LOCUS       NZ_CP019779            11354 bp    DNA     linear   CON 14-MAR-2024
DEFINITION  Streptomyces sp. MOE7 chromosome, complete genome.
ACCESSION   NZ_CP019779
VERSION     NZ_CP019779.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MOE7
  ORGANISM  Streptomyces sp. MOE7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8399509)
  AUTHORS   Elnahas,M.O., De Leon,K.B., Amin,M.A., Hussein,M.M.D., Ali,A.E. and
            Wall,J.D.
  TITLE     Complete Genome Sequencing of Streptomyces sp. Strain MOE7, Which
            Produces an Extracellular Polysaccharide with Antioxidant and
            Antitumor Activities
  JOURNAL   Genome Announc 5 (22), e00442-17 (2017)
   PUBMED   28572320
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399509)
  AUTHORS   Elnahas,M.O., De Leon,K.B., Amin,M.A., Hussein,M.M.D., Ali,A.E. and
            Wall,J.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2017) Biochemistry, University of Missouri
            Columbia, 117 Schweitzer Hall, Columbia, MO 65211, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 8.1
            Genome Coverage                   :: 180.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002090335.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 02:19:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,435
            CDSs (total)                      :: 7,344
            Genes (coding)                    :: 7,005
            CDSs (with protein)               :: 7,005
            Genes (RNA)                       :: 91
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 339
            CDSs (without protein)            :: 339
            Pseudo Genes (ambiguous residues) :: 0 of 339
            Pseudo Genes (frameshifted)       :: 230 of 339
            Pseudo Genes (incomplete)         :: 155 of 339
            Pseudo Genes (internal stop)      :: 7 of 339
            Pseudo Genes (multiple problems)  :: 47 of 339
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:50:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6726467
            Orig. end                         :: 6737821
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019779.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Judy D. Wall 117 Schweitzer
            Hall Columbia, MO USA 65202.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11354
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="20"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11354
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11354
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6354](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6354)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            377..1129
                     /gene="hemQ"
                     /locus_tag="STRMOE7_RS29545"
                     /old_locus_tag="STRMOE7_29535"
     CDS             377..1129
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984746.1"
                     /locus_tag="STRMOE7_RS29545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29535"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_033269195.1"
                     /transl_table=11
                     /translation="MTDASTPASAPQAPAAPEKAPNAGKKAKDLNEVIRYTLWSVFKLR
                     DVLPEDRSGYADEVEELFAQLAAKDVVVRGTYDVSGLRADADLMIWWHSESSDALQEAY
                     NLFRRTRLGRALAPVWSNMALHRPAEFNKSHIPAFLADEQARNYVSVYPFVRSYEWYLL
                     PDEDRRRMLADHGKMARGFPDVRANTVPSFSLGDYEWVLAFEADELYRIVDLMRHLRGS
