LOCUS       NZ_CP019779            10966 bp    DNA     linear   CON 14-MAR-2024
DEFINITION  Streptomyces sp. MOE7 chromosome, complete genome.
ACCESSION   NZ_CP019779
VERSION     NZ_CP019779.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MOE7
  ORGANISM  Streptomyces sp. MOE7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8399509)
  AUTHORS   Elnahas,M.O., De Leon,K.B., Amin,M.A., Hussein,M.M.D., Ali,A.E. and
            Wall,J.D.
  TITLE     Complete Genome Sequencing of Streptomyces sp. Strain MOE7, Which
            Produces an Extracellular Polysaccharide with Antioxidant and
            Antitumor Activities
  JOURNAL   Genome Announc 5 (22), e00442-17 (2017)
   PUBMED   28572320
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399509)
  AUTHORS   Elnahas,M.O., De Leon,K.B., Amin,M.A., Hussein,M.M.D., Ali,A.E. and
            Wall,J.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2017) Biochemistry, University of Missouri
            Columbia, 117 Schweitzer Hall, Columbia, MO 65211, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 8.1
            Genome Coverage                   :: 180.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002090335.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 02:19:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,435
            CDSs (total)                      :: 7,344
            Genes (coding)                    :: 7,005
            CDSs (with protein)               :: 7,005
            Genes (RNA)                       :: 91
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 339
            CDSs (without protein)            :: 339
            Pseudo Genes (ambiguous residues) :: 0 of 339
            Pseudo Genes (frameshifted)       :: 230 of 339
            Pseudo Genes (incomplete)         :: 155 of 339
            Pseudo Genes (internal stop)      :: 7 of 339
            Pseudo Genes (multiple problems)  :: 47 of 339
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:50:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8006226
            Orig. end                         :: 8017192
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019779.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Judy D. Wall 117 Schweitzer
            Hall Columbia, MO USA 65202.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10966
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="28"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10966
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10966
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5966](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5966)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(367..1560)
                     /locus_tag="STRMOE7_RS35090"
                     /old_locus_tag="STRMOE7_35105"
     CDS             complement(367..1560)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019074505.1"
                     /locus_tag="STRMOE7_RS35090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35105"
                     /product="cytochrome P450"
                     /protein_id="WP_084775629.1"
                     /sec_met_domain="p450 (E-value: 2.4e-22, bitscore: 78.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAPNPLESAGFSRDPYPLLAALRSQGPVQRMPTGNGRTTWVVTGW
