LOCUS       NZ_CP019779            11935 bp    DNA     linear   CON 14-MAR-2024
DEFINITION  Streptomyces sp. MOE7 chromosome, complete genome.
ACCESSION   NZ_CP019779
VERSION     NZ_CP019779.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MOE7
  ORGANISM  Streptomyces sp. MOE7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8399509)
  AUTHORS   Elnahas,M.O., De Leon,K.B., Amin,M.A., Hussein,M.M.D., Ali,A.E. and
            Wall,J.D.
  TITLE     Complete Genome Sequencing of Streptomyces sp. Strain MOE7, Which
            Produces an Extracellular Polysaccharide with Antioxidant and
            Antitumor Activities
  JOURNAL   Genome Announc 5 (22), e00442-17 (2017)
   PUBMED   28572320
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399509)
  AUTHORS   Elnahas,M.O., De Leon,K.B., Amin,M.A., Hussein,M.M.D., Ali,A.E. and
            Wall,J.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2017) Biochemistry, University of Missouri
            Columbia, 117 Schweitzer Hall, Columbia, MO 65211, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 8.1
            Genome Coverage                   :: 180.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002090335.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 02:19:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,435
            CDSs (total)                      :: 7,344
            Genes (coding)                    :: 7,005
            CDSs (with protein)               :: 7,005
            Genes (RNA)                       :: 91
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 339
            CDSs (without protein)            :: 339
            Pseudo Genes (ambiguous residues) :: 0 of 339
            Pseudo Genes (frameshifted)       :: 230 of 339
            Pseudo Genes (incomplete)         :: 155 of 339
            Pseudo Genes (internal stop)      :: 7 of 339
            Pseudo Genes (multiple problems)  :: 47 of 339
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:50:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8153160
            Orig. end                         :: 8165095
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019779.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Judy D. Wall 117 Schweitzer
            Hall Columbia, MO USA 65202.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11935
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="29"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11935
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11935
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6935](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6935)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    236..251
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.9"
                     /tool="antismash"
     gene            1024..1953
                     /locus_tag="STRMOE7_RS35775"
                     /old_locus_tag="STRMOE7_35790"
     CDS             1024..1953
                     /GO_function="GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /GO_process="GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981402.1"
                     /locus_tag="STRMOE7_RS35775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35790"
                     /product="aminoglycoside phosphotransferase family protein"
                     /protein_id="WP_084775736.1"
                     /transl_table=11
