LOCUS       NZ_CP020557            10291 bp    DNA     circular CON 24-JUL-2024
DEFINITION  Paenibacillus larvae subsp. pulvifaciens strain SAG 10367
            chromosome, complete genome.
ACCESSION   NZ_CP020557
VERSION     NZ_CP020557.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus larvae subsp. pulvifaciens
  ORGANISM  Paenibacillus larvae subsp. pulvifaciens
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 4666454)
  AUTHORS   Dingman,D.W.
  TITLE     Paenibacillus larvae genome sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4666454)
  AUTHORS   Dingman,D.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2017) Entomology, CT Agric Expt Station, 123
            Huntington St, New Haven, CT 06504, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2016
            Assembly Method :: SMRT Pipe HGAP assembly v. February-2016
            Assembly Name                     :: sag10367-hgap-022916
            Genome Coverage                   :: 214.2x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002082155.1-RS_2024_07_24
            Annotation Date                   :: 07/24/2024 02:27:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,275
            CDSs (total)                      :: 5,166
            Genes (coding)                    :: 4,634
            CDSs (with protein)               :: 4,634
            Genes (RNA)                       :: 109
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 532
            CDSs (without protein)            :: 532
            Pseudo Genes (ambiguous residues) :: 0 of 532
            Pseudo Genes (frameshifted)       :: 400 of 532
            Pseudo Genes (incomplete)         :: 184 of 532
            Pseudo Genes (internal stop)      :: 26 of 532
            Pseudo Genes (multiple problems)  :: 72 of 532
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:07:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2528194
            Orig. end                         :: 2538485
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020557.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Dr. Adriana Alippi,
            CIDEFI-CIC, Facultad de Ciencias Agrarias y Forestales, Universidad
            Nacional de La Plata, 1900 La Plata, Argentina.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10291
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="11"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10291
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10291
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5291](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5291)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(298..312)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 20.22"
                     /tool="antismash"
     misc_feature    651..666
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 21.07"
                     /tool="antismash"
     gene            706..975
                     /locus_tag="B7C51_RS13455"
                     /old_locus_tag="B7C51_13455"
     CDS             706..975
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019914358.1"
                     /locus_tag="B7C51_RS13455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_13455"
                     /product="HPr family phosphocarrier protein"
