LOCUS       NZ_MOOL01000003         3842 bp    DNA     linear   CON 03-APR-2024
DEFINITION  Bacillus thuringiensis serovar xiaguangiensis strain BGSC 4BN1
            contig000003, whole genome shotgun sequence.
ACCESSION   NZ_MOOL01000003
VERSION     NZ_MOOL01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis serovar xiaguangiensis
  ORGANISM  Bacillus thuringiensis serovar xiaguangiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Comparative genomics of Bacillus thuringiensis reveals a path to
            pathogens against multiple invertebrate hosts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Zheng,J., Gao,Q., Liu,H., Peng,D., Ruan,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, 1st Shizishan,
            Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.5.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002147905.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 02:08:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,596
            CDSs (total)                      :: 6,434
            Genes (coding)                    :: 6,198
            CDSs (with protein)               :: 6,198
            Genes (RNA)                       :: 162
            rRNAs                             :: 15, 15, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 1 (5S, 23S)
            partial rRNAs                     :: 15, 12 (16S, 23S)
            tRNAs                             :: 114
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 95 of 236
            Pseudo Genes (incomplete)         :: 153 of 236
            Pseudo Genes (internal stop)      :: 70 of 236
            Pseudo Genes (multiple problems)  :: 63 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:39:27
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 3842
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MOOL01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..3842
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:2531](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..2531)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     aSDomain        complement(<1..1010)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS00015_AMP-binding.1"
                     /evalue="8.90E-81"
                     /label="BK733_RS00015_AMP-binding.1"
                     /locus_tag="BK733_RS00015"
                     /protein_end="844"
                     /protein_start="507"
                     /score="263.5"
                     /specificity="substrate consensus: Asn"
                     /tool="antismash"
                     /translation="FEEQVKKVPNNIAVQYGDASLTYDELNKKSNQLAHYLKGQGVGPE
                     VMVGISMERSLDLIIGIFGVLKAGGAYVPIDPNAPSARIHYMLEDSMVPIVLSHQGLSN
                     RIYKDKVKVICLDMDWNEIEKMRTSDLIGEVQVNNLAYVIYTSGSTGKPKGVMVPHRGL
                     TNYLNWCTQNYYKGNGGQGSLVLSSISFDLTITGMLSPLCIGETVVLFPEGLDLEELGR
                     WVRKRKYSVIKLTPAHLELLSHQVSEEDAAFCTDTFVVGGEPLLAKHVDFWVKHAPNTV
                     IINEYGPTEAVVGCTNYIVKPGENYSKSIPIGKPIFNTEMYVLDTSLQPVPIGVHG"
     aSModule        complement(<1..2399)
                     /domains="nrpspksdomains_BK733_RS00015_Condensation_LCL.1"
                     /domains="nrpspksdomains_BK733_RS00015_AMP-binding.1"
                     /incomplete
                     /locus_tags="BK733_RS00015"
                     /tool="antismash"
                     /type="nrps"
     gene            complement(<1..2531)
                     /locus_tag="BK733_RS00015"
                     /old_locus_tag="BK733_00015"
     CDS             complement(<1..2531)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (44-342). E-value:
                     5.6e-110. Score: 359.0. Matches aSDomain:
                     nrpspksdomains_BK733_RS00015_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (507-844). E-value: 8.9e-81.
