LOCUS       NZ_CP020557            10336 bp    DNA     circular CON 24-JUL-2024
DEFINITION  Paenibacillus larvae subsp. pulvifaciens strain SAG 10367
            chromosome, complete genome.
ACCESSION   NZ_CP020557
VERSION     NZ_CP020557.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus larvae subsp. pulvifaciens
  ORGANISM  Paenibacillus larvae subsp. pulvifaciens
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 4666454)
  AUTHORS   Dingman,D.W.
  TITLE     Paenibacillus larvae genome sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4666454)
  AUTHORS   Dingman,D.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2017) Entomology, CT Agric Expt Station, 123
            Huntington St, New Haven, CT 06504, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2016
            Assembly Method :: SMRT Pipe HGAP assembly v. February-2016
            Assembly Name                     :: sag10367-hgap-022916
            Genome Coverage                   :: 214.2x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002082155.1-RS_2024_07_24
            Annotation Date                   :: 07/24/2024 02:27:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,275
            CDSs (total)                      :: 5,166
            Genes (coding)                    :: 4,634
            CDSs (with protein)               :: 4,634
            Genes (RNA)                       :: 109
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 532
            CDSs (without protein)            :: 532
            Pseudo Genes (ambiguous residues) :: 0 of 532
            Pseudo Genes (frameshifted)       :: 400 of 532
            Pseudo Genes (incomplete)         :: 184 of 532
            Pseudo Genes (internal stop)      :: 26 of 532
            Pseudo Genes (multiple problems)  :: 72 of 532
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:07:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2803440
            Orig. end                         :: 2813776
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020557.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Dr. Adriana Alippi,
            CIDEFI-CIC, Facultad de Ciencias Agrarias y Forestales, Universidad
            Nacional de La Plata, 1900 La Plata, Argentina.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10336
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="13"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10336
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10336
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5336](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5336)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(113..122)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     gene            158..1705
                     /locus_tag="B7C51_RS14970"
                     /old_locus_tag="B7C51_14965"
     CDS             158..1705
                     /EC_number="2.3.3.13"
                     /GO_function="GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HMGL-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1271: 2-isopropylmalate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010499555.1"
                     /locus_tag="B7C51_RS14970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_14965"
