LOCUS       NZ_CP021121            10396 bp    DNA     linear   CON 31-JUL-2024
DEFINITION  Streptomyces marincola strain SCSIO 03032 chromosome, complete
            genome.
ACCESSION   NZ_CP021121
VERSION     NZ_CP021121.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces marincola
  ORGANISM  Streptomyces marincola
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6287975)
  AUTHORS   Ma,L., Zhu,Y., Zhang,W., Zhang,G., Tian,X., Zhang,S. and Zhang,C.
  TITLE     Complete genome sequence of Streptomyces sp. SCSIO 03032 revealed
            the diverse biosynthetic pathways for its bioactive secondary
            metabolites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6287975)
  AUTHORS   Ma,L., Zhu,Y., Zhang,W., Zhang,G., Tian,X., Zhang,S. and Zhang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2017) Key Laboratory of Tropical Marine
            Bio-resources and Ecology, South China Sea Institute of Oceanology,
            164 West Xingang Rd., Guangzhou, Guangdong 510301, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method :: Hierarchical Genome Assembly Process v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 165.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002128305.1-RS_2024_07_31
            Annotation Date                   :: 07/31/2024 06:55:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,513
            CDSs (total)                      :: 5,438
            Genes (coding)                    :: 5,343
            CDSs (with protein)               :: 5,343
            Genes (RNA)                       :: 75
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 19 of 95
            Pseudo Genes (incomplete)         :: 88 of 95
            Pseudo Genes (internal stop)      :: 2 of 95
            Pseudo Genes (multiple problems)  :: 13 of 95
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:29:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5392315
            Orig. end                         :: 5402711
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP021121.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Key Laboratory of Tropical
            Marine Bio-resources and Ecology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10396
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="26"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10396
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10396
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5396](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5396)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(103..116)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.86"
                     /tool="antismash"
     misc_feature    103..116
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.71"
                     /tool="antismash"
     gene            complement(273..1304)
                     /locus_tag="CAG99_RS23490"
                     /old_locus_tag="CAG99_23555"
     CDS             complement(273..1304)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1078: LacI family
                     transcription regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433884.1"
                     /locus_tag="CAG99_RS23490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CAG99_23555"
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_086162447.1"
                     /transl_table=11
                     /translation="MSPTNNATRRRRPTIHDVAREAGVSRGTVSRVLNGGRHVSPAAAE
                     AVNAAIRRTGYVVNRHARSLITGRSGSVAFLLTEPQERFFEDPNLGVLLRGCTQALAAH
                     DIPLLLMIAGSGAEQRRITRYVSAGHVDGVLLVSSRTGDPMAETLVRAGLPVVMCGRPV
                     GRATGLAHVAADDRGGARQMVRHLLHTGRRRVATVTGPLDTPGGVERLAGYREVLAEAG
                     LPADEDLVETGDYSHDGAAAATARLLARRPDLDALFVASDLMARGALAALERAGRRVPH
                     DVAVGGFDDSTAAVASRPGLTTVRQPWDRISSEMVRVLLARMAGEDTSAVILPTELVRR
                     ESA"
     gene            1454..3454
                     /locus_tag="CAG99_RS23495"
                     /old_locus_tag="CAG99_23560"
     CDS             1454..3454
                     /GO_component="GO:0009341 - beta-galactosidase complex
                     [Evidence IEA]"
                     /GO_function="GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174265.1"
                     /locus_tag="CAG99_RS23495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CAG99_23560"
