LOCUS       NZ_CP020557            10894 bp    DNA     circular CON 24-JUL-2024
DEFINITION  Paenibacillus larvae subsp. pulvifaciens strain SAG 10367
            chromosome, complete genome.
ACCESSION   NZ_CP020557
VERSION     NZ_CP020557.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus larvae subsp. pulvifaciens
  ORGANISM  Paenibacillus larvae subsp. pulvifaciens
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 4666454)
  AUTHORS   Dingman,D.W.
  TITLE     Paenibacillus larvae genome sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4666454)
  AUTHORS   Dingman,D.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2017) Entomology, CT Agric Expt Station, 123
            Huntington St, New Haven, CT 06504, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2016
            Assembly Method :: SMRT Pipe HGAP assembly v. February-2016
            Assembly Name                     :: sag10367-hgap-022916
            Genome Coverage                   :: 214.2x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002082155.1-RS_2024_07_24
            Annotation Date                   :: 07/24/2024 02:27:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,275
            CDSs (total)                      :: 5,166
            Genes (coding)                    :: 4,634
            CDSs (with protein)               :: 4,634
            Genes (RNA)                       :: 109
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 532
            CDSs (without protein)            :: 532
            Pseudo Genes (ambiguous residues) :: 0 of 532
            Pseudo Genes (frameshifted)       :: 400 of 532
            Pseudo Genes (incomplete)         :: 184 of 532
            Pseudo Genes (internal stop)      :: 26 of 532
            Pseudo Genes (multiple problems)  :: 72 of 532
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:07:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4232971
            Orig. end                         :: 4243865
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020557.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Dr. Adriana Alippi,
            CIDEFI-CIC, Facultad de Ciencias Agrarias y Forestales, Universidad
            Nacional de La Plata, 1900 La Plata, Argentina.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10894
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="19"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     cand_cluster    1..10894
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10894
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5894](+)"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5894
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     gene            <60..299
                     /locus_tag="B7C51_RS28080"
                     /pseudo=""
     CDS             <60..299
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013351078.1"
                     /locus_tag="B7C51_RS28080"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="HTH domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="SNLPKGLNPATMRQIIYFMRQQKKALSAEEVADGVGIARVTARRY
                     LDYLEKIRQVRLVISYGGIGRPINRYEFYLSNRK"
     misc_feature    complement(420..429)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            899..1381