                     /product="2-isopropylmalate synthase"
                     /protein_id="WP_083040513.1"
                     /sec_met_domain="HMGL-like (E-value: 2.1e-94, bitscore:
                     314.1, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKIYVFDTTLRDGEQSPGVNLNTQEKVEIGLQLEKLGVDRIEAG
                     FPAASLGDLEGVAAVAKAVRNASIIGLSRSREQDIDTVREALRDAQDPCIHVFLATSPI
                     HRQYKLRMDKKQVLETAEAAIRYAKKYFSKIEFSPEDAGRTELDFLCEVTEMAIRAGAT
                     VVNIPDTVGYMSPAEFGHIFATLRAQVSGIDKIQLSAHCHNDLGMATANALAAIMNGAD
                     QIEGTINGIGERAGNTSLEEVAMALETRQDLYQAKTSLVLNEIYRTSRLVSRLAGMAVP
                     GNKAIVGANAFAHESGIHQDGMLKEKTTYEIMSPETIGLKESKLVIGKHSGRHAFREKL
                     VDLGYVLNEDQLNTAFAKFKKLADKKKNVLDEDIRAIIDEKLIQVPEVFVLDTMQVSYG
                     NQSVPTATVRILTQDGGKLEEAAVGNGSVDAIYNAIDKATREEVELADYKIHSVSQGQD
                     ALGEVHVMLKQYGYSVLGRGVSTDILEASAKAYIDAVNRLFDKRSSHKTDRSNVTLI"
     gene            complement(1795..2028)
                     /locus_tag="B7C51_RS14975"
                     /old_locus_tag="B7C51_14970"
     CDS             complement(1795..2028)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151978.1"
                     /locus_tag="B7C51_RS14975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_14970"
                     /product="DUF5970 family protein"
                     /protein_id="WP_024095284.1"
                     /transl_table=11
                     /translation="MGSNFKHHVYLVGIPVILFVGYLISLTCEFLLPLLTFGLAGLYLF
                     VFAPVQNKIAKSLFLLIFILNLLGSIALYLNI"
     gene            complement(2041..2568)
                     /locus_tag="B7C51_RS14980"
                     /old_locus_tag="B7C51_14975"
     CDS             complement(2041..2568)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015418130.1"
                     /locus_tag="B7C51_RS14980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_14975"
                     /product="stage II sporulation protein M"
                     /protein_id="WP_083040517.1"
                     /transl_table=11
                     /translation="MIKTITVSLRMSKGPILASIIFFAVGFIIGLFSKEGFSVTAYASS
                     TNVLELLKNNVTVCLLMASGFFSFGLLSMFYLMSNGFILSGSIIESLQNGVPLYQIGFL
                     LLPHGIFEIPALLLSGSIGFKSLEMIWKLCFANTKGVLLDLFKHMMILTLLIGCLLVTA
                     SIIEIYVTPLFK"
     gene            complement(2565..3266)
                     /locus_tag="B7C51_RS14985"
                     /old_locus_tag="B7C51_14980"
     CDS             complement(2565..3266)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007408713.1"
                     /locus_tag="B7C51_RS14985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_14980"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_083040520.1"
                     /transl_table=11
                     /translation="MIKIKHLHKTYGHKVVLKDISFEVKKGSTYALLGTNGVGKTTTLK
                     IISGLLKPTAGTVSIDGNEQSHSLNELSANIGFVPDHAVLYEYLTGMEYLRFVGEMFQV
                     PSKDTNVYINEMLQKLHLFHEGDQLIKTYSHGMKQKLSIASALIHKPKVLILDEPLTGV
                     DLISGKAIRTLLKEYVDRGNTVLLSTHLLELAHSLCDTIGVIHDGEFVREFRSCEYSLN
                     HMEQLIENIYA"
