LOCUS       NZ_CP019779            10213 bp    DNA     linear   CON 14-MAR-2024
DEFINITION  Streptomyces sp. MOE7 chromosome, complete genome.
ACCESSION   NZ_CP019779
VERSION     NZ_CP019779.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MOE7
  ORGANISM  Streptomyces sp. MOE7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8399509)
  AUTHORS   Elnahas,M.O., De Leon,K.B., Amin,M.A., Hussein,M.M.D., Ali,A.E. and
            Wall,J.D.
  TITLE     Complete Genome Sequencing of Streptomyces sp. Strain MOE7, Which
            Produces an Extracellular Polysaccharide with Antioxidant and
            Antitumor Activities
  JOURNAL   Genome Announc 5 (22), e00442-17 (2017)
   PUBMED   28572320
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399509)
  AUTHORS   Elnahas,M.O., De Leon,K.B., Amin,M.A., Hussein,M.M.D., Ali,A.E. and
            Wall,J.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2017) Biochemistry, University of Missouri
            Columbia, 117 Schweitzer Hall, Columbia, MO 65211, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 8.1
            Genome Coverage                   :: 180.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002090335.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 02:19:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,435
            CDSs (total)                      :: 7,344
            Genes (coding)                    :: 7,005
            CDSs (with protein)               :: 7,005
            Genes (RNA)                       :: 91
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 339
            CDSs (without protein)            :: 339
            Pseudo Genes (ambiguous residues) :: 0 of 339
            Pseudo Genes (frameshifted)       :: 230 of 339
            Pseudo Genes (incomplete)         :: 155 of 339
            Pseudo Genes (internal stop)      :: 7 of 339
            Pseudo Genes (multiple problems)  :: 47 of 339
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:50:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1152839
            Orig. end                         :: 1163052
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP019779.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Judy D. Wall 117 Schweitzer
            Hall Columbia, MO USA 65202.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10213
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10213
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10213
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5213](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5213)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    303..312
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            complement(1708..3996)
                     /locus_tag="STRMOE7_RS05300"
                     /old_locus_tag="STRMOE7_05290"
     CDS             complement(1708..3996)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606566.1"
                     /locus_tag="STRMOE7_RS05300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_05290"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_084769882.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.2e-37, bitscore:
                     127.4, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPNDSAAPQAQLPPPGRGRHRPEPAPGARRTRRAAPPAPKPKK