                     AEARAALADARLSKDTARYFADRPSNRRLAPAVSRSMLATDPPHHGRLRKLAMTAFTPA
                     AVARLAPRIRALAEELADALAARGSGDLIEEFAVPLPIAVISELLGVPEAERAAVRTWS
                     NDLFAAAAPETVDRASHALSDYMTGLIASRRAAPGDDVLSGLIAARDEDDRLSEAELVS
                     LAVLLVVAGHETTTHLIGNGMLALLKDDALRARLRAEPALLPAAVEEFLRHDAPLTLAT
                     FRYATEPVELGGARIATGEVVLVSPGAANRDPARFAAPDEVRLDPPDQAGRHLSFGHGP
                     HHCLGAPLARLEARIAFEVLLTRFADIRPASADWDELEWRRTRLMRGLVRLPVHIDR"
     gene            1654..1881
                     /locus_tag="STRMOE7_RS35095"
                     /old_locus_tag="STRMOE7_35110"
     CDS             1654..1881
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608014.1"
                     /locus_tag="STRMOE7_RS35095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35110"
                     /product="hypothetical protein"
                     /protein_id="WP_033267439.1"
                     /transl_table=11
                     /translation="MTEHPPVIVHPPTPSGGRRVTVRGQIVGLAHGRGDVAEFLRRAGV
                     AGPAEDIRLDDPRLVEWRGGSLDDWPMPPA"
     gene            2121..2312
                     /locus_tag="STRMOE7_RS35100"
                     /old_locus_tag="STRMOE7_35115"
     CDS             2121..2312
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608013.1"
                     /locus_tag="STRMOE7_RS35100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35115"
                     /product="hypothetical protein"
                     /protein_id="WP_077194337.1"
                     /transl_table=11
                     /translation="MSDGDSGNSGDSGASGDSGDYNDASAGHSRGSGSPSWDVLCRAMR
                     WETLALLVFLVVLAALAS"
     gene            2702..3229
                     /locus_tag="STRMOE7_RS35105"
                     /old_locus_tag="STRMOE7_35120"
     CDS             2702..3229
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092054.1"
                     /locus_tag="STRMOE7_RS35105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35120"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_084775630.1"
                     /transl_table=11
                     /translation="MTLYTTLDSPLGELLLVGEESATAPGGTALVSLSVPDQKGGVTVQ
                     DGWQHAPGAFTEITSQLRSYFDGTLTRFSIEYANTASTRGTGFQQRVWQALESIPYGTT
                     TTYGRIAADIGAARGAVRAVGTAIGANPLLVVRPCHRVIAANGALSGYAGGPARKRQLL
                     GLEGVVPNGSTP"
     gene            3226..3996
                     /locus_tag="STRMOE7_RS35110"
                     /old_locus_tag="STRMOE7_35125"
     CDS             3226..3996
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016642486.1"
                     /locus_tag="STRMOE7_RS35110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35125"
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="WP_084775631.1"
                     /transl_table=11
                     /translation="MTATTTKNRAGTRTAPGPARRSAALAARVAATDWAALGEELDTVG
                     CALTPRLLTPADCRALIALYDEAERFRSTIDMARHRFGSGQYRYFAEPLPEPVRALREA
                     FYPHLLTVARAWADKLGRPAPWPDTLEEWLQMCHAAGQTKSAQILLRYGAGDWNALHRD
                     LFGERVFPLQVVIGLDEQGTDYEGGEFLLVEQRPRAQSRGTTTVLPQGHGLVFTTRDRP
