LOCUS       NZ_MOOL01000024        10330 bp    DNA     linear   CON 03-APR-2024
DEFINITION  Bacillus thuringiensis serovar xiaguangiensis strain BGSC 4BN1
            contig000024, whole genome shotgun sequence.
ACCESSION   NZ_MOOL01000024
VERSION     NZ_MOOL01000024.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis serovar xiaguangiensis
  ORGANISM  Bacillus thuringiensis serovar xiaguangiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 145281)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Comparative genomics of Bacillus thuringiensis reveals a path to
            pathogens against multiple invertebrate hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 145281)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, 1st Shizishan,
            Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.5.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002147905.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 02:08:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,596
            CDSs (total)                      :: 6,434
            Genes (coding)                    :: 6,198
            CDSs (with protein)               :: 6,198
            Genes (RNA)                       :: 162
            rRNAs                             :: 15, 15, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 1 (5S, 23S)
            partial rRNAs                     :: 15, 12 (16S, 23S)
            tRNAs                             :: 114
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 95 of 236
            Pseudo Genes (incomplete)         :: 153 of 236
            Pseudo Genes (internal stop)      :: 70 of 236
            Pseudo Genes (multiple problems)  :: 63 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:39:27
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 27473
            Orig. end                         :: 37803
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOOL01000024.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10330
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10330
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10330
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5330](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5330)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(350..1114)
                     /locus_tag="BK733_RS06900"
                     /old_locus_tag="BK733_06560"
     CDS             complement(350..1114)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732175.1"
                     /locus_tag="BK733_RS06900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06560"
                     /product="DUF3959 family protein"
                     /protein_id="WP_088020273.1"
                     /transl_table=11
                     /translation="MKKLDVLLMLLSGLFPIAGLTKQIPLEQSIYIGGLLFFTSFGSYF
                     AKKMYSRICSWIAYAPFITLLLVIWHQDISTSSIIANAKIAACVALIPCLFRFRTYGLT
                     LGLFSLWAALLWDIKEVQSLVILERITSLMTSHYVYILLLIGGLVLGGLLAMFIHRKEK
                     DNKENKNLLGPKKKRKRLSIKIRLPRLPKLKMKLFKFGGKESKSKMPEKIHEYKYEETV
                     ATYEMTEQIDPYKENAVQGQTRMERRRNRYNA"
     gene            complement(1321..3708)
                     /locus_tag="BK733_RS06905"
                     /old_locus_tag="BK733_06565"
     CDS             complement(1321..3708)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736192.1"
                     /locus_tag="BK733_RS06905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06565"
                     /product="RNaseH domain-containing protein"
                     /protein_id="WP_078993196.1"
