LOCUS       NZ_MOOL01000009        13320 bp    DNA     linear   CON 03-APR-2024
DEFINITION  Bacillus thuringiensis serovar xiaguangiensis strain BGSC 4BN1
            contig000009, whole genome shotgun sequence.
ACCESSION   NZ_MOOL01000009
VERSION     NZ_MOOL01000009.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis serovar xiaguangiensis
  ORGANISM  Bacillus thuringiensis serovar xiaguangiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Comparative genomics of Bacillus thuringiensis reveals a path to
            pathogens against multiple invertebrate hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13320)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, 1st Shizishan,
            Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.5.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002147905.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 02:08:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,596
            CDSs (total)                      :: 6,434
            Genes (coding)                    :: 6,198
            CDSs (with protein)               :: 6,198
            Genes (RNA)                       :: 162
            rRNAs                             :: 15, 15, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 1 (5S, 23S)
            partial rRNAs                     :: 15, 12 (16S, 23S)
            tRNAs                             :: 114
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 95 of 236
            Pseudo Genes (incomplete)         :: 153 of 236
            Pseudo Genes (internal stop)      :: 70 of 236
            Pseudo Genes (multiple problems)  :: 63 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:39:27
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 13320
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOOL01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..204)
                     /locus_tag="BK733_RS00535"
     CDS             complement(<1..204)
                     /GO_component="GO:0043592 - exosporium [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR03720.3"
                     /locus_tag="BK733_RS00535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="exosporium leader peptide-containing protein"
                     /protein_id="WP_140158793.1"
                     /transl_table=11
                     /translation="MLKNEKSQFDNINHISASLLNPNLVGPTFPPIPAFTLPTGPTGPT
                     GLTGPTGLTGPTGLTGPTGLTGP"
     source          1..13320
                     /country="China"
                     /db_xref="taxon:180870"
                     /isolation_source="bean"
                     /mol_type="genomic DNA"
                     /organism="Bacillus thuringiensis serovar xiaguangiensis"
                     /serotype="H51"
                     /serovar="xiaguangiensis"
                     /strain="BGSC 4BN1"
                     /submitter_seqid="contig000009"
     region          1..13320
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="CDPS"
                     /region_number="1"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..13320
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13320
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[4755:5475](-)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(4756..5475)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            <300..611
                     /locus_tag="BK733_RS00540"
                     /old_locus_tag="BK733_00475"
                     /pseudo=""
     CDS             <300..611
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189823.1"
                     /locus_tag="BK733_RS00540"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_00475"
                     /product="excisionase"
                     /pseudo=""
                     /transl_table=11
                     /translation="KLGVTNMRLSISSDTIRLEIKPFVLQVDPLQFQEEIRYLHSHMKS
                     GTILPHVEGIYFKSNVEPLTFHADHEFKQKIVQMAADAGMGQEEFLLQAVKSYMANLE"