                     VRSARGWSAGAVRHGVSTVRAGRRHSLGLVFHDA"
     gene            complement(4082..4846)
                     /locus_tag="STRMOE7_RS35115"
                     /old_locus_tag="STRMOE7_35130"
     CDS             complement(4082..4846)
                     /NRPS_PKS="Domain: PKS_KR (7-175). E-value: 6.9e-10. Score:
                     31.1. Matches aSDomain:
                     nrpspksdomains_STRMOE7_RS35115_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608009.1"
                     /locus_tag="STRMOE7_RS35115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35130"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_084775632.1"
                     /transl_table=11
                     /translation="MGDLNGKTALVTGSSRGIGRGIAQRLAQDGALVAVHYGHNDTAAK
                     ETAETIRGAGGRAFIIGAELGVPGDAETLFAAFDDGLAACGAEPGLDILVNNAAISLPG
                     HIDEVTPEEFDRTLAVNTKAPFFLIQHGLRRMRDGGRIINISSAVTTTAFPSTIAYGVS
                     KGAVDTLTLTLAKDLGARGITVNTIAPGFVATDMNAAMRATPEAEAALASISVFNRLGR
                     PADIADVAAFLASDDSRWITGQRFDVTGGSML"
     aSDomain        complement(4322..4825)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_STRMOE7_RS35115_PKS_KR.1"
                     /evalue="6.90E-10"
                     /label="STRMOE7_RS35115_PKS_KR.1"
                     /locus_tag="STRMOE7_RS35115"
                     /protein_end="175"
                     /protein_start="7"
                     /score="31.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGSSRGIGRGIAQRLAQDGALVAVHYGHNDTAAKETAETIR
                     GAGGRAFIIGAELGVPGDAETLFAAFDDGLAACGAEPGLDILVNNAAISLPGHIDEVTP
                     EEFDRTLAVNTKAPFFLIQHGLRRMRDGGRIINISSAVTTTAFPSTIAYGVSKGAVDTL
                     TLTLA"
     CDS_motif       complement(4754..4819)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_STRMOE7_RS35115_0001"
                     /evalue="1.10E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="STRMOE7_RS35115"
                     /protein_end="31"
                     /protein_start="9"
                     /score="11.7"
                     /tool="antismash"
                     /translation="LVTGSSRGIGRGIAQRLAQDGA"
     gene            complement(5001..5966)
                     /locus_tag="STRMOE7_RS35120"
                     /old_locus_tag="STRMOE7_35135"
     CDS             complement(5001..5966)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608008.1"
                     /locus_tag="STRMOE7_RS35120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35135"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_084775633.1"
                     /sec_met_domain="AfsA (E-value: 3.3e-18, bitscore: 64.3,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSETMHVSGTVQPKAPHRTRLIEKPCGTPRVPREFVHRPIVDDIL
                     VTSWRRQDDSHFSVTAQWPEEHGYFASQDGRHHLILTGETIRQAGLLLSHTELGVPVGH
                     HFILGDLDYTTYPEHLDAGSGPIGLSIDVSCSKMRMRAGTLAGARFDMTLRAADRIVAT
                     GHSHVTVASPAVYRRIRGEQLTARRTLGPLPTCVPPRLTGSTSEHDVLLSPTDRPDSWL
                     LRIAPGHAAVVNPANDHVPGMVLLDAAQQAAHALTAPGTFVPYAFGTTFHRYAEHGTPC
                     AIEARRVPSALPGTTAVQVTGSQDGHPVFVSTLTARDSRG"
     misc_feature    complement(6028..6041)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.3"