                     /product="beta-galactosidase"
                     /protein_id="WP_086162448.1"
                     /transl_table=11
                     /translation="MDRLAFGGDYNPEQWPEELWPEDVALMREAGVTLVSIGIFSWARL
                     EPREGAYDFGWLDRIIDLLHEGGIRIDLATPTVVPPAWFYRAHPEALPVTREGVRLEFG
                     SRGAICHSSPAYREAAARITRALGERYGDHPALALWHVHNEYGVPVLECYCDVSAAHFR
                     RWLAERYGTLDALNAAWGTAFWGQAYGEWDEVQPPRLTPTTANPAQRLDFARFASDSAL
                     ENFRAERDLLHELSPGVPVTTNFMVSPTQCDTIDYWKWGREVDIVTNDHYLVAEDDRNH
                     INLSFAADLTRSVGAGRPWLLLEHSTSAVNWQPRGIAKRPGEMARNSLAHVARGSQGAM
                     FFQWRASRAGAEKFHSAMVPHAGTDSRVWREVVELGGHIDALADIMDTRTVADAAVVWD
                     WESWWAHKLEWRPSDDLGTRERAEAWYAALFDQHLTVDFAHPESGDLAGRPLVVVPALY
                     LATRAAGENLRRYVEGGGTLVVSCFSGIVDEHDAVHPGPHPGALRDVLGLVVEEFQPLR
                     AGETVRLAGPEGATATADVWAETVRTTGAETVWTYADGPAAGGPAITRHRLGDGTAWYV
                     SARVGQEGLRQVLAAAGKDAGLDPRDDLPRDVEVVRRAGPGGDFLFAINHTAEDHEVAL
                     PASGTELLTGTPVTGPLAVPAGATRVVRLDR"
     misc_feature    1473..1492
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.54"
                     /tool="antismash"
     gene            complement(3529..4002)
                     /locus_tag="CAG99_RS23500"
                     /old_locus_tag="CAG99_23565"
     CDS             complement(3529..4002)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085944.1"
                     /locus_tag="CAG99_RS23500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CAG99_23565"
                     /product="AAA family ATPase"
                     /protein_id="WP_255308302.1"
                     /transl_table=11
                     /translation="MAKVLLTGMSGTGKSAALRLLRARGHRTVDTDSDAWSRWVTLPDG
                     SSDWIWREREITGLLTGHAAGHLFLAGCKSNQGDFYPLLDHVVLLSAPAHVLLARIERR
                     TGNPYGKRPEERAAVLRHLATVEPLLRATASAEIDATAPIEEVVGRLERLAGS"
     misc_feature    complement(4003..4021)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.25"
                     /tool="antismash"
     misc_feature    4013..4024
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: strong, score: 20.58"
                     /tool="antismash"
     gene            complement(4081..4980)
                     /gene="thpD"
                     /locus_tag="CAG99_RS23505"
                     /old_locus_tag="CAG99_23570"
     CDS             complement(4081..4980)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358305.1"
                     /locus_tag="CAG99_RS23505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CAG99_23570"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_086161248.1"
                     /sec_met_domain="PhyH (E-value: 8.6e-38, bitscore: 129.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGATEVTTPRQDPVVWSEPGAPGPFSTDELA
                     GFERDGFAAVDQLITPEEVLAYRAELDRLVTDPDIRADERSIVEPSSQEIRSVFEVHKI
                     SEKFAGLVRDPRLLDRARQILGSEVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     RMRTVSVSIALTENFDTNGGLMIMPGSHLSFLGCAGETPKDNYKKSLQMQDAGTPSDEA
                     LTKFADQHGIKLFTGRAGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENAAVEPFA
                     APVRRPEFIGARDFTPVS"
     gene            complement(5001..5396)
                     /locus_tag="CAG99_RS23510"
                     /old_locus_tag="CAG99_23575"
     CDS             complement(5001..5396)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358304.1"
                     /locus_tag="CAG99_RS23510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CAG99_23575"
                     /product="ectoine synthase"
                     /protein_id="WP_086161249.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.5e-53, bitscore:
                     178.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLSDIEGTDRDVQSASGTWRSKRIVLAKEGVGFSLHETVLY
                     AGTETTMWYANHIEAVFCVEGEAELTNEETGEKHWISPGTMYLLDGHEKHTIRPKSDFR
                     VMCVFNPPVTGREDHDENGVYPLLTEA"
     gene            complement(5433..6695)
                     /gene="ectB"
                     /locus_tag="CAG99_RS23515"
                     /old_locus_tag="CAG99_23580"
     CDS             complement(5433..6695)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-363). E-value: 5e-73.
