LOCUS       NZ_MOOL01000022        10267 bp    DNA     linear   CON 03-APR-2024
DEFINITION  Bacillus thuringiensis serovar xiaguangiensis strain BGSC 4BN1
            contig000022, whole genome shotgun sequence.
ACCESSION   NZ_MOOL01000022
VERSION     NZ_MOOL01000022.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis serovar xiaguangiensis
  ORGANISM  Bacillus thuringiensis serovar xiaguangiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 166777)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Comparative genomics of Bacillus thuringiensis reveals a path to
            pathogens against multiple invertebrate hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 166777)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, 1st Shizishan,
            Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.5.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002147905.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 02:08:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,596
            CDSs (total)                      :: 6,434
            Genes (coding)                    :: 6,198
            CDSs (with protein)               :: 6,198
            Genes (RNA)                       :: 162
            rRNAs                             :: 15, 15, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 1 (5S, 23S)
            partial rRNAs                     :: 15, 12 (16S, 23S)
            tRNAs                             :: 114
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 95 of 236
            Pseudo Genes (incomplete)         :: 153 of 236
            Pseudo Genes (internal stop)      :: 70 of 236
            Pseudo Genes (multiple problems)  :: 63 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:39:27
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 30300
            Orig. end                         :: 40567
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOOL01000022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10267
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10267
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10267
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5267](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5267
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            978..2339
                     /locus_tag="BK733_RS05890"
                     /old_locus_tag="BK733_05605"
     CDS             978..2339
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017763222.1"
                     /locus_tag="BK733_RS05890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_05605"
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_088020201.1"
                     /transl_table=11
                     /translation="MNGKRKIFTCISIVGIGLASFSNSSFAASVTDNSIQNSIPIVNQQ
                     VAAAKEMKPFPQQVNYAGVIKPNHVTQESLNASVRSYYDNWKKKYLKNDLSSLPGGYYV
                     KGEITGDADGFKPLGTSEGQGYGMIITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNL
                     MGWVVADSKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGTVNYLKEAQDMITKGIKAS
                     NVTNNNRLNLGDWDSKSSLDTRPSDWMMSHLRAFYEFTGDKTWLTVINNLYDVYTQFSN
                     KYSPNTGLISDFVVKNPPQPAPKDFLDESEYTNAYYYNASRVPLRIVMDYAMYGEKRSK
                     VISDKVSSWIQNKTNGNPSKIVDGYQLNGSNIGSYPTAVFVSPFIAASITSSNKQKWVN