                     /transl_table=11
                     /translation="MKKLRLLTFENIVEPLLNEKVSFIYFPIEWLDIVEIHYKTFLLTS
                     KLKRLNERLYDMFSDILFIQHNPYVLNENTPWIVSKEPIRQEQLDYIFQSWYEIIHDWK
                     PNRLVEPPKYEWQSDLISNLPVLHDNETYSKWVPALISHIFCERPIYLENTNEEEIYFS
                     PLRSQNICEAMSGPIKDEKTQDFFSYVYRFECITRGGENAPLLNISIGIRRFYQEYNHP
                     DIPLLLRRKRGMILISTSEFASNNKKIRFVKLKIQQATTGMKWIKIFRNLKDDFQIGGE
                     VDLDHILQYPKDYIVGENKRVLFPYNERIYKVQGTKIEPGITVKEREYLFKEFQRKFSY
                     FTILPECKKVATNNKNELFPLFAPKGLETLILEVWSENIALEIEQALLDSEIVLTKVGE
                     NSYVLNTDFPVLLKIVRYNIEKVLQNPYKMQYCQKYKTNLVDDVKKAVYATAGKRSDAT
                     LALIAMKNCDGREKIDPKQIIREGFARTNRISTFINLFIGQNVSRKTIVNSIFSLLEQK
                     GFLRRSWNKINLPCTYVSLSIERISKFDFLPIFSKIKGKEILYKLYGSTEWQTIDYVLL
                     NINKHNAFLPQPSKRNDMGALFKQYVFETLMEIVQYAKEQNEQVYFIVDANLRKYWIKE
                     LQNKEINIDILPDIVPEVLKVPNLNIIRINTGFDVPSYGVIESDDALDSIGLYADQKGM
                     YYSTGEYSLNCSGIFQRYILEILPLGVKPVERDYIAKMIHYMCCNSSMLSKKNVHMTYS
                     VHMEKVMKTYITDIDAREFKEFDDELDVDVVKVEKKDSITLL"
     misc_feature    complement(3700..3713)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.3"
                     /tool="antismash"
     gene            complement(4020..4505)
                     /locus_tag="BK733_RS06910"
                     /old_locus_tag="BK733_06570"
     CDS             complement(4020..4505)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124614.1"
                     /locus_tag="BK733_RS06910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06570"
                     /product="hypothetical protein"
                     /protein_id="WP_002041181.1"
                     /transl_table=11
                     /translation="MNMIELKSIIHSYKLKRKIAKDLYGKRDELTLLLNEFNKMKCTVT
                     SEKKKNNILSRLQLIYQNMKLDKQYPLSVAFDSKLLERLEKEPLHTIEDGVTCLHLMLD
                     VNYEKIKQYGSSTSRSFVPLSQSSICLADCICLTGVVVGLLGAISFGGLLLSICSIT"
     misc_feature    4537..4555
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.87"
                     /tool="antismash"
     gene            complement(4715..>4851)
                     /locus_tag="BK733_RS06915"
                     /pseudo=""
     CDS             complement(4715..>4851)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016077325.1"
                     /locus_tag="BK733_RS06915"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="site-specific integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="YMYVPTKNIAIIIMDIFNHSSERVTIRYIGVIKR"
     gene            complement(5001..5330)
                     /locus_tag="BK733_RS06920"
                     /old_locus_tag="BK733_06575"
     CDS             complement(5001..5330)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071363.1"
                     /locus_tag="BK733_RS06920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06575"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_048564718.1"
                     /sec_met_domain="TIGR03601 (E-value: 2.1e-40, bitscore:
                     130.2, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQALSLNDYRSGNIVYWDQQNQYPYYYIEDAARRCGGC
                     GRCGGCGGGRCGGFRCGGFRCIGCFGCFGCGGCGGCGGCGGCSNCFDGFSGTTDTTGTI
                     ITYEY"
     gene            complement(5879..6142)
                     /locus_tag="BK733_RS06925"
                     /old_locus_tag="BK733_06580"
     CDS             complement(5879..6142)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101034.1"