     gene            complement(865..2079)
                     /locus_tag="BK733_RS00545"
                     /old_locus_tag="BK733_00480"
     CDS             complement(865..2079)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082482.1"
                     /locus_tag="BK733_RS00545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_00480"
                     /product="MFS transporter"
                     /protein_id="WP_088019791.1"
                     /transl_table=11
                     /translation="MNRVKSSASFLLYLLCISAFFASLNQNIYSPVIPIIRDSFQVSIT
                     MVNLSVSIFIFITAIMQIVFGSIIDFKGARTILIPSIILTIVASIGCAITSNFTVFLIC
                     RILQAVGTAAIPLIAATTIGSVFQGEQRGSAMGTYQTMLSIAPAVAPVLGGFIGEKYNY
                     PGVFWLLVAVSLILFVTNWIYFPKDRVGSKKELSGKTLISHYASIFKSKVGSSILILSF
                     FAFFLYFSFIVYLPILLTDHYQLSLQVVGLLYLPMAISLMIGSMTFKTIQSRISQKKLF
                     ITGNVVLALSVILFAVTHSKTLIGVTVALILHGISIGILTPLFATMIANEFEHNRGSAM
                     GMFNFIRYMGMAVGPIVSGVLLAHFKPTFVFALFGGFFIFLSFGMLIRIRRRHIKIVPK
                     EETLK"
     gene            complement(2137..2613)
                     /locus_tag="BK733_RS00550"
                     /old_locus_tag="BK733_00485"
     CDS             complement(2137..2613)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032352.1"
                     /locus_tag="BK733_RS00550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_00485"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_044585258.1"
                     /transl_table=11
                     /translation="MNKGIKQKIYDNYLHLLHLNEQKADKDIETFFKQAKMEEIEHVPT
                     NMTSIHVIACIGENQPINNITIARKMNLSKANITKINTKLIKENLITRFQSVDNKKEVY
                     YQLTPAGQSLCELHQKIHDQKENEFYQFVDSFSEIEQNAILKFLQNINDKMLGK"
     gene            2906..3376
                     /locus_tag="BK733_RS00555"
                     /old_locus_tag="BK733_00490"
     CDS             2906..3376
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017658172.1"
                     /locus_tag="BK733_RS00555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_00490"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_048530229.1"
                     /transl_table=11
                     /translation="MTNFVHEQTLKAIEDFVITREKKKYASQEMIKELVSEYENINKNW
                     TLTQLHIVSVIHESKQQMNNTLLSMELNISKATISKAIRVLIDNKILLTHQNTDNKKEI
                     FYTLTDKGIQLAIVHKKLHKIAHERYSELFQQFNDSELQVVTKFLEAWKQCI"
     gene            complement(3500..4720)
                     /locus_tag="BK733_RS00560"
                     /old_locus_tag="BK733_00495"
     CDS             complement(3500..4720)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017658173.1"
                     /locus_tag="BK733_RS00560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_00495"
                     /product="cytochrome P450, cyclodipeptide
                     synthase-associated"
                     /protein_id="WP_088019793.1"
                     /sec_met_domain="p450 (E-value: 1.1e-38, bitscore: 123.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNTIQTVNILTEEFQKNPYKYFSYLRQNDPVHYEGEIDSYFISR
                     YQDVRNILNDTETFTTKSLAERAEPVMRGPVLAQMRGKEHVAKRKIVLRSFMGDALQKL
                     MPLIKKNAEDLLFPHLPNGKIDLINDFGRTFAVYVTMDMIGLDKKDHKKIGEWHSGVAD
                     FITSINQPPEAKKHSLWCSEQLANYLEPIIKERRLNPQEDLISKLCSAKYEGIAMTDTD
                     ILALILNILLAATEPGDKTLALLIYNLINQPKQLQDVLSDRSLVPLAIAETLRYNPPVQ
                     LIPRQLSKDTDISGIQLSKGTTVFCMIGAANRDPNAFERPDEFNIYRPDLDIKKAFSGA
                     ARHLAFGSGIHNCVGAAFAKSEIEIVVNVVLDNMKNIKLEEDFQYVEKGLYTRGPISMP
                     ILFDKLV"
     gene            complement(4756..5475)
                     /locus_tag="BK733_RS00565"
                     /old_locus_tag="BK733_00500"
     CDS             complement(4756..5475)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180150.1"
                     /locus_tag="BK733_RS00565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_00500"
