSLQPGQTYEISDAYVGMTDKVPARV
                     IVHRLTKEQQRKRLHDQTVREKKKGMKYSARSKRLSGINVYMTNTPIDIVPMGQVHDWY
                     SLRWQIEILFKTWKSFFHIHHCKKIKRERLECHLYGQLIAILLCSSTMFQMRQLLLMKK
                     KRELSEYKAIYMIKDYFFLLFQSIQKDTQELSKV"
ORIGIN
        1 attggaatgt ttgtaaacac tcttgcgctt cgaaatcgcc ctaaaaagga taaaacattc
       61 ttggaatttt tggatgaagt aaaaaaatct acattgaatg catttgaaaa ccaagactat
      121 ccgtttgaat tgttggtgga aaaattaaaa gtacagcgtg atactagtcg aaatcctatt
      181 tttgacacga tgtttacatt tcaagattcc agttactcaa aaattagtat agatggattg
      241 caattctcaa cggtggatat cgaattaaat tcttctaaat tcgatctttc cttatatata
      301 accgaagtgg aagagggctt acatgcacaa ttcgaatata gtacagagct ctttaagaaa
      361 gatagtattg agagattgag tgaacatttt ttagccattt tagaaaaaat ttctgaatca
      421 tctaatataa agttagcgga aatagagatg ataaatcgtg aagaagagaa gttactcctt
      481 catgatttca atcaaactca atatgacaat cataataata cattattaca tgaattattt
      541 gagaaacaag tagagcgaat accagacaag gtagcactcc agtttgctgg acgaacgatg
      601 acttataaag aattgaatga acaagcgaat caacttgcga ggaaaataag aaaattaaag
      661 agtcaaggtg aagaggaatt tcttgttagt gtcgcaatgg aacgctcgtt tgaaatggtt
      721 aagaccatac tagcgatatt aaaagctgga ggtgcatacg taccaattga tcctcatcat
      781 cctagtaagc gaatagaaac aattatcaaa gatagtcaaa cgtgcgtatt attaacacaa
      841 gataagttta aagaaaaact aagtttctat catgaaaagg ttatcgttgt ggatgaccaa
      901 aaagatcaca aagaagaagt atctaattta agtcaagtta gtaatcttga aaatttagca
      961 tatgtcatct atacctcagg ttctacaggt aagccgaagg gtgttatgat tgaacatcga
     1021 agtgttttaa atacattatt cggattacag agaaaatttc ctattacaga agaggatgca
     1081 tatttattaa aaacaccttt tatatttgac gtctcggtag cagaattatt tggctggatg
     1141 cttggaggag gaaggctggt tattttagag cctgaaatgg aaaaggatcc agtggaacta
     1201 tataaggtga ttcaaaaaga gcgggtgaca catgtcaatt ttgtaccttc tatgcttcaa
     1261 gcatttgtcg acatggtccc tgataaacag ttacaggaat taacagattt aaaatatata
     1321 tttgttgctg gtgaggcatt atcgcccaaa ttggctaaat cggcaagaat gttattatcg
     1381 catactgatt tatataatct ctatggccca acggaagcga gtatttatac aacagggtat
     1441 gcgctagaca ataatcttga catgtcaatt ttaccgattg gaaaaccact tgataataca
     1501 ggtgtgtata tattaagtaa tgaagggaaa ttacaaccga taggcattcc tggtgaattg
     1561 tgtatagccg gagaaggggt agcaagagga tatctcaatg atgagaaacg aacgaaggag
     1621 aaatttatct cccatcctta caaaaaagat accgtcttat ataaaacagg tgatttggtc
     1681 agatggttac cagatgggaa tattgagtat ttaggtagaa aagatttcca aattaaagta
     1741 cgaggatatc gtattgaaat tggggagata gagtattgca tcatgcgacg tgaaggcgtt
     1801 cgtgaggtag tcgttattga tagggaagat cctcatggaa gaaaatattt atgtgcatat