                     /locus_tag="BK733_RS06925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06580"
                     /product="DUF3937 domain-containing protein"
                     /protein_id="WP_002082716.1"
                     /transl_table=11
                     /translation="MNFERCYMFTNKKLIRFGLSLFVFLGIINFTISYFQTYLETAADI
                     KWVIPEIWKTILLDVPQGILVLLGAIALYDFTKEASQKDASI"
     gene            complement(6540..7229)
                     /gene="tenA"
                     /locus_tag="BK733_RS06935"
                     /old_locus_tag="BK733_06585"
     CDS             complement(6540..7229)
                     /EC_number="3.5.99.2"
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="BK733_RS06935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06585"
                     /product="thiaminase II"
                     /protein_id="WP_000144507.1"
                     /transl_table=11
                     /translation="MTFSQSLRKEVDSIWEASFNHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITKEEKDNFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGEHLKECQPEEPIYNEWISAY
                     GSDWFRTLVEEQITRLDTIAENVTEADRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDK
                     DVKDVIG"
     regulatory      complement(7357..7462)
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     misc_feature    7501..7513
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     gene            complement(7536..8450)
                     /locus_tag="BK733_RS06940"
                     /old_locus_tag="BK733_06590"
     CDS             complement(7536..8450)
                     /EC_number="6.3.2.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="BK733_RS06940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06590"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_063549318.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 2.1e-23,
                     bitscore: 76.0, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 2.6e-15, bitscore:
                     49.9, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 2.9e-10, bitscore: 33.1,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 1.7e-54,
                     bitscore: 177.5, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 3.9e-07, bitscore:
                     22.9, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRY
                     EGVETPDWIELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVF
                     EWDSEVVIEKYIKGDEITCSILDGKQLPIVSIRHAAEFFDYNAKYDDTSTVEEVIELPA
                     EINERVNKASLACYKALKCSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAG
                     ISYSKLLDMIIETSLKVRNEEGF"
     gene            complement(8455..9903)
                     /locus_tag="BK733_RS06945"
                     /old_locus_tag="BK733_06595"
     CDS             complement(8455..9903)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-469). E-value:
                     5.7e-30. Score: 96.6. Matches aSDomain:
                     nrpspksdomains_BK733_RS06945_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="BK733_RS06945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_06595"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_000471611.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.4e-29, bitscore:
                     96.5, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYLKKMIMKGHLLGDQKIPSTRELSEL
                     LSVSRNTVLAAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKNKLNTVTALADE
                     LDLMKHGVRWEKGMIVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYRPL
                     MNYLLHYMEMKGVDISNKDILITNGFTEGLDIVLSSLSKKSGRVICENPTHHAALKLFR
                     LHGLEVHGIDMHEDGIDTNEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYNVPIIEDGFNEELRYSGSHLAPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQY
                     IPFKRMTGDGGLHLFIELEEHMNARTLLQKCYEKGVTFSPGDVFYSDGEGANTFRLGFS
                     RLKEEEIVRGIKIIGDTLKNENWS"
     aSDomain        complement(8497..9468)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS06945_Aminotran_1_2.1"
                     /evalue="5.70E-30"
                     /label="BK733_RS06945_Aminotran_1_2.1"
                     /locus_tag="BK733_RS06945"
                     /protein_end="469"
                     /protein_start="145"
                     /score="96.6"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYRPLMNYLLHYMEMKGVDISNKDILITNGFT
                     EGLDIVLSSLSKKSGRVICENPTHHAALKLFRLHGLEVHGIDMHEDGIDTNEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYNVPIIEDGFNEELRYSGSHLAPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQYIPFKRMTGDGGLHLFIELEEHMNARTL
                     LQKCYEKGVTFSPGDVFYSDGEGANTFRLGFSRLKEEEIVRGI"
     misc_feature    complement(9979..9993)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 17.35"
                     /tool="antismash"
ORIGIN
        1 ttgcttgttg caacaaaatt attgctgcca tacttaataa taatgcaatg accaatgtca
       61 ttccggcacg aggtgtaaac gccttttccc ctgctttagt tggaataaca ctatttgtcc
      121 cccaattacc tccgaaagcc caatatacat gtaaaaaact aactaagaat aatatacaag
      181 cagatatata tgtaatgata agcattttat cctcccctta tttcattttg gttcccacct
      241 ataaaatatg ctgttccccc cacattagca accttcattt aattacataa aaaaaaccgt
      301 aatgatttat caatattaag ataagtcatt acggggttat taaacctcat tatgcattat
      361 acctatttct tcggcgttcc atccttgttt gaccttgaac tgcattttct ttgtatggat
      421 ctatttgctc tgtcatttca tatgttgcaa ctgtttcttc gtacttatac tcatggatct
      481 tttctggcat tttactttta gattctttcc caccaaactt aaatagtttc atctttaatt
      541 tcggcaacct tggaagtcga atcttaattg ataatctttt gcgtttcttt tttggtccaa
      601 gtagattttt attttcttta ttatctttct ctttgcggtg aatgaacatc gcaagtaatc
      661 ctcctaatac aagtcctcca attaatagta atatatatac ataatggctt gtcattaaac
      721 tcgttatgcg ttcaagtatg actaacgact gtacttcttt tatatcccat agtaaagcgg
      781 cccataatga gaataagccc aaagtaagtc catatgtacg aaaacggaat aaacatggaa
      841 ttaacgcgac acatgcagca attttcgcat ttgctatgat tgaacttgtt gaaatatcct
      901 gatgccaaat aactaacagt aatgttataa atggcgcata cgctatccag ctacatatac
      961 gtgaatacat ctttttagca aaataactac cgaaactagt aaaaaataaa agccctccaa
     1021 tatatataga ttgttctaga gggatttgct ttgtaagtcc tgcaattgga aagaggccgc
     1081 tcaatagcat taagagcaca tctaatttct tcacaattcc ccattccttt caaatttttt
     1141 caattaataa aaatcttttt ccatatgata tagtttttgt atttctaaaa aacatattaa
     1201 tcctgtctgc acataaagta aatatgtaaa cgaaaagata cttgacaaag gatatcctta
     1261 tttacataaa aaaatgacaa atcctgatcg atataacgag caggatttgt ttactagtat
     1321 ttaaagtaga gtaattgaat ctttcttttc tacctttact acatctacat ccaattcatc
     1381 atcaaattct ttaaactctc ttgcatctat gtcagtaata taagtcttca ttactttttc
     1441 catatgtacg gaatatgtca tgtgtacatt ctttttcgaa agcatactag aattacagca
     1501 catgtaatgt atcattttag cgatataatc cctttctaca ggttttacac ccaatggtaa
     1561 aatttcaagt atatatcgct gaaaaatccc actacaatta agtgaatatt caccagtgct
     1621 ataatacatc cctttttgat ctgcatataa gccaatgcta tctagagcat catcagattc
     1681 tattacaccg taacttggta catcaaaacc tgtatttatt ctaataatat ttaaatttgg
     1741 aacctttaac acctctggaa caatatcagg taaaatatct atattaattt ctttattttg