                     Score: 263.5. Matches aSDomain:
                     nrpspksdomains_BK733_RS00015_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406687.1"
                     /locus_tag="BK733_RS00015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_00015"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_140158790.1"
                     /sec_met_domain="Condensation (E-value: 3.9e-86, bitscore:
                     275.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.6e-78, bitscore:
                     251.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEREEDLLAKLESLSPEKKKFLMDRLKRNSPKNIMKKRRESDLPV
                     LSFAQQRLWFFDQLEPVSSTYNMPFLLNIVGDFDIDIFEKSLNEIIQRHEILRTTFLLM
                     DEQPIQIVNPNLSIKVKYVDLRAYSKEERQKMSDEQIKKFAKKPFNLEIGPLVRANIWQ
                     VEGKEYWMLLNMHHIVGDGWSVGILIQEISKVYNAFIKGDNSPLLPLTMQYADFSIWQK
                     GWLKGSKQQEQLNYWKKQLSGNLPVLDLPRDFSRPNKATYNGDEEYITIPKGLVEKLRT
                     LSQQEGGTLYMLLLAAYNILLYKYSKQEDIIVGTPIANRNHLEIEPLIGFFVNTLALRT
                     RLRKDMCFRELLQEVKQTCLGAYSNQDIPFENIVAEIVPERQSNSSVLFQTVFALEKQT
                     QNIIEMSGVKVRPEKLNINVAKFDLTLSMIEEEDKVSGTFNYNTGLFRKSTIQRISKHY
                     LSILEQVIENPNIKLNQLSLINKEELNQIIKRKHNVINNLQIDTTLPELFEEQVKKVPN
                     NIAVQYGDASLTYDELNKKSNQLAHYLKGQGVGPEVMVGISMERSLDLIIGIFGVLKAG
                     GAYVPIDPNAPSARIHYMLEDSMVPIVLSHQGLSNRIYKDKVKVICLDMDWNEIEKMRT
                     SDLIGEVQVNNLAYVIYTSGSTGKPKGVMVPHRGLTNYLNWCTQNYYKGNGGQGSLVLS
                     SISFDLTITGMLSPLCIGETVVLFPEGLDLEELGRWVRKRKYSVIKLTPAHLELLSHQV
                     SEEDAAFCTDTFVVGGEPLLAKHVDFWVKHAPNTVIINEYGPTEAVVGCTNYIVKPGEN
                     YSKSIPIGKPIFNTEMYVLDTSLQPVPIGVHG"
     source          1..3842
                     /country="China"
                     /db_xref="taxon:180870"
                     /isolation_source="bean"
                     /mol_type="genomic DNA"
                     /organism="Bacillus thuringiensis serovar xiaguangiensis"
                     /serotype="H51"
                     /serovar="xiaguangiensis"
                     /strain="BGSC 4BN1"
                     /submitter_seqid="contig000003"
     region          1..3842
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX) and not alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..3842
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /kind="neighbouring"
                     /product="NRPS"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    1..3842
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[2597:>3842](-)"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      complement(2598..>3842)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="2"
     CDS_motif       complement(531..590)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS00015_0008"
                     /evalue="1.60E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="BK733_RS00015"
                     /protein_end="667"
                     /protein_start="647"
                     /score="35.8"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGKPKGVMVPH"
     CDS_motif       complement(783..824)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS00015_0007"
                     /evalue="6.90E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="BK733_RS00015"
                     /protein_end="583"
                     /protein_start="569"
                     /score="19.1"
                     /tool="antismash"
                     /translation="FGVLKAGGAYVPID"
     CDS_motif       complement(1347..1466)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS00015_0006"
                     /evalue="1.90E-14"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="BK733_RS00015"