                     /protein_id="WP_077996534.1"
                     /transl_table=11
                     /translation="MSRRPVVVKLKTGLHARPAALFVQEANKFSSEIFVEKDEKKVNAK
                     SIMGIMSLAISTGTEIYISAEGSDDEQAVNELVSLVSKEELENQ"
     gene            complement(1085..1318)
                     /locus_tag="B7C51_RS13460"
                     /old_locus_tag="B7C51_13460"
     CDS             complement(1085..1318)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B7C51_RS13460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B7C51_13460"
                     /product="hypothetical protein"
                     /protein_id="WP_077996535.1"
                     /transl_table=11
                     /translation="MKIIHIGTTIGLLLYFKFFIINGVFNPSMDMMIGLVSLSIFYLLV
                     EYILKRYGYEHKKVWMGWAILILLITSLIFLI"
     gene            complement(1345..1968)
                     /locus_tag="B7C51_RS13465"
                     /old_locus_tag="B7C51_13465"
     CDS             complement(1345..1968)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /locus_tag="B7C51_RS13465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_13465"
                     /product="YIP1 family protein"
                     /protein_id="WP_083040276.1"
                     /transl_table=11
                     /translation="MKIRSLFQIFFHPKQVLQTNLQHGNLFFPILFISLLSGAFSLSTV
                     WALPDNFMGESYLKTIASVFAIFYACLTPLFSILILSIINYIALAALDAFTPFRKLMII
                     GLYAYMPLFIDSALKLVMTLYLGKAIAFSPTSLTLLLPPDAPFLLNRFINSLSFSGLWS
                     IGIYLLGVSLSVPKTQKRKVVTILLVLNILLSIIPNFLIMGPMK"
     gene            complement(2002..2595)
                     /locus_tag="B7C51_RS13470"
                     /old_locus_tag="B7C51_13470"
     CDS             complement(2002..2595)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /locus_tag="B7C51_RS13470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_13470"
                     /product="stage II sporulation protein M"
                     /protein_id="WP_077996537.1"
                     /transl_table=11
                     /translation="MMNMKQVSEHQAKWDQINKRFKSEKWIQKNVVLGLFIASGCIFFL
                     SFLLGALFSRNFSVNIDHTLTLSRDPFYYIIHNLQSSLYMIGGLFSFSFTTLWALFING
                     YYLGVTFTGIGELYSFSTAAGSIAAHGVFEIPAILLASATGLYPWYFIYCFLKNKKIRY
                     KEHLKNSISMLVLSVVLFILAGIIEAKISPLFVQ"
     gene            complement(2573..3292)
                     /locus_tag="B7C51_RS13475"
                     /old_locus_tag="B7C51_13475"
     CDS             complement(2573..3292)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="B7C51_RS13475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_13475"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_023485031.1"
                     /transl_table=11
                     /translation="MICVEKASFQYENSVSKAVNSVSFSLREGEVLGLLGHNGAGKSTL
                     LECLSGLRKLTGGNMTADHLPVDQYKKTSFIPNDLYLYNLLTVEETFLYIGRLHSLSYD
                     QIWKIADPLIQMFSLQEKRKEYVKHLSFGMKQKVALILGILDSPRYLLLDEPMTGYDPL
                     STKTTKEFLLHLAKEQKTGILLSSHRLDIVEDLCDRVLVIHQGVLVFDGSISSLKHDRS
                     FEEALLSLVQDDEHEAS"
     misc_feature    3255..3269
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 22.62"
                     /tool="antismash"
