LOCUS       NZ_CP019779            10903 bp    DNA     linear   CON 14-MAR-2024
DEFINITION  Streptomyces sp. MOE7 chromosome, complete genome.
ACCESSION   NZ_CP019779
VERSION     NZ_CP019779.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MOE7
  ORGANISM  Streptomyces sp. MOE7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8399509)
  AUTHORS   Elnahas,M.O., De Leon,K.B., Amin,M.A., Hussein,M.M.D., Ali,A.E. and
            Wall,J.D.
  TITLE     Complete Genome Sequencing of Streptomyces sp. Strain MOE7, Which
            Produces an Extracellular Polysaccharide with Antioxidant and
            Antitumor Activities
  JOURNAL   Genome Announc 5 (22), e00442-17 (2017)
   PUBMED   28572320
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399509)
  AUTHORS   Elnahas,M.O., De Leon,K.B., Amin,M.A., Hussein,M.M.D., Ali,A.E. and
            Wall,J.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2017) Biochemistry, University of Missouri
            Columbia, 117 Schweitzer Hall, Columbia, MO 65211, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 8.1
            Genome Coverage                   :: 180.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002090335.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 02:19:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,435
            CDSs (total)                      :: 7,344
            Genes (coding)                    :: 7,005
            CDSs (with protein)               :: 7,005
            Genes (RNA)                       :: 91
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 339
            CDSs (without protein)            :: 339
            Pseudo Genes (ambiguous residues) :: 0 of 339
            Pseudo Genes (frameshifted)       :: 230 of 339
            Pseudo Genes (incomplete)         :: 155 of 339
            Pseudo Genes (internal stop)      :: 7 of 339
            Pseudo Genes (multiple problems)  :: 47 of 339
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:50:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6657693
            Orig. end                         :: 6668596
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019779.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Judy D. Wall 117 Schweitzer
            Hall Columbia, MO USA 65202.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10903
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="19"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10903
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10903
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5903](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5903)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(1074..1550)
                     /locus_tag="STRMOE7_RS29280"
                     /old_locus_tag="STRMOE7_29270"
     CDS             complement(1074..1550)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013000125.1"
                     /locus_tag="STRMOE7_RS29280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29270"
                     /product="ATP-binding protein"
                     /protein_id="WP_084774616.1"
                     /transl_table=11
                     /translation="MQEPPAPTTWHIALPYGATAVPVARAMVRTALQDLRATADRTTAQ
                     LLTAELVANALKHARAGDSIELVVERGPAGCLVEVHDGDPALVEGLGAPAVVEGLGEPL
                     GLSSGGGHAAAPGAAGGRGLLLIRSLSSASGCRATAGGKAVWFTLPEQRRTPRH"
     misc_feature    complement(1676..1691)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.38"
                     /tool="antismash"
     gene            1764..2591
                     /locus_tag="STRMOE7_RS29285"
                     /old_locus_tag="STRMOE7_29275"
     CDS             1764..2591
                     /NRPS_PKS="Domain: ECH (19-267). E-value: 2.4e-50. Score:
                     163.1. Matches aSDomain:
                     nrpspksdomains_STRMOE7_RS29285_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982203.1"
                     /locus_tag="STRMOE7_RS29285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29275"
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="WP_033269163.1"
                     /transl_table=11
                     /translation="MSPFAGSAHRTDPWQHIRVTRQDGVATVTLARPEKLNALTFEAYA
                     DLRDLLAELSRERAVRALVLAGEGRGFCSGGDVEEIIGATLGMDTGQLLDFNRMTGQVV
                     RALRECPFPVIAAVHGVAAGAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYL
                     LPRVIGLGHATRLLMLGDTVRAPEAERLGLISELAEEGRADEAAAALARRLAEGPALAH
                     AQTKALLTAELDMPLAAAVEMDAATQALLMNSADYAEFHAAFTEKRTPKWQGK"
     aSDomain        1821..2564
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_STRMOE7_RS29285_ECH.1"
                     /evalue="2.40E-50"