                     /tool="antismash"
     gene            6140..6820
                     /locus_tag="STRMOE7_RS35125"
                     /old_locus_tag="STRMOE7_35140"
     CDS             6140..6820
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092050.1"
                     /locus_tag="STRMOE7_RS35125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35140"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_084775634.1"
                     /transl_table=11
                     /translation="MAQQDRAIRTRRVILEAAASVFDEQGYDRATIAEVLERAGVTKGA
                     LYFHFASKEQLALAVLEEQVVDIAVEPQKIKLQEFVDAGQVLAYRLRRDPIQRGAARLA
                     VEQGSNHLDRKQSMISWTRFVEGLLNEARARGEILESIVVRDTAELFVGAFAGLQMMSH
                     AMSNQADLSHRLTVFFEHTLPSIAVPAVLAKLNLDPARGEKLEAALQRKMSAAAEAGES
                     VAVG"
     misc_feature    complement(7105..7120)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     misc_feature    7106..7119
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.18"
                     /tool="antismash"
     gene            7238..9657
                     /locus_tag="STRMOE7_RS35130"
                     /old_locus_tag="STRMOE7_35145"
                     /pseudo=""
     CDS             7238..9657
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537753.1"
                     /locus_tag="STRMOE7_RS35130"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_35145"
                     /product="MFS transporter"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTQTTTDQLPGRTASTAAAPPVPEPGAGGHRRARGGIVPVLAFSG
                     IVVAVMQTLLVPVIKDLPVLLNTAPSNATWVMTATLLAGPSRRPSWGGSATSTASGGCC
                     "
     gene            10415..10750
                     /locus_tag="STRMOE7_RS35135"
                     /old_locus_tag="STRMOE7_35150"
     CDS             10415..10750
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="STRMOE7_RS35135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="STRMOE7_35150"
                     /product="hypothetical protein"
                     /protein_id="WP_084775635.1"
                     /transl_table=11
                     /translation="MISDRNTVRYNVSINDRNTTAPYGVISVVCAPATRTLVHHNTVTT
                     NAPDTAVVSSNGPGGVTFRDDVFAGAAAGSPLADARNTYDHNLNLYDHNLYARISPVPA
                     TDAGAVR"
ORIGIN
        1 tcgtcccaca tgtgccggac gatcacctcg gacgcccgca cccgccgctc caccggccac
       61 ccggcgcccg caccgaacac ccacgccaca tggccgcccg cctcctcgaa cggcacggtg
      121 tggctgtcga actccttgac cagcccgatg tcgtgcagca tcgccgagac gtacagcagc
      181 tccgggtcga aggcgatgtc gtgcagcgac gcgtaggccg ccgcccacag gtaggcccgt
      241 acggagtggt tgagcagtgc gggggagcag taggcgctcg ccacctccag cgcggcgcgg
      301 cagacccggg tctccggcat cacaagatca tccatcgtca tggccttgat tcttgcgcac
      361 gacggatcac cggtcgatgt gcaccggcag gcgcaccagc ccccgcatca ggcgcgtccg
      421 ccgccactcc agctcgtccc aatccgcgga cgccggccgg atgtcggcga aacgtgtcag
      481 cagcacctcg aacgcgatcc gggcctccag acgggccagg ggtgcgccga ggcagtgatg
      541 cgggccgtgt ccgaaggaga ggtgccggcc ggcctggtcg ggggggtcca gccgtacctc
      601 gtcgggcgcc gcgaaacgcg ccggatcgcg gttggccgcg ccgggggaga ccaggaccac
      661 ctcgcccgtc gcgatccgcg ccccgcccag ctcgaccggc tcggtggcgt agcggaaggt