     gene            complement(3591..4943)
                     /locus_tag="B7C51_RS13480"
                     /old_locus_tag="B7C51_13480"
     CDS             complement(3591..4943)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B7C51_RS13480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B7C51_13480"
                     /product="hypothetical protein"
                     /protein_id="WP_083040278.1"
                     /transl_table=11
                     /translation="MKTIKHIGLLIGLQHLLHFRWLVSRILPNTVISLKSLLQIVAGAW
                     VLVGLFTYFSCYALFLVLQNNRITVSLDLAAKMEFLFCLLFFSYLLTHSFQEVYIRAFS
                     SQDYFLLRTRGQSPFAVQFAKLVDAFFLEVLLYGIPFQAGIIIAIIQAAGSYSPILWIL
                     LFWAIVLFALLCKMSLMVLLFAVNKPSFRIGSFSTRVIFFLFQLFIGAGISYAIIRYLN
                     NRQFFSHWMQAFMEWKWDNGFLITGYVWVVLASLLLMPAVIAPVYVCNKSWDISRWNKE
                     EQGKETNKRSFLTRFFMSPGIRSKTLNLIMKDLLLTTRGETISWEFIKKVWFSSSCMLG
                     GFAAFSYHLTGKEQLSQGVLLLLVIISQMILAALATIAAGKVTSLDTERNWIILHFVRL
                     ENPYFLYRAKAILHFFFVFPIVCIHTFIILLFIPVSPWVRYMYWQARFVSA"
     gene            complement(5001..5291)
                     /locus_tag="B7C51_RS13485"
                     /old_locus_tag="B7C51_13485"
     CDS             complement(5001..5291)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03651.1"
                     /locus_tag="B7C51_RS13485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_13485"
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="WP_077996539.1"
                     /sec_met_domain="TIGR03651 (E-value: 4.8e-08, bitscore:
                     31.4, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDIHIFALLVMIEYEIKRGEIKLDLFNSIVEIAGTFKISMDTSTK
                     VYDAVMAGADMWAVISIIAAVTGPLAIGGAVVFYMIKRKIKGMARSAAIAW"
     gene            complement(5447..6028)
                     /gene="clpP"
                     /locus_tag="B7C51_RS13490"
                     /old_locus_tag="B7C51_13490"
     CDS             complement(5447..6028)
                     /EC_number="3.4.21.92"
                     /GO_component="GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA]"
                     /GO_function="GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene="clpP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533314.1"
                     /locus_tag="B7C51_RS13490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_13490"
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="WP_023485033.1"
                     /transl_table=11
                     /translation="MSFIPMVVEPDARGERSYDIYSRLLKDRIVFLGTQVNDAVANSII
                     AQLLFLAAQDPDKDISLYINSPGGSITAGMAIYDTMQYIKPDVSTICVGMAASMGAFLL
                     NAGAVGKRYALPNSEVMIHQPLGGAEGQATDIEIRAKRIIKMRENLNRILSERTGQPLE
                     KIERDTDRDYFMSASEAKEYGLIDKVIERV"
     misc_feature    complement(6085..6099)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.58"
                     /tool="antismash"
     tRNA            complement(6217..6288)
                     /anticodon=(pos:complement(2534447..2534449),aa:Arg,
                     seq:ccg)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="B7C51_RS13495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="B7C51_13495"
                     /product="tRNA-Arg"