                     /label="STRMOE7_RS29285_ECH.1"
                     /locus_tag="STRMOE7_RS29285"
                     /protein_end="267"
                     /protein_start="19"
                     /score="163.1"
                     /tool="antismash"
                     /translation="TRQDGVATVTLARPEKLNALTFEAYADLRDLLAELSRERAVRALV
                     LAGEGRGFCSGGDVEEIIGATLGMDTGQLLDFNRMTGQVVRALRECPFPVIAAVHGVAA
                     GAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYLLPRVIGLGHATRLLMLGDT
                     VRAPEAERLGLISELAEEGRADEAAAALARRLAEGPALAHAQTKALLTAELDMPLAAAV
                     EMDAATQALLMNSADYAEFHAAFTEK"
     gene            2594..4930
                     /locus_tag="STRMOE7_RS29290"
                     /old_locus_tag="STRMOE7_29280"
     CDS             2594..4930
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088826.1"
                     /locus_tag="STRMOE7_RS29290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29280"
                     /product="oxidoreductase"
                     /protein_id="WP_084774618.1"
                     /transl_table=11
                     /translation="MRVAVIGGGPGGLYAAALLKRLDPTREITVWERNAPDDTFGFGVV
                     LSDETLGGIEHADPEVYRALQREFVRWDDIDIVHRGHTLTSGGHGFAALGRRRLLGILH
                     QRCRDLGIELRFRTEAPPAPELARDHDLVIAADGVHSATRTAHADAFGPRLTTHRCRYI
                     WLAADFALDAFRFEIAETAHGVVQLHAYPYSRPSPDPDPRPGPGATTAPAGPQHPGGAS
                     TVIVEMREEVWRAAGLDRADERESAEWCAKVFTDALSGRALRSNNSSWIAFRTVVNERW
                     SYGNTVLLGDAAHTAHFSIGSGTKLAVEDALALAACLQEQPDTAAALTAYEAERRPVVA
                     STQRAARASLAWFEELATYVDQPPRQFAFNLLTRSRRVTHDNLRLRDPGFVASVEDEAA
                     VPAGTPPMFTPFRLRGLTLRNRVVVSPMDMYSAQDGVPGDFHLVHLGARALGGAGLVMT
                     EMVCVSERGRITPGCAGLYTPQQAAAWRRVTDFVHAQAPGAAIGVQLGHAGRKGSTKLM
                     WDGIDEPLDDGNWPLVAPSPLPYKPGSQRPQELTAGELAEIREQFAAAARRAVDCGFDL
                     LELHCAHGYLLSGFLSPLTNQRTDAYGGSLSRRLRFPLEVFDTVRALWPDDKPMTVRIS
                     ATDWADGGTSAEDAVAIAAAFAEHGADAIDVSTGQVVAEERPDFGRSYQTPFADRIRNS
                     LGVPVIAVGAISSWDDVNSLVLAGRADLCALARPHLYDPHWTLHAAAEQGYAGQGAPWP
                     LPYQAGSRTPPTGRTDAPKPRLTLR"
     gene            complement(5001..5903)
                     /locus_tag="STRMOE7_RS29295"
                     /old_locus_tag="STRMOE7_29285"
     CDS             complement(5001..5903)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088827.1"
                     /locus_tag="STRMOE7_RS29295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29285"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_237323775.1"
                     /sec_met_domain="AfsA (E-value: 7.6e-24, bitscore: 82.3,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTVPREYVHRTAVSEVFLTNWRRGAADSWIVSAQWPRAHSFYG
                     PVGGLHDPLLLIETMRQAGILLSHVAHRVPLDHPIIWQRVRYDLTPQALHAADEPAEVE
                     LHITDHDVIRRGKRLISARQHFRIRCDGADLATASLDYSCHSPAVYRRLRGKYSDLTLA
                     NARRLPLPEPVTPQLVGRDRERDVVLSPTNRPDRWQLRVDTSHPVLFDHPVDHAPGMLM
                     IEAARQAAQAATPEPTLPTAMECDFERYAELDAPCWVQARAADEPLTAVAGGGRQIEVA
                     VEQHGKPVIAARITSVPTP"
     misc_feature    complement(5706..5708)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(5739..5741)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(6083..6099)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.66"
                     /tool="antismash"
     misc_feature    complement(6085..6100)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.79"
                     /tool="antismash"
     misc_feature    6127..6139
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     gene            complement(6136..6753)
                     /locus_tag="STRMOE7_RS29300"
                     /old_locus_tag="STRMOE7_29290"
     CDS             complement(6136..6753)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602525.1"
                     /locus_tag="STRMOE7_RS29300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29290"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_033269160.1"
                     /transl_table=11
                     /translation="MVKQERAVRTRRAVLEAAAHVIGTRGYQAATMAEIIQRAGVTKGA
                     VYFHFTSKDALARAVITEQTDPFLPQVSESRLQDAIDFTHQVALALRSDPLLQAGTRIA
                     VETTFSDEPLVPYQAWTDIITTMFSEARDNGELLPGVAPDRAAEFFVAAYMGVQLFSRA
                     ATNRADLPERVTALWKHTLPGLASPGAMSHLDPHGRAVQVSV"
     misc_feature    complement(6343..6345)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6920..7948
                     /locus_tag="STRMOE7_RS29305"
                     /old_locus_tag="STRMOE7_29295"
     CDS             6920..7948
                     /NRPS_PKS="Domain: PKS_ER (33-340). E-value: 3.4e-19.