                     SGWDWMKNKRESYFSDSYNLLTMLFITGNWWKPAPDDKKTQNLINDAIYEGYDN"
     gene            2502..3425
                     /locus_tag="BK733_RS05895"
                     /old_locus_tag="BK733_05610"
     CDS             2502..3425
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="BK733_RS05895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_05610"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_088020203.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATKPLTEEITTYYPEIHGAEGLGPIHVPGNILSIPIYNF
                     GSIAAILIKYGKDLTIVDVGRSTSLAIAFNLWNDLMLNVKGIYFMGGVFLEVGNVTPLA
                     EANVYGDPIASKIVFHQAKNLLIFPLNVTNKAVLTPNVFNYIQANAKNPFHMIMKPMYD
                     YYLSAYQKLNPSMEGPLLHDVVAISGLVNPSFFEFVHRTVNVDTYGDTKGQTFADFRPN
                     SKSEGARIALEIDAEKFIQDFIKIML"
     gene            3502..3960
                     /locus_tag="BK733_RS05900"
                     /old_locus_tag="BK733_05615"
     CDS             3502..3960
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="BK733_RS05900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_05615"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_001102673.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRDNWEDALKLQVKEDQRKFVPAVAVSLAKVYIKPDG
                     DNVEYIPFAIYDGDLMVGFVMHAVVIETSDMYWINGFIIDQSQQGNGYGKAALQESINI
                     IKNTFKACKEIRLTVHKDNISAKKLYERYGFISLGQEYDGEQVYRLFV"
     gene            3991..4338
                     /gene="cutA"
                     /locus_tag="BK733_RS05905"
                     /old_locus_tag="BK733_05620"
     CDS             3991..4338
                     /GO_process="GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="cutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160776.1"
                     /locus_tag="BK733_RS05905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_05620"
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="WP_001160792.1"
                     /transl_table=11
                     /translation="MQFTEVKIEVFIPEEYIETLRDELNKIGACKTGEYDHCLSYSSVK
                     GYWRPLEEASPFNGEIGQICEGQECKVEIKCKRELVKDVLEVINDIHPYETPMIYIIPI
                     LNDYFEKLYIK"
     gene            4355..4801
                     /locus_tag="BK733_RS05910"
                     /old_locus_tag="BK733_05625"
     CDS             4355..4801
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="BK733_RS05910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_05625"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_000836831.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAVMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LKRELREELGVNCSIQQFLGVIENQWQDKEVLHHEINHIFEVESQDLHTDLTPKSSESH
                     LAFHWIAYNKEALNHYEIMPMPLVKELLESKLNDELLNCWISNF"
     gene            5001..5267
                     /locus_tag="BK733_RS05915"
                     /old_locus_tag="BK733_05630"
     CDS             5001..5267
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071381.1"
                     /locus_tag="BK733_RS05915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_05630"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001071389.1"
                     /sec_met_domain="TIGR03601 (E-value: 3.2e-49, bitscore:
                     158.9, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQSLNLNDYQTGNVVYWDQQQSQYPYYYIQDDARRCGG
