LOCUS       NZ_CP020557            10327 bp    DNA     circular CON 24-JUL-2024
DEFINITION  Paenibacillus larvae subsp. pulvifaciens strain SAG 10367
            chromosome, complete genome.
ACCESSION   NZ_CP020557
VERSION     NZ_CP020557.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus larvae subsp. pulvifaciens
  ORGANISM  Paenibacillus larvae subsp. pulvifaciens
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 4666454)
  AUTHORS   Dingman,D.W.
  TITLE     Paenibacillus larvae genome sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4666454)
  AUTHORS   Dingman,D.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2017) Entomology, CT Agric Expt Station, 123
            Huntington St, New Haven, CT 06504, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2016
            Assembly Method :: SMRT Pipe HGAP assembly v. February-2016
            Assembly Name                     :: sag10367-hgap-022916
            Genome Coverage                   :: 214.2x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002082155.1-RS_2024_07_24
            Annotation Date                   :: 07/24/2024 02:27:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,275
            CDSs (total)                      :: 5,166
            Genes (coding)                    :: 4,634
            CDSs (with protein)               :: 4,634
            Genes (RNA)                       :: 109
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 532
            CDSs (without protein)            :: 532
            Pseudo Genes (ambiguous residues) :: 0 of 532
            Pseudo Genes (frameshifted)       :: 400 of 532
            Pseudo Genes (incomplete)         :: 184 of 532
            Pseudo Genes (internal stop)      :: 26 of 532
            Pseudo Genes (multiple problems)  :: 72 of 532
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:07:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2686434
            Orig. end                         :: 2696761
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020557.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Dr. Adriana Alippi,
            CIDEFI-CIC, Facultad de Ciencias Agrarias y Forestales, Universidad
            Nacional de La Plata, 1900 La Plata, Argentina.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10327
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="12"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10327
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10327
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5327](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5327
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            337..756
                     /locus_tag="B7C51_RS14275"
                     /old_locus_tag="B7C51_14275"
     CDS             337..756
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B7C51_RS14275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B7C51_14275"
                     /product="hypothetical protein"
                     /protein_id="WP_083040391.1"
                     /transl_table=11
                     /translation="MLGNVSVLDEYANYLAERPNDIPEGLLMITQAANAHGFSIDHILE
                     QFPEPSLENDVNVVRIEYHIEFYYQKGIYELNQQRFTTGLESILHCLSLSIPTKRHSIS