                     /protein_end="395"
                     /protein_start="355"
                     /score="45.9"
                     /tool="antismash"
                     /translation="KQTCLGAYSNQDIPFENIVAEIVPERQSNSSVLFQTVFAL"
     aSDomain        complement(1506..2399)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS00015_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="5.60E-110"
                     /label="BK733_RS00015_Condensation_LCL.1"
                     /locus_tag="BK733_RS00015"
                     /protein_end="342"
                     /protein_start="44"
                     /score="359.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVGDG"
                     /tool="antismash"
                     /translation="VLSFAQQRLWFFDQLEPVSSTYNMPFLLNIVGDFDIDIFEKSLNE
                     IIQRHEILRTTFLLMDEQPIQIVNPNLSIKVKYVDLRAYSKEERQKMSDEQIKKFAKKP
                     FNLEIGPLVRANIWQVEGKEYWMLLNMHHIVGDGWSVGILIQEISKVYNAFIKGDNSPL
                     LPLTMQYADFSIWQKGWLKGSKQQEQLNYWKKQLSGNLPVLDLPRDFSRPNKATYNGDE
                     EYITIPKGLVEKLRTLSQQEGGTLYMLLLAAYNILLYKYSKQEDIIVGTPIANRNHLEI
                     EPLIGFFVNTLALRTRL"
     CDS_motif       complement(1515..1604)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS00015_0005"
                     /evalue="3.30E-17"
                     /label="C5_LCL_267-296"
                     /locus_tag="BK733_RS00015"
                     /protein_end="339"
                     /protein_start="309"
                     /score="54.8"
                     /tool="antismash"
                     /translation="DIIVGTPIANRNHLEIEPLIGFFVNTLALR"
     CDS_motif       complement(1857..1895)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS00015_0004"
                     /evalue="9.70E-06"
                     /label="C4_LCL_164-176"
                     /locus_tag="BK733_RS00015"
                     /protein_end="225"
                     /protein_start="212"
                     /score="18.1"
                     /tool="antismash"
                     /translation="QYADFSIWQKGWL"
     CDS_motif       complement(1944..2009)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS00015_0003"
                     /evalue="3.30E-11"
                     /label="C3_LCL_132-143"
                     /locus_tag="BK733_RS00015"
                     /protein_end="196"
                     /protein_start="174"
                     /score="35.6"
                     /tool="antismash"
                     /translation="MHHIVGDGWSVGILIQEISKVY"
     CDS_motif       complement(2229..2339)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS00015_0002"
                     /evalue="2.00E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="BK733_RS00015"
                     /protein_end="101"
                     /protein_start="64"
                     /score="58.8"
                     /tool="antismash"
                     /translation="TYNMPFLLNIVGDFDIDIFEKSLNEIIQRHEILRTTF"
     CDS_motif       complement(2364..2393)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS00015_0001"
                     /evalue="3.80E-03"
                     /label="C1_LCL_004-017"
                     /locus_tag="BK733_RS00015"
                     /protein_end="56"
                     /protein_start="46"
                     /score="9.9"
                     /tool="antismash"
                     /translation="SFAQQRLWFF"
     misc_feature    complement(2488..2503)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 25.71"
                     /tool="antismash"
     gene            complement(2598..>3842)
                     /locus_tag="BK733_RS00020"
                     /old_locus_tag="BK733_00020"
     CDS             complement(2598..>3842)