                     ARTVRTPTVLQMEAVECGAASLAMVLAHFGRHVPLEELRIACGVSRDGSRASNLLKAAR
                     SYGLQAKGMQMEPAALAEVQAPAILFWEFNHYVVYDGTGRRFGRRGVYINDPDKGRRFV
                     PTEDFDTSFTGVALVFEPSESFRKGGRKPGVLSAVPARMRGTTGTLLAALLASLLLVAV
                     GAAVPALSRTYIDMFLIGNQTSLLGPLFASMAAMVALTAVLTGLQQANLLRGRIISSTL
                     TSARFLRHLLRLPVTFFAQRSPADLVQRLQSNDAVAETLARDLAAAGVDGIVVVLYAFL
                     LWSYDPQLTVIGVGIALLNVVAMRIVVRLRATHTAKLRADSARLTNTAYTGLQLIETMK
                     ATGGENGYFRRWAGQHATTLEVQQRLGVPSAWLAVVAPTLATLNSALILWIGGMRAVEG
                     HISIGLLVAFQALVTRFTAPLTRLNGVAGRIQDFAADVARLKDVESFPADSLYARPGSD
                     ADTRRLKGHVTLENLTFGYSPLDKPLLSGFSLTVGPGRQVALVGGSGSGKSTVSRLISG
                     LYSPWEGTIRIDGRRLEDLSRSALAASVSFVDQDVFLFEGTVRDNVALWDPSIPDDAVI
                     TALKDAALYDDVIARRPDGIYSRVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTS
                     ALDARTEQIIMDNLRRRGCACVVIAHRLSTVRDSDEIVVLDHGVVVERGRHEDLVAAGG
                     AYAELVKEH"
     gene            complement(3993..4799)
                     /locus_tag="STRMOE7_RS05305"
                     /old_locus_tag="STRMOE7_05295"
     CDS             complement(3993..4799)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="STRMOE7_RS05305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_05295"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_084769883.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSPEEMDLPVRFARPQGWLVLIVTVAVLIGASFW
                     AVTGTVSSTLNAPGILTHGQGSYVLQSPVAGQVTAVLAEEGQRVAANTPLLRVRTDRGT
                     TVVRSLAAGRLTTMVATIGSVVTTGADVASVERVAHRGDPLTATLYVPADNGAGIPVGG
                     SVDLTVQSVPSQQYGVLRGRVKAVGRTAQTRQRIGAYLGSSQLGEQFSKSGPPVAVLVQ
                     LDPAPHNKSGYAWSTSGGPPYAVDSMTLASGAIHLAAQHPIDWLLP"
     misc_feature    4771..4788
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.48"
                     /tool="antismash"
     gene            complement(5001..5213)
                     /locus_tag="STRMOE7_RS05310"
                     /old_locus_tag="STRMOE7_05300"
     CDS             complement(5001..5213)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018849411.1"
                     /locus_tag="STRMOE7_RS05310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_05300"
                     /product="hypothetical protein"
                     /protein_id="WP_033270189.1"
                     /sec_met_domain="strepbact (E-value: 1.1e-23, bitscore:
                     82.2, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREIQTQEIADSELDNVAGGLVGNLAGTATSTVDGATGLNVGGTL
                     GTVTGTVEGLTGVNTGQVTGLVAGL"
     misc_feature    complement(5231..5245)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.24"
                     /tool="antismash"
     misc_feature    complement(5423..5436)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.87"
                     /tool="antismash"
     misc_feature    5600..5609
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.44"
                     /tool="antismash"
     misc_feature    complement(5775..5790)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.12"
                     /tool="antismash"
     misc_feature    5778..5793
