LOCUS       NZ_CP021121            10417 bp    DNA     linear   CON 31-JUL-2024
DEFINITION  Streptomyces marincola strain SCSIO 03032 chromosome, complete
            genome.
ACCESSION   NZ_CP021121
VERSION     NZ_CP021121.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces marincola
  ORGANISM  Streptomyces marincola
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6287975)
  AUTHORS   Ma,L., Zhu,Y., Zhang,W., Zhang,G., Tian,X., Zhang,S. and Zhang,C.
  TITLE     Complete genome sequence of Streptomyces sp. SCSIO 03032 revealed
            the diverse biosynthetic pathways for its bioactive secondary
            metabolites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6287975)
  AUTHORS   Ma,L., Zhu,Y., Zhang,W., Zhang,G., Tian,X., Zhang,S. and Zhang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2017) Key Laboratory of Tropical Marine
            Bio-resources and Ecology, South China Sea Institute of Oceanology,
            164 West Xingang Rd., Guangzhou, Guangdong 510301, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2016
            Assembly Method :: Hierarchical Genome Assembly Process v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 165.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002128305.1-RS_2024_07_31
            Annotation Date                   :: 07/31/2024 06:55:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,513
            CDSs (total)                      :: 5,438
            Genes (coding)                    :: 5,343
            CDSs (with protein)               :: 5,343
            Genes (RNA)                       :: 75
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 19 of 95
            Pseudo Genes (incomplete)         :: 88 of 95
            Pseudo Genes (internal stop)      :: 2 of 95
            Pseudo Genes (multiple problems)  :: 13 of 95
            CRISPR Arrays                     :: 12
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:29:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4759339
            Orig. end                         :: 4769756
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP021121.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Key Laboratory of Tropical
            Marine Bio-resources and Ecology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10417
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="22"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10417
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5417](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5417)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    103..118
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.0"
                     /tool="antismash"
     gene            complement(532..1362)
                     /locus_tag="CAG99_RS20770"
                     /old_locus_tag="CAG99_20775"
     CDS             complement(532..1362)
                     /GO_function="GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455580.1"