                     /translation="MHLPTSIDVPEAFAASYGGQGAEARAWLAGLPRLGGDLLERWDLR
                     PDGPAGHGMASLVLPVLRADGTPAALKLQQLREETAGAATGLRTWRGNGIVRLLDHDEE
                     SSSQLLERLDATRTLSSVADDARALRILAELLARLSSVPAPEGLRTLSGLAAAMLDQVP
                     RAVPALRDPAEQRLVRTCASAVAELVGEPGDRLLHWDLHYDNVLAGEREPWLAIDPEPL
                     AGDPGFELLPALHNRWAEVVATGDVERAVLRRFDLLTEALGLDRRRATGWTLGRVLQNA
                     LWDIEDGKTHLEPAQIAIAGALLGRATG"
     gene            complement(2029..3447)
                     /locus_tag="STRMOE7_RS35780"
                     /old_locus_tag="STRMOE7_35795"
     CDS             complement(2029..3447)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380533.1"
                     /locus_tag="STRMOE7_RS35780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35795"
                     /product="MFS transporter"
                     /protein_id="WP_084775737.1"
                     /transl_table=11
                     /translation="MPTTPPPGRAAPPGRSDPSQRIHQTRRSHPSRRSEASGAPRPSGS
                     PLLLLALGHACVDVYQGAVAALVPFFLAERAYTYAAASGIVLAASLLSSVAQPLFGALT
                     DKWPMPWLLPVSTLAGGVGVALSGVSGSYALTLAVVAVSGIGVAAYHPESARLARVASR
                     GSHTAMGWFSLGGNLGFATAPLAVSAVVATGGLHASPLLIVPALVGAALCVAALRTVNA
                     PTTTGRSASAVTRPAGKDDWPAFLRMSGAIVCRSIVFVGLSAFISLYARQRTGGGEVAG
                     TAALFVLYLGGAIGTVAGGRLATRYGRVRVVRWSYALTVPAVAGIVLVPGPLLYLFVAL
                     TSAGLYVPFSLHVTLGQDYLPRRVGTASGVTLGLTVSVGGIASPVIGAAADATSLQTAL
                     APLIALPALGWLLLRTLHEPAMREAAISEAAMPEAATPSAGLAEAAASEADAPDPARAV
                     ARAPHRDAARTTGP"
     misc_feature    complement(3482..3498)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.61"
                     /tool="antismash"
     gene            3502..4248
                     /locus_tag="STRMOE7_RS35785"
                     /old_locus_tag="STRMOE7_35800"
     CDS             3502..4248
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1260: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018844888.1"
                     /locus_tag="STRMOE7_RS35785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35800"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_084775738.1"
                     /transl_table=11
                     /translation="MPNIRHTPAAPTHTLALAAGERIDAHRHDNHQIVYAGSGVLAVTT
                     DAGTWFAPGTRAIWVPAGTVHAHRAYGHLDLHTLGLPVGDNPLRLDAPTVLAVSPLLRE
                     LILAYTRDPTDTGPARRRLRAVLLDQLRASPHEPVQLPTATDPRLAAVCALLHADPADP
                     GTLAEFGARTHTGERTLSRLFRSEFGMTFPQWRTQLRLYHALKMLADDLPVTAVAHRCG
                     WSSTSAFIDVFRRAYGYTPGTHNRRG"
     gene            complement(4293..4970)
                     /locus_tag="STRMOE7_RS35790"
                     /old_locus_tag="STRMOE7_35805"
     CDS             complement(4293..4970)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088744.1"
                     /locus_tag="STRMOE7_RS35790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35805"
                     /product="DUF5825 family protein"
                     /protein_id="WP_084775739.1"
                     /transl_table=11
                     /translation="MPPTTTAPARGALALSAWRDYDEDACALPGMSLGAVELTGPPEKY
                     ATRLWELGARRVRLAGPVDLTAVDDPDAAAHAVRQLCLVRDLTARAVLVEWHLRLPSQP
                     DDGWRDLSHLQPPRTLTGPADPAAVLTRWRDEHYLCKCLWRQGPGFVQIRDRRWGDLRR
                     FTADEPAYHEAISQLAYGAPPSAVPEAIAADFLEERLVARTGPLLWWLPYRVHRWIQEA