                     /product="tRNA-dependent cyclodipeptide synthase"
                     /protein_id="WP_069604038.1"
                     /sec_met_domain="CDPS (E-value: 2.3e-74, bitscore: 239.6,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNAIAVRNVRKFSSQPLSTNCAEILKRSKHAIIGISPFNSRFSD
                     EYINRLIEWALHTFDDVSVLLAGKEAANLLEALGTPKGKAERKVRKEVSRNRRSAEKAL
                     KEHGGNVNAIHTFSDFNDNNAYSCMRAEAEHIFLSETDFRNACLEMSHAAILGRARGTN
                     IDIDQISNDMLNIAVEYVIAELPFFIGGAEILGAQEAVLIYHKPWELGEQIVRNDFSIK
                     MKPNQGYLMVQELEDLS"
     misc_feature    5659..5672
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 22.49"
                     /tool="antismash"
     gene            5965..7620
                     /locus_tag="BK733_RS00570"
                     /old_locus_tag="BK733_00505"
     CDS             5965..7620
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750713.1"
                     /locus_tag="BK733_RS00570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_00505"
                     /product="M4 family metallopeptidase"
                     /protein_id="WP_088019795.1"
                     /transl_table=11
                     /translation="MKKTFAALVVSGITLSTFNLNISAEEDIQKQEVLKKMEIKQENWS
                     DNQENISFLSGKLSDKKVENQKQIEDFLKENVELFKMDARTDLKLTDIKSDTLGMKHYN
                     YVQMFKNVPIEGAQFIIHINKEGNVTAVNGDLHSNIKTNMPENTSKITKDKALSSAWEH
                     ININPNDTLVETKDNKVQGQEKLNSPIEKSEILIYKKDKNYYLAYKVELQFIKPYGANW
                     KIYVNALDGTIIEATNLVEDAIIPHVGYGNGVLGDRKPLNTTYIESYGNYALLDITKPM
                     NGGQIETYTALNAPSDNFINYSLHSQNNSWLDQKHAAAVDAHYNTGKVYDYYKNVHGRN
                     SFDGYGATIRSTVNAGYNESNAYWNGSQMVYGDGDNYRFRPFSAALDVVAHELTHAVTQ
                     YTAGLLYYKQSGALNESFSDVFGYFLDPEDWDCGEDLFTAEFSGHALRSLSHPEKYGQP
                     SHMNQYRYMQDDNGGVHVNSGIPNKAAYLTINAIGKEKAELIYYRALTMYLTPTSNFRD
                     ARAALSQSALDFDCYYGWHTYRAVENAWNQVGVF"
     misc_feature    complement(7684..7697)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.67"
                     /tool="antismash"
     misc_feature    7685..7698
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.94"
                     /tool="antismash"
     gene            7719..>8012
                     /locus_tag="BK733_RS33995"
                     /pseudo=""
     CDS             7719..>8012
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611143.1"
                     /locus_tag="BK733_RS33995"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAKFSSTQKIQAVKRYLNGTES"
     misc_feature    complement(7720..7736)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.81"
                     /tool="antismash"
     gene            8072..8284
                     /locus_tag="BK733_RS00575"
     CDS             8072..8284
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024997.4"
                     /locus_tag="BK733_RS00575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="WP_254907470.1"
                     /transl_table=11
                     /translation="MYETNIKVTVQLIYWYRVIDLKGNIIEFLLRKTRNLQTANCFFNK
                     YLRFFHVSNPRAITGDKKLANPLYA"
     gene            8903..9427
                     /locus_tag="BK733_RS00580"
                     /old_locus_tag="BK733_00510"
     CDS             8903..9427
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003266.1"
                     /locus_tag="BK733_RS00580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_00510"
                     /product="ABC transporter permease"
                     /protein_id="WP_088019797.1"
                     /transl_table=11
                     /translation="MDEKPLRNEQPAQTSTSSCSTEEERQIPFCCVISVPHGFQPVPYC
                     KSRLVYNLSCLGTVKETCRKIIQVDDCGQTEIDLHILKIKGCISFLVNTEVEPTCERDV
                     CSSIPHTKEIILSCEGNVCVDHIIKCSVDCLPDVCLDCENILVRDLQVQPICEDECHFV
                     KVTGYFQLDVI"