                     /locus_tag="CAG99_RS20770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CAG99_20775"
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="WP_086160790.1"
                     /transl_table=11
                     /translation="MTDNTSFARRLRLRRLHHHDPERLFIVPLDHSVTDGPLTPAVRLD
                     ALVGQLAAHHVDAVVLHKGALRHVDTEWFASTSLIVHLSASTVHAPDPDAKYLVASVEE
                     SLRAGADAVSVHVNLGSREERRQIADFAAVSEACDRWNVPLLAMVYPRGPAIENPRSPA
                     LVAHAVQLAADLGADLVKTPYAGSVTDMAAITAAAPIPVVVVGGPRDHDEARTLAYVSD
                     ALRGGAAGVAMGRNVFQSPDPGAMADKLSGLIHRKATHLGRAVRAGRPGQAVPV"
     gene            complement(1377..2660)
                     /locus_tag="CAG99_RS20775"
                     /old_locus_tag="CAG99_20780"
     CDS             complement(1377..2660)
                     /EC_number="2.7.2.4"
                     /GO_function="GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380463.1"
                     /locus_tag="CAG99_RS20775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CAG99_20780"
                     /product="aspartate kinase"
                     /protein_id="WP_086160791.1"
                     /transl_table=11
                     /translation="MRTLGARGAGEVVVQKYGGSSLATGHQLEAVARRVADAARGGRPV
                     VTVVSARGDTTDELIDLARGINPAPRGRELDQLLATGETASAALLAMALQHIGQPATAL
                     TGAQSGILVNGPHGLGTIVAIDTERATRLLGKGEVVVVAGFQGITASGDTITLGRGGSD
                     TTAVALAAELGSRSCEIYTDVPGVLTADPRVVPNARLLPEVDIDVMAEMAFAGARVMHA
                     RAVELAALYGIDILVGGSTATHAGTRIHHRDGQDMLEARAAVVAVVHEADVTRVTFRSA
                     AGPEDPARAVFGSLADAAIPADMATVTSTPDGDVAVGLTVGGAHTSAVRACLAPLAGPA
                     GGVEVDEHLAKVSIVGKGLLSRPEYVSRMLNSLGADGVGARLLAASQLRVSVTVPSGES
                     GRAVRVLHSEFGLDAGTEAAHRHLSHQP"
     misc_feature    complement(2698..2711)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.3"
                     /tool="antismash"
     gene            complement(2996..3826)
                     /locus_tag="CAG99_RS20780"
                     /old_locus_tag="CAG99_20785"
     CDS             complement(2996..3826)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382991.1"
                     /locus_tag="CAG99_RS20780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CAG99_20785"
                     /product="creatininase family protein"
                     /protein_id="WP_086160792.1"
                     /transl_table=11
                     /translation="MNSGAGDDILRGTLAELTWPEVARAAREGAVLLWAFGVIEQHGPH
                     LPTGTDVYLPSAWLRAVRRHLADLGVPALIVPPYYWGVNVVSAGFPASWSVRPAVMTEL
                     MADVFSGLASDGFRHVFCFSGHGDALHNRTVHAGVLIGRARYGIDATFVAEPDLVARLP
                     LDPADPAVTVLPAPPPPAAAFPDLHAGRAETSMMLADRPGLVRPGHAGLPPVVLGPDEV
                     ARWRRGHRHARRITPDGHVGDPAAGDAAEGAAATEDAARRAAHAVAARLRRADR"
     gene            complement(3823..4041)