                     Score: 238.0. Matches aSDomain:
                     nrpspksdomains_CAG99_RS23515_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433892.1"
                     /locus_tag="CAG99_RS23515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CAG99_23580"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_086161250.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.6e-75, bitscore:
                     251.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFTTLESEVRSYCRAWPTIFDRAQGSYLYDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALLDYLERDGITHGLDMSTTAKRAFLESFQNVILRPRD
                     LDYKVMFPGPTGTNAVESALKLARKVKGRESIISFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDDYLDGRTPDFIWFERLLEDTGSGLNKPAATIVETVQGEGGINVARP
                     EWLRGLADLCRRHDMLLIVDDIQMGCGRTGPFFSFEEAGIVPDIVTVSKSISGYGLPMA
                     LTLFKPELDIWGPGEHNGTFRGNNPAFVTAAAALETYWNDGQMEKQTLGRGEQVESALR
                     AIAAEHSGASYRGRGMVWGLTFEDTARATAVCRRAFELGLLLETSGPQSEVVKLLPALT
                     TTPEELDEGLRVLARAVRETD"
     aSDomain        complement(5607..6608)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CAG99_RS23515_Aminotran_3.1"
                     /evalue="5.00E-73"
                     /label="CAG99_RS23515_Aminotran_3.1"
                     /locus_tag="CAG99_RS23515"
                     /protein_end="363"
                     /protein_start="29"
                     /score="238.0"
                     /tool="antismash"
                     /translation="DRAQGSYLYDEDGHTYLDFFAGAGSLNYGHNNPVLKRALLDYLER
                     DGITHGLDMSTTAKRAFLESFQNVILRPRDLDYKVMFPGPTGTNAVESALKLARKVKGR
                     ESIISFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDDYLDGRTPDFIWFERL
                     LEDTGSGLNKPAATIVETVQGEGGINVARPEWLRGLADLCRRHDMLLIVDDIQMGCGRT
                     GPFFSFEEAGIVPDIVTVSKSISGYGLPMALTLFKPELDIWGPGEHNGTFRGNNPAFVT
                     AAAALETYWNDGQMEKQTLGRGEQVESALRAIAAEHSGASYRGRGMVWGLTFE"
     gene            complement(6762..7343)
                     /gene="ectA"
                     /locus_tag="CAG99_RS23520"
                     /old_locus_tag="CAG99_23585"
     CDS             complement(6762..7343)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969939.1"
                     /locus_tag="CAG99_RS23520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CAG99_23585"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_086161251.1"
                     /transl_table=11
                     /translation="MTPAQADHARARPDFMDREFTAGTDGLTLDTPRLDDGADLWRLAR
                     DSRVLDLNSSYSYLLWCRDYARTSIVARDAAGVPVGFITGYTRPQAPHTLLVWQVAVDG
                     GQRGRGLAAAMLDALARRTGRELGVNMIETTVTPDNTASNRLFASFAARHDAAVEREVL
                     FDAGVFPERGHQPEVLYRIGPIAEPAATAR"
     misc_feature    complement(7500..7515)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7510..7525
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7510..7527)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.31"
                     /tool="antismash"
     misc_feature    7517..7530