                     /locus_tag="B7C51_RS22755"
                     /old_locus_tag="B7C51_22750"
     CDS             899..1381
                     /EC_number="3.1.3.48"
                     /GO_function="GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886755.1"
                     /locus_tag="B7C51_RS22755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_22750"
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="WP_077997503.1"
                     /transl_table=11
                     /translation="MVRVLFVCLGNICRSPMAEAVFKHLVKEAGLEKEIEVDSAGTGDW
                     HIGHAPHEGTRKLLDRQGISYEGMKARQVAKSDSEQFDYIIMMDAKNVKDVRRIFGDSG
                     HPKMFRMMDFALDETVRDVPDPYFTGNFEEVYEMLLKSCKHLLQQVIEEYHLSPKQ"
     misc_feature    complement(1431..1444)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 24.02"
                     /tool="antismash"
     gene            1545..2699
                     /locus_tag="B7C51_RS22760"
                     /old_locus_tag="B7C51_22755"
     CDS             1545..2699
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /locus_tag="B7C51_RS22760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_22755"
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="WP_237088992.1"
                     /sec_met_domain="ATP-grasp_3 (E-value: 7e-12, bitscore:
                     43.9, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 5.7e-08,
                     bitscore: 30.7, seeds: 12, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQGHILFFNATYIKKELILRTAKDLGLTVSVVGPKLPKWSKRYVD
                     YFIETNTYNIEQTLSVLRKYHKKRPFNGVITFWDRDVEPVAHVANEFNLPASSKTAAAR
                     ARNKSLMRKALKLRNVPHPKFIHISGSWTELQQAAEKIGYPLIYKPVGASASKGVFKVE
                     SADQLRRTWEKMMKHATPENDKMFTFYCDEYLVEEFISGKEVSVEGVVAKGKIHIVGIT
                     EKWADSINFTEYQHAFPARLPEAVVEDIYKVTRKGIDALELDNCGFHAELIITDHGSKI
                     IEVAGRLGGDFITTHLIPLAYGSNITRANILTVLGEDFDLRPTKKRGACIRFLLAEKGG
                     TVRQWMGTENVMNIPGVVHFGFDKQVGDTVVMPLKNLWTSVFVT"
     gene            2776..3081
                     /locus_tag="B7C51_RS28085"
     CDS             2776..3081
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B7C51_RS28085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_237088993.1"
                     /transl_table=11
                     /translation="MSRKRLFFIGIPETHVKRTLSRCKKLGYEIILGDTKNNLKLHADR
                     LVITDFRDEKNLRDVTCSLQEESPLDAIFTFKEFGLINTSKLLHEYGLRGKSNRSN"
     gene            3134..3964
                     /locus_tag="B7C51_RS22765"
                     /old_locus_tag="B7C51_22760"
     CDS             3134..3964
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /locus_tag="B7C51_RS22765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_22760"
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="WP_237088994.1"
                     /sec_met_domain="ATP-grasp (E-value: 1.5e-06, bitscore:
                     26.1, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 3.4e-07,
                     bitscore: 28.1, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 1.3e-08, bitscore:
                     33.2, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPDYELCESLEDAKKLLKRIEGPIIIKPHNLQGSIGVYKVEQEE