                     MAI"
     gene            complement(5001..6935)
                     /locus_tag="STRMOE7_RS35795"
                     /old_locus_tag="STRMOE7_35810"
     CDS             complement(5001..6935)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606751.1"
                     /locus_tag="STRMOE7_RS35795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35810"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_084775740.1"
                     /sec_met_domain="TIGR03975 (E-value: 1.3e-182, bitscore:
                     607.1, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLVNMPWSPIDLPSLALGILRRSVDERTSGHADVLHANLEFT
                     DWITRRTAFTGDDYQFYALSSYFMGCGDWVFSSALYDDPEWRVPEFSASMRGKLKDARL
                     RMSHELHRVVPEFVQEIAERIVAAGPDVVGFTSTFQQNTAALAAAKYVKRLAPHIRTVM
                     GGANCDAEQGAAAHRNFPFVDFVVRGEGEAAFPQLLTALDEGTELSAVPGLCHRGADGS
                     SIANPMSTSPLPPVAILPPDYSGYFERLASSVARNWVEPKLVVEGARGCWWGEKHHCTF
                     CGLNGSFMQFRSKSPDTFYKEIMDLARRHRVLDMYVVDNILDMGYLHTVLPRIIDSGYD
                     LRMHIEIKANMRRPQLRTLAEAGMIYVQPGIESLNSRVLDLMDKGVSGCQNVRMLRDGA
                     ETGLSVSWNYLHGFPGETAADYEPVIAQIPALEHLNPPVDLSARIAIERFSPYFNRPEL
                     GFTGLRPEEHYRFTYDLPESELLDLAYVFEAPERGIGEPTVTALNDALGAWKKRHVDSR
                     LTHADLGDRIVLVSRRHAFDWDVMELTRPTDIETFRLLDQPHTPAALTRKLAARLPEHA
                     LDAAAVHVLLQHWVTLGLVFTDNGQYVHLAPAAVNEDLLRLDFMRHTHAAAAPADQPDA
                     AARPVVAHA"
     gene            complement(7136..9106)
                     /locus_tag="STRMOE7_RS35800"
                     /old_locus_tag="STRMOE7_35815"
     CDS             complement(7136..9106)
                     /NRPS_PKS="Domain: Abhydrolase_1 (374-509). E-value:
                     2.3e-08. Score: 25.9. Matches aSDomain:
                     nrpspksdomains_STRMOE7_RS35800_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569707.1"
                     /locus_tag="STRMOE7_RS35800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35815"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_084775741.1"
                     /sec_met_domain="PF00561 (E-value: 7.1e-05, bitscore: 21.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 2e-34, bitscore:
                     117.6, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAGDFRVYQPALPEVCPRDPQRMVLAADADGRCEIFTWQAATGT
                     ARQVTDSRDGTLHCAIDADARVWWFAEDRNGRGRWYFEEFEGGGPRRPGLTGLRPGLPH
                     GLAVSDAGTVAIGLGDGRGTTVHIGTPGGPARPVATIDGPALLAGISPSGGLLALSGPA
                     GSERAVTLLTCAGAPLDRLSGRHGRLWALGFAPSTAGTELLLVQECGPESATPHEQQDV
                     RQHTVEGTPNGRYRLASWRPGSGLQPYDWCCFDTEITGRWYPEGRRVLIRQDRHGRSTL
                     VVADLDRRTLNPVPTPPGTVLDAVPHPGDDVHYLWTDTATPPRMRSTAGTRLPALATLP
                     GRAPGRHGELWTDGPDGPVHTLLSRPDPGPGGCPPLVFLVHGGPADHARDAYDPEVHSL
                     VASGFAVARVNYRGSTGYGPRWRAAFGEGVGLTQVADLVAVRADLLRRGLAREDAIGLW
                     GTSWGGYLALLALGTRPGLWQAGVAVKPVADCAAAHRSGTPALRALDERLFGGTPDTVP
                     ERYARSSPLHYAAEVRAPLLVVAATLDAKCPPGQVRGYLTALREAGVAHESLWLETGHD
                     GYTGAHHMTVLSRAVRFLDRHLRRSGTAHRTPCPSVGGVSGETGASGVPGLRGPIPDAV
                     HRQNRRSSHAEGHHPQRPARG"
     aSDomain        complement(7580..7984)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_STRMOE7_RS35800_Abhydrolase_1.1"
                     /evalue="2.30E-08"
