LOCUS       NZ_MOOL01000024        25239 bp    DNA     linear   CON 03-APR-2024
DEFINITION  Bacillus thuringiensis serovar xiaguangiensis strain BGSC 4BN1
            contig000024, whole genome shotgun sequence.
ACCESSION   NZ_MOOL01000024
VERSION     NZ_MOOL01000024.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis serovar xiaguangiensis
  ORGANISM  Bacillus thuringiensis serovar xiaguangiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 145281)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Comparative genomics of Bacillus thuringiensis reveals a path to
            pathogens against multiple invertebrate hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 145281)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, 1st Shizishan,
            Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.5.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002147905.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 02:08:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,596
            CDSs (total)                      :: 6,434
            Genes (coding)                    :: 6,198
            CDSs (with protein)               :: 6,198
            Genes (RNA)                       :: 162
            rRNAs                             :: 15, 15, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 1 (5S, 23S)
            partial rRNAs                     :: 15, 12 (16S, 23S)
            tRNAs                             :: 114
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 95 of 236
            Pseudo Genes (incomplete)         :: 153 of 236
            Pseudo Genes (internal stop)      :: 70 of 236
            Pseudo Genes (multiple problems)  :: 63 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:39:27
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 93191
            Orig. end                         :: 118430
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOOL01000024.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..25239
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="betalactone"
                     /region_number="2"
                     /rules="(HMGL-like and AMP-binding)"
                     /tool="antismash"
     cand_cluster    1..25239
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(HMGL-like and AMP-binding)"
                     /kind="single"
                     /product="betalactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..25239
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:15239](-)"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..15239)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
     gene            complement(430..1167)
                     /gene="mtnN"
                     /locus_tag="BK733_RS07210"
                     /old_locus_tag="BK733_06855"
     CDS             complement(430..1167)
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /GO_function="GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA]"
                     /GO_function="GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /gene="mtnN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016129475.1"
                     /locus_tag="BK733_RS07210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06855"
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="WP_000151939.1"
                     /transl_table=11
                     /translation="MTIGEVKRVMKRIAIVSAWEPELTYLHQHYPSERVEKRAAWEFHF
                     HTINELEIISVVTGVGKVSCASCVQLLISEFQPDELFMTGICGSLSNKVKNGHIVVALN
                     AIQHDVTAAGSGEDVFNLYNGRTAPIETTKSLVRRIKKIRSYDPIHFGTFLSGDQRIRS
                     SEMRYLLHTVYGALAVDQEVAAFAYVCQINKKPFLCLKAASDQANDKTKEEQKIFKMLA
                     CERACEHLIAFLRVYEINVVNNR"
     gene            complement(1164..1838)
                     /locus_tag="BK733_RS07220"
                     /old_locus_tag="BK733_06860"
     CDS             complement(1164..1838)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002156408.1"
                     /locus_tag="BK733_RS07220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06860"
                     /product="DUF3885 domain-containing protein"
                     /protein_id="WP_043924663.1"
                     /transl_table=11
                     /translation="MQLKEYMNQTFPGVTLGPYLYSQWGNHLHFDFGKDKYQIVEGTND
                     LNMEYFSQLYTYNKFLFEDIFSKEDVVFVVTNVYRFKKENIKTPQKINVYNKYIKKRDL
                     RFHIRQETLPFLFEDEEADLYCTYHFSLKCLAEDIKYELLIQAANHEDFSALYPRFGRK
                     KEISYPDVFLINATKDIVMFMCDDRGCEVIAKNKEIIRDLYKKYKEWIPDYERESIDNL
                     FK"
     gene            complement(1852..2460)
                     /gene="yqeK"
                     /locus_tag="BK733_RS07225"
                     /old_locus_tag="BK733_06865"
     CDS             complement(1852..2460)
                     /EC_number="3.6.1.41"
                     /codon_start=1
                     /gene="yqeK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124593.1"
                     /locus_tag="BK733_RS07225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06865"
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="WP_063547343.1"
                     /transl_table=11
                     /translation="MLCNDIYLFTPTGKIDNDIKAFLLKYNKEFTYKHSIRVANEAKKI
                     AEKFHVDKEKAAIAGYLHDISGIFPNEERIAVAEEFGVEIVETERKIPMIIHQKLSRVI
                     AKEIFKVEDEEILNAICCHTTLRKHATKMDLVLFVADKIEWDQNGTPPYLIEIKKGLEK
                     SLEKAAFVYISYLWERKDTLKVIHPWLEEAYWDLKGILQ"
     gene            2653..3348
                     /locus_tag="BK733_RS07230"
                     /old_locus_tag="BK733_06870"
     CDS             2653..3348
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="resistance (resist) vanR"
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038809.1"
                     /locus_tag="BK733_RS07230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06870"
                     /product="response regulator transcription factor"
                     /protein_id="WP_001038809.1"
                     /transl_table=11
                     /translation="MNKNILIIDDDKEIVELLAVYLRNEGYNIYKAYDGDEALQKVSTY
                     EVDLMILDIMMPKRNGLEVCQEVRENNTVPILMLSAKAEDMDKILGLMTGADDYMIKPF
                     NPLELVARVKALLRRSSFQNATSQKNEDGIIRIRSVEIHKHNHTVKVNGEYIKLTSIEF
                     DILYLLASNTGRVFSSEEIFERVWNEDGYGSNKTVMVHISNLRDKLETVMNGEKLIHTV
                     WGVGYKIEK"
     gene            3338..4495
                     /locus_tag="BK733_RS07235"
                     /old_locus_tag="BK733_06875"
     CDS             3338..4495
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="resistance (resist) vanS"
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016129473.1"
                     /locus_tag="BK733_RS07235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06875"
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_001243312.1"
                     /transl_table=11
                     /translation="MRNEAFDILKDIPKWKLIFWLLLAITLTPITELIIYRLVTSVIES
                     SLTLTELGKEFIRIFGYEKYKGIKESLWMMIVSYLFLFSIFSSYLYIFYRHEKKLYYET
                     CIKKMIEEIRYIANGNFNHKVSIVNHNYLEELAIGVNEIVEQLKVSIDEERQAEQAKSE
                     LITNVSHDLRTPLTSIVGYVNLIHHDNYRDEVELRYYIQVIYDKVTRLNALMNDLFEYT
                     RVQNKELSLYSVPIDIVELLGQLTVQFRIQVQEANIECRPSFPSQKLMVLADGDKLVRV
                     FENLIINAIAYGSEGRYIDLTAYESNNMITIDITNYGQPIPSTDLPHIFERFYRVEKSR
                     STNTGGSGLGLAIAKSIVELHKGTIEVYSDDEKTTFTVKLLPYKC"
     gene            4671..5687
                     /locus_tag="BK733_RS07240"
                     /old_locus_tag="BK733_06880"
     CDS             4671..5687
                     /EC_number="3.4.-.-"
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160038.1"
                     /locus_tag="BK733_RS07240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06880"
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="WP_063547342.1"
                     /transl_table=11
                     /translation="MQFLKKFAILLLSFFITALIVLYFYLYVNGPIIQAKSAILINANS
                     GEIVYKKNEETPVQSATLSKLMTEYIVLEQLNDRKIQLDDFVQISNEVFRVETSPIQVT
                     SNDKTTVRDLLHALLLAGNNRSALALAEHIAGTEDNFTILMNKKAKELQLLQPSPFLNS
                     TGINHDTNKQSTTTAIDAAKLAARLVKDFPDVLNITKLTSYQFTFKDSQVFNTNKMIYS
                     LNENIKLQGVDGLQTSFSTNGNYSFVSTAKLGDTRLISVILDADEENISFIETKKLLQY
                     GFDPSSYSALQAFKDTLTSWTILLQFKNLIIQTIMIFLIITTLMFLHIRQKKSEDFN"
     gene            complement(5749..>5859)
                     /locus_tag="BK733_RS07245"
                     /pseudo=""
     CDS             complement(5749..>5859)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288863.1"
                     /locus_tag="BK733_RS07245"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DinB family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="VLRHVIVHEIHHIGQLSIWARELNLQPVSANLIGRG"
     misc_feature    5863..5872
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            6025..6162
                     /locus_tag="BK733_RS07250"
                     /old_locus_tag="BK733_06885"
     CDS             6025..6162
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376553.1"
                     /locus_tag="BK733_RS07250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06885"
                     /product="DUF3956 domain-containing protein"
                     /protein_id="WP_000376554.1"
                     /transl_table=11
                     /translation="MDSCVVFVNGQPFLVLSVAGIEIARLEISLQVALALRVLGIPICD
                     "
     misc_feature    complement(6253..6269)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.65"
                     /tool="antismash"
     misc_feature    complement(6709..6725)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.51"
                     /tool="antismash"
     gene            6876..7484
                     /locus_tag="BK733_RS07255"
                     /old_locus_tag="BK733_06890"
     CDS             6876..7484
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807974.1"
                     /locus_tag="BK733_RS07255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06890"
                     /product="hypothetical protein"
                     /protein_id="WP_088020314.1"
                     /transl_table=11
                     /translation="MTEIKVGLTQFLDFTLKGSTAKTTFIKNLKSQPEYQPAFDYWKQL
                     RETVIKFHQNRLPFEYFETLVQTVDQKKKQNYIDVIKQYKKFIKNKDISWFDPGKSHWK
                     SDDLIVRSSPELGLFINNEPHLIKLFFKGKKERIDKYNINSTLTLLNESTFSNERNDVN
                     YTVLNIQKNRMYTNNSINNNHLVALKSEANQFCYIWNNL"
     gene            complement(7754..8287)
                     /locus_tag="BK733_RS07260"
                     /old_locus_tag="BK733_06895"
     CDS             complement(7754..8287)
                     /EC_number="2.-.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002007691.1"
                     /locus_tag="BK733_RS07260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06895"
                     /product="GNAT family protein"
                     /protein_id="WP_000990089.1"
                     /transl_table=11
                     /translation="MMYERSMEVIKLKTFKKSDFKQLINWINSEEFLIQWSGNAFTFPL
                     DEQQLEKYIESANTLAFKVVDEETSDVIGHISLGQIDNINKSARIGKVLVGNTKMRGRS
                     IGKHMMKAVLHIAFDELKLHRVTLGVYDFNISAISCYEKIGFVKEGLLRESKRVGETYW
                     NLWEMSMLEYEWKK"
     misc_feature    complement(8363..8378)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 18.06"
                     /tool="antismash"
     gene            complement(8393..8749)
                     /locus_tag="BK733_RS07265"
                     /old_locus_tag="BK733_06900"
     CDS             complement(8393..8749)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535569.1"
                     /locus_tag="BK733_RS07265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06900"
                     /product="hypothetical protein"
                     /protein_id="WP_000535577.1"
                     /transl_table=11
                     /translation="MGSRFNNCKKKCTCKQDDCWEVFEECKKEQHEKCDNSCVQGIRDE
                     LKKLINRTVQINTEGRTYVGTVSSVSCDVVRLAAIPGSVGVIISICKIEAIFPPVTTVT
                     ADFDFNGIDVANKA"
     gene            9347..9679
                     /locus_tag="BK733_RS07270"
                     /old_locus_tag="BK733_06905"
     CDS             9347..9679
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BK733_RS07270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BK733_06905"
                     /product="hypothetical protein"
                     /protein_id="WP_063547340.1"
                     /transl_table=11
                     /translation="MIAGVLRKSIVVMIGGNLKKSIIVMMIGVLRKSIVMMIGGNLKKS
                     VIVMMIGVLNEISVVVNVSLFVAIVKEKIGNIFNVNVKKKICLLESIGLFRKVDPSPTS
                     LLSLNF"
     gene            complement(10001..11941)
                     /locus_tag="BK733_RS07275"
                     /old_locus_tag="BK733_06910"
     CDS             complement(10001..11941)
                     /NRPS_PKS="Domain: AMP-binding (99-521). E-value: 3.9e-82.
