LOCUS       NZ_NGMP01000004        11044 bp    DNA     linear   CON 19-AUG-2024
DEFINITION  Enterococcus faecalis strain 2A11_DIV0231 scaffold00004, whole
            genome shotgun sequence.
ACCESSION   NZ_NGMP01000004
VERSION     NZ_NGMP01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443294)
  AUTHORS   Earl,A., Manson,A., Schwartman,J., Gilmore,M., Abouelleil,A.,
            Cao,P., Chapman,S., Cusick,C., Shea,T., Young,S., Neafsey,D.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genomics Platform, The Broad Institute Genomic
            Center for Infectious Diseases
  TITLE     The Genome Sequence of Enterococcus faecalis 2A11_DIV0231
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 443294)
  AUTHORS   Earl,A., Manson,A., Schwartman,J., Gilmore,M., Abouelleil,A.,
            Cao,P., Chapman,S., Cusick,C., Shea,T., Young,S., Neafsey,D.,
            Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genomics Platform, The Broad Institute Genomic
            Center for Infectious Diseases
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2017) The Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: allpaths v. R48559
            Genome Coverage                   :: 116.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002141205.1-RS_2024_08_19
            Annotation Date                   :: 08/19/2024 03:18:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,819
            CDSs (total)                      :: 2,752
            Genes (coding)                    :: 2,710
            CDSs (with protein)               :: 2,710
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 4, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 4 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 24 of 42
            Pseudo Genes (incomplete)         :: 18 of 42
            Pseudo Genes (internal stop)      :: 7 of 42
            Pseudo Genes (multiple problems)  :: 5 of 42
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:20:32
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 331051
            Orig. end                         :: 342095
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NGMP01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11044
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11044
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11044
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6044](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6044
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            <142..396
                     /locus_tag="A5830_RS13135"
                     /old_locus_tag="A5830_002512"
                     /pseudo=""
     CDS             <142..396
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401933.1"
                     /locus_tag="A5830_RS13135"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="A5830_002512"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="VLAFFLNIEDAICLYKKKAYTNGIKEIGYVSKSSQLAISKEKNII
                     INEMLVPEQQLTNGLGINLRKCLLMEALILEVLTNDIHE"
     gene            380..1357
                     /locus_tag="A5830_RS13140"
                     /old_locus_tag="A5830_002513"
     CDS             380..1357
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365263.1"