                     CGGCGGRCGGCGGRCGGCAGRCGGCIGCAGCFSCFNCWNWWII"
     gene            5369..6385
                     /locus_tag="BK733_RS05920"
                     /old_locus_tag="BK733_05635"
     CDS             5369..6385
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="BK733_RS05920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_05635"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_063547443.1"
                     /transl_table=11
                     /translation="MYTYDKLISWVEDIKEKNHSSATALCIIKDNKIVLEHYSGYHSNP
                     STTKRLTASSQFNVASARKSYLGLMIAYALYEGKINSIDDEAIKYFKDFDPALLSKTTI
                     RHLVTHSHGLEETNDRTIFREFAPGQSWAYRDINVRMMTRLIYQLYNKSFPELLKERVF
                     KPANFQETGWRVQRDENLVDVVNNPNEDAISEIGTVDDGTEKNLFVSAREFAQWGNLHL
                     NQGGIDGKQIVPKEVIKIATSLQSPTYINKELPQNGLFWFIQNEPAQLSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSNGNRA"
     gene            6417..6560
                     /locus_tag="BK733_RS34580"
                     /old_locus_tag="BK733_05640"
     CDS             6417..6560
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="BK733_RS34580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_05640"
                     /product="hypothetical protein"
                     /protein_id="WP_000653680.1"
                     /transl_table=11
                     /translation="MKDKVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            6840..8048
                     /locus_tag="BK733_RS05930"
                     /old_locus_tag="BK733_05645"
     CDS             6840..8048
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="BK733_RS05930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_05645"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_063547444.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAAKLQKKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLIDKLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNAVTETLHNDYTPREMMRTKQVYLLFFMLFTS
                     CMGGLYLISMVKDIGVQLVGLSAATAANAVAMIAIFNTVGRIILGTLSDKIGRMKIVSA
                     TFIIIGLSVFTLSFIPLNYEIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGAILGGFQPTFIIIGVLSVISFIISILIRPPNVEKKKELKHLHR
                     KVA"
     gene            8179..8604
                     /locus_tag="BK733_RS05935"
                     /old_locus_tag="BK733_05650"
     CDS             8179..8604
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706722.1"
                     /locus_tag="BK733_RS05935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_05650"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_000706912.1"
                     /transl_table=11
                     /translation="MKIVQQWVQEDSDYIREKVIEYNQKYLAGEEKTPSEKVSFIVRNE
                     KEEIVGGVTAITFWHHIHVDFLWVSEEYRHEGYGSKLIKLIEEFAIEKECRLINLDTFS
                     FQAPDFYKKHGYKVIGVSEDHPKGHNHYYLEKRLKSI"
     misc_feature    complement(8785..8803)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.4"
                     /tool="antismash"
     gene            8884..9327
                     /locus_tag="BK733_RS05940"
                     /old_locus_tag="BK733_05655"
     CDS             8884..9327
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="BK733_RS05940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_05655"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_000757628.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDKTNEKILMVKNKGENGSYYTLPGGAVKLGETL