                     /locus_tag="CAG99_RS29005"
                     /old_locus_tag="CAG99_20790"
     CDS             complement(3823..4041)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CAG99_RS29005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CAG99_20790"
                     /product="hypothetical protein"
                     /protein_id="WP_086160793.1"
                     /transl_table=11
                     /translation="MVPLRQLTAAAAGRDGSPGPSGPRPAGGRGTGRPPREQLATRALP
                     RFAELRAEVFGLVKQAKTYHDAPGART"
     gene            complement(4020..4940)
                     /gene="griF"
                     /locus_tag="CAG99_RS20790"
                     /old_locus_tag="CAG99_20795"
     CDS             complement(4020..4940)
                     /EC_number="1.10.3.15"
                     /codon_start=1
                     /gene="griF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515805.1"
                     /locus_tag="CAG99_RS20790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CAG99_20795"
                     /product="grixazone synthase"
                     /protein_id="WP_086160794.1"
                     /transl_table=11
                     /translation="MVHIRKNHLDMTREEKRRFVHAVREIKRRGIYDRFVDLHIRINSR
                     DYLDKETGKRLGHINPGFLPWHRQYLMRFEQELQRVDPRVTLPYWDWTVDHGEDSPLWD
                     EEFMGGNGRPGDRRVMTGPFARDNGWVLNISVVPVGEENPALNGNYTHDDRDFLVRDMA
                     TLTDSLPSPQELEDTLALPVYDCPPWNHTSGGEAPYESFRNHLEGYTKFPWETKFGKLH
                     GAGHLWVGGHMMYIGSPNDPVFFLNHCMIDRCWALWQQRHPDVPHYLPLHDTPDVPDLH
                     TPLGPWHTMTPADLIDHTRWYRYDS"
     gene            complement(5001..5417)
                     /locus_tag="CAG99_RS20795"
                     /old_locus_tag="CAG99_20800"
     CDS             complement(5001..5417)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380458.1"
                     /locus_tag="CAG99_RS20795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CAG99_20800"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_086162382.1"
                     /sec_met_domain="melC (E-value: 1.1e-29, bitscore: 101.6,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLNRREMVAGVAGTALVAAAAATPLVTGSGGGADAHAAHGLAGG
                     EGGEGDGDAAEYVETYQGRTIRVLPEAAGGGVLIDDRPLHLMKFGDDAYLSSMCHYEMA
                     PSPLHAARRAVEELRGAALLPAAHGSHGTHVMVV"
     gene            complement(5906..6154)
                     /locus_tag="CAG99_RS20800"
                     /old_locus_tag="CAG99_20805"
     CDS             complement(5906..6154)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CAG99_RS20800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CAG99_20805"
                     /product="hypothetical protein"
                     /protein_id="WP_086160795.1"
                     /transl_table=11
                     /translation="MAGTSITGAAGNAAITCPRVDADSVVESLAGDGRITRTGPVDGGG
                     RVNGDDPLTVAAHNGVVDGALGIDPCMGASGTPAVNR"
