LOCUS       NZ_CP020557            10300 bp    DNA     circular CON 24-JUL-2024
DEFINITION  Paenibacillus larvae subsp. pulvifaciens strain SAG 10367
            chromosome, complete genome.
ACCESSION   NZ_CP020557
VERSION     NZ_CP020557.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus larvae subsp. pulvifaciens
  ORGANISM  Paenibacillus larvae subsp. pulvifaciens
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 4666454)
  AUTHORS   Dingman,D.W.
  TITLE     Paenibacillus larvae genome sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4666454)
  AUTHORS   Dingman,D.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2017) Entomology, CT Agric Expt Station, 123
            Huntington St, New Haven, CT 06504, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2016
            Assembly Method :: SMRT Pipe HGAP assembly v. February-2016
            Assembly Name                     :: sag10367-hgap-022916
            Genome Coverage                   :: 214.2x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002082155.1-RS_2024_07_24
            Annotation Date                   :: 07/24/2024 02:27:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,275
            CDSs (total)                      :: 5,166
            Genes (coding)                    :: 4,634
            CDSs (with protein)               :: 4,634
            Genes (RNA)                       :: 109
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 532
            CDSs (without protein)            :: 532
            Pseudo Genes (ambiguous residues) :: 0 of 532
            Pseudo Genes (frameshifted)       :: 400 of 532
            Pseudo Genes (incomplete)         :: 184 of 532
            Pseudo Genes (internal stop)      :: 26 of 532
            Pseudo Genes (multiple problems)  :: 72 of 532
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:07:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1788879
            Orig. end                         :: 1799179
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020557.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Dr. Adriana Alippi,
            CIDEFI-CIC, Facultad de Ciencias Agrarias y Forestales, Universidad
            Nacional de La Plata, 1900 La Plata, Argentina.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10300
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10300
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10300
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5300](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5300
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(361..717)
                     /locus_tag="B7C51_RS09535"
                     /old_locus_tag="B7C51_09535"
     CDS             complement(361..717)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639782.1"
                     /locus_tag="B7C51_RS09535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_09535"
                     /product="hypothetical protein"
                     /protein_id="WP_077996154.1"
                     /transl_table=11
                     /translation="MKSASKIIIGIVVGSLLVVGAVALLNGWIPVSRDTHPPCDQLPTV
                     AEATAALASHQGLAKEIKVLGDGIAVQVGKPCPDDQGRGLVMVSYGSRSERDAIADLLS
                     RRDGFGVPVHLVKR"
     gene            complement(714..1409)
                     /locus_tag="B7C51_RS09540"
                     /old_locus_tag="B7C51_09540"
     CDS             complement(714..1409)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014097336.1"