                     DLQVLYEKCLTHCNEPIVMAEEFIVGQEVSIEAIVYRGKTIIFGVTEKLLYPGTFVEAG
                     HISPYAGTEMSREEYTKLVERIVGAMKIQMGPLHIEGFHTEKGFYVGEVHTRYGGDNIT
                     TLTELALKCDMTSPIFAELGDIPYNIEFGQPQEVAAIRFLYVPPGKVEVIEIGQLTEII
                     GVMDFEITCKIGDEIVPIRSNFDRVGWVLVKGANREETLDILEKVSQQILIRTV"
     gene            3986..4429
                     /locus_tag="B7C51_RS22770"
                     /old_locus_tag="B7C51_22765"
     CDS             3986..4429
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /locus_tag="B7C51_RS22770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_22765"
                     /product="Ldh family oxidoreductase"
                     /protein_id="WP_083041332.1"
                     /transl_table=11
                     /translation="MRETFPRTKTYTIESLHQKVQNRFEQAGLSEYQAREMSEQLLYAE
                     MRGISSHGLVRIKWVTEQLHKYPLKNVRLLLQNREAELYDADGVLGYLALNEVAEKQQR
                     FEGQTIKFIGIRNTYPTGALSHFSEKLAAKGWIVLMSSTSPAV"
     gene            4390..4980
                     /locus_tag="B7C51_RS22775"
                     /old_locus_tag="B7C51_22770"
     CDS             4390..4980
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /locus_tag="B7C51_RS22775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_22770"
                     /product="Ldh family oxidoreductase"
                     /protein_id="WP_083041333.1"
                     /transl_table=11
                     /translation="MDCINEQHIPRRVGLYGDDKGLVGTNPWTFTLPVETNYSGQVVVD
                     VSLSEITHGQCLKAVEAGQPLPNYAASQPGGLAINHPDDLWKEGNWNAILHPVGRDKGF
                     KPFGVMWSLHVMGSRMLGLDGVESHDTFILLLSPSMWEPIIPAYEVIKGFNEEVKLLSM
                     SKVAHVPGEGRMSRLKEQKDQITVTQEIVNMFD"
     gene            5001..5894
                     /locus_tag="B7C51_RS22780"
                     /old_locus_tag="B7C51_22775"
     CDS             5001..5894
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) phosphonates-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020861284.1"
                     /locus_tag="B7C51_RS22780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_22775"
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="WP_083041334.1"
                     /sec_met_domain="phosphonates (E-value: 1e-105, bitscore:
                     351.1, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates-like (E-value: 2.2e-134,
                     bitscore: 444.9, seeds: 13, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 4.5e-62, bitscore:
                     207.9, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVKETKASQLRKMLNGTKLILVAGAHDGLTAKLAERNGFHAVWAS
                     GLGISAIQTVPDASILTMTELLQAAIVMNDACNLPIIADCDSGFGNIHNVVRMVEKYEG
                     AGIAGVCIEDKVYPKINSFDNGQQKLVSIEDFCAKIRAAKATQKDSDFVLLARVEALIA
                     GLGQDEAYNRACAYAQAGADAILIHFKQKTVDEIAEFMSRWNLDVPIVLVPTKYPDITF
                     NELEFLGVRMSIYANQALRGSVSAIDSILKRIMEKGSTRDIEGEIASIEDIFELQDIPK
                     MKQKELKFRKPKNATT"
     gene            5980..7508
                     /locus_tag="B7C51_RS22785"
                     /old_locus_tag="B7C51_22780"
                     /pseudo=""
     CDS             5980..7508
                     /GO_function="GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076555.1"