                     /NRPS_PKS="Domain: PCP (309-377). E-value: 9.2e-20. Score:
                     62.6. Matches aSDomain: nrpspksdomains_BK733_RS00020_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016083252.1"
                     /locus_tag="BK733_RS00020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BK733_00020"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_140157134.1"
                     /sec_met_domain="AMP-binding (E-value: 3.3e-66, bitscore:
                     210.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.8e-12, bitscore:
                     33.8, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="PLCIGETVVLFPEGLDLEELGRWVRKRKYSVIKLTPAHLELLSHQ
                     VSEEDAAFCTDTFVVGGEPLLAKHVDFWVKHAPNTVIINEYGPTEAVVGCTNYIVKPGE
                     NYSKSIPIGKPIFNTEMYVLDTSLQPVPIGVHGELYIGGEGLAKGYYKRPELTAERFIT
                     SPFNSNEEQKLYKTGDIVRYLPNGNLEYIERIDQQVKLRGFRIELGEIESILSNHDAIS
                     SVVVNVYEENENKKLIAYVVPKYKHMMQWDFLYEYMKKHVPDYMIPAKFVVLEELPLTP
                     NGKIDRRRLPKPIFSGDDQKFVEPITDLEKEIAMIWGEVLGYKKVSMKDNFFKSGGESL
                     LATKFISLVRNKLKIELPLKIIFEVTTLEEFVLIVKQELELEQDKELIDLLKDLEDTDE
                     MMSDKVLSILINHQE"
     aSDomain        complement(2709..2912)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BK733_RS00020_PCP.1"
                     /evalue="9.20E-20"
                     /label="BK733_RS00020_PCP.1"
                     /locus_tag="BK733_RS00020"
                     /protein_end="377"
                     /protein_start="309"
                     /score="62.6"
                     /tool="antismash"
                     /translation="EKEIAMIWGEVLGYKKVSMKDNFFKSGGESLLATKFISLVRNKLK
                     IELPLKIIFEVTTLEEFVLIVKQ"
     CDS_motif       complement(3204..3269)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS00020_0002"
                     /evalue="6.00E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="BK733_RS00020"
                     /protein_end="212"
                     /protein_start="190"
                     /score="34.4"
                     /tool="antismash"
                     /translation="YIERIDQQVKLRGFRIELGEIE"
     CDS_motif       complement(3357..3446)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BK733_RS00020_0001"
                     /evalue="4.00E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="BK733_RS00020"
                     /protein_end="161"
                     /protein_start="131"
                     /score="54.2"
                     /tool="antismash"
                     /translation="PIGVHGELYIGGEGLAKGYYKRPELTAERF"
ORIGIN
        1 ccatgaacac caataggtac aggttgtaaa gaggtatcta atacatacat ttcagtatta
       61 aaaataggct ttcctatagg tatggattta ctataattct ctcctggttt tactatataa
      121 ttagtacatc caactactgc ttcagtcggt ccatattcat ttattatgac tgtatttgga
      181 gcatgcttta cccaaaaatc aacatgtttt gcgagtagag gctctcctcc tactacaaaa
      241 gtatctgtac aaaaagcagc atcttcttca gaaacttgat gagataataa ctctaaatgg
      301 gctggtgtta gctttataac actatatttt cttttccgaa cccatcttcc taattcttct
      361 aaatctaatc cctcaggaaa taaaactact gtttctccta tacataatgg tgaaagcatc
      421 cctgtaattg ttaagtcaaa agatattgat gatagaacta atgagccttg tccaccattc
      481 cctttataat aattttgagt gcaccaattt aaataatttg ttaatcctcg atgtggaacc
      541 ataactcctt ttggttttcc agtagatcct gaagtataaa ttacataagc tagattattc
      601 acttgaactt cccctattaa atccgaagtc ctcatttttt caatttcatt ccaatccata
      661 tctaaacaaa taaccttcac tttatcttta taaattctat tagataagcc ctgatgggaa
      721 agaacaattg gtaccataga gtcttctaac atataatgaa ttcttgctga tggagcattt
      781 ggatcaattg gtacatatgc tccacctgcc tttaaaaccc cgaaaattcc tataattaga
      841 tctaaagaac gttccatgga aatacctacc ataacttctg ggcctacccc ttgacctttt