                     /locus_tag="A5830_RS13140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A5830_002513"
                     /product="hypothetical protein"
                     /protein_id="WP_002399541.1"
                     /transl_table=11
                     /translation="MIFTNKSSYGVKSDMFLSNINIGYVNFSRVKIKQGTTAGSAFSVD
                     ENKQLSLIKSYNEYLERLFLGIPLSTNKTISSVNLEKNIVSSRKYSEFGYGNHEFGFND
                     TTGTSSGKLSEVIIQKALTELIEKNEVFCFWYGLKGEKLKQDKEIHDLINDYNFISNMF
                     KIYVVRELSNYSTVIVMGFLEGKLLTSGVCCSITMEQSLILALKEAKGIEWQTFNNPLA
                     KANKFSDKLHKQILKKVKFMDETYKVITKDSIQATKYIETADWINHVEISVIGDDINRG
                     VKTIKCISKQLLNSVPIKINIERQKDKEIIKRYLIDYSIDCPIQ"
     gene            1375..1857
                     /locus_tag="A5830_RS13145"
                     /old_locus_tag="A5830_002514"
     CDS             1375..1857
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A5830_RS13145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A5830_002514"
                     /product="hypothetical protein"
                     /protein_id="WP_256924928.1"
                     /transl_table=11
                     /translation="MKGFLRNKVNFVLNEDLFMSHHYNVYIEKYSKRPIKAYEVDLKNK
                     RQHILSTLGEYLERETLINENSPKEHTVIATNLIDGSIKQVNLDMILFKNSFVDSSGMA
                     SHKKSKPLIWTAYKEFFERQSYITTHLDVYKRQGMASHKKSKPLIWTCLLYTSRCV"
     gene            2275..2478
                     /locus_tag="A5830_RS13150"
                     /old_locus_tag="A5830_002515"
     CDS             2275..2478
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405754.1"
                     /locus_tag="A5830_RS13150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A5830_002515"
                     /product="hypothetical protein"
                     /protein_id="WP_002410046.1"
                     /transl_table=11
                     /translation="MNVEEVLVSQKTPNAINEETELYGFGYGSVVENITVSKGGGGGGG
                     GGGGGCCCCCCCCCGGATGLEE"
     misc_feature    2491..2502
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            2543..3373
                     /locus_tag="A5830_RS13155"
                     /old_locus_tag="A5830_002516"
     CDS             2543..3373
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04424"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379875.1"
                     /locus_tag="A5830_RS13155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A5830_002516"
                     /product="McbB family protein"
                     /protein_id="WP_002365265.1"
                     /sec_met_domain="TIGR04424 (E-value: 3.8e-89, bitscore:
                     293.7, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYKVNPFIWHDLSEKETFFQTKLSSIIITNKNLVRMLKDLEKNEV
                     INFEESLLSNYFRSNMINNVLVFLEENKLIKKLDIENNKVNEVLVYTDDSEFSELFDYV
                     FSEKFTISHQSSISHLESKNMDGKLLIVFLKAFNFKEYENIVNIAKEKNVVIKIIFSYN
                     HHIYFSNFYKREWYNPCPICFFSELESQLRGEITSDTANFQTIIDLLYTKSQNFAIEYP
                     LQKDDYFTIVYTIMRNLDSDLMKCDLDEVLSIDLNNHLVTKDIAYHWGYCDCYE"
     gene            3366..4022
                     /locus_tag="A5830_RS13160"
                     /old_locus_tag="A5830_002517"