                     EARMHVREEVPFYTGRRKPVAELVAGLA"
     gene            complement(1220..2905)
                     /locus_tag="STRMOE7_RS29550"
                     /old_locus_tag="STRMOE7_29540"
     CDS             complement(1220..2905)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (93-507). E-value:
                     3.2e-17. Score: 54.9. Matches aSDomain:
                     nrpspksdomains_STRMOE7_RS29550_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602445.1"
                     /locus_tag="STRMOE7_RS29550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29540"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_237323779.1"
                     /sec_met_domain="PF00561 (E-value: 4.3e-24, bitscore: 84.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALALYGTIGSLIVTALATAPAGGATEAPDAPPAPVAHRIGFGR
                     CAPVEHLPASVECGKLAVPMDYARPDGKKIELTVSRIRATVPGERQGSLVFNPGGPGAS
                     SLEFPLYGGLPKWSKVARAYDFVGYAPRGVGRSAPLSCQDPKEFAKVPTDSRMHPDAAF
                     KRKKVAAARAYAEGCARRAGADLPFYNSVNNARDLDMLRAALGEKKLTYMGASYGTYFG
                     AVYATLFPGHVRRMIFDSVVNPEPRQIWYRNNLDQNIAFERRWGDWLRWVAKHDDHYHL
                     GTTRQAVQQAYDRAAAKLRRDPLDGKIGPGQLQSAFLKTGYNDAFWPMRAEALSSYLHG
                     DPKALIAQALPQSSGARAEENANAVYTAVECNDGPWPRSWQTWDRDNTEVARIAPFETW
                     DNAWMNLPCAFWPDRSESAEAGVQAAARRAEHGNGQELADATGHAAQTALDGALHGPRG
                     PLDVHTEPGALPPVLLLAAERDAATPYKGAVELQHRLPGSSLVTENGAGTHGVAFNDNA
                     CVSRYAEAYLLQGKIPAHRVYCAPRAEPVPGQKAIRAQLARPGK"
     aSDomain        complement(1385..2626)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_STRMOE7_RS29550_Abhydrolase_1.1"
                     /evalue="3.20E-17"
                     /label="STRMOE7_RS29550_Abhydrolase_1.1"
                     /locus_tag="STRMOE7_RS29550"
                     /protein_end="507"
                     /protein_start="93"
                     /score="54.9"
                     /tool="antismash"
                     /translation="LVFNPGGPGASSLEFPLYGGLPKWSKVARAYDFVGYAPRGVGRSA
                     PLSCQDPKEFAKVPTDSRMHPDAAFKRKKVAAARAYAEGCARRAGADLPFYNSVNNARD
                     LDMLRAALGEKKLTYMGASYGTYFGAVYATLFPGHVRRMIFDSVVNPEPRQIWYRNNLD
                     QNIAFERRWGDWLRWVAKHDDHYHLGTTRQAVQQAYDRAAAKLRRDPLDGKIGPGQLQS
                     AFLKTGYNDAFWPMRAEALSSYLHGDPKALIAQALPQSSGARAEENANAVYTAVECNDG
                     PWPRSWQTWDRDNTEVARIAPFETWDNAWMNLPCAFWPDRSESAEAGVQAAARRAEHGN
                     GQELADATGHAAQTALDGALHGPRGPLDVHTEPGALPPVLLLAAERDAATPYKGAVELQ
                     HRLPGSSLVTENGAG"
     misc_feature    2923..2936
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.17"
                     /tool="antismash"
     misc_feature    2924..2937
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.32"
                     /tool="antismash"
     misc_feature    complement(2925..2938)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.88"
                     /tool="antismash"
     misc_feature    2964..2981
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.08"
                     /tool="antismash"
     gene            complement(2994..3812)
                     /locus_tag="STRMOE7_RS29555"
                     /old_locus_tag="STRMOE7_29545"
     CDS             complement(2994..3812)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088889.1"
                     /locus_tag="STRMOE7_RS29555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29545"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_084774716.1"