      901 aaataatgag ctaactgatt agatttttta tttaattcat cataagttaa tgaagcatcc
      961 ccgtattgta ctgctatatt gttcggtact tttttgacct gttcttcaaa tagttcaggt
     1021 agagtggtat ctatctgtaa attatttatt acattatgtt ttcttttaat tatttgattc
     1081 aattcttctt tgtttattaa tgataattgg tttaatttta tgttgggatt ttcaattacc
     1141 tgttctaata tagacagata atgtttagaa atacgttgaa tggtactttt tctaaataaa
     1201 ccagtgttgt agttaaaagt tccagatacc ttatcttcct cttcaatcat agataaggtc
     1261 aaatcaaatt ttgcaacatt aatatttaat ttctcaggcc taacttttac acctgacatt
     1321 tcaattatat tttgtgtttg tttttctaaa gcaaaaacag tttgaaaaag tacagagcta
     1381 ttactctgac gttctggtac aatttctgca acaatatttt caaatgggat atcttgattc
     1441 gaatatgctc ctaggcatgt ttgttttact tcttgtaata gttccctaaa acacatatct
     1501 tttctcaatc tagtacgcaa tgctaacgta tttacaaaaa aacctattaa tggttcaatt
     1561 tctaaatgat ttcgatttgc aattggtgta cccacaataa tatcttcttg tttactatat
     1621 ttatataaca aaatattata cgccgctaat aacagcatat acaatgtacc accctcctgt
     1681 tgggataagg ttcttaattt ctctactaac cctttaggaa ttgtgatata ttcttcgtct
     1741 ccattatatg ttgctttatt aggtcttgaa aaatctcgtg gtaaatcaag cacaggtaga
     1801 ttgccactca attgtttttt ccaataattc aattgttctt gttgcttgga accttttaac
     1861 catccctttt gccaaataga aaaatcagca tattgcattg ttaatggtaa taaaggagaa
     1921 ttgtctcctt ttataaatgc attataaact ttcgaaattt cttggattaa aattccaaca
     1981 gaccatccat ctccaacaat atgatgcata tttagcaaca tccaatattc ttttccttct
     2041 acttgccaaa tatttgcacg tacaagggga cctatctcaa gattaaaagg tttttttgca
     2101 aactttttta tctgttcatc agacattttt tgtctttcct ctttagaata cgcccgtaga
     2161 tcaacatatt taacttttat tgataaattc ggatttacaa tttggattgg ctgctcgtcc
     2221 attaataaaa aagtagtgcg taaaatttca tgtctttgta taatttcatt taaacttttt
     2281 tcaaaaatat caatatcaaa gtctcctact atattcagaa gaaacggcat attatacgtt
     2341 gaagaaacgg gttctaattg atcaaaaaac caaagtcttt gctgagcaaa tgatagaaca
     2401 ggaaggtcac tttccctcct ctttttcata atatttttag ggctattccg ttttaatcga
     2461 tccattaaaa attttttctt ctctggggat aatgattcta attttgcaag tagatcttcc
     2521 tctctttcca taaacgcacc tccattatta aaaagtatat ttcgtaatat aaaactcttc
     2581 tgaaattaat aaattatcta ctcttgatga tttattaata ttgataatac tttatctgac
     2641 atcatttcat ctgtatcctc taaatctttt agtaaatcta tcaactcttt atcttgctcc
     2701 agctctagtt cttgtttcac aatcaaaaca aattcctcta aggtagtaac ttcaaatatt
     2761 atctttaatg gtaattctat tttcaatttg tttcttacta gcgaaataaa ttttgttgct
     2821 agtaaagatt ccccaccact tttaaagaaa ttatctttca tgctaacctt cttatatccc
     2881 aatacttcgc cccaaatcat tgcaatttct ttctccaaat cagttattgg ttctacaaat
     2941 ttctggtcat ccccactaaa tattggtttt ggtaatcgtc ttctatcaat tttcccattt
     3001 ggagttaatg gaagttcttc taatactaca aactttgctg gtatcatata atctggtaca
     3061 tgttttttca tatattcata aagaaaatcc cattgcatca tgtgtttata ttttggtaca
     3121 acatatgcta ttaatttttt attctcattt tcctcataaa cattaacaac tacacttgaa
     3181 attgcatcgt gattacttaa aatactttca atttctccta attcaattct aaatcctctc
     3241 aatttaactt gttgatctat gcgttcaata tactcaagat ttccatttgg taaataacgc
     3301 actatatctc cagttttata caatttttgt tcctcattac tattgaatgg agaagtaata
     3361 aatctttccg cagttaattc tggtcgttta taataccctt ttgctaatcc ctctcctcca
     3421 atatatagtt ctccatgaac accaataggt acaggttgta aagaggtatc taatacatac
     3481 atttcagtat taaaaatagg ctttcctata ggtatggatt tactataatt ctctcctggt
     3541 tttactatat aattagtaca tccaactact gcttcagtcg gtccatattc atttattatg
     3601 actgtatttg gagcatgctt tacccaaaaa tcaacatgtt ttgcgagtag aggctctcct
     3661 cctactacaa aagtatctgt acaaaaagca gcatcttctt cagaaacttg atgagataat
     3721 aactctaaat gggctggtgt tagctttata acactatatt ttcttttccg aacccatctt
     3781 cctaattctt ctaaatctaa tccctcagga aataaaacta ctgtttctcc tatacataat
     3841 gg
//