     gene            complement(<9425..9721)
                     /locus_tag="BK733_RS00585"
                     /pseudo=""
     CDS             complement(<9425..9721)
                     /GO_function="GO:0000150 - DNA strand exchange activity
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204471.1"
                     /locus_tag="BK733_RS00585"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="recombinase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLQNYNCTDILTEKMTGTKSDRPGLNRLKDKIRFSDGKFFRIRTK
                     YKRFN"
     misc_feature    9713..9726
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.93"
                     /tool="antismash"
     misc_feature    10007..10021
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 24.8"
                     /tool="antismash"
     gene            10132..11501
                     /locus_tag="BK733_RS00590"
     CDS             join(10132..10597,10597..11501)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1026: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611142.1"
                     /locus_tag="BK733_RS00590"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="IS3-like element ISBth167 family transposase"
                     /protein_id="WP_088019799.1"
                     /ribosomal_slippage=""
                     /transl_table=11
                     /translation="MAKFSSKDKIQAVKRYLEGTEGGKTIANSIGVHPRELYQWIKRFE
                     FSGEKAFEKRYTTYSLEYKLDVIHYMNENGKSLRETAAFFNIPSCETLRKWKVAYGTEG
                     LDALKSKKKGRLTMAKEKAKLQHLKQNEVSLEGSIEALQAENERLRMENDYFKKVECLS
                     SKEENITDQDKAQLIYELRHKYKVVDLVKVANIARSTYYYWMKQAKRPDKYKKVKELIK
                     EFFSENFGRYGYRRITLELRNRGHALNHKTVRRLMNILGLKCLVQLKKYRSYKGTVGKF
                     VPNILKRNFHASKPNEKWVTDVTEFHLHGKKLYLSPILDLYNGEIIAYNIEHRPAYSLV
                     SKMLNKAFQRLNDKETPILHSDQGWHYQMRQYHQSLKKHNVIQSMSRKGNCLDNAVMEN
                     FFGLLKSELLYLKEFESMEQFKQELETYIHYYNHKRIKTKLKGLSPVQYRVQSLVAA"
     misc_feature    complement(10133..10149)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.81"
                     /tool="antismash"
     gene            complement(11708..12205)
                     /locus_tag="BK733_RS00595"
                     /old_locus_tag="BK733_00525"
     CDS             complement(11708..12205)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933897.1"
                     /locus_tag="BK733_RS00595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_00525"
                     /product="hypothetical protein"
                     /protein_id="WP_088019800.1"
                     /transl_table=11
                     /translation="MLNNWLTLSKIEIETEIPYVILRRYIRGHGHHLKMKKQKSGYLIA
                     KEFISKLLKINLYYQEGKSFEQIEEILCLHCKFREYSLEENEESITNQIMNALIHMNEK
                     LFDLNQKYEDLVKEFQMQKENLNNKSLENSSLVLQRDEQDKVYQKSRIILGNGKRWSFF
                     KY"
     misc_feature    12602..12617
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.54"
                     /tool="antismash"
ORIGIN
        1 cggtccagtc aatccagtcg gtccagtcaa tccagtcggt ccagtcaatc cagtcggtcc
       61 agtcaatcca gtcggtccag tcggtccagt cggtaatgta aaagcaggta tcggtggaaa
      121 tgttggtcct acaaggttgg ggtttaataa cgatgcagat atatgattta tattatcaaa
      181 ttgacttttt tcattcttta gcataaattc acctccgttc cttatttttt atgcaataaa
      241 atttaaaaaa tgcatttaag atcaaagtaa tacttatcca atatattatg atattactta
      301 aattaggtgt cacaaatatg cgattgagta tttcatcaga tactatcaga ttagaaatta
      361 aacctttcgt attacaagta gatccattgc agtttcaaga ggaaattagg tacttacatt
      421 ctcatatgaa atctggcacg atccttccac atgtagaagg tatttacttt aaaagtaatg
      481 tagagccatt aacttttcat gcagatcatg aattcaagca aaaaatagtg caaatggcag
      541 cggatgctgg tatgggacag gaagaattct tattgcaagc tgtaaagtct tatatggcaa
      601 atttagagta gcaataaatt tattatcaaa tagaaaaagt gtagaaataa aatctttcta
      661 cacttttttt tactttactt aattttattt ctcttgttaa catttctaat tacttataca
      721 tacaataaca atgggataga gttttaactt actttattcc aaaaatacta gatgcaatag