                     /label="STRMOE7_RS35800_Abhydrolase_1.1"
                     /locus_tag="STRMOE7_RS35800"
                     /protein_end="509"
                     /protein_start="374"
                     /score="25.9"
                     /tool="antismash"
                     /translation="PLVFLVHGGPADHARDAYDPEVHSLVASGFAVARVNYRGSTGYGP
                     RWRAAFGEGVGLTQVADLVAVRADLLRRGLAREDAIGLWGTSWGGYLALLALGTRPGLW
                     QAGVAVKPVADCAAAHRSGTPALRALDERLF"
     gene            complement(9103..10380)
                     /locus_tag="STRMOE7_RS35805"
                     /old_locus_tag="STRMOE7_35820"
     CDS             complement(9103..10380)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088740.1"
                     /locus_tag="STRMOE7_RS35805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35820"
                     /product="MFS transporter"
                     /protein_id="WP_084775742.1"
                     /transl_table=11
                     /translation="MTPSAAAGAAASAVPLPHARRDVRLFWWAGTCDALGSQVSGLVLP
                     LLLLSLGWSPAEVGVVAGLSAAATLLLGPLAAVPADRGARKSVMVGAAVVSAAAMACVA
                     VTVGRGTVLPAVLLTAVLVERCATSCYEAASLGTVALVSPPGEHRRVLSRLQAGERGAL
                     VLGPALGGLLFHTGRWLPFLADALSYAVAAGCIRAMRADLAPAGEHRPPDGGPRRGPRA
                     LAAEAGAGVAVIRRDPFLRLAFVWLTAVNALLVALYYTTVFALQGGGRGATALGLVLAL
                     AGAAGLGGALLAPKLAGRHAAGRVLVPVSWLLVPPAAALATAREGWQFGLLFGLLCALT
                     PLATVALQARIIQVVPPVLQARTGTVLATAAGTGAALAPALAGLAADRAGAAVTFTGCT
                     LLLAVLALYATCAAPGLPRADAEGAA"
     misc_feature    complement(9916..9918)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    10478..10493
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.73"
                     /tool="antismash"
     misc_feature    10594..10608
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.33"
                     /tool="antismash"
     misc_feature    11915..11935
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.42"
                     /tool="antismash"
ORIGIN
        1 cttcgaggag gaaccgaaca cgaaagcacg gtcagggttc cccgcctcgt cgcaggcggc
       61 gagcaggtcg gcgatctcga cgggctcgtc ggtgaggtgg tgggcggcgt aggcgttgtc
      121 ccagaagatc cggaagtcgg gggccgcggt gttcatcgag gcgagccgcg ccaccgtcgc
      181 gtcgctgtag caggtgccgt ccgggttgct gtacttcggg acgcaccaga tgcccttgac
      241 cgtcgggtcc tcggcgacga gttgctccac gacgtccatg tccgggccct cggcggtcat
      301 cggcaccggg atcatgtcga tcccgaaccg ctcgcagagc gcgaagtgcc ggtcgtaacc
      361 gggcaccgga cagaggaacg cgatccgctc ctgctcgacc cagcgcgagg tggcgcccgg
      421 cagtacgccg agcagggcgt gcaccaggca gtcgtgcatc agctcaaggc tggagttccc
      481 tgcggcgagc agctgtccgg ccggcacctg gagcacctcg gcgaagatct cccgcagctc
      541 gggcagtccc tgcaggccgc cgtagttgcg tacgtccgtg ccgtcggcgg aggtgtgcac
      601 tccgcggggc aggctcagca gttcctcgga gaggtcgagc tgttccggtg ccggcttgcc
      661 ccgggtgagg ttgagggaga gcccgcgggc cgtgaggtcc tggtagtcct ggcgggcccg
      721 cgcaaggacc tcggtcaggg cgtcggggct cagctcggtg gtcatggtgc ttcctctcgc
      781 aggtgccgcg tcgaaggggg gcgcctgccg agaatacaaa ccgacgcggg tgggcccggg
      841 agctgaacgc tatgtggacg ccgtgacctc ggagagtgat atccgcgtcc gcatacggta
      901 gatggggcgt atgcgacctt tacggtcgat gctgcctata aggtccgtgc ggagccgggc
      961 gacttccagg ccggatgtcc gagagttcgg acgccggtac gggcggtgac ggagactgct
     1021 tgtatgcact tgccgacctc catcgacgtt cccgaggcgt tcgccgcgtc ctacggtggg