     gene            complement(6576..7946)
                     /locus_tag="CAG99_RS20805"
                     /old_locus_tag="CAG99_20810"
     CDS             complement(6576..7946)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515809.1"
                     /locus_tag="CAG99_RS20805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CAG99_20810"
                     /product="MFS transporter"
                     /protein_id="WP_255308246.1"
                     /transl_table=11
                     /translation="MSTRETLPPTAAAPPGARPISPRSAGLRYGALFGPAVFGVTAAGV
                     ALPDVASALDATPSAVAWVLTAHALALGVGTALFGRLADSRGLRTSLLLGSLVLAVGAA
                     VCLFAPNLPVLVAGRFVLAAGSGAMTSVAVTLSASVAPADRPKVLAGFGATMAVFSASA
                     TLAGGVFTEWVTWRVALILPALSLLAVPLCLRPAAARAASGRPLDLPGAVLLTVAASSF
                     LLLIQASALDLSTALVVGLAVVLPASIAVLVVRVRAVPASFVPRWLITDSLFLRAAGTG
                     IGVYAGLFAAMYTVPQILVDEHGLSVLAVGLWLLPGAVVGAVLSRLAGRLPVTSGGPVL
                     IAVIACVMAVTLAVSLGSENAVLPVVGASLGFAAFAATQVVSTGLMGAHVEPALRGGAT
                     GLLNLAFFVGGGVGSATAGALAKSASLYTTLGIVAGFPLIAAALALTLTRRGARADG"
     misc_feature    complement(7939..7954)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.49"
                     /tool="antismash"
     misc_feature    8408..8425
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.59"
                     /tool="antismash"
     misc_feature    9066..9081
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.21"
                     /tool="antismash"
     misc_feature    10225..10236
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
ORIGIN
        1 agggagccct cggcgcgggc cgccgcggcg atcacgatct ccagcgggat cttggtgggg
       61 tcgcggaaca gcagcaggct ccacttctcg gtgcgcgccg actcgcaggc gtcttcgagg
      121 gtgggcgcgt cgatgatctc cacgaaccgg ccgaactcga caccggggtg ccgcgtggcc
      181 agctcggccg gggtcacgcc gtgcgtgcgc gggtcgacga cgacgatcgc ggcgtccccg
      241 aagtcctcgg gcagcggctt gccctgcggg aacagcacct tggtcacggt gggcggcagg
      301 tccgcgaaga gcgcggggtc gtcggcgacc acggcgtcga cgcggtggtg cagcgcctcc
      361 tgaacgatgg cgtcgcgcgc ctcgccgagg gaacggatgt cgagccagct gagcttcacg
      421 ggagaactcc tcgggtcgtt gcttcgggca cgggcgggcc gaacgggcgg aacggaccgg
      481 ccggttctgg ggtgacggcg ggcgggtcgg cggggcgcgg ccatgcggcg ctcacaccgg
      541 gacggcctgc ccggggcgcc cggcccgcac cgcgcggccc aggtgcgtgg ccttccggtg
      601 gatgaggccg gagagcttgt cggccatggc gccgggatcg ggggactgga agacgttgcg
      661 ccccatcgcg accccggccg cgccgccgcg cagggcgtcc gacacgtagg ccagggtcct
      721 cgcctcgtcg tggtcgcgcg ggccgccgac gaccacgacc gggatggggg ccgccgccgt
      781 gatggcggcc atgtcggtca ccgagcccgc gtacggcgtc ttgaccaggt cggcgccgag
      841 gtcggcggcc agttgcacgg cgtgcgcgac gagcgcgggc gaccgcgggt tctcgatcgc
      901 ggggccgcgc gggtagacca tcgcgagcag cggcacgttc caccggtcgc acgcctccga
      961 gacggcggcg aaatccgcga tctgccgccg ctcctcgcgc gagccgaggt tcacgtgcac
     1021 gctgacggcg tcggcaccgg cccgcaggct ctcctcgacg ctcgcgacga ggtacttggc