     gene            complement(6217..6288)
                     /locus_tag="B7C51_RS13495"
                     /old_locus_tag="B7C51_13495"
     gene            6564..7590
                     /locus_tag="B7C51_RS13500"
                     /old_locus_tag="B7C51_13500"
                     /pseudo=""
     CDS             6564..7590
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676835.1"
                     /locus_tag="B7C51_RS13500"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_13500"
                     /product="sugar-binding transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRKILDIQKQLVPDMLRILNKRYTILHQIRISGTIGRRTLAGSLD
                     MTERVLRGEVDFMKAQGLIEIETSGMRISEEGTKVLDELEPMMKDILGLTHMEQQICEK
                     FRIKQAVIVPGDSDTSSFTRKELGRAGASVLRKYLSDNDIIAVTGGSTMAEVANHLLAS
                     GNMKNSCFVPARGGLGESMEFQANTIASNMAKKTGAQYRLLHVPDHLNEEAYQSLMKDP
                     NIQERMQVIRESRIVVHGIGDAMVMARRRKVDQETIKNLQKDGALAEAFGYYFNKDGEV
                     VHKMSTVGLRLEDIQNTEIVIGIAGGKARGSNCFRA"
     gene            7647..8651
                     /gene="gap"
                     /locus_tag="B7C51_RS13505"
                     /old_locus_tag="B7C51_13505"
     CDS             7647..8651
                     /GO_function="GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_function="GO:0051287 - NAD binding [Evidence IEA]"
                     /GO_process="GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="gap"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010500417.1"
                     /locus_tag="B7C51_RS13505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_13505"
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="WP_083040280.1"
                     /transl_table=11
                     /translation="MVKVGINGFGRIGRNVFRAALNNKDVEIVAINDLTNVATLAHLLK
                     YDTTHGVLDATVEAGEGEIKVNGKSIKVYAERDPQNLPWGSLGVKIVVESTGIFTAKEK
                     AEAHIKAGAKKVIISAPAKNEDITIVMGVNEDKYDAANHHVISNASCTTNCLAPFAKVL
                     DEKFGIVKGMMTTVHSYTNDQQVLDLPHKDLRRARAAAENIIPSTTGAAKAVALVLPQL
                     KGKLNGMAMRVPTPNVSVTDLVVETSKNVTVEEVNAALKEAANGPLKGIMNYSEEPLVS
                     KDYNGDPASSTIDSLSTMVVGDNLLKVVSWYDNEWGYSNRVVDLVNYIASKGL"
     gene            8789..9970
                     /gene="pgk"
                     /locus_tag="B7C51_RS13510"
                     /old_locus_tag="B7C51_13510"
     CDS             8789..9970
                     /EC_number="2.7.2.3"
                     /GO_function="GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="pgk"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016820125.1"
                     /locus_tag="B7C51_RS13510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_13510"
                     /product="phosphoglycerate kinase"
                     /protein_id="WP_077996541.1"
                     /transl_table=11
                     /translation="MNKKSVRDVEVAGKRVFVRVDFNVPLENGQITDDTRIRETLPTVK
                     YLIEQGARVILASHLGRPKGQVVEEMRLTPVAKRLSELLGKPVVKADVAIGAEAKEKAE
                     QLKNGEVLLLENVRFYPGEEKNDPELAKAFAELAELYVNDAFGAAHRAHASTEGIAHHL
                     PAVSGLLMEKELDVLGKALTNPERPFTAIIGGAKVKDKIDVINNLLNLADNIIIGGGLA
                     YTFLKAQGNEVGKSLVDNEKIDLALGFINKAKEKGVNFLLPKDIIVADDFSNTANTKVV