                     ILCAAQFEQYQNNASDPQREKFGNLMKEVLEVEKI"
     gene            1283..1903
                     /gene="cmr6"
                     /locus_tag="B7C51_RS14280"
                     /old_locus_tag="B7C51_14280"
     CDS             1283..1903
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /gene="cmr6"
                     /inference="COORDINATES: protein motif:HMM:NF015728.4"
                     /locus_tag="B7C51_RS14280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_14280"
                     /product="type III-B CRISPR module RAMP protein Cmr6"
                     /protein_id="WP_083040393.1"
                     /transl_table=11
                     /translation="MESNNLVAYLNNKEVSNFNNAPELRTSFIICLKDKIMQEFEDLVG
                     IPYIPGSSLKGVVRNCWLHEHLDGDLGLGENATRKVFFDKCFGTGADDEQLSSGKLIFL
                     DAYPVETYQIVKDVQTPHYKTYYNQKGSSPPRDDDSPNIIPLMAIEDTIFDIYIGMNFK
                     TDELDANLRNEISKTIISTLSEYGVGAKTAIGYGLGEVTMCDS"
     gene            2199..>2357
                     /locus_tag="B7C51_RS14285"
                     /pseudo=""
     CDS             2199..>2357
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /locus_tag="B7C51_RS14285"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="IS5/IS1182 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLRKTGYEGRSQISMVSLDELLPKDHLVRKIEYAIDFSFIYDLVR
                     DVYSEDNG"
     gene            complement(<2352..>3095)
                     /locus_tag="B7C51_RS14290"
                     /old_locus_tag="B7C51_14285"
                     /pseudo=""
     CDS             complement(<2352..3095)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014281001.1"
                     /locus_tag="B7C51_RS14290"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="B7C51_14285"
                     /product="IS256 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEMLEAELDTHLGYEKHEVKAKKTPNSRNGRSHKTVVSEYGEQQI
                     PVPRDRMGEFEPLVVKKHQSNVTGIEDQIVALYAKGVSTP"
     gene            complement(3219..3878)
                     /locus_tag="B7C51_RS14295"
                     /old_locus_tag="B7C51_14290"
     CDS             complement(3219..3878)
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016113361.1"
                     /locus_tag="B7C51_RS14295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_14290"
                     /product="response regulator transcription factor"
                     /protein_id="WP_083040395.1"
                     /transl_table=11
                     /translation="MEQTIQIMLVDDHALLRSGLKLLLQKKPPFKVVGEAANGIEALRL
                     YEDLRPHLLILDISLPRLDGIQVLREIKLRHEEAKVIVLTMHEDEDYITSIMQAGASGY
                     IPKAAVDEELYTAIDSVMNGYMYLRPKETKTLISSMLKKVPKEADSRNPYVILSPRERE
                     VLRFLVRGYSLAETAKALTVSIKTVDTHKTRMMNKLNISKKSELIEYALEYRLLIE"
     gene            complement(3847..>4731)
                     /locus_tag="B7C51_RS14300"
                     /old_locus_tag="B7C51_14295"
                     /pseudo=""
     CDS             complement(3847..>4731)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016113360.1"
                     /locus_tag="B7C51_RS14300"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_14295"