     CDS             3366..4022
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401933.1"
                     /locus_tag="A5830_RS13160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A5830_002517"
                     /product="hypothetical protein"
                     /protein_id="WP_010823210.1"
                     /transl_table=11
                     /translation="MSDYYWNEYKNVLLDEAYLIDTLKYQQRVVNKQVEENNLDELSDV
                     LGLYNVKYSDIRLAVYPFDELPVFIKTLVSDLQKNYASFIYKVSMPILAITKKIGNVNT
                     GCYSFDFKNNFLILYETNVTKIREIVNDKLVLAFFLNIEDAICLYKKKAYTNGIKEIGY
                     VSKSSQLAISKEKNIIINEMLVPEQQLTNGLGINLRKCLLMEALILEVLTNDIHE"
     gene            4006..4983
                     /locus_tag="A5830_RS13165"
                     /old_locus_tag="A5830_002518"
     CDS             4006..4983
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365263.1"
                     /locus_tag="A5830_RS13165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A5830_002518"
                     /product="hypothetical protein"
                     /protein_id="WP_002399541.1"
                     /transl_table=11
                     /translation="MIFTNKSSYGVKSDMFLSNINIGYVNFSRVKIKQGTTAGSAFSVD
                     ENKQLSLIKSYNEYLERLFLGIPLSTNKTISSVNLEKNIVSSRKYSEFGYGNHEFGFND
                     TTGTSSGKLSEVIIQKALTELIEKNEVFCFWYGLKGEKLKQDKEIHDLINDYNFISNMF
                     KIYVVRELSNYSTVIVMGFLEGKLLTSGVCCSITMEQSLILALKEAKGIEWQTFNNPLA
                     KANKFSDKLHKQILKKVKFMDETYKVITKDSIQATKYIETADWINHVEISVIGDDINRG
                     VKTIKCISKQLLNSVPIKINIERQKDKEIIKRYLIDYSIDCPIQ"
     gene            5001..6044
                     /locus_tag="A5830_RS13170"
                     /old_locus_tag="A5830_002519"
     CDS             5001..6044
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401935.1"
                     /locus_tag="A5830_RS13170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A5830_002519"
                     /product="YcaO-like family protein"
                     /protein_id="WP_010711509.1"
                     /sec_met_domain="YcaO (E-value: 1.5e-07, bitscore: 26.0,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGFLRNKVNFVLNEDLFMSHHYNVYIEKYSKRPIKAYEVDLKNK
                     RQHILSTLGEYLERETLINENSPKEHTVIATNLIDGSIKQVNLDMILFKNSFVDSSGMA
                     SHKKSKPLIWTAYKEFFERQSYITNFLFHTKAQQLNIDKIEEIKKIDRYLTNYLDNVKY
                     YNISLSKNLYVVLAVGYGEKYKAVGLGTSQSARKAVIKSQKEILQYFATSHSKYKEGKV
                     SQGSGLVIDNKDIYHDYFDRLSSKEVKDLYAYLEGSEFIILNNEEKIEKVSHTFTIKNI
                     SNSLNMYPYITMFENNHAKNVKVVKVFDPKWFPNMAPRNFSKEIILNVENILKLKRKNF
                     SELLPFP"
     gene            6057..6737
                     /locus_tag="A5830_RS13175"
                     /old_locus_tag="A5830_002520"
     CDS             6057..6737
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405757.1"
                     /locus_tag="A5830_RS13175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A5830_002520"
                     /product="hypothetical protein"
                     /protein_id="WP_002365261.1"
                     /transl_table=11
                     /translation="MFISVKSYALFNIKMLLKDKIPLLWSIALPVITFFMNYRSVTNEW