     1801 taactctttt atccaatatt ttctcaaatt tgcatctaca atgaagtaaa cttgttcatt
     1861 ttgttctttt gcatattgta caatttccat taatgtttcg aaaacatatt gtttaaaaag
     1921 agcccccata tcatttctct ttgatggttg tggtaaaaat gcattatgtt tatttatatt
     1981 taataatacg taatcaatcg tttgccactc tgtactccca tataatttat ataaaatttc
     2041 ttttcctttt atttttgaaa aaataggtaa gaaatcgaat ttcgatattc gttcaattga
     2101 taaactaaca tatgtacaag gtaaatttat cttattccaa ctacgcctca aaaatccttt
     2161 ttgttctaat agactaaaaa tactatttac aattgtttta cgagatacat tttgtcctat
     2221 aaataaatta ataaatgttg aaatacggtt tgttcgtgca aatccctctc taatgatttg
     2281 ttttggatca attttttcgc gtccatcaca attcttcatc gcgattaatg ctaatgtcgc
     2341 atcactgcgc ttacccgcgg tagcatagac agctttctta acatcatcta caagatttgt
     2401 tttatatttt tggcaatatt gcattttata tggattttgt aacacctttt caatattgta
     2461 tctcacaatc tttaataaca ctggaaaatc tgtattcaat acataagaat tttcaccaac
     2521 tttagtcaaa acaatttcgc tatcaagtaa tgcttgttct atttccagcg ctatattttc
     2581 agaccaaact tctaatatta atgtctctaa tccttttggt gcaaataaag gaaataattc
     2641 atttttgtta tttgtagcta cctttttaca ttctggaagt attgtgaaat aagagaattt
     2701 acgctgaaat tctttaaata aatactctct ttcctttact gttatacccg gttctatttt
     2761 agtaccttga actttataaa ttctctcatt gtatggaaat aacacccttt tattttctcc
     2821 tacaatataa tctttcggat attggagaat atggtctaaa tccacctctc cccctatttg
     2881 aaaatcatct tttaaatttc taaatatctt aatccatttc atgccggttg tagcttgttg
     2941 tatttttagt tttacaaacc gtattttttt attatttgac gcaaactctg aggtagaaat
     3001 caatatcatg ccccgttttc gtcttaaaag taaagggata tctggatgat tatattcttg
     3061 ataaaatctc cgaatcccta ttgaaatatt taataatgga gcgttctcac caccgcgggt
     3121 tatgcattcg aatcgatata cataggagaa aaaatcttgt gttttttcat cttttatcgg
     3181 ccctgacatc gcctcacaaa tattttgtga tctaagaggg gaaaaataga tttcttcttc
     3241 atttgtattt tctaaatata taggacgctc acaaaaaata tgtgagatta aagcgggcac
     3301 ccacttagaa tacgtttcat tatcatgtag tactggcaaa ttagaaatca aatcggattg
     3361 ccattcatat tttggtggtt ctactaatct atttggtttc caatcatgaa taatctcata
     3421 ccaactttga aaaatataat cgagttgctc ttgtctaata ggttctttcg atacaatcca
     3481 tggtgtattt tcatttaata cgtacgggtt atgctgaata aacaatatat ccgaaaacat
     3541 atcatacaat ctttcattca aacgtttcaa cttactcgtt aataaaaatg tcttataatg
     3601 tatctctacg atgtccagcc attcaatagg aaagtatata aatgatacct tttcatttaa
     3661 aaggggttct actatatttt caaatgttag cagccttaat tttttcataa aatgattcct
     3721 tcctttcttc agttgtcttg attatcaaaa agctagaaca atagttactt aatctaatcc
     3781 atttaaacca agtatttaaa agaaacgtaa aaactgttta tctacataaa ttattatcaa
     3841 accgactctt ttaaaaattc aaagtaatta tcatgtataa cacctctatt atatttgaat
     3901 ttcgatacat tcgaattata cattacactt aatgcattgt acttatcatt tacaatatct
     3961 ttacaaaaaa accaaaaaag aacaccttaa ttcggtgccc tttttacaaa tatcaacctt
     4021 tatgtaattg aacatataga taacagtaat cccccaaatg aaattgctcc tagtaaacct
     4081 actacaactc ctgttaaaca aatacaatca gcaagacaaa tagaagactg cgataatgga
     4141 acaaatgacc tacttgtact cgaaccatat tgttttattt tctcataatt cacatctaac
     4201 attagatgta aacatgttac accatcctca atagtatgta gaggttcttt ttctaatcgc
     4261 tccaataatt tactatcaaa agcaactgat agaggatact gcttatctaa tttcatattt
     4321 tgataaatta gttgcaaacg agataatata ttgtttttct tcttttcaga tgttactgta
     4381 catttcattt tattaaattc atttaataac agtgtcaatt catctctttt tccgtataaa
     4441 tctttagcta tttttctctt tagtttatac gaatggataa tagattttag ttcaatcata
     4501 ttcatacact tcctaattac atattattta cccgattatt ttatagataa acagtacttt
     4561 ttaatcttat ttaaattaca ctcaagcatc ttcatttact atcgattaac attacattaa
     4621 ccttacaaag ttgtaatgaa ttcattagct agaaggaatc aatccctaaa ccagacagat