                     /product="ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="KPINNLVAVATGISAGNLSLRAKATSDDEVGKLAQAFNEMADNLI
                     SSNTEIEKLLKELQEKERLRDTLILKLLSAQEDERKRISRELHDETSQALTSLMVTMRV
                     LANEAKDAEQQELLNTSREIAASILREIRDLAVELRPPILDDMGLIPAIKKYIQKFEEK
                     YGIAVVLSAPEEDIAIDSRTAVALYRILRESLTNVVKHTAATRIVIGLRLADHCIQLTV
                     HDNGHGIQQEDFERARRQNRIGLYGMKERAELLGGSFFVRTTNIGGTELIVSIPLKLEK
                     GCSDGTDHSNHAS"
     gene            5001..5327
                     /locus_tag="B7C51_RS14305"
                     /old_locus_tag="B7C51_14300"
     CDS             5001..5327
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IId"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017358050.1"
                     /locus_tag="B7C51_RS14305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_14300"
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="WP_036655898.1"
                     /sec_met_domain="Bacteriocin_IId (E-value: 3.3e-23,
                     bitscore: 79.8, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVATRNEIKLSLLLGALAMGFLMLALFLFSLQFLPVADLAKEFGI
                     PASIAAVVLNIVNAGGAVTTIVSILLAVGSGGLSLLAAAGKETIRQFLKKKIQEKGKRA
                     TIAW"
     gene            5928..7160
                     /locus_tag="B7C51_RS14310"
                     /old_locus_tag="B7C51_14305"
     CDS             5928..7160
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B7C51_RS14310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B7C51_14305"
                     /product="hypothetical protein"
                     /protein_id="WP_235430722.1"
                     /transl_table=11
                     /translation="MYNIVVKKIGWIRLIFFSAVSSIFFMLGRLFISGFILPFYEAFRQ
                     NFHSIHSAVDNASWIQLSEQLVSLYSNQLHSIQVFLFLEGSTFLQVQQFISLPAATLLL
                     AAGVLGLLIIPAKWIPTEETNSTSHFKDLFHWYLRALHKVRYLIFRADLLIYKDLRVLQ
                     GKRWLLSKEFFNYSFISYEAVFYAGVFTGFLSAVESPALQVQLLFTMNVIIMANQALEM
                     RVLTPSLFSISAEKQNIWLYQLSLSSLFQFFRSKIKLFYCLFFFPSLFLTLFNFFMMLI
                     YEIYDVQMVILIIGIFIGFFIFPLIQLYLFPFVTKFNFIHEYEIGTTKEETGIVDKGQS
                     FIRSFLVMPFIYYLIVSSFFPVLQHSVKVVNVLSILYFSGVSFICWIYCHKMISKGIGY
                     VEKIKIYSISE"
     gene            7123..7683
                     /locus_tag="B7C51_RS14315"
                     /old_locus_tag="B7C51_14310"
     CDS             7123..7683
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342553.1"
                     /locus_tag="B7C51_RS14315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_14310"
                     /product="stage II sporulation protein M"
                     /protein_id="WP_083040396.1"
                     /transl_table=11
                     /translation="MLKKLKSILSANKLVCVLGFFYLGVFFLAVCTGSFTQAERIPHLE
                     DNNQASFLDIFLNNIQVSMAILGIGSITGGLMSAVILFYNGYIIGKLVQFLFSNDQAGA
                     LWTGLMPHTLLEILGLTLFAIVSSFPFISIYQFLIGRTIDVKQLILTNIRFILIGILLL
                     FVASLIEDYISYVQFILELKGER"
     gene            7686..8231
                     /locus_tag="B7C51_RS14320"
                     /old_locus_tag="B7C51_14315"
     CDS             7686..8231