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.27"
                     /tool="antismash"
     gene            5975..7006
                     /locus_tag="STRMOE7_RS05315"
                     /old_locus_tag="STRMOE7_05305"
     CDS             5975..7006
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091739.1"
                     /locus_tag="STRMOE7_RS05315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_05305"
                     /product="PHP domain-containing protein"
                     /protein_id="WP_084769885.1"
                     /transl_table=11
                     /translation="MEPVEALERIAFLLERQGAETYRVQAFRTAAAAIRELPDDELRRR
                     AEDGTLTRLKGLGPKTTRVVEEAFAGVRPVYLARLEDEAGGPLVDGEQGQRLRRALRGD
                     CHTHSDWSDGGSPVEEMARTARDLGHAWCVLTDHSPRLTVARGLSAERLREQLALVAEV
                     NEVLAPFRLLTGIECDILDDGTLDQEPELLDRLDVVVASVHSKLRMDKGPMTRRMLAAV
                     RNPLVDVLGHCTGRLLTRKPRPESQFDADEVFAACAEHRTAVEINCRPERLDPPRRLLR
                     RAVDAGCLFSIDTDAHAPGQLDWQLYGCARAEECGVPAERVINTWTERQLATWTRTGRT
                     PHG"
     gene            complement(7019..8377)
                     /locus_tag="STRMOE7_RS05320"
                     /old_locus_tag="STRMOE7_05310"
     CDS             complement(7019..8377)
                     /GO_function="GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /GO_process="GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606562.1"
                     /locus_tag="STRMOE7_RS05320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_05310"
                     /product="chloride channel protein"
                     /protein_id="WP_084769886.1"
                     /transl_table=11
                     /translation="MAVGPTGPGGGTPPGVPQDPYAPARTRGYLKLLLIAAVIGVPVCA
                     VSFGFLALVHELEPLIMQALPRALGFAGTPWWWPVPMLVVAGLLVGLTVRHLPGHGGHE
                     PADGLALSGAPALSALPGIVLAALASLSFGAVLGPEAPLIALGGGLSAGAARLVGRGLP
                     ERAVAALSAAGSFAAVSALLGSPLLGAFLLMEVAAPVGPLLGALLLPGLLASGVGALIF
                     TGLGDWTGLGTYSLALHDIPRASGPTLSEFGWALVIGLAAACFGGGVRRLSLLLKERVG
                     RHRVPMTVAMGLVVGALAAGFALATGKPATEMLYSGQSALGSLFRDSAGYSVGALLLLV
                     VCKGLAYCASLSAFRGGPIFPALFLGAAGGVILSHLPGLSLTAGFAMGVGALGVAMLRL
                     PMTSVLLATLLMGSNGLTVMPLVIVAVVVAHVAALRLTPATAPATPAPAPSAG"
     gene            complement(8472..9380)
                     /locus_tag="STRMOE7_RS05325"
                     /old_locus_tag="STRMOE7_05315"
     CDS             complement(8472..9380)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091736.1"
                     /locus_tag="STRMOE7_RS05325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRMOE7_05315"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_033270192.1"
                     /transl_table=11
                     /translation="MIEARRLHILRAVADHRTVTAAAAALYLTPSAVSQQLAALEQETG
                     HRLVDRSARGVRLTAAGDILLTHANAVLAQLERAESELAAYGSGEAGTVTVAAFATGIG
                     LVVAPALTALARSAPGIRVRVQDAEGDASLRMVLDRQVDVAVAVEYRGAPRADDPRLTR
                     VPLYAEPFDAVLPTGHRLAATPQVAVAALADDLWIGPYPGNPCHDVVVLACENAGFQPR
                     LEHSSDDFRAVVALASAAAGVALVPRSALRDVDLTGVVVRPVDGVAPTRRVFAAVRRGA
                     EDHPLFRPVLAALREAAAPQG"