     1081 cagggtgccg aggcgcgggc ctggctcgcc gggctgcccc gcctcggcgg tgacctcctg
     1141 gagcgctggg acctgcggcc cgacggtccc gccgggcacg gcatggcctc gctcgtcctg
     1201 cccgtgctcc gagccgacgg cacccccgcc gccctcaagc tccagcagct ccgggaggag
     1261 accgccggcg cggccaccgg tctgcggacg tggcggggca acggcatcgt ccgcctcctc
     1321 gaccacgacg aggagtccag cagccagctg ctcgaaaggc tcgacgccac ccgcaccctc
     1381 tcctcggtcg ccgacgacgc ccgggccctg cggatcctcg ccgagctgtt ggcccggctg
     1441 tcgtcggtgc ccgcccccga gggcctgcgc acgctgtccg gcctcgccgc cgccatgctc
     1501 gaccaggtcc cgcgggcagt gccggcgctg cgcgatcccg ccgaacagcg gctggtacgc
     1561 acctgcgcgt ccgccgtcgc cgagctggtc ggtgaacccg gcgaccggct gctgcactgg
     1621 gacctgcact acgacaacgt cctcgcgggc gagcgggaac cctggctggc catcgacccc
     1681 gagccgctgg ccggggatcc cgggttcgag ctgctgccgg cactgcacaa ccggtgggcg
     1741 gaggtggtgg cgacgggcga cgtcgaacgt gcggtgctgc gccgcttcga cctgctgacc
     1801 gaggccctcg gcctggaccg gcggcgggcc accggctgga cgctcggccg tgtgctgcag
     1861 aacgcgctgt gggacatcga agacggcaag acgcacctcg aacctgcgca gatcgccatc
     1921 gccggggccc tgttgggccg tgcaacgggc tgaggtgctc ctaccggagc gccccggggg
     1981 gcccgctgcc ctcgggcctt ccggccggtg gcaccgaggg ctgggacgtc aggggcccgt
     2041 ggtgcgcgcg gcgtcgcggt gcggcgcgcg ggcgacggcc ctcgcggggt ccggagcatc
     2101 ggcttcggac gcggcggctt cggccaggcc agctgacggc gtggccgcct cgggcatcgc
     2161 ggcctcggat atcgccgcct cgcgcatggc gggctcgtgc agggtgcgca acagcagcca
     2221 gcccagggcg ggcagcgcga tgagcggggc gagagcggtc tgcagggacg tcgcgtcggc
     2281 cgcggcgccg atgacggggc tggcgatgcc gccgacgctg accgtcaggc ccagggtgac
     2341 gccgctggcg gtgccgacgc gccgcggcag gtaatcctgg ccgagggtga cgtgcaggga
     2401 gaagggcacg tacagaccgg cggaggtcag cgcgacgaac aggtacagca gcggcccggg
     2461 gaccagcacg atgccggcga cggcgggcac ggtcagcgcg tacgaccagc gcaccacccg
     2521 gacgcggccg taacgcgtcg cgagccggcc gcccgcgacg gtgccgatcg cgccgccgag
     2581 gtagagcacg aacagggccg cggtgccggc gacttcgccc cctccggtgc gctgccgcgc
     2641 gtagagggag atgaaggcgc tcaggccgac gaagacgatc gagcggcaca cgatggcccc
     2701 ggacatcctg aggaaggccg gccagtcgtc cttgcccgcc gggcgggtga cggcggaggc
     2761 ggaacgtccg gtcgtagtgg gggcgttgac cgtacgcagg gcggccacgc acagcgcggc
     2821 gcccaccagg gccggaacga tcagcagcgg tgaggcgtgc aggccaccgg tggcgacgac
     2881 ggcggagacg gcgagcgggg cggtggcgaa gccgagattg cccccgaggg agaaccagcc
     2941 catcgcggtg tgactgcccc ggctcgcgac gcgggccagc cgggcggact cgggatggta
     3001 cgccgccacc ccgatcccgg acaccgcgac caccgccagc gtcagcgcgt acgagccact
     3061 gacgccgctg agcgccacac cgacgcctcc ggccagcgtg ctcaccggca gcaaccacgg
     3121 catcggccac ttgtcggtga gcgccccgaa cagcggctgg gccaccgagg agagcagcga
     3181 ggcggcgagg acgatgccgg acgccgcggc gtaggtgtag gcccgctcgg cgaggaagaa
     3241 cggtacgagg gcggccaccg cgccctgata gacgtccaca caggcgtgcc ccagggccag
     3301 cagcaggaga gggcttccgg acggacgtgg tgcaccggac gcctcggacc ggcgggacgg
     3361 gtgggaccgg cgggtctggt ggatccgctg ggacgggtcg gatcggccgg gcggagcggc
     3421 ccggccgggc gggggagtcg ttggcatgtc tcgatcgtca cggccgccgc ggctgtcgtg
     3481 cttccgataa aatgccaagg tatgccgaac atccgccaca cccccgcggc gccgacccac
     3541 accctcgcgc tggcggccgg agaacgcatc gacgcgcacc gccacgacaa ccaccagatc
     3601 gtctacgcgg gttccggcgt gctggccgtc accaccgatg ccgggacgtg gttcgcgccg
     3661 ggtacccgag ccatctgggt gcccgccgga accgtccacg cccaccgcgc ctacggccac
     3721 ctcgacctgc acaccctcgg cctgcccgtc ggcgacaacc cgctgcgcct cgacgcgccc