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017358053.1"
                     /locus_tag="B7C51_RS14320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_14315"
                     /product="hypothetical protein"
                     /protein_id="WP_083040398.1"
                     /transl_table=11
                     /translation="MNLVFFPKGYFLKNKSVKLLMGITFLLLFISTSFLTFSILDILSD
                     ETLSIEKQIATFVLIFFLAIPLYLILNFLSTVLTSIFMYFFDRHFVFRKMYFVILTYNA
                     FILLVNSIVLFCIMKLSLGHYLIIIQLLSFSVSTYFLRLLYHGIVHYAEGSEKGALAVS
                     LLYFVVTGIFTIGGILNG"
     gene            8224..8895
                     /locus_tag="B7C51_RS14325"
                     /old_locus_tag="B7C51_14320"
     CDS             8224..8895
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017358054.1"
                     /locus_tag="B7C51_RS14325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_14320"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_083040400.1"
                     /transl_table=11
                     /translation="MVRLQVSQLSFQYEQNVETELLLRDINFDLKLGDRVCILGKNGSG
                     KSTLLKLISGLIPSKSNTILLNDHIRPRTNHFKRMVAYIPDKPLVYDALTGLEHKELIR
                     SLWGIQKEGKALYEEKFQDLCCFFDLTHALEMPVKHYSLGMKYKLFLNCMLARSPELVI
                     LDEPFTSLDETSQKQSISMLISAFSEKCILFTSHQRYLYTELGNRFFQLDDGNLKEIEH
                     L"
     gene            8892..9062
                     /locus_tag="B7C51_RS26700"
     CDS             8892..9062
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003214630.1"
                     /locus_tag="B7C51_RS26700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_024093118.1"
                     /transl_table=11
                     /translation="MKTYSIVVIAISLIAIMVVLVLNLIGVFIGKIILAAAAAFLVTSI
                     IRLIGLSKRRH"
     gene            complement(9082..9417)
                     /locus_tag="B7C51_RS26705"
                     /old_locus_tag="B7C51_14325"
     CDS             complement(9082..9417)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /locus_tag="B7C51_RS26705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_14325"
                     /product="transposase"
                     /protein_id="WP_257789049.1"
                     /transl_table=11
                     /translation="MTAGNSHDCVKGYEMLQAMDLTGKTVIADRGYDMNNILELIEKQH
                     ALAVIPSRKHRNIQRNCGWWLYKERHLVECLFNKLKHYRRLATRYDKLSCTFAAFLSLA
                     SILLWLN"
     gene            complement(9471..9845)
                     /locus_tag="B7C51_RS26710"
                     /old_locus_tag="B7C51_14330"
     CDS             complement(9471..9845)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /locus_tag="B7C51_RS26710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_14330"
                     /product="IS5 family transposase"
                     /protein_id="WP_077996683.1"
                     /transl_table=11
                     /translation="MEKRYEIRDDQWEKIKDLLPPERKPQGGRIAKDNRMMLNAMLWVA
                     RSGAPWRDLPEHYGSWKTVYTRFRRWQMAGIWDEILKQVSVSPDLENIMIDATIVRVHQ
                     HGAGAKGGSNFRTSDTPEAE"
ORIGIN
        1 gggaaggaag actccggcct atcactatga aacagttaga tacgttaaca gaaaccctcg