      781 attaagccct ttattatgaa gaataatttc cttatatatt ttatacacta tgaattttaa
      841 attataggat cataaatttt tttcttattt caaagtttct tctttaggta caattttaat
      901 atgtctcctc ctaatccgaa taagcatgcc aaaagagaga aagataaaaa acccaccaaa
      961 tagagcaaat acaaatgtag gcttgaaatg tgctaataat acccccgaaa caataggtcc
     1021 tacagccatt cccatatatc taataaagtt aaacattccc attgcacttc ctctattatg
     1081 ttcaaattca tttgcaatca tagtagcaaa taaaggagta aggataccaa ttgatattcc
     1141 gtgaagaatt aaagcaactg ttacgccaat taaagttttc gagtgtgtca cagcaaataa
     1201 tataacgctc aaagctaaga ctacatttcc agttataaaa agcttttttt gtgaaattct
     1261 tgattgaatt gttttaaaag tcatgctgcc aatcattaaa ctaatagcca ttggtaagta
     1321 aagtaaaccc actacctgta aacttaattg ataatgatca gttaataaaa ttggtaagta
     1381 aacaatgaag gagaaataaa gaaaaaatgc aaagaaactt aatattaata tggaactgcc
     1441 cactttgctt ttaaatatag aagcatagtg agatataagt gttttcccac tcaattcttt
     1501 tttacttcct acacgatcct tagggaaata tatccaatta gttacaaata gaattaaaga
     1561 gactgccaca agtagccaga aaacacctgg gtaattatat ttttccccaa taaaacctcc
     1621 tagcacaggt gcaacagcag gtgcaatgga taacatggtt tgatatgtac ccattgcact
     1681 tcctcgttgt tctccttgaa atacactacc aattgtagtt gcagcaataa gtggtattgc
     1741 agctgtccct acagcctgta aaattctaca tattaaaaat actgtaaagt tacttgtaat
     1801 tgcacaacca atactcgcca caattgttaa aataatactc ggaataagaa tagtcctagc
     1861 acctttaaaa tcaattatag aaccaaaaac aatttgcata attgccgtaa taaaaataaa
     1921 tatggaaacg gaaaggttaa ccattgttat agatacttgg aatgaatccc gaattatggg
     1981 aattactgga gaatatatgt tttgatttaa agaagcgaaa aaagcactaa tacacaacaa
     2041 atataaaaga aaagaagcac ttgatttaac acggttcatg ctatccctcc taaaagatta
     2101 ttaataatta gctaatttat ttaagttata ataaatttat ttccccaaca ttttatcgtt
     2161 tatattttgt aaaaacttca atattgcgtt ttgttcaatt tcagaaaatg aatcaacgaa
     2221 ctgataaaat tcattttctt tctggtcatg aattttctga tgtaattcac aaagtgattg
     2281 tcctgccgga gtaagttgat aatatacctc ctttttgtta tcaactgact gaaaacgagt
     2341 gattaaattt tcttttatta atttggtgtt aatttttgtg atattcgctt tagataaatt
     2401 cattttcctt gcaattgtaa tattatttat tggttgattc tcaccaatac aggcaattac
     2461 gtgtatactt gtcatatttg tcggtacatg ttctatttct tccatttttg cttgcttaaa
     2521 aaaagtctca atatccttat ctgccttttg ttcattgaga tgtaacaaat gtagataatt
     2581 atcgtaaatt ttctgtttta tgcccttatt catttgaatg tttctacccc tctttgtcta
     2641 ttttgttttt ttgtttaccg gtaaacaatt ttgattttat atcctcattt agcaattgtc
     2701 aattgctaaa ttggaattta actaattatg taagaacctt taatgtatct gtttttcata
     2761 atttacccta ataaagccct attatattaa agagttagtt tcaatgcata aaagtctttt
     2821 tttaaattgt tgacctaatt attgtttact gataaacttt atattgttaa ccggtaaaca
     2881 ataattctag ataggggaaa ttattatgac aaattttgtc catgaacaaa ccttaaaagc
     2941 tattgaagac tttgtcataa caagagaaaa gaaaaaatat gcatctcaag agatgataaa
     3001 agaattagtt agtgaatatg aaaatataaa taaaaattgg actctaacgc aactacatat
     3061 tgtttcggtt attcatgaga gtaaacaaca aatgaataac actctactat ctatggaact
     3121 taatatttct aaagcaacca tctctaaagc tattcgcgtt cttattgata ataaaatttt
     3181 attgacgcat caaaatacag ataataagaa ggaaatcttt tatactttaa ctgataaggg
     3241 aatacagtta gccattgttc ataaaaagtt acacaaaatt gcacatgagc gttacagtga
     3301 gttattccaa caatttaatg attccgagtt acaagttgta acaaaatttt tagaagcatg
     3361 gaaacaatgt atttaatttt ttgttatatc agcttagaag gtatatatgt tgaccttctc
     3421 ataaaaatag aaaaactaag gggttttagc cccttagtaa aaattgaatt atattaataa
     3481 ctaaaatagc catgtttttt taaactaact tatcaaaaag aataggcatg gaaataggtc
     3541 cacgtgtata aagtcctttt tcaacatatt gaaaatcttc ttctaattta atatttttca
     3601 tattatctaa gactacattt acaactattt caatttctga ttttgcaaac gctgctccta
     3661 cgcaattatg aatacctgat ccgaatgcaa gatgtcttgc agcaccacta aatgcctttt
     3721 taatatcaag atccggacga taaatattga attcatcagg tctttcaaat gcattagggt
     3781 cacgattcgc tgcaccaatc atacaaaata ccgtagttcc tttggatagt tggataccac
     3841 ttatgtccgt atcttttgac aattgacgtg ggatcaattg cactggtgga ttgtaacgta