                     Score: 267.9. Matches aSDomain:
                     nrpspksdomains_BK733_RS07275_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017560287.1"
                     /locus_tag="BK733_RS07275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06910"
                     /product="AMP-binding protein"
                     /protein_id="WP_000806506.1"
                     /sec_met_domain="AMP-binding (E-value: 1.8e-89, bitscore:
                     293.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQAVWFPTEEYKEKTRLYSWMKSLGYEDYETFYNKSIEETAWLW
                     GEAEKAVGYQWMKPYTEVLDLENGTPFAQWYNGGTCNVVESALSRWLADEETRIQPALQ
                     YEGENGTSKSFTYEELDNWVSRVANGLKHAGIEKGDRVTIYMPMIPETVVAMLAVMKIG
                     AIISPIFSGFASDAVMTRVQAAGSKMIITADGFSRRGKIVSLKDEVDKACEHCPTVEKV
                     VIVRHAGNDFTPHNYDFSWSTLEKEKPFIHAEEMQSDDPLMLIYTSGTTGKPKGTVHTH
                     AGFPLKAAFDAGFGMNIKQGDRVLWVTDMGWMMGPFLLFGSLINGATMVMYEGVPDFPE
                     ADRLWETVDKYEITHLGISPTLIRALMAKGDEYVNKHSLKSLEVFASTGEPWNPDPWMW
                     LFETVGKGNVPICNYSGGTEISGGIFGNVLIKPIAPISFNASLPGMAAVVLDDQGKPIR
                     DEVGELCLEKPWVGMTKSFWEDDERYVNTYWSRFENKWVHGDWVIYDGEQYIITGRSDD
                     TLNIAGKRIGPAEYESILVKHNDVIEAAAIGVPDEVKGEVCHCFAVLREGVIFTEDLKK
                     ELMSLVNSHIGKALCPKDIHVVEDLPKTRNSKVMRRVIKAAYLGKELGDLSSLVNPEVV
                     PFIQGLQSSKL"
     CDS_motif       complement(10346..10408)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS07275_0002"
                     /evalue="7.80E-04"
                     /label="NRPS-A_a8"
                     /locus_tag="BK733_RS07275"
                     /protein_end="532"
                     /protein_start="511"
                     /score="12.3"
                     /tool="antismash"
                     /translation="TGRSDDTLNIAGKRIGPAEYE"
     aSDomain        complement(10379..11644)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS07275_AMP-binding.1"
                     /evalue="3.90E-82"
                     /label="BK733_RS07275_AMP-binding.1"
                     /locus_tag="BK733_RS07275"
                     /protein_end="521"
                     /protein_start="99"
                     /score="267.9"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="QPALQYEGENGTSKSFTYEELDNWVSRVANGLKHAGIEKGDRVTI
                     YMPMIPETVVAMLAVMKIGAIISPIFSGFASDAVMTRVQAAGSKMIITADGFSRRGKIV
                     SLKDEVDKACEHCPTVEKVVIVRHAGNDFTPHNYDFSWSTLEKEKPFIHAEEMQSDDPL
                     MLIYTSGTTGKPKGTVHTHAGFPLKAAFDAGFGMNIKQGDRVLWVTDMGWMMGPFLLFG
                     SLINGATMVMYEGVPDFPEADRLWETVDKYEITHLGISPTLIRALMAKGDEYVNKHSLK
                     SLEVFASTGEPWNPDPWMWLFETVGKGNVPICNYSGGTEISGGIFGNVLIKPIAPISFN
                     ASLPGMAAVVLDDQGKPIRDEVGELCLEKPWVGMTKSFWEDDERYVNTYWSRFENKWVH
                     GDWVIYDGEQYIITGRSDDTLNI"
     CDS_motif       complement(11099..11155)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS07275_0001"
                     /evalue="7.60E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="BK733_RS07275"
                     /protein_end="281"
                     /protein_start="262"
                     /score="24.7"
                     /tool="antismash"
                     /translation="MLIYTSGTTGKPKGTVHTH"
     gene            complement(11991..13532)
                     /locus_tag="BK733_RS07280"
                     /old_locus_tag="BK733_06915"
     CDS             complement(11991..13532)
                     /EC_number="6.-.-.-"
                     /GO_function="GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389703.2"
                     /locus_tag="BK733_RS07280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06915"
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="WP_000886755.1"
                     /transl_table=11
                     /translation="MLDQKQQSNTFEERVETIKQGGAPKYHEQNKAKGKLFVRDRLALL
                     FDNGEYVEDALFANCEQTGLPADGVVTATGKIHGRTACVMANDSTVKAGSWGSRTVEKI
                     LRIQETAEKLRVPLFYLVDSAGARITDQVEMFPGRRGAGRIFYNQVKLSGKVPQVCLLF
                     GPSAAGGAYIPAFCDVVMMVEGNASMYLGSPRMAEMVIGEKVTLEEMGGARMHCSVSGC
                     GDVLCKTEEDAITQARQYISYFPNNYLEKTPLVTPQEPKQFDKTLEQIIPENQNAPFNM
                     KDLINRVIDEGSFYEVKKLFAQELITGLARIDGKPVGIIANQPRMKGGVLFHDSADKAA
                     KFINLCDAYHIPLLFLADVPGFMIGTKVERAGIIRHGAKMISAMSEATVPKISIVVRKA
                     YGAGLYAMAGPAFEPDCCLALPTASIAVMGPEAAVNAVYANKIAALPEEERASFIAEKR
                     EEYKKDIDIYHLASEMVIDGIVHPNNLREELKGRFEMYMSKYQVFTDRKHPVYPV"
     gene            complement(13535..14323)
                     /locus_tag="BK733_RS07285"
                     /old_locus_tag="BK733_06920"
     CDS             complement(13535..14323)
                     /EC_number="4.2.1.17"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: ECH (14-254). E-value: 5.8e-74. Score:
                     240.4. Matches aSDomain:
                     nrpspksdomains_BK733_RS07285_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990763.1"
                     /locus_tag="BK733_RS07285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06920"
                     /product="enoyl-CoA hydratase"
                     /protein_id="WP_063547339.1"
                     /transl_table=11
                     /translation="MLQLQNISVDYVTPHVVKISLYRERQANSLSLALLDELQTILTQI
                     SEDSNVRVVILTGAGEKAFCAGADLKERANMNEEQVRHAVSMIRTTMEMVEQLPQPVIA
                     AINGIALGGGTELSLACDFRIAAECTSLGLTETTLAIIPGAGGTQRLPRLIGVGRAKEL
                     IYTGRRISAQEAKEYGLVEFVVPANLLEEKAIEIAEKIASNGPIAVRLAKKAISNGIQV
                     DLHTGLQMEKQAYEGVIHTKDRLEGLQAFKEKRTPTYKGE"
     aSDomain        complement(13562..14281)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS07285_ECH.1"
                     /evalue="5.80E-74"
                     /label="BK733_RS07285_ECH.1"
                     /locus_tag="BK733_RS07285"
                     /protein_end="254"
                     /protein_start="14"
                     /score="240.4"
                     /tool="antismash"
                     /translation="HVVKISLYRERQANSLSLALLDELQTILTQISEDSNVRVVILTGA
                     GEKAFCAGADLKERANMNEEQVRHAVSMIRTTMEMVEQLPQPVIAAINGIALGGGTELS
                     LACDFRIAAECTSLGLTETTLAIIPGAGGTQRLPRLIGVGRAKELIYTGRRISAQEAKE
                     YGLVEFVVPANLLEEKAIEIAEKIASNGPIAVRLAKKAISNGIQVDLHTGLQMEKQAYE
                     GVIHTKDRLEGLQAFKEK"
     gene            complement(14328..15239)
                     /gene="mvaB"
                     /locus_tag="BK733_RS07290"
                     /old_locus_tag="BK733_06925"
     CDS             complement(14328..15239)
                     /EC_number="4.1.3.4"
                     /codon_start=1
                     /gene="mvaB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: HMGL-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1271: 2-isopropylmalate synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017149491.1"
                     /locus_tag="BK733_RS07290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06925"
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="WP_063547338.1"