                     /locus_tag="B7C51_RS09540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_09540"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_077996155.1"
                     /transl_table=11
                     /translation="MLIEAQGVSVAIGGRPVLDGETVRCTPGIMTALVGPSGCGKTTLL
                     HCLGLLLPVDQGRILIDGDDVTRYGTAARRRFWRDHAAFVLQDYGIMDEESVAFNVTMQ
                     ASVLGRRVTGNQERLAQVLEQTGLQGREGELASHLSGGEKQRLALARAIYKDAAILFAD
                     EPTASLDAANRRKVIDLFADFAVRGRTVIVSTHDSEMINACGARHEVGSNSSSHHSSTS
                     DPASLGWSL"
     misc_feature    1554..1567
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.49"
                     /tool="antismash"
     gene            1690..1938
                     /locus_tag="B7C51_RS09545"
                     /old_locus_tag="B7C51_09545"
     CDS             1690..1938
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B7C51_RS09545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B7C51_09545"
                     /product="hypothetical protein"
                     /protein_id="WP_083039811.1"
                     /transl_table=11
                     /translation="MAGYQKVSPFHMDIYHEGNLIYRHKKALYQSDHQYIPEHYLKILE
                     RKPRAIINAAPLKGEVLPHELSEFMSFASTGIKTTSW"
     gene            complement(2268..2540)
                     /locus_tag="B7C51_RS27290"
                     /old_locus_tag="B7C51_09550"
     CDS             complement(2268..2540)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021219.4"
                     /locus_tag="B7C51_RS27290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_09550"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_367869905.1"
                     /transl_table=11
                     /translation="MPAHAWVNPGWHYQYPSEGGTWRYGFVDAGLRSEYNHPTKIHGST
                     VQRVIDGKVVSTNRSIDTAAGKYSHAYVGTINSPGLKAHYYYRTN"
     gene            3009..3176
                     /locus_tag="B7C51_RS25815"
     CDS             3009..3176
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B7C51_RS25815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_158530347.1"
                     /transl_table=11
                     /translation="MKKWMRKYREKSEFGLLDQRGRRQEYIDQDRYVNQLKRENEMLKK
                     CLEIKITVFG"
     gene            complement(3968..4552)
                     /locus_tag="B7C51_RS09565"
                     /old_locus_tag="B7C51_09565"
     CDS             complement(3968..4552)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016126149.1"
                     /locus_tag="B7C51_RS09565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_09565"
                     /product="recombinase family protein"
                     /protein_id="WP_083039813.1"
                     /transl_table=11
                     /translation="MIIGARVSAIDQNLDRQIGMLNEYGCEKIVKEKFTVTVKERKGLH
                     QLFDVIRKGDTVIVESISRLGRKTLDILNIIQQLEEMGVQFISLKENMDTRTSTGKAMF
                     QMMCVIAELERNLIAERVKEGLEASKRRGKTLGRPKLDKEKFAVALRMYDSKEYSIKEI
                     VSTTGISQGSLYRALVYTSYFDFITLFECIQ"
     gene            4664..4846
                     /locus_tag="B7C51_RS09570"
                     /old_locus_tag="B7C51_09570"
     CDS             4664..4846
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B7C51_RS09570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B7C51_09570"
                     /product="hypothetical protein"
                     /protein_id="WP_083039815.1"
                     /transl_table=11
                     /translation="MNSTMLFILTVVSMAIWVMVSRESMGELKDKGKNTGKMMTLIAAG