                     /transl_table=11
                     /translation="MWLLFLLVACVAAALSCAQLCRGAVAVARATGPAPLPAPAPVPDP
                     DAADGSAAGLTLYEMAYLSGGPHRLADVVLVLMAQQRRLLLAHTGWATVVDPVGLDPVE
                     QATLSAIGPDGQRRIPAIREALVADEAVRAVGDRLVAAGLALPAAARGLRNAVRQVQGA
                     TVLALLCAAAAYTTAPAGEDRGQLLIWFTLPLILTLGTLAVARFELHPYSDWATGAGEE
                     RLPGRPGFTAFSPSSPAPVAPLASLMTLALHGPGALTDPTLRAALHSSGA"
     misc_feature    3855..3869
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     gene            complement(3991..4995)
                     /locus_tag="STRMOE7_RS29560"
                     /old_locus_tag="STRMOE7_29550"
     CDS             complement(3991..4995)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055541.1"
                     /locus_tag="STRMOE7_RS29560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29550"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_107487465.1"
                     /transl_table=11
                     /translation="MFFVLWVYVGLGVSSLALVIGTIVLRLRTPSVRRADALAHDVWEA
                     AFLAGGPARVVDAAVAGMHEDGLLAVGGPGVVTVRQATARDAVEAAVLDAVARTPGGAL
                     AALRAEAMRSPAVQDVGDRLAARGLMRHPRLGRGWRLWARAHQTACGVLFVVGVVLSAS
                     GNDGIEGQTIVMILPALIVGLVVGSLCRKAFKRRITRAGSAALRSARAAHPEEAGGVWQ
                     AAGLVVALGGTALLADELLREQFEEAHRASALAAASGGGSASSGGSSSGFDSGSGSGDG
                     GSSWCGSSDGGSGSSCGGSSCGGGSSCGGGSSCGGGSSCGGGSSCGGGCGGGS"
     gene            complement(5001..6354)
                     /locus_tag="STRMOE7_RS29565"
                     /old_locus_tag="STRMOE7_29555"
                     /pseudo=""
     CDS             complement(5001..6354)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049032.1"
                     /locus_tag="STRMOE7_RS29565"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29555"
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 8.2e-98, bitscore: 325.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPEIAEDIAELPGVDWVEVVAENLCPGHLPPALQQ
                     LRERGTTVVPHGVSLGLGGADRPDRGRLTALAERAEALGAPLVTEHIAFVRAGGPLTAS
                     PVMEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALINWPGEEMTEGQFLS
                     ELVERTGVRLLIDVANLHTNHVNRGEDPAVALDELPVEAIAYVHVAGGVERDGVWHDSH
                     AHPVTQPVLDVLAALCARVTPPGVLLERDDDFPEGPELAGELDAIRTVLKEAGHAYAA"
     misc_feature    complement(5203..5205)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6480..7160)
                     /locus_tag="STRMOE7_RS29570"
                     /old_locus_tag="STRMOE7_29560"
     CDS             complement(6480..7160)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578488.1"
                     /locus_tag="STRMOE7_RS29570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29560"
                     /product="LysE family translocator"
                     /protein_id="WP_084774718.1"
                     /transl_table=11
                     /translation="MLTSLIGFTAVAALLTVSPGPDFAVVLRTALSSGRRAALCSALGI
                     AAGCFVWGLAGAVGLTAMLSASKAAYHALRVAGALYLMWLGVQALRSARRIRPAGAGSA
                     ASAPGRAAHESTVEPDSSAGPVTPLRAFRTGLLTNVLNPKVGVVYISLLPQFIPHGAPV
                     VATTLLLVAVHAALGVLWLGGIAVAVHRARAVFQRPRVRQRLDQATGGVLLALGAAVGF
                     ETAR"
     gene            complement(7289..8053)
                     /locus_tag="STRMOE7_RS29575"
                     /old_locus_tag="STRMOE7_29565"