                     /locus_tag="B7C51_RS22785"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_22780"
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDHRKLTESILQLIIQTSTNLTSDVRESLFKAKMEEEMGTRSSLV
                     LSTIYQNMEDARQHELPVCQDTGMITFFIHTPVGIDQIEIRECIRKAIAQATRIGKLRP
                     NSVDSITGLNTGNNLGINTPIIHFEQWRNDQIEIKVILKGGGCENKNIQYSLPIELEGL
                     GTAGRDLDGVRKCILHAIYQAQGQGCSAGFIGVGIGGDRTSGYECAKLQLMRSVDDANS
                     VGELANLEDYIMEKANQLDIGMMGFGGKKTLLGCKIGAVNRIAPSFFVSVAYNCWAFRR
                     RGVLIDPKTGNITKWQYGKKTETQTGGGTQHSSLGEAIVLNTPISKEDVRKLKVGDVVL
                     INGKMHTGRDNLHKYLIDHDSPVDLNGGIIYHCGPVMIRDAMAIGESMQLDLLHLAVKN
                     HIKQLLLRNLVFAPLLVKAAWAKKPSKL"
     misc_feature    6003..6015
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 19.8"
                     /tool="antismash"
     gene            7738..8972
                     /locus_tag="B7C51_RS22790"
                     /old_locus_tag="B7C51_22785"
                     /pseudo=""
     CDS             7738..8972
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003307605.1"
                     /locus_tag="B7C51_RS22790"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_22785"
                     /product="MDR family MFS transporter"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKLQSIHPIGLNIIIGTFFARLATSMSIPFLSIYLTATKGFSPSI
                     VGAIIGTSALIGVCTSFIGGTLSDRYGRKMIMISSIIVWMLVFIGFSFANDVLSFFILS
                     ALNGICRSFFEPASRALLSDLTKSENRLLIFNLRYAAINAGVAVGPIIGLQLGSAKSTI
                     PFVVAAFVYFLYMTSLIIQFIKYKLGGIREM"
     misc_feature    7773..7786
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.68"
                     /tool="antismash"
     gene            9262..9426
                     /locus_tag="B7C51_RS22795"
                     /old_locus_tag="B7C51_22790"
     CDS             9262..9426
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006286158.1"
                     /locus_tag="B7C51_RS22795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_22790"
                     /product="GapA-binding peptide SR1P"
                     /protein_id="WP_024094155.1"
                     /transl_table=11
                     /translation="MKEEKATPEMGIILCSRCGEIIDTLDTEKVSVFYSLCNEPSCLEA
                     ADTKEEALA"
     misc_feature    10088..10102
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.82"
                     /tool="antismash"
     misc_feature    complement(10732..10742)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
ORIGIN
        1 cagccggcat tcaatcagct atggcggaga accagtcttt agagaagttc ccggatctta
       61 gcaacctgcc caaggggctg aatcctgcta caatgcggca gattatttat tttatgcggc
      121 agcagaagaa ggcactgtcg gcggaggaag tagcggatgg tgtaggcatt gcacgggtga
      181 cggcgcgcag ataccttgac tatctggaga agatccgcca agtgaggctg gtaatttctt
      241 atggtggaat cggaaggccg attaaccgtt acgagtttta tctaagcaat cgtaaataaa
      301 aagtccaaaa agaccaaaaa caccaaaaga accaaaaaag gcggcagaaa tagactaata
      361 acccgtagca tgaagacgta cgggttattt ggcgtgttaa cggataagat ctggagggat
      421 caaaaaacag accgttcggc cagagtagac ctttttatta tattgctctg ctgtatgggt
      481 taaatgcggg tgaaactcta tccttgtctg ccggagaggg agaagagggc gcaaatgacc