                     EEAVIREVKEETGLHINVNGICSISEAFFEERDHHAIFFNFLGEIIGGETYISRPKEIE
                     EITWMELHIAAPYLRIPEHLLDLLQKKETVPYFFNGTIVHQSS"
     misc_feature    10114..10132
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.64"
                     /tool="antismash"
ORIGIN
        1 cagcaattcg tctggggctt gaatcttata cgcctgaaca ccttacttta ctacgcttat
       61 taatcgcctc atttatactt ttgttatttt catttatata caaactacga ctaccggatt
      121 taaaggatat cccagcaatt tttatatttg gtgctttagg gtttactatt tatcacatcg
      181 cattaaatta cggcgaaaaa acagtaaatg ctggatctgc aagtttaatt atttcagtca
      241 ccccaatagt gacggcaatt cttgcttctg tttttttgaa tgacaaaatg aaattaaatg
      301 gttggcttgg tggtgtaatt agttttgcag gaattgcctt aatttcattt agccagggcg
      361 atgctattca attgaatagc gggggattat ttatattatt agcggcgatt tcagaaagtc
      421 tatttttcgt tttccaaaca tcttacttaa aaaaatacgg cttcttacca tttaccatat
      481 atacaatttt gtctagtact gtatgtatgc ttattttctt acccggagtt tatcaagaaa
      541 tactaacagc tcctcttgaa gttaacttga gcgttatata tttaggtgtc tttccaacag
      601 tactcccgta tattgcattg gcctatatta tatctcgtgc tggtgcttct gaagcaacaa
      661 gttctctata tttaacgcca ataactgcgt gtttcattgc ttggatatgg ttaggagaag
      721 tcccaacttt agtttcgata attggcggag gaattactat acttggaatt gtgattgctc
      781 atataccagt attaagaaaa gaaaaacata ggaatttagc aaataatcaa tccgtataat
      841 gtaattcaat aatgatttta tgatactttt ttatatagtt tctttcttat gttgataaat
      901 ataatatttt gtatttcagt taaatattgt atgttacatt ttatgtagta agcattttaa
      961 aaggagctga caacataatg aatggaaaaa gaaaaatttt cacatgtatt tctattgtag
     1021 gaatcggact agctagtttt tctaattcta gtttcgcagc aagtgtaacg gacaattcaa
     1081 tacaaaattc tattcccata gttaatcaac aagtagctgc tgcaaaggaa atgaaaccat
     1141 ttccccagca agttaattat gcaggtgtta taaaaccgaa tcatgttaca caggaaagtt
     1201 taaatgcttc tgtaagaagt tactacgata attggaaaaa gaaatatttg aaaaatgatt
     1261 tatcttcttt acctggtggt tattacgtaa aaggagagat tacaggtgat gcggatgggt
     1321 ttaagccact tggaacttca gaaggtcaag gatatgggat gataattaca gtattaatgg
     1381 ctggttatga ttcgaatgct caaaaaatct atgacggttt atttaaaaca gcaagaactt
     1441 ttaaaagctc tcaaaatcct aatttaatgg gatgggttgt cgcagatagt aaaaaagcac
     1501 aaggtcattt tgattctgct actgatgggg atttagatat tgcgtattct cttcttcttg
     1561 ctcataagca gtggggatct aatggaacag ttaattattt gaaagaagca caagacatga
     1621 ttacaaaagg tattaaagct agtaatgtta caaataataa ccgactaaat ttaggagatt
     1681 gggattctaa aagttcactt gatacgagac catctgattg gatgatgtca caccttagag
     1741 cattttatga atttacaggt gataaaactt ggcttactgt tattaataat ttgtacgatg
     1801 tttatacgca atttagtaat aagtactctc caaatacagg acttatttca gatttcgttg
     1861 taaaaaaccc accacaaccc gcacctaaag acttcttaga tgagtcagaa tatacaaatg
     1921 catattatta taatgctagt cgagtacctt taagaattgt aatggactat gcgatgtacg
     1981 gcgagaagcg aagtaaagtc atttctgata aagtctcttc atggattcaa aataaaacga
     2041 atggaaatcc ttctaaaatt gtggatggtt atcaattaaa tggatccaat attggtagtt
     2101 atccaactgc tgtatttgtt tcaccgttta ttgctgcaag tataacgagt agcaataaac
     2161 aaaagtgggt aaatagtggt tgggattgga tgaagaataa gagagaaagt tattttagtg
     2221 atagttataa tttattaact atgttattta ttacaggaaa ttggtggaag cctgcacctg
     2281 atgataaaaa aacacaaaat ctaataaatg atgcaattta tgaaggatac gataattaat