                     /sec_met_domain="HMGL-like (E-value: 4.7e-50, bitscore:
                     163.6, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLPNFAVIKEVGPRDGLQNEKKIVGTKDKVKWIQLLTEAGLSYV
                     EVSSFVHPKWVPALADANDVFSELKRDPNVTYAALVPNQNGLERAFLQNVDEVNVFLSA
                     SESHNKSNINKSIKEALAVIEDITKQATFEGKKVRGYVSTVFGCPYEGDISVTAVDELC
                     NQLFSYGIYEVSLGDTIGVANPLQVEQVLEHLLKKYDASQFAMHFHNTYGMALANVVKS
                     LEYGIKTFDSSCGGLGGCPYAPGASGNVATDDLVHMLHKLGVQTNIDEEKLLRASQFIQ
                     SKLNIQLPSHVYRALQHKTISR"
     gene            complement(15261..15476)
                     /locus_tag="BK733_RS07295"
                     /old_locus_tag="BK733_06930"
     CDS             complement(15261..15476)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001987345.1"
                     /locus_tag="BK733_RS07295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06930"
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="WP_001987345.1"
                     /transl_table=11
                     /translation="MMTKVYASMAGNVWKIVVGVGDTVEEEQDVVILESMKMEIPIISE
                     EAGTVMKINVQEGDFVNEGDVLLEIE"
     gene            complement(15503..16840)
                     /locus_tag="BK733_RS07300"
                     /old_locus_tag="BK733_06935"
     CDS             complement(15503..16840)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1090: acetyl-CoA carboxylase biotin carboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486723.1"
                     /locus_tag="BK733_RS07300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06935"
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="WP_063547337.1"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 6.6e-12,
                     bitscore: 38.4, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 8.8e-08, bitscore:
                     25.4, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 7.3e-08, bitscore: 25.3,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 1.2e-10, bitscore:
                     34.3, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFQKILIANRGEIAVRIMKTCQKLGIRTVAIYSEADENALHVKMA
                     NEAYLVGGPRVQESYLNLEKIIEIAKKTNAEAIHPGYGLLSENPSFPVRCKEEGIVFIG
                     PSEEIITKMGSKIESRIAMQAADVPVVPGITTNIETAEEAIEIAKQIGYPLMLKASAGG
                     GGIGMQLMETEQTLTKAFESNKTRAQNFFGNGEMYLERYIADAHHIEIQLLADTHGNTV
                     YLWERECSVQRRNQKVIEEAPSPFLDEGTRKAMGEVAVQAAKALGYTNAGTVEFLVDDQ
                     KNFYFLEMNTRLQVEHPVTEEITGLDLVEQQLLIASGEKLSFTQDDVKRSGHAIEARIY
                     AEDPKTFFPSPGKITDLTLPSNVRIDHFLENHVTITPFYDPMIAKVIAHGETREEAILK
                     LHDALEELKVEGIKTNTPMLLQVLEDDVFKSGIYTTGFVTKQLVKK"
     gene            complement(16856..18001)
                     /locus_tag="BK733_RS07305"
                     /old_locus_tag="BK733_06940"
     CDS             complement(16856..18001)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001980414.1"
                     /locus_tag="BK733_RS07305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06940"
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="WP_000397279.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 4.4e-41, bitscore:
                     133.2, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 1.4e-27, bitscore:
                     88.9, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1.8e-58, bitscore:
                     190.1, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFTLTREKQMIKEMVRDFAEKEIAPKAVYYDKTAEFPYETFQKM
                     GELGLLGIPFPEEYGGSGGDTVSYALAVEEIGRACGGTGLSYAATISLGASPIYYFGTE
                     EQKQKYLVPMASGKTLGAFGLTEPNAGSDAGGTQTKAVLDGDEYVISGEKCWITNAEYA
                     NTIIVTAVNGIEENGRKRISAFIVPTTSEGLTISSPYDKMGVRASNTCEIVLDGVRVPK
                     ENILGDVNKGFKQFLYTLDGGRISIAALAVGIAQSAFERALQYAKERQQFGKSISNFQA
                     IQFKLADMATEVELARNLVHKAAWLKDNDKPFGKEAAMAKLFASEAASRIANQAVQIHG
                     GYGYMREYEVERHIRDAKLLEIGEGTSEIQRLVIARHLGCR"
     gene            complement(18218..19186)
                     /locus_tag="BK733_RS07310"
                     /old_locus_tag="BK733_06945"
     CDS             complement(18218..19186)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248633.1"
                     /locus_tag="BK733_RS07310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06945"
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="WP_088020316.1"
                     /transl_table=11
                     /translation="MRQFAKPTIVVSKCLEFDACRYNGEMIPDVTIRNLQPFVTFIPVC
                     PEVEIGLGTPRETIRIVEENGENRLVQPSTREDITEKMEQFSNDFLQTIPDVDGFILKN
                     RSPSCGTRDVKIYSGFEKAPAKGKGSGLFGGAVVKKFSHLPIEEEGRLSNFIIREHFFT
                     RLFTIAYYKMIKHSKNIKDLVSFQSDNKYLFMAYNQVKQKELGRIIANQKNEKIEVVFE
                     NYEKTLYELFMRAPRYTSNVNVCEHIFGYFKTKLKKQEKDHFIELLQKYAEKKIPLSSL
                     LTILKSWAIRFDEKYLLRQTYFEPYPEALVEISDSGKGRDY"
     gene            19420..19719
                     /locus_tag="BK733_RS07315"
                     /old_locus_tag="BK733_06950"
     CDS             19420..19719
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728146.1"
                     /locus_tag="BK733_RS07315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06950"
                     /product="WGxxGxxG family protein"
                     /protein_id="WP_000728157.1"
                     /transl_table=11
                     /translation="MKKKLSSILSVLALSIMFLGTSTQAEYDGNNMNRVNNTDISTRVN
                     DNNLDRVNNDVRTRNVNTTNDLNDNRDKNNNWAWLGLLGLAGLLGLRRRDKETR"
     gene            complement(19841..20494)
                     /locus_tag="BK733_RS07320"
                     /old_locus_tag="BK733_06955"
     CDS             complement(19841..20494)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168191.1"
                     /locus_tag="BK733_RS07320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06955"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_016512809.1"
                     /transl_table=11
                     /translation="MQKDWLEELIAATNTDKRNERQMRILEAAVDMFGEKGYASTSTSE
                     IAKRAGVAEGTIFRYYKTKKDLLLAVVMPTLTKFAAPFFVQTFAKEIFKSEYESYERLL
                     RVVIHNRFKFAQKHFPMIKILIQEVPFQPELKNEIQQLVETELFSHFKKLIVKFQEEGE
                     IIEIPPSSVLRLTLSAVLGLLLTRFLLLPEEKWNDEAEIENTIQFILYGLTPRF"
     gene            complement(20519..21538)
                     /locus_tag="BK733_RS07325"
                     /old_locus_tag="BK733_06960"
     CDS             complement(20519..21538)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1065: ABC-2 type
                     transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002161721.1"
                     /locus_tag="BK733_RS07325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06960"
                     /product="ABC transporter permease"
                     /protein_id="WP_006928521.1"
                     /transl_table=11
                     /translation="MRVTGVIIRIIRQFFRDKRSLAMMFGAPMLLLWLLSLVFTQKDYI
                     PQIAVVDMPAQIVKVMKNQEASIYEYSKENAISELEKQKVDAILRIENGKMKLLLEGSD