       61 acttaccaga aggacattac tatgatctat accttgctga gtgtttcttt aacaacagat
      121 tggcagttcc cagaatgaaa tcctttttaa ttcgctgttc cgagctgggg aaaaccgact
      181 tggtcattat ttagacaagg ccattatgag aagacgaaac aatatataga aggatatgaa
      241 gaccttagtt ggtttgagat tttagacgag caaggaaaga aagaagtaaa taacttttct
      301 ctttgggcaa gagcgaataa atacagtgta gaactaatgt tagggaatgt cagtgtactt
      361 gatgaatacg caaattatct tgccgaacgt cctaatgata ttcctgaagg tcttcttatg
      421 attacacaag ccgcaaatgc acacgggttt tccatagatc atatcttaga acagttccct
      481 gagccttctt tagaaaatga tgtaaacgtt gtaaggattg aatatcatat tgaattttat
      541 taccaaaaag gaatatatga gttgaatcaa cagcgcttta ctacaggatt ggaatccatc
      601 ttacactgtc tatccctgtc tatccctaca aaaagacact ctatatctat attgtgtgca
      661 gcccaatttg agcagtatca aaacaatgct tcggaccccc aaagggaaaa attcggaaac
      721 ttgatgaaag aggtgttgga ggttgaaaaa atctaagaag ttgctattaa gtgttgtatt
      781 ggctaccgca atactaggca gtggaattac tctcagtacg ttacaagtta cttctgaagg
      841 ttctgctgtc gctgctatac atggatctgg tcagtcacct actatgtctg ttgctatcat
      901 acatggatct ggacaataaa aaatttctag gccctaaata accggcttct tgagccggtt
      961 attttcacta cctcttaatc cggatcggga aatccagagt acacttacag gctttcacgc
     1021 gcatgcctct tcgtaagaaa aaagagctaa agtattttat ttacccccgt aaagtacaaa
     1081 attaaaactg atatacccta atcaacctta gtccggtatt ccactggact aaggttgttt
     1141 aattttgttt gaaacctgtg catataacta gtagaagaga tagagtttgt atttacagaa
     1201 gcaattaccc aaacagatga acagaaatat gtactgaatg aagataagtt ggtagagaag
     1261 ggactccaac aagaagaaat caatggaatc aaacaatttg gtggcttact taaataataa
     1321 agaagtatct aatttcaata atgctcctga actgaggaca tctttcataa tttgtctgaa
     1381 agataaaata atgcaagaat ttgaggatct cgttgggatt ccgtatattc ctggaagttc
     1441 tttgaaggga gtggtccgaa actgctggct gcatgagcat ttggatgggg atttaggatt
     1501 aggggagaat gctactcgga aggttttttt tgacaagtgc ttcggaacag gcgctgatga
     1561 tgaacagttg tcttcaggaa agttaatctt tctggatgcg taccctgttg agacgtatca
     1621 aatcgtaaag gatgtgcaga ctccgcatta taaaacctac tataatcaaa agggcagtag
     1681 ccctccacgg gatgatgaca gccccaatat tatcccactt atggcaattg aagataccat
     1741 atttgacatt tatattggca tgaattttaa aactgatgaa ttagatgcta atctgcgtaa
     1801 tgaaataagc aaaacgatta ttagtacgct cagtgagtat ggagtgggtg ctaaaacagc
     1861 cattggttat gggttaggtg aagtaacgat gtgtgatagc tgaattggtt tacttagtct
     1921 ggactgattc tccggtagat catctgcatt cagtaaagtg acatgatagt gcatctctcc
     1981 ctttgaggta aacttaccat atatgggctt aggggcgcag cccctaacaa gcgaatttgg
     2041 atatccaaat tcagtgcttg caacaatacg agacagcatc tcgtgttcag actgtcgaca
     2101 aagtcctgtc gacagtcttt tttacgaaaa tactccaacc attcatcgca ttcatatagt
     2161 atcatatagg taaataacta gtgcgggagt gcttgagtat gttgcgaaaa accggttacg
     2221 aaggacgttc tcaaatttcc atggtctcct tggacgagct tttacctaaa gatcatttgg
     2281 tgcgtaaaat cgaatacgcc atcgatttta gtttcatcta tgacttggtt cgggatgttt
     2341 actctgaaga taacgggctt taaatcgggg cggtgacctt cttcaaatcc ttgtaagaga
     2401 cataacgggt agaattccgg atctggtgga taatgcattt ttggatttcc gtcttgggat
     2461 agctcgccgt aatggcctcg gagaacccat tcaggttgtc cacgcacgta ataagaatgt
     2521 cctggacgcc acgatttttc aggtcattca gcacgctgag ccagaacttg gatgactcgt
     2581 tttcgccaat ccacatccca agcacgtctt tgttgccatc caaatcgatg ccaatgacca