     3901 aagtttctgc aatagctaat ggtactaatg aacgatcact taatacatct tgcagttgct
     3961 tcggttggtt aattaaatta tagattaata aagcaagagt cttatcacca ggttctgttg
     4021 cagctaataa tatatttaaa atcaatgcta gaatatcagt atcagtcata gctattcctt
     4081 catattttgc actgcataat ttagaaatta agtcttcctg tgggtttaat cttcgctctt
     4141 taataattgg ttctaaataa ttagctaact gttcactgca ccaaagagaa tgtttttttg
     4201 cctctggtgg ttgatttata ctggtaataa agtcagctac accgctatgc cattctccta
     4261 tcttcttatg atctttctta tctaatccaa tcatatccat tgttacataa acagcaaatg
     4321 ttctgccaaa atcattaatt aaatctatct ttccatttgg aaggtgtgga aatagtaaat
     4381 cttctgcatt ctttttaatt aaaggcatta atttttgtaa agcatctccc ataaaacttc
     4441 ttaaaacaat ttttcttttc gcaacatgct cttttccacg catttgtgct aatactggtc
     4501 cacgcattac tggctcagca cgttcagcta atgattttgt tgtaaatgtt tcagtatcat
     4561 tcaatatatt acggacatct tgatatctgc taataaaata actatcaatc tcaccttcgt
     4621 aatgtactgg gtcattctga cgtaggtatg aaaaatactt atatggattt ttttgaaatt
     4681 cctctgttaa tatatttacc gtttgtatag tatttgacat atttcttccc ccttgtattg
     4741 gatacttact atttgctaag ataaatcttc caattcttga accattaaat atccttgatt
     4801 tggtttcatc ttgatagaaa aatcatttcg aactatctgt tcaccaagct cccatggttt
     4861 gtgataaata agtacagctt cttgagctcc taaaatttca gctccgccaa tgaaaaatgg
     4921 gagttctgca attacatatt ctactgcaat atttagcatg tcatttgata tttgatcaat
     4981 atctatatta gtaccccttg ccctacctaa aatggctgca tgtgacattt ctaagcaagc
     5041 atttcgaaaa tcagtttcgc ttaaaaaaat atgttctgct tctgccctca tgcagctata
     5101 tgcattgttg tcattaaaat cagaaaaagt atggatagca tttacattac caccatgctc
     5161 tttaagtgcc ttttcagctg atcttctatt tcgagatact tctttcctaa cttttctttc
     5221 cgctttacct tttggtgttc ctagagcctc aagtaaattt gcagcttctt ttccagctaa
     5281 taaaacacta acatcatcaa aagtatgtaa tgcccattca atgagtctat taatatattc
     5341 atcagaaaat ctactattaa acggactaat acctattatt gcatgcttac tacgttttaa
     5401 tatttcagca caattagtag ataagggttg agaactaaac tttcgtacat ttcttaccgc
     5461 tatagcattc gtcatacaat catcccttta aataatttta tcaatataaa ccgttttatt
     5521 gattttaaat agccaataat ctttcaaact acataaaaaa gagatagaat atcttgatgt
     5581 tagaaataat gaataaaaat aatactaaac aaaaaccctg cataatgttc accggtgaac
     5641 aaaaccaatt ctatattgca aatgtttgtt tgtcaatata gattattctg aaaaatagat
     5701 aaaacaataa agtgctacgt gagcagttaa aatttgttca tacatagatt tataaaaaaa
     5761 tctaaaagga ctccgttcct tacttaacaa aagagactag ttgaagccag ccaccaaaaa
     5821 cttaagatag agattaaaac gttggttatt taagctgcta agagcgtatc aacactcttt
     5881 atgcaatatt acatcttttt tatactaaaa atttatatat actattttaa ggattttaaa
     5941 tctatttaga aaggtgttgt ttgtatgaag aaaacttttg ctgcgcttgt agtatctgga
     6001 attacattaa gtaccttcaa tcttaatata agcgctgaag aggatataca aaaacaagaa
     6061 gtattaaaga aaatggagat taaacaagag aattggagcg ataatcaaga gaatatatct
     6121 ttcctttcag gtaaattatc cgataaaaaa gtggagaatc aaaaacaaat tgaagatttt
     6181 ttaaaagaaa atgtggaact atttaaaatg gacgcaagaa cagatttaaa attaacagat
     6241 ataaaatcag atactctggg aatgaaacac tataactatg tacaaatgtt caagaatgta
     6301 cctatagaag gtgcacaatt tattattcat ataaataaag aaggaaatgt aactgctgtc
     6361 aacggggact tacattcaaa tataaaaaca aatatgccag agaacacatc caaaattaca
     6421 aaagataagg ctctatcttc tgcttgggag catataaata ttaacccaaa tgataccctg
     6481 gtagaaacaa aagataataa agttcaggga caggaaaaat tgaattcccc tatcgaaaaa
     6541 tcagaaattc ttatctataa aaaagataaa aattattatt tagcttataa ggtagagttg
     6601 caatttataa aaccatacgg cgctaattgg aaaatatatg tgaatgcact tgatggcaca
     6661 attattgagg caactaattt agtagaagat gctatcattc cacatgttgg ttacgggaat
     6721 ggagtgttag gtgatcgtaa acctttaaat acaacatata ttgaatcata tggaaattat
     6781 gctttattag atattacaaa acctatgaac ggtggacaaa ttgaaactta tactgcactt
     6841 aatgctccgt ctgataattt tataaattat agtttgcata gccaaaataa ttcatggttg
     6901 gatcaaaaac atgctgccgc agttgatgct cactataata caggaaaagt ttatgattat
     6961 tataaaaatg tacatggacg caatagtttt gacggctatg gtgcaaccat tcgttctact