     CDS             complement(7289..8053)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602443.1"
                     /locus_tag="STRMOE7_RS29575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29565"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_084774721.1"
                     /transl_table=11
                     /translation="MTSTETAGQPGNSSSSPFRHEETDRDAAPQFVLPLVARIERAEPP
                     SRTDALETAARAVLVLLADERSAGDGEWARAVRDWQDARIRKVVRRARGAEWRRAEALP
                     GITVGGRDAAVRVFPPVPLDGWPKELARLQVSGTELDDPLPPEAPADGLPVLWLNPELE
                     MSAGKEMAQVGHGAQLAWWELSEEERAAWRGTGFALAVRSAEPSEWAALTRSGLPVVRD
                     AGFTEIAPGSCTVVADHPALRKALPYEGPQGG"
     gene            8444..>9301
                     /locus_tag="STRMOE7_RS39430"
                     /old_locus_tag="STRMOE7_29570"
                     /pseudo=""
     CDS             8444..>9301
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018852476.1"
                     /locus_tag="STRMOE7_RS39430"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29570"
                     /product="ABC transporter ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRLDGVGRRYGVRGPWVLRDVHLEVPRGALLRVEGTNGSGKSTLL
                     RLLAGIDHPSTGRITGRPRSAYVPERFPAALPFTAVGYLTHLGRIHGLRGPEAARRAAE
                     WLERFGAAGHARTRLAELSKGTSQKVAVAQALLAEPALLVLDEAWTGLDRGAREVLDRA
                     VAERVADGGTVVFVDHDPQRLAGAADARYRVADGRLLPHEAARPTAGSVAPTGPRVVIE
                     AEGAPGAVPPAPAFAGAPGLPGGPEITPGPDGTTRLTVAAVHSDALLRTLLAARPPWHI
                     RSLTP"
     gene            9429..10097
                     /locus_tag="STRMOE7_RS39435"
                     /old_locus_tag="STRMOE7_29575"
     CDS             9429..10097
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602441.1"
                     /locus_tag="STRMOE7_RS39435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29575"
                     /product="ABC transporter"
                     /protein_id="WP_084774722.1"
                     /transl_table=11
                     /translation="MIALLRYQAALLIGSHRWLPPVILYAAFLAVGVQGGDPILDAFGW
                     AAAGLLPVAVWFTRVCVTNEPPAARSCTAAAAGPGRVHLACVLTAVGAGALLALLGAVA
                     VLLLSDPHSGDHRVAVPVLPATTAGLLAALACLLLGTALGTLCNWPVLRNPGWGIPTGL
                     TAALLLLVLGASPANAAVTGLVTGSAHGTVNTPWLPLAATVAIAAATTAAACALTSRRT
                     "
     gene            10594..10773
                     /locus_tag="STRMOE7_RS38555"
                     /old_locus_tag="STRMOE7_29580"
     CDS             10594..10773
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972872.1"
                     /locus_tag="STRMOE7_RS38555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29580"
                     /product="hypothetical protein"
                     /protein_id="WP_084776409.1"
                     /transl_table=11
                     /translation="MAPAHASAHAPAPAPRAVASPPADCLQCGEPTEYPVEQPGIVLCP
                     VCEWQEAQRSACSG"
ORIGIN
        1 acgccgaccc ggacctcttc gtcctgcgca cctccgtcgg gcgccacggc gaggaacagg
       61 acctggcccg ggacgactcc gagctggtcg ccctctccct gggcgacctg cgcgaggcgg
      121 tcgggctgac cgccgcgccg gtcgcctccc gggtcacccg ctgggacggc gggctgcccc
      181 agtaccccgt aggacacctg gagcgggtgg cccgtatccg ggccgccgtc gccgggctgc
      241 cgggtctgcg ggtgtgcggc gcgctgtacg agggcgtcgg catcccggcc tgcatcgcct
      301 ccgcccgcag ggccgccgac gacatcctcg gcaccctgcg cccggccacc gtccccggcg