     1861 atcgtgtcgg atgttgaatg gagtaataaa gaatggcatc aatacatgag agagaagctt
     1921 ccagaatata tgattccaac taattttgtg catttggaaa acatgccatt atcattaaat
     1981 ggtaagttag accgaaaggc attacctaaa gaagaagtta tcttaaatca agaggaatac
     2041 atagcaccac gaaatgaaat ggaagctaaa ttggtgcaaa tatggtcaga agtattggga
     2101 attgaagata atcgtattgg agttaccaat gactttttcg agctgggtgg tcattccata
     2161 tcaattttga aagctttaac taaaatgtat tttcatggct ggaatattag tgttaaagat
     2221 ttttacacaa cgccgacaat tcgaagttta gttgaaaaaa ttaagttagt agaagattct
     2281 gctgaggtag tacatcatcc tgaaataatg aatataaatt cacctaataa taggcaaatt
     2341 atgcctagcg gaaatataaa aacaatggat aatattttgg taacaggtgc tacgggattt
     2401 ctagggattc atatcattag agagttacta gaggaaacgg atgcaacaat ccactgtttg
     2461 gtacgaggac cggttgcaga agaacgcttt ttaaataaga taaggttata ttttaatgat
     2521 acattattgg aaaactggaa tgatatcaaa catagaataa aaattatcgc aggtgatata
     2581 aagcaagata atttagggat ggtaaaagat gactatataa aacttggtaa tacaatagat
     2641 acagtaattc atgctgctgc tattgttaaa cactatggcg ataaagatga gtttaacaaa
     2701 ataaatattg agggaacgga aaaaatgata gatttctgtt ctaatcatga tatttatttt
     2761 cactatattt ctacaattag tatttcgggc actcgaacga tggatcaata tcaaaggcat
     2821 ttcacggaaa ataacctcta tattaaacaa gatatttttg acaatgttta tcttaatagt
     2881 aaatttgaag ctgaatcacg aatatttgaa gcgcagagaa atgggttaca agctagtatt
     2941 tatcgggttg gtaatttaac tggaagattt actgatgggg ttttccaaga gaatatagaa
     3001 gaaaatgcgt tttatcgtaa atttaaattt ttagttgatt ttaaagcgat accagattat
     3061 cttctaaacc aaataattga atttacgcct gtagatatat gtagtaaagc tattgttaaa
     3121 attttaaaaa cagaggaatc atgtggtaat gtttttcatc tgtttaatca caatcaagta
     3181 aaaatggttg atatcaaagc agtgttagat tcactaggaa ttaggattaa cgtgcttcaa
     3241 gaagataaat ttaatacatt tatcgataca atatatcgaa acaatgaact aaaggatgaa
     3301 ttatcaacaa ttattactga attaataaac gtggaatcta atcagtataa atgggatgtt
     3361 catgttgatt catcttttac tagaaagtat ttacaacagt tggataattt taactatcca
     3421 attattgaat caatttatat tggaaaattg cttaaacata tgaaagaagt tggatatctt
     3481 aaggggaaat aaaagatttt attagatgga aaggatggga aataagcatg aaattcccat
     3541 cctaactttg aagggagagt tctatatgat tattccttta agagaaggat atagtaaaga
     3601 tcgatcacta gtaggtggaa aggcaagaaa tctatcgaaa ttaatggagg ctggaataaa
     3661 agtaccaaat ggttttatta tatcctccaa atccttttgg gaatttattg aaatcaataa
     3721 tctagctaat caaattaatc actatttaaa aacgatggaa aatcctagtc atagtattaa
     3781 agaattgttt catgttagca aaattccaag tgacttagaa atggaggtaa aaaatgcata
     3841 cgataacttg ctttcttcaa cagaagtagc aaaagtttcg gtaagatctt ctgcagctgc
     3901 agaagattta catgattcat cttttgccgg acaatatgaa acatttctaa acgttggaag