                     SLTTIFLLVSLYQKL"
     misc_feature    complement(4969..4985)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 22.57"
                     /tool="antismash"
     gene            5001..5300
                     /locus_tag="B7C51_RS09575"
                     /old_locus_tag="B7C51_09575"
     CDS             5001..5300
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343460.1"
                     /locus_tag="B7C51_RS09575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_09575"
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="WP_083039817.1"
                     /sec_met_domain="TIGR03651 (E-value: 4.7e-11, bitscore:
                     41.0, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINAKSVKNARSYALLSAGLLAITMFALTIGNGALIATQLGLSTT
                     TSIAIVKFLDAFSTVSYVITIIGLFSGVGTISSELAATILTILKKQGKAKAAAF"
     gene            5557..6429
                     /locus_tag="B7C51_RS09580"
                     /old_locus_tag="B7C51_09580"
     CDS             5557..6429
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B7C51_RS09580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B7C51_09580"
                     /product="hypothetical protein"
                     /protein_id="WP_235430706.1"
                     /transl_table=11
                     /translation="MRHLFFTITTGYSLQFLLAVLVSVTCMVVLHEYLIVFLALGSVVV
                     SFLSQLTSIFVKVIIRVIFLLQLWSILASLIGVSAILLAVQLLIIYFYISNYHRIPHGP
                     SFLKGSENRHASGSMSQIFLFYVINNKILLILLGAVVSVIMYFCQQLLNGVHGIPATIL
                     IYINFMTIVEILVGSKREEIMIDKSRVETLQSSLIVSPFKRFKASSIYLYSLMLMVTAA
                     LGLVGIFLNAADTTAIIKSVLSIPLTLLIGVIYYRKTELLTSGNESKILKLTLPILILI
                     FVTFFTIGA"
     gene            6953..7567
                     /locus_tag="B7C51_RS09590"
                     /old_locus_tag="B7C51_09590"
     CDS             6953..7567
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354993.1"
                     /locus_tag="B7C51_RS09590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_09590"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_083039819.1"
                     /transl_table=11
                     /translation="MNVEFIDCTKQFKDTHILDNVNFSIEPGLFHLTGRNGAGKSTLLR
                     MISGLDKQYSGRITTSEKSTLYLTVDPIGIHPFTIRENLEILWNTFGITPSAEQFAKVN
                     DFFDSKLDVPYGKASTGMKAKLGLSLLFVKDWEIVLIDEAMSTLDSESVEMLSERLVAL
                     SEMKVSIIIYVSHSMINKRLYDKSSAIHIEEGALSWKNIQN"
     gene            7546..7869
                     /locus_tag="B7C51_RS09595"
                     /old_locus_tag="B7C51_09595"
     CDS             7546..7869
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B7C51_RS09595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B7C51_09595"
                     /product="hypothetical protein"
                     /protein_id="WP_083039821.1"
                     /transl_table=11
                     /translation="MEKHPKLISFFSFILYVVLNYFFVSFATEHQIAGFSDEFAEVKKL
                     LADGDSLRQIITTIAAIVTTVSVLVQYIIGKFLLVVFVPGTKTHLYYALMPKLLVLMST
                     SFS"
     gene            complement(8197..8481)
                     /locus_tag="B7C51_RS28950"
                     /old_locus_tag="B7C51_09600"
     CDS             complement(8197..8481)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /locus_tag="B7C51_RS28950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_09600"
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="WP_083039823.1"
                     /transl_table=11