      721 cgcgagggtg aggggcgcgt cgtggcgcag gaactcctcg accgcggcgg gcagcagcgc
      781 gggctcggcc cgcagccggg cgcgcagcgc gtcgtccttg agcagggcca gcatgccgtt
      841 gccgatgagg tgggtggtcg tctcgtggcc cgcgacgacc aacagcacgg ccagcgacac
      901 cagttcggcc tcggacagtc ggtcgtcctc gtcgcgggcg gcgatcagac cgctcagcac
      961 atcgtcgccg ggggccgcgc gccgggacgc gatcagcccc gtcatgtagt cgctgagcgc
     1021 gtgcgaggcg cggtcgacgg tctccggggc cgccgcggcg aacaggtcgt tggaccacgt
     1081 gcgtaccgcc gcccgctcgg cctccggcac cccgagcagc tcgctgatca ccgcgatcgg
     1141 cagcggcacc gcgaactcct cgatgaggtc cccggacccg cgcgcggcca gtgcgtcggc
     1201 cagttcctcg gcgagcgcgc ggatccgggg cgcgagccgc gcaacggccg ccggggtgaa
     1261 cgccgtcatc gccagcttgc gcagtcttcc gtggtgtggc gggtcggtgg cgagcatgga
     1321 ccggctgacc gcgggggcca gccggcggtt gctcggccgg tcggcgaagt agcgtgcggt
     1381 gtccttcgac agccgcgcgt cggccagcgc ggcccgcgcc tcggcccacc ccgtgacgac
     1441 ccatgtcgta cggccgttac cggtcggcat ccgctggacg gggccctgcg accgcaaggc
     1501 ggcgagcagc ggatacgggt cccgggagaa cccggccgat tccagggggt tgggtgccat
     1561 ccggccagcg tagcgagtgg ttgcgggcgc aaggatcgcg ctggtggcgg ctgtcgaggt
     1621 gcgcgagcgc ggagcgcgga gcaggctgga ggcatgaccg agcatccgcc tgtcatcgtg
     1681 cacccgccga ccccctccgg tggccgacgg gtgaccgtgc gtgggcagat cgtcgggctg
     1741 gcccacggcc gcggcgatgt ggccgaattc ctgcgccgcg cgggagtcgc cggaccggcg
     1801 gaggacatcc gcctcgacga cccgcgcctc gtcgaatggc ggggcggttc gctggacgac
     1861 tggccgatgc caccggcgtg aggagcgcga ccggtgccgc cggcttgacg tgtgagaccg
     1921 atgccccgag acctcgatag cgcggggggc ggcgaacggt ggtgccacag gggtgacggg
     1981 gcccggcgcg gggcgtcgcg ggttgcgcac aggggcgccg aacgcgatgt cagtggcggc
     2041 aggtatcaat ggaggggtac gagcgacggc ggtgagccga cacgtgggag gggcaggatg
     2101 ccggagaccg agggacgggc atgagcgacg gcgacagcgg caacagcggc gacagtggcg
     2161 ccagcggcga cagcggcgac tacaacgacg ccagcgcggg gcacagcagg ggaagcgggt
     2221 ccccgagctg ggacgtgctg tgcagggcga tgcgctggga gaccctggcc ctgctggtgt
     2281 ttctggtggt gctcgcggcg ctggcctcct gacgccggga ggccgccgca ccaccagcac
     2341 cccggaccgg acggcaggga cggctcggta ggcccgtagg cccgtaggcc gggcagggtt
     2401 ggtccgacgc cgttctcctt cggtgggtct cggtaggggc acgggcgcaa gccgcagcaa
     2461 ttgccccgtc ccgggcggac gtttcgcagc cccgcccgtc ccgcgtgtgc acgcccgtca
     2521 gcgccccgtc cgatttccgc cagcgtggct cctcggcctg ccgcagagtg gacgtgtgaa
     2581 ggacgagaaa aggcaggtac gaggccgtgc gccgcagggg acgcccgctg aaccgcagca
     2641 ggtacagccc gcccccgccc ccgcaggcac cgggccccga caccacggaa agaacacgac
     2701 catgacgctc tacaccacac tcgacagccc gctgggcgaa ctcctgctgg tgggtgagga
     2761 atccgccacc gcgccgggcg gcacggcact cgtctcgctc tccgttccgg accagaaggg
     2821 cggggtcacc gtccaggacg gctggcagca cgcccccggg gcgttcaccg agatcaccag
     2881 ccagctgcgg tcctacttcg atggcacgct gacccgcttc agcatcgagt acgcgaacac
     2941 cgcgagcacc cggggcaccg gcttccagca gcgcgtgtgg caggcgctgg agtcgattcc
     3001 gtacgggacg accaccacct acgggcggat cgccgccgac atcggggcgg cgcgcggtgc
     3061 ggtccgtgcg gtcggaacgg cgatcggtgc caatccgctg ctcgtcgtcc ggccctgcca
     3121 ccgggtgatc gccgcgaacg gggcgctgtc gggctacgcg ggcggaccgg cgcgcaagcg
     3181 gcagctcctc ggccttgagg gggtcgtacc gaacgggagc acgccatgac cgccaccacc
     3241 acgaagaacc gcgccgggac gcgcacggcc cccggcccgg cccgccgctc cgccgcgctc