                     SSKNRAVLQVLQKSTEKNDVSIMKPEVNYLHGSKDFTMFDGLGPVLIGFFTFFFVFILS
                     GVSFVRERLSGTLERLLSTPVRRWEIVAGYIIGFGIFAFIQSIIIVSFSVYILDLYVAG
                     SIWLTLLITCMLSLTALTLGTFLSAYANNEFQMIQFIPLVIVPQIFFSGLFPMESMNKW
                     LQMLGKVFPLTYGADAMRQVMIRNQGFTEIALDLTVLLLFSLLFAVGNVFALKKHRKI"
     gene            complement(21535..22259)
                     /locus_tag="BK733_RS07330"
                     /old_locus_tag="BK733_06965"
                     /pseudo=""
     CDS             complement(21535..22259)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187059.1"
                     /locus_tag="BK733_RS07330"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06965"
                     /product="ABC transporter ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MQQPSIILRTVSKSFGKKKCYTIFHCKLKKQRFLVLLDLLDRGKQ
                     RSLK"
     gene            complement(22448..22699)
                     /locus_tag="BK733_RS07335"
                     /old_locus_tag="BK733_06970"
     CDS             complement(22448..22699)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548597.1"
                     /locus_tag="BK733_RS07335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06970"
                     /product="hypothetical protein"
                     /protein_id="WP_000548621.1"
                     /transl_table=11
                     /translation="MHKEYEIEEYTAIEEQIHYYCKCLLVSHPDQIIKYLEKRLEKYAE
                     TLQYAHLYPDTVILPLQQLVIEYSLDVARIRKYMNLKT"
     gene            complement(22915..23757)
                     /locus_tag="BK733_RS07340"
                     /old_locus_tag="BK733_06975"
     CDS             complement(22915..23757)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Metallophos_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002141840.1"
                     /locus_tag="BK733_RS07340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06975"
                     /product="metallophosphoesterase"
                     /protein_id="WP_000727189.1"
                     /sec_met_domain="Metallophos_2 (E-value: 2e-09, bitscore:
                     30.8, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKINRIVMIICILVGFTIFLYLQNNLISITEVKITSSKIPSSF
                     KGYKILQISDLHNKKFGDNQDVLIQKIKSIDPDIIAITGDLIDSKSYDAEVSMQLIREI
                     VKKYPVYFVTGNHEQWSGKYNNLEKELKKYDVNVLRNEHVGIRKGEQEINLLGIDDPEF
                     VTGNRDEGNIIIDEIKKAKIEMQPDKYNVLLSHRPEFIKEYTNERLDLVLSGHAHGGQV
                     RLPFIGGLVAPNQGVLPKYTAGLYVEQNTSMVVSRGLGNSIIPQRILNRPEIVVVQLN"
     gene            complement(24018..24944)
                     /gene="bla"
                     /locus_tag="BK733_RS07345"
                     /old_locus_tag="BK733_06980"
     CDS             complement(24018..24944)
                     /EC_number="3.5.2.6"
                     /GO_function="GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="bla"
                     /gene_functions="resistance (resist) ClassA"
                     /gene_kind="resistance"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101021.1"
                     /locus_tag="BK733_RS07345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06980"
                     /product="class A beta-lactamase Bla1"
                     /protein_id="WP_063549314.1"
                     /transl_table=11
                     /translation="MILKNKRMLKIGICVGILGLSITSLEAFTGGALQVEAKEKTGQVK
                     HKNQATHKEFSQLEKKFDARVGVYAIDTGTNQTISYRSNERFAFASTYKALAAGVLLQQ
                     NSIDTLNEVITFTKEDLVDYSPVTEKHVDTGMKLGEIAEAAVRSSDNTAGNILFNKIGG
                     PKGYEKALRKMGDRVTMSDRFETELNEAIPGDIRDTSTAKAIATNLKAFTVGNALPAEK
                     RKILTEWMKGNATGDKLIRAGVPTDWVVGDKSGAGSYGTRNDIAIVWPPNRAPIIIAIL
                     SSKDEKEATYDNQLIAEATKVIVKALR"
ORIGIN
        1 gactgacacc aataccgcgg taattaggat gaaccgctaa tgttccacag cgcatagtct
       61 ttatactttc ataatccttt ataccaccta atattacgcc aactgatttg tcaccgtcta
      121 aagcaagaaa agaatgttct agcaaattac cttctggtcc caaaaaacgg tgtataaaat
      181 caccttttga aacttccatc ttaataatat aatcggaaaa tccatcttta aaagcttcat
      241 atactaaatc aatatttact tcactacatc ttttatattg aatcatataa aattgccctc
      301 caattgcttg tattaaccgc aacatgtacc catataatat attccatcta ttataaattt
      361 cagctcttaa tttgaaatga acggacattt ccctacatat aacccactat taatttgttt
      421 tataacaaac tatctattat ttactacatt aatctcataa acacgcaaaa aagcaattaa
      481 atgctcacat gctctttcgc atgctagcat tttaaagatt ttttgttctt ctttcgtttt
      541 atcatttgct tgatctgaag cagcttttaa acatagaaat ggttttttat ttatttggca
      601 tacataggcg aaagctgcta cttcttgatc aactgccaat gctccatata cggtatggag
      661 taaatatctc atttctgaac tacgaatacg ttgatctccg gataaaaatg taccgaaatg
      721 tattggatca tatgatcgta ttttttttat tcttcttaca agtgattttg ttgtttcaat
      781 aggtgctgtt ctaccgttat ataaattaaa aacatcttcc cctgagccag ctgcagtaac
      841 atcgtgttgt attgcgttta gtgccactac aatatgaccg tttttcacct tgtttgataa
      901 acttccgcat attcctgtca taaacaactc atctggctga aactcactaa ttaacaattg
      961 tacacaacta gcgcagctta ctttaccaac accagtaaca actgatataa tttccagctc
     1021 attaatagta tgaaaatgaa attcccaagc tgctcttttt tcaacacgtt cactcggata
     1081 atgttgatgc aaatacgtca gttctggttc ccatgcagat actattgcaa tacgtttcat
     1141 tacacgcttc acttccccta ttgtcattta aataagttat ctatactttc tcgttcataa
     1201 tctggaatcc attctttgta tttcttatat aaatctcgta ttatttcttt attcttcgca
     1261 attacttcac atcctctatc atcacacata aacatgacaa tatctttcgt tgcatttatg
     1321 agaaatacat ctggataaga aatttctttt tttcgtccaa atcgaggata cagagcagag
     1381 aaatcctcgt gattagcagc ttgtataagc aattcatact taatatcttc cgcaagacat
     1441 tttaaagaaa aatgatatgt acaatataaa tctgcttctt catcttcaaa tagaaacggt
     1501 aacgtttctt gtcttatatg aaatcttaaa tctcttttct taatatattt attgtataca
     1561 tttattttct gtggagtttt tatgttttcc tttttaaatc tataaacatt tgtcactaca
     1621 aacactacat cttcttttga aaatatgtct tcaaataaga atttattata tgtatatagt
     1681 tgagaaaagt attccatatt taaatcgttg gttccttcta caatttgata tttatctttt
     1741 ccgaaatcaa aatgtaaatg atttccccac tgagaatata agtagggtcc taatgtaact
     1801 cctggaaatg tttgattcat atattctttt aattgcattt ccttgtcctt tctactgtaa
     1861 aattcctttt aaatcccagt atgcttcttc tagccacgga tgtataactt tcaatgtatc
     1921 tttcctttcc cacaaatatg aaatatatac aaaagctgct ttttccaaag atttttccaa
     1981 tccttttttt atttctatta aatatggtgg tgtcccgttt tgatcccatt ctattttatc
     2041 tgcaacaaat aatactaaat ccatcttcgt tgcatgttta cgcaacgttg tatggcaaca
     2101 aattgcattt aaaatttctt catcttctac tttaaatatt tcttttgcga ttactctcga
     2161 taatttttga tgaataatca taggaatttt tctttctgtt tccactattt ctaccccaaa
     2221 ttcttcagca actgcaatcc gctcctcgtt cggaaaaatg ccactaatat catgtaaata
     2281 tcctgcaatt gctgcttttt ctttatctac gtgaaacttt tctgcaatct tttttgcctc
     2341 atttgccaca cgaattgaat gtttatatgt aaactcttta ttatatttta gtagaaaagc
     2401 tttaatatca ttgtctattt ttccagttgg agtaaataaa tatatatcat tacataacat
     2461 agaaaatccc ccttataact aacttccatt ataatgtaga atctaaatat ccttgttatt
     2521 ttatattcgt tattaatctt tttactcctt tttaatacgt tacatgaaat tttatacaga
     2581 atcatcgcta caagtacata aatgtttgat attatagtaa tgttgatgat ttgtaaggaa