     7021 gtaaatgctg ggtataatga gagcaatgcc tattggaatg ggagccaaat ggtgtatggg
     7081 gatggagata actatcgttt tcgccctttt tctgccgcac ttgatgtggt cgctcatgaa
     7141 ctaacacacg ctgttacgca atacactgca ggtctattat attataaaca atctggtgca
     7201 ttgaatgagt ctttttctga tgtttttgga tactttctag atccagaaga ttgggattgt
     7261 ggagaggatt tatttacagc tgagttctca ggtcatgcat tacgcagctt atctcatcct
     7321 gagaagtatg gtcaaccttc tcatatgaac caataccgat acatgcaaga tgataacggt
     7381 ggtgttcatg ttaatagtgg tatcccaaat aaagcagcct atttaacaat caatgccatt
     7441 ggaaaagaaa aagcggaact tatatactat cgagcattaa caatgtattt aacacccaca
     7501 agtaatttta gagatgctcg tgcagcatta tcacaatcag ctttagattt tgattgttac
     7561 tatggttggc atacataccg tgctgttgaa aatgcctgga atcaagttgg tgtattttag
     7621 cttgaaatta aatattcata tatttaaatt gatttgaatg caaaatgaag tgcacaccaa
     7681 ttgttagaca cagtctaaca attggtggtg cattttttat ggctaaattt tcttcaacac
     7741 agaaaatcca agcagttaaa cgatatttaa acggtacaga aagctgaaaa acaattgcta
     7801 aatctataga agttactcct agcttacttc gtgagtggat tagacgatat gaatcttcag
     7861 gtgaaaatgc ttttgaaaaa cgctatatat cctactctac ccagtataaa ctagacgtac
     7921 ttaattatat gaatgaacat gggacatcta tcagagaaac agcggcgctt ttcaatattc
     7981 cgtcttatga aacacttccg aaatggaaaa tggttatgcg ttggattcat caataggatc
     8041 ctgaatgaga taaacaaata tctcttggag aatctatgaa acaaatatca aggtaacagt
     8101 tcaattaata tactggtatc gtgttattga tttaaaagga aatataatcg aatttctcct
     8161 aagaaaaaca agaaatcttc agactgcaaa ttgctttttc aataaatatt tgcggttttt
     8221 tcatgtttca aatccccgtg ctataacagg cgataaaaag ctagctaatc cgctctatgc
     8281 ttgaattcaa atactttgac tcagctgcat tctttttaga gaagaagcca cgcatatgat
     8341 aaaaacggtt aattttcaaa atcaatctgc ccaaaatcag aaagaattca tccatcaatt
     8401 gcttgtacct gcagcataag atatgactcc gttaggaata tacgtactat attttttatt
     8461 tttgcaccag aatctgttaa caagaatgtc tatgcacaat tacactatac aatttctctt
     8521 agttaaaatt aatgaatgct tttttataaa aaagaataaa ttcgcaccaa tagatacttc
     8581 ttttcataga ttatgagagg aggtgtatat caagaataag ggggttgtaa aattcgtatt
     8641 taacaataaa aaagaatggt ggaatcctat ttcgctgcat tatcgttata acaaagaatt
     8701 tttaatgcgt ttctgactaa aaaactcaaa caatatttta gataagttat ttagaaatca
     8761 acattacttt ttgctcagga atccatgaaa gtgcatagta actccgccca acaataaata
     8821 ggaatcttcc atttttttat gaaaataaaa cgtcttacca aagaataatt caaattgtat
     8881 taaagaaagg tgataaatat ttatggatga gaaaccatta cgcaatgaac aacccgcgca
     8941 aacgagtacc tctagttgtt caacagaaga ggaacgtcaa ataccattct gttgtgtcat
     9001 ttctgtgcca cacggttttc aaccagttcc atattgtaaa tctagattag tctataacct
     9061 aagttgtctt ggtaccgtaa aggagacatg tagaaaaata atacaagtcg atgattgtgg
     9121 acagacagag attgatttgc atattttgaa gattaaaggc tgtatttcat ttctagttaa
     9181 tactgaagtt gaacctactt gcgaaagaga tgtttgttca agtattccac atacaaaaga
     9241 aataattctg agctgtgaag gaaatgtatg tgtagatcat ataataaaat gtagtgtaga
     9301 ttgtttaccg gatgtttgtt tggattgtga aaatattcta gtaagggatt tacaagtaca
     9361 acccatctgt gaagacgaat gtcatttcgt aaaagttaca ggatattttc aactagatgt
     9421 tatttaatgc tctttcaata aagctattct taacacaagg gcgtctccat actctccctc
     9481 ttaagtacgt tgtgcaatta aatctctttc aaactgttct ttaatactaa ttagttttac
     9541 acccttattt tgaaaatact caactagttc aattaaatct tttgtacttc gtcctaatcc
     9601 ggaaaaactt tccatcacta aatctaattt tatcttttaa tcggttaagc cctggtcgat
     9661 cactttttgt tcctgtcatt ttttctgtca aaatgtcggt acagttataa ttttgaagca
     9721 tatcaatttg tcgaattaaa tcttgctact ctgtatttac cctggcataa ccataaatat
     9781 atgaactcat aaaaccacct accctttaca caatattttt atcataaagg gtaggtaaat
     9841 tttaatttat ttttgacaat agttttgaca acgaagttaa gcgatttagc atgatcaaat
     9901 ttctcttaca aatgaaaaaa tataaacatt tcgtattggt ttaacaaccg ttgacattat
     9961 atcaggatcg ccattttcag gaaatattcg gaaatataat gctttcattt ttcacaaaat
    10021 tggatatcga gcatatagtc ttttttatcg attggagcta aaaaaagacc ctctagttga
    10081 agtgcccccc aattgttaga cacataatta acaattggag gtgtattttt tatggctaaa
    10141 ttttcttcaa aagataaaat acaagcagta aaacgatatc tagaaggtac ggaaggtgga