     3301 gcggcacgcg tcgcggccac cgactgggcg gcgctgggcg aggaactcga cacggtgggc
     3361 tgcgcgttga ccccgcgtct gctcaccccg gccgactgcc gcgcgctgat cgccctctac
     3421 gacgaggcag agcgcttccg gtcgaccatc gacatggcgc gccaccgctt cggttcgggc
     3481 cagtaccggt acttcgccga gccgctgccc gaaccggtcc gggccctgcg cgaggcgttc
     3541 tacccgcacc tgctgaccgt cgcccgggcc tgggcggaca agctcggcag gccggcgccc
     3601 tggccggaca ccctggagga gtggctccag atgtgccacg cggccggcca gaccaagtcc
     3661 gcgcagatcc tgctgcggta cggggcgggc gactggaacg cgctgcaccg tgacctcttc
     3721 ggcgagcggg tcttcccgct ccaggtcgtc atcggcctgg acgagcaggg caccgattac
     3781 gagggcgggg agttcctgct cgtcgaacag cggccgcgcg cccagtcccg cggcaccacc
     3841 accgtcctgc cccaggggca cggcctggtg ttcaccaccc gcgaccgccc cgtgcgctcc
     3901 gcacggggct ggtcggcagg cgccgtacgc cacggcgtga gcaccgtccg cgcgggccgg
     3961 cggcactccc tgggcctggt cttccacgac gcctgacgag gtctgacgcc tgacgaggtc
     4021 tgacgaagtc tgcgcgatgc ctgacgacgc cgcccgcgcc ggacccaggg acgcgtggcg
     4081 ctcagagcat cgagccgccc gtgacgtcga agcgctggcc ggtgatccag cgggagtcgt
     4141 ccgaggcgag gaacgcggcg acatcggcga tgtccgcggg ccggcccagg cggttgaaca
     4201 cggagatgga ggccagcgcg gcctcggcct ccggcgtcgc ccgcatcgcg gcgttcatgt
     4261 cggtggcgac gaagcccggc gcgatggtgt tcacggtgat tccgcgcgcg ccgaggtcct
     4321 tggcgagggt gagggtgagg gtgtcgaccg cgcccttgga gacgccgtac gcgatggtcg
     4381 acgggaacgc cgtggtcgtc accgccgatg agatgttgat gatgcggccg ccgtcgcgca
     4441 tccggcgcag accgtgctgg atgaggaaga acggcgcctt ggtgttgacc gcgagggtgc
     4501 ggtcgaactc ctccggcgtc acctcgtcga tgtgtcccgg caggctgatg gcggcgttgt
     4561 tgacgaggat gtcgaggccc ggctccgcgc cgcaggccgc gagcccgtcg tcgaaggcgg
     4621 cgaagagagt ctcggcgtcg cccggcaccc caagctccgc gccgattatg aaggcccgcc
     4681 cgccggcgcc tctgatggtc tccgccgtct ccttggccgc cgtgtcgttg tggccgtagt
     4741 ggacggcgac cagcgcgccg tcctgggcga ggcgttgggc gatgccccgg ccgatgcccc
     4801 ggctggaccc ggtcaccagc gcggtctttc cgttgaggtc acccatgatt atttccggcc
     4861 ttccgtgaac tgtcgcggca tgctgccgcg ttggctgtcg attgcggtgc gaggggcggc
     4921 acgcgcgtgc cgaagcaacg gaaaggttgc gtgacgcggg cggcgcgcca ggtctgtggc
     4981 gctgcgggcg aggtgtccgg tcagccgcgg gagtcccggg ctgtcagcgt cgacacgaac
     5041 accgggtggc cgtcctgcga gccggtgacc tggaccgccg tggtgccggg cagggcggac
     5101 gggacccggc gcgcctcgat cgcgcacggg gtgccgtgct cggcgtagcg gtggaatgtg
     5161 gtgccgaagg cgtacggcac gaacgtgccg ggtgcggtca gggcgtgcgc ggcctgctgt
     5221 gccgcgtcga gcagcaccat gccggggacg tggtcgttgg ccgggttcac gaccgcggcg
     5281 tgtccgggcg cgatgcgcag cagccagctg tcgggccggt cggtggggga gagcagtacg
     5341 tcgtgctcgc tggtgctgcc ggtcagccgg ggcgggacgc aggtcggcag cgggccgagg
     5401 gtgcggcggg cggtgagctg ttcgccgcgg atccggcggt agacggccgg cgaggcgacg
     5461 gtgacgtggg agtggcccgt ggccacgatc cggtccgcgg cgcgcagcgt catgtcgaag
     5521 cgggcaccgg ccagcgtgcc ggcccgcatc cgcatcttgc tgcagctgac gtcgatcgag
     5581 agcccgatgg gcccgctgcc ggcgtcgagg tgttcgggat acgtggtgta gtcgaggtcc
     5641 ccgaggatga agtggtggcc gaccgggacg ccgagttcgg tgtgcgacag cagcaacccg
     5701 gcctggcgga tggtctcgcc ggtgaggatg aggtggtgcc ggccgtcctg ggaggcgaag
     5761 tacccgtgct cctcgggcca ctgggcggtc acggagaagt gggagtcgtc ctgtcggcgc