     2641 aggaactaca caatgaataa aaatatatta attattgatg atgataaaga aattgttgaa
     2701 ttacttgctg tttatttgcg aaatgaaggc tataacattt ataaagctta tgatggcgat
     2761 gaagcattgc aaaaggtttc tacatatgaa gttgatctta tgattttgga tattatgatg
     2821 ccaaaacgaa acggattaga agtttgtcag gaagtgcgtg aaaataatac tgtaccaatt
     2881 ctaatgctta gtgcaaaagc agaagatatg gataaaatat taggccttat gacaggtgcc
     2941 gatgattata tgatcaaacc attcaatcca ttagaattag tagctagagt gaaagcttta
     3001 ttacgaagat cctctttcca aaatgctact tcgcaaaaga atgaagatgg tattattcgt
     3061 attcgctctg ttgaaataca caaacataat catacagtta aagtaaatgg agagtatatt
     3121 aaactaacct ctattgaatt cgatatttta tatttacttg cgagtaatac aggaagagta
     3181 tttagttcag aagaaatttt cgaacgcgtt tggaatgaag atggttatgg ttcaaataag
     3241 acggtaatgg tccatattag caacttacga gataaacttg aaacagtaat gaatggagaa
     3301 aaattgattc atactgtttg gggagttggc tataaaattg agaaatgaag catttgatat
     3361 attaaaagat attcctaaat ggaaacttat tttttggctt ttattagcta tcacactcac
     3421 tcctattact gaactaatta tttatcgcct cgttacgagt gtaattgaga gttcacttac
     3481 tctaactgaa ctcggtaaag aattcattcg aatttttggt tatgaaaagt ataaagggat
     3541 taaagagtct ttatggatga tgattgtatc ttatttgttt ttattttcta ttttttcatc
     3601 ctatctttac attttttatc gtcacgagaa aaaactttat tatgaaactt gtataaaaaa
     3661 aatgatagaa gaaattcgtt atattgcaaa tggaaacttc aatcataaag tttctattgt
     3721 aaatcataat tatctagaag aattagcaat cggggtcaat gaaattgttg aacaattaaa
     3781 agtctccatt gatgaagaaa gacaagcgga acaagcaaaa agtgaactta ttacaaatgt
     3841 atcacatgac ttacgcaccc cccttacttc tatagttgga tacgtaaatt taatccatca
     3901 tgataattat agagatgaag tggaattgcg atactatatt caagtcattt acgataaagt
     3961 aactcgtctt aatgcattaa tgaatgactt gttcgaatat acacgtgttc aaaataaaga
     4021 attaagcttg tattctgttc ctattgatat tgtagagtta cttggacaat taactgttca
     4081 attccgcata caggttcaag aagctaatat agaatgtcgg ccttctttcc cgtctcaaaa
     4141 gcttatggta ttagctgatg gtgacaaatt agtgcgtgtg tttgaaaatc ttattataaa
     4201 cgctatcgct tatggaagcg aaggtcgcta tattgacctt actgcttatg aaagcaataa
     4261 tatgattaca attgatataa caaattacgg acaacctatt cctagtacgg atttacctca
     4321 tatttttgaa cgtttttacc gcgttgagaa atctcgctct acaaacactg gtggatctgg
     4381 acttggatta gcaattgcaa aaagtatagt cgagttgcat aaaggaacaa ttgaagtata
     4441 tagtgatgac gaaaaaacaa cattcactgt aaaactactt ccatacaaat gttaatatta
     4501 caaataaaaa gttcaatttc ttatcatata agaaattgaa ctttttctat tatttcccta
     4561 aatttaataa tcctttataa ttctttagaa attctttacc ttttctttct tcctaattaa
     4621 gcattccttt ttacaatact attattgatg tcataatagg aggatcactt atgcaattct
     4681 taaaaaaatt cgctatttta ttattatctt ttttcattac tgcacttatc gttttatatt
     4741 tttatcttta tgtaaatggt ccgattattc aagctaaatc agctatttta ataaatgcta
     4801 attcaggtga aattgtttat aaaaaaaatg aagaaacccc agtacaatca gctactttat
     4861 ctaaattaat gacggaatat atcgtattgg aacaactaaa tgatagaaaa atacagttag
     4921 atgacttcgt acaaataagt aacgaagttt tccgagtaga aacaagccct atacaagtaa
     4981 catcgaatga taaaacaacg gttcgtgatt tacttcatgc attgctactg gcagggaata
     5041 atcgttctgc actcgctcta gcagaacaca ttgctggaac tgaagataac tttacaatat
     5101 taatgaataa aaaagcaaaa gaactacagt tattacaacc atctccattt ctaaactcta
     5161 ctggaattaa tcatgataca aataaacaat caactacaac agcaatagac gcagctaaac
     5221 tagctgcacg cttagtaaaa gattttccag atgtattgaa tattacgaaa ctaacctctt
     5281 accaatttac cttcaaagat tcccaggttt tcaatacgaa taaaatgata tattccctta
     5341 atgaaaacat taaacttcaa ggtgttgatg gcttacaaac tagtttttca actaacggta
     5401 attatagttt tgtcagtaca gcaaaactag gagatactag actaatttca gtaatattag
     5461 atgcagatga agaaaacatc tcatttattg aaacaaaaaa gttgctacag tatggttttg
     5521 atccttcctc gtactctgca ctccaagctt ttaaagacac ccttacatct tggacaatat
     5581 tgcttcagtt taaaaactta attatacaaa ctataatgat tttcttaatc attaccacct
     5641 taatgttttt acatatacgt cagaaaaaat ccgaagattt caattaagaa atcttcttat
     5701 atataaaaac cgacttgcgc ttgagatgca agtcggtttt taattctatc atcctcgtcc
     5761 aatcaaattt gctgaaactg gttgcaagtt taactccctc gcccatatag atagctgacc
     5821 aatatgatgg atttcatgta cgattacatg tcttaaaacc tatgtttttt gaggcataca
     5881 tccatctctg tttcctttcg ttgtattcgt ttgtttagtt taaatgggtt aatctattaa
     5941 ttctattaaa tccaacaatt taactattac actttaaagt tctattcata tactatagta
     6001 gattactaga aaggaggttt aactatggat agttgcgttg tatttgtaaa cggacaacct
     6061 tttttagttc tttcagtagc tggtattgaa attgctagat tagagatttc ccttcaagta
     6121 gcattagctc taagagtgct tgggatacca atctgtgatt aattaccaaa ttaataagaa
     6181 aagagggcat tagccctctt tttttatttt cttctcacct tttcttaaca acaaataaaa
     6241 tttaaatttt atttgttata ttgtaatgaa aatccgaaaa atcaacccat tttattcaat
     6301 aaaaaagagc accacccata cctcattatc cgtttctata atattcaagt ttgattaata
     6361 ataaaattaa agtatatgaa cgttacatga aacacttgtt aagatagctc tccttcacac
     6421 tatttatttc gctgacgcta tattatcaaa gcacacaatg attaactttt tatacggctt
     6481 attttaagta tgcttgccaa tacttttact tgtatgttct atacactcat tctctacggt
     6541 atactgatac caacttatat tttttgatga gggcccattt ccctatatcc ctctttattc
     6601 ccatgtactt tttttctcaa atccaagcta aataatctat attgttatct tttaaaatca
     6661 gctcactatc tgattatatt aataacctat cacctggtaa aatcctatta cctaatttat
     6721 cttcaattga atggtccatc atttatactt acggattggc tttcccgtat ctattgtgca
     6781 tggatattgg attttctagc atctagctca tttctttaaa aatgtcggat ttttcgtcta
     6841 ttattaaatg aataatgttt ggaaggggat ataatatgac agaaattaaa gtagggctta
     6901 cacaattttt agacttcaca ctgaaaggta gtacagctaa aacaaccttt ataaaaaatc
     6961 taaaatccca acctgaatat caaccagctt ttgattactg gaaacaatta cgagaaactg
     7021 ttattaaatt tcatcaaaac agactacctt ttgaatattt cgaaacatta gtacaaacgg
     7081 ttgatcagaa aaaaaaacaa aactacattg atgtcataaa gcaatacaaa aaattcatta
     7141 aaaataaaga catctcttgg tttgatccag gtaaatcaca ttggaaatca gatgatttaa
     7201 tcgtccgttc atcacctgaa cttggcttat tcattaataa tgagccacat cttatcaaac
     7261 tgtttttcaa aggaaaaaaa gagcgaattg ataagtacaa cattaactcg acacttacac
     7321 tattaaatga atcaacattt tcaaatgaac gaaacgatgt gaattatacg gtacttaaca
     7381 tccaaaagaa cagaatgtat acgaataact ctattaataa taatcattta gtagcgctta
     7441 aatcagaagc taaccagttt tgctatatat ggaataatct ataaaggtta ctattcatcc
     7501 cttttaatac cttgttgatg catactttag ttacaatttt tacagattca ttcagtttcg
     7561 tttaattagg gctcaccctc ttattcctca gaaacaatgc aggtgggcct ttttcaaaaa
     7621 tggtttcttt gtcagtttta acatactagc tctcccccga aacagctgca attgagatta
     7681 tttttgtttt attatccact atacccacca agcccttttg ttaacataat ataattataa
     7741 attactattg ttgctacttc ttccattcat attctaacat actcatttcc cataaattcc
     7801 aatacgtttc tcctactctt tttgactctc ttaataagcc ttcttttaca aaccctattt
     7861 tttcgtaaca tgaaatggcc gatatattaa aatcatacac accaagagtt actctatgta
     7921 gttttaattc gtcaaaagca atatgaagta ctgctttcat catatgtttt cctatagaac
     7981 gtcctctcat cttcgtatta ccaactaata cttttccaat tctagcggac ttattaatat
     8041 tatctatttg cccaagcgaa atatgaccaa taacgtctga agtctcttca tctaccactt
     8101 tgaaagcgag tgtatttgca ctttctatat acttctctaa ttgctgttcg tctaaaggga
     8161 atgtgaatgc atttcctgac cattgtatta gaaattcttc agaattaatc caatttatta
     8221 attgtttaaa atcagatttt ttaaaggttt ttagtttaat cacttccatg ctcctttcgt