     4681 atttgatgat aaaaccataa gaaaaaagca atgattagat tttaaatcta gtcattgctt
     4741 tatccatcac ctcttgatta cacctatata tcttattgtt actctttcac ttgaatgatt
     4801 gaatatatcc attataataa tggctatatt ctttgtcggc acgtacatat ataaaactaa
     4861 tgttttttaa ttgataaggt gaaactttaa ttagccctca ccaattgggc ttttatgggt
     4921 agtccgccac ctaacttctc tttgctcccg ctgaattttt ttagggtctt actgccctgc
     4981 aaatagcagg ataaatgcaa tcaatattca tacgttataa tagtaccagt agtatcagta
     5041 gtaccactaa aaccatcaaa acagttagag caaccaccac aacctccaca acctccacaa
     5101 cctccgcaac caaaacaacc gaagcaacca atacaacgga atccaccaca acggaatccg
     5161 ccacaacgac ctccaccaca accaccacaa cgaccacaac cgccacagcg acgagcagca
     5221 tcttcaatat agtaatatgg atattggttt tgctggtccc aatagacaat atttccagac
     5281 cggtaatcat taagacttaa cgcttgtagt tcttgttgaa actgattcat tttcataacc
     5341 tccgtttata aaatacaacc tcattaatgc ttccctatat ttctgttcgt tacatctaag
     5401 taaaaatagt gctataaact aagttcaata cgtacaccaa caaagtatga cttatcacta
     5461 aatgatgcac cttgttcata tacctatttt tgctatgggc tcatttttat aaagtgaaac
     5521 tttaatcagt ggggggagtt catcccccgc tgattattat ccctcaccaa tcaggctttt
     5581 acgaacagcc cgttaatgcg ggataaataa aaattaaaaa atatgaattc tagcgtcata
     5641 tcttcatacc tactaaattc aattaatttc tcaataatta tgttgtcata tcctatatat
     5701 ttccgtctgc cgcttagatt tttctgtact gtaaatgcct ttcgctataa cacctccaag
     5761 taactggttt ctatgacgga taagaaattc ctacaaagaa aaaagccctt attagggctt
     5821 ttcattctat ttctccagca aaactctcta tgaaattcct aatagaaaaa agacacactt
     5881 agattgatgc gtctttttgt gatgcctctt ttgtgaaatc atataaagca attgcgccta
     5941 aaagaacaag tataccttga ggaacatcta gtaaaatagt tttccaaatt tctggaatta
     6001 cccatttaat atctgctgct gtttcaagat atgtttggaa atagctgatt gtaaaattaa
     6061 ttatccctaa aaatacaaac aacgataaac caaatcgaat taatttctta tttgtaaaca
     6121 tataacacct ctcaaaattc attggatatt cctgcttttg cttctttata tttccatatc
     6181 tttatatcta attttaacac ataaagaact caaaaaaata cgatatgcat atttgcatat
     6241 cgtattaaca acactccatg ataatttaga aattcctgca aagaaaaaac aactccgtta
     6301 cagctaacag ctcgggtttc ttttcttatt taacttcata ccaccaacat ttacgttctt
     6361 tgattgtaaa ataaatgtga cagaagttta tccagttaat gcgtagacaa gtaaatgagc
     6421 taaattatcg atattccatt tcgccatatc cgtagcacta atgactttac gcaaatttga
     6481 caagaaaaaa tatatagata gatattatcc atacctatct ataacattac ttctattcct
     6541 tatccaatta cgtcttttac gtctttatcc actggccact tttctaatgt ataagccatt
     6601 tcccaaaatg aatattcata ttgactgcta ataataaaat gctgtttcat acgtttgcgg
     6661 tctgcctctg ttacattttc agcgatagta tctaaacgtg taatttgttc ttcaactaac
     6721 gtacggaacc aatcagatcc ataagcagaa atccattcgt tataaattgg ctcttccggc
     6781 tggcactctt ttaagtgttc gccaatttca taatacaacc aatagcaagg taaaattgct
     6841 gcaatgatat ctcctaaatg cccttcatac gcagcacgat acatatgtga agtatacgca
     6901 tatgcagttg gcgcaggaat aaaattatct ttttcttctt ttgttatccc tagcttttta
     6961 gcaaaattct catgtaaaga taactcggcc tcatatgtat tttgagcatg atgtgccata
     7021 cgagctgtcg tctctaattc aagagctttt gcagctccta aagtttgtac tctagcaaaa
     7081 tgactcaaat aatatgaatc ttgaagcaca taatagcgaa aactagctaa atctaatgtt
     7141 ccttcaccaa gtttttttac aaaaggatgg ttaaaactcg cttcccaaat tgaatctact
     7201 tctttacgta atgattgtga aaaagtcatt taaatccctc caataatgta taaaatataa
     7261 gtatttatcc acaattatta aagctataaa ataaaaaaac tttctttaca ggcgtaaaga
     7321 aagtgaaatt gacagtattt atttactcat taaatagcaa cgtctttccc acttccctac
     7381 gctggcatta accagatcag gtactaagag tctcaaaatc acatttgatc tcagcctata
     7441 aaaggccccc ctagtggata aatattttgt ttttataatg tatccatcat agcatacttc