     1081 gtccgggtcg ggcgcgtgca ccgtgctcgc gctcaggtgc acgatcagcg aggtgctggc
     1141 gaaccattcc gtgtccacgt gccgcagcgc gcccttgtgc aggacgacgg cgtccacgtg
     1201 gtgcgcggcg agctggccga ccagcgcgtc gagccgcacc gccggggtga gcgggccgtc
     1261 ggtgacggag tggtcgagcg gcacgatgaa cagccgctcc gggtcgtggt ggtgcagccg
     1321 ccgcagtcgc agccggcggg cgaaggaggt gttgtccgtc atgaggagtc caggggtcag
     1381 ggctggtggg acaggtggcg gtgtgcggcc tccgtgccgg cgtcgaggcc gaactcggag
     1441 tgcagcaccc gtaccgcgcg gcccgattcg ccgctcggca ccgtgaccga gacccgcagc
     1501 tgcgacgccg cgagcaggcg ggcgccgacg ccgtcggcgc ccagggagtt gagcatgcgc
     1561 gagacgtact ccggcctgct gagcaggccc ttgccgacga tggacacctt ggccaggtgc
     1621 tcgtcgacct ccacgccgcc ggccggcccg gcgagcgggg cgaggcatgc gcgcaccgcg
     1681 ctggtgtgcg ccccgccgac ggtgagaccc accgccacgt cgccgtcagg tgtgctggtg
     1741 acggtggcca tgtcggccgg gatggccgcg tccgcgaggc ttccgaacac cgcgcgggcc
     1801 gggtcctccg ggccggcggc gctgcggaac gtcacccgtg tcacgtccgc ttcgtgtacg
     1861 accgccacga cggcggctct ggcttccagc atgtcctgcc cgtctctgtg atggatgcgc
     1921 gtgcccgcgt gcgtggccgt cgagccgccg acgaggatgt cgatgccgta gagggcggcg
     1981 agttcgacgg cgcgcgcgtg catcacgcgc gccccggcga acgccatctc ggccatgacg
     2041 tcgatgtcga cctccggcag cagccgggcg ttcggcacga cgcgcgggtc ggcggtgagc
     2101 acgcccggga cgtccgtata gatctcgcag ctccgggagc cgagttcggc ggcgagtgcc
     2161 accgcggtgg tgtcggagcc gccccggccc agggtgatgg tgtcgcccga ggccgtgatg
     2221 ccctggaaac cggcgaccac gacgacctcg cccttgccga gcagcctcgt ggcgcgttcg
     2281 gtgtcgatcg ccacgatcgt gccgagcccg tgcggtccgt tgacgaggat gccgctctgc
     2341 gcgccggtca gggccgtggc cggctgcccg atgtgctgga gcgccatcgc gagcaacgcg
     2401 gccgacgcgg tctcgccggt cgccagcagc tggtcgagtt cacggccgcg cggagcggga
     2461 ttgatgcccc gcgcgagatc gatcagctcg tcggtggtgt cgccgcgggc ggacaccacc
     2521 gtgaccaccg gccggccgcc gcgtgccgcg tccgcgacgc gccgtgcgac ggcttcgagc
     2581 tggtggcccg tggccagcga actgccgccg tacttctgca ccaccacctc gcccgcgccc
     2641 cgggctccca gcgtcctcat gccgcctccg ctccctgcac cggctgtccg cattgcctcc
     2701 ctgagcgtga cggaggccgg tatcggcacc gttctcgccg cgcaccgcgg tcgcattggc
     2761 cgcgcatcgc cgggcgttcg cgggtatcgg ggaggcgtcc cgggggcgtc cgcgaggcgt
     2821 ccgggacgcg acccggcccg cgggggcgcg gaagggcggt cccgggggcg cgaaaggcgg
     2881 gggcaagcgc gggaggggcg cgcgacgcgc taccggccgc gcgcatggcg cgggcccagg
     2941 acggccggcg tcctgggccc gggggcggct gggggatcga aaggggcgcg cgggctcagc
     3001 ggtccgcgcg tcgcaggcgc gccgccacgg cgtgcgcggc gcgtctggcg gcgtcctcgg
     3061 tggccgccgc gccctcggcg gcgtcgccgg cggccggatc gccgacatgg ccgtccggag
     3121 tgatccggcg ggcgtgccgg tggccgcgcc gccacctggc cacctcgtcg ggaccgagca
     3181 ccacgggcgg cagcccggcg tgcccgggac gcacgagtcc tggccggtcg gccagcatca
     3241 tcgacgtctc ggcccggccg gcgtgcagat cggggaacgc ggcggcgggc ggcggcggcg
     3301 cgggcagcac ggtcaccgcc gggtcggcgg ggtcgagcgg cagccgcgcc accaggtcgg
     3361 gctcggccac gaaggtcgcg tcgatcccgt accgcgcccg gccgatcagg acaccggcgt