     3781 accgtcctcg ccgtcagccc cctgctgcgc gagctgatcc tcgcctacac ccgcgacccc
     3841 acggatacgg gtcccgcgcg ccgccggctg cgcgcggtgc tcctggacca gctccgcgcg
     3901 tctccgcacg agcccgtgca gctgcccacg gccaccgacc cgcgcctcgc cgccgtctgc
     3961 gcgctcctgc acgccgaccc cgccgacccc ggcacgctcg ccgagttcgg cgcccgcacg
     4021 cacaccgggg agcgcaccct cagccgcctc ttccgctccg aattcggcat gacgtttccg
     4081 caatggcgca cccaactgcg cctctaccac gccctgaaga tgctggccga cgacctcccc
     4141 gtcaccgccg tcgcccaccg ctgcggctgg tcctccacca gcgccttcat cgacgtcttc
     4201 cgccgcgcgt acggctacac gcccggcacc cacaaccgcc ggggctagct gccgggccgc
     4261 tcggcttcgg cgttgctttt cctgacggcc gttcagatgg ccatcgcctc ctggatccag
     4321 cgatggaccc ggtacggcag ccaccacagc agcgggccgg tgcgggcgac cagtcgctcc
     4381 tccaggaagt cggcggcgat cgcctccggt acggccgatg gcggtgcgcc gtaagccagt
     4441 tggctgatcg cctcgtggta ggcgggctcg tccgcggtga agcggcgcag atcgccccag
     4501 cggcggtcgc ggatctgcac gaagccgggg ccctggcgcc acaggcactt gcagaggtag
     4561 tgctcgtcgc gccaccgcgt caggacggcc gccgggtcgg cggggccggt cagggtgcgg
     4621 ggcggttgca gatggctgag gtcgcgccag ccgtcgtcgg gctgcgacgg cagccgcagg
     4681 tgccactcca ccagaacggc gcgggcggtc agatcgcgta ccaggcacag ctgccggacg
     4741 gcgtgcgcgg cggcgtccgg gtcgtccacc gcggtgaggt cgacggggcc ggcgagccgg
     4801 acgcggcgcg ccccgagctc ccacagccgg gtggcgtact tctccggcgg gccggtcagt
     4861 tcgactgcgc ccaggctcat gccgggcagt gcgcaggcgt cctcgtcgta gtcgcgccat
     4921 gcgctgagtg ccagcgcgcc tcgggcgggg gcggtggtgg tagggggcat gggggtctcc
     4981 tctccgggcc gggtgggtgc tcaggcgtgg gcgacgacgg ggcgggccgc cgcgtccggc
     5041 tggtccgccg gggccgccgc ggcgtgggtg tggcgcatga agtcgaggcg cagcagatcc
     5101 tcgttgacgg cggcgggggc cagatggacg tactggccgt tgtcggtgaa gaccaggccg
     5161 agcgtcaccc agtgctggag gaggacgtgg acggcggcgg cgtcgagggc gtgctccggc
     5221 aggcgcgcgg cgagcttgcg ggtgagcgcg gcgggggtgt gcggctggtc gaggagccgg
     5281 aacgtctcga tgtcggtggg gcgggtgagt tccatgacgt cccagtcgaa ggcatgccgc
     5341 cggctgacca ggacgatgcg gtcaccgagg tcggcgtgcg tgagcctgct gtccacgtgg
     5401 cgtttcttcc acgcgccgag ggcgtcgttg agggcggtga cggtgggctc gccgatgccg
     5461 cgctcgggag cctcgaagac gtaggccaga tcgagcagtt cggactccgg caggtcgtag
     5521 gtgaagcggt agtgctcctc gggccgcagg ccggtgaacc ccagctcggg ccggttgaag
     5581 tacggactga aacgctcgat cgcgatgcgg gccgacaggt cgaccggcgg gttgaggtgc
     5641 tccagagccg gtatctgcgc gatgaccggc tcgtagtcgg cggcggtctc cccggggaag
     5701 ccgtggaggt agttccagga gacggacagc ccggtctcgg ctccgtccct gagcatgcgg
     5761 acgttctggc agccgctgac gcccttgtcc atcaggtcga gcacccggct gttcaggctc
     5821 tcgatgcccg gctgcacgta gatcatcccg gcctcggcga gggtgcgcag ctggggccgg
     5881 cgcatgttcg ccttgatctc gatgtgcatc cgcaggtcgt agccgctgtc gatgatgcgg
     5941 ggcagcacgg tgtggagata gcccatgtcg aggatgttgt cgacgacgta catgtccagc
     6001 acccggtggc ggcgtgccag gtccatgatc tccttgtaga aggtgtcggg gctcttgctg
     6061 cggaactgca tgaaggagcc gttgaggccg cagaacgtgc agtggtgttt ctcgccccac
     6121 cagcagccgc gcgccccctc gacgacgagc ttgggctcca cccagttgcg ggcgacggac
     6181 gacgccagcc gctcgaagta gccgctgtag tcgggcggca ggatggcgac gggcggcagc
     6241 gggctggtgc tcatcgggtt ggcgatgctg gagccgtcgg cgccgcggtg gcacaggccg
     6301 ggtacggcgg acagctccgt gccctcgtcg agcgcggtca gcagctgcgg gaaggcggcc
     6361 tcgccctcgc cgcggaccac gaagtccacg aacgggaagt tgcggtgcgc ggcggcgccc
     6421 tgctcggcgt cgcagttcgc cccgcccatg accgtccgga tgtgcggggc gaggcgtttg
     6481 acgtacttgg cggcggcgag cgcggcggtg ttctgctgga aggtggaggt gaagccgacg