     7501 gtgaaactgt caaaagaatt agttatataa tctccttaaa acccttcttc atttctaact
     7561 tttaatgatg tttcaataat catatctaat agtttgctat aactaattcc cgctgcttct
     7621 gcacttttgg gcaataggct tgattgtgtc atccctggta atgtattaat ttccattaca
     7681 tatggaattc cgtccttcac catcatatca actcttgcat aaacactaca ttttaatgct
     7741 ttataacaag ccagtgaagc tttatttaca cgttcattaa tttctgctgg aagttcgata
     7801 acttcctcaa ctgtactagt atcatcatat tttgcattgt aatcaaagaa ctcggctgca
     7861 tgtcgaattg aaactatagg taactgtttt ccatccagaa tagaacatgt aatttcatca
     7921 ccttttatat acttctcaat tactacttca gaatcccatt cgaatacagt ttctagcatt
     7981 gagatcaatt catttttatt gtaaacgatt ttcaccccga cactcgatcc gccggagttt
     8041 ggttttacca ctaatggaaa tcctaactta tctaactcat caaaatttag atcctccatt
     8101 tttgtaagtt caatccaatc tggtgtttct acaccttcat aacgtaaaat ctttttcgaa
     8161 atatttttat ccatacatat actactagat aacatattac ttccgctata aggaatccct
     8221 aaactctcca gtgttccttg aactgtccca tcttctccgt attttccgtg taatgcgagc
     8281 aatgcaaagt caatgtcttt cgctttttca attaaatcca ttttttcatt taatgtaatt
     8341 gggactattt catacttatt tttatctaaa tgagcaatca tttcattccc ggtcataatt
     8401 gaaacttgtt tttcagacga taccccgccc ataataacac cgattttcat tttctcaact
     8461 ccaattttca ttttttaatg tatcaccaat tattttaatt ccccgaacta tttcttcttc
     8521 tttcaagcgc gaaaacccta atcggaaagt gttcgctcct tctccatcag agtaaaaaac
     8581 atctccaggt gaaaacgtaa cgcctttctc ataacacttt tgtaaaagtg tgcgggcgtt
     8641 catatgctct tctagctcta taaataaatg cagtccacca tctccagtca ttcttttgaa
     8701 aggaatgtac tggttacatg ccccgacagc taactcatat tttttcttat aaacagatct
     8761 tgcctttttt aaatattttt caaaatatcc ctcatgtaaa tattgaaaca gaacagcttg
     8821 atctagcgta gatgtgtgaa tcgttcttgc tctttttacg ctttccaaat aatgaatcag
     8881 ctctttatct gcaataatcc accctacacg taaaccaggg aaaagtacct ttgaaaagct
     8941 actaatataa atcacattat tgccagcacc agcaaaagtt aataatggag ctaaatgtga
     9001 acctgaataa cgtaattctt cattaaatcc gtcctctata ataggaacgt tgtattttga
     9061 gaataacctc atcaattccg ttcttttctc tgaactcgta acaataccgg ttggattatg
     9121 ataagaagga attaaataag caaaatcaaa atctttttca cgcaaacttt tttcaacttc
     9181 attcgtatca ataccatcct catgcatatc aatcccatga acttcaagcc catgtaaacg
     9241 gaaaagctta agtgcagcgt ggtgagttgg attctcacaa atgacacgcc cggatttttt
     9301 tgataaagaa gagagtacaa tatccaaccc ttctgtaaat ccatttgtaa ttagaatatc
     9361 tttattcgaa atgtctacac ctttcatttc catgtaatga agtaaataat tcattaacgg
     9421 tctatatccc tttgcatatc cgtagttcaa tacgatgtcc ccttcaatgg acatacgagt
     9481 aagaaaagct cttttgaaat tttcaacatc aaatagcttc tcatccgggg ctatactatt
     9541 aaaaacaatc attccctttt cccaacgaac accatgtttc attaaatcta attcgtccgc
     9601 taatgcggtc actgtattaa gtttattttt ccaatctatt tcaacagacg aagtgttaga
     9661 tatatccacc ttcgcaacaa agttcccttt tcctttaact gcataaatga gtccttcttg
     9721 ctctaaatct gcataagcag cgagtacagt gtttctactc acacttagta attcacttag
     9781 ttcccttgtt gatgggattt tttgatcccc gagcaaatgc cctttcataa tcatcttttt
     9841 caaatagtct tttaattgaa tataaacggg acgatcttta acaactttaa aatccttaaa
     9901 caattcctta ccccctgcaa tcatttttgc atatttattt cattctcaaa aggtccacga
     9961 catatgtatt ttttataaag ggcggtacgg tcttacaaat cttttcgata taagttaatt
    10021 acatctttta aagctgcagc tgcagcgcct cgtggattag aagccccacc aattgtagta
    10081 acttgtccca tcgtatctgt aaagaacgta attccttttt cgctcccatt tacttttgct
    10141 aacggatgat ctttttctat ttcgcacggt tcaacattta aattcacatt gccaccctca
    10201 tccttatata ttgatgctat taatttaata attttatgtt gtttcttagc attttgtatt
    10261 tgctgttttg taacgtgctc gattcctttt atatgtatat cagtaagtgt tttctctgtt
    10321 tccattaagc
//