                     ELAYWWVYIVVCSYVYGVGVYALQLKEYGSLKTIFSINNSPWAFFLGNLLTQIMFSFIS
                     VNLLNLIVVILLPLSFLRLFLYSFITIIACLPVAFFSYNLTLIKKVHANTINSIFTMLI
                     FGLFILLNTNIELNKYNPLSILSNVLIEHKNMDVFKYVTFSIFLVFISMYSIVKFEPIS
                     NERR"
     assembly_gap    6777..6876
                     /estimated_length=unknown
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6877..7721
                     /locus_tag="A5830_RS13180"
                     /old_locus_tag="A5830_002521"
     CDS             <6881..7721
                     /codon_start=3
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /locus_tag="A5830_RS13180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A5830_002521"
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="WP_256924929.1"
                     /transl_table=11
                     /translation="NGEKVEDNDVWKDNVLNVKQTIKAGESVTITFKVKAIKAAEGQTI
                     ANVAETDDSNVPPTPPAETKVPIEPTIHKNIEGVQKLILKNNKQKFNWNITVSFGTNTM
                     NWKQASITDSVNNLLDIVKVTVVDENGENVTGNGTLSTENNKVIFEINKKDDSYTYLAG
                     HTYTMIITTKIKDSVTDKELAPYIKDGGVPNQAYLNFGNEGDKKHSEIPKIVPLKGASP
                     EKTNTPSNQTDKGNLTPSKGIFPHTGENQMLSNILLVVGTCILIILTVYYILKKRRIE"
     misc_feature    8124..8137
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: weak, score: 15.27"
                     /tool="antismash"
     gene            8595..9716
                     /locus_tag="A5830_RS13185"
                     /old_locus_tag="A5830_002522"
     CDS             8595..9716
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /locus_tag="A5830_RS13185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A5830_002522"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_256924930.1"
                     /transl_table=11
                     /translation="MNFNVFLDEDQRYKYYIVKLLELKQNTFFSQAQLMEALGVSKYKI
                     DRFLKDIEEDSELLNLKISIVVDVSGEIRSQGFNNLVTKKLRLYYLEQSKQYQLLYEML
                     VGSNQSAESLATKLYVSRASIYNVLKILKDNLKIYGLKVKNLKIVGRELKVRSFFFSLF
                     NDFYCGITKPFSQELNKRINSFKNLLLVNKNIYPTKVQEYRLELFIGISFIRKEKNFLL
                     DESVLDIPELDLYPYFKLYGSSKNKSNIDIQNEIQNILFFCYTENLISDPQWEINFKDN
                     KEALDISENFVKELEKVVTLDIKFKEQIGKEVFRINQKWLYFHFKEATFVFENQKSYFQ
                     EIYFKCDSIVRTYLGQSSLEKLFEDKELSLIHI"
ORIGIN
        1 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
       61 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      121 nnnnnnnnnn nnnnnnnnnn ngtattagct ttttttttaa acatagagga tgcaatttgt
      181 ttatataaaa aaaaggcgta cacaaatggt ataaaagaaa ttggttatgt ttctaaaagc
      241 agtcaattag cgattagtaa agaaaaaaac ataataataa acgaaatgct agtaccagag
      301 caacaactga caaatggatt aggtataaac ttgagaaaat gtttgttaat ggaagcttta
      361 attttagagg tgttaactaa tgatattcac gaataaaagt tcctacggtg tgaaaagtga
      421 tatgttttta tcaaatataa atattggata tgtgaatttt tcacgtgtca agattaagca
      481 aggaacgaca gcaggtagtg ctttctcagt tgacgaaaat aaacagttat cacttattaa
      541 aagttataat gaatatttag aaagactttt cttagggata ccattaagta caaataaaac
      601 tatttcttct gtaaatttag aaaaaaatat tgtaagtagc agaaaatatt cagaatttgg
      661 atatggaaat catgagtttg gatttaatga tacgacagga acttcatctg gaaaactttc