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.73"
                     /tool="antismash"
     gene            7848..8972
                     /locus_tag="CAG99_RS23525"
                     /old_locus_tag="CAG99_23590"
     CDS             7848..8972
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560635.1"
                     /locus_tag="CAG99_RS23525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CAG99_23590"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_086161252.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.2e-17, bitscore:
                     62.5, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAGPPSAPVLHVRGRVLAAEGPEGGPDLEIRDELWVVGGRITYD
                     RPPTARDVVRLTGWVLPGLVDAHCHVGLDAHGAVDRATAEAQALADRDAGALLLRDAGS
                     PSDTRWTDARADLPPIIRAGRHIARTRRYIREYAHEIEPDRLAAVVRQEARRGDGWVKL
                     VGDWIDRERGDLAPCWPADALVAAVAAAHEEGARVTAHCFAEDSLADLVAAGIDCVEHA
                     TGLTEDTIPLFAAHGVAIVPTLVNIATFPGLAESGEAKYPRWSAHMRRLHARRYDTVRA
                     AHDAGIAVFTGTDAGGSLPHGLIAREVAELRRAGLPVHDALAAATWRAREWLGRPGLTE
                     GAPADLVVYADDPRRDLAVLAEPRRIVLRGRVVA"
     gene            9079..10011
                     /locus_tag="CAG99_RS23530"
                     /old_locus_tag="CAG99_23595"
     CDS             9079..10011
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF041527.1"
                     /locus_tag="CAG99_RS23530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CAG99_23595"
                     /product="SCO1860 family LAETG-anchored protein"
                     /protein_id="WP_086161253.1"
                     /transl_table=11
                     /translation="MNRTARRGATALLAATVLTTPATARADEAGDRSAGTASAAVLRAG
                     LDVSLLDGGVRLPLTASLNEVSAPDGGGSADDTLLTATLEGVEDGQPFDLLRADVASAS
                     ATTGADGAFAETSLAGARVHLPGLPLLSLIEVDAVSARASCPVGGEASAETVMPATATV
                     LGRTAELTTEGTTEVSVPGVGDVTLALAQRESGDGTASASALDLNVAVDPLDLGVAEVT
                     GTVTLAEAACTAPAPPGPRPQTGIENQPGERPEEGVGRPGDRPAEDVDLAETGGGSSTP
                     YLVGGAAGLLAAGAATVLIARRRRAAAQG"
ORIGIN
        1 gcgacggcga ccacggcggc tgcgagggct gcggcgacgg tcggcctggc cttgcgcacg
       61 atgttctccc tggctcggtg ggggaaggcg aacggaccct agggaacggt tgtttccggc
      121 ctgcccggcc cgggagtcgc gcggacgacg tccgtgtgaa cagccgtggg ggagcggacc
      181 accatccgcg tgcccgcgca ccacgggcgc cgaggcaccc acgcgcgccg caccgagcgg
      241 tgcggtccgg gccgtcggcg gacgtgccgc ggtcaggcgg actccctgcg gaccagttcg
      301 gtgggcagga tcaccgccga ggtgtcctcg cccgccatcc gggcgagcag gacgcggacc
      361 atctcggagc tgatgcggtc ccacggctgc ctgaccgtgg tcaggccggg ccgggaggcc
      421 accgcggccg tcgagtcgtc gaagccgccg acggccacgt cgtgcggcac gcggcggccg
      481 gcgcgttcca gcgcggcgag ggcgccgcgc gccatcaggt ccgaggcgac gaacagcgcg
      541 tcgaggtccg ggcggcgcgc gagcagccgg gccgtggccg ccgcggcgcc gtcgtggctg
      601 tagtcgccgg tctcgaccag gtcctcgtcc gccggcagac cggcctcggc gagcacctcg
      661 cggtagccgg ccaggcgttc cacgccgccc ggggtgtcga gcggcccggt caccgtcgcc