                     /translation="MFGIGFCKGLFEDLDSWIRRRLRMIRLRSWRKIKKLHKVLRRNDR
                     KGELPHLRMTKWRSSRSLPASVALPNEKFRQLRLVFLLDIYQDLHPQRG"
     gene            complement(8498..9178)
                     /locus_tag="B7C51_RS09605"
                     /old_locus_tag="B7C51_09605"
     CDS             complement(8498..9178)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /locus_tag="B7C51_RS09605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_09605"
                     /product="phosphatase PAP2 family protein"
                     /protein_id="WP_083039825.1"
                     /transl_table=11
                     /translation="MSRYSTRPTLHSVLLKDLVLGFAAAVFLLFVFLLISKSLASPWID
                     RFDSVISVWVQSYRGDVFNRILIGITKLGNGITETIVFIIVAGYLWVMHRRRKEAALLF
                     CNLAGSWLLNETLKVLFIRVRPDRIFFLVDATGYSFPSGHAMVSSSFYGFMVYLLWKHQ
                     RRKWRAAWLIPWLGAFVIFLIGFSRIYVGVHYPSDVLAGFAAGGIWLIGCIAGDKILQE
                     RSFR"
     misc_feature    9397..9413
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.8"
                     /tool="antismash"
     misc_feature    9755..9765
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
ORIGIN
        1 gccgccagca gcgcaagcag agtaaacaac gcacccgcga cagccactat gatacgctta
       61 tacataagca ggccctccaa gaactatctg gaacagtcgc ttggattagc aggtaatgtt
      121 ttttcattta tttcatcatc ctcatttatt cgactatttt tatcaatatt tttactattc
      181 aactaatttg tcaccgccca cctgccattc gccaaaggcg ccatgatgta cagccgagcc
      241 agcgcctcgt acttggcgta ggtggtcggc gccaggttcg gcttgacgac atcggcgagc
      301 cagccttaca cacaagcggc agacaacgat tgcgccgaac cggctgtgcg gaagtccgcg
      361 ttaacgcttc accagatgca ccggaacccc aaacccgtcg cggcggctga gcaggtcggc
      421 gatggcgtca cgctcggatc ttgaaccata gctcaccatg accagacctc ggccctggtc
      481 atcgggacac ggctttccca cttggaccgc aatgccatca cccagcactt tgatctcctt
      541 ggcgaggcct tgatggctgg caagcgccgc agtggcctcc gctacagtcg gcagctggtc
      601 gcacggcggg tgcgtgtccc ttgatactgg gatccagccg tttaggagcg caactgctcc
      661 cactaccagc agtgaaccaa caacgatacc gataatgatc ttagaggcag acttcataag
      721 gaccatccta ggctagcggg atcgctggtc gacgagtggt gtgacgagct gttggaaccg
      781 acctcatgcc gggcgccaca cgcgttgatc atctcggagt cgtgcgtcga cacaatgacc
      841 gtgcggccac gcacggcgaa gtccgcaaac agatcgatga cctttcgccg gttggctgca
      901 tcgagcgagg cggtgggctc atcggcgaac aggatcgcag cgtccttgta gatggcacga
      961 gccagcgcga gtcgctgctt ctcgccccca ctcaggtggc tggccaactc accttcccga
     1021 ccctggagac ccgtctgctc gagcacctgc gccaaccgtt cctggtttcc cgtgacgcgt
     1081 cgtcccagga cgctcgcctg catggtgaca ttgaaggcaa ccgactcctc atccatgatg
     1141 ccgtaatcct gaagtacgaa cgccgcgtga tcacgccaga accgacgccg tgccgcagtg
     1201 ccgtaccttg tgacatcgtc tccgtcgatc aagatgcgtc cctgatccac tgggagcaac
     1261 agcccgaggc agtgcaagag tgtggtcttt ccacatccgc tcggaccaac gagggctgtc
     1321 atgatcccag gcgtgcaacg aacagtctca ccgtcaagca cgggccgacc accaattgct
     1381 actgaaacgc cctgggcttc aatcaacatc tgcaccaccg atcaaattcg acgtctactg
     1441 aggacatcgc caacgcggcg aacgtcgctt tgatcgttcg agtcaagggt ttcctctttt
     1501 gcctatacaa aagtcttttc atttaattca ttcctcctca ttcattcgac tatttttgtt
     1561 aataaaaagg ttcatggatt cttagttttt acaggcaaat tttaacctag gatcctcaaa
     1621 aagaaatgat ccttaggtca cattttgtgg cctttttcat agatatgctc tattttttgg
     1681 tgtggtgctt tggcaggata tcaaaaggta tctcctttcc atatggatat ataccatgaa
     1741 ggaaatttga tatatcgtca caaaaaagcc ctttatcaat cagatcacca gtacatccct
     1801 gaacattatc tgaaaattct ggaacgaaag ccccgggcga ttataaatgc tgcgcccctt