      721 agaagtaata atccaaaagg cactaacaga attgattgag aaaaatgaag tattttgttt
      781 ttggtatggt ttgaaagggg aaaagttaaa acaagataaa gaaattcatg acttaattaa
      841 tgattataat tttatttcaa atatgtttaa aatttatgta gttcgtgaac ttagtaatta
      901 ttcaacagtg atcgtgatgg ggtttttaga agggaaatta ctaacctcag gtgtatgttg
      961 tagtattact atggaacaat ctctaatttt ggccttgaaa gaagctaagg gaatagagtg
     1021 gcaaactttt aataatccct tagcaaaagc aaataaattt tctgataaat tgcataaaca
     1081 aattttaaaa aaagtaaagt ttatggatga aacatacaaa gttattacaa aagattctat
     1141 acaagctacg aaatacattg aaactgctga ttggattaac catgttgaga tatccgtaat
     1201 tggagatgac ataaatagag gagttaaaac aataaaatgt atatccaaac agttactaaa
     1261 ttctgtacca attaagataa acattgaaag acaaaaagat aaagagatta ttaagagata
     1321 tttaattgat tattctattg attgtcctat ccaatgatag aaaggagaat acttttgaaa
     1381 ggttttttaa gaaataaggt gaattttgtt ttaaatgagg atttatttat gtcacatcac
     1441 tataacgtat atatagaaaa atattctaaa agacctatca aagcctatga ggttgattta
     1501 aagaataaaa ggcagcatat tctttcaact ttaggagagt atttggagcg agaaacactc
     1561 atcaacgaaa attcaccaaa agagcatacg gttattgcta cgaatttaat tgatggaagc
     1621 attaaacaag ttaatcttga tatgattttg tttaagaata gttttgttga ttcttctggt
     1681 atggcctcgc ataaaaaatc aaagccatta atatggacag cgtataaaga attctttgaa
     1741 agacagagct atattactac acatctagat gtgtataaga gacagggtat ggcctcgcat
     1801 aaaaaatcaa agccattaat atggacctgt ctcttataca catctagatg tgtataagag
     1861 aacagagcta tattactaac ttcttgtttc atactaaggc tcagcaatta aatatagata
     1921 agatagaagc tgtctcttat acacatctag atgtgtataa gagacagtta ttaaagaggt
     1981 aaaatccgaa aatatgcgaa tatgcattgt atattataat tttaatgtga taaaataaat
     2041 attttaaaaa ttatttttgt tagaattttc attgatataa aaatagaaaa attaatgata
     2101 gttgttttgt tattaatttt tttgtttttg ttgtctaatc aaagaatttt agatttttca
     2161 gtataaaaat acgtttttta attagaataa gatgagtttt tgcttgaaaa ttgattggat
     2221 agtggtaaaa taatttcatc aatattgata aaattataac ggaggtgtat taaaatgaat
     2281 gttgaagaag ttttagtgtc gcaaaaaacc ccaaatgcaa ttaatgaaga gactgaatta
     2341 tatgggtttg ggtatggttc tgtcgttgag aacattactg tctctaaagg tggaggcggt
     2401 ggtggaggcg gcggtggcgg tggctgttgt tgttgttgct gctgttgttg tgggggggcc
     2461 acaggattag aagaatagaa acttcataag gtaagtggac gcacttacct ctttacatac
     2521 tcaagtaaaa aggagtgaaa aaatgtataa agtaaaccct tttatatggc atgatcttag
     2581 tgaaaaagaa acattctttc aaacaaaact atcttcgata attattacta ataaaaattt
     2641 agttagaatg ttaaaagatt tagaaaaaaa tgaagtaata aattttgagg aatctctatt
     2701 atccaattat tttaggagca acatgattaa taatgtactg gtctttttag aagaaaataa
     2761 attaattaaa aagctagata tagaaaataa taaggttaat gaggttttgg tatataccga
     2821 tgattctgaa ttttctgaat tattcgatta cgtgttttcc gaaaaattta caattagtca
     2881 tcagagcagt atttcccact tagaatctaa aaatatggat ggtaaattgc ttatagtatt
     2941 tttaaaggct ttcaatttta aagaatatga aaatattgta aatatagcca aggaaaagaa
     3001 tgtagtcatt aaaataattt ttagctataa tcatcatatt tatttttcga atttctataa
     3061 aagggaatgg tacaatccat gtccaatatg ttttttttct gaactagaaa gccaattaag
     3121 aggagaaata acttctgata cagctaattt tcagacaatt atagatttac tttatacaaa
     3181 atcacaaaat tttgctatag aatacccgtt gcaaaaggat gattatttta cgatagttta
     3241 taccataatg aggaatttgg atagcgattt aatgaaatgt gacttagatg aagttttatc