                     Score: 61.0. Matches aSDomain:
                     nrpspksdomains_STRMOE7_RS29305_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088829.1"
                     /locus_tag="STRMOE7_RS29305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29295"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_084774623.1"
                     /sec_met_domain="ADH_zinc_N (E-value: 3.2e-17, bitscore:
                     61.5, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPTAVPDVPAMQREVRLAARTEGELTARHFTLAEVPVPEPGPGQ
                     VLVRTRVMAVTAAMRTQMTGAPLPMPSFVPGQALWGSAVGEVVAAPGGGFAPGEIVHHP
                     YGWREFAVVDAERLRRLAPDALPTPAAHLSQGATAWGALTRAVEVRPGDTVFVTGAAGG
                     VGSLAGQLARRLGAGRVVGSTGSERKAARLRAELGYDDTIVRGAGPIDRQLRRAVPDGI
                     DVLLDTVGGEQLTAALASARPDARFALVGALATQLGGDGGAVAPVELDAGLIITRRVAL
                     RGFGLYAHPDLPQEWTKEFGQGLRDGSLVFPHALLKGIEQAPRALCELTQGRHIGAVLV
                     EL"
     aSDomain        7019..7939
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_STRMOE7_RS29305_PKS_ER.1"
                     /evalue="3.40E-19"
                     /label="STRMOE7_RS29305_PKS_ER.1"
                     /locus_tag="STRMOE7_RS29305"
                     /protein_end="340"
                     /protein_start="33"
                     /score="61.0"
                     /tool="antismash"
                     /translation="AEVPVPEPGPGQVLVRTRVMAVTAAMRTQMTGAPLPMPSFVPGQA
                     LWGSAVGEVVAAPGGGFAPGEIVHHPYGWREFAVVDAERLRRLAPDALPTPAAHLSQGA
                     TAWGALTRAVEVRPGDTVFVTGAAGGVGSLAGQLARRLGAGRVVGSTGSERKAARLRAE
                     LGYDDTIVRGAGPIDRQLRRAVPDGIDVLLDTVGGEQLTAALASARPDARFALVGALAT
                     QLGGDGGAVAPVELDAGLIITRRVALRGFGLYAHPDLPQEWTKEFGQGLRDGSLVFPHA
                     LLKGIEQAPRALCELTQGRHIGAVLV"
     CDS_motif       7373..7435
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_STRMOE7_RS29305_0001"
                     /evalue="1.50E-03"
                     /label="PKSI-ER_m5"
                     /locus_tag="STRMOE7_RS29305"
                     /protein_end="172"
                     /protein_start="151"
                     /score="11.3"
                     /tool="antismash"
                     /translation="GDTVFVTGAAGGVGSLAGQLA"
     gene            complement(7996..8637)
                     /locus_tag="STRMOE7_RS29310"
                     /old_locus_tag="STRMOE7_29300"
     CDS             complement(7996..8637)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088830.1"
                     /locus_tag="STRMOE7_RS29310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29300"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_084774626.1"
                     /transl_table=11
                     /translation="MPTQHRAIQSRQALIRSAAETFLEKGVPAAGMVEISRRARLSKGA
                     LYFHFTSKDDLTLAVRDAALATLQEIEDAFTRSPQPLTTAVQDFAVDLFERVESDAVLR
                     AGLRLRPETAPGLGIYALEQRWYALFLDKAVTCGTGGIGRPAADAPDAEPRRTAQLLTS
                     IVVGLLHLGGEDGTWWDKEAVVGLWSLLPAAPGRIAAAGIPTPDRLPAAG"
     misc_feature    8848..8864
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.33"
                     /tool="antismash"