      361 agtgagaatg gacgacatga cagacgcttc cactcccgct tccgcgccgc aggcccccgc
      421 ggcgcccgag aaggctccga acgccggcaa gaaggccaag gacctcaacg aggtcatccg
      481 ctacaccctg tggtcggtgt tcaagctgcg cgacgtcctc cccgaggacc gcagcggcta
      541 cgccgacgag gtcgaggagc tgttcgcgca gctcgccgcc aaggacgtgg tggtccgcgg
      601 cacgtacgac gtgtccggtc tgcgcgccga cgccgacctc atgatctggt ggcactcgga
      661 gagctcggac gccctccagg aggcgtacaa cctcttccgc cgcacccggc tcggccgcgc
      721 gctcgcgccg gtgtggtcca acatggcgct gcaccgcccc gccgagttca acaagtcgca
      781 catcccggcc ttcctggccg atgagcaggc gcgcaactac gtgagcgtct accccttcgt
      841 gcgctcctac gagtggtacc tcctccccga cgaggaccgc cgccggatgc tcgccgacca
      901 cggcaagatg gcccgcggct tcccggacgt gcgcgccaac accgtgccgt cgttctcgct
      961 cggcgactac gagtgggtgc tcgccttcga ggccgacgag ctgtaccgca tcgtcgacct
     1021 catgcgtcat ctgcgcggct ccgaggcgcg gatgcacgtc cgcgaggagg tcccgttcta
     1081 caccggccgg cgcaagccgg tggcggagct ggtggccggc ctggcctgac ctgccggcac
     1141 ggaaaacggg ccgcccccgg ggcggcccgt ttccggtcgt gcgggcccgt cgagcccggc
     1201 ggttccgttg cccggcggtc tacttcccgg gccgcgcgag ctgcgcccgg atcgccttct
     1261 ggcccggcac cggctcggcg cgcggggcgc agtacacccg gtgcgccggg atcttcccct
     1321 gcagcaggta cgcctcggcg tacctgctca cgcaggcgtt gtcgttgaag gcgacgccgt
     1381 gggtgcccgc cccgttctcg gtgaccagcg aggagccggg cagccggtgc tggagctcca
     1441 ccgcgccctt gtacggcgtc gccgcgtccc gctcggccgc cagcagcagc accggcggca
     1501 gcgcgccggg ctcggtgtgc acgtcgaggg ggccgcgcgg gccgtggagg gcaccgtcca
     1561 gggcggtctg tgcggcgtgg ccggtggcgt ccgccagctc ctgcccgttg ccgtgttccg
     1621 cccggcgggc cgccgcctgc acaccggcct cggccgactc cgaccggtcg ggccagaagg
     1681 cgcacggcag attcatccag gcgttgtccc aggtctcgaa cggggcgatg cgggcgacct
     1741 cggtgttgtc ccggtcccag gtctgccacg agcggggcca gggcccgtca ttgcactcca
     1801 cggccgtgta gaccgcattg gcgttctcct cggccctggc gccggacgac tgcggcaggg
     1861 cctgggcgat cagtgccttg gggtcgccgt gcaggtagct ggagagcgcc tcggcgcgca
     1921 tcggccagaa ggcgtcgttg tagccggtct tgaggaaggc cgactgcagc tggccggggc
     1981 cgatcttgcc gtcgagcggg tcgcggcgca gcttggcggc ggcccggtcg tacgcctgct
     2041 gcaccgcctg ccgggtcgtg ccgaggtggt agtggtcgtc gtgcttggcg acccagcgca
     2101 gccagtcgcc ccagcgccgt tcgaaggcga tgttctggtc gaggttgttg cggtaccaga
     2161 tctgccgcgg ctcggggttc accacgctgt cgaagatcat ccgccggacg tggccgggga
     2221 agagcgtcgc gtagaccgcg ccgaagtacg tcccgtacga ggcgcccatg taggtcagct
     2281 tcttctcgcc cagcgcggcc cgcagcatgt ccaggtcgcg ggcgttgttg accgagttgt
     2341 agaacggcag gtccgccccg gcccggcggg cgcaaccctc ggcgtacgcc ctcgccgcgg
     2401 ccaccttctt gcgcttgaag gccgcgtccg ggtgcatccg gctgtcggtc gggaccttgg
     2461 cgaactcctt ggggtcctgg cacgacagcg gagccgaacg gcccacgccg cgcggcgcgt
     2521 agccgacgaa gtcgtacgcg cgggccacct tgctccactt gggaaggccg ccgtagagcg
     2581 ggaactccag gctggacgcg ccgggcccgc cggggttgaa gaccagcgag ccctgccgtt
     2641 caccgggcac ggtggcgcgg atccggctca cggtgagctc gatcttcttg ccgtcgggcc
     2701 gcgcgtagtc catggggacg gccagtttgc cgcactcgac ggacgccggc agatgctcca
     2761 ccggagcgca ccgtccgaaa ccgatccggt gcgccacggg cgccgggggc gcatcgggcg
     2821 cctccgtggc accgcccgcc ggggcggtgg cgagggcggt cacgatcagc gacccgatgg
     2881 tgccgtacaa cgcaagtgct ctcactggcc gtcgtccctt cgtgttgtac atacaaaagg
     2941 gatatttggg tcggtggttg gcgatgtaaa gcacctgatg ccggtgtcgg acgctatgcc
     3001 ccggatgagt ggagcgccgc gcgcagggtg ggatcggtga gggctccggg gccatggagg
     3061 gcaagagtca tcaaggaggc gaggggtgcg acaggggcgg gggagctggg ggagaacgcg