     gene            complement(3259..4284)
                     /locus_tag="B7C51_RS27610"
     CDS             complement(3259..4284)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B7C51_RS27610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_230460622.1"
                     /transl_table=11
                     /translation="MYYVLSDGNMVQNIKYFLASPLFKQIFTYTPYVWIANHEHDMLTG
                     FLSFTVGIFVIMILFYIWSATLKRLDLIPYTGISDVTRAPKIRLNDQSKIFRAIFQKDI
                     LFLLRIHGFFARNFGNMLYALMVFLGFGIPFIQNQFSTYPEIAIVSFSVLIAHFTYLLV
                     GDALKMVLSVDGELKNQHLFQPNIKNAWQMVMPKISIYNLIVLLFSVFMSVLLAFLFKC
                     SVPLIICLFVMFATSGFLYGLVYISSTALYPKKNWEHDYEIGESGKAQTFTHLYSGFIF
                     IVSLQITGVTAYMMYTKSEIQTTVLNTASAGFILFTLLNYVIMFFYLKKINLFERIANH
                     D"
     misc_feature    complement(4643..4656)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.69"
                     /tool="antismash"
     misc_feature    complement(4644..4657)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.84"
                     /tool="antismash"
     gene            complement(5001..5336)
                     /locus_tag="B7C51_RS14995"
                     /old_locus_tag="B7C51_14990"
     CDS             complement(5001..5336)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151973.1"
                     /locus_tag="B7C51_RS14995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_14990"
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="WP_083040524.1"
                     /sec_met_domain="TIGR03651 (E-value: 6.2e-20, bitscore:
                     69.5, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNLVKSNKKVIVLLGLGLVSASLLYMFLLTGTNLGVTPVQGFSGN
                     AELASTLGISTAAAKKAIDIIDAASTIASIISLIGIVTGAGAVSYAIVATAKTMIKKYG
                     KKYAAAW"
     gene            complement(5383..5943)
                     /locus_tag="B7C51_RS15000"
                     /old_locus_tag="B7C51_14995"
     CDS             complement(5383..5943)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014470922.1"
                     /locus_tag="B7C51_RS15000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_14995"
                     /product="YIP1 family protein"
                     /protein_id="WP_083040526.1"
                     /transl_table=11
                     /translation="MIEKISNKKLVFLAVISILLSAILFQVSIYEKVLEIYHSSAKQHD
                     LPDIDGDIIQIVVIAIQGFSVLAIFIELLIGGFILYLIGFFLGSKKPKKTYLLLYTLTT
                     LVTSFKMLVMATVNVFTNDPSLIYSLKGSGLYLFDPFIILSTIILYFLSGKLTDLNKNK
                     RVVLAFSFLILKILLITFSTGGE"
     misc_feature    complement(6199..6216)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.23"
                     /tool="antismash"
     gene            6295..7383
                     /gene="leuB"
                     /locus_tag="B7C51_RS15005"
                     /old_locus_tag="B7C51_15000"
     CDS             6295..7383
                     /EC_number="1.1.1.85"
                     /GO_function="GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="leuB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005548621.1"
                     /locus_tag="B7C51_RS15005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_15000"