      541 tggagcattt tagttttgta cctgtttagt ctatttttaa tccttattcc caaattgaaa
      601 tatatgaaaa attcacaata gatattatca aaaaaatttt tagaagtggt aaacagatca
      661 gattttatta tttatgtcaa ttacgtcaat aaagcagaat ctttgtcttt atttaaaggc
      721 tatagaagca gagattaaaa taaactcagc tacgatttca gaagattttc gtaaagagag
      781 atgtggcaaa gaagggtggt ttttttacgg aaaattgata caatgggacg agaggaaggg
      841 tctgttatct gtgaaggaaa caacggtttc caatgtcaga atggaaagga agagaaagat
      901 ggttcgtgtt ctatttgttt gcctcggtaa tatctgccgt tctcctatgg cggaggcggt
      961 ttttaaacat cttgtcaaag aggccgggct tgagaaagag attgaggttg attccgcagg
     1021 aaccggcgat tggcatatcg gacatgctcc gcatgaaggt acccgtaagc tgctggaccg
     1081 acaggggatt tcctatgaag ggatgaaagc cagacaggtt gccaaatctg attcggaaca
     1141 gtttgattac atcatcatga tggatgccaa aaatgtgaag gacgttagac ggatttttgg
     1201 cgattcgggt catcctaaaa tgttccggat gatggatttt gctctggacg aaaccgttcg
     1261 ggacgtgccg gacccttatt ttaccgggaa ttttgaagaa gtttacgaga tgctcttaaa
     1321 gagctgcaaa catctgttac agcaggtgat cgaggagtat catctatctc caaaacaata
     1381 agcgatcaat tataaataac ccttgtttgg tgactaaaac cagacaaggg ttttcttaaa
     1441 aaattttacg acagccacaa cattgaatag atatcgttta tgtttataaa aaaaggaata
     1501 gatgatatat ttcacaataa ttaatattaa aaggaggttt tcacatgcaa gggcatatac
     1561 ttttttttaa cgctacttat ataaagaaag agttaatcct tcgaacggcc aaagatttgg
     1621 gactcacggt ttctgttgta ggaccaaagc tgcctaaatg gtctaaaaga tatgttgact
     1681 atttcattga aactaataca tacaatattg agcagacact ttctgtgtta agaaaatatc
     1741 acaagaaacg tccatttaat ggtgttatca ctttctggga tcgtgacgtt gagccagttg
     1801 cccatgtagc caatgaattt aatttgccag ccagttccaa gacagcagcg gcacgtgcac
     1861 gaaacaagtc actaatgcgc aaagcgctta agttaagaaa tgtcccacat ccaaaattca
     1921 tccacatctc tggttcttgg acagagttac aacaggcagc tgaaaaaatc gggtacccgt
     1981 tgatatacaa acctgtcggc gcttccgcct ccaaaggtgt atttaaggta gaatctgctg
     2041 accaactccg tcgaacttgg gaaaagatga tgaaacatgc aactccggaa aacgataaaa
     2101 tgtttacatt ctactgtgat gaatatctag tagaggaatt tatatctggt aaagaagtga
     2161 gtgtggaggg cgttgtcgct aagggaaaga tacatattgt aggtattaca gaaaaatggg
     2221 cggattccat aaattttacc gaataccagc atgcttttcc agcacgtctt ccagaagcgg
     2281 ttgtagagga tatatataaa gtaactcgca aaggcataga tgctctagaa ctagataact
     2341 gtggtttcca tgcggaactg ataattaccg atcacggcag taaaattata gaagttgcgg
     2401 gtcgtcttgg tggagacttt attactactc atcttatccc gctggcttac ggttctaata
     2461 tcacacgtgc taatattctg acggttttgg gagaagactt tgacctgagg cctaccaaaa
     2521 aacgcggtgc ttgtatccgt ttcttgctgg ctgaaaaggg gggaacagtc cgtcaatgga
     2581 tgggaacaga aaacgtgatg aacattccgg gtgtcgttca tttcggattt gataaacaag
     2641 taggcgatac ggtcgtgatg cccctgaaaa atttatggac ttccgtcttt gttacgtgat
     2701 agtagaaggt gatgataccg atgacgccat tctaaaagcg gaacaagcat tagccaatgt
     2761 gaggtgtatg attgaatgag ccgaaagaga ttattcttta tagggattcc tgaaactcat
     2821 gtgaaacgaa cattatccag atgtaaaaaa ttgggttatg agattattct tggtgatacc
     2881 aaaaataacc ttaaattaca tgccgaccgg ctagttataa ccgattttag ggatgagaaa
     2941 aacctgaggg atgtgacttg ttccttacaa gaggaatctc cccttgatgc catttttaca
     3001 tttaaggagt ttggattgat caatacatcc aaactactcc atgaatacgg cttgcgtgga
     3061 aaatccaacc gaagtaatta attcatgtaa caataaattc actactcgta acttattatg