     8281 acatcatact ttataacctc aaacttattt ctatataaag aatatattct ccttttatat
     8341 gaaattaaaa taaagccatt catgtgaatg gctttacaaa aaagcagcat aattatgctt
     8401 tgtttgctac atcaatacca ttaaaatcaa agtcagcagt aaccgttgta acaggtggga
     8461 aaattgcttc aattttacaa atggaaataa ttactccaac cgatccagga atagccgcaa
     8521 gcctaactac atcacaactt acagacgaaa cagtacctac ataagtacgt ccctctgtat
     8581 taatttgaac tgttcgatta attaatttct taagttcatc tctaattcct tgtacacaag
     8641 aattatcaca tttctcatgc tgttcttttt tacactcttc gaatacttcc caacaatcat
     8701 cttgtttaca tgtacacttc tttttacaat tattaaatcg agaacccaat acctcacccc
     8761 ctaatccttt ctattagtaa agtatgtcca ctagcttaaa agggtacgag gcaaacacat
     8821 aaaaagaaaa aaacaaacaa ttgtaccaaa ccttcttttc attattcatt ataaaaacag
     8881 acttctccta attaaaatct tgtttaatga gcggccgtga atttagtcct actccattta
     8941 atgtttagac accactttct aaaacaaatc tttttctttt aataagatgc cccttaaaaa
     9001 cacactagat tttcccatta actcacatat acatagactt tatttgtgga tttcccattt
     9061 ttcattgtga aatatacatt taaaaaagcg tactcgaaaa taagatatta tatgtaatca
     9121 gaacgctttc ttcaacaaat gtttattgga aaacaccata actagtagta gttctctcca
     9181 ctatctctta aaaattagca tagtttgtat gaactctata tcgagtagaa aacactctat
     9241 catataaaga taaaatagta ggttaataaa caagccattt tttttattaa aacatagaat
     9301 aatatgaaca ctattgaaag gaggtagaaa tgaatgtctt ggaaaaatga ttgctggagt
     9361 tctcagaaaa agcattgttg tgatgattgg tggaaatcta aagaaaagca tcattgtgat
     9421 gatgattgga gttctcagaa aaagcattgt aatgatgatt ggtggaaatc tgaagaaaag
     9481 tgtcatcgtg atgatgattg gagttttaaa cgaaataagt gtcgttgtaa atgtttcttt
     9541 atttgttgcc attgtaaaag aaaaaattgg taatattttt aatgtcaacg taaagaaaaa
     9601 aatatgtctt ttggaatcaa tcgggctttt ccggaaagtt gatccctcac ctacttcctt
     9661 gctttcgctg aatttttagg tgggggcatt actgcccggc aaatagcgag ataaatacta
     9721 ggatgtaata tataaaaagc tgactgagta tagtcagctt tttatcaatg aatacgtaaa
     9781 ggtttaaacc ggcatataag tagtcgcgct ttgtcatcac cttgttgttg taaattagta
     9841 tttctccatt gaatagacat tccataatat cgtattttga ttaaaactaa aaaacatact
     9901 taacaccaat agtagaattt ttcttcttca tcaaattcac tttttttctt tttattaaca
     9961 taaaaaagga gctctgttta tatagagctc ctttctaatt ttataattta ctagactgta
    10021 acccctgaat aaacggtact acttcaggat ttacaagtga cgacaaatca cctaattctt
    10081 ttcctaaata agctgcttta ataacacggc gcattacttt agaattacgt gttttcggta
    10141 aatcctctac aacgtggatg tctttaggac acaacgcttt gccaatatgt gagtttacta
    10201 aactcattaa ttctttcttt aaatcttctg taaatattac cccttctcgt aatacagcaa
    10261 agcaatgaca aacttcacct ttcacttcgt caggtacacc aattgcagca gcttcgatta
    10321 catcgttatg tttcacaaga atagattcat attcagcagg tccaatacgt ttacctgcaa
    10381 tatttaacgt atcgtctgaa cgccctgtga tgatatattg ttcaccatcg taaatgaccc
    10441 agtcaccgtg gacccattta ttttcaaaac gtgaccaata tgtgttcacg taacgttcat
    10501 catcttccca gaagcttttc gtcataccta cccaaggttt ctctaagcat aattctccga
    10561 cttcatctcg aattggttta ccttgatcat ccagtacgac agctgccatt cctggtaaag
    10621 atgcgttaaa actaattggt gcgattggtt taataagaac attaccgaaa atcccaccag
    10681 agatttcagt tccacctgag tagttacaga ttggaacatt tcctttacca actgtttcaa
    10741 acagccacat ccatggatca ggattccaag gttctcctgt tgatgcgaat acttccaaac
    10801 tctttaaaga atgtttattg acatactcat caccttttgc cattaatgct cggattaacg
    10861 ttggcgaaat acctagatga gtaatttcat atttatcaac tgtctcccat aaccggtctg
    10921 cttctgggaa gtctggaaca ccttcgtaca taaccatcgt tgctccatta atgagtgaac
    10981 cgaataataa aaatggcccc atcatccaac ccatatcagt tacccataat acacgatcac
    11041 cttgtttaat attcattcca aatcctgcat caaaagctgc tttcaaagga aatcctgcat
    11101 gcgtgtgtac tgttcctttc ggcttaccag tcgttcctga tgtataaatg agcattaatg
    11161 gatcatcact ttgcatttcc tcggcatgta tgaatggttt ttctttttct agcgtactcc
    11221 acgaaaagtc atagttatgt ggtgtaaaat catttcctgc atgacgaacg ataacaactt
    11281 tttcaacagt tgggcaatgt tcacaagctt tatctacttc atcttttaaa gaaacaattt
    11341 taccgcggcg tgaaaatcca tctgcagtaa taatcatttt tgaacccgcc gcttgcacac
    11401 gtgtcataac tgcatcagac gcaaagcctg agaatattgg tgaaataatt gctccaattt
    11461 tcattacagc tagcatcgca acaactgttt ctggaatcat cggcatataa attgttacac
    11521 ggtcaccttt ttcaataccc gcgtgtttca aaccattggc aacacggctt acccagttat
    11581 caagctcttc atatgtaaat gattttgaag ttccattttc tccttcgtac tgaagtgctg
    11641 gctgtattct tgtctcttca tccgcaagcc agcgggataa agctgattct acaacgttac
    11701 atgttccgcc attataccac tgtgcaaacg gtgtaccatt ttctaaatct aacacctctg
    11761 tataaggttt catccattga tagccaactg ctttttctgc ctctccccaa agccaagctg
    11821 tttcttcaat agatttatta taaaaagttt catagtcttc atatcccaat gatttcatcc
    11881 aactatataa acgtgttttt tccttgtact cttctgttgg aaaccaaact gcttgtttca
    11941 atgttttccc ctccttaact ttttgagcaa gaaagcccta aatagggctt ttaaactgga
    12001 tatacaggat gtttacgatc cgtgaatact tgatatttac tcatatacat ttcgaaacgt
    12061 ccttttaact cttcacgtaa attgtttgga tgaacaatac catcaatcac catctctgat
    12121 gctaagtggt atatatcaat atctttctta tactcttcac gtttttcagc aataaagcta
    12181 gcacgctctt cttctggtaa agctgcaatt ttatttgcat atacagcatt gaccgcagct
    12241 tctggaccca ttaccgcaat agaggctgtc ggtaatgcca ggcagcaatc tggttcaaat
    12301 gctggacctg ccatcgcata taaaccagca ccatatgctt tacgaacaac gatagaaatc
    12361 ttcggtacag ttgcttcact cattgcagaa atcatttttg caccgtggcg aataatacca
    12421 gcacgctcta cttttgtacc gatcataaat cctggtacat ccgcaaggaa taataatgga
    12481 atatgatatg cgtcgcataa attaataaac ttagcagctt tatcagctga atcatggaat
    12541 aatacgccgc ccttcatacg cggctgattt gcaataatac caactggctt accatcaata
    12601 cgtgctaagc ctgtaattag ctcttgagca aataattttt ttacttcata gaatgaacct
    12661 tcatcaataa ctctattaat gagatctttc atattgaaag gagcattttg attttctgga
    12721 atgatttgtt ctaacgtttt atcgaattgt ttcggttctt gaggtgtaac caatggagtc
    12781 ttttctaagt agttatttgg aaaatatgaa atgtattgtc ttgcttgtgt aatcgcatct
    12841 tcttctgtct tacataaaac atctccgcat cctgatacag agcaatgcat acgagctccg
    12901 cccatttctt ctaaagttac cttctcaccg ataaccatct cagccatacg aggagatcct
    12961 aaatacatag atgcatttcc ttctaccatc attacaacgt cgcaaaaggc tggaatatag
    13021 gcaccaccag ctgcagaagg tccaaataat aagcatactt gaggaacttt acctgataat
    13081 ttcacttgat tatagaagat tctccctgca ccacggcgcc ctgggaacat ttctacttga
    13141 tctgtaatac gtgctccagc agagtcgact aaataaaata acggaacacg tagtttttca
    13201 gctgtttctt gaatacgtaa aatcttttca actgtgcgtg atccccacga tccagctttt
    13261 acagtagaat cattggccat tacacacgca gtacgaccat gtattttacc cgttgccgtt
    13321 acaacgccgt ccgcaggtaa gcctgtttgt tcacagttag caaataatgc atcttctaca
    13381 tattcgccat tatcaaatag aagcgctaaa cgatctcgaa caaataattt tccttttgct
    13441 ttattttgtt catgatattt tggtgcgccg ccttgtttaa tcgtttcaac tcgttcttca
    13501 aatgtatttg attgttgttt ttgatctaac atatttactc ccccttatac gttggtgtgc
    13561 gtttttcctt gaatgcttgt aatccttcta gtctatcttt cgtatggatt acaccttcat
    13621 acgcttgttt ttccatttgt aatccggtat gtaaatcaac ttgaataccg tttgaaatcg
    13681 ctttttttgc taatcgaaca gcaataggtc cattactagc aattttctct gctatttcaa
    13741 tcgctttctc ttcaagtaaa ttagctggta caacaaattc tactaaacca tattcttttg
    13801 cttcttgcgc tgaaatacgt ctacctgtat aaattaattc tttcgctcta ccaactccaa
    13861 ttaatctcgg taagcgctgt gtaccacctg ctcctggtat aattgcaagt gtcgtttcag