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="WP_083040528.1"
                     /transl_table=11
                     /translation="MADAKKIAILAGDGIGPEVVAEAEKVLKRTEELFGYRFETEHGIF
                     GGIAIDKKGTPLPQDTLKLCQQADAVLLGAVGGPKWDNNPKDLRPETGLLGIRKALGLF
                     SNIRPAVVFDCLKEASTLKPEVLEGTDLIVVRELTGGIYFGEKLRRNSENGQEAVDTCV
                     YNVQEVERIVRQAFNIARTRRKKLASVDKANVLETSRLWRETVNRVSADYPDVELEHVL
                     VDNCAMQLLRRPASFDVIVTENMFGDILSDEAAMLTGSIGMLSSASLGEGSFGLYEPVH
                     GSAPDIAGQGIANPIATILSVALMFRLTFGYHEAADCIERAVKEILDAGHRTGDIAIDR
                     SKALGTAKMGDLIIGVMKKHNK"
     misc_feature    complement(7500..7513)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     strong, score: 28.14"
                     /tool="antismash"
     misc_feature    7501..7514
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 25.44"
                     /tool="antismash"
     gene            7608..8153
                     /locus_tag="B7C51_RS15010"
                     /old_locus_tag="B7C51_15005"
     CDS             7608..8153
                     /EC_number="1.11.1.-"
                     /GO_function="GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1196: alkyl hydroperoxide reductase/ Thiol specific"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018975434.1"
                     /locus_tag="B7C51_RS15010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_15005"
                     /product="peroxiredoxin"
                     /protein_id="WP_024095277.1"
                     /transl_table=11
                     /translation="MAERLVGKKAPDFTMETAHGDGTGFGKVSLSDYKGKWLVFFFYPL
                     DFTFVCPTEITALSDAAAQFRDLNTEILAVSTDSIHSHKAWINAPKNDGGLGKVNFPIA
                     SDITKSVARDYGVLIEEEGVALRGLFIINPEGELKYQVVNHNDVGRSVEETLRVLQALQ
                     SGGLCPMNWKPGDKNLEV"
     gene            8298..8693
                     /gene="gcvH"
                     /locus_tag="B7C51_RS15015"
                     /old_locus_tag="B7C51_15010"
     CDS             8298..8693
                     /GO_component="GO:0005960 - glycine cleavage complex
                     [Evidence IEA]"
                     /GO_process="GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /gene="gcvH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009672168.1"
                     /locus_tag="B7C51_RS15015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_15010"
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="WP_023484877.1"
                     /transl_table=11
                     /translation="MMDMPAHLLYTPDHLWVRIDGNRAMIGITEYAQEELGIVVFAELP
                     PLGEKVRTGLYMSMVESAKQDMDIIAPVSGTVVLVNQALASNPMHINTSPYEYGWILVI
                     EMSRPEEMEKLWDAKTYQEAYATCRGE"
     misc_feature    9298..9312
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 20.41"
                     /tool="antismash"
     misc_feature    9454..9470
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 22.69"
                     /tool="antismash"
ORIGIN
        1 aacttcattc tggagcaaac aaacggagcc ttcttgacag caacacgccg caaggaagca
       61 gaacacccgc ttgagcaggt aggtaaagaa cttcgcgaaa tgatgcactg gatcaaaaaa