     3121 gtaaagccag gtctgccggg cagtcgctct tcgcccgcgc cggtcgccca gtcgctgtac
     3181 ggatggagct cgaagcgcgc caccgccagc gtccccagcg tgaggatcag cggaagcgtg
     3241 aaccagatca gcagctgtcc ccggtcctcg ccggccggcg ccgtcgtata cgccgccgcg
     3301 gcgcagagca gcgccagcac cgtcgcgccc tgaacctggc ggacggcgtt gcgcagccca
     3361 cgggccgcgg cgggcaacgc gagcccggcg gccaccagcc ggtcgccgac cgcccgcacc
     3421 gcctcgtcgg ccaccagggc ctcccgtatc gccggtatcc gccgctgccc gtccggccct
     3481 atggcgctga gggtggcctg ctcgacgggg tccaggccga ccggatcgac gacggtggcc
     3541 cagccggtgt gggcgagcag cagccgccgc tgctgcgcca tgagcaccag caccacatcg
     3601 gccagccggt gcgggccgcc ggagagatac gccatctcat acagcgtcag acctgccgcg
     3661 gagccgtccg cggcgtccgg atccgggacg ggggcgggcg ccggcagcgg ggccggtccg
     3721 gtggcgcggg cgacggccac ggcaccacga cagagctgcg cacagctcag cgccgccgcc
     3781 acgcaggcga cgaggaggaa gaggagccac atggcagctt tttacgggat gagacatgac
     3841 aaccgccatt cggtttctca acatgcgggc gcgcaatgga cggggccgtc ggggcgcggg
     3901 gtgcgggcct gctgctggac cggcccgggg aggggctgtt gcccccgggc cggccccggc
     3961 gttcgctgcc gtcgtagccg gaaaccgccc tcagctgccg ccgccgcagc cgcctccgca
     4021 gctcgatccg ccgccgcagg acgatccacc gccgcaggac gagccgcccc cgcacgacga
     4081 tccaccgccg caggacgaac cgccgcagct cgaaccggac ccgccgtccg acgagccgca
     4141 ccaggaagag cccccgtcgc ccgacccgct accggagtcg aagccggacg acgagccgcc
     4201 ggacgatgcg gacccgccac ccgacgcggc ggccagcgcg ctcgcccggt gcgcctcctc
     4261 gaactgctcc cgcagcagct cgtccgcgag cagtgcggtg ccgccgagcg ccaccaccag
     4321 cccggcggcc tgccacaccc cgcccgcctc ctcggggtgg gcggcccgcg ccgagcgcag
     4381 cgcggcggaa cccgccctgg tgatccggcg cttgaacgct ttccggcaca gcgatcccac
     4441 caccagcccc acgatcagcg ccggcaggat catgacgatc gtctgcccct cgatcccgtc
     4501 gttgccggac gccgacagca ccacgccgac gacgaagagc accccgcacg ccgtctggtg
     4561 cgcccgcgcc cacaggcgcc agccccggcc gagccgcggg tgacgcatca ggccccgcgc
     4621 cgccagccgg tcgccgacgt cctggacggc cgggctgcgc atcgcctcgg cgcgcagcgc
     4681 ggccagcgcc ccaccgggcg tgcgggcgac cgcgtccagc acggccgcct cgaccgcgtc
     4741 ccgggcggtg gcctgacgta cggtcaccac gcccggaccg cccaccgcca gcaggccgtc
     4801 ctcgtgcatc ccggcgaccg ccgcgtcgac cacccgcgcc gggcccccgg cgaggaaggc
     4861 cgcctcccag acgtcgtgcg ccagcgcgtc cgcccggcgg accgacggcg tccgcagccg
     4921 gagcacgatc gtgccgatca cgagcgccag cgacgagacg ccgagcccca catacaccca
     4981 caggacgaag aacatcccgg tcacctccgc cgcagaacgt ggtgcagggc accgtccagg
     5041 ccgtcacgca gggcgcgcgc cagacgggtg accggccggg ccgacggcgg cctggggccc
     5101 gcccggtccc gccaccagtg cgtcaactgc tgccgtgcgg ccgcgtccgc gggcaggtcc
     5161 tgggcgagca ggtgcgccgc gaagtccagg gcgtcctggc ggtaaccacc gctcatcgga
     5221 cggcaccggg catagccgag gaacgccggc cggaagccag cgccgaggat ctccggcagc
     5281 tcgggagcga ccttggcgac caccgaggcc cgcttggcgg tcagcgcccg gctctgcacc
     5341 tgaagccggg tgtggtcgaa gccctcgggc gccggcgtcc cggcgaccag cgcggacagc
     5401 agcgcggcct gcgccagggc gagccgctgg cgggcggcgt cggtgccggt gccggtaccg
     5461 gggtctgcgc cggaagcggg tatcggggag gagggtgcct cctgtacgga ctcaagccgc
     5521 gtacgcatga ccggcctcct tcagcaccgt gcggatcgcg tccagttcac cggccagctc
     5581 cgggccctcc gggaagtcgt cgtcccgctc cagcagcacc ccgggcgggg tgacgcgggc
     5641 acacagcgcg gcgagcacgt ccagcaccgg ctgggtcacc gggtgggcgt ggctgtcgtg
     5701 ccacaccccg tcccgctcca ccccgcccgc gacatggacg tacgcgatgg cctcgaccgg
     5761 cagctcgtcc agcgcgacgg ccgggtcctc gccgcggttg acgtggttgg tgtggaggtt
     5821 ggcgacatcg atgagcagcc gtacgccggt ccgctcgacc agctcggaca ggaactgccc