     1861 aaaggagagg tattacccca tgaactctca gagtttatga gctttgcaag caccgggata
     1921 aaaaccacca gttggtaaat atcctgcttc tggggaaaaa accgctcccg caccgacatg
     1981 gtaggtgggc ggtaaggcaa gctaatttga taggtactcc aacctatgac ctggtatgtt
     2041 tttatctaga aattaaagca gaaagcagat ttaactctct ggacaccaat ggcctgcgga
     2101 aagtctcgat catataggtt ggtgatgatc gctgccagca gcgcggggca gagtaaacaa
     2161 cgagcccgcg acagccacta taataagctt atacaaaagc gggaccccaa gaactatctg
     2221 gaactcgcca tgctaagccc cggcgaccgc aaccagtatc gcctggatta gttggtacgg
     2281 tagtagtaat gagccttcag acccggcgag tttatggttc cgacataggc gtgagagtat
     2341 ttccccgcag ccgtatctat gctcctgtta gtacttacca ccttgccgtc gatgaccctt
     2401 tgcaccgtcg agccgtggat cttcgtcggg tggttgtact cggaacggag tccggcgtcc
     2461 acgaatccgt atctccatgt tccaccttcc gatgggtact ggtagtgcca acccggattc
     2521 acccaggcat gggcaggcac cgcggcgccc ccaccaaagc gatggatgtc gccaacgcgg
     2581 ctaacgttgc tttgatcgtt cgagtcaagg gtttcctctt ttgcctatac aaaagttttt
     2641 tcatttagtt cattcctcct cattcattcg actatttttg tcaatatttt tactcttcaa
     2701 gctaatttgt caccgctcac taccatgccg atgcgggagg gtttcttgcc ctttttggcg
     2761 atgagttaca tttaatgtaa taaattcaat aaagaaaata gaagttcctt ttttttcaag
     2821 tatattaatg aggagttgta tataaaatct aaactcattg caaagggggt gcatttatat
     2881 ggctatcaaa ggacagaagt tcaaaacgta ttcagaagaa ctaaaggctg aggccatgtc
     2941 tgcatttgga ggaaaagtga acttatcagc agatccatga atatttcggg attcaggata
     3001 agcagcgaat gaagaaatgg atgaggaaat atcgagagaa gagtgaattc gggcttctgg
     3061 atcagcgggg aaggcgccag gagtatatcg accaagatcg ttacgtcaat caattaaaac
     3121 gggagaacga gatgctaaaa aagtgtttgg agattaagat cacggtgttt gggtaaaacc
     3181 ataagaaggt cctgcgcctg atacaggcta tgaatttacg atctattatc cttcgaaaga
     3241 cgtcgttgta actattcctc atccacaggt accgatcgtt tccactgatt tgaacggaac
     3301 ggttcatcta agaacaaaga tgtttctttt gtacctctca aggttaacgt gtaggctcgg
     3361 tgatttttcc ccaaaaatgg cagggacaca tgttaaatcc acccctgctt ccataggaga
     3421 ctcatcgggt tgtagatcat acgaagatcg taattgaaat aagctcctaa ttgctcagcg
     3481 gtcattagta gatattgata cagggtcacc atgatttctt ttatgtttga ttcatgtgat
     3541 ccccatccat ttcaccccct tttttttcct caaagcagag aaatacggaa acaaaaaccg
     3601 cccggcaaag ggcggtccaa aacgcgcgtt ttttagctgc tcaccttgac tctattctat
     3661 attaacatta agcctcaact aaaagtctgc tcaagaaaca tttccaagaa aaaacgatcc
     3721 agacgtgttt ttacaaccgt atttattcca acgattagat cctttagctg ctgattcatc
     3781 ctccatctct gaggtgatcc gttcattcaa tcagatatcc aggcaactcg ttcgcgggga
     3841 aaataaagat tggcgttttc ttgaaccggt aataagttgc aaagtaaaat ttcaatgttt
     3901 caaaaaagac ggtaagctgc gctcccccaa atttggggaa ttctgtacac ctatttctgt
     3961 gcaaccccta ttgaatacat tcaaatagag tgataaaatc gaagtaagaa gtatatacta
     4021 atgctctata taaggaccct tgagatattc cagttgttga gactatctcc tttatagaat
     4081 attctttact atcatacatc cgtagcgcaa cagcaaactt ttctttatcc aatttgggtc
     4141 tacctaatgt ttttccacgt cttttacttg cttcaagtcc ttccttaact ctctcagcaa
     4201 taaggtttct ttctaattca gcaatgacac acatcatttg gaacatggct ttgcccgtgg
     4261 aggttcttgt atccatattt tcttttaaag aaatgaattg tacccccatc tcttctaact
     4321 gttgaataat gttgaggata tctaatgttt tacgtcctaa acgggagatg ctttccacta
     4381 taactgtatc gcctttccgg atgacatcaa aaagttgatg aagtcctttt cgctctttaa
     4441 ccgtaacagt aaatttctcc ttaactattt tttcacaacc atattcatta agcataccaa
     4501 tttgccgatc taaattttgg tcaatagcag acacccgagc accgattatc atttgtatcc
     4561 tccaataaac ttgtatattt ttattttact agagaggcac ttattgtaaa ggtatttggt
     4621 aatacttttg gtaaggggat tacaatattt ggaggtgtag tagatgaatt caactatgct