     5821 caagatgtga ccagaatgtc atcgacaatc gggcggtgca caaattcgcg ggggacccgt
     5881 ggggtgccgc agggcttttc gatcaggcgc gtccgatgcg gtgccttggg ctgaactgtg
     5941 ccgctgacgt gcatcgtttc gctcatgact gcccccaggc atctctacgg aatgaggtcc
     6001 atcgctgtta agatacgtac catccggttt tattttcaat gccgggccgg gggaaagggg
     6061 aaataggctg gtcgagcggc gacttgagct gtagcgaaca gttgcggaac ttgaagattc
     6121 gagagacagg aggcgtcctg tggcacaaca ggaccgcgcg atccggaccc gccgagtgat
     6181 cttggaggcg gccgcgtcgg ttttcgacga gcagggctac gaccgcgcga cgatcgccga
     6241 agtcctggaa cgcgccgggg tgacgaaggg cgccctgtac ttccacttcg cctccaagga
     6301 gcagctcgcg ctcgccgtac tcgaagagca ggtcgtcgac atcgccgtcg agccgcagaa
     6361 gatcaagttg caggaattcg ttgacgccgg gcaggtgctg gcctatcgcc tgcgacggga
     6421 cccgatccag cgcggggcgg cccggttggc ggtcgagcag gggtcgaacc atcttgaccg
     6481 caagcagtcg atgatctcgt ggacgcggtt cgtcgagggg ctgctgaacg aggcgcgggc
     6541 ccgcggcgaa atcctggaga gcattgtcgt acgcgacacc gccgagctgt tcgtcggtgc
     6601 gttcgccggg ctccagatga tgtcgcacgc gatgagcaac caggccgacc tgagccaccg
     6661 gttgacggtc ttcttcgagc acaccctccc cagcatcgcg gtgcccgcgg tgctcgccaa
     6721 actcaatctg gatcccgcgc gcggggagaa attggaggcc gctctccagc ggaagatgtc
     6781 ggcggcagcc gaggccgggg agagcgtcgc tgtcggctga gtgccgggct gtacggacct
     6841 ggggggaccc gggggatttg cgtggttcag ggggttttgg gggcggtaaa cataccgcgc
     6901 acgcggtgtt gattgctgag cagggcaggg gccggcggct cgtacgagac gggtcggtca
     6961 gggggtgagg gcgccgcggt ggaccgcggc gcccctcctg tttgtggggc cgccggtgcc
     7021 ggcgggattg tcagtggcct gcgcttcact ggagttggcc ggtcccacga ggggcggctc
     7081 ggaagtcggg gccggggggg gcggcgtgag gcagatcacc gtcaaggacg tgcggcgctt
     7141 accgaaccgc ggaatagggc gccggaaaaa catgttcgcc atgcacgtat caatggcgcc
     7201 tgccgcaccc actcctgccg ccctggaggc cgcacgtatg acacagacga cgacggacca
     7261 gctgcccgga aggacggcga gcaccgccgc ggcgcccccg gtgcccgagc cgggtgcggg
     7321 cggccaccgc agagcccgcg ggggcatcgt cccggtcctg gccttctccg gcatcgtcgt
     7381 cgcggtcatg cagacgctgc tcgtgccggt catcaaggac ctgccggtgc tgctcaacac
     7441 cgcccccagc aacgccacct gggtcatgac ggccaccctg ctcgcggggc cgtctcgacg
     7501 cccatcatgg ggcggctcgg cgacctctac ggcaagcggc ggatgctgct gacgagcctc
     7561 gcgatcatgg tcgtcggctc gctgatctgc ggcgtcacca gcgatctgat cgtcatgatc
     7621 gtgggccggg cgctgcaggg ctttgccatg ggcgccatcc cgctcggcat cggcatcatg
     7681 cgtgacgagc tgccgcgcga gcggctcggc tcggccatgg gcctgatgag ctcgtcgatc
     7741 ggcgtcggtg gcgggctcgc cctgccggcc gcggcgctgg tggcccaaca cgccgactgg
     7801 cacgccctgt tcttcggcgc cgcgggtctc ggtgtgctgt cgatactgct caccctcctc
     7861 gtcgtccccg agacgtccgt acgggccgtg gggcgcttcg acgtggccgg ggccctgggg
     7921 ctgtccgccg ggctggtggc cctgctgctg cccatcacca agggcagcga ctgggggtgg
     7981 agttcgccca ccacgctcgg cctcttcggc atcgcggccc tcatcctggt gctgtggggc
     8041 gtcatggagc tgcgcatccg cgacccgctg gtcgatctgc ggaccaccgc ccgccgcgag
     8101 gtgctgctga ccaacctcgc ctcgatcacg gtcggggtgg cgttctacgc catctcgctg
     8161 gtgctgccgc agctgctgca actgccgaag tcgaccggtt acggtctcgg ccagtcgatg
     8221 gtgacggccg gtctgtgcgt ggcaccgctc ggcctgacga tgatgttcgt cgccccgctg
     8281 tacgcgcgga tcgccgcgcg gcgcggcccc aaggtctcgc tgctgctcgg catgctcgtc
     8341 atagggatcg gctacggcgc gggcatcggc ctgatgagcg ccccctggca gaccgtcatc