     3121 ggaagcaggt cttttgagcc cggattatga actttgtgaa agtcttgaag atgcgaaaaa
     3181 actattgaaa cgaatagaag gtcctattat tatcaagccg cacaatctac agggaagcat
     3241 aggagtctat aaagtagaac aggaagagga tctgcaagtt ttgtatgaaa aatgcttgac
     3301 gcattgtaat gaaccgatag taatggcaga agaatttatt gttggacaag aagtttccat
     3361 tgaagccatt gtgtatcgtg ggaaaacaat aatatttggt gtaacagaaa aattactata
     3421 cccaggaaca tttgtcgaag ctggacatat ttctccgtat gctggtaccg aaatgagccg
     3481 ggaagaatat acaaaattgg tggaacggat cgtgggggca atgaaaatcc agatgggccc
     3541 tcttcatatt gaagggttcc atactgagaa aggtttttac gttggggaag ttcatactcg
     3601 ttatggtgga gataatatta cgacactgac tgaactcgca cttaagtgtg acatgacttc
     3661 tccgatattt gctgaattgg gtgacatccc atacaacatt gaattcggtc aacctcaaga
     3721 agtagcagcc attcgtttct tatatgtacc gccaggcaaa gtggaggtca ttgagatcgg
     3781 gcagcttacc gaaatcatag gggttatgga tttcgagata acatgcaaaa ttggtgatga
     3841 gatcgttcca atcagatcaa actttgaccg tgtaggctgg gttcttgtca aaggggctaa
     3901 tcgtgaggag actctggata ttctggaaaa agtatcacaa cagattctaa tacgaacagt
     3961 ttagtagtaa ctgaaaggag acattatgag agaaactttc cctagaacca aaacgtatac
     4021 aatcgaatcc cttcaccaaa aagttcaaaa tcgatttgaa caagcaggac tttctgaata
     4081 ccaggcccgt gaaatgagtg aacagcttct ttatgcggag atgcgtggta tcagttcaca
     4141 cggtcttgtc cggatcaaat gggtaacaga acaacttcat aaataccccc ttaagaatgt
     4201 ccgtttgctt ttacaaaacc gggaggccga actgtacgat gctgatggtg tacttggtta
     4261 tctagcttta aacgaggtag ctgaaaaaca acaacgtttc gagggtcaga cgatcaaatt
     4321 tatcgggatt cgcaatacgt accctacagg agctttatcc catttttctg agaagctggc
     4381 agcgaaagga tggattgtat taatgagcag cacatccccc gccgtgtagg tctttatggt
     4441 gatgataaag gactagtagg tacaaatcct tggacattta cgcttcctgt cgagacgaat
     4501 tacagtggac aagtagttgt tgatgtctct ctctcagaga ttactcatgg ccaatgtcta
     4561 aaagctgtag aggcgggaca acctctccct aactatgctg cttcccaacc gggtggttta
     4621 gccatcaatc atcctgacga tttgtggaaa gaaggtaatt ggaatgctat cttgcatcct
     4681 gttggccggg ataaaggctt caaacctttt ggagtaatgt ggagccttca tgtaatgggt
     4741 tcacgcatgc tcgggcttga tggagtagag tcacatgata ccttcatatt gttgctatca
     4801 ccgagtatgt gggagcctat aatccctgcc tacgaagtta ttaaaggatt taatgaagaa
     4861 gtcaagcttc ttagtatgtc caaagtggca catgtaccag gagaaggacg gatgagccgc
     4921 ttgaaagaac aaaaagacca aatcaccgta acacaagaaa tcgtaaatat gtttgattga
     4981 ataatgagga gggtgaggat atggtaaagg aaacaaaggc tagtcaatta aggaaaatgt
     5041 tgaatgggac caagcttatt ctggtagctg gtgcacatga tggtctcaca gctaaacttg
     5101 cagaacgcaa cggtttccac gctgtctggg cgagtggact gggtatctcc gctatccaaa
     5161 ccgtaccaga tgcgagtata ttaacaatga ctgaattgtt acaggcagca attgtaatga
     5221 acgacgcatg caaccttcca atcatcgccg actgtgactc cgggttcggt aatattcata
     5281 atgtggtgcg catggtagag aaatacgaag gtgcaggaat tgctggcgtg tgtattgaag
     5341 ataaagtcta tccgaaaatc aactcttttg ataatggaca gcagaaactg gtttcaattg
     5401 aagatttttg tgccaaaatt cgtgctgcta aagcaaccca gaaagattca gatttcgtat
     5461 tattggcccg cgttgaagca ctgattgccg gtctgggtca ggatgaagca tacaatcggg
     5521 cttgtgccta tgcgcaagca ggagcagatg ctattttgat tcacttcaaa caaaaaacgg
     5581 tagacgaaat tgcagaattt atgagtcgtt ggaatctgga tgtaccgatt gtactggtgc
     5641 cgactaaata ccctgatatt acatttaatg agttggaatt cctgggggta cgtatgtcaa