     3961 ttttgaaggt ttagtgaaga gtataaagga atgctgggcc tccgtatggt ctaatcaagc
     4021 tcttcaatat ttacataatg tcaatattga cataaaacag gttaaaattg gtgttttggt
     4081 acaatcgatg gtacaggcag atacatctgg cgtggtattt agtgtgaatc cagtaacctc
     4141 gttatatgat gaaatcatta ttaatgccag ttatggtcta ggtgaggcaa ttgtatcagg
     4201 tctcataacc ccagatatgt ttattgtttc aaaacaagat aataccattt tgtataagga
     4261 gttaggactt aaagaattta aggttgtggg aacagtggat ggtacaaagg aaataccaac
     4321 gactgagcaa gaaaaagcac tatttagttt gcatgataat caaatagaac agattacgga
     4381 ggtaacgaag aaaatagaaa attattatca atatccagtt gatgttgaat ttgcaataaa
     4441 tgaaaatgaa atgtatgtat tacaagtaag gcctataact actgtaaagg agcgtgtcaa
     4501 ctttgaaaaa taaactaata gaacaagatt ttctgttgtc taatgatgat atggacagag
     4561 cattctgggt acaaaatgat gtccattttc ccagaccatt aacaccactt tttacatccc
     4621 ttattatgcc tgcaatgagt aatggtacag aggtatgtta tgaaaaaatg aagatgcaaa
     4681 taaaaaaagt tgatgtaaaa tccttaaatg gctactatta tcagtcggtt gttcctagaa
     4741 gcaaagaaga ttatagtcat gaaaaacata aatcgattat gatgaacgaa attccaaata
     4801 ttacaaaaaa attagatgat tatgttgata atatatttaa tcctttttat gagaatttat
     4861 cagcatttca aaccaaaaat aaatccagtc aattaattct tcaggctgta aaagagttag
     4921 aacaattttt tatgcaagct tgggttatcc attatgaggt actaactcct aaacaagcag
     4981 ctggttggat tttggaagag atgtatagcc aattgttcag ctctgaagat aagtcggtta
     5041 tttatgattt attaattgga acaatgaata aatcattaga gaccgataaa gctatttggg
     5101 aattatcaag gaaagtaaag agttcaacta aattaaatta tgtatttgaa aagacagata
     5161 tcgaaagtct tgctgaacaa ttagtaagta tagaagaggg taaggaattt atttcagatt
     5221 tacaacgtgt catgaatgaa tatgggtata gaagtgctaa ttttcatgat tttaatgaag
     5281 aaacttgggt tgaaaatcct aagtatatcc ttgggattgt aaaaacatat ttaaatataa
     5341 attatgactt taatgagaag tttatcgaat taaatacaaa aagaaataaa ttatttaatc
     5401 aattattatc tgagatacct gatagtaaag aaaaacaaag gtttattagc atgattcaaa
     5461 caactttaca tatttggcgt attgaagaag atcatcattt ctatattgat gctatgcttc
     5521 cagctagatc aagactcttc tttcttgaag ttggtaacta tttagtagaa caacagatga
     5581 ttttagataa gcatgatatt ttttatctat atataaatga agtcaaagaa ttgttagaac
     5641 aacccaataa tgtgagagat ctaatcgaga cacgaaagaa aaagcatcga gagaacatga
     5701 agaatattcc agttcctttt gtaggagacc ctgcaaatat gggtaatgcg tatgagttag
     5761 agcaaatcgt aggtttccca caagagccta taaacaaaca acaacaatct tttaaaggtg
     5821 ccgctgcatc taaaggatta tacaaaggta aggttaaaat cattcattca caggaggatt
     5881 ttcatagggt taataaagga gatatcttag taagtaaaac gacgacacca ccttggacgg
     5941 tcctctttcc aatagtcggt gcaattataa cggatagtgg cggaattctc tctcatgctg