     2641 tgtacgccgc cttgctgacg atggcccctt cctgtttgac cttgaagtgg atagcgtcga
     2701 gaaaaacaac cgcgtatacg ccttgcaagg ggcgattctg ccattctttg atgaggggta
     2761 caatcttatt cgtcacattg gaaatgagtg tgggcgatac ctcaatacca tacagatttt
     2821 gtaggtgatc ctgaatctca cggggtgctc acgcccttag cgtacagagc tacaatttga
     2881 tcctcaatac cggtaacgtt ggattggtgc tttttgacca ccagcggctc aaactcaccc
     2941 atccggtcac ggggaactgg gatctgctgc tctccatatt cgctgactac cgttttatgg
     3001 cttcttccat ttcggctatt tggcgtcttc ttggccttga cctcatgctt ttcgtacccc
     3061 aggtgcgtgt ccagttccgc ctccagcatc tcctgagagg aatattttga tctgtgcgac
     3121 agcgtggata gtatgcatgt tgaagcagct ttcctgcatg gtttgcggga aaaccgcacg
     3181 agccgttcat tgaggggccg acctcgaaag aggttggtct actctatcaa aagtctatat
     3241 tccaaggcat actcaataag ttcacttttt ttagaaatat taagtttatt catcattctt
     3301 gtcttatggg tatcaaccgt tttgatgctg accgttaatg ccttggccgt ctcagcaaga
     3361 gaatatcctc tgacaagaaa tcgcaatact tcgcgttcgc ggggacttaa aatgacgtaa
     3421 ggattccgcg aatctgcttc ttttggtact tttttaagca tcgatgaaat caatgtcttg
     3481 gtttcttttg gccgaagata catatatccg ttcataacgg aatctatagc cgtgtataat
     3541 tcttcatcta ctgctgcctt tgggatataa cccgaagctc ctgcttgcat gatagatgta
     3601 atgtaatcct catcctcatg catggtaagc acaatgactt tggcttcttc atgtcgcaat
     3661 ttaatttctc tgagaacctg aatgccgtct aagcgcggca agctgatgtc gagtattaaa
     3721 agatggggtc ttaaatcttc gtaaagccgg agtgcttcaa ttccattagc ggcttcacct
     3781 acaactttga agggtggttt cttttgcagc agcagtttta accccgatct tagcaaagca
     3841 tggtcatcaa ctagcatgat ttgaatggtc tgttccatca ctgcaccctt tttccagttt
     3901 tagcgggata gatactataa gttccgttcc gccgatattt gtagttctga cgaagaaaga
     3961 accgccgagc agctcagctc tttccttcat accgtatagg ccgatccggt tttgccggcg
     4021 ggccctttca aaatcttcct gttggatacc atgaccgtta tcatgaacag tcaattgtat
     4081 acaatgatct gcaagcctca atccaatcac aatccgtgtt gccgccgtat gtttgactac
     4141 attagtgagg ctttctcgaa gaatccggta caaggctaca gcagtacgac tatcgatggc
     4201 gatatcctct tcaggggcgg ataggacaac cgcaatgcca tacttctctt caaacttctg
     4261 gatatatttc tttattgccg gaattaatcc catatcatcc aaaatagggg gccttaattc
     4321 taccgccaaa tctctaattt cccgcaaaat gcttgcagca atctcccggc tggtattaag
     4381 cagttcctgc tgttcagcgt ctttggcttc attagccaga acacgcatgg taaccatcaa
     4441 tgaagttagg gcttggcttg tttcatcgtg aagctcccgg gaaattctct ttctctcatc
     4501 ttcctgggca gacaataact ttaagatcag cgtatcccga agccgttctt tttcttgaag
     4561 ttccttcaac agcttttcta tttccgtatt agagctaatc agattgtccg ccatttcatt
     4621 aaaagcctgt gccagttttc ctacctcatc atcacttgtt gctttagccc gaagggacaa
     4681 atttccggcc gatatgcctg ttgcaacagc aaccaggtta tttattggct tgtgtcactt
     4741 cgtttaatgc gaattgatta ttcatcttaa tccctgcctg tctgaatggg tttccttatc
     4801 ttattcgaca agcaggtgta catttcctta ccattctttt ctaaaactta aattcaactt
     4861 atatagtttg cttctatcca tcacccctta caaagaatgt tgttaaatag aggaatttat
     4921 ataaaaatat gtattgataa tgattcaaac gaatgataga ctataaatgt cataaaatcc
     4981 attataagga ggaatcattt gtggtagcaa caagaaatga aatcaaactg tctcttcttc
     5041 tcggtgcttt agcaatggga tttttgatgc tggctctctt cttgttctct ctccaatttc
     5101 tcccagtcgc agatttagca aaagaattcg gtatcccagc tagtatcgct gcagttgtac
     5161 taaatattgt gaatgcagga ggtgctgtca caacaatcgt tagtattttg ttagccgttg