     4681 ttttatttta acggtagtgt ctatggccat ttgggtcatg gtttcccgag aatctatggg
     4741 tgagttaaaa gataaaggaa aaaatacagg taaaatgatg acgctgattg cagcaggtag
     4801 tttaactacc atatttttat tagtctcact ctaccaaaaa ttgtagtggt gaaggtctat
     4861 gatcagcccc ttttaaaccg tgccaagcta aacgaaaaat agaaatgctg atacatattt
     4921 caagggataa gctttgcaaa attgtttcat agtttggtgt atttttcata gtaagtaaat
     4981 aacaattagg aggttttatc atgataaacg caaaatctgt aaaaaatgca agatcttatg
     5041 cgcttttatc cgcaggactt cttgccatca caatgttcgc tttaaccatc ggtaacggag
     5101 ccctcatcgc gactcaacta ggtcttagta cgacgacatc aatagcaatc gtcaagtttt
     5161 tagacgcatt ctcaacggtc tcatatgtaa ttacaatcat tggtcttttc agcggtgtgg
     5221 gaactatttc gtcagagctt gccgcgacta ttcttactat ccttaaaaaa caaggaaaag
     5281 ctaaagcagc agctttctaa tcacacaatg acacagagat tatttaaatt acatgacaaa
     5341 agcatattgg gtcatctcaa ttatattggg atgattcttt tttcatcatt agttattata
     5401 gccctgactt tctatcatga tttggccccc acccgttacc tagtcttgta ttcttttatt
     5461 tgcatatacg tgatagggac agcaaatatc aatcgttgca tgttacccta tggaaaacaa
     5521 aaaccgggct tggtgtatat ttctaagcgg ctgctattga gacatttgtt ttttacaatc
     5581 acaacaggat attccttgca attccttctg gctgttttag tatccgtcac atgcatggta
     5641 gtattgcatg aatatcttat cgtcttttta gctttaggtt cagttgtcgt ttcgtttttg
     5701 tctcaactga catccatatt cgtcaaggtt attatcagag tgatcttctt gttgcaattg
     5761 tggtccattc tggcatctct aattggcgta tccgccattc tactggctgt acagttgctt
     5821 atcatctatt tttacataag taattaccat cgcatcccgc acgggccgtc attcttgaag
     5881 ggctctgaga atcgacatgc ctccgggagc atgtcgcaga tttttttatt ttatgtaatc
     5941 aataataaga tcctattaat cttgcttggc gcagtcgtat ctgtaatcat gtacttctgc
     6001 caacaactat taaatggcgt acacgggatt cctgcgacga tccttattta tataaatttc
     6061 atgacgattg tggaaatttt ggttggctca aaaagggaag aaatcatgat cgataaatca
     6121 agggtagaaa cgctacaatc ttctttaatc gttagcccat ttaaacgatt taaagcaagc
     6181 tctatctatt tatactcatt aatgctgatg gtcaccgcgg cattgggatt agtcgggatc
     6241 ttccttaatg ctgccgacac gaccgcgatc atcaaaagcg tcctgtcgat tccattgacc
     6301 cttctgatag gggttatcta ttacagaaaa acggaattgt tgaccagtgg caatgaatcc
     6361 aaaatattaa agctgactct acccatttta atcctaatat ttgtcacatt tttcactatt
     6421 ggcgcatgaa aagaggcgta agaatgacgc agcctaaatc caaaattttc cttgtgtact
     6481 atatgatttt ttgtatatcc gttgttggta taggtgttta tttagcatct gtacttgata
     6541 cggatttatt tggggtgaac cctccagctt tgcagaatta gttgatgtat cttgttttat
     6601 cgcacaacat aagaaacttc gtcatgtatt tattggcttt tccgatttcg ccatttctcc
     6661 agttattcga ttttggaggt tccgcttttc aaatcgcaat gtcgtatcgg attcaaggac
     6721 ctgatgcgac cataagcaga cttatcccac acggcttatt ggagtttccg aacatgttgt
     6781 tttatcaagg gatgagccag tatgtgctat tcacattgat tactacgaga tccatcaagg
     6841 ctgctttagg tttaatgaaa aaaatgatcc ctctttatgt gctgtcaata atcgtattaa
     6901 taattgcagc aatgcttgaa ggtcatttct aaataaagaa aaggggattc ctgtgaacgt
     6961 agaatttata gattgcacaa aacaattcaa agacacgcat atactagaca acgtgaattt
     7021 ttccatagaa ccaggtcttt ttcatctgac agggcgaaat ggtgcaggga aaagcacgct
     7081 cctcaggatg atttccggcc ttgataagca atattcagga cgtattacca ccagtgaaaa
     7141 atctacatta tatttaacgg ttgatccgat cggcatccat ccttttacga ttagagaaaa
     7201 ccttgaaatc ctgtggaata cgttcggcat tacgccatct gcagagcaat tcgccaaggt
     7261 caatgacttt tttgatagca aattagatgt tccatacggt aaagcatcca cggggatgaa
     7321 ggcgaagctt ggcttatctt tactttttgt gaaggattgg gagattgtat tgattgatga
     7381 agcgatgtca acattggatt cggaaagtgt tgaaatgcta tctgaacgtt tagtagctct
     7441 atcagaaatg aaagtatcga ttatcatata tgtgtcccac agcatgataa acaaaaggtt