     misc_feature    complement(8850..8862)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 20.49"
                     /tool="antismash"
     misc_feature    8885..8899
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     misc_feature    8911..8925
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.04"
                     /tool="antismash"
     gene            9090..9881
                     /locus_tag="STRMOE7_RS29315"
                     /old_locus_tag="STRMOE7_29305"
     CDS             9090..9881
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574418.1"
                     /locus_tag="STRMOE7_RS29315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29305"
                     /product="HAD family hydrolase"
                     /protein_id="WP_084774630.1"
                     /sec_met_domain="HAD_2 (E-value: 3.3e-28, bitscore: 97.7,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVATEVGERPARGARGATGGETIRAAVFDLDGTLANTLPAISKL
                     LVKVASEQGCSVTARQAATAIGKPPGPAFGRLLGRPEDDERVQAAISRYRELFSYEVLC
                     QGPQLLFPGVTAGLSALRAHGVELAVATSKTTRSAEALLDVMGIRDLVGPVIGQDMVRR
                     GKPHPEMVLRAAGRLGVAPWQSAYVGDTVGDMRMAVTARMEPVAVTYGVGTFGELAAVA
                     GTRMCSSFKDVVSVIAAARPRAAAGAPHRPAVAVGGPARRR"
     gene            complement(9893..10744)
                     /locus_tag="STRMOE7_RS29320"
                     /old_locus_tag="STRMOE7_29310"
     CDS             complement(9893..10744)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536997.1"
                     /locus_tag="STRMOE7_RS29320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_29310"
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="WP_084774632.1"
                     /transl_table=11
                     /translation="MTDQQTQRTPRSLIVTFYGAYGRGEPGATGPAAAPLPVAALIRLL
                     GVLGVDPPSVRSAVSRLKRRGLLVPGRTEAGAAAYGLSDAARQLLEDGDRRIFGRPAAR
                     LSDGWVLAVFSVPEEERHKRHLLRSRLARLGFGTAAPGVWIAPAQLYEETRHTLERLQL
                     DPYVDLFTGTHAGFAPTADAVARWWDLDAVAAQHRAFLAEQEPVLRRWSRRRTVPPEAA
                     YHDYLLALDAWRRLPYADPGLPSPLLPEGWPGGRAAEVFGRLHGTLQDAGARYVREVTA
                     GP"
ORIGIN
        1 gccggctgat cgacctcgcg gccgagctga aagcggccaa gcgcgccggt acggaggtcc
       61 ctcgcctgca gggccggaac atcgccctga tcttcgagaa gacctcgacc aggacccgct
      121 gctccttcga ggtcgcggcc gccgaccagg gcgcctcgac cacctacctc gacccgtccg
      181 gctcgcagat cgggcacaag gagtcggtca aggacaccgc ccgggtgctc ggccggatgt
      241 tcgacggcat cgagttccgc ggcagccgcc agtccgacgt cgaggatctg gccgcgtacg
      301 ccggtgtgcc ggtcttcaac gggctgaccg atgactggca cccgacccag atgctcgccg
      361 acgtgctcac catggtcgag cacggccggg gccgccccct ggaggagacc gccttcgcct
      421 acctcggcga cgcccgcaac aacatgggca actcctacct cgtcaccggc gccctgctcg
      481 gcatggacgt acggatcgtg gcgcccgagc agctgtggcc cgaggaggcg gtcgtcgccg
      541 cggcgcggaa gctcgccgac gccagcgggg cccggatcac cctcaccgag gacgtcaccg
      601 agggtgtgcg tggcgcggac ttcgtcgcca ccgacgtgtg ggtctccatg ggggagccca
      661 aggaggtgtg ggacgagcgg atcgccctgc tcgccccgta cgcggtcacg atggacgtac
      721 tgcgggccac cggaaagccg gaggtgaagt tcctgcactg cctgcccgcg taccacgacc