      121 taggtgaaaa cagaacaata tagaaggagg ggacaggatg aggaaaatct atgtatttga
      181 taccacattg cgggatggtg aacaatcacc cggcgttaac ctcaatactc aggaaaaggt
      241 ggaaatcggc ctccagctcg aaaagctggg cgtagatcgg atcgaagcag gatttcctgc
      301 agcttcccta ggcgacctgg aaggtgtggc agcagtggct aaagcggtcc gtaacgcttc
      361 gatcatcgga ctgtcccgtt ccagggaaca ggatattgac acagtaaggg aagccctgcg
      421 tgatgcacag gacccttgca tacatgtatt tttggccact tcccccattc atcgtcagta
      481 taaattgcgc atggataaga agcaggtgtt ggaaacagct gaggcggcca tccgttatgc
      541 gaagaaatat ttcagcaaga ttgagttctc tccggaggat gccggacgga ctgaacttga
      601 ttttctttgt gaagttacgg aaatggcgat tcgggcaggc gcgacggttg tgaatattcc
      661 ggatacggtt ggttatatga gcccggcaga gttcggacat atattcgcca ctctgagggc
      721 acaggtttcg ggcattgata agatccagct tagcgcacat tgccataacg atctgggtat
      781 ggcaacggct aacgccctgg cagccattat gaacggggct gaccagatcg aaggaacgat
      841 caatggtatc ggcgaacggg cgggaaatac ctcactggaa gaagtggcca tggctctgga
      901 gacccggcag gatctgtatc aagccaagac ttccctcgtg ctgaatgaaa tttaccggac
      961 cagccgccta gtcagccggt tggcgggtat ggcggtaccc gggaataaag caatcgtggg
     1021 agccaacgct ttcgctcacg aatcagggat tcaccaggac ggcatgctaa aggaaaaaac
     1081 gacttatgaa atcatgtcgc ctgaaacgat cgggttaaaa gaaagcaagc ttgtaatcgg
     1141 taaacattcc ggccgtcatg cgttccgcga aaaactcgtg gatctgggct atgtactgaa
     1201 cgaggatcag ttgaatacgg catttgccaa atttaaaaag cttgccgata agaagaagaa
     1261 tgtactggat gaagacatcc gggctattat cgatgagaaa ttgattcagg ttcccgaagt
     1321 gttcgttctg gatacgatgc aagtttccta tgggaaccag tcggtgccta ccgctacagt
     1381 tcgtattctg acgcaagacg gcggtaaact agaggaagcg gcagtcggga acggttcggt
     1441 cgatgctatt tataatgcga ttgataaggc cacccgggaa gaagtggagc ttgccgatta
     1501 caagatccat tctgtatccc agggacagga tgcgcttgga gaagtgcatg ttatgctgaa
     1561 gcaatacggc tattctgttc tggggcgcgg ggttagcact gatattctgg aagcgagtgc
     1621 caaagcttat attgatgcag tgaaccggct gtttgataaa cgcagttccc ataaaacgga
     1681 tcgcagcaac gtcacactaa tttaaaatga tccgtctgtt cccaatgaaa aaccttttgt
     1741 tatcggagtg taaattctgg ggcaaaaggt tttttgctaa gtagaaatgc tgttttagat
     1801 attcaaatac aaggcaatac tgcctaagag attaaggata aagatcagca gaaagagaga
     1861 cttggcgatt ttgttttgaa caggtgcaaa tacaaacaga taaagccctg ctaaaccaaa
     1921 cgtcaacaaa ggcagtaaga attcacaagt taaagaaata aggtagccaa cgaagagaat
     1981 aacaggaatt cctacaaggt aaacatgatg tttaaaattg gatcccatcg aaatgtctcc
     2041 ttatttaaat aatggagtaa catagatttc tattatggat gccgtcacta ataaacaacc
     2101 aatcaaaagt gtcaatatca tcatatgttt aaataagtct aacaagactc cttttgtatt
     2161 tgcaaaacat aatttccaaa tcatttctaa acttttaaaa ccgatagaac cactcaataa
     2221 taatgctggg atttcaaaaa taccatgagg caagagaagg aaaccgattt gatacaaggg
     2281 cactccattc tggagactct ctataataga cccacttaaa ataaacccgt tagacatcaa
     2341 gtagaacatg gataggagtc cgaaagaaaa aaaaccggaa gccataagaa gacaaaccgt
     2401 gacgttgttt ttcagcagct ccagtacatt ggttgatgat gcataagctg taacggagaa
     2461 tccctctttc gaaaacaggc cgataataaa gccgacagca aaaaagatta tggatgccag
     2521 tatgggtccc ttactcattc tcagtgaaac ggttatagtt ttaatcatgc gtaaatgttc
     2581 tcaatcagct gttccatgtg gttcaatgag tattcacaag aacgaaactc ccgaacaaat
     2641 tcaccatcat gaataacccc gattgtatcg cacaaagaat gggcaagttc caacaagtga