     8401 atcgcggtgg tcgtcggcgc cggcatcggc ctcgcgtact cctcgctgcc cgcactgatc
     8461 atcggcgctg tcgacccctc cgagaccggc gcggccaacg gcctcaacac cctcatgcgc
     8521 tcgatcggca cctccgtctc cagcgcggtg atcggcatgg tgctggccca tatgtcgcag
     8581 agcatgggac cggtcacggt ccccacgatg gccggcttcc gggtcgcctt cctcatcgcc
     8641 gccgccgcgg tcctggtcgg cgtcgtcctg gcgtcgttcc tgccgtcgca gcgcaaggtc
     8701 tcccggccga ccctggtcgc gcagagcacg gaggacacgg gagcggtgcc cgagggggag
     8761 cgccgccagg cgtcctccac ccctgccccg gctgctccgg cgccgaccgc tgtggccgag
     8821 aacgcagccg cgaccccggc tgacgtggac cccgtcgccg ggcccctcgc gcagggccgc
     8881 gccgccggat tccggggccg cgtacggtac gcctcgggcg cgccggtcgc gggcgcgagc
     8941 gtgacgctga tcgaccggca gggacggcag gcgggcatca ccacggccgg ccccgacggc
     9001 agttacgcgg tggccgcccc gaccgccggc agctacctcc tcacggccgc cgcgcccggc
     9061 cacaccccgc acgcggcctc cgccacctac cgcggcgagg gaaccccggc cgaggtggac
     9121 ctgatacttg gcggcggagg ccgcctgggc ggcaccctgc gcggtgcagg tgcgggggac
     9181 gcaccgctgg ccgacgggag cgtcgtcgtc accgacgccg gcggcgacgt ggtggcccgt
     9241 acggccacgg acgcgcacgg gcactgggaa ctcaccacgc tgccgcccgg cgagtacacc
     9301 ctggtcctca gcgcccccgg ccaccggccg cacgcccaag ccctcgaact gtccggcggt
     9361 gagcccgccc ggcacgatgc ccggctgcgg cccaccgcca ccgtccgcgg caccgtccgg
     9421 gacccgaagg ggaggccgct ggccgacgcc gccgtgaccc ttgtcgagga cggcacggtc
     9481 gccgggcaca cgctcaccgg ccaggacggc gccttcgcct tcgacgacct gggcggcagc
     9541 cactacacgc tcaccgcggc cggttatccg ccgcacgcca ccccgatatc cctcaccgcg
     9601 ggcgccgccg agaccctcga cctggacctc gaacagccga gcggcgtgcc cggctgacac
     9661 ccccggcgac tcgcccgccc gccgcccctc cgtccactgc cccaacatct cgtcgagcag
     9721 gcccgggcgc aggagcgtcg gacccgaggt gaactcgggt aagaggacgg tgagttcggg
     9781 gtggtgggcg gcgtcgagca ccgcggcacg cagcggggca ctcacgaaga ccccggcgcc
     9841 ttggccggcg cggctgcacc cccagccccg cgccggccag accgcgtacg gcccccggac
     9901 ggcgccgccc ccggccagcc cgggacgggg accaggcagc cccacccccg ccgccctgcc
     9961 acgagttgca ctttccgagg caacgcttgc gctttcgtcc ggtgcgcctt gcctcccgtc
    10021 ggcgggtact caacgaggtg gccgccaccg gcacctcgtc accggcgtgt cgcgcggtcc
    10081 ggcgtccggt cctgcgacac gccggtgtac cgaagggaga gagatgcgcg ctgtcctgcc
    10141 cgacctcgac gtgaccaaca ccgggccggc caccaccacc gagcggcgcg ccggttcctc
    10201 gtgcggctgc acgacgccgg aaccggccgg cactacctgg tggacggcgt gtaggtgcac
    10261 gacgtcaacg gcgccgacgt caaggacccg gacccgtcgg gcggcatcct cttcgccgta
    10321 cagggggcgg cgaagcccac ccgcttcgac ggggtcacga tccgaactcc ggcgtggcgc
    10381 acacccaccg caccggatct gcaacggcgc cggcatgatc tcggaccgca acaccgtccg
    10441 ctacaacgtc agcatcaacg atcgcaacac caccgcgccc tacggcgtca tttcggtcgt
    10501 ctgtgcgccc gccacccgca ccctggtgca ccacaacacc gtcaccacga acgcccccga
    10561 cacggccgtg gtcagcagca acggccccgg aggcgtcacc ttccgtgacg acgtcttcgc
    10621 cggcgccgcg gccggctccc cgcttgccga tgcacgcaac acctacgacc acaacctcaa
    10681 cctctacgac cacaacctct acgcccggat ctccccggtg cccgccaccg atgccggcgc
    10741 ggtgcggtga ccgcggttcc tggccccgta cgacgtgcgg ctgcgggccg gatcgcccgc
    10801 cctgggcgcc ggcgtaccgg tcccgggcgg cggcttccgg gacttctacg gcaatcccgt
    10861 gcccgacccg ccgaacctcg gcgccgacca aggacggggg aagtaggccg aacctcgtgg
    10921 tgccgcgtcc gcaactcggg aattatctgc gagcctgagc ggcgca
//