      781 tgggcacggc ggtcggccgg gagatccacg cccggcacgg gctgtcgtcg ctggaggtca
      841 ccgacgaggt cttcgagtcc gcgcactccg tcgtcttcga cgaggcggag aaccggatgc
      901 acacgatcaa ggcggtgctg gtcgcgacgc tggcccgata ggggacgtgc gtctggccct
      961 tcccggcctg accggtctgc ccggtcctcg gcatcggctg ctgcgtccac ctgatccacg
     1021 ggaccggcct cggcaggcgc ggcagccgcc ggccgcgccc gggcgggccg gcgtcagtgc
     1081 cggggcgtcc gccgctgctc gggaagggtg aaccagaccg ccttgccgcc cgccgtcgcc
     1141 cggcagccgg acgccgagct gagggagcgg atcagcagca gaccgcggcc gccggccgca
     1201 cccggcgcgg cggcgtggcc cccgccggag gacaggccga gcggctcgcc gagcccctcg
     1261 acgaccgccg gagccccgag cccctcgacg agcgccggat cgccgtcgtg cacctccacc
     1321 aggcagcccg ccggcccccg ctccaccacc agctcgatcg agtcgcccgc gcgggcgtgc
     1381 ttgagcgcgt tggcgacgag ttcggcggtc agcagctgag cggtggtccg gtcggcggtg
     1441 gcccgcaggt cctgcagcgc cgttctgacc atggcacgcg ccacgggcac cgcggtggca
     1501 ccgtacggca gcgcgatgtg ccaggtcgtg ggggcgggcg gctcctgcat gccctccacg
     1561 gtaggaagcg cggccggccc ggtttcagag ccgagcggcc ggcttcggaa ggacagaggt
     1621 catacgggcg acgggacctt cggcccggtc gcccggcgcg cgagtatcgc gccctcgtga
     1681 cggcggtcac gaagcggtga taacttcgaa gggcatccgg agaacatcca ccggccgacc
     1741 cgccaaaggg ggcaggcagt ccgatgagcc cgttcgcagg atccgcccac cgcacagatc
     1801 cctggcagca catccgcgtg accaggcagg acggcgtcgc caccgtcacc ctggcgcgcc
     1861 ccgagaagct caacgcgctc acctttgagg cgtacgccga cctccgcgat ctgctcgccg
     1921 agctgtccag ggaacgcgcg gtacgcgccc tggtgctcgc cggggagggg cgcggattct
     1981 gctccggcgg ggatgtcgag gagatcatcg gcgccaccct gggcatggac accggccagc
     2041 tgctggactt caaccggatg accggccagg tcgtacgggc actgcgggag tgcccgttcc
     2101 cggtgatcgc ggcggtgcac ggcgtcgcgg ccggcgccgg cgcggtactg gccctggccg
     2161 cggacttccg cgtcgccgac ccctccgccc gcttcgcctt cctcttcacc cgggtcggac
     2221 tgtccggcgg tgacatgggc gcggcctacc tgctgccccg cgtcatcggc ctcggccacg
     2281 ccacccggct gctgatgctc ggcgacaccg tccgcgcccc ggaggccgaa cgactgggac
     2341 tgatcagcga gttggccgag gagggccggg ccgacgaggc ggccgccgcc ctcgcccggc
     2401 ggctcgccga gggccccgcg ctcgcgcacg cccagaccaa ggcactgctc accgccgagc
     2461 tcgacatgcc gctcgccgcc gccgtcgaga tggacgccgc cacccaggcg ctgctgatga
     2521 acagcgccga ctacgcggag ttccacgccg cgttcaccga gaagcggacg cccaagtggc
     2581 aggggaaata gccatgcggg tcgccgtcat cggcggcggg cccggcgggc tctacgccgc
     2641 cgccctcctc aagcgcctcg accccacccg cgagatcacc gtctgggagc gcaacgcgcc
     2701 cgacgacacc ttcggcttcg gcgtggtgct ctccgacgag accctcggcg gcatcgagca
     2761 cgccgacccg gaggtctacc gcgcgctcca gcgggagttc gtccgctggg acgacatcga
     2821 catcgtgcac cggggccaca ccctgacctc cggcggacac ggcttcgcgg cactcgggcg
     2881 gcgccggctg ctgggcatcc tgcaccagcg ctgccgcgac ctcggcatcg agctgcgctt
     2941 ccggaccgag gcgccgcccg cccccgaact cgcccgcgac cacgacctgg tgatcgccgc
     3001 ggacggggtg cacagcgcca cccgcaccgc acacgccgac gccttcggcc cccggctgac
     3061 cacccaccgc tgccgctaca tctggctcgc cgccgacttc gccctcgacg ccttccgctt
     3121 cgagatcgcc gagaccgcgc acggcgtcgt ccagctgcac gcctacccct actcccgccc
     3181 gtcgcccgac cccgaccccc gccccggccc cggcgccacg acggcgcccg ccgggccgca
     3241 gcatccaggc ggcgccagca ccgtcatcgt cgagatgcgc gaggaggtgt ggcgggccgc