     6541 acgtcgggac cggccgcgac gatccgctcg gcgatctcct gcacgaactc aggtaccacc
     6601 cggtgcagtt cgtgcgacat ccgcagccgc gcgtccttca gcttgccgcg catcgacgcg
     6661 ctgaactccg gtacgcgcca ctcgggatcg tcgtagaggg cggaggagaa gacccagtcg
     6721 ccgcacccca tgaagtacga ggacagcgcg tagaactggt agtcgtcgcc ggtgaacgcg
     6781 gtgcgccggg tgatccagtc ggtgaactcc agattggcgt gcagtacgtc ggcgtgaccg
     6841 gaggtgcgct cgtcgacgct ccgccggagg atgccgagcg cgagggacgg cagatcgatc
     6901 ggggaccagg gcatgttgac cagcagaacg cgcatggcgg ctcctcgtac ggcggcggat
     6961 gggcaagggg cggccgccac gcgtcaccgg ccggcgtcgg tcgggtgacg acgggcggcc
     7021 ggccggcccg gtggcgtacc gggccggccg gggaggtcac tcctcctcgg tgtcctccgc
     7081 ggtgcggaac ggcacggtca ccgtgatgcc gatgcccggc ttccggttct cctcgtcacc
     7141 cgcgagcggg tcgttgtgga tgatgtcctt ctgcatggct gcttctcctg ttctgccggt
     7201 gtacggcatc gggaatcggg cctcgcaacc cggggacacc ggatgcgccg gtttccccgg
     7261 agaccccgcc cacggagggg cagggagttc ggtgggccgt accggagcgg cgcaggtgcc
     7321 ggtccaggaa ccgcacggcg cggctcagta cggtcatgtg gtgggcgccg gtgtagccgt
     7381 cgtggccggt ctccagccac agggactcgt gcgcgacgcc ggcctcccgc agtgcggtga
     7441 gatagccgcg cacctgaccc ggcgggcatt tggcgtcgag cgtggcggcg acgaccagca
     7501 gcggagcgcg tacctcggcg gcgtagtgga gcggcgagct ccgcgcgtag cgttcgggca
     7561 ccgtgtcggg ggtgccgccg aacagccgct cgtccagggc ccgcagcgcg ggggtaccgc
     7621 tgcggtgcgc cgccgcgcag tccgcgaccg gtttgacggc gacgcccgcc tgccacagac
     7681 cgggccgggt gccgagcgcc agcagggcca ggtagccgcc ccaggaggtg ccccacagcc
     7741 cgatggcgtc ctcgcgggcc aggccgcggc gcagcagatc ggcgcggacc gcgacgaggt
     7801 cggcgacctg ggtcagcccg acgccctcgc cgaacgcggc ccgccagcgg gggccgtacc
     7861 cggtcgaacc gcggtagttg acccgcgcga ccgcgaatcc cgacgcgacc agcgagtgca
     7921 cctccgggtc gtaggcgtcg cgggcgtggt cggcgggccc gccgtggacg aggaaaacca
     7981 gcggggggca cccgccgggc ccggggtccg gcctgctgag cagggtatgg acgggcccgt
     8041 ccgggccgtc ggtccacaac tcgccgtgcc ggccgggcgc gcgaccgggc agcgtggcga
     8101 gggccggcag ccgggtgccg gcggtggagc gcatccgggg cggggtggcg gtgtcggtcc
     8161 agaggtagtg cacgtcgtcg cccggatgcg gaacggcgtc gagcacggtg cccggcggcg
     8221 tgggcaccgg gttcagggtg cgccggtcga ggtcggcgac gacgagcgtg gagcgcccgt
     8281 gccggtcctg acggatgagg acccgacgcc cctcgggata ccagcgaccg gtgatctcgg
     8341 tgtcgaagca gcaccagtcg tacggctgaa ggccgcttcc gggacgccaa ctggccagtc
     8401 gatagcgacc gttgggtgtg ccctcgaccg tgtgctgccg gacgtcctgc tgctcgtgag
     8461 gcgtcgcgga ctccggtccg cattcctgta cgagcagcag ttcggtgccc gccgtcgacg
     8521 gggcgaagcc cagcgcccac agccggccgt gccgcccgga gagccggtcg aggggtgccc
     8581 ccgcgcaggt cagcagggtg actgcgcgct cggaaccggc cggcccggac agcgccagca
     8641 gcccgcccga gggcgagatg cccgccagca gcgcagggcc gtcgatggtc gccaccggcc
     8701 gtgccgggcc gcccggcgtc ccgatgtgga cggtcgtgcc gcggccgtcg ccgaggccga
     8761 tggccaccgt cccggcgtcg ctgacggcga gcccgtgcgg gaggcccggt cgcagaccgg
     8821 tcaggccggg gcgccggggc ccgccgccct cgaactcctc gaagtaccac cgcccgcggc
     8881 cgttgcggtc ctccgcgaac caccacaccc gggcgtcggc gtcgatggcg cagtgcagcg
     8941 ttccgtcgcg gctgtcggtg acctggcggg cggtgccggt ggcggcctgc caggtgaaga
     9001 tctcgcagcg gccgtccgcg tccgcggcca ggaccatgcg ctgcgggtcg cgcgggcaga
     9061 cctcgggcag cgccggctgg tagacccgga agtcgccggc ggtcatgcgg ccccctcggc
     9121 gtcggcgcgg gggaggcccg gcgccgcgca ggtcgcgtac agggcgagca ccgccagcag
     9181 cagggtgcag ccggtgaagg tgacggcggc cccggcccgg tcggcggcca gcccggcgag