      721 acgcgccgcc ggcccgtgtg cagcaggtgg cggaccatct gccgggcgcc gccccggtcg
      781 tcggccgcca cgtgcgccag gcccgtggcg cgccccacgg gccggccgca catgaccacc
      841 ggcaggccgg cccgcacgag cgtctcggcc atcgggtccc cggtgcgcga ggagacgagc
      901 agcacgccgt cgacgtggcc cgccgagacg tagcgggtga tgcggcgctg ctccgcgccg
      961 ctgcccgcga tcatcagcag cagcgggatg tcgtgcgcgg ccagcgcctg cgtgcaaccg
     1021 cgcaggagca cgcccaggtt cgggtcctcg aagaacctct cctgcggctc ggtcagcagg
     1081 aacgccacgg agccggagcg gccggtgatg aggctgcggg cgtgccggtt gacgacgtag
     1141 ccggtcctgc ggatcgccgc gttcacggcc tcggcagcgg ccgggctgac gtggcggccg
     1201 ccgttgagca cccgggagac ggtgccgcgg gagacgcccg cctcccgcgc cacgtcgtga
     1261 atcgtcggcc gcctgcgccg ggtcgcgttg ttcgtcgggc tcacgggcac gactgtacgg
     1321 cgccggtgcg gggccggggc aagggctggg atcgtgcaca ggaaggggcc ccgtccaacc
     1381 cttgtcaatg gtcggtgctg tgcggttatg ttgcctgtgc acgttcacag gaaaggcagc
     1441 tcgacagatg cgcatggatc gactcgcgtt cggcggggac tacaaccccg agcagtggcc
     1501 ggaagagctg tggcccgagg atgtcgcgct gatgcgcgaa gccggggtga cgctcgtcag
     1561 catcgggatc ttctcctggg cccggctcga accccgtgag ggcgcgtacg acttcggctg
     1621 gctcgaccgg atcatcgacc tgctgcacga gggcggcatc cggatcgacc tcgccacccc
     1681 cacggtcgtc cccccggcgt ggttctaccg cgcgcacccc gaggcgctgc cggtcacgcg
     1741 ggagggcgtg cgcctggagt tcggctcgcg cggggcgatc tgccacagct cgcccgccta
     1801 ccgcgaggcc gccgcgcgca tcacccgcgc gctcggcgag cggtacggcg accacccggc
     1861 gctcgcgctg tggcacgtgc acaacgagta cggagtgccc gtgcttgagt gctactgcga
     1921 cgtctccgcc gcccacttca ggcgctggct cgccgagcgg tacggcaccc tcgacgcgct
     1981 caacgcggcc tggggcacgg ccttctgggg ccaggcgtac ggcgagtggg acgaggtgca
     2041 gccgcccagg ctcaccccca ccacggccaa ccccgcgcag cgcctggact tcgcgcgctt
     2101 cgcctccgac agcgcgctgg agaacttccg cgccgaacgc gacctgctgc acgaactgtc
     2161 ccccggcgtg ccggtcacca ccaacttcat ggtctcgccc acccagtgcg acaccatcga
     2221 ctactggaag tggggccgcg aggtcgacat cgtcaccaac gatcactacc tggtggccga
     2281 ggacgacagg aaccacatca acctctcgtt cgccgccgac ctcacccgtt ccgtcggtgc
     2341 cggccgcccc tggctcctgc tcgaacactc caccagcgct gtcaactggc agccgcgcgg
     2401 catcgccaag cggccgggcg agatggcgcg caacagcctc gcgcacgtcg cgcgcggctc
     2461 gcagggcgcg atgttcttcc agtggcgcgc ctcacgcgcc ggcgcggaga agttccactc
     2521 ggcgatggtg ccgcacgcgg gcaccgacag ccgcgtgtgg cgcgaggtcg tcgaactcgg
     2581 cgggcacatc gacgcgttgg ccgacatcat ggacacccgc acggtggcgg acgcggccgt
     2641 cgtgtgggac tgggagtcct ggtgggcgca caagctggag tggcggccga gcgacgacct
     2701 gggcaccagg gaacgcgcgg aggcgtggta cgccgcgctg ttcgaccagc acctgaccgt
     2761 cgacttcgcg caccccgagt ccggtgacct cgccggccgg ccgctggtcg tcgtcccggc
     2821 cctctacctc gccacgcggg ccgcgggcga gaacctgcgc cgctacgtcg agggcggcgg
     2881 cacgctggtg gtgtcctgct tctccggcat cgtcgacgag cacgacgccg tgcacccggg
     2941 accgcacccc ggcgcgctgc gcgacgtcct cggcctggtc gtcgaggagt tccagccgct
     3001 gcgcgcgggt gagacggtac ggctggcagg tcctgaaggc gccaccgcga ccgccgacgt
     3061 ctgggcggag accgtccgca ccacgggcgc ggaaacggtg tggacctacg ccgatggacc
     3121 cgcggccggc gggcccgcga tcacccgcca ccggctcggt gacggcaccg cctggtacgt