     6001 gaattattgc aagagaatat ggtgttccag ctgtgttggg aacaaaagta gctacttcta
     6061 ttttaaaaaa tggagatgaa gtaactgtag atggtactag tggaatagtt tatttttaat
     6121 tgttagtact acaaacaata ttaaggggtg aatgtatgca gaaaataaga ctcttttgtt
     6181 taccatacgc tggtggatca tcttatgcat ttggtaaatg gaatacccat ttacatccat
     6241 cgattgattt agtaccaata gaattaccag gtagggggag aaggataaat gaacctctaa
     6301 tttctaatta taaagaaatg gtgaatgaca tatttgagac aatcaaaaat gaactagatg
     6361 atttgccgta catattattt ggtcatagta tgggtagtct attagtttat gaattagtat
     6421 ataagatatt agaatcgaat aagaaaatgc caatttcaat tattttctct ggggcagaac
     6481 cgccaaagtt acgaaagcag aaagtaattt ctacgttgcc cgatgaggaa ttcatagaag
     6541 aaattcttgc gataggtggt acaccaaaaa acattttttc taataaagag ttagctaaaa
     6601 tttttatttc tatattaaga tatgattttc aattaattga gtcctatatt ccaggagatt
     6661 ataacccact agatatagat gttgtaatat tttacggaag gcaagatgat tctactagtt
     6721 ttaccaacct acaagagtgg aaaaatcata caacaagagc atgtaatatt atcgcttttg
     6781 aaggtgggca tttttttata cacgataacg aaagagaagt agtaaaagaa attaatagat
     6841 tagggacaag ttcagaaata tatacgaata taaaaggcga atgcatatga atttactgaa
     6901 gttcatcctg aggagataaa tattaataga atgatttatc atgcaaatgg agcttagcat
     6961 cttgtgtgta gttggtattg tcacactaat tgtaattgta ttaaaaaact caatattatt
     7021 gattgatcga attaacttat aaaagaaggt tattctattc aaggtgcatt agtgaaagca
     7081 agaaaaaata gaatgagacc aatttttatg ataactatta taatagtagt aggaatagga
     7141 tctttagcct tagaatctag aatgttcagt ggttattaaa taccattggt aaccgtaatt
     7201 atttggaatt cttattttct acttttacaa cattatagtt aagtcaatcg tttgttaatt
     7261 atttttcttc ttacgtaaga aatcagtaac aaacgtggaa gatacaaaga aattagctga
     7321 ctaacaggaa agggttagtt cttagcttaa tttcgaaatc ttttatgtaa agtcttatga
     7381 caatgctcta atgatgatag cttctgcagg actatatcat tagaggagtg aataacatca
     7441 gtaggaggta aagcctactg atgaatgttt cattttatgg aaactgttag ttttcattga
     7501 attggagtgg tttaatgtcg caaattcatg caataaaggt gccagagaaa atagtagatg
     7561 ctgattatga aaaattggtt aattgtgttt cagctagtaa aaaagaaaaa ttaagtagat
     7621 attttaataa gagagatgca tatagaagtc tacttggtga tttattagtt cgtgtattta
     7681 tatgggataa attccttatt cctaatgatc aaattacgct ttcctttaat aaatatgaaa
     7741 agccagaaat cgatttgggt tttccgttct catttaatat atcccattct aaagactggg
     7801 tagtttgtat atttggtaat caagaagtag gagtggacat tgaacaaatg gataaggtaa
     7861 acctggatct tgttaaaagt tattttagcc aaactgagta tgaagatctt atcattttgc
     7921 ctgaaccaat gagaacgaag tactttttca atttatggac tttaaaggaa agttatatta
     7981 aatgtattgg tactggatta aactgtccat taaattcttt tacgataagg ataaatggat
     8041 cttctatctc atttaaggca attgataata ttttaagtcc aaatttatca tttaagttat