     7501 atatgataaa tcgagtgcta ttcatataga ggaaggagca ctttcatgga aaaacatcca
     7561 aaactaatat cgtttttctc attcatcctg tatgtagtgc tcaattattt ctttgtatct
     7621 tttgccaccg aacaccaaat agccggattc tctgatgaat tcgccgaagt gaaaaaactt
     7681 ctagcagatg gcgatagctt gcgtcaaatc ataacgacca tcgcagcaat cgtaacgaca
     7741 gtgagtgtac tcgtccaata tatcatcggg aagtttctat tggttgtttt tgttccaggt
     7801 acgaaaacgc atttatatta tgcgctgatg cctaagctgc ttgttctgat gtcaacatca
     7861 ttttcatagg ggttttcaac atacaaaata cgtggttata tctatcgaca gccctcatag
     7921 gagcgctttt gatcatgttc tttattcaat ataaaaggaa taactggaaa gctactatcc
     7981 ttttttcctt ggtttttttg ctagactccg tattttcatt aggaaaatcc atttattctt
     8041 attttaggac gtttccggca catacaccta cctctattct catggtgatg gatgggtaat
     8101 aaaaaaagcg atgcgtaatt tactaaccgc atgaataatt tcatgcgcct ctcacagatc
     8161 catacgtgcg ggtcatcgca tacggctcct ccatgtttat ccccgttggg gatgtaggtc
     8221 ttgataaata tccaacagaa aaactaatcg gagttgtcga aatttttcat ttggtagtgc
     8281 aacacttgca ggaagactac gagagcttcg ccattttgtc attctgagat gaggaagttc
     8341 tcctttccga tcatttcttc ttagcacttt gtgaagcttc tttattttcc gccaactccg
     8401 taaccgaatc attcgcagtc ttcgccttat ccatgagtcc aaatcttcaa ataaaccttt
     8461 acagaatcca atcccaaaaa tagctccccc aaccccttca ccgaaacgac ctttcctgca
     8521 aaatcttgtc tcccgctatg catccgatca gccaaatccc tcctgcagcg aagcctgcca
     8581 gaacatcact tgggtaatgg accccgacat agatacggct gaagccgatc aaaaaaataa
     8641 cgaatgcccc gagccaaggt atcaaccaag cggcacgcca ctttctcctc tggtgtttcc
     8701 atagtaaata gaccataaag ccgtaaaagg atgaggaaac catggcatga ccacttggaa
     8761 aactgtaacc cgtagcatca accagaaaaa aaatgcggtc cggcctgacc cggataaaaa
     8821 gcacctttag cgtctcgttc aacagccagg agccggccaa gttgcaaaac agcaaagccg
     8881 cctcttttct tcggcggtgc attacccata aatagcccgc aactatgatg aaaacgattg
     8941 tttcggtaat tccgttcccc agcttcgtaa tcccaatcaa aatacggtta aacacatcgc
     9001 cccggtagga ttgaacccag acggaaatca ctgaatcaaa ccggtcaatc caaggagagg
     9061 ccaggctttt cgagattaac agaaaaacaa atagcagaaa aacagcagct gcaaaaccaa
     9121 gcacaagatc tttcagtaaa acagaatgca atgtcggtcg ggtagaatat cgggacaatg
     9181 ggcttatctc ctttgttcaa atataagcat atattgtgca ttgtataaag agataagatg
     9241 gaaatcaaac atcccgcttc agaacctatg aataaatagc gctaccttgc cccctgaact
     9301 cctctgcctt ttgcttcatc cccgctttca aagcctcatc ctcctgaagc cccttttccg
     9361 cggcatattc cctgatatcc tgtgtaattc tcatactgca gaatttcggt ccgcacatgg
     9421 agcaaaaatg ggctgttttg gctccttcgg caggaagggt ctcatcgtga tactccatcg
     9481 ctctttccgg atccagggaa agctggaatt gatcccgcca acggaattca aaccgcgctt
     9541 tggagagagc atcgtcgcgc cttcccgcat gcggatgccc tttggccaag tccgcagcat
     9601 gagcggctat tttataagct atgactcctt cccgcacatc atctttatta ggcaggccca
     9661 gatgttcctt cggggtaacg taacagagca tagcagttcc aaaccagcca atcatggctg
     9721 ccccaattgc cgacgtaata tgatcataac ccggtgcaat gtcagtagtg agcggcccta
     9781 atgtataaaa tggggcttcc ctgcagacct tctgctggcg gtctacattt tccttaatca
     9841 gatgcatagg aacatgccct ggtccttcta tcatgacttg aacatcgtgt ttccatgcga
     9901 tctctgccaa ttcccccaat gtatccagtt cggcaaactg ggcttcgtca ttggcatcag
     9961 caatggaacc cggacgaagg ccgtcgccta aagagaaggc tacatcatat gttttcataa
    10021 tttcgcaaat ctcttcaaag tgtgtataca ggaaattctc ctcatggcag gccaagcacc
    10081 aggcggccag aatggaacct ccacgggaga cgatccccgt tgttcttttt accgtcagag
    10141 gaatgtaagc gagccggaca cccgcgtgaa tggtaaagta atccacaccc tgttccgctt
    10201 gctcgatcag agtatcccgg tagatttccc aggtcaaggc ttccgcttct ccatttacct
    10261 tctctaaagc ctgatatata ggtacagttc caataggaac
//