     3181 ctcggcccgc gtgggccagg aggggctgcg tcaggtcctg gccgccgcgg ggaaggacgc
     3241 cggtctcgac ccgcgcgacg acctgccccg cgacgtcgag gtggtcaggc gcgcaggccc
     3301 cggcggcgac ttcctgttcg cgatcaacca cacggccgag gaccacgagg tggccctgcc
     3361 tgcctccggt accgaactgc tcaccggcac ccccgtcacc ggcccgctcg ccgtgcccgc
     3421 gggcgccacc cgggtggtgc gcctggaccg gtgacgccac cggaggcggg aacgccgccc
     3481 gccgaccgct cgacttcgcg ccgggccgct cgccggcccg cgcgcgggtc agctcccggc
     3541 gaggcgttcc agccgcccga ccacttcctc gatgggcgcg gtggcgtcga tctcggcgct
     3601 cgcggtggca cgcagcagcg gctcgaccgt ggccagatga cgcagcacgg cggcccgttc
     3661 ctccggccgc ttgccgtagg ggttgccggt gcgccgctcg atcctggcca gcagtacgtg
     3721 ggccggtgcg ctgagcagca cgacgtggtc gagcagcggg tagaagtcgc cctggttgct
     3781 cttgcagccg gcgaggaaga ggtggcccgc ggcgtggccg gtcagcaggc cggtgatctc
     3841 ccgctcgcgc cagatccagt cggaggaacc gtccggcagg gtcacccagc ggctccaggc
     3901 gtcgctgtcg gtgtccaccg tcctgtgccc ccgcgcgcgc agcaggcgca gcgccgccga
     3961 cttgccggtg cccgacattc cggtgagcag gaccttggcc acggaggcga gcgtacgcga
     4021 acgcggcggg cgccggccct ccccgtggga ggaccggcgc ccgccggccg gcgagtcggc
     4081 tcagctcacc ggcgtgaagt ccctggcccc gatgaactcg ggacggcgga ccggtgccgc
     4141 gaagggctcg accgcggcgt tctccacgct gttgaacacg atgaacacgt tgctgcgcgg
     4201 gtagggcgtg atgttgtcgc ccgagccgtg catgcagttg cagtcgaacc acgtggccga
     4261 gccggcccgg ccggtgaaca gcttgatccc gtgctggtcc gcgaacttgg tcagtgcctc
     4321 gtccgacggc gtgcccgcgt cctgcatctg gagcgacttc ttgtagttgt ccttcggcgt
     4381 ctcacccgcg cagccgagga acgacaggtg cgagcccggc atgatcatca agccgccgtt
     4441 ggtgtcgaag ttctccgtca gcgcgatcga cacggacacc gtccgcatgc ggggcagccc
     4501 gtcctcggcg tgccacgtct cgaagtccga gtgccagtag aacccgctgg cgccgaaccc
     4561 cggcttgacg ttgatccggc tctgatgcac gtacacctcg gagcccagga tctgccgcgc
     4621 ccggtcgagc agccgcgggt cgcgcaccag tccggcgaac ttctcgctga tcttgtgcac
     4681 ctcgaacacc gacctgatct cctgcgacga gggctcgacg atcgagcgct cgtccgcgcg
     4741 gatgtccggg tcggtgacca gccggtccag ttcggcgcgg taggccagca cctcctccgg
     4801 cgtgatcagc tggtcgacgg cggcgaagcc gtcccgttcg aagccggcca gttcgtccgt
     4861 gctgaacggc cccggcgctc cgggctctga ccacaccacc gggtcctggc gtggcgtggt
     4921 gacctcggtc gccccgcggg tcgggtacag gtcggcggtg cgttcgggtg cggtggtcat
     4981 gtgggtgttc cttcctctcc ttacgcctcg gtgagcagcg ggtagacacc gttctcgtca
     5041 tggtcctcac ggccggtgac cggcgggttg aagacgcaca tgacgcggaa gtcgctcttc
     5101 gggcgaatgg tgtgcttctc gtgcccgtcg agcagataca tcgttcccgg cgagatccag
     5161 tgcttctcgc cggtctcctc attggtcagc tcggcctcgc cctcgacaca gaagacggcc
     5221 tcgatgtggt tcgcatacca catggtggtc tcggtacccg cgtagagcac ggtctcgtgc
     5281 agtgagaatc cgacgccctc cttggccagg acgatgcgct tgctgcgcca cgtgccggag
     5341 gcggactgca cgtcccggtc ggttccctcg atgtcgctga gcgatcggac gatcacggcg
     5401 gtgtacttcc cttcccgttc ccagtggttg cttcagtcgg tctcgcggac ggcacgggcc
     5461 agcacccgca ggccctcgtc cagctcctcc ggcgtggtcg tcagcgccgg cagcagcttg
     5521 acgacctcgc tctgcgggcc cgacgtctcc agcagcaggc cgagttcgaa ggcgcggcgg