     2341 taaatttgag aatggttact gaagaaagga attagcattc gctagttcct ttttaatgtt
     2401 ttgtaaaaca ttatccattc taaaattcgc atagatatac ttgctttttt catatacata
     2461 gttattgatg actctatgtt tttatgaagg gagagtattt cttggaaaaa gtattatttt
     2521 tcggagaccc aggtattgat gactcatttg ccattatgta tggtttactg catcccgaaa
     2581 ttgaaattgt cggtattgta accggatacg gaaatgttga gcatatacat gctgcacata
     2641 atgcagcgta tattctacag ttagcaaata gacaagacat tcctgtcatt agtggtgcaa
     2701 caaaaccact gacagaagaa atcactacgt actatcctga aattcacgga gcagaaggat
     2761 tagggcccat ccatgttcca ggaaatatat tatctattcc aatatataat ttcggcagta
     2821 ttgctgcgat tcttataaag tacggaaaag atttaacgat tgttgatgta ggaagatcta
     2881 cctcgttagc aattgctttt aatttatgga atgatttaat gctaaatgta aaaggaatct
     2941 atttcatggg tggtgttttt ttagaagtag gaaacgttac gccattagca gaggcaaacg
     3001 tgtatggaga tccgatagca agtaaaattg ttttccatca agcaaagaat ttattaatat
     3061 tccctttaaa tgtaacaaac aaagctgtgt taacaccaaa tgtatttaac tatattcaag
     3121 ctaatgcaaa gaatccattt catatgataa tgaaaccaat gtatgactac tatctatccg
     3181 cataccaaaa acttaatcca tctatggaag gacctttgct acatgatgtg gttgcaataa
     3241 gtggattagt aaatccaagt ttttttgaat ttgtgcatcg tacagtaaat gtagatacat
     3301 acggagatac gaaaggacaa acatttgctg attttcgccc taactctaaa tctgaaggag
     3361 cccgtatagc tttagaaata gatgcagaaa aattcattca agattttata aaaattatgt
     3421 tataagttgc tgtcgaaaaa gggaattatt tcaatgcgtt gaatataaaa atacatcctc
     3481 tagataaagg agcttacaat attgaatgtc cagttaaagg ttgttacgag agacaattgg
     3541 gaagatgcat taaagttaca agttaaagaa gatcaaagaa aatttgtccc cgctgtagca
     3601 gtttcacttg ctaaagtata tataaaacca gatggtgaca atgtagagta cataccattc
     3661 gctatatacg atggtgacct tatggttggt tttgttatgc atgctgttgt aatagagaca
     3721 tcggatatgt attggattaa cggatttatt attgatcaat cgcaacaagg taatggttat
     3781 ggaaaagcag cattacaaga aagtattaat ataataaaaa atacgtttaa ggcgtgtaaa
     3841 gaaattagat taaccgtaca taaagataat atctctgcaa agaaattata tgaacgatat
     3901 ggtttcatat cattaggaca ggagtatgat ggtgagcaag tatatcgttt atttgtttaa
     3961 ctattaggaa gtaaaggtgt gaaatgaaat atgcaattta ctgaagtgaa aattgaagtg
     4021 ttcattcctg aagaatatat tgaaacatta agggatgaat taaataaaat tggagcatgt
     4081 aaaacgggcg aatatgacca ttgtctttcc tatagttcag tgaaaggata ttggagacct
     4141 ttagaagagg catctccttt taatggagaa attggtcaaa tatgcgaagg acaagaatgt
     4201 aaagttgaaa taaagtgtaa acgagaactt gtaaaagatg ttcttgaagt tattaatgat
     4261 attcatccgt atgaaacacc tatgatttat attataccaa tactaaatga ctattttgag
     4321 aaactataca ttaaataact aggaggaata tcaattgaaa agtaaatttc atcatattgt
     4381 acgagcagtc atgataaaag atgaaaaatt attagttgct gaatatatcg gccatcatta
     4441 ttttttacct ggtggccatg ttgaaattgg agaatcagca gagaatgcat taaaaagaga
     4501 gttaagagaa gaacttggag taaactgtag tatacaacaa tttttaggcg tcatagaaaa
     4561 tcaatggcaa gataaagaag ttcttcatca tgaaattaac cacatttttg aagtagagtc
     4621 acaagattta catacggact taacaccgaa atctagcgag tctcatttag catttcactg
     4681 gatagcttat aataaagaag ccttaaatca ttatgaaatc atgccaatgc ctttagtaaa
     4741 ggaattacta gaaagtaagt taaatgatga actcctaaat tgttggatta gtaattttta
     4801 aatcacttta tttatagtaa atacgtccat cgaatgaata taaagaaaat aggctgaaaa
     4861 acaaggtaca tcacctagtg taagttcata ttttgttgat gtacgcattt agtctatagt
     4921 cttgtttcta ctttgtttaa aagaatttat taactttaat atttggacgg aatttacatt