     5881 ctcggtcatc tcctcgccgg gccagttgat cagggcggcg atgttctcca gggccagcgg
     5941 caccggcagc gcgtcctggg cgatccgtac gttctcgcag agcacgtcca gcgcgtcacg
     6001 ggtccgcggt acgggcagca gatgcccggc ctccatcacc ggcgaggcgg tgagcggccc
     6061 gccggcccgc acgaacgcga tgtgctcggt gaccagcggc gcgcccagtg cctcggcgcg
     6121 ctccgcgagg gcggtcagcc ggccgcggtc cggacggtcc gcgccgccga ggccgagcga
     6181 gaccccgtgc ggaacgacgg tcgtgccccg ctcacgcaac tgctgcaggg cgggcgggag
     6241 atggcccggg cagaggttct ccgccaccac ctcgacccag tccacccccg gcagctccgc
     6301 gatgtcttcg gcgatttccg gccgccagcc gattcccgtc cccaggcgct ccatgcctcc
     6361 ccctctcgct ctgtggtcgg ggtactcatc gccccgtcgc gacggcgtga accctgcacc
     6421 ggcctggtcc agaggttgat ttgagcttcc cccgcgattc cgcccaccgc cgcgggccgt
     6481 cagcgggcgg tctcgaatcc caccgcggcg cccagcgcga gcagcactcc gccggtggcc
     6541 tgatccagcc gctgccggac cctcgggcgc tggaagacgg cccgcgcacg gtgcaccgcg
     6601 acggcgatcc cgcccagcca cagcacaccc agcgcggcat gcacggcgac cagcaggagc
     6661 gtggtcgcga cgactggcgc gccgtgcggg atgaactgcg gcagcagcga gatgtagacc
     6721 acgccgacct tggggttgag cacgttggtc agcaggccgg tgcggaacgc gcgcagcggc
     6781 gtgaccgggc cggcggacga gtccggttcc acggtgctct cgtgggcggc gcgaccgggg
     6841 gccgaggcgg cgctgccggc gcccgcgggg cggatccgcc gggcgctgcg caacgcctga
     6901 accccgagcc acatcagata cagcgcaccg gccacccgca gggcgtggta cgccgccttc
     6961 gacgccgaca gcatcgccgt cagcccgacc gccccggcca gcccccagac gaagcacccg
     7021 gcggctatgc cgagcgcact gcacagcgcg gcgcgccgtc ccgagctgag cgcggtccgc
     7081 agcaccacgg cgaaatcggg gccgggggag acggtcagca gggcggccac ggcggtgaag
     7141 ccgatgagcg aggtgagcat ggcgcaacgg tatgcgcagc ggcgagcacc gcggcaagcg
     7201 cctcgggacc aggatgagcc gggcgcgggt cccggacggg ggagtggaca cgccgcgccg
     7261 ggtccgtcgc tcccgggccg ggcgccgctc agccgccctg cggcccctcg tacggcagtg
     7321 ccttgcgcag cgcggggtgg tccgcgacga ccgtgcacga ccccggtgcg atctcggtga
     7381 accccgcgtc ccgcaccacg ggcagcccgc tgcgggtcag cgccgcccac tccgaaggct
     7441 ccgccgaacg gaccgccagc gcgaaacccg tgccgcgcca ggccgcccgt tcctcctcgg
     7501 acagctccca ccacgccagc tgcgcaccgt ggccgacctg cgccatctcc ttgcccgcgg
     7561 acatctccag ctccggattg agccacagca ccggaagccc gtcggcgggc gcctcgggcg
     7621 gcaggggatc gtccagctcc gtgcccgaga cctggagccg cgccagctcc ttcggccagc
     7681 cgtccagcgg caccgggggg aagacccgca ccgcggcgtc gcggccgccg accgtgatcc
     7741 ccgggagcgc ctcggcccgc cgccactccg cgccgcgcgc ccgccgcacc accttccgta
     7801 tccgggcgtc ctgccagtcc cgtaccgccc gggcccactc accgtcaccg gccgaccgct
     7861 cgtccgccag cagcaccagc accgcccgcg cggccgtctc cagcgcatcc gtacgcgacg
     7921 gcggctcggc ccgctcgatg cgcgcgacga gcggcagcac gaactggggc gccgcgtccc
     7981 ggtcggtctc ttcgtgccgg aacgggctgc tggagctgtt gccgggctgc ccggcggtct
     8041 cggtgctggt cacccgccca gtttgccagc cgccaccgac aacgccgggc cgggaccggt
     8101 aacggcccct ggccgccggc ttgccccttc cgcccgtccg gtggctcctt ccgcccggcg
     8161 ccttccgccc ggccggtgac tcccgtccgc ccggcgcctt ccgtcccgta ggcgccttcc
     8221 gtgcggctcg tgagcaactg cgcgctcctg ccgtggggtt ggtgtggtgg tcgtgcgatg
     8281 cgcgctccca ccgcgtgccc gtgcggggat gtcgcagcac gcgtgccccg ggcgggcgcc
     8341 gccccgcctt cccggttacc gtcagcgcct ggacgccagg agacgccggg ggcgccggga
     8401 gacgggctgg ggaggtgccg gagtgccgga ggtggcgcag ggcatgaggc tggacggggt
     8461 ggggcggcgc tacggggtgc gcggcccctg ggtgctgcgg gacgtgcacc tggaggtgcc
     8521 ccgaggggcg ctgctgcgcg tcgaggggac caacggcagc gggaagtcca ccctgctgcg
     8581 gctgctcgcc ggcatcgacc accccagcac cggccgcatc accggacgcc cgcgcagcgc