     5581 cacacggcgg tggcgcgcgc ggtgtcctcg aacgtcaggc cccacaccat gccgcggcca
     5641 cggtaggagg caccggagtg ctccgcggcg atcgcgcgca gcgcggactc cacctgctcg
     5701 ccgcggccga gggtctgctt ctccatctgc ccgtcgttcc agtacgtctc cagcgcggcg
     5761 gcggcggtga cgaaggccgg gttgttgccg cggaaggtgc cgttgtgctc gccggggccc
     5821 cagatgtcga gttccggctt gaacagcgtg agggccatcg gcagcccgta gccgctgatc
     5881 gacttcgaca ccgtgacgat gtcgggcacg atgcccgctt cctcgaacga gaagaacggc
     5941 ccggtgcgcc cgcagcccat ctggatgtcg tcgacgatca gcagcatgtc gtgccgccgg
     6001 cacaggtcgg ccaggccgcg cagccactcg gggcgcgcca cgttgatgcc gccctcgccc
     6061 tgcaccgtct ccacgatcgt ggccgcgggc ttgttcagcc ccgacccggt gtcctcaagc
     6121 agccgctcga accagatgaa gtccggggtg cggccgtcga ggtagtcgtc gaacggcatc
     6181 ggggtgccgt gcaccagcgg gatgccggcg cccgcgcgct tgaacgcgtt gcccgtcacg
     6241 gcgagcgagc cgagcgacat gccgtggaac gcgttggtga aggagatgat cgactcgcgg
     6301 cccttgacct tgcgggccag cttcagcgcg gactccaccg cgttggtgcc ggtcgggccg
     6361 gggaacatca ccttgtagtc gaggtcgcgc gggcgcagga tcacgttctg gaacgattcg
     6421 aggaacgccc gtttcgcggt ggtggacatg tccaggccgt gagtgatgcc gtcgcgctcc
     6481 aggtagtcga gcagggcgcg tttgagcacc gggttgttgt ggccgtagtt gagcgagccg
     6541 gcgccggcga agaagtccag gtaggtgtgg ccgtcctcgt cgtacaggta gctgccctgc
     6601 gcgcggtcga agatggtggg ccaggcgcgg cagtagctgc ggacctcgga ttccagggtg
     6661 gtgaacacgc tcaggtcggg ctgggtgatg gtcacaacgg tctcctgatc tcagggggtg
     6721 atcacgtgga ggggtcggaa gtggtcgcgg tcgggtcgga gtcagcgggc ggtcgccgcc
     6781 ggttcggcta tggggccgat ccggtacagc acctccggct ggtgcccccg ttccgggaac
     6841 acccccgcgt cgaacagcac ctcgcgctcc acggcggcgt cgtgccgcgc ggcgaaggat
     6901 gcgaagaggc ggttggacgc ggtgttgtcc ggtgtgacgg tggtttcgat catgttcacc
     6961 cccagctccc ggcccgtccg gcgggccagg gcgtcaagca tcgcggcggc gaggccgcgt
     7021 ccgcgctgcc cgccgtcgac ggcgacctgc cacaccagca gcgtgtgcgg cgcctgcggg
     7081 cgggtgtacc cggtgatgaa gccgaccggg acgccggccg cgtccctggc cacgatcgag
     7141 gtgcgcgcgt agtcgcggca ccacaggagg tagctgtacg acgagttcag gtccaggacc
     7201 ctggagtccc gggcgaggcg ccacaggtcc gccccgtcgt ccaggcgcgg ggtgtcgagc
     7261 gtgaggccat cggttcctgc ggtgaattca cggtccatga aatccggtcg ggcacgtgca
     7321 tggtctgctt gtgcgggggt catgcagatt caatttaccc agcgattctt gaaattgcac
     7381 cgccggctgg tcttaacagg tcgggcccga tgtgttatcg cgcgcgcggg ggaccgcacc
     7441 ttacttcgga gcaaaaagtt acgttctgtc cgttagttac cgggcaaatc ggtcatcggt
     7501 gtgggcctgg tcacatgttt gtaacgaaga agtggtggtg ctgacccgtt ccggtgaccg
     7561 gccacgatcg tgtatcggcc ccgtttagaa gtcgataaag cgggcagaag aggcccgttg
     7621 ggtccgtctg aattacctgg ggaatcgcag tttgaattcc atcgtaaaac gccgggcgcg
     7681 gcgccgggaa ttatcgcgcg gcggccgggg gtccgccacc cgcggacccg gcgccctgaa
     7741 cccctcgcgc ccgggaacgg gcgcgaggcg gcgcgcgggt ccggcggcag ccgaggtggg
     7801 gcaccggccc tcggcgcggg cggcggcccg gccgtaggct gcggcgcatg aacgcaggcc
     7861 cgccctccgc gcccgtcctc cacgtccggg gccgcgtgct cgcggccgag gggcccgaag
     7921 gcgggcccga cctggagatc cgcgacgaac tctgggtcgt cggcggccgg atcacctacg