     4981 tataaatgga gggtttcata atgaatcagt ttcaacaaga actacaatca ttaaacctta
     5041 atgattatca aactggcaat gttgtgtatt gggatcaaca acaaagtcaa tatccatact
     5101 attacattca agacgatgca cgtcgttgcg gcggatgtgg cggttgtggt ggacgttgcg
     5161 gtggatgtgg cggtagatgt ggtggttgtg caggtcgttg cggcggatgt ataggatgcg
     5221 caggatgttt cagttgcttt aattgctgga actggtggat tatttaagta tagaaaatcc
     5281 aagttatttt atatgaaaga aaggtgtaaa aactattttg cacttttctt tcatgctgta
     5341 attttgatgt gtattaaggg ggatttattt gtatacatat gataagttga tttcttgggt
     5401 agaggatata aaagaaaaaa atcatagttc tgctacagca ctttgtatta taaaagataa
     5461 taaaatcgta ctagagcatt atagtggtta tcactcaaat ccatctacaa ccaagagatt
     5521 aacagcatct tcacaattta acgttgcttc tgctagaaaa agttatttag gattaatgat
     5581 agcgtatgcg ctttatgagg ggaaaataaa ctctattgat gatgaagcga taaaatattt
     5641 taaagacttt gatcctgcat tgcttagtaa aacgacgata agacatttag taacacattc
     5701 gcacggatta gaagaaacga atgacaggac aatttttcgt gaatttgcac cgggacaatc
     5761 atgggcgtat agagatatta atgtaagaat gatgacacgt cttatttatc agctatataa
     5821 caaaagtttt cctgaattgt taaaggagcg tgtgtttaaa cctgctaatt ttcaagaaac
     5881 aggttggaga gttcagcgag atgaaaattt agttgacgtt gttaataatc caaatgaaga
     5941 cgcaattagt gaaattggta cggtagatga cggtactgaa aaaaatttgt ttgtctcagc
     6001 tagagaattt gcgcagtggg gcaatcttca tttaaatcaa ggtgggatag atggtaaaca
     6061 aattgttcca aaagaagtta taaaaatcgc tacgagtttg cagagtccaa catatataaa
     6121 caaagagcta ccacaaaatg gattgttttg gttcatccaa aatgagcctg cgcaattaag
     6181 cgaacttggt gaacgtgttc caaaagggtc gtatcaaata ttagggatta ctggaccgac
     6241 cattttagta atacctgaat ataatgtagt tgttgcaaaa atgtataata aaagatataa
     6301 ctacggtggt gataattact tatattattt acgtgaattt agtaatttag tcgctgatac
     6361 atttagtaac ggtaataggg cataattact cctataacaa aaaggagtga ttgaaaatga
     6421 aagataaagt gaacaaaaat aaaaagcaaa atataaaaga tgtaaataca gagcaaaatg
     6481 caatttatag cgatcctaaa gatgcagcta atatgcaaac tgtaccgcag ccgaaagatt
     6541 ttgatgaaat cgaatattaa tttttcatcc gactatatga tgaatgaaaa atacgtctta
     6601 agtctcataa catttaagaa attatttgat aacatatata cataagcatt tgaaaatgtc
     6661 ttatcttaac aaagttttct ttagaaatat ctgatataac ctgcaaaagg cacttcttta
     6721 tgaagtgtct ttttgtgtat atgaacttaa ttaccatttt ccgtacagta tcttgcaatt
     6781 tatgatggat atactaactg tgaaaattca tgtcaagata aaagaagggg acagttagta
     6841 tgaaacaaac ttctataaat ccgttactaa ttgttctagg tacaatcatt gttcaaattg
     6901 gccttggaac aatttataca tggagtttat ttaatcaacc ccttgtaagt aagtttggat
     6961 ggaatcttaa ttcagtagcg ataactttct ccataactag tttttcttta tcattctcaa
     7021 ctttatttgc agccaagttg cagaaaaaat taggacttcg aaaacttatt gctactgcag
     7081 gaattgtttt aggactcggc ttaatactta gttcacaagt ttcttcttta ccactattat
     7141 atttattagc tggtgtcgtt gttggttatg cggatggaac agcttatatt acatcactat
     7201 ctaatttaat taaatggttt ccaaatcgga aagggcttat ttcaggtata tctgtatcag
     7261 cgtatggaat gggcagctta atctttagat atataaacgg aaatcttatc gataagcttg
     7321 gtgtatcaca agcattttta tattggggta ttatcgtgtt acttttagtg ttaatcggat
     7381 cgttcttctt acgtgaggca attgtaagta atgctgtaac tgaaacatta cacaatgact
     7441 atactccgcg tgaaatgatg cgaacgaaac aagtatatct gctatttttt atgttattca
     7501 cctcgtgtat gggtggtttg tatttaatca gtatggtaaa agatatcggc gtacaacttg
     7561 ttggacttag cgcagcaacc gctgcaaacg ccgttgctat gattgcgatc tttaatacag