     3421 gcaccgtgcg gttgtgcagc gcgtcgccgt gcccggagaa gcagaacacg tgccggaacc
     3481 cgtcgcttgc gagcccggag aacacgtccg ccatcagctc cgtcatcacc gcgggccgca
     3541 cgctccacga ggcggggaag ccggccgaca cgacgttgac gccccagtag tagggcggca
     3601 cgatcaacgc gggcacgccc aggtccgcca ggtgccgccg caccgcgcgc agccaggcgc
     3661 tcggcaggta gacgtcggtg cccgtcggca ggtgcgggcc gtgctgctcg atcacgccga
     3721 acgcccacag cagcaccgcg ccctcgcggg cggcccgcgc cacctcgggc caggtcagct
     3781 ccgcgagcgt gccgcgcagg atgtcgtcac cggcgccgct gttcacgtcc gcgcgcccgg
     3841 cgcgtcgtgg tacgtcttgg cctgtttgac caggccgaac acctcggccc gcagttcggc
     3901 gaaccgcggc agggcgcgcg tggccaactg ctcgcgcggc gggcggccgg tgccccggcc
     3961 gcccgccggg cgggggccgg acggcccggg ggagccgtcg cggcccgccg cggcggcggt
     4021 cagctgtcgt agcggtacca ccgggtgtgg tcgatcaggt cggcgggcgt catcgtgtgc
     4081 cagggcccga gcggcgtgtg caggtcgggc acgtccgggg tgtcgtgcag cggcaggtag
     4141 tggggcacgt cggggtgccg ctgctgccac agcgcccagc agcggtcgat catgcagtgg
     4201 ttgaggaaga acaccgggtc gttgggcgag ccgatgtaca tcatgtggcc gcccacccac
     4261 aggtgcccgg ccccgtgcag cttgccgaac ttcgtctccc aggggaactt ggtgtacccc
     4321 tccaggtggt tgcggaagct ctcgtagggc gcctcgccgc ccgaggtgtg gttccacggc
     4381 gggcagtcgt agaccgggag cgcgagcgtg tcctcaagct cctgcgggct cggcagcgag
     4441 tccgtcaggg tggccatgtc gcgcaccagg aagtcgcggt cgtcgtgcgt gtagttgccg
     4501 ttgagcgcgg ggttctcctc gccgaccggg accacgctga tgttgagcac ccagccgttg
     4561 tcccgggcga acggccccgt catcacccgc cggtcgcccg gccgcccgtt gccgcccatg
     4621 aactcctcgt cccacagcgg ggagtcctcg ccgtggtcga ccgtccagtc ccagtagggg
     4681 agggtgacgc gcgggtcgac gcgctggagc tcctgctcga agcgcatcag gtactgccgg
     4741 tgccagggca ggaagccggg gttgatgtgg ccgagccgct tccccgtctc cttgtcgagg
     4801 tagtcgcgcg agttgatgcg tatgtgcagg tcgacgaatc tgtcgtagat gccgcgccgc
     4861 ttgatctcgc gtaccgcgtg gacgaagcgg cgcttctcct cgcgggtcat gtccaagtgg
     4921 ttcttgcgga tgtgcaccat ggggggtccc ctcggttcct gtggcggctg ggtccgtgcg
     4981 gctggtgagc ggtggcggcg tcagacgacc atcacgtgcg tgccgtgcga gccgtgcgcg
     5041 gccggcagga gggccgcccc gcgcagctcc tccacggccc ggcgggcggc gtgcagcggg
     5101 gagggcgcca tctcgtagtg gcacatggag ctgaggtacg cgtcgtcgcc gaacttcatc
     5161 aggtgcagcg gccggtcgtc gatgagcacg ccgccgcccg cggcctcggg cagcacccgt
     5221 atggtgcggc cctggtacgt ctcgacgtac tccgcggcgt ccccgtcgcc ctcgccgccc
     5281 tcgccgcccg caaggccgtg cgcggcgtgc gcgtccgccc cgccgcccga acctgtcacc
     5341 agcggggtgg ccgccgcggc ggccaccagt gccgtgccgg cgacgccggc caccatctct
     5401 cgcctgttca aacgcatgcc ggacaaccct tctgacgtgg tggacgcccg ggcgcggcag
     5461 tgcccgggcg gagcgggccg cggcggccgg gccaggccgg cggccacgag gccctggggt
     5521 tctggcagga actgcgtgca gttgtcgtcg tgcgcgccgc gggggccggg gaccggggag
     5581 ccgtggcgta ccgggaacgg cggcccgggg cgggcggcgc ggtcggccgg cagccgcgcg
     5641 gggcgcggcg gcgacgggcc ttcgagcagt gccacgacgc cgttcccggc ccggacgcgg
     5701 cgcttgcgcc catcgtcggg ccgtcggcta tcgccgccgt atcgcggccg tttcggcgca