     3301 tattgattta aataatcatt tagttacaaa agatattgct tatcattggg ggtattgtga
     3361 ttgctatgag tgattattat tggaatgaat ataagaatgt actattagat gaagcatatt
     3421 taattgatac gttaaaatat caacaaaggg tagtgaataa acaagtagaa gagaataatt
     3481 tagatgaatt gagcgatgtt ttaggtcttt ataatgtgaa atactcggat attaggttag
     3541 cggtatatcc ttttgatgag ttacctgtct ttataaaaac gttagttagt gatttacaaa
     3601 aaaattatgc ttcctttata tataaagtta gtatgccaat actagccata acaaaaaaaa
     3661 tagggaatgt aaatactggt tgttattctt ttgattttaa aaataatttt ttgattctat
     3721 atgaaactaa tgtaactaag attagagaaa tagtaaatga taaactggta ttagcttttt
     3781 ttttaaacat agaggatgca atttgtttat ataaaaaaaa ggcgtacaca aatggtataa
     3841 aagaaattgg ttatgtttct aaaagcagtc aattagcgat tagtaaagaa aaaaacataa
     3901 taataaacga aatgctagta ccagagcaac aactgacaaa tggattaggt ataaacttga
     3961 gaaaatgttt gttaatggaa gctttaattt tagaggtgtt aactaatgat attcacgaat
     4021 aaaagttcct acggtgtgaa aagtgatatg tttttatcaa atataaatat tggatatgtg
     4081 aatttttcac gtgtcaagat taagcaagga acgacagcag gtagtgcttt ctcagttgac
     4141 gaaaataaac agttatcact tattaaaagt tataatgaat atttagaaag acttttctta
     4201 gggataccat taagtacaaa taaaactatt tcttctgtaa atttagaaaa aaatattgta
     4261 agtagcagaa aatattcaga atttggatat ggaaatcatg agtttggatt taatgatacg
     4321 acaggaactt catctggaaa actttcagaa gtaataatcc aaaaggcact aacagaattg
     4381 attgagaaaa atgaagtatt ttgtttttgg tatggtttga aaggggaaaa gttaaaacaa
     4441 gataaagaaa ttcatgactt aattaatgat tataatttta tttcaaatat gtttaaaatt
     4501 tatgtagttc gtgaacttag taattattca acagtgatcg tgatggggtt tttagaaggg
     4561 aaattactaa cctcaggtgt atgttgtagt attactatgg aacaatctct aattttggcc
     4621 ttgaaagaag ctaagggaat agagtggcaa acttttaata atcccttagc aaaagcaaat
     4681 aaattttctg ataaattgca taaacaaatt ttaaaaaaag taaagtttat ggatgaaaca
     4741 tacaaagtta ttacaaaaga ttctatacaa gctacgaaat acattgaaac tgctgattgg
     4801 attaaccatg ttgagatatc cgtaattgga gatgacataa atagaggagt taaaacaata
     4861 aaatgtatat ccaaacagtt actaaattct gtaccaatta agataaacat tgaaagacaa
     4921 aaagataaag agattattaa gagatattta attgattatt ctattgattg tcctatccaa
     4981 tgatagaaag gagaatactt ttgaaaggtt ttttaagaaa taaggtgaat tttgttttaa
     5041 atgaggattt atttatgtca catcactata acgtatatat agaaaaatat tctaaaagac
     5101 ctatcaaagc ctatgaggtt gatttaaaga ataaaaggca gcatattctt tcaactttag
     5161 gagagtattt ggagcgagaa acactcatca acgaaaattc accaaaagag catacggtta
     5221 ttgctacgaa tttaattgat ggaagcatta aacaagttaa tcttgatatg attttgttta
     5281 agaatagttt tgttgattct tctggtatgg cctcgcataa aaaatcaaag ccattaatat
     5341 ggacagcgta taaagaattc tttgaaagac agagctatat tactaacttc ttgtttcata
     5401 ctaaggctca gcaattaaat atagataaga tagaagaaat aaaaaaaata gacagatatt
     5461 tgactaatta tttagacaat gtcaaatatt ataatatatc tttaagtaag aacctttacg
     5521 tggtacttgc cgttgggtat ggtgaaaaat acaaagcagt gggtttggga actagtcaat
     5581 cagcaagaaa agcagtaata aaatcacaaa aagaaatatt acaatatttt gctacatcgc
     5641 attcaaaata taaagaagga aaagttagtc aaggctctgg attagtaatt gacaataagg
     5701 acatttatca tgactatttt gatagattga gctcgaaaga agttaaagat ttatatgcat
     5761 atcttgaagg tagtgaattt attattctga ataatgaaga gaaaatagaa aaagttagtc
     5821 atacatttac tatcaaaaat ataagtaatt ctcttaatat gtatccgtat attacaatgt