     9241 ggccggcgcc agagccgcac cggtccccgc cgccgtggcc agcacggtgc cggtgcgggc
     9301 ctgcagcacc ggcgggacga cctggatgat ccgggcctgc agcgccacgg tggccaacgg
     9361 cgtcagcgcg cacagaaggc cgaagagcag cccgaactgc cagccctcgc gggccgtcgc
     9421 cagggcggcg gcgggcggca ccagcagcca cgacaccggc accagtaccc gccccgcggc
     9481 gtgccggccg gccagcttgg gcgcgagcag cgcgccaccg aggcccgccg cgccggcaag
     9541 cgcgagcacc agccccagcg cggtcgcgcc ccggccaccg ccctgcaacg cgaagaccgt
     9601 tgtgtaatac agcgcgacga gcagggcgtt gacggccgtg agccagacga acgccagccg
     9661 caggaaggga tcgcggcgga tcacggcgac gcccgccccg gcctcggccg ccagcgctcg
     9721 cggtccgcgg cgcgggccgc cgtccggtgg cctgtgctcc ccggccgggg cgagatccgc
     9781 gcgcatggca cggatgcagc ccgccgcgac ggcgtaggag agcgcgtccg cgaggaacgg
     9841 cagccaccgg ccggtgtgga agagcagccc gcccagcgcc ggcccgagaa cgagggcgcc
     9901 gcgctctccc gcctgtaatc gggacagcac gcgccggtgt tcgccgggcg gactcaccag
     9961 cgcgacggtc ccgagcgagg ccgcctcgta gcacgaggtc gcacaccgct cgacgaggac
    10021 ggcggtcagc agcacggccg gcagcaccgt gccccgcccc acggtcaccg cgacgcaggc
    10081 catcgcggcc gcggacacca ccgcggcacc caccatcacg ctcttgcgtg caccgcggtc
    10141 ggcgggcacc gcggcgagcg gccccagcag cagcgtggcg gccgcggaca gcccggcgac
    10201 cacacccacc tcggccgggg accagcccag actcagcagc agcaacggca gtacgagccc
    10261 ggacacctga ctgcccagcg cgtcgcaggt cccggcccac cagaacagcc ggacgtcacg
    10321 gcgggcgtgc ggcagcggta cggcggacgc tgcggccccc gcggcggcgg acggcgtcac
    10381 cggatacgac caagggccga gggcataccg gccaccgggc cggagggtaa gccggacggt
    10441 aagtcgtccg ccggggacgc cgctgatcgc cgttccatgc gagccacctc acgtcaatgt
    10501 gggcagatcg tgaagatcgg ctcactaaat aggtcaactt gcggaggaaa tgtgcccgaa
    10561 acgtgccgga gcttagccat ggagtgactc aggccacaag ccgtggggag gtgaatgtcc
    10621 gcagaagtca cgaccgttga ggccgccggt gctcacgacg gcgccacgac gggccggcga
    10681 ggggccgaaa acgggcccct acgccctcga caggctctgc gcgaagtcga agacccggtc
    10741 cgggtcgtag tcgtgcgcga ccgaacgcag gcgttccaga ttcgggccgt agtaggaccg
    10801 gggccagtcg ggcagggccg gatcgatgta gttcacgtag ccgccgccca tgcccagctt
    10861 cgccagcccg gccacgacct cggcgacgct gcgcgtgact tcagcctcgg acgcagccga
    10921 cgcggtcgcg tagatctgtg cggtggcaaa ggcggtgcgg tgcggaaagg ccgtctgctg
    10981 cacgccgacg tcggccaccg ccccgccgag cgcgtcgacg atgagcgaca tgcccgtgcg
    11041 gccggtgagc acggcgacca ccgcgcccgg gtcgggggcg ggtttgctca gcacccgcga
    11101 cgacgcgacg aacgactcac ggtccggttt gccggagaag aagcgcatgg ccctgaagta
    11161 gtcccgctcg tcgaccgtgc gccggtcggg catgactccg gagcgcgaga tcagatcgtc
    11221 cagcaggggg tggcaggtac tgctcggccc gacgaagcag ccggccaccc ggcagctcgg
    11281 cgggtcgccg cccgacatgt tgaagttggc ccacagctcg ttgggcgcgg cgctgatcca
    11341 gggctgccac gcgcccagca ccttggccgt cgacccggcc gggaagacga tcacgaacac
    11401 ggtcagcgtc ggtgccggat cggttgcgaa ggtgaaccgc gtgacgatgc cgaaattgcc
    11461 gcccccgccg ccgcgcagcg cccagaacag atcgggggta cgcgcggcgg acaccgtgag
    11521 cagccgtccg tcggcggtga cgacgtcggc ggacaccagg tgatcacagg tcagcccgaa
    11581 cttccgttgc agtacgccga ttccgccgcc cagcgtcacg cccgaaatgc cgacggtgaa
    11641 gcaggagccg ccgggcagac accggttcgc ctggccgagc ccgctgtaga cgtcccgcag
    11701 gcaggcgccc gccccgacgc tcgcggtgcc gtccgcctgg accgccaccg ccgacatgcg
    11761 gcgcaggtcg agaaccaggc cgcggtgcgg cgccgagtag ccgacgtagg agtgcccgcc
    11821 gctgcgggcg gcgagcggca cccggttcgc ggccgccagc actccttcgc gcacgtcctc
    11881 ggcggactcg cactgcgcca cggccgcggg gatgtgatcg tcgttgagcg gattg
//