     5761 cgcggcgtgg acgctccggc gcctccggcg cagttcgcga ccgggggccc ggtgccggtg
     5821 gggcggggga gcggcggggc gcctcgcgca cccgcggcgg aacgcgcggc gggacccgac
     5881 cggcgcgcgt tccgccgcgg gccgttcagc ggttgacggc gggggtgccg ctcgccccca
     5941 tgcacgggtc gatgccgagg gcgccgtcga cgacgccgtt gtgcgcggcg accgtgagcg
     6001 ggtcgtcgcc gttgacccgg ccgccgccgt ccacggggcc ggtgcgggtg atgcggccgt
     6061 cgcccgcaag ggactcgacg acggagtccg cgtccacccg cgggcaggtg atcgcggcgt
     6121 tgcccgcggc gccggtgatc gacgtgcccg ccatggccgt tccgtcgacg acgcggtggg
     6181 cgacggcgtg cgcgggcgtg gcgagggcgg cgggaagcag caggacgagt gccggggcga
     6241 ggacggacga cagggcggtg cgcgtcaggc tgccggggtg gcggggcggg gcgtcgcgat
     6301 tctggcgcgg acggttccgc cggtccggac atgtccggac cggcggggag agggaacgcg
     6361 gacgcctcag ggcgcgcggg cgtgcggaag cgcgccggga cgggcctgcc cggcgcgcgc
     6421 ggcgcggacc ctcgggcggc tcgcgcggcg gtgcgccggc cgcaccgcga catggcgtcg
     6481 cggtgcggcc ggcgcaccga ggtctccggc gccgcccggc gcccccgacg gccggccgcc
     6541 gggggcgcgg gaccgcgccg gagacctcgg gtgtgctacc cgtcggcccg ggcgccgcgc
     6601 cgggtcaggg tcagcgcgag cgcggcggcg atcagcggga agcccgcgac gatgcccagc
     6661 gtggtgtaca gggaggccga cttcgccagg gcgcccgcgg tggcgctgcc cacgccgccg
     6721 ccgacgaaga acgcgaggtt gagcaggccc gtcgccccgc cgcgcagggc gggttcgacg
     6781 tgcgcgccca tcagccccgt ggacaccacc tgcgtggcgg cgaaggccgc gaagccgagc
     6841 gaggcgccga cgacgggcag gacggcgttc tcggaaccga gcgagacggc cagggtcacc
     6901 gccatcacgc aggcgatgac ggcgatcagc accgggccgc cgctcgtcac gggcagccgc
     6961 ccggcgagcc gggagagcac cgcgcccacc acggcgcccg gcagcagcca caggccgacg
     7021 gcgagcacgc tcagcccgtg ctcgtcgacg aggatctgcg gcacggtgta catcgcggcg
     7081 aacagccccg cgtacacacc gatgccggtg cccgccgcgc gcaggaacag gctgtcggtg
     7141 atcagccacc gggggacgaa cgacgcgggc accgcgcgca cccgcacgac cagcacggcg
     7201 atcgaggccg gcaggacgac ggcgagcccc acgacgagcg cggtcgacag gtcgagcgcg
     7261 gacgcctgga tgagcagcag gaacgaggag gccgcgaccg tgagcaggac ggcgcccggc
     7321 aggtcgagcg gccggcccga ggccgcgcgt gccgcggcgg gccgcaggca cagcgggacg
     7381 gcgagcaggg agagcgccgg caggatcagg gcgacccgcc aggtcaccca ctcggtgaac
     7441 acgccgccgg ccagggtcgc gctcgccgag aagaccgcca tggtggcgcc gaacccggcg
     7501 agcactttcg gccggtcggc gggggcgacg gacgcggaga gcgtgacggc gaccgaggtc
     7561 atcgcgccgg agccggcggc caggacgaaa cggccggcca ccagcaccgg gaggttcggc
     7621 gcgaacaggc acaccgccgc gcccaccgcg aggaccagcg agccgagcag gagcgaggtg
     7681 cgcaggccgc gggagtcggc gaggcgcccg aacagggcgg tgccgacccc gagcgcgagg
     7741 gcgtgcgcgg tcagcaccca cgcgacggcc gacggggtgg cgtccagggc actggccacg
     7801 tcgggcaggg cgacgccggc ggcggtgacg ccgaagacgg cgggtccgaa cagcgcgccg
     7861 tagcgcagcc cggccgaccg ggggctgatc ggccgcgcgc cgggcggggc ggcggcggtg
     7921 gggggcaggg tctcacgtgt ggacacgggt aatccttccg ccaccggttg gtcctccggt
     7981 ggctcgtcga cggggtgcgg gggcgtggcg tcgtggtcag ccgtcgcggc ggatggcgtg
     8041 gcccaggcgg aacatgtcac gcgggtcgta ggcggacttg agggccgcga gccgggcgcg