    13921 taagaccaag acttgtacat tcagcagcaa ttctaaaatc acaagctaaa cttaattccg
    13981 taccaccacc aagtgcgatt ccatttatag cagcaataac tggctgtggt aattgttcaa
    14041 ccatctccat agtagtgcga atcatactaa cagcatggcg aacttgttct tcattcatat
    14101 tggcacgctc ttttaaatct gcaccagcac aaaatgcttt ttcaccagca ccagtcaaaa
    14161 taactacacg cacgtttgaa tcttcactta tttgagttaa tatagtttgt aattcatcta
    14221 ataatgctaa agataatgag tttgcttgtc tttcacgata taacgaaatc tttacaacgt
    14281 gaggtgttac ataatcaact gaaatgtttt gtaattgtag catcttctca cctacttatc
    14341 gttttatgtt gaagcgctct atacacatga ctcggtaatt ggatatttaa tttactttga
    14401 ataaattgac ttgctctcaa taacttctct tcatcaatat tcgtttggac tcctaactta
    14461 tggagcatat gtactaggtc atcagtagca acattacctg atgctcctgg tgcataaggg
    14521 cagcccccga gtccaccaca agaactatca aacgttttaa taccgtattc taatgattta
    14581 actacattcg ctagagccat tccatacgta ttatgaaaat gcatggcaaa ctgcgaagca
    14641 tcatacttct ttaatagatg ttctaatact tgttctactt gtaatggatt cgctacaccg
    14701 attgtatccc caagagaaac ttcataaatg ccgtatgaaa atagttgatt acataattcg
    14761 tcaactgctg tgacacttat atccccttca taaggacatc caaatacagt tgaaacatag
    14821 cctcttactt ttttaccttc aaatgttgcc tgctttgtta tatcttctat tacagctaat
    14881 gcttctttaa ttgatttatt aatattactt ttattatgag attcacttgc tgataaaaaa
    14941 acattcacct catctacatt ttgcaaaaaa gctcgttcca aaccattttg atttggaaca
    15001 agcgcagcat atgtaacatt aggatccctt ttcagctcag aaaacacatc atttgcatcc
    15061 gctaatgctg ggacccattt tgggtgaacg aatgaggaaa cttcaacata cgacaatcct
    15121 gcttctgtaa gtagttgaat ccattttact ttatcttttg tgccaacaat ttttttttcg
    15181 ttctgtaagc catcacgtgg tcctacttct ttaatgacag caaaattagg tagtttcaat
    15241 tcgcttcccc ctatatctcc ctattcaatt tctagtaata catctccttc atttacaaaa
    15301 tcgccttctt gcacattaat tttcataacc gtgccagctt cttctgaaat gattggaatt
    15361 tccattttca tagattctaa aatgacgaca tcctgctctt cctctactgt atctcctact
    15421 cctacaacaa tcttccaaac gtttcctgcc atcgatgcgt atactttcgt catcattttt
    15481 tcccccttaa tattgttaaa tcttatttct taacgagttg tttcgttaca aaacccgttg
    15541 tataaatacc acttttaaac acatcatctt ctaaaacttg cagtagcatt ggtgtgttcg
    15601 ttttaatacc ttctactttt aattcttcta gagcatcatg taatttcaaa attgcttcct
    15661 cacgagtctc gccatgtgca ataacttttg caatcattgg atcatagaaa ggtgtaatcg
    15721 ttacatgatt ttctaagaag tgatcaatcc gtacatttga cggtaatgtt aaatctgtaa
    15781 ttttcccagg tgatgggaag aatgttttcg gatcctctgc ataaatacga gcttcaatgg
    15841 catgaccact acgttttaca tcatcctgag taaatgatag tttctctcca cttgcgataa
    15901 ggagttgctg ttctacaagg tctaatcccg taatttcttc tgtcactgga tgctctactt
    15961 gtaatctcgt attcatctct aagaaataga agttcttctg atcatctaca agaaactcaa
    16021 ctgtacctgc atttgtatag ccaagtgctt tggcagcttg tacagcaact tctcccatcg
    16081 cctttcgtgt accttcatct aaaaatggtg aaggtgcttc ttcgattact ttttgatttc
    16141 ggcgctgtac cgaacattca cgctcccata aatacactgt gttaccatgt gtatctgcta
    16201 aaagctgaat ttcaatatga tgcgcatctg ctatatatcg ctctaaatac atttctccgt
    16261 taccaaagaa gttttgcgct cttgttttat tactttcaaa cgctttggtg agcgtttgct
    16321 cagtttccat caactgcata ccaatgcctc cgccgcctgc ggatgcctta agcattaagg
    16381 gataaccaat ttgttttgca atttcaattg cttcttcagc agtttcaata tttgtagtaa
    16441 tacctgggac tactgggaca tctgctgctt gcattgcgat acgtgattcg attttacttc
    16501 ccatcttcgt aatgatttct tctgaaggac cgataaatac gattccttct tctttgcaac
    16561 gaaccggaaa agacggattc tctgataata aaccataacc cggatgaatc gcttctgcat
    16621 ttgtcttctt agctatttca ataatttttt caaggtttaa ataactttct tgaacacgcg
    16681 gtccacctac taagtaagct tcatttgcca tcttcacatg aagggcattt tcatctgctt
    16741 cagaataaat agcaacagtg cgtatgccaa gtttttgaca agttttcata atacgaacag
    16801 caatttcccc gcgattcgca attaatattt tttgaaacat agtgattcct cccttttatc
    16861 tacatcctaa atgtctagca attacgagac gttgaatttc agaagttcct tcaccaattt
    16921 ctaatagttt cgcatcacga atatgtcgtt caacttcata ttcacgcata taaccatatc
    16981 caccatgaat ctgtactgct tgattcgcaa tacggcttgc tgcttccgat gcaaatagtt
    17041 ttgccattgc cgcttctttg ccgaaaggtt tatcgttatc ttttaaccaa gctgctttat
    17101 gtactaaatt gcgtgctagc tctacttcag tcgccatatc agctaattta aattgaatcg
    17161 cttggaaatt agagattgac tttccaaatt gttgacgttc tttcgcatat tgtaatgcac
    17221 gttcaaaggc tgattgcgca ataccaactg ccaatgcagc gattgaaata cgccctccat
    17281 caagtgtata taaaaattgt ttaaagcctt tatttacatc accaagaata ttttctttcg
    17341 gtacacgaac accatcaagt acgatttcac aagtattaga agcgcgaaca cccatcttat
    17401 cgtaaggact tgaaatcgtt aatccttcac tcgtagtagg cacgataaat gcagaaatac
    17461 gttttctacc attctcttca ataccgttga cagcggttac aataattgta tttgcatact
    17521 ctgcgtttgt aatccaacac ttctcaccac tgattacata ctcatcacca tctaataccg
    17581 ctttcgtttg agtaccacct gcatctgatc cagcattcgg ctccgttaat ccgaaggcac
    17641 ctaatgtttt acctgacgcc attggaacta aatacttttg tttttgttct tctgtaccga
    17701 aataataaat tggagaagca cctaatgaaa ttgttgcagc atagcttaaa ccggtgccac
    17761 cacaagcgcg accaatttct tcaactgcta acgcatacga taccgtatcg ccacctgaac
    17821 ctccatactc ttctgggaat gggatgccta ataatcctag ttcgcccatt ttttgaaatg
    17881 tttcatatgg aaactctgca gttttgtcat aatatacagc tttcggtgcg atttcctttt
    17941 cagcaaagtc acgtaccatt tctttaatca tttgtttttc gcgagttaga gtaaattcca
    18001 ttccgctaca caccccttat cgaatattga gtttcgacag cgtgcgagtt tctactacaa
    18061 acataacaac ttaaaagaca agaatattag ttagacaatt gctacaattc tgtcataact
    18121 taattttaaa acagattctg tactaatgca caaaaaaatt cctacattgt tctacaacaa
    18181 cataggaggt tttgtgtcga attatgtaat ttattgttta ataatctcta ccttttccgg
    18241 aatcagaaat ttctactaat gcttcagggt atggttcgaa atatgtttgt cttaataaat
    18301 acttttcatc aaatcgaata gcccatgatt ttaaaattgt aagtaaacta ctaagtggta
    18361 ttttcttttc tgcatacttt tgtaacagtt ctataaaatg atctttttct tgttttttta
    18421 actttgtttt aaaatatcca aaaatgtgct cgcatacatt tacattcgat gtataacggg
    18481 gcgcacgcat aaataattca tataaagtct tctcatagtt ttcaaatacc acttcaatct
    18541 tttcattttt ttgatttgca attatacgcc ctaattcctt ttgtttcacc tgattatatg
    18601 ccataaataa atatttattg tccgactgaa acgatacaag gtcttttata tttttactat
    18661 gtttaatcat tttgtaatat gcgattgtaa atagccttgt aaagaaatgc tctctaataa
    18721 taaaattcga caatctgcct tcctcttcaa taggaagatg cgaaaatttt tttactactg
    18781 ctccgccaaa taagcccgat ccttttccct ttgctggcgc tttttcaaaa ccagaataaa
    18841 ttttcacatc acgcgtacca caacttggtg aacgattctt taaaataaaa ccatccacat
    18901 ccggtatcgt ttgtaaaaag tcatttgaaa actgctccat tttttcagtt atatcttctc
    18961 gtgtcgacgg ttgcacaagt ctattctcac cattttcttc tacaattcgt atcgtttcac
    19021 gaggagtccc caatccaatc tcgacttctg gacaaacagg tataaatgta acaaatggct
    19081 gcaaatttcg tattgttaca tctggaatca tctctccatt ataacgacaa gcatcaaatt
    19141 ctaaacattt acttacgacg attgttggtt ttgcaaattg tcgcatctta ttacctcctt
    19201 agtgctagcc cgtataatat aaattgtact aagattaaca ttttaagcaa agtttgattc
    19261 atttattttt atgagaaata tatatccaat gatatattaa caatcattca actctatttt
    19321 ctataaatgt gcgccttatt aatgagggtt tagatattgt gaaatgaata atacacactt
    19381 gcttcggaga aattaatgat taagaaagga gggattgtta tgaaaaagaa attatcatct
    19441 attttaagtg ttttagcact atctattatg tttttaggta catctacgca agctgaatac
    19501 gatggaaata atatgaatag agttaataac actgatattt caactcgagt aaatgacaat