     3301 cgggctcgac cgcgccgacg agcgcgagtc cgcggaatgg tgcgccaagg tcttcaccga
     3361 cgccctgagc ggccgcgcgc tgcgctccaa caactccagc tggatcgctt tccgcacggt
     3421 cgtcaacgag cgctggtcgt acggcaacac ggtgctgctc ggcgatgccg cacacaccgc
     3481 gcacttctcc atcggctccg gcaccaagct cgccgtcgag gacgccctgg cgctggccgc
     3541 ctgtctccag gagcagcccg acaccgccgc cgcactgacc gcgtacgagg cggagcgccg
     3601 ccccgtcgtc gcctccaccc agcgggccgc ccgcgccagc ctcgcgtggt tcgaggagct
     3661 ggccacctac gtcgaccagc caccccgcca gttcgccttc aacctcctca cccgcagccg
     3721 ccgcgtcacc cacgacaacc tgcggctgcg cgaccccggt ttcgtcgcct cggtcgagga
     3781 cgaggcggcc gtcccggccg ggacgccgcc gatgttcacg ccgttccggc tgcgcggcct
     3841 caccctgcgc aaccgcgtgg tggtctcacc catggacatg tactccgcgc aggacggtgt
     3901 ccccggcgac ttccacctcg tccacctcgg cgcccgggcc ctgggcggcg cgggcctggt
     3961 catgaccgag atggtctgcg tcagcgaacg cggccgcatc accccgggct gcgccgggct
     4021 ctacacccct cagcaggccg cggcctggcg ccgggtcacc gacttcgtgc acgcgcaggc
     4081 gccgggcgcc gcgatcggtg tccagctcgg ccacgccggc cgcaagggct ccacgaagct
     4141 gatgtgggac ggcatcgacg aaccgctcga cgacggcaac tggcccctcg tcgccccctc
     4201 cccccttccc tacaagcccg gcagccagcg cccccaggag ctgaccgcgg gcgaactcgc
     4261 cgagatccgg gagcagttcg ccgccgccgc gcgccgcgcc gtcgactgcg gcttcgacct
     4321 gctcgaactg cactgcgcac acggctatct gctgtccgga ttcctctccc cgctgaccaa
     4381 tcaacgcacc gacgcctacg gaggttccct ctcccgccgg ctccgcttcc ccctcgaggt
     4441 cttcgacacc gtccgtgcgc tgtggccgga cgacaagccg atgacggtcc gcatctccgc
     4501 caccgactgg gccgacggcg gcaccagcgc cgaggacgcg gtggccatcg cggcggcctt
     4561 cgccgagcac ggcgccgacg ccatcgacgt ctccaccggt caggtcgtcg ccgaggagcg
     4621 ccccgacttc gggcggtcgt accagacgcc gttcgccgac cggatccgca acagcctggg
     4681 cgtcccggtc atcgcggtcg gcgccatctc ctcctgggac gacgtcaact ccctggtcct
     4741 ggccggccgg gcggacctgt gcgcgctggc ccgaccccac ctgtacgacc cgcactggac
     4801 gctgcacgcc gccgcggagc aggggtacgc ggggcagggc gccccctggc cactgcccta
     4861 tcaggcgggc agccgcaccc cgcccaccgg gcgtaccgac gcgccgaagc cccgccttac
     4921 gctgcggtga cggggaggca cgggcagcgg ggcggggcca tttcggggcg ctgagccggc
     4981 ggcggctcct cggcgcggtg tcagggggtg ggcacgctgg tgatccgcgc cgctatcacc
     5041 ggtttgccgt gctgttccac ggcgacctcg atctgccggc cgccgccggc gaccgctgtc
     5101 aggggctcgt cggcggcgcg tgcctgcacc cagcaggggg cgtccagttc ggcgtaccgc
     5161 tcgaagtcgc actccatcgc cgtcggcagg gtcggctcgg gcgtcgcggc ctgtgcggcc
     5221 tgccgggcgg cctcgatcat cagcatgccg ggggcgtggt caacgggatg gtcgaagagc
     5281 accgggtggg aggtgtccac ccgcagctgc cagcggtcgg gccggttcgt gggtgagagc
     5341 accacgtcgc gctcccggtc ccggcccacc agctgcgggg tcaccggctc gggcagcggc
     5401 agccgccggg cgttggccag cgtcaggtcg ctgtacttgc cgcgcagccg gcggtagacg
     5461 gcggggctgt gacaggagta gtcgagcgac gcggtggcca ggtccgcgcc gtcacaccgg
     5521 atgcggaagt gctgacgggc gctgatcagc cgcttgccgc ggcggatcac gtcatgatcg
     5581 gtgatgtgca gctccacctc ggccggttcg tcggccgcgt gcagggcctg cggggtgagg
     5641 tcgtagcgga cccgctgcca gatgatgggg tggtcgagcg gcacgcggtg cgcgacatgg
     5701 ctcagtaata tcccggcctg ccgcatcgtc tcgatcagta acagcggatc gtgcaggccg
     5761 cccaccgggc cgtagaagct gtgggcgcgc ggccattggg cgctcacgat ccagctgtcg
     5821 gcggcgccgc gccgccagtt cgtcaggaac acctcggaca cggcggtgcg gtggacgtac