     8641 gtacgtcccc gaacgcttcc cggcggcgct gccgttcacc gccgtcggct acctcaccca
     8701 cctcggtcgc atccacggtc tgcgcggtcc cgaagccgcc cgccgcgccg ccgagtggct
     8761 cgaacgcttc ggcgcggccg gacacgcccg tacgcggctg gccgagctgt cgaagggcac
     8821 cagccagaag gtcgccgtcg cgcaggccct gctcgccgag cccgcgctcc tcgtgctgga
     8881 cgaggcatgg accgggctgg accgcggtgc gcgcgaggtc ctggaccggg cggtcgccga
     8941 acgggtcgcg gacggcggca ccgtcgtctt cgtcgaccac gatccgcagc ggctggccgg
     9001 cgcggccgac gcccgctacc gggtggcgga cgggcggctg ctgccccacg aggcggcccg
     9061 gccgacagcc ggatccgtgg ccccgaccgg cccccgcgtg gtcatcgagg ccgagggtgc
     9121 cccgggcgcc gtaccgcccg cgcccgcctt cgccggagcg ccggggctgc ccggcggccc
     9181 ggagatcact ccgggaccgg acggtacgac ccggctgacc gtcgcggccg tccactccga
     9241 cgcgctgctg cgcaccctgc tcgcggcccg cccgccctgg cacatccgct cactcacccc
     9301 tgccccacga cacactccgc ccgctccgaa gcccgctccg acgccgggct cccaccccga
     9361 tcccgacccc cactccgccc ccaccccgac ccctgttccg actccgactc cgacgaggcg
     9421 ccgcttccgt gatcgctctt ctccgctacc aggcggcgct gctcatcggc tcgcaccgct
     9481 ggctgccgcc ggtcatcctc tacgcggcgt tcctcgccgt cggcgtccag ggcggcgacc
     9541 cgatcctcga cgcgttcggc tgggccgcgg ccggcttgct cccggtcgcc gtatggttca
     9601 cccgggtctg cgtcaccaac gagccaccgg ccgcccgcag ttgtaccgct gccgccgccg
     9661 ggccgggccg ggtgcacctc gcgtgcgtgc tgaccgcggt cggtgccggg gcgctgctcg
     9721 cgctcttggg ggcggtggcg gtgctcctgc tctccgaccc gcactccggt gaccaccggg
     9781 tggccgtccc ggtcctgccc gcgaccaccg cggggctgct cgccgcgctg gcctgcctgc
     9841 tgctgggcac cgcgctcggc acgttgtgca actggccggt cctgcgcaac ccgggctggg
     9901 gcatccccac gggactgacc gccgcgctgc tgctgctcgt cctcggtgcg tcgccggcca
     9961 acgccgccgt cacgggcctg gtcaccggct ccgcccacgg caccgtcaac acgccctggc
    10021 tcccgctcgc cgcgaccgtc gcgatcgccg ccgccacgac ggccgccgcg tgcgccctca
    10081 cctcccgccg cacctgaacg cccgtcctct gcctccgtac ttgcccgccc cgcgcgtgtg
    10141 tgcacggcct cttgccgccg tcttccgctg cggcccttct ccggggcagc cgtgcttccc
    10201 cgccccagtc ctgactcatc cgagcgtcct tcgccccctc ccgccctcca caggcgcttc
    10261 ccccgccccc tccccctctc ccccttcggg gagggggcgg ggggtgggtt cgggggtggg
    10321 gggaggcatc ggcgtacgct cgacaccatg cccggcaccc aggcccacgc cacccccagc
    10381 cccgcacccc acccgcacac gggccccacg gagcccacgg cccccaccga gtgctacgcc
    10441 ggccacaccg cgacgaacac cgggacgaac accgccacga acgccgcgac gaccggcaac
    10501 gacgccctcg ccaccgcgac gaacgccgcg acgaccggca acgacgccct cgccaccgcg
    10561 gcggacgccg gcgtcacaca ccgcccgtca ggcatcgctc ccgcccatgc ctccgcccat
    10621 gcccccgccc ccgctccccg tgccgtcgcc tccccgccgg ccgactgcct tcagtgcggg
    10681 gagcccacgg agtacccggt cgagcagccc ggcatcgtgc tctgccccgt gtgcgaatgg
    10741 caggaggccc agcgctccgc ctgctccggc tgacgcccac cgacggcccc ccactgcccc
    10801 gcgtcacgtc accgcctcaa tccccccaag gccccgtcac tgcgaacgtc gtgcccgggg
    10861 tgtagcagtt gacgtacatg gtgcggtggt cgggagcgaa cgtcacgccc gcgaactcgc
    10921 cccattccgg cttctccggg gtgccgatgt tctgccggcc gcgtgccatc gcgtagacct
    10981 cgccgcggcg cgtgaggccg aagacatgct gggcgccgtc gccgtcctcg cagaccatca
    11041 ggccgccgct gggggcgagg cagatgttgt cgggcgactc gccgggcagt tggaggtcgc
    11101 tgtccgggcc gaagaccacg acctgggtga gccggtgccg ctgggggtcg tacttccaga
    11161 cctggccgta gtggtcggcc gccgagccgg cggcgcggtg cgcgaagctg gagacgaagt
    11221 agacgcagga gccgccccag tagcagccct ccagcttctg ggcgtgggtg atgccgccgc
    11281 gcccgaagtc ctggaagcgg atgggggtgt ggcgggccag cgggtcgggg acgtcgaccc
    11341 aggtgacgtc gtcg
//