     2701 gtagatagaa ggaccgtatt tccccggtct acatactcct ttaataacgt ccggatcgct
     2761 ttaccgctta taaggtcaac tcctgttaaa ggctcgtcaa gaatcaaaac cttcggctta
     2821 tgaattaaag cggacgcgat agataatttt tgcttcattc catgtgaata tgttttgatt
     2881 aactgatcgc cttcatgaaa taaatgtaat ttttgtaaca tttcattaat atatacattt
     2941 gtatcttttg agggaacttg aaacatttcc cctacaaagc gcaaatactc catacctgtt
     3001 aagtattcat agagaacagc atgatccgga acaaatccta tattagcaga tagttcattt
     3061 aacgaatggc tttgttcatt tccatcaatg gatacggttc ctgcagtagg cttaagcaac
     3121 cccgaaataa tcttcaaagt tgttgtcttt cccacaccat ttgtcccaag aagcgcatag
     3181 gtggaccctt tttttacttc aaaagagata tctttcaaga ctactttgtg cccatacgtt
     3241 ttatgtaaat gtttaatctt aatcatgatt ggcgatcctt tcaaacaaat tgattttctt
     3301 taaataaaag aacatgataa cataattgag caatgtaaaa agaataaatc cagctgatgc
     3361 ggtattcaat acagttgttt gtatttcaga tttggtatac atcatataag ccgttacacc
     3421 agtgatttgc aatgatacaa taaaaataaa tccactgtac aaatgagtaa aggtttgggc
     3481 tttccctgat tctccgatct catagtcatg ttcccagttt ttcttaggat atagggcagt
     3541 gctggaaata tagacgagac cgtataagaa accagatgtt gcaaacataa caaacagaca
     3601 aatgatcagt ggcaccgaac acttaaagag aaaagccaac aatacagaca tgaacacaga
     3661 aaatagcaga acgattaaat tataaataga aattttcggc atcaccattt gccatgcatt
     3721 ttttatattc ggttgaaaaa gatgctgatt tttcagctca ccgtctactg acagaaccat
     3781 tttcagtgca tcaccaacca atagataggt gaaatgggca atcagtacag aaaaagagac
     3841 aatagcaatt tctggataag tggaaaattg gttttggatg aaggggatcc caaaacctaa
     3901 aaataccatt agcgcataaa gcatattacc aaaatttctg gcaaaaaatc catgaattct
     3961 taacaaaaat aggatatctt tttggaaaat agcacggaat attttggatt gatcattcaa
     4021 cctaatctta ggtgcacggg taacatcact aataccggta tacgggatca aatctagtct
     4081 ttttaaggtg gcagaccaga tatagaacag gatcatgatc acaaagattc ccactgtgaa
     4141 agatagaaac cctgtcaaca tatcgtgctc atgattggct atccatacat atggggtgta
     4201 agtaaatatt tgtttgaaaa gcggcgatgc aagaaagtat ttgatgttct gaaccatatt
     4261 tccgtctgac agtacataat acaccccata ccccataagt agaggaaccg tcagactgat
     4321 gacaaacagg acggatgaaa taattttgga atgtgcaagc tgcctccaaa catggttcat
     4381 tatgtaagga atataagaaa agagcatggc aaataaacaa gtaaaggtga aataaatgaa
     4441 tcctataaga aagataaaca atgggtgaac agttgtctgt tgaatggcaa agagtattat
     4501 aaaaaccgga aacacaaaga ggtaccatac acatatactt ttgataacag attttaatac
     4561 aaaggcagaa aaaagttgta aaaaccggac ttcttctaat tgatggatga atttccgcgt
     4621 atccagcaag aaaatttttt tatttttttt gaaaaagtcc atcgtccaga gcagctgttc
     4681 gagaaagatc gcaatgagta taaactgact ccaggtattt agcggcagct caatgaaacg
     4741 tgttcccaac ataacaacaa gagttaacaa acataaataa actagtaaaa gggcaattcc
     4801 aatactatac catgttcttc ttccaactgt ttcaagattc cagtcatctt tcattttacg
     4861 aatggatgat aacgaattat gtttaataag agaaatgatc cgctttacta ttctgttcat
     4921 catttacttc attccttgtt tgtagtatgg gggagagcct tttatgcttt tatacagaaa
     4981 gtattacttt atgtttcagt ttaccaagca gcagcgtatt ttttaccata ttttttgatc
     5041 attgttttag ctgttgcaac aattgcataa gaaacagcac cggctcctgt tacaatacca
     5101 attaaagaga taatagatgc aattgttgaa gctgcatcaa tgatatcaat tgcttttttc
     5161 gctgcagcag ttgagatgcc taaagtagag gctagctcag cattgcctga aaagccctga