                     AVNEIPADWEGLDIGPETCKLYAETIKNSKLVVWNGPMGVFEMDLFAEGTKAVAQACGE
                     TEAYTIIGGGDSAAAVEKFKMADKMDHISTGGGASLEFMEGKALPGVVALQDK"
ORIGIN
        1 gttttgacct taatgccagg ttcattgaac gtaaaaaggg ttttgtgttt tacatcaaag
       61 aaggtgagaa aatcatcgaa ttcctcagca tcattggggc tcaccaggcc ttgttcaagt
      121 ttgaagacgt acgcatcatg aaagatatga ggaattccgt aaaccgcatt gtgaactgtg
      181 aaacggcgaa cctcaacaaa acgatcggcg ccgctgtcag gcaaatcgat aatatccggc
      241 tgcttcaaag ggagatcggg ttagaaaacc ttcctgaaaa attaagagag gttgccgagg
      301 tccgtttggc ccatccggat atgaacctca aggaagttgg cgatttgcta aaaggcagtg
      361 tcagcaaatc aggagtgaac caccgtcttc gcaaaattga tgaattagct gagaagatta
      421 gacaaggcaa atagaatgga gctagtttat ttctcggcaa aatggtatac tatttaatga
      481 tgcctttttt tcgaaaaggg caacacgcat aagttttcct taataacttg aatccatttc
      541 atatgaaatt gattctttgt cttatcttca ccggaaaact ccaccgtctt caagacggcg
      601 aagccgtttt ttcttggtta tgtacggtcg tcttatccaa gacgcttcta atacccattg
      661 gaagtaggca gcattgcttt taataaagaa gtaggggatt gattcatgtc gagacgtcca
      721 gtagttgtga agttgaagac aggacttcat gctagaccgg cagctttgtt cgttcaggaa
      781 gctaataagt tttcttccga aatttttgtg gaaaaagatg agaagaaagt caacgccaaa
      841 agcattatgg gaattatgag cctggctatt agcacgggga cggaaatcta cataagtgct
      901 gagggttccg atgatgaaca ggctgtgaat gagcttgttt ctctggtaag caaagaagaa
      961 ttggaaaatc aataagtgaa gacaaatggt taaaaaccag ccaattcctt taaggagtta
     1021 ggctggtttt gtcgtggaat gctgatgaat tcaacaagga atggattgtc cgatccatat
     1081 aggatcaaat aaggaagatc aggctagtta tcagaaggat aaggatggcc caacccatcc
     1141 atactttttt atgctcataa ccgtacctct tgagaatata ttcaaccagc agataaaaga
     1201 tagacaagga aaccagacca atcatcatat ccatagaagg attgaaaaca ccattgataa
     1261 taaaaaattt aaaatacagt agtaaaccaa tagtggtacc tatatgaata atcttcatgt
     1321 ccgctcaccc caccggcaat tattctactt catgggaccc attatcaaga aattcggtat
     1381 aatacttaac aaaatattta aaactaacag gatagtcacg acttttcttt tttgagtttt
     1441 gggtaccgac aaagacaccc cgagcaggta aatgcctatg ctccacagtc cgctaaagct
     1501 aagactgttt ataaatcggt taagaagaaa tggcgcatcc ggcggcaata acaaagtcaa
     1561 agatgtcgga gagaacgcaa tagcttttcc cagatagagg gtcatgacaa gttttaaagc
     1621 tgaatcgata aaaagcggca tataggcata taaacctata atcatcagtt ttctgaacgg
     1681 tgtgaaggca tccagggctg ccaaagcaat ataattgatg atgctaagaa ttaagatact
     1741 gaataatggg gtaagacagg cataaaaaat agcaaataca ctggctattg tcttgagata
     1801 gctctcaccc ataaagttat caggtaaggc ccaaacagtc gataaagaaa aagctcctga
     1861 taataggctg ataaacagga tggggaaaaa taaatttcca tgctgtaggt tcgtttgtaa
     1921 tacctgttta ggatgaaaaa agatttggaa caggctcctg atcttcattt tattttctcc
     1981 tctcaagcct cgatacatga ttcattgaac gaataaagga gagatttttg cctctataat
     2041 cccggctaaa ataaaaagaa cgactgaaag aactaacatc gaaatgctgt ttttcaggtg
     2101 ctctttatac cttatttttt tattttttaa aaaacagtat ataaaatacc aaggatataa
     2161 accggtagca ctggccaaga gaatggcggg tatttcaaaa acgccgtgtg ccgcgatact
     2221 gccagcagca gtactgaaag aataaagttc tccgatacct gtaaaagtca cccccagata
     2281 gtagccattg ataaatagtg cccataaagt tgtgaatgaa aaagaaaata gcccgcctat
     2341 catgtacagg gaactttgca aattgtgaat gatataataa aaaggatcac gggataaggt
     2401 tagagtatga tctatattca cagaaaaatt ccttgaaaaa agagcgccca aaagaaaaga