     5881 ttgaaaataa tcatgctaag aatgtgaaag tagtgaaggt atttgaccca aagtggttcc
     5941 ccaacatggc accaagaaat ttttcgaaag aaataatatt aaatgtagag aatatattaa
     6001 aattaaaaag aaaaaatttt tcagaactgt taccatttcc gtaaggagga gtgaaaatgt
     6061 ttatctcagt taaatcatat gcattgttta atattaaaat gttactgaaa gataaaattc
     6121 ctttattgtg gtcaatagcc ttacctgtaa ttactttttt tatgaattat agaagtgtaa
     6181 caaatgaatg ggaattggca tattggtggg tatatattgt tgtttgttcc tatgtttatg
     6241 gagtaggagt ttacgcatta caattaaagg aatatggaag cttaaaaaca attttttcaa
     6301 taaataattc accttgggca ttttttttag gcaatctatt gacgcaaatt atgtttagct
     6361 ttatcagtgt aaacttactt aatttaattg tggttatttt actaccatta tcgtttctcc
     6421 gtcttttttt atatagcttt attactatta tagcgtgttt accagttgca ttttttagtt
     6481 acaatttaac tctaataaaa aaagtgcatg ctaatactat aaattcaata tttacaatgc
     6541 taatttttgg tttatttata ttgttaaata cgaatataga gttaaataaa tataaccctc
     6601 tgtcaatttt atctaatgtt ttaattgaac ataaaaatat ggatgtcttc aaatatgtta
     6661 ctttttcaat atttttagtt tttattagta tgtattctat agttaaattt gaaccgattt
     6721 caaatgaaag aaggtaaagc tatgatcgaa ataaaggatt taaatattcc ttatgtnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaaa tggtgaaaaa gtagaagata
     6901 atgatgtctg gaaagacaat gtattaaatg taaaacaaac aattaaagct ggagaaagtg
     6961 tcacaattac gtttaaagta aaagcaatca aagcagcaga aggtcaaacg attgcgaatg
     7021 tggcagaaac agatgattca aacgtaccac caactccacc agcagaaaca aaagtaccta
     7081 ttgaacctac tattcacaag aatatagaag gtgtacagaa gttaatacta aagaataata
     7141 aacagaaatt taattggaat attactgttt cttttggaac caatactatg aattggaaac
     7201 aagcttccat tacagactca gtaaataatt tattagacat tgtgaaagtt acagtagtgg
     7261 atgaaaatgg cgaaaatgtg actgggaacg gtacactgag tacagaaaac aacaaagtca
     7321 tttttgaaat taataaaaaa gatgacagct atacttattt ggcaggtcat acgtatacca
     7381 tgataattac cacaaaaatc aaagatagtg taacggataa agagttggct ccatatatta
     7441 aagatggtgg agtgccaaac caagcatatt taaactttgg taatgaagga gataaaaagc
     7501 attcagaaat acctaaaata gttccgctaa aaggagcttc tccagaaaaa actaatactc
     7561 caagtaatca aactgataaa ggtaatctta ctccttcaaa aggtattttc ccacatactg
     7621 gagaaaatca aatgctaagt aatatactgc ttgttgtagg gacatgtatt ttaataatcc
     7681 ttacagtata ttatattctt aaaaaaagac gaattgaata gttaatttgt agtatagtca
     7741 gagtaggttt gtattacttg atattatata agctgttcta atagttatta aggttaggtt
     7801 gtatagctgt ctcttataca catctagatg tgtataaaag aaatgaatta taataagctc
     7861 agaccgtagt ttaaattata gtaaatataa aataattatg tttttgtata gtagtttttt
     7921 ggaaagtata gatagtactg gttgatttgc aatgtttttt ttgcaaagta attaagttca
     7981 aaaagtaatg atttctagct tttgcgttag tggtgcttgc tttttattaa agcaagcaaa
     8041 ctgtggaggt caagtctcta ttaaaataat aggaatctta attttaattt ctatattgga
     8101 gttgaataaa aataataaaa aaaggaacag tcattgcata gtgcgccccc aaagttagat
     8161 tttttagtct aactttgggg gcgcactaca ttgttaatag ataactgttc cttttttatt
     8221 tagtaaatga attttcgctt gtacataata atataaagaa gacataaaaa ctagtttccg
     8281 ctagttttta tatcagatag attgtaatag aagttatgaa cttctaccaa ataattatag
     8341 caaaaaattt tgatcatgtt gaataaatac ttactgtcat tctgcttttt ttgacgtgaa
     8401 taaaaaataa tagacatctt taaaggtaat tagtattgaa aatttgcagt attgtaagca