     5701 tctacgctaa ccaggctctt cgtggatccg tttcagctat agatagcatt ttaaaacgta
     5761 tcatggaaaa aggatcaacc agggatatcg aaggggagat tgctagtatt gaagatattt
     5821 ttgaacttca ggatatcccc aaaatgaagc agaaagaatt gaagtttcgt aaaccgaaaa
     5881 atgctacaac ttaaatattc tatagtggac aaatttggaa tattatgtat cataagcctc
     5941 gtacgaaaat atcatcattt ttaatcgggg tgtacaatta tggaccatcg caaactaact
     6001 gaaagcatat tgcaattgat tatccagaca tccacaaatc tcacttcaga tgttcgtgaa
     6061 agtttgttta aagcaaaaat ggaggaagag atgggaacgc gttcctcttt agtactctct
     6121 actatctatc aaaacatgga ggatgcccgg caacacgaac tccctgtttg tcaggatacc
     6181 ggaatgatta cttttttcat tcatacaccg gtcggtattg accagatcga aatccgtgag
     6241 tgcatccgga aagctattgc ccaagcaacc cgaataggaa aattacgtcc caattctgtt
     6301 gacagcatca cgggattgaa taccggaaat aatctgggga taaatacccc gattatccac
     6361 ttcgaacaat ggagaaatga tcaaattgaa attaaagtaa tccttaaagg tggaggttgc
     6421 gaaaataaga acattcagta ctcattacca atagaacttg aaggactagg tactgccggt
     6481 cgtgacttgg atggtgtacg taaatgcatc ctgcatgcca tataccaagc acaaggacag
     6541 ggttgttccg caggatttat aggagtgggt attggcgggg accgtacaag tggctacgag
     6601 tgtgccaagc ttcaattgat gcgatctgtt gatgatgcga attcagtagg ggaattggct
     6661 aatttagaag actatattat ggaaaaagcg aaccaactgg atattggtat gatgggtttt
     6721 ggcggtaaaa agactttact cggttgcaaa atcggtgccg taaaccgcat agctccttcc
     6781 tttttcgtat ccgttgcgta taattgctgg gcatttcgac gtcgtggtgt tcttatagat
     6841 ccgaaaacag gtaatataac caagtggcag tatggaaaaa agactgaaac tcaaacagga
     6901 gggggaaccc agcattcttc tttgggtgaa gcaattgtac tcaatacccc aatatccaaa
     6961 gaagatgtca ggaagttgaa agtaggtgac gtggttttga tcaacggtaa aatgcataca
     7021 ggaagggaca acctgcataa atatctcata gatcatgatt cccctgttga tctaaacgga
     7081 ggtattatct atcattgcgg cccggtcatg attagggatg cgatggcaat tggagagtcc
     7141 atgcagctgg acctactaca tctagccgtg aagaaccata tcaagcaact attattaaga
     7201 aatttggtat tcgcgccgtt gttggtaaag gcggcatggg cgaaaaaacc ctccaagctt
     7261 taagtgataa tggctgtatt tacctcaacg ctatcggcgg tgccgcacaa ttctacgcga
     7321 agactttcaa aaaagtcaac ggtgttcatt tcctggatga gttcggtgta ccggaagcaa
     7381 tgtggcattt cgaaacggaa ggattcattg ctatcgtcac catggatgcc catggcaatt
     7441 ctttgcataa agatattgag gaacaatcca gaaaaaaact tagtgatttt aaagaacggg
     7501 ttttctaaat tagatttatg aatgaaactg ttggcctgtg actttgctaa ttacatttag
     7561 attagcaggt catgggccga tattttttaa taactatgaa taccttctga ctgttactaa
     7621 tagctgcttt attcatatat gacctatcca aatagacgag cggtatatca acaatcattc
     7681 ctgcatacgc tttatcattc cacgtaatat aaacgaaaga tgggtgatga tttcaatatg
     7741 aaactgcagt ccattcatcc aattggacta aatataatta ttggaacttt ttttgcccgg
     7801 cttgctacgt caatgagtat tcctttttta tctatttatt taacagcaac taaaggattt
     7861 tctccgagca tagtaggggc tattattggt acaagcgctc tcataggagt atgtacaagc
     7921 tttattggtg gaactttatc agaccgatat gggcgaaaaa tgataatgat aagctctatc
     7981 atagtatgga tgctcgtatt tatcggattt tcatttgcca atgatgtatt atcttttttc
     8041 atattaagtg cattgaacgg gatctgtcgt tcattttttg aaccagcgtc aagagcactg
     8101 ttgtcagatt tgacaaagtc agaaaatcgg cttttaatct ttaatttacg ttatgcggct
     8161 attaatgctg gagtagcagt tggcccaata attggtttgc agctaggaag tgccaaatcg
     8221 actataccat tcgtagtagc ggcatttgta tattttcttt acatgacttc cttgattatt
     8281 caatttataa aatacaaatt aggggggata agggaaatgt aaaagaacga gtgacaataa