     5221 gttcaggcgg tctttcttta cttgctgctg caggtaaaga aacaattcgc cagttcttga
     5281 aaaagaaaat tcaagaaaaa ggaaaaagag ctactattgc ttggtaattc tcaactttta
     5341 ttaaatagaa ggggaaactc cccttctatt tatcatttta ctttgtaggt gagagtatgc
     5401 tattttcact gttatctcta cttctatcgg ttagaacgaa acagcaatat tcgaacatca
     5461 ttcaatggat cattggaaag gtttcttttt tgaagcgatt gtcgtcaaaa cagtatataa
     5521 tttttttatt ttttcttgat attctctttg gtcttaccgt tgtatctgtg atacatgctt
     5581 ggctttattc ccctgcctac cgacaacaat gggaaagtgg tttcattgtt ttcttactac
     5641 tttattccat tttaaaagga gttaattctt atcgaaaaac gcatattccg cctttccaag
     5701 aattgataga gcttgcaccg gttccggcaa aaaatattca tattacatca gttatcgcag
     5761 aatggatctg gattggattt acgaacttca gtactttttt catttatttc atgtttcaag
     5821 gatatattac agattcaatt gatttctact tttggatcaa acatgtcaat gtattaatat
     5881 tgagtatctt cctttttgta ctcagtaata agttatatgg tgcttatatg tacaacattg
     5941 ttgtgaaaaa aatcggttgg attcggctca tttttttctc tgctgtgtcc tccatatttt
     6001 tcatgttggg acgccttttt atttcaggtt ttattcttcc cttttatgaa gcctttagac
     6061 agaattttca ctcgatccat tcggctgttg ataatgcgtc ctggattcaa ttatctgagc
     6121 agttagtttc tctttattct aatcagctcc attccattca ggtatttttg tttttagaag
     6181 ggtccacttt tcttcaagtt cagcagttta taagtttgcc tgctgcaaca ctgctgctcg
     6241 cggcaggtgt attaggattg cttatcatac ctgccaaatg gataccgact gaggagacga
     6301 acagcacgag ccattttaag gatttatttc attggtactt acgtgccctt cacaaggtgc
     6361 gttacttgat atttcgagct gatctgctca tttacaagga cttgagggtg ttgcaaggga
     6421 aaaggtggct tctgtcaaag gaatttttca attactcttt catttcgtat gaagctgttt
     6481 tttatgcggg ggtctttaca ggatttctgt cagctgtgga gagcccggcg cttcaggttc
     6541 agttactttt cacaatgaat gtcatcatta tggcaaacca agcattagaa atgagagtat
     6601 tgacgccttc attattctca attagcgcgg aaaaacagaa tatttggctg tatcagcttt
     6661 ctctctcttc actttttcag ttcttcaggt caaaaatcaa gctgttttac tgcttatttt
     6721 tttttccatc cctgtttctg acgctgttta atttctttat gatgcttatc tatgagatct
     6781 atgatgtcca aatggtgatt cttattatcg gtatatttat tggttttttc atatttccat
     6841 tgattcagct ctatttgttc ccatttgtta cgaaattcaa ctttatacat gagtatgaaa
     6901 tcgggacgac aaaagaagaa acaggtattg tggataaagg acaatcattt atacgaagtt
     6961 ttcttgttat gccatttatt tactacttaa ttgtatcctc tttttttccg gttttacagc
     7021 attcggttaa agtagtgaat gttctttcca tcctatactt ttctggagtt tcatttattt
     7081 gctggattta ttgccataaa atgatatcaa agggaatagg gtatgttgaa aaaattaaaa
     7141 tctattctat cagcgaataa acttgtgtgt gtacttggat tcttctactt aggagtgttt
     7201 tttttagcag tttgtaccgg gagttttaca caggcagagc gtattcccca tttagaagat
     7261 aacaatcaag catcctttct tgatattttt ctaaataaca ttcaagtgag tatggccata
     7321 ttggggattg gttctataac tggcgggctg atgtcagctg ttatcctctt ttataacggt
     7381 tatattatag ggaaattggt gcaatttctc ttttcgaatg atcaagcagg tgcgctttgg
     7441 acgggtttaa tgccgcatac cctattagag attctggggc ttactctttt tgcaattgtc
     7501 agctcttttc cattcatttc tatttatcaa tttttgatag gcagaactat tgatgtcaaa
     7561 cagcttatac tgacgaacat tcgtttcatt ctcataggca ttcttctctt atttgttgct
     7621 tctcttattg aagattatat cagttatgta caatttatcc tcgaattgaa aggagaaaga
     7681 tgaatatgaa tttagttttt tttcctaaag ggtattttct taaaaacaaa tcagtaaaat
     7741 tactaatggg tattaccttt ctattgttgt ttatatcgac ttcctttttg acattttcta