     8101 atcattttga ttccagttat agtttggctg cttgtgcaaa ttcaatatta ccggacaatg
     8161 taaccgtcat tactttcgag gaacttattg gtagaatcta atacatatta ataaatcatt
     8221 gttatttatt aagaaagggg tattttatgg ggaaacttgt tgaacttaag aatattagta
     8281 aagggtacac aaataaaaac gtcctacgtg atatttcgct gacaatcgat gagggggaaa
     8341 ttattgcaat tttaggagga aatggtactg gtaaaagcac catattacgg attatagctg
     8401 gtctagaacg ccctagttca ggaaaagtta tttatcctaa aaaaagtatt agggttggat
     8461 atgtaccaga aagattttct aaacttcttc gttttacccc aagtgaatat ttaaattatg
     8521 tagggaaaat tgatggcctc tctaaagagt atttaacaaa aagaattcct tatttactaa
     8581 atcgttttca acttaaggca ttaaatgatc aatggattat gaatttgtca aaaggtaaca
     8641 tccaaaaggt gggactaatt caggctattt tgcaaagtcc tgaactatta attttagacg
     8701 agcctctgtc cggtcttgat ttacatgcac agcaagagtt attgacagta ctaaaggaat
     8761 taaaagaaca gggtactacc attttattga cataccatga atctaatatt tttgagagct
     8821 tagtagaaaa gacttatgat ttaaataatg gtcttatttc taaggtgaat tctgtaaaaa
     8881 tagaacaaga aaagattaag ttattgaagg taaaacaaat agatgattcc catgttaaac
     8941 aatggcatga agtcatttct atggaaaaga aggataataa tttgttactt tacgttcatt
     9001 taaaaaatag tgatgtcata ctatcaagag tccttcaatt aaaaggaagt attgatagtg
     9061 tatcaactat agacttcgat aggaagatta ctaatgagtg agatgtttga agcgtcttcc
     9121 ttttaatttt tttgaataac cttttactaa caatgaccta tgttttctgt aaaataaaaa
     9181 tgcagtgcaa tatcagtcgg atatggaatt tctacaagca taactcacac actagagacg
     9241 ctgtaatgac aagcatttgg tgctatcatg tttgtctctc catgtaaatc tgtgttatta
     9301 caattctatg tatttttatt ttgaactaaa tatgatctat acataatata aaaggagaga
     9361 ttgttttgaa agaacttatt aaatatagtt tacaagatta tatccgttcc cataaatatt
     9421 ttccacctat ttcaactttc ttcatattaa ttgttgttaa ttatacatac aaacctaacc
     9481 cagtcattag tagttatgca ataactgcat tgattttata tattatatct tcttggatgt
     9541 gtgtaagttt tttatccctt gattcacctg ttcagaaaca aataatgact ctacatatca
     9601 aaagtagtaa tcgttactat atttctaaat taatttcagt atggcttatt tcaattgtac
     9661 ttgctgtttt tgcatttcta tatccaatta tttttaatat gttcaatgaa cctgtaacta
     9721 taattgttgg cttagtttct ttagtaaatc atattgcttt agcaacgtta ggtattagtg
     9781 ttgcgagctt atttagtaag tggatagtag ataatcctgt gaattcttat ggagggctag
     9841 caattactat aatgacttca ataggagcaa tagggattta tgatctttta ccttccacct
     9901 ttaagaatat agtttggatc attccccctg cagtgagcac acaaacgcca ctgattcatt
     9961 ggagtggtaa aagtatcttc gagctttctc tttttccttt tatttgggta tttatttact
    10021 cagtcttaat tttatatcta tttttaaaat tatcgaagcg attaatgcga taaaatttat
    10081 aggatataat cttaatataa aaattcttac cttccataaa aatgacttta ttttctaaaa