    19561 aacttagaca gagttaataa tgatgtaaga actcgaaatg taaatacaac aaatgattta
    19621 aatgataatc gtgataaaaa taataattgg gcttggcttg gtttattagg actagcagga
    19681 ttattaggtc ttagaagaag agacaaagaa acacgctgat ttagaatgtt tttagtaaac
    19741 aagtacttat ttatcaaaaa tatataatga aaataaatcg cctttttaaa ggcgatttat
    19801 ttttacttaa aagtaatgta tgtcattcac acttcaacga ttaaaatcgt ggagttaatc
    19861 catacaatat aaattgaatc gtattttcaa tttccgcttc atcattccat ttttcttcag
    19921 gcaataataa aaatcgtgtt aataataaac ctaatacagc tgataaagta agacgtagta
    19981 cggaagatgg tggtatctca ataatttccc cttcttcttg aaatttcaca atcaactttt
    20041 tgaaatgtga aaacagttct gtttctacta attgctgtat ttcatttttc agttctggtt
    20101 ggaatggcac ctcttgaatt aatattttta tcataggaaa gtgtttttga gcaaatttaa
    20161 acctattatg aattacaacc cttaaaagtc tttcatatga ttcatactcc gatttaaata
    20221 tttcttttgc aaaggtttgt acgaaaaatg gtgcagcgaa cttagttaaa gttggcatta
    20281 cgaccgctag taataaatct tttttcgtct tatagtagcg gaagattgtt ccttccgcta
    20341 caccagcccg ttttgcaatt tcacttgttg aagttgaagc gtatcctttt tctccaaaca
    20401 tatcaacagc cgcctctaat attcgcattt gacgttcatt tcttttatcc gtattagttg
    20461 cagctattaa ttcctccagc caatccttct gcatttataa agctcctttt tgtcattatt
    20521 atattttgcg atgtttcttt aaagcaaata catttcctac tgcaaataat agtgaaaaaa
    20581 gtaataatac agtaagatct aatgcaatct ctgtaaaccc ttgattccga atcatcactt
    20641 gtctcattgc gtcagcgccg tatgtgagtg gaaatacttt ccctaacatt tgtagccact
    20701 tattcataga ttccattgga aacaatccag aaaagaagat ttgtggcaca ataacaagcg
    20761 gtataaattg aatcatttga aattcattat ttgcgtatgc cgataaaaac gtacctaatg
    20821 ttagtgcagt taaagagagc atacatgtaa taagtaacgt tagccatatg gagccagcta
    20881 cgtataaatc taaaatataa acggaaaaac ttacgataat aatggattgt ataaaggcaa
    20941 agattccaaa accaataata taacctgcga ctatttccca tcttcttact ggggtggata
    21001 ataacctttc tagcgtacca cttaaacgtt ctcttacgaa agatacacct gataatataa
    21061 atacaaagaa aaatgtaaag aaaccaatta atacaggacc aagcccatca aacatcgtaa
    21121 agtctttaga gccatgtaaa taatttactt caggtttcat aatcgataca tcattcttct
    21181 ctgtgctttt ttgcaatact tgaagtacag cacgattttt tgacgaatca ctaccttcca
    21241 acagaagttt catttttcca ttctctattc gaagtattgc atctaccttt tgtttttcta
    21301 actcagaaat cgcgttttct ttactgtatt cataaattga cgcttcttga tttttcatca
    21361 cttttactat ttgagcgggc atatcaacaa cagcaatttg cggtatatag tctttttgtg
    21421 taaacactag cgataatagc caaagtaata acattggtgc cccaaacatc atcgctaacg
    21481 aacgcttatc tcgaaaaaat tggcgaataa tacgaatgat tacaccagta actctcatga
    21541 tgacaccacc tcttccaaca aaaagacatc ttcaatccgt ccagatgaca cgcgtttttt
    21601 caattcttca gttgttccaa ccgcaactag tttcccttct ctaattaacc ctagacgctc
    21661 acaaaattca gcttcatcca taatgtgcgt cgttacaata atcgttgtgc ctttcttttt
    21721 aagatcataa aatttttccc aaatcgtttt tctaagaagc ggatctatcc ccactgtagg
    21781 ctcatctaaa attaatattt ctggttcatg gaggagtgct attgctaatg ataagcgttt
    21841 tttcattcca cctgaaaatt gttgtacttg ctttttcata tactgtgaca attgtacaag
    21901 ttcaaaaacc tctgcaattc ttgtcttttt actttttccc tttaatccat acatcgttgc
    21961 tataaaatct gcattttcat atgctgacaa ttcttcatat aatgcatccg cctgggccat
    22021 ataaccaatt cgtttcattt catttaaatt aggcatatta gtattattaa caagcacttc
    22081 acctttcgtt gcctcattaa tacctgcaat cattttaatg agcgttgttt tccccgatcc
    22141 agaaggtcca acaaaaccaa aaatctctgc tttttcaact tgcaatgaaa gattgtgtaa
    22201 cacttctttt ttccaaaact ttttgataca gtccgtaaaa taattgaagg ttgttgcacg
    22261 tttcatcctc cttttccaat aaaaatgagt gagtactcac ttttatttta ctcttgctta
    22321 attatatgtc aaacttcgtt gattagtatg tatgttcaat caccgatatt aaacaatgaa
    22381 acagagtcga cattagcatt tcatttccta aagtcgactc tgtttcattt aaactataaa
    22441 tttcacttta cgtttttaaa ttcatatact tccgaattct agcaacgtct aaagaatatt
    22501 cgataactaa ttgctgtaac ggtaaaataa ctgtgtcagg atataaatgt gcatattgta
    22561 atgtttctgc atatttttct aacctttttt ctaaatactt tattatttga tcaggatgac
    22621 ttactaataa acatttacaa taataatgga tttgttcctc aatcgccgtg tattcttcaa
    22681 tttcatattc tttatgcatg tgtacacccc tttaagtttt ttatatgaat tacttatcac
    22741 aattttcact cgacaaattt taagtttttt gattatttac actataacaa acgctgaggg
    22801 tcaaattttg ttactttatg taacgtagaa aatttttaac aaaattgtaa tgtttactcc
    22861 tgcaaaatac agaaagcccc tttactcatt tttgtataaa aaaaatcgta cagcttaatt
    22921 tagctgcacg actacaattt ctggcctatt caaaatcctt tgcggaatga tactatttcc
    22981 taatcctcta ctcactacca ttgatgtatt ttgttctaca tataaacccg ctgtatattt
    23041 aggtaaaaca ccttgatttg gcgcaactaa tcctccgata aatggcaatc taacttgccc
    23101 tccatgagca tgtcccgata aaaccaaatc caacctctca ttcgtatatt cctttataaa
    23161 ttcaggccga tgggataata atacattata tttatctggc tgcatttcaa ttttcgcttt
    23221 tttaatttca tctattataa tatttccttc atcacggttt ccagtaacaa actctggatc
    23281 atcaatacct agcaagttta tttcttgctc accctttcga atgcctacat gttcattcct
    23341 taaaacattg acatcatatt tcttcaactc tttttctaaa ttgttatact ttccagacca
    23401 ttgttcatgg tttcctgtca caaaatatac aggatatttc tttacaattt ctcgtattaa
    23461 ttgcatacta acttctgcat cgtatgattt actgtcaatt aaatcacccg taatcgcaat
    23521 aatatctggg tctatacttt ttattttttg aattaacaca tcttgattat cgccaaattt
    23581 tttattgtgt aagtctgaaa tttgtagaat tttataccct ttaaaagagg atggtatttt
    23641 actagaggtt attttaacct cagttatact tattaaattg ttctgtaaat ataaaaagat
    23701 tgtaaaacct actaatatac aaataatcat tacaattcta ttaatcttct ttttcatatt
    23761 ttctccttta cattctcatc aaacacttct atatgctaac tgaattatat tatactttct
    23821 tgaaatttat ttaaattaat aaaataatac agtacatgaa atttcatatg agatatgtca
    23881 tatatattcg ttatattttt acttttacct ttttaataat aagaatacaa aaacgactct
    23941 tatcacacga aaagagtcgt tttctaactt tatactttta atactttcat gttaaaaatg
    24001 cgaaaactaa aatactatta cctaagagcc ttaactataa ctttagttgc ctctgcaatg
    24061 agttgattat cataggttgc ctctttctca tctttactag ataaaattgc gatgataatt
    24121 ggtgctctat tcggaggcca aacgatagca atatcatttc ttgtcccgta actgccagca
    24181 cctgatttat ctccaactac ccagtcagtt ggtacgcctg cacgaataag tttgtcccct
    24241 gtagcatttc ctttcatcca ctctgtaaga attttacgtt tttcagctgg aagtgcattt
    24301 ccgaccgtaa aagctttaag attcgtagca attgctttcg ctgtactagt gtcacgaatg
    24361 tctcctggaa tagcttcgtt taattctgtt tcaaagcgat cagacatagt aacccgatcc
    24421 cccatctttc taagcgcttt ttcatatcct ttaggtccgc ctattttatt aaataaaatg
    24481 ttccctgcag tattatcact tgaacgaaca gcagcctcag caatttctcc tagtttcatt
    24541 ccagtatcta catgtttctc tgtaacaggt gaataatcca ctaagtcttc tttcgtaaat
    24601 gtgattactt catttaatgt atcaattgag ttttgctgta gtaatactcc tgctgctaaa
    24661 gccttgtagg ttgatgcgaa ggcaaatctt tcgttagatc gataagaaat tgtttgattt
    24721 gtaccagtat caatcgcata tacacctact cgagcatcaa attttttctc aagttgagag
    24781 aactctttat gcgtcgcctg atttttatgt ttcacttgtc cagtcttttc tttcgcttca
    24841 acttgcagtg cccctcctgt aaaagcttct aggcttgtaa tacttaaacc taatatacca
    24901 acgcatattc ctatttttag catcctctta tttttcaaaa tcatcattcc ttccaaattt
    24961 ttagcacctt tttatacgta cacataatag acaatcaccg aaccaaattt gttccataaa
    25021 ctaaaaaaat tatagctagt atgagagtta ttaacaaaat aagagatagt agctacgacg
    25081 cctctatctc ctatttcata tttcattttt tacttataaa tggctttata tgaacaggtt
    25141 ctttccctga gaaaagtact ctaattttgc ttagaactac ctgtactagt agcttcacca
    25201 tgtatacgtt gctgttttaa atcaaagtat acatctaat
//