     5221 acaggtgtaa cacccagatt tgtaccagtt aatagaaaca tatataacaa tgaagcgctc
     5281 actaggccca aaccaagaag tacaatgact tttttgttag actttaccaa attcatttcc
     5341 caacatctcc aatctcataa atttataata aaatccagta ccttactctc cccctgtact
     5401 gaatgttatt aacaatattt tgagtataag aaaactaaag gccaggacaa ctcttttatt
     5461 tttattcaga tccgttaact tgccacttaa aaaatataga ataatagtgg atagaataat
     5521 aaatggatcg aacaaataga gtccgctgcc ttttaatgaa tagattaaac taggatcatt
     5581 cgtaaacaca ttaacggttg ccataacaag cattttaaaa gaagtaacta aagtggtaag
     5641 cgtgtaaagc aggaggtaag tttttttcgg tttttttgag cccaaaaaaa aaccaatcag
     5701 ataaagaata aaaccaccga ttaaaagttc aatgaaaatg gcaagaacag agaatccttg
     5761 gatggcaatc acaacaattt ggataatatc gccatctata tctggaaggt catgttgttt
     5821 ggcagaggag tgatagattt cgagtacttt ttcataaata cttacttgaa atagaatggc
     5881 agaaagcaaa atggagataa cagctaagaa aaccagtttt ttgttactta ttttctcaat
     5941 catatcatta agttttacct ccttattaag gaaatcgatg taaaacattt gcggctaaat
     6001 cgttaattaa agcgaaatga gtttctgaat ttatatattt ttacattaca ggtatatatt
     6061 tgtaattcta aggtgctgaa acggattagt caatatccag atgaaatatg tcttaagaca
     6121 tctcatcagg atacttaacc ttaatctggt ctttaggata ttccgggaga aaaagttctt
     6181 ttaaaggatt tataggttta tcctataaaa gtgatagctt ttatatctta gtcaactaat
     6241 ggcaaatatg atacaactag atatagcgaa gtaacaatgg ggagtgaacg agagatggca
     6301 gacgcaaaaa aaatagcaat ccttgcagga gacggtattg gccctgaagt ggtagcagaa
     6361 gccgagaaag tgctaaagcg taccgaagaa ctattcggat accggtttga aacggaacac
     6421 ggcatttttg gtggcattgc cattgacaag aaaggaactc cgctgcctca ggatacattg
     6481 aagctgtgtc aacaagcgga tgccgtgctt ctgggggcgg ttggcgggcc caaatgggac
     6541 aacaatccca aagatctgcg acccgaaaca ggactgctcg gaatccgtaa agccctcggt
     6601 ctgttttcca atattcgacc ggctgttgta tttgattgct tgaaggaagc ctctacctta
     6661 aagcctgaag tgctggaggg aaccgacctg atcgtggtcc gggaacttac aggcggaata
     6721 tatttcggag agaagctccg ccgcaattca gagaacggtc aggaggctgt cgacacttgc
     6781 gtctacaacg ttcaggaggt tgagcgcatc gtgcgccagg cctttaacat cgcacgcact
     6841 cgccggaaga agctcgcttc cgtagataag gccaatgtac ttgaaacctc gcgcctttgg
     6901 agggagaccg tgaatcgggt ttcagccgac tacccggatg ttgaacttga gcatgtactc
     6961 gtcgacaact gcgcgatgca gttgcttcgc cgcccggcca gctttgatgt cattgttact
     7021 gagaatatgt ttggtgacat tttgagtgat gaagcggcca tgctcaccgg ttcaatcgga
     7081 atgctctcct cagcctctct cggggaagga agctttggcc tctacgaacc cgttcacgga
     7141 tcggcccccg atatcgcagg gcaaggtatt gctaatccga ttgccaccat tttatcggta
     7201 gctctgatgt tccgtctgac ctttgggtat cacgaagcag cggactgcat cgagcgcgcg
     7261 gttaaagaaa tactcgatgc cggacaccgt acaggcgata ttgccataga ccgctctaaa
     7321 gcattaggaa cggcaaagat gggtgatctg attatcgggg tgatgaaaaa acataacaaa
     7381 taggggaaag aagaaggcct taaagtctcc accactttaa ggtctttttc ttttaaaacc
     7441 cttataccaa ctagatttaa atattagaat aaccattttc aaccaaaatt atggacgatt
     7501 tataataatt ataatataat acttgattta atattgactt tcttctaggc aaatgatacg
     7561 atcgaaacag attgaaatga accatttttg aaggaggaat tgtacaaatg gctgaacgtt
     7621 tagtaggcaa aaaagctcca gattttacta tggaaactgc acacggtgat ggtaccggct
     7681 ttggtaaagt atccctttcc gactataaag gcaagtggct ggtattcttc ttctacccac
     7741 ttgatttcac tttcgtttgt ccgactgaga tcactgcact cagtgatgcg gctgctcaat