     7621 taggtagaat cattcttggg acgttatcag ataaaatcgg ccgaatgaaa attgtctctg
     7681 caacttttat tattataggt ttgtcagtct ttactttaag ttttattccg ctaaattacg
     7741 aaatctattt tgcttgtgta gcaagtgtcg ccttttgctt cggtggtaat ataactatat
     7801 tcccagctat tgtcggagat ttctttggat taaaaaacca tagtacaaat tacgggattg
     7861 tctaccaagg ttttggattt ggtgcgcttg caggatcatt tattggagcg atactcgggg
     7921 gatttcaacc aactttcatt ataatcggtg ttttaagtgt tatttccttc attatttcaa
     7981 tattaatccg tccaccaaat gtagaaaaga aaaaggaact aaaacattta catcggaaag
     8041 tagcttaatt ttaaaaagag tcctcatact agggctcttt tttattccat taactttgct
     8101 tctttactaa ctcattaaag acaggacaat atcattttaa aattgaatag ataagtaaaa
     8161 aggagcgtga cacttaaaat gaaaatagta cagcagtggg tacaagaaga tagtgattac
     8221 ataagagaga aagtaattga atacaatcaa aaatatcttg caggtgaaga aaaaacacct
     8281 tcagaaaagg ttagtttcat agtaagaaat gaaaaagaag aaatagtagg tggggtaaca
     8341 gcaataactt tttggcacca tatacacgtt gactttctat gggtttctga agaatatagg
     8401 catgaaggtt acggaagcaa gttaataaag cttattgaag agtttgcaat tgaaaaagag
     8461 tgcaggttaa tcaatttaga tacttttagt tttcaggcac ctgattttta taagaagcat
     8521 ggatataaag taatcggagt aagtgaagat catccgaaag gacataatca ttattattta
     8581 gaaaaacggc taaaaagtat atagaatttg ttaacattag tatttggaat aaacatgcaa
     8641 taagggcgag gttattatta atttcagtat taattgaatc aatttttatt ctaaaactgc
     8701 aagaacgtac tttggataat cttcctaagg tacgttcttt ttttatatca gtagcctatt
     8761 cttattaatt ccaccaatac tggtaaaggc aagacttaac taacttatat atattaaaag
     8821 gagttaatgg taaaatgaat agaagacaga aaattccttt tattaaaaat agaggaggta
     8881 catatgaaaa aagtaaatgt tacatatgca cttttatacg ataaaacaaa cgaaaagatc
     8941 ttaatggtaa aaaacaaagg ggaaaatggt tcttattata cattaccagg tggcgcagtt
     9001 aaattaggag aaacgttaga agaagcagta attcgagaag taaaagaaga aacagggtta
     9061 catataaatg taaacgggat ttgttctatc agcgaagcct tttttgagga aagagatcat
     9121 catgcaattt tctttaattt tctaggtgaa ataattggcg gagagacata tatatcaagg
     9181 ccaaaagaaa ttgaagagat tacttggatg gaattacata tagcagcacc gtatttacgc
     9241 ataccagaac atttactaga tttattacaa aagaaagaaa cagtacctta tttttttaac
     9301 ggaacaatcg ttcatcaatc ttcataaaaa tattatctca tgtaaggcgg ggaaaacatg
     9361 gagtttatcg ttaatcacaa acaattcact caagcacttt cagaagtaag taaagcaata
     9421 tcaacaaaat ctttaattcc tatattatct ggtataaaaa taactgcaga tcaatctgga
     9481 attactttaa tcgcaagtaa ctcgaatatt tttatcgaaa aattcattcc tgtttcaatt
     9541 gaagacgaaa agattgcaac tattttaaag gcaggaacta ttgttgtacc tgcaaagtat
     9601 ttcattgaaa taataaagaa aatgccaagt gatatagcaa taaaaagtaa gaatgagcaa
     9661 ttaattacga tacaatcaga agaaatcaca ttaagtttaa atggatttcc tgcagatgag
     9721 tttccgaatg taccgcttat agacaatcat gcagaaatca aagtagagac agagcaattg
     9781 attgaagtat ttaaacaaac agtctttgcg gtagcgaaaa atgaatctag acctgttctt
     9841 actggggtgc atatagagtt atctaataat aagttaattt gtgctgcaac tgactcacat
     9901 agactagcga tacgtgaaac attgctttcc tcagatgtaa aagcaaattg tattgtacca
     9961 agcgcaacca ttagtgaact tctaaaatta atgaacagca attcagaatt cgtatatatc
    10021 tatctttcag agagtcatat tatatttacg ctcggaacaa ctacattata ttcaagacta
    10081 atcgaaggta aatatcctaa tatttctaat cttattccga acgactttaa aacgattatt
    10141 aacatagata gaaaaaagat attacaaggt gtagaccgat caagtttatt agcaagtgaa
    10201 tgggccaata acaatgttaa cttagaaatc ataaacgaat ctacaattaa aatttcttca
    10261 aacgctt
//