     8341 aagattctat tcacatcctc aggaaagata tggtgtttct atcggctctg attggtatca
     8401 ttttgggcta ttccggttac tcacattttt cttctagttt atcacaatac tttgctaatt
     8461 ctgatgtctt tcaggatggc gtatcgttgt tttctcatgc actcgttctt aatgccgtta
     8521 cggttttaat tatacaatat cctgttacac aaatcggcaa gaaatactcc gctgtagttt
     8581 cgataatgtt tggaacattt ataataagct taagtttgat tggttttgga atcatacatt
     8641 ctatttggat ggtctatgca tgcactattc tgtttacaat cggagaagtg cttattttct
     8701 caatgacgga tttgtttata gaccaaatcg ctattcaaaa tttgaagggc acttattttg
     8761 gtgcaatggg tttttcaagt tttgggggcg taatagggcc ttggttaggc ggaattcttt
     8821 tcgattatta cggatataac agtggtgggc ttgtatttat tatattatcc ggcatatgcg
     8881 cacttggagt tcccgtttta atttttgtta aattgctgtt aaatcgtagg gtgaatcaaa
     8941 aagtaacaaa tgttgaaaaa gttgtgagat gaatcaattt cgtaaattaa aaagacttgt
     9001 tttttaagca taaaaacggg aaaacttctc tatcgctaaa agatcgaaat ttcctccaga
     9061 catactgctt ggatgacttt atcagtccag atgtgtaaaa aagaatgact aggggtttat
     9121 aagaggtgct aatagaacga atatgatgtt ttttattaaa ttaacctcat atatattgtg
     9181 tactaaataa aaatatggta tgtattatat agagagaacg catttactct cgtacctaaa
     9241 ccatttcttg gaggcgggaa gatgaaggaa gaaaaagcaa caccggagat gggaatcatt
     9301 ttgtgcagcc gttgcggcga aattattgac acattggata cagagaaagt aagcgtcttc
     9361 tatagtctat gtaatgaacc cagctgtcta gaagctgctg atacaaagga ggaagcatta
     9421 gcgtgatcaa gaattcatgg gtcgttccat tcgaagcagg ccacgcaggt atgaaacaac
     9481 tactgggtgg taaaggtgcc aatttagccg aaatgaccaa ggcgggactt cctgttccgc
     9541 ctggttttac cgtaactaca gacgcatgcc gggcttttta cgagaacgga tcccagcttc
     9601 caggcggttt gtttgaagaa attttggatc aattaacagc tttggaacag gccaagcgcc
     9661 aaacttttgg cggcagggac aaccctttac tcgtctccgt acggtccggt tccgtcactt
     9721 ccatgcccgg tatgatggat accatcttga atctggggtt gaatgacgaa acggtccagg
     9781 ggctggccga gcagacggga aatccccgtt ttgcctatga ttgctaccgc cgtctgattc
     9841 aaatgttcgg aacggtcgtg taccagatgg aaagtatatc gtttgaacat cttcttgctg
     9901 atttgaaaaa aagagaacaa gtttccgagg atcataagct tcaagccagt gccctgcttg
     9961 aattaatcga acaatttaaa gcgctgatcg ttcgcaagac gggcaaaagt ttccctcaat
    10021 ccgttcaaaa acagctgtat ttgtctgtcg aggccgtttt ccggtcctgg aataaccagc
    10081 gggcccaagt ttacagaaaa ttatacagta tccctgatga acaggggaca gcggtcaaca
    10141 ttcaatccat ggtatttgga aatatgggga tgaacagcgc cacaggtgtg ttgtttacac
    10201 gtaacccctc cacaggttcc aaggaattat acggcgaatt tctgatgaat gcccaagggg
    10261 aggatgtggt tgccggaacg agaactcctg cccccatatc tgagcttgag accctccagc
    10321 cggacttgta tcagagatta atggaaacgg cgagacagct tgaatcccat tatagggata
    10381 tgcaggatat cgaatttact attgaagaag gaagattgtt cgtccttcag acccggaacg
    10441 gaaaacggac tgcccaggct gctgtaaagc ttgctgtcga tttggcgggt gaaggggtca
    10501 ttaacaagga ggaagctgtc tgccggatcg aaatggaaca cctggatcag ctattgcatc
    10561 gggtgatcga cccggaagca gaggtggatg tactggcgag aggacttccc gcatccccgg
    10621 gggcaggatc cggccaggtg gttttcgacg cggatactgc ggatgcatgg gccaaggaag
    10681 gccggcaggt catcctcgcc agcacggaaa caacaccgga agacattcac ggtgtcattg
    10741 cagccgaagg cgtgctgaca acccgcggcg gcatgacgag ccacgccgct gtcgtggcgc
    10801 gcggcatggg caagccgtgc gtatgcggct gtgaggcggc tgttgtagac gcctctaagc
    10861 gtgagcttcg cacgggcgga cgcaccgtgt gcga
//