     7801 tcagagatct gaatacggaa attctggctg tttcaacaga cagcattcat tcccataaag
     7861 cttggatcaa tgctccaaag aatgacgggg gtcttggaaa agtgaacttc ccgatcgctt
     7921 ccgatattac taagtccgta gcacgcgatt atggtgtcct gattgaagag gaaggcgtag
     7981 cccttcgcgg actgttcatc atcaaccctg aaggtgagct gaaatatcaa gtggtgaacc
     8041 ataatgatgt aggccgcagt gtagaagaaa cacttcgcgt tctgcaagcc ctgcaatctg
     8101 gtggtctttg cccaatgaac tggaaaccag gggacaaaaa cctggaagtc taagcttgta
     8161 agggataggc tgatttacgt acaggacgta tccttcattt ctataacctt ttttctgctg
     8221 cggcagggaa gaggtttttt catcttcttc ttcctgatat actcaatata agaggcaact
     8281 aggaagggga gttcttcatg atggacatgc ccgcgcattt gctttatact cctgatcatt
     8341 tatgggtaag aatcgacggt aaccgggcca tgattggtat tacggaatac gcccaggaag
     8401 agttgggtat tgtggttttt gcggagcttc ctccccttgg ggagaaggtt cggacaggcc
     8461 tctatatgag catggtggaa tccgcaaaac aggatatgga tattatcgcc ccggtatccg
     8521 gtacggttgt actggtcaat caggctttgg cgtccaatcc aatgcatatt aacacctctc
     8581 cctacgaata tggatggata ctggtaattg aaatgagccg tccggaagag atggaaaaat
     8641 tatgggatgc aaaaacctac caggaagctt atgcgacctg tagaggggaa taaaaaaatc
     8701 tggcgaggaa gaatcctttg ccagattttt tattttccta tagctttcag ttctttatat
     8761 ttagctgcat atttgtttac ggttaataca taagtagcat gggaccaggt taaaggggct
     8821 actgaaacgg gggccccgct ataagggtcc agctgttcag gcaggatgcc gctttgcatg
     8881 gcattgcgga ctacccattc cagagtttct tttggtttgt ctaactcact cagatttgaa
     8941 gccatttcag tctgccattc cgccatccac agagtacaga ttatccacgg atttccgggc
     9001 acctgctgga tatctgacga acgctggaag tagtaatcgt tataatagcg gctgatcccc
     9061 cgattggagt ttgaatagaa agtccccgca ggttagcttc catcgtccga attacacgat
     9121 catcatctgg cggaagtacg ccaaaggcaa aaatcccgta taccgagctt tccagtgtcc
     9181 gatctttatg ccattttccg tactccttaa ataatccacg tataaatctg cctgtctgaa
     9241 catcccaaag atgctccaga attccgcagc ggattttttc ggctgttccg tgataccggg
     9301 cccttcgttc ctcatccccg aaaaggctgc aaaagttacc tgcagctatt agcccgccat
     9361 atacggctga ggccgtaaaa gtataaatcc cgtaccgttc ttcccataaa tcgtaacttg
     9421 gcttgggcaa atgaagactt tcttctatat aactgcataa aaaacgggcg gctggccgaa
     9481 taagggtact gtataacgac tgggccagtt ccagattctc atgttgaaga tagtcttgcc
     9541 aaagggcgaa caaaaccagt cccgtttcat cttcctgaat cggaagctgt tcttcccctc
     9601 cgtgtacata aggatgccag ctggaaccaa cagttccgtc cggattatac ttatggagta
     9661 aatagccctc ccgggaaagc gccctggcgc agaaccggta gaacggttcc atcatgccgg
     9721 aataccctgc tttggacatg gcatgagcta taagagcacc atctcttggc cacatgtagc
     9781 tgtaatgatc ccggttgctt tgcataatgt cggagtcatt ggcagctatg atagctccgt
     9841 ttacatcggt ttgggaccgt acgatcagca aactttgctt ataaagacgg attatgtcat
     9901 cggtcaaatc ggcaaagtac cggtcggatt tattaaccca tcgctgccag tacacctgca
     9961 cccggttcaa aagtttgtcc ggtccgctct cacggacata ttgatccaga gagacgacct
    10021 cctccagatt ttggcctgct gtcatccaat aatatagttt tcctgatccg tgagaagcca
    10081 attttaaacg gaaactgatg gtactgtcta cagagccttg agctatgggg ttccccatta
    10141 agtatccgtc ctctgcatcc ctccacgtgc cttccgcatg ataaaaccgt ttaactcctg
    10201 ttgtatattg gtaaatgccc tcgcatccgg ttgttccgtt aaacatgaaa tatctgtttt
    10261 ttttatagtg gaaaatcgtg tggttggaag gatagtaggc tgcggtgtcc ccgacttccg
    10321 tttcgttaat ttgtaa
//