     8461 cgacattaca ataaattgac ttagtctaaa aaattttttg tgcctgaaaa atatatagaa
     8521 aaaaacgatt attttgtata gaaaagactg attaattaaa ctaatattgg ttgagtagaa
     8581 atggagctgt agttatgaac tttaatgttt ttttagatga agatcaaaga tacaaatatt
     8641 atattgtaaa gttgcttgag ttgaaacaaa atactttttt ttctcaagca caattgatgg
     8701 aggcattagg agtttcaaaa tataaaatag atagattttt aaaagatatt gaagaagata
     8761 gtgaattatt aaacttgaaa atcagcatag ttgtagacgt ttcaggagaa ataagatcac
     8821 aaggttttaa taacttagta actaaaaaat tacgattgta ttatttggaa cagagcaagc
     8881 aatatcaatt attatatgaa atgttagttg gtagcaatca aagtgcggaa tcattagcta
     8941 ctaaattata tgttagccga gcttctattt acaatgtatt aaaaatcttg aaagataatt
     9001 taaaaattta cggacttaaa gttaaaaatt tgaaaatagt aggacgcgag ttaaaggtac
     9061 gctccttttt tttctcgcta tttaatgatt tttattgcgg aataactaag cctttctctc
     9121 aagaactaaa taaaagaatt aatagcttca aaaatttatt attagtaaat aaaaatattt
     9181 atcctacaaa agtacaagaa tatcgacttg aactttttat tggtatttcc tttatacgaa
     9241 aagaaaaaaa ttttttgcta gatgaatctg tattagatat tcctgagttg gatttgtatc
     9301 cctattttaa gttatacggt agcagcaaaa ataaatctaa catagatatc caaaacgaaa
     9361 tacaaaatat attatttttt tgttatacag agaacttgat tagcgatcca caatgggaga
     9421 taaattttaa agataataaa gaagcattag atattagtga aaactttgta aaggaattag
     9481 aaaaagttgt tactttagac attaaattta aagaacaaat aggaaaagaa gtatttagaa
     9541 ttaatcagaa gtggttatat tttcacttta aagaggcaac ttttgtattc gagaatcaaa
     9601 aaagctattt tcaagaaata tattttaaat gtgattcaat tgtacgtacg tatttaggtc
     9661 agagttcgtt agaaaaatta tttgaagata aagaactgtc tcttatacac atctagatgt
     9721 gtataagaga cagaagatgg agaatctgcc cagcaaagtt ctacaaatag tgaatctagt
     9781 aattcattag atattaatag tttaaataaa gagcaaaaaa atgaagtgga tatacacatc
     9841 tagatgtgta taagagacag atatattgga aaagaatact tttataatta cgaatattta
     9901 atttatcaaa gaatattacc tgtaaatcat ccgtacacga ttgaacaaac atacaccctg
     9961 aatgtttgtt caattgtgta tttttagtat gtataatatt aggatgtatt aaatagctta
    10021 caaaattgat atatgtttaa atctttttat ctattgtatt agggctacgc ttcaagtata
    10081 gtagttgatg ttctaaaatt ttgttgattt cctacttcgt atttgaatcc tatctttatt
    10141 ggaatcttat ttttggtata tacaactcaa aatatagctg ataagaagga gaatttatat
    10201 gaaactagat aaattaaaaa atcttgtatt aagccaaacg caattaaaga gcttggttgt
    10261 agtttataat ctgtatccta gaggcaaaca aatgaatgag ctagaaaatc tctgccaagt
    10321 ttctaaaaat gaattaaaga gagtcctaaa agtattggat gaagaagtca aaagccttca
    10381 cttctttgat aagattgaca atatactagt ggttaaagat aagtttgttt catttaattc
    10441 tgaaataaat gaagcaactt atacaatgtt gcttatgcag ttaagaaaaa aatatttact
    10501 ttcttcaagt gtttacaaag cattgctatt tgtattagag caacgctatt tttgtttgca
    10561 agaattagct gatcacttgt tctatagtga ttcttatact tataaaataa taaaaagatt
    10621 gaaaaatttt ttctattact tagaaacaga tttaaaatta gtgaaagtta ataaatctga
    10681 tctctatctt gaggggaggg aatcttctaa aataattttc cactatttat tagtcatgtt
    10741 ggtttcccat gaaaatgaat gggtatttca agacagtgaa aaagaagtgg tgttatctta
    10801 ccagcccaca aaaattttag atagcttaga gttactaagc gactgtaata gagataaaat
    10861 tttgaatctt ttaacaatct ataaaataac ctctcacgtt aaggacccct tactttgcct
    10921 atctaaaaat attattgaaa taggtgaact gatggataac gatggtgaat ttccatttta
    10981 tataaatcaa actgtcgacg tattaaatga tgatcgaaac attcatttca tattcatggt
    11041 tcat
//