     misc_feature    complement(9345..9361)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.67"
                     /tool="antismash"
ORIGIN
        1 aggaagaccg cgccgatgac gagcagcatg gcgagcgcgg cgagcgccag cggcacgctg
       61 tagacgagca gcaggacgag gttcatcgcg ccgatcgtcg tggactgcac ggccaccggc
      121 ccgatgccgg acagcacccg ccggatggca ctgatgccca tcgcggcact ggccagctcg
      181 ccggtggagc gctcggtgaa gaacttcgtc ggcagccgca gcagccggtc ccagacggcc
      241 ggctggagcg cgctctcgat acggccctcc atccgcagca cggtgaggtt ctgcagcagc
      301 atgaaggcgg ccgagacgat gctggtgacg atgacggcca gggacacctg gacgatcagg
      361 ctccgctcgg cggccggtac gtacacgccg agcaccttgc cggtcgcgat cgggacgagc
      421 gcgcccaggc cgaccgccac caggccgctg agcgccaggt tgcggacgtc cttccggctg
      481 ccgcgcagac tgaagcgcat cagccgccac atgctcatcg gccgctccgg cagcgggcgg
      541 tagaacatca ccgcgcgcga ctcgaactcg tcggcgttgt ccttgccgac gcggatgcgc
      601 aggccggacg cgggattcac ggcctcgtag cggccgcggc gccacagcag cgcgacgggg
      661 gcaccggact tggcgcgatg gccgaccagc gggcctgcgt ccgtccgcca ccaggagccg
      721 gtgagccgga ccgcgcgggt gcggatccgt gagctgaccg cgatccgctc cacggggctg
      781 acccggtcgt tgacggcgcc gccccgcggc ggtgcggtga gcgtgatccc ggccgcctcg
      841 gccaccgccc ggcagacctc gaaggtggtg tcgtcaccgg cccggttggt gacgtcgccg
      901 ccccgcccgg agcggtcctg gcggccgatg gacgccagca gggcctggtc ggcgcgctcg
      961 cggaccgcct cgccggcctt gatgccggcc gccgcgcggt cctcgtgggc acgctccagc
     1021 tgctcgatcc agcggtcgac ggcggacagc agccggtact gctggttgac catccgctgc
     1081 cacaggtcgg ggtccaccag cagatcggcg gccgcctccg cgctgtagga ggcgccgtac
     1141 tgcacactgc cgggcggtac gggcatccac agcacgtcgt cgtcggcgac cgcctcgtcg
     1201 cccggccggc cgtccagcgg cgcctcgaac agcaccccca ggctgcgggc ggtgcccagg
     1261 gcgacggcgt gctccagtgc ggagggctgc tcggcctggg tggcgtactg cgggtaggac
     1321 ccggtgtccc cgtactcccc gtaggcgccg tagtcgtggc ggggcagttc gcgcagcggg
     1381 atccggcgca gcccgcagtc ctgcgagggg cggccgagca gggtgtgctg ggggccgtcg
     1441 accgggccga gcagcagggt gccggcttcg agccggccga ggaagtgcca gtgtccctcg
     1501 gcggcggcgt cgaccgcgaa gaggtcgagc gcgcccccgg tgacgagcca gagcacgtgc
     1561 ggcccctcca gggggacgct gcgcagaccg gcgcagtgga cgggcgcgcc gaggccgccc
     1621 agggcctgcg tcaccgcgtc cgtcccgccg ggccccgcgg cggggaccgg cgacggggac
     1681 gagggggacg acgacatggc tgccacgtca gtgctccttg accagctcgg cgtacgcgcc
     1741 accggcggcg accaggtcct cgtgccggcc tcgttcgacg accacgccgt ggtcgaggac
     1801 cacgatctcg tcgctgtcgc ggacggtgct cagccggtgg gcgatgacga cgcaggcgca
     1861 gccgcgccgc cgcaggttgt ccatgatgat ctgttcggtc cgggcgtcca gggcgctggt
     1921 gacctcgtcg aggaccagga tgctggggcg ccggaccagg gcgcgggcga tctccagccg
     1981 ctgccgttgt ccgccggaga agttgcggcc gtcctgctcg acgcggctgt aaatgccgtc
     2041 gggacgccgg gctatgacgt cgtcgtagag cgcggcgtcc ttcagggcgg tgatgaccgc
     2101 gtcgtccggg atcgaggggt cccacagcgc cacgttgtcg cggacggtgc cctcgaagag
     2161 gaagacgtcc tggtcgacga aggacaccga cgcggcgagc gcgctgcggg agaggtcctc
     2221 caggcgccgg ccgtcgatgc ggatggtgcc ttcccagggg ctgtagaggc cggagatcag
     2281 ccgggagacg gtggacttgc cgctgccgga gccgccgacg agggccacct gccggccggg
     2341 gccgacggtc agcgagaagc cgctgagcag cggtttgtcc agcgggctgt agccgaaggt
     2401 gaggttttcc agcgtcacgt gacccttgag ccggcgggtg tcggcgtccg agccggggcg