     2461 aagaaaaaag atgcaccctg acgcaataaa aagtcccaaa acgacatttt tttgaatcca
     2521 tttttcggat ttaaatcgtt tgttaatctg gtcccatttt gcttgatgtt cactaacttg
     2581 cttcatgttc atcatcctgt accaaactca acaatgcttc ctcgaaggaa cggtcatgtt
     2641 tcaaagatga aatggaacca tcaaacacca aaaccccttg atggatgacc agaacacggt
     2701 cacacaaatc ttccacaata tccaaacggt gagaggacag caaaatacct gtcttttgct
     2761 ctttagctaa atgaagtaaa aattccttgg ttgttttggt ggaaagaggg tcatatccgg
     2821 tcatcggttc atccagcagc agataacggg gggagtcaag tatacctaag ataagagcca
     2881 ctttctgctt cattccgaag gatagatgtt tcacatactc ttttcgtttc tcctgaaggg
     2941 aaaacatctg aataagcggg tctgctattt tccaaatttg atcgtaactt aatgaatgaa
     3001 gcctgcctat gtacaggaag gtttcttcaa ccgttaataa gttgtataga tacaggtcat
     3061 tcggaataaa acttgttttc ttgtactgat ctaccggaag gtgatctgct gtcatatttc
     3121 ccccggtcaa ctttcgcaaa ccgcttaaac attccaataa ggtgcttttg cccgctccat
     3181 tatgtcccaa taagccgagt acttctcctt cccttaatga aaaggatacg gaattcacgg
     3241 cttttgaaac gctattctca tactgaaaag atgctttttc gacacaaatc attttatttt
     3301 ccagcctttc catacaggtg ttcttaaaat aagaataagg agaacattcc aaacggccgt
     3361 agtcaaaagt aaacattgta aagaagaaat taaaaaatcc tgctcactta ttttctccgc
     3421 atataacaaa gcggccttca ccccgacaaa gctaatattg ataaaatcat aaaaaagaaa
     3481 cagcacactc tcggaaatag atccaagcag ggaagtatta atttggtctt tgttctccca
     3541 gccaaaatta ggaaaacaaa cgcttcccac tataaagctt aaagtcaagg ttaagctgat
     3601 acaaaacgcg cttgccagta catataccga acccaggggg aaaccggtat aaataatagg
     3661 atgatgaaag tatgaataca tacgatcggg aatacgaaaa aaaagtgcag aatagctttg
     3721 gcccgataaa gaaagtacgg attctctaat cggacaaaat gaagaataat ccagttcctt
     3781 tctgtgtcca aactggtcac ttttccggcg gcaattgtag ccaatgcagc taaaatcatc
     3841 tgagagatga tgaccaataa aagcaaaacc ccttgggaga gctgttcttt ccctgtcaga
     3901 tgatatgaaa aggcagcgaa gcctcctaac atgcaagagg aagagaacca tacctttttg
     3961 atgaattccc aagaaatcgt ttctcccctg gttgtcagaa gtaaatcctt catgatcaga
     4021 ttcagtgttt tagaacgaat gcctggcgac ataaagaacc gggttaaaaa ggaacgttta
     4081 ttggtttctt ttccttgttc ttctttattc catcttgaga tgtcccagga tttgttacat
     4141 acatatactg gagctataac agccggcatt agcaggagac tggcgagtac cacccatacg
     4201 tatccggtaa tcaagaaccc attgtcccat ttccattcca taaaggcttg catccagtgg
     4261 gaaaagaatt ggcggttatt tagatatctt atgatggcat aagaaattcc tgcaccgatg
     4321 aacagctgaa agagaaaaaa gatgaccctc gtagagaatg aacctatgcg gaaggatggc
     4381 ttgtttacag caaataaaag gaccattagg gacattttac aaagtaaagc aaacagtacg
     4441 atagcccaaa aaaggagaat ccataaaata ggggaataag aacccgctgc ttgaataata
     4501 gcaatgatga ttcccgcctg aaaaggaatt ccatataaca atacttccag aaagaaggca
     4561 tctaccagtt tggcaaattg aacagcaaag ggagactgtc ccctcgttct taagaggaag
     4621 taatcctggg aagaaaaagc ccgtatgtat acttcttgaa aagaatgtgt aagtaaataa
     4681 gagaaaaaca aaaggcaaaa gaggaattcc atcttggcag ccaaatccaa tgaaaccgta
     4741 atcctgttgt tttgaagaac aagaaataaa gcgtaacaag aaaagtaagt aaataatcct
     4801 accaaaaccc atgcacctgc gacaatttgc agcagacttt taaggcttat aacagtattc
     4861 ggcagaattc ggctaaccaa ccagcggaaa tgaagcaaat gctgtaatcc gattaataat
     4921 cctatatgtt tgatagtttt catcactaac tccttgggaa aaggcagaaa gtctgccata
     4981 gacttctgcc ttaaaacgat ttaccatgct atagctgcac ttctggccat ccctttgatt
     5041 tttctcttaa tcatgtaaaa aactacagca cctccaatag ctagcggccc cgtaacggca