    10141 agcaagaaat aaagttgtaa aatattaata ttatttacaa ataaggggtt gaaaaatata
    10201 tgaaagagaa tgcgggaaat gagatctatt cttggattaa atcattagca ttggcattaa
    10261 ttatagcttt tattgttcgt caatttttat tctcacctat gaacgtaatt ggagaatcaa
    10321 tgttacctac ttttgaaaat aaggataaag ttatggtaag taaattatca tatttacaac
    10381 gatttgatat tgtagtattc gacgcaccgg atgcaaatga gtattatatt aaaagggtta
    10441 ttggtctacc tggggatagt attgaaatga aaggcggagt actatatatt aatggaaaag
    10501 aaatagaaca accttatttg gagggaaata agggagataa taattcatct ggtatgcgta
    10561 cagatgactt cactctacaa gaattaactg gtgaaacaaa agtacctgaa ggatcattat
    10621 ttgtattagg ggataatcgt ataattagta aggatagtcg ttcttttgga tttatttcta
    10681 aggaatccat tataggagaa gttaaatttc aatattatcc attggagaga ataggtatac
    10741 caaaataaat cagagtgttg ggaaatactt tgggtttccc aacactctgg tagtcattta
    10801 ttttaatatg gggtaccgcc acatcgctat caagctaaca aaataaaatc cccctaaaat
    10861 gaaaaaatac gctattcttt ctgtagaatc ataggaaagg atggcgtttt ttatgtctgt
    10921 ttctaacgaa ttacaacaat ttgctcaagg gattcaaagt gccctatctc caaatgtctt
    10981 acgggatctt gctagagatg ttggctttgt acaaagaacc agtaagtacc aagcaaaaga
    11041 tttagtcgct ttatgtgtat gggtgagcca aaatgtagct atgacttctt taactcagtt
    11101 atctagctgt ttagaagcat caacagaagt actcatcagc cctgagggct tgaatcaacg
    11161 atttaataaa gcggctgttc aatttttaca acacctatta gctgaactac taaaccaaaa
    11221 actgacctca tctatgccaa tttcttatcc atatacttct gttttcaagc gtattcgtat
    11281 tttagattca actgcatttc aacttccgga tatattttca tcaatttatc ctggtgcggg
    11341 aggatgcagc catacagcgg gagtaaagat acagcttgag tatgatttat taagtggaca
    11401 attccttcat attcatacag gtccaggtaa acaacatgat cgaacctacg gttccctgtg
    11461 tgccccaact gtaacagcga atgatttatg tatccgagac ttaggttatt ttcatttgaa
    11521 agatcttcaa catatacaag ataaaaaggc ttattatatc tcacgtatca aatcgaatac
    11581 acgtatttat caaaaaaatc caaaccctga ttattttcaa gatggcagaa ttaagaaggg
    11641 gacagagttt atccaaatag atatggaggt tttaatgaat tctcttcagc caggacaaac
    11701 atatgaaata tccgacgctt atgtgggaat gactgataaa gtaccagctc gtgtgattgt
    11761 tcatcgacta acaaaagaac aacaacgaaa acgattacat gatcaaacgg taagagaaaa
    11821 aaagaaagga atgaagtatt ctgctcgtag taaacgacta agcggtatca atgtatatat
    11881 gacaaatacc cctatagata ttgtcccgat gggacaagta catgattggt attctttacg
    11941 ttggcaaatc gagattttat ttaaaacatg gaaatcattc tttcatattc atcattgtaa
    12001 aaagataaaa cgagaacgat tggaatgcca tttgtatggg caactgattg ccattctact
    12061 ttgttcttct accatgtttc aaatgcggca attacttctt atgaaaaaga aacgagaact
    12121 gagtgaatat aaggccatat atatgattaa agattacttt tttcttcttt tccaatcgat
    12181 acaaaaagac acccaagagc tatcaaaggt
//