     7801 ttttagacat tttatcagat gaaacgttat ctattgaaaa gcagattgcg acttttgtct
     7861 taatcttttt tttggccatt ccgttatatc ttatattgaa ttttttgagt acggttttga
     7921 cttcaatttt tatgtatttt tttgatagac attttgtttt tcgtaaaatg tactttgtca
     7981 tccttactta taatgctttt atcctgttag tgaatagtat tgttctattt tgtatcatga
     8041 agttaagttt aggacactat ttaattataa tccaattgct ttctttttca gttagtacat
     8101 attttttgcg cttgctttat catggaattg ttcattatgc ggaaggatct gaaaaggggg
     8161 ctttagcggt ttctctttta tattttgtag taactggtat atttaccatt ggaggaatct
     8221 taaatggtta gactgcaagt ctctcagtta tcatttcaat atgaacaaaa cgttgaaact
     8281 gaattactgc tcagggacat taactttgat ttaaagctag gtgatcgggt atgtattctc
     8341 gggaaaaatg gaagtggtaa atcgacacta ttgaagctca tttccgggct gattccatca
     8401 aaatcaaata ccattttatt aaatgatcat atccgtccaa gaacgaacca cttcaagaga
     8461 atggttgcat atatccctga caaacctctt gtatacgatg cactgacagg tcttgagcat
     8521 aaggaattaa ttcggtctct gtggggaatt cagaaagagg gaaaagcatt atatgaagaa
     8581 aaatttcaag atctttgctg cttttttgac ttgacacatg cacttgaaat gcctgtgaaa
     8641 cattactctc taggtatgaa gtataaactg tttttaaatt gcatgctcgc gaggtcgcca
     8701 gagttggtca ttcttgatga gccatttact tcccttgatg aaacttcaca aaaacaatcg
     8761 atttccatgc tgataagtgc cttttccgag aaatgtatat tgtttaccag tcatcaacgc
     8821 tatctgtata ctgaactggg aaaccgtttt tttcagcttg acgatgggaa tctaaaggag
     8881 attgagcatt tatgaaaact tattcaatcg tcgtaattgc cattagtctg attgccatta
     8941 tggttgttct agttttgaat ttaattgggg tttttattgg taaaattatc cttgctgccg
     9001 ccgctgcctt cttggttact tcaataatcc gattgatcgg tctatcaaaa agaagacatt
     9061 aactagagtg cgtcttcaaa cctaatttaa ccataacaaa atagaggcca atgatagaaa
     9121 ggccgcaaaa gtacaggata gcttatcata gcgagtagcc agcctacgat aatgtttaag
     9181 cttgttgaat aaacattcca ccaagtggcg ttctttatag agccaccagc cgcaatttct
     9241 ctggatgttg cgatgtttcc gacttggaat gacagcaaga gcgtgctgct tctcaatcag
     9301 ttccaaaata ttgttcatgt cgtaacctcg atcggcaatc acagttttac cagtgagatc
     9361 catagcttga agcatttcat agcccttgac acagtcgtgg ctattgccag cagtcagttc
     9421 aaatcataac ggatttccaa gtgcatctac aatggcatta attttggttg ttattccgcc
     9481 tctggagtgt ccgacgtcct gaaattgctg cccccttttg cgcccgcccc atgctgatgg
     9541 acgcggacga tggttgcgtc gatcatgata ttttccaaat cgggagatac ggaaacttgc
     9601 tttagaattt catcccatat gccggccatc tgccagcgtc gaaaacgagt atacactgtt
     9661 ttccatgaac cataatgctc gggtaaatcc cgccaaggtg ctccagacct tgcaacccaa
     9721 agcatggcat ttagcatcat gcgattgtct ttcgcaatgc gcccaccttg aggttttcgt
     9781 tctggtggca gtaaatcttt aattttttcc cattgatcat cgcgtatttc gtatcgtttt
     9841 tccatatgga aagtttatca tgttttggag tgaacttata gtttgaagac acactctaga
     9901 tagctatgca ggaaatcaag tacctgggca aagtcctgcc gatgttttcc ttgtctatct
     9961 gcctgtcgaa taagtagtcc tcttccggaa gggttaggaa agtgtgcttt acccccgtaa
    10021 attttaatct ttccgccatg aaaacggatt aggcgggtac ccgcatcaat ccaagaaaac
    10081 ctggttcgct gttttttcgt tatcatatgt gttctggtaa agctagggtt accatttgaa
    10141 gtgaaagcaa attactgttg ttccaatgga ttcatagcaa attacagaat caaatttata
    10201 aataaaatac tttaaaggaa ttaattagat tattaagcag ttgttgactg gaaaaataag
    10261 ttgatgatac attagatgta tgaaattaag ggaaaaatag gttggcaccg tctttggaac
    10321 tactacg
//