     5101 gcaataatgg aaataactgc ccacatgtct gcaccagcca tcacagcatc ataaaccttt
     5161 gtagaagtgt ccatacttat tttaaatgta ccggctatct caacgatgga attaaacaaa
     5221 tccaatttga tttcacctct tttaatttca tattctatca ttactagtaa tgcaaaaata
     5281 tggatatcca agtagaatta tataattctt tctatttaaa tgtcaacaaa taacaaaaaa
     5341 accagtcgac ccctattaga ttcgactgat tttttgcttg gaataaacat ttcggagttg
     5401 aatcaatttc ataattgtga aattgattca gtttggaaca gattcattaa acccgctcga
     5461 tgactttatc aatcaatccg tactccttgg cttctgaagc actcatgaaa taatcacggt
     5521 ccgtgtcacg ctcgattttt tcaagaggct ggcctgtacg ttcggacagg atccggttta
     5581 ggttttcccg cattttaata atccgttttg cccggatttc aatgtccgta gcctggcctt
     5641 ccgcaccgcc gagaggctga tgaatcatca cttcactgtt aggaagggca taacgtttgc
     5701 cgacggcacc cgcattcagc aggaaagctc ccatggaagc ggccatccct acacaaatgg
     5761 tggaaacgtc gggtttaata tattgcatcg tatcataaat agccatacct gccgtaatag
     5821 agccgcccgg gctgttgatg tacaggctga tgtctttatc cggatcttgc gcagccagga
     5881 acagaagctg ggcaataatc gagttggcca ctgcatcatt cacttgagtg ccaagaaaaa
     5941 caatccgatc tttaagcaaa cgggagtaaa tgtcataaga acgttctcct cttgcatccg
     6001 gttctactac cattggaata aaactcataa cgagtatacc ctccttatcg ttccatcttt
     6061 cgtggatggt gtatgatttc aagtatctac aaatatgata acgaaaaaac gttttaaggt
     6121 caagaaaggt caaactgttt gttgaatgca gcaattaaat aaaaaactgt ccctgcgcca
     6181 ttcatcatga ctcagggaca gcttatgaag taaaaacgcg cccgcgaggg atcgaacctc
     6241 gatctcaggc tccggaggcc tgcgtcatat ccattggacc acgggcgcaa aataatattt
     6301 ccaacattct attttgatag attgaaactg aaacagcaaa attaattata cgtgtttggt
     6361 tacgaaaaat caagccccac aaaaaaggtt tatgggcacc attactttca atgaattttc
     6421 ataatcagga cggaaggcga gttgattctt taaagggggt cgtatataat gaagcgtgta
     6481 aaggaaattt ttttggacca tgtgggacta aaaatgcact cgtgggacat attaagtcca
     6541 ataattaaaa tcaggagtgt acgatgagaa agatccttga tattcaaaaa cagcttgtcc
     6601 ctgatatgct gcgaatcttg aataaaagat ataccatttt gcatcagatc aggatatccg
     6661 ggacgatagg ccgcagaaca ctggccggtt ctcttgatat gacagagcgt gtccttcggg
     6721 gtgaggttga ttttatgaag gctcaaggcc tgattgaaat tgaaacttcc ggcatgagaa
     6781 tcagcgaaga aggaaccaag gttttggacg aactggaacc gatgatgaaa gatattcttg
     6841 gccttaccca tatggagcag caaatctgcg agaaattccg gatcaagcag gcagtgattg
     6901 tcccgggaga ttccgatact tcctctttta cgagaaaaga gctcgggcgg gccggtgctt
     6961 ccgtactgcg aaagtattta tcggacaatg atattatcgc agttacaggc ggatctacaa
     7021 tggctgaggt agccaatcat ttattggctt ccggtaatat gaaaaacagc tgttttgttc
     7081 cggccagagg aggtctgggt gaaagtatgg aattccaggc gaataccata gcttccaaca
     7141 tggccaaaaa gaccggagcg caataccgtc tgcttcatgt tccggatcat ttaaatgaag
     7201 aagcctatca atctcttatg aaggacccga atattcagga aagaatgcaa gtgattcggg
     7261 aaagccgcat tgtagttcat ggaattggag atgctatggt tatggccaga cggcggaaag
     7321 tagaccagga aacaatcaaa aatttacaaa aagacggggc attagccgag gcttttggat
     7381 actactttaa taaagacggc gaagttgttc ataaaatgtc aacagttggt ttaagattag
     7441 aggatattca gaatactgaa atagtgatcg gtattgccgg gggaaaagca aggggaagca
     7501 attgcttccg tgcttaaatt cggccaccag gacgtactgg ttacagacga ggctgctgcc
     7561 atacagctta tgcatgatgt gttatcttga caggcctctg gcttgtcttg aaatatacaa
     7621 ttagacaata ctaggaggaa atgaacatgg taaaagttgg tattaacggt tttggtcgta