    10201 aaaaccattg ctaattctat aggggttcat cctagagaac tttatcaatg gattaaacgg
    10261 tttgaatttt caggggaaaa ggcgtttgaa aaacgctata caacttactc tctcgagtat
    10321 aaactagatg taattcatta tatgaatgaa aatgggaaat ctctgagaga aacagctgct
    10381 ttttttaata ttccttcttg cgaaacactc cgaaaatgga aagtagctta tggaacagaa
    10441 ggattagatg ccctaaaatc aaagaaaaag gggcgtctaa ccatggccaa agaaaaagct
    10501 aaactacaac atttaaaaca aaacgaagta tctcttgaag gttctataga agcgctacaa
    10561 gcagaaaatg aacgtctgcg tatggaaaat gactatttaa aaaagttgaa tgccttagtt
    10621 caaaagaaga aaacatcaca gaccaagata aagcgcaatt gatttatgaa ttaaggcata
    10681 aatacaaggt cgttgacctt gtgaaagtcg ctaatatcgc tcgtagcacg tattattact
    10741 ggatgaaaca agcgaaacgt ccagataaat ataagaaagt taaagaatta attaaagagt
    10801 tctttagtga gaattttggg aggtatggtt atcgtcgtat tacattggaa ttacgtaatc
    10861 gaggccatgc attaaatcat aaaaccgttc gacgtttaat gaatatcctg ggattaaaat
    10921 gtcttgttca attaaaaaaa tatcgttcat ataaagggac tgtcgggaaa ttcgttccta
    10981 atattttaaa acgtaatttt cacgcatcaa aaccaaatga aaaatgggta acagatgtga
    11041 cggagttcca cttacatggt aaaaaactat atctatcacc cattttagac ttatataacg
    11101 gagaaattat agcttacaat atagaacata gacctgccta ttctcttgtt tctaaaatgt
    11161 taaataaggc gtttcaacgt ttgaatgata aggaaactcc tattttacac tcggatcaag
    11221 gttggcatta tcaaatgcga caatatcatc agtcgcttaa aaaacataac gttatccaaa
    11281 gtatgtcccg taagggaaat tgcttagata atgcagtcat ggaaaatttc tttggcttat
    11341 taaaatctga attactttat cttaaagagt ttgaaagtat ggaacaattt aagcaagaac
    11401 tagaaactta tattcattac tacaatcaca aaagaattaa aacaaaacta aaaggattgt
    11461 cccctgtgca atacagagtt caatccttag tagctgctta atatatttgt ctaatttttt
    11521 gggttcagtt caagtttgat tttcatataa aaacgtattt aattttaaaa actacttttc
    11581 agaattaata actcaattta ttttttaatt attgtttgag ctcacggtat gcttttattc
    11641 gactattttt ccgttgaaat aaaaattcca caaatcaact tttattaatc gtcatatatt
    11701 tcaaatacta atacttaaaa aaactccatc tctttccatt acctaagatt atcctactct
    11761 tttgatacac cttatcttgt tcatctcttt gcaatacaag tgaactattc tctaatgatt
    11821 tgttatttaa attctccttt tgcatttgaa attctttaac taaatcctca tatttttgat
    11881 ttaaatcaaa taatttttca ttcatatgta ttaatgcatt cataatttga ttcgttattg
    11941 attcttcatt ttcttctaag ctatattcac gaaatttaca atgtaaacaa agtatctctt
    12001 ctatttgttc aaaactttta ccctcttgat aatacaaatt aatttttaat agttttgaaa
    12061 taaattcttt tgctattaaa tagccacttt tttgtttctt catctttaag tgatgtccat
    12121 ggcctcttat ataacgtcgt agaattacat acggaatttc agtttctatt tcaatcttgg
    12181 aaagagttaa ccagttattt aacatgcact atatgttccc ttctatatgg acttatttat
    12241 taaattataa aaaaatcaat catacataat agatatcttg tatacctatt ttaacttagc
    12301 tatttctctt caagtttttc acatactcga cttcttgtaa tgaattaaag ggcaaaaatc
    12361 cacctgtata gagcaaagct acgtgttcat catacattgg atgccatatg atgatatatt
    12421 attatccatt acagttaact ctatcaaatt gacataaagg gataccatat ttatcacttc
    12481 tatatacagt gattgcacca agcattaact tatcttaagg ttttccctta aaaatctcat
    12541 taaattcaat ttttcacata aatatttttg cttttttaac caataatcct cttattttat
    12601 ttcgacattt ttttcatttc tttaacaccc caatgtttca ttgcaataga tctacactag
    12661 ctaataaaat ttaagtttaa aatattcaat taatcgccct cctagtgaaa ggcccgctcc
    12721 gcatccagaa cgtactatct aatacctaaa agatttcatg cataaattct ctagaatatc
    12781 ccttaaaatc acataataaa aacataaatt aatatatctt ggaatcacac cctaacctgt
    12841 tattgaataa gcagtgtatc ttcctcaata tattttaaat cccaagaaaa aactcaactt
    12901 taatttttta ttgcatatat taaaagaggt agtagatata ctgatattga aattaactat
    12961 attaaagagg aaaaggagga gtaaatatag gtaattttcc acttgtaaaa acatttaaat
    13021 acttaattat gagtataagt aataaaacac ctattttgat cgttgcttta gacatttcaa
    13081 tttattgaac gcctcaaatc acttttaatg attacaatgg aaattaacaa acttatttat
    13141 gcatacttca aagttacaag ggctgcagga taaccagtct actgagtatg aagcatggca
    13201 cattcaccga aaaaataaat ctataggaaa aaacatactt tttccaaatg actttaagat
    13261 tattatctag aaataattag cggctcccat gtgtaaattt tttattttta gttaattatg
//