     5881 tcccgcggca cggtcgtggt caaagcggac ggtcggtttc catcggcgaa ccggcctctg
     5941 tcgccatggc gctgatcgcg ccagaacggc atgagggtgg cgctggccat aggtatccct
     6001 ttctgaagtg cggcgctcag cggtctgagg ggctgagact cgctgccgcg gccctgcgcc
     6061 accaagatac ggaccatgtg gtttgttttc tagtgccaat gaaccgttcc tccaccccga
     6121 aagagggtga agctgtcaga cggagacctg cacggcgcgg ccgtgcggat cgagatgact
     6181 catcgcgccg ggcgaggcaa ggcccggcag ggtgtgtttc cacaacgcgg tgacccgctc
     6241 gggaagatcc gcgcggttgg tcgcggcccg cgagaacaac tgcacgccca tataggcggc
     6301 gacgaagaac tcggccgccc ggtcgggcgc cacccccggc agtaattccc cgttgtcccg
     6361 ggcctcgctg aacatcgtcg tgatgatgtc ggtccacgcc tggtacggca ccagcggctc
     6421 gtcgctgaag gtcgtctcga ccgcgatacg ggttccggcc tgcaacaacg ggtcgctgcg
     6481 cagcgccagc gccacctggt gggtgaagtc gatggcgtcc tgcagccggg actcactgac
     6541 ctgcggcagg aagggatcgg tctgctcggt gatcaccgcg cgggccagtg cgtccttcga
     6601 cgtgaagtga aagtacacgg ctcccttggt gacgccggcg cgctggatga tctcggccat
     6661 cgtggcggcc tggtagccgc gggtgccgat gacatgagcc gcggcttcga ggacggcgcg
     6721 ccgggtgcgc actgcgcgtt cctgcttgac caccgatcat cctccgtatg ctctcttgga
     6781 gttgttgcgc ccgggtgcga cccgccgtcg tggccgcgca gtctatttgc gcatcataaa
     6841 gaagccaacc atgcggtatt gaagctgcgg gtgctgcgcc gcgccgtccg tggccacgaa
     6901 tgtgaggagc tgctccccga tgacccccac cgccgttccc gacgttcccg ccatgcagcg
     6961 ggaggtccgg ctcgccgccc gcacggaagg ggagctgacc gcacggcact tcaccctcgc
     7021 cgaggtgccg gtaccggagc cgggccccgg gcaggtcctg gtgcggacgc gggtgatggc
     7081 ggtgaccgcg gcgatgcgga cccagatgac cggcgccccg ctgccgatgc cctccttcgt
     7141 gcccggccag gcgctgtggg gctccgcggt cggtgaggtc gtggcggcac ccggcggcgg
     7201 cttcgcgccc ggtgagatcg tccaccaccc ctacggctgg cgggagttcg cggtggtcga
     7261 cgcggagcgg ctgcgccggc tcgccccgga cgcgctgccc acccccgccg cccatctgtc
     7321 acagggggcg accgcctggg gtgcgctgac ccgcgcggtg gaggtccgcc ccggcgacac
     7381 ggtgttcgtc accggcgcgg cgggcggggt gggcagcctg gccgggcagc tcgcccggcg
     7441 gctgggggcc ggccgggtcg tgggcagcac cggatcggag cgcaaggccg cccggctgcg
     7501 cgccgagctg gggtacgacg acacgatcgt gcgcggggcc ggaccgatcg accggcagct
     7561 gcgccgggcg gtcccggacg gaatcgacgt actgctggac accgtgggcg gcgagcagct
     7621 caccgccgcg ctcgcgtcgg cccgccccga cgcgcggttc gcactggtcg gcgcgctcgc
     7681 cacccaactg ggcggcgacg ggggtgccgt cgcgccggtg gagctggacg cggggctgat
     7741 catcacccgg cgggtggcgc tgcggggctt cgggctgtac gcccacccgg atctgccgca
     7801 ggagtggacg aaggagttcg gtcagggcct gcgggacggc tccctggtct tcccgcacgc
     7861 cctgctgaag gggatcgagc aggcgccgcg cgcgctgtgc gaactcaccc aggggcgcca
     7921 catcggcgcc gtgctggtgg agttgtgagc ggcgtacgcc ctggggcgga cgtaccgggc
     7981 tgagggcggg gccggtcagc cggccgccgg cagccggtcc ggcgtcggga tcccggcggc
     8041 cgcgatccgc cccggtgcgg ccggcagcag ggaccagaga ccgaccacgg cctccttgtc
     8101 ccaccacgtg ccgtcctcgc cgcccaggtg cagcagaccc accacgatcg aggtcaacag
     8161 ctgggcggtg cggcgcggtt cggcgtccgg ggcgtccgcc gccggcctgc cgatcccgcc
     8221 ggtgccgcag gtcaccgcct tgtcgaggaa gagcgcgtac cagcgctgct ccagggcgta
     8281 gatgccgagg ccgggtgcgg tctccggccg cagccgcagt ccggcgcgca gcaccgcgtc
     8341 ggactccacc cgctcgaaca ggtcgacggc gaagtcctgt accgcggtgg tcagcggctg