     7681 ttggacgcaa cgtattccgc gcagctctga acaataaaga tgttgaaatc gtggcgatca
     7741 acgacttaac taacgtagca accctggctc acttgctgaa atacgatact actcatggcg
     7801 tacttgatgc aactgttgaa gctggtgaag gtgaaattaa agtaaatggt aaatccatta
     7861 aagtatatgc tgaacgtgat cctcaaaacc tcccttgggg aagccttggt gtcaaaatcg
     7921 tagtggaatc cacaggaatc ttcactgcaa aagaaaaagc ggaagctcat atcaaggccg
     7981 gtgcgaagaa agttattatc tccgctcctg caaaaaacga agacatcacc atcgtaatgg
     8041 gcgttaatga agataaatac gatgcagcaa accatcacgt tatttccaac gcttcttgta
     8101 caacaaactg cttggcgcca ttcgcaaaag tacttgacga gaagttcggc atcgtaaaag
     8161 gtatgatgac aactgttcac tcctatacaa acgaccaaca agtgctggat ctgcctcaca
     8221 aggatcttcg ccgtgcccgt gcggcagctg aaaacatcat tccttccaca actggtgccg
     8281 caaaagcagt agctcttgtt ctgcctcaac tgaaagggaa actgaatggt atggctatgc
     8341 gcgttcctac tcctaacgta tccgtaactg atttggtagt agaaacaagc aaaaatgtaa
     8401 cggtggaaga agtaaacgca gcactgaaag aagctgccaa cggcccgctg aaaggaatta
     8461 tgaactactc tgaagagccg cttgtatcca aagactacaa tggtgaccct gcatcctcca
     8521 ctatcgattc cctgtccact atggtagtag gtgataactt attaaaagta gtttcctggt
     8581 acgataatga gtggggttac tccaaccgtg tagttgatct cgttaactac attgcaagca
     8641 aaggtctgta atttggatca gtcattcata aggacaacta agaggtgaag aggagaagca
     8701 attctcctct ttccccttga ttaacttgcc aaataaccag tggaaaggcc ggaaaggtat
     8761 ccacggaaag agcataggag gaaaacgaat gaacaagaaa agtgttcgcg atgtagaagt
     8821 tgcaggaaag cgtgtattcg ttcgcgtaga ttttaacgtt cccctcgaaa acggtcaaat
     8881 tactgatgat actcgaatca gggaaaccct tcctacagtg aaatacttaa ttgagcaagg
     8941 cgctagagtc atcctggcca gtcacttggg acgtcctaaa ggacaagtgg tggaagaaat
     9001 gagactgact ccagtagcta agcgtttgtc cgaactgctt ggtaaaccgg tggtgaaagc
     9061 ggatgttgcc attggtgcgg aagcaaaaga aaaagccgag cagttgaaaa acggcgaggt
     9121 tcttcttctt gagaatgttc gtttctaccc gggtgaagag aagaatgatc ctgaactggc
     9181 taaagctttc gcggagctgg ccgagttgta cgtgaacgac gcatttggtg ctgctcaccg
     9241 tgcccatgct tctacagaag gtatcgctca tcaccttcca gccgtatccg gtcttcttat
     9301 ggaaaaagag ctcgatgtgc tgggcaaagc tcttaccaat ccggaaagac ctttcacagc
     9361 catcattggc ggagcaaaag taaaagataa aattgatgtt attaataact tgctgaatct
     9421 ggcagacaac atcattatcg gtggaggcct ggcatataca ttcttgaaag ctcaaggaaa
     9481 tgaagtcggt aaatcccttg tagataatga aaaaattgat ttagcccttg gttttattaa
     9541 caaggctaag gaaaaaggcg taaatttctt attgcctaaa gatattatag ttgcggatga
     9601 tttcagtaat acggccaata ccaaagttgt ggcggtaaac gaaattccgg ctgactggga
     9661 aggcctcgat atcggacctg aaacctgtaa actgtatgcg gaaaccatta agaactccaa
     9721 gctggttgta tggaacgggc caatgggtgt gtttgaaatg gatctgtttg cagaaggtac
     9781 gaaagcagtt gcccaggctt gtggcgagac agaagcttat accattatcg gtgggggcga
     9841 ttctgcagcc gcggttgaga aatttaaaat ggcggacaaa atggatcaca tctccacagg
     9901 cggaggggct tccctggaat tcatggaagg caaagccctg ccgggtgttg tggcccttca
     9961 agataaataa aaccagagag gagtggcgag catgagaaag ccaattattg cagggaactg
    10021 gaaaatgttc aaaaccgtct ctgaagcggt ggaatttgca aacgcagtga aagggaaagc
    10081 agaaatgaac ggagtggaca gcgtggtatg tgcccctttc actaaccttc cggcgctagt
    10141 ggaaacgttc aagggaacct ctattaaagt cggtgcacaa aatatgcatt ttgaagacaa
    10201 tggagcttat accggagaaa tcagcggtat tatgctgaaa gatctcgggg tagaatatgt
    10261 gattatcgga cattcggaac gcagagccta t
//