     2461 ggcgtagagg ctgtcggcgg ggaaggactc gacgtccttg aggcgggcca cgtcggcggc
     2521 gaagtcctgg atccggccgg cgacgccgtt gagccgggtc agcggcgcgg tgaagcgggt
     2581 caccagcgcc tggaaggcga ccagcaggcc gatggatatg tgcccctcga ccgcccgcat
     2641 gccgccgatc cacaggatca gcgcgctgtt gagggtcgcg agggtggggg cgaccacggc
     2701 cagccaggcg ctcggtacgc cgagccgctg ctggacctcc agcgtggtgg cgtgctggcc
     2761 ggcccagcgg cggaagtagc cgttctcccc gccggtggcc ttcatcgtct cgatgagctg
     2821 gaggccggtg taagcggtgt tggtcagccg ggcgctgtcg gcccgcagct tcgccgtgtg
     2881 ggtggcgcgc aaccgcacca cgatgcgcat cgcgacgacg ttgagcagtg cgatgcccac
     2941 gccgatgacg gtcagttggg ggtcgtagct ccacagcagg aaggcgtaga ggacgacgac
     3001 gatgccgtcg acgcccgccg cggccaggtc gcgggccagg gtttcggcga ccgcgtcgtt
     3061 ggactgcagc cgctggacga ggtcggccgg gctgcgctgg gcgaagaagg tgaccgggag
     3121 tctgagcagg tggcgcagga agcgggcgct ggtcagggtg gaggagatga tccggccgcg
     3181 cagcaggttg gcctgttgca gcccggtcag tacggcggtc agcgcgacca tcgcggccat
     3241 cgaggcgaag agcggcccca gcagcgacgt ctggttgccg atcaggaaca tgtcgatgta
     3301 cgtacggctc agcgcgggca ccgcggcgcc gaccgcgacc agcagcaggc tggcgagcag
     3361 ggcggcgagc agggtgccgg tggtgccgcg catccgggcg ggcaccgcgc tcagcacgcc
     3421 gggcttgcgg ccgcccttgc ggaagctctc ggacggctcg aagaccagcg cgacaccggt
     3481 gaagctggtg tcgaagtcct ccgtcgggac gaagcggcgg cccttgtcgg ggtcgttgat
     3541 gtacacgccg cggcggccga agcggcggcc ggtgccgtcg tagacgacgt agtggttgaa
     3601 ctcccagaac aggatggcgg gggcctgcac ctcggcgagc gcggccggtt ccatctgcat
     3661 gcccttggcc tggagcccgt aactgcgggc ggccttgagc aggttgctgg cgcgcgagcc
     3721 gtcgcgggag acgccgcagg cgatccgcag ctcctccagc ggcacatggc gcccgaagtg
     3781 ggcgaggacc atggccaggg aggcggcgcc gcactccact gcctccatct ggaggaccgt
     3841 gggggtgcgg acggtcctgg ccttcttggg cttgggggcg ggcggcgcgg cacggcgggt
     3901 gcggcgggcg ccgggcgcgg gctcggggcg gtggcggccg cggccgggcg gcggcagctg
     3961 cgcctgcggg gccgcggagt cgttcggtgc ggtcacggca gcagccaatc gatcgggtgc
     4021 tgcgcggcca ggtggatggc gccgctggcc agcgtcatgg agtcgacggc gtagggcggg
     4081 ccgccggagg tcgaccaggc gtagccggac ttgttgtgcg gcgccgggtc gagttgcacc
     4141 aggaccgcga cgggcggccc ggacttcgag aactgctcac cgagctggct gctgccgagg
     4201 taggcgccga tccgctggcg ggtctgggcg gtgcggccga cggccttcac ccggccgcgc
     4261 agcacgccgt actgctggct gggcacggac tgcacggtga ggtcgaccga gccgcccacg
     4321 gggatgccgg cgccgttgtc ggcggggacg tacagggtcg cggtcagcgg gtcgccgcgg
     4381 tgcgcgacgc gctcgacgga ggcgacgtcc gcgccggtcg tgacgacgga tccgatggtg
     4441 gcgaccatgg tggtcagccg gccggcggcg aggctgcgca cgacggtggt gccccggtcg
     4501 gtccggaccc tgagcagcgg ggtgttggcg gccacccgct ggccctcttc ggcgaggacg
     4561 gcggtgacct ggcccgcgac ggggctctgc aggacgtagc tgccctggcc gtgggtgagg
     4621 atgccggggg cgttgagcgt ggaggagacg gtgccggtca ccgcccagaa ggacgcgccg
     4681 atcagcacgg cgaccgtgac gatcagcacg agccagccct gggggcgggc gaagcgcacc
     4741 ggaagatcca tttcctccgg cgactgcagc ttggaaaggg cctgttgacg gaactgcacg
     4801 acactcttcc ctcaactgca acgatctgtc aggacaccgc ggacggggcg gctcgccacg
     4861 gttgccgcgg tggagccgag cctacggcgg cccgtccggg gctcggcagc cgggtgcccc
     4921 ggaacccggc gaaggccagg cggttccggg gcagccggcc ggcatgtcgg caaggagggg
     4981 atgccgacct gccgtgggtc tcagagaccg gcgacgaggc ccgtgacctg gccggtgttg