     7981 accggccgcc caccgcgcgg gacgtcgtcc gcctcaccgg ctgggtgctg cccgggctcg
     8041 tcgacgccca ctgccacgtg ggcctcgacg cgcacggggc cgtcgaccgc gccaccgcgg
     8101 aggcgcaggc cctcgcggac cgcgacgcgg gcgccctgct gctgcgcgac gccggatcgc
     8161 cctccgacac ccgctggacg gacgcgcgcg ccgacctgcc cccgatcatc cgcgccggcc
     8221 ggcacatcgc ccgcacccgg cgctacatcc gcgagtacgc gcacgagatc gagcccgacc
     8281 ggctcgccgc ggtggtcagg caggaggcca ggcgcggcga cggctgggtc aagctcgtcg
     8341 gggactggat cgaccgcgaa cgcggcgacc tcgcgccctg ctggccggcc gacgcgctcg
     8401 tggccgccgt cgccgccgcg cacgaggagg gcgcacgggt caccgcgcac tgcttcgccg
     8461 aggactcgct cgcggacctc gtggccgccg gcatcgactg cgtggagcac gcgaccggac
     8521 tcaccgagga caccatcccc ctgttcgccg cgcacggcgt ggccatcgtt cccaccctgg
     8581 tcaacatcgc gaccttcccc ggcctcgccg agagcgggga ggccaagtac ccgcgctggt
     8641 cggcccacat gcgccggctg cacgcgcgca ggtacgacac cgtgcgggcc gcccacgacg
     8701 cgggcatcgc cgtcttcacc ggaacggacg ccggaggctc gctcccgcac ggcctcatcg
     8761 cgcgggaggt cgcggaactc cgccgggccg gactgcccgt ccacgacgcg ctggccgcgg
     8821 cgacctggcg ggccagggaa tggctcggcc gcccgggcct gaccgagggc gcgcccgccg
     8881 acctggtcgt ctacgccgac gacccgcggc gcgacctcgc cgtactcgcc gaaccgaggc
     8941 gcatcgtgct gcgggggcgc gtcgtcgcct gagttacccc gttcgggtga acgggtgacg
     9001 taacccggac ggccggcggg agcggaccga aggatggcgg gcgtcccagc aacacccatc
     9061 ccgatggagt cccctgtcgt gaacagaacc gcccgccgcg gcgccaccgc gctgctcgcc
     9121 gccaccgtcc tgacgacccc ggccacggcc cgcgccgacg aggccgggga ccggagcgcg
     9181 gggacggcga gtgccgcagt cctgcgagcc ggcctcgacg tctccctcct cgacggcggc
     9241 gtcaggctcc cgctcacggc gtcgctcaac gaggtcagcg cgcccgacgg gggcggcagc
     9301 gccgacgaca ccctgctgac cgcgacgctg gagggcgtcg aggacgggca gccgttcgac
     9361 ctgctgcgcg ccgatgtcgc ctccgcctca gcgacgaccg gcgcggacgg cgcgttcgcc
     9421 gagacgagcc tcgccggagc ccgcgtccac cttccggggc tgccgctgct ctccctgatc
     9481 gaggtggacg cggtctccgc gcgcgcctcg tgcccggtgg gcggggaggc gtcggccgag
     9541 accgtgatgc ccgccacggc caccgtgctc ggccgcaccg ccgagctgac caccgagggc
     9601 accaccgagg tgtcggtgcc gggcgtcggc gacgtcaccc tcgcgctcgc ccagcgcgag
     9661 tcgggcgacg gcaccgcgag cgcgagcgcg ctcgacctga acgtggcggt cgacccgctc
     9721 gacctgggcg tcgccgaggt caccggcacc gtcacgctcg ccgaggccgc ctgcaccgcg
     9781 cccgccccgc ccggcccgcg cccccagacc ggcatcgaga accagccggg cgagcggccc
     9841 gaggagggcg tcggccggcc gggcgaccgg ccggcggagg acgtcgacct ggcggagacc
     9901 ggcggcggct cgtccacgcc ctacctggtc ggtggagcgg ccggcctgct cgccgccggc
     9961 gcggccaccg tgctgatcgc ccgccgccgc agggccgccg cgcagggctg accggcccgg
    10021 cgcacggcgc cgcgggaacg cgggccgggc gcgcgcgagg cgcgcgcccg gcccgtcccc
    10081 tgtcgccgcg cgccccgcct gccggacaat cacccgtggc cggcggcgcc gatgacgagc
    10141 ggtgcggccg atgacgagcg gtacagggga gacgggtgga cgcggcgatg ggcgaacgag
    10201 ggccgggcga gacgtccggc gggtggccgg acgcgtggca ggaggcgtgg cacgacctgg
    10261 tcgcgcacgc gcggcggacg gtggcggacg gcctcgtggt cggcacctcc ggcaatgtgt
    10321 ccgtccgcgt gggcgacgcc gtgctcgtca ccccctcagg catcccctac gaacggctgc
    10381 gcgcccgcga cctgct
//