     8401 cggggaacgg gtgaaggcgt cctcgatctc ctgcagggtg gcgagcgcgg cgtcgcgcac
     8461 cgcgagggtg aggtcgtcct tggacgtgaa gtggaagtac agggctccct tgctcaggcg
     8521 ggcccgccgg ctgatctcga ccatgcccgc ggcgggaacc cccttctcca ggaaggtctc
     8581 tgcggccgag cggatgagtg cctggcggct ctggatggcg cgatgctggg tgggcacggt
     8641 tccgtcccgt ctctagtacg gctgcgagct gattgcagtc tacatagaaa accaaccatg
     8701 cggttcgata ttcttcaggt gaatgaccgg aatcggcaca ccctgacgcg aacccgacgt
     8761 ccgcccgctc cggcggtccc tgggggagtc cgtgggacgg tgtttcctca gtctggcgcc
     8821 cggcggcggt gttgtcgacc cgtcaggatg tgacaaaggc acattgacaa acagcgcagg
     8881 cggtttttaa atctccgaag gcggtaaacg ggacgatccc ttccgttgca gccgccgcga
     8941 cggtcgcgtg ccgccccagg acagcggtgc gccatggcga gaccgcctct gccttcacct
     9001 gtcaccgacc gcaggccgcc cgaccagccg agccagcacc cgtcaccgaa ccccgtaccc
     9061 ctcatcggaa tccggaaagg tctccagcca tgcgcgtagc aacggaagtc ggcgaacgtc
     9121 ccgcgagagg tgcacggggc gccaccggtg gtgagacgat cagggccgcc gtcttcgacc
     9181 tggacgggac gcttgccaac accctcccgg cgatcagcaa actgctggtc aaggtggcct
     9241 ccgagcaggg ctgttcggtg accgcgcggc aggcggccac cgccatcggc aagccccccg
     9301 ggcccgcgtt cggccggctg ctgggccggc ccgaggacga cgaacgggtg caggccgcca
     9361 tctcccgcta ccgcgaactc ttctcctacg aagtcctgtg tcagggcccg cagctgctct
     9421 tccccggcgt gaccgcgggg ctgtccgcgc tgcgcgccca cggcgtcgaa ctggccgtcg
     9481 ccacgtccaa gaccacccgc agcgccgagg cgctgctgga cgtcatgggc atccgcgacc
     9541 tggtcggacc ggtcatcggc caggacatgg tccggcgcgg caagccgcac ccggagatgg
     9601 tgctgcgggc cgccggccgc ctgggcgtcg caccctggca gagcgcctac gtgggggaca
     9661 cggtggggga catgcggatg gcggtgaccg cgcgcatgga gccggtcgcg gtgacctacg
     9721 gcgtcggcac gttcggcgag ctggccgcgg tcgccgggac ccggatgtgc agctcgttca
     9781 aggacgtggt gtcggtgatc gccgccgccc gaccgcgggc ggcggccggt gcgccgcacc
     9841 gtccggccgt cgcggtgggg ggcccggcgc gacgacgctg agggcggcgt cctcacggcc
     9901 cggccgtcac ctcgcgcacg tagcgggccc ccgcgtcctg gagcgtgccg tgcaaccggc
     9961 cgaacacctc cgcggcgcgg ccgccgggcc agccctccgg caggagggga gagggcagtc
    10021 cgggatccgc gtacgggaga cggcgccagg cgtccagcgc cagcagatag tcgtggtagg
    10081 ccgcttccgg cggcaccgtg cgccgccggg accagcggcg cagcaccggc tcctgctccg
    10141 ccaggaacgc gcggtgctgc gcggcgacgg cgtccaggtc ccaccagcgc gccaccgcgt
    10201 ccgccgtcgg cgcgaaaccg gcgtgcgtac cggtgaagag gtcgacgtac gggtccagtt
    10261 ggagccgttc gagggtgtgc cgggtctcct cgtagagctg ggcgggggcg atccacacac
    10321 ccggtgccgc ggtgccgaag ccgaggcggg ccaggcgcga gcgcagcagg tgacgcttgt
    10381 gccgctcctc ctcggggacg gagaagacgg ccagcaccca gccgtcggac agccgcgcgg
    10441 ccggccggcc gaagatccgc cggtcgccgt cctccagcag ctgacgggcg gcgtccgaca
    10501 gcccgtacgc ggccgccccg gcctcggtgc gcccggggac gagcaggccg cgccgcttga
    10561 gccgggagac cgcggagcgc accgacggcg gatccacgcc cagcacaccg agcagccgga
    10621 tcagcgcggc gaccggcagc ggggcggccg cgggccccgt ggcgccgggc tcgccgcgtc
    10681 cgtaggcgcc gtagaacgtg acgatcaggg accgtggggt gcgctgcgtt tgctggtcgg
    10741 tcaccgcttc actccagatc gggtgcgcgc agccggaagc gctggagctt gccggtgggg
    10801 gtgcggggca gggcggtgtg gaagacgatg tcgcgcgggc atttgtaggg cgcgatacgg
    10861 gtcttggtga aggcgcgcag cgcggcgacg gtgtcgtccc cgc
//