     5041 acgccggtca gaccctcgac ggtgccggtg acggtgccca gggtgccgcc gacgttgagg
     5101 ccggtggcgc cgtcaacggt ggaggtggcg gtgccggcca ggttgcccac gaggccgccg
     5161 gccacgttgt cgagctcgct gtcagcgatt tcctgggtct gaatctcgcg catggtgatt
     5221 cctttcggat acatatctca aaatagctgc gaccgagtcg cccggcaggg cggtccggcc
     5281 gcggcagcgg tcgccgggac cctcaagact gagaacccag tgcgacttcc gcggtgtgaa
     5341 ggatcaaagc atgcaggccc gttgccctgc taatcggaca accgccgatc cgggtgtcat
     5401 cgcaccattg aattacgtgt cgtgcacgca catccatgaa tcggtggcag cttcttcaca
     5461 actccttcgg tttcacttcc accagagcgt cccggccgcg ttcggcgaac tggacattcc
     5521 cgcaccaaac cgatgaggcg caggtcagca gggggaaccg cctccgacgg ccgtgccctg
     5581 actgcgcatt cgatgtggag gttttttgaa ggccgtcaat ttttacggca ccgattcacc
     5641 cggtggtcgg aagatcggcg cacctgccac ccgacgccct tcatcgaatg ttgcgggagc
     5701 tttgcgcgag ttttaaccgc gcccgattca tccgaagttc ccggggcggt attgcacgcc
     5761 ttgcacacct tgcagggaat gatcagcact aaccatgcgt ctttccgaag caatcggatc
     5821 gaccggcccg agaacggaca taaccgcata acggcacggc ttccgactcg gcggaaatgg
     5881 gcgatttacc gccggtacca cccccggtgg cagccgtccc cggctggggc accgtgaaag
     5941 ggacctgggc cggcgcaccg ggaaggagcc gcacgtggag cccgtcgaag cgctggagcg
     6001 gatcgccttc ctgctggagc ggcagggcgc ggagacctat cgcgtacagg cgttccgtac
     6061 ggccgccgcg gcgatccggg agctgcccga cgacgagctg cgcaggcgcg ccgaggacgg
     6121 caccctcacc cgcctcaagg ggctcggccc caaaaccacc cgggtggtcg aagaggcgtt
     6181 cgcgggagtg cgcccggtgt atctggcgcg cctggaggac gaggcgggcg ggccgctggt
     6241 cgacggagag caggggcagc ggctgcggcg ggcgctgcgc ggggactgcc acacgcactc
     6301 ggactggtcg gacggcggca gcccggtcga ggagatggcc aggaccgccc gcgacctcgg
     6361 gcacgcctgg tgcgtgctga ccgaccactc cccccgcctc accgtcgccc gcgggctgtc
     6421 cgccgaacgg ctgcgggagc agctggcgct ggtcgccgag gtgaacgaag tgctggcgcc
     6481 gttccggctg ttgaccggga tcgagtgcga catcctcgac gacggcacgc tcgaccagga
     6541 gccggagctg ctggaccggc tcgacgtggt ggtggcctcg gtgcactcca aactgcggat
     6601 ggacaagggc ccgatgacgc ggcggatgct cgcggcggtc cgcaacccgc tggtggacgt
     6661 gctcgggcac tgcaccggac ggctgctcac ccgcaaaccg cgccccgagt cgcagttcga
     6721 cgccgacgag gtgttcgcgg cctgcgcgga gcaccgcacc gcggtggaga tcaactgccg
     6781 tccggagcgg ctggaccccc cgcgccggct gctgcgccgt gccgtggacg cgggctgcct
     6841 gttctccatc gacaccgacg cccatgcgcc gggccagctg gactggcagc tctacggctg
     6901 cgcccgggcc gaggagtgcg gcgtccctgc ggagcgggtc atcaacacct ggacggagcg
     6961 ccagctggcc acctggaccc gtacgggccg caccccgcac ggctgagccc ggaccgggtc
     7021 agccggcgga cggcgcgggc gcgggcgtgg ccggtgccgt cgccggggtg agacgcagcg
     7081 cggcgacgtg cgccacgacc acggcgacga tgaccagcgg catgacggtc agtccgttgc
     7141 tgcccatcag cagcgtggcc agcagcaccg aggtcatcgg cagtctgagc atcgccaccc
     7201 ccagcgcgcc gacgcccatc gcgaagcctg cggtgagcga cagtccgggc aggtgggaca
     7261 ggatgacccc gccggccgcg ccgaggaaca gcgccgggaa gatcgggccg ccccggaagg
     7321 cgctgagcga cgcacagtag gccaggccct tgcacaccac gaggagcagc agcgccccca
     7381 ccgagtagcc ggcgctgtcc cggaacagcg acccgagcgc cgactgcccc gagtagagca
     7441 tctcggtcgc gggcttgccg gtggccaggg cgaacccggc cgccagcgcc ccgaccacca
     7501 ggcccatcgc caccgtcatc ggcacccggt gccgcccgac ccgctccttc agcagcagcg
     7561 agagccgccg tacgccgccc ccgaagcagg ccgcggccag cccgatgacc agcgcccagc