     8101 ggtggcctcg gggaagcact cgcgcgcggc gtcggccgtg tcggggcccg cgaggaagtt
     8161 gaggtacctg aggccggtgc cccagggggc catcccgcgc aggaccaggt cgggggagcc
     8221 gggactggag agcgtggaga gggtgaacgc gccgtcgcgg ctgcccacgg cgttgggcac
     8281 cgcggccggg cgggacaggg cgccgccgag gtggcgcagc tccaccacca ggtcgttccc
     8341 ctcggagtca ggccctgcga gcgccagcag gcccgccacc gcgctctcgt ccaactcccg
     8401 tagcaccatg ttgcgttcgt ggtacggcag cggctcggtc gggtcgcggt gtatgtcggc
     8461 gacggccgtg tagggcatgg gtccgacggc atcgagcagc gcgggcccca cggcgcgcag
     8521 cgggcgcagc agtttctccc cctccgcgtc cgagccggtg tacgcgacgc gcaggtgcgc
     8581 cacgaggcga tcgcgcagaa acgcgggtat ggcgggcagg tcgggcagtc gcagcagcgc
     8641 gaccgacgac gtcatggcct cgggaacgtc ggccgtccag cggcgccagg tgcgcagcag
     8701 cggccccgac aggtcgccgg ggaagtagat gccgccgccg tagagccggg agacgggcat
     8761 gagcccgaac tccatggcgg tgacgacgcc gaggctcccc ttgccgccgc gcagtcccca
     8821 gaacaggtcg ccgtgccgct ccgcggtgac gtccctgagc gctccgtcgg cggtgaccag
     8881 gcgtatgcgg acgacgtggt cggccgcgta gccgtactgg cggcccaggg ggccgaggcc
     8941 gccgcccagc gtgtacccga cgacgcccac caggtgcgac gagccgttga gcggtgcgag
     9001 accgtgcttg gccgcggcgg cgatgacctg gtgccagcgc gccccggcct ccacccgcgc
     9061 ggtgcggtta cgcgcgtcaa cacgcaccgc gttcatgcgc cgggtgctga tcagcacgcc
     9121 gccgtcggcc ggggcggcga tgccgtgccc ggtcgcctgc actccgacgg gcaggtcgag
     9181 cgccgccgcg aaccgcacgg ccgcctgcac gtcggcctcc gtcacggcct gcaccaccac
     9241 gtcgggccgg tgccgggcga tgaggttgaa gccggacagt tcggcctcgt actcggcgtc
     9301 gcccggggtg aagacctcgc cgcgcacctg agtgatcagc cggtcgagcg tctcgcgcga
     9361 cgcggcgggt gcctggggct gctccgccat cgttcgttcc ttcctgccgg ggccggacga
     9421 gccccttgtc acacgcgtcc ggcgctggtc aggagtctgc tgtgggcggg cgccaggacc
     9481 caccccccgt aatcgaggta ggtggccggc cccctccccc tcaaccgagg gggagaggcg
     9541 gaccgggcgc cagggagcgg gcacgagcgc gtggcgcccc gaggcggcga acggcagcgg
     9601 tcttcggccg ccggtgccgt cggcgagccg cgtgtacgcc tcgcgcagga ccgcgatctc
     9661 cgcttcgagc gatgcgatcc ggtcctgggc ggccggggcg ccggcgccgt gcggctgccg
     9721 cgcgggcccg ccctccggcc gctgcccggc gccgtgcccc ggcccggcgc cgccgaccgg
     9781 ccgggacgac aggtcgcagt ccacctcgcc gccgagtatg cgctgttcta tggtgcgcag
     9841 ccgctcgccc ggctcgatgc ccagctcgtc ggagaagcgc cggcgggtct gccggtacag
     9901 cgacagggcc tcggcggtgc ggccggagcg gaacagggcg atcatcagct gcgccgccag
     9961 cgtctcgcgg aacgggttct ccgcgacgtg gtgggtgagt tcccccagta tccgccggtg
    10021 cctgccgagt tgcagttcca gttcgaagcg gtcctcaagg gcggcgtaac ggtcctcgtg
    10081 gaacttcagc gccaccgttt ccagtaacgc actgtcgagc ccggcgccga tcggcccgcg
    10141 ccacaggccc agcgcgtgct ggaaggcgcg caccgtctcc tcactgggac cggcgtcggc
    10201 cgccgcgcgc gccctggcga ccagggacag gaacgcatgc aggtccacct cgctttccgc
    10261 gaccttgatc tcatagccgg acttgtgcgt ctctatggtg gcggcgacgc caccttcggt
    10321 gaattgcctc cgcaacgagg acacgcagat ccgcagctgg ctgcgggcgg tcgcgggcgg
    10381 cgcatcgtcc cagatcgcac gcagcaggcg gtccgcc
//