     7621 cgaactccga gagcgtcggt ccggaggccc tggggatgtc gtgcagcgcg agcgagtacg
     7681 taccgagtcc ggtccagtcc cccagcccgg tgaagatcag cgcgccgacg ccggacgcga
     7741 gcaggccggg cagcagcagc gcgcccagca gcggaccgac cggcgccgcc acctccatca
     7801 gcaggaacgc gcccagcagc ggggagccga gcagcgcgct gaccgccgcg aagctgccgg
     7861 cggcactcag cgcggcgacg gcccgctccg gcaggccccg gcccaccagc cgtgcggcgc
     7921 ccgcggacag cccgccgccg agcgcgatca gcggcgcctc cggcccgagc accgcaccga
     7981 agctgaggga ggccagagcc gcgaggacga tgccggggag cgcggagagc gcgggcgcgc
     8041 cgctgagcgc cagcccgtcg gccggctcat ggccgccgtg gcccggcagg tggcggaccg
     8101 tcagcccgac cagcaggccg gccaccacca gcatgggcac cggccaccac cacggcgtcc
     8161 cggcgaagcc cagcgcccgc ggcagcgctt gcatgatcag cggttccagt tcgtgcacca
     8221 gcgccagaaa gccgaacgac accgcgcaga ccgggacgcc gatcaccgcc gcgatcagca
     8281 gcagtttcag atagccgcgg gtgcgggccg gcgcgtaggg gtcctggggc acaccgggag
     8341 gtgtcccgcc gcccgggccg gtggggccga ccgccatcgc ctcgcctcct cgccgccgcc
     8401 acgaggagcc tgccacggcc ggacccggcc ggtgcgcagg acgcggtccg cgccacccga
     8461 acgccgtcgc gtcacccctg cggggccgcc gcctcccgca gcgcggccag caccgggcgg
     8521 aacagcggat ggtcctcggc tccccggcgt accgcggcga acacccggcg ggtgggcgcg
     8581 actccgtcga cgggccggac caccaccccc gtgaggtcca cgtcgcgcag cgccgagcgc
     8641 ggcacgagcg ccacgcccgc cgcggccgag gcgagggcga cgaccgcgcg gaagtcgtcg
     8701 gaggagtgct ccagccgggg ctggaagccg gcgttctcgc aggccaggac gaccacgtca
     8761 tggcaggggt tgccggggta ggggccgatc cagaggtcgt cggcgagcgc ggcgaccgcc
     8821 acctggggcg tcgcggcgag ccggtgcccg gtgggcagga ccgcgtcgaa cggctccgcg
     8881 tagagcggga cgcgggtcag ccgggggtcg tccgcgcggg gcgcgccgcg gtattcgacg
     8941 gccaccgcca catcgacctg ccggtcgagg accatccgca ggctggcgtc gccctcggcg
     9001 tcctggacgc ggacccggat gccgggcgcg gaacgggcga gcgcggtgag cgcgggcgcg
     9061 acgaccagtc cgatgccggt ggcgaaggcc gcgacggtga ccgtgccggc ctcgcccgag
     9121 ccgtacgcgg cgagctcgga ctcggcgcgc tccagctggg cgaggacggc gttggcgtgg
     9181 gtgagcagga tgtcgcccgc ggcggtcagc cgcacaccgc gggcgctgcg gtcgacgagg
     9241 cggtgcccgg tctcctgctc cagcgccgcc agctgctggg agaccgctga cggcgtgagg
     9301 tagagcgcgg cggccgcggc ggtgaccgtg cggtggtcgg ccaccgcacg gaggatgtgc
     9361 agccggcgtg cttcgatcat gtggctattt tcgcaggccg gccgcactgg tggcgtcact
     9421 ccgcggcggc ggcccccgcc tcggccgcca tggcggtccg cgcggccacg aaggcgtcca
     9481 ccgcgcggtt gacgtcctcg gtggagtgcg cggccgacag ctggacgcgg atccgcgcct
     9541 ggccctgcgg gacgaccgga tacgagaagc cgatgacgta caccccgcgc tccaggagca
     9601 gctccgccat gcgggccgcc tcggccgcgt cgccgatcat gacgggggcg atggcgtggt
     9661 cgccggggag gacgtcgaag ccctccgcgg tcatccggga gcggaacagc gcggtgttgg
     9721 cctgcagccg ctcgcgcagc tcgccggccg actccagcag gtcgaggacc ttcagcgagg
     9781 cggcggcgat caccggggcg agcgagttgg agaagaggta cgggcgcgag cgctggcgca
     9841 gcagggcgac gatctcggcg cgggccgcga cgtagccgcc ggaggcgccg ccgagggcct
     9901 tgccgagggt gccggtgatg atgtcgacgc ggtccatgac gccgtgcagc tcgggggtgc
     9961 cgcggccgcc ggggccgacg aagccgaccg cgtgcgagtc gtcgaccatc accatcgcgc
    10021 cgtggcggtc ggccagatcg cagatctcgt ccaggggtgc cacgtagccg tccatcgaga
    10081 agacgccgtc ggtgacgatc agggtccggc cggcgcctgc ttcggcggcc tccttcaact
    10141 ggcgttccag gtcggccatg tcgcggttgg cgtagcggta gcggcgggcc ttggacagcc
    10201 ggatgccgtc gat
//
