LOCUS       NZ_CP020557            10186 bp    DNA     circular CON 24-JUL-2024
DEFINITION  Paenibacillus larvae subsp. pulvifaciens strain SAG 10367
            chromosome, complete genome.
ACCESSION   NZ_CP020557
VERSION     NZ_CP020557.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus larvae subsp. pulvifaciens
  ORGANISM  Paenibacillus larvae subsp. pulvifaciens
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 4666454)
  AUTHORS   Dingman,D.W.
  TITLE     Paenibacillus larvae genome sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4666454)
  AUTHORS   Dingman,D.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2017) Entomology, CT Agric Expt Station, 123
            Huntington St, New Haven, CT 06504, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2016
            Assembly Method :: SMRT Pipe HGAP assembly v. February-2016
            Assembly Name                     :: sag10367-hgap-022916
            Genome Coverage                   :: 214.2x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002082155.1-RS_2024_07_24
            Annotation Date                   :: 07/24/2024 02:27:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,275
            CDSs (total)                      :: 5,166
            Genes (coding)                    :: 4,634
            CDSs (with protein)               :: 4,634
            Genes (RNA)                       :: 109
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 532
            CDSs (without protein)            :: 532
            Pseudo Genes (ambiguous residues) :: 0 of 532
            Pseudo Genes (frameshifted)       :: 400 of 532
            Pseudo Genes (incomplete)         :: 184 of 532
            Pseudo Genes (internal stop)      :: 26 of 532
            Pseudo Genes (multiple problems)  :: 72 of 532
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:07:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 96443
            Orig. end                         :: 106629
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020557.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Dr. Adriana Alippi,
            CIDEFI-CIC, Facultad de Ciencias Agrarias y Forestales, Universidad
            Nacional de La Plata, 1900 La Plata, Argentina.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10186
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10186
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10186
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5186](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5186
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(336..1625)
                     /locus_tag="B7C51_RS00595"
                     /old_locus_tag="B7C51_00595"
     CDS             complement(336..1625)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1273: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919036.1"
                     /locus_tag="B7C51_RS00595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00595"
                     /product="hemolysin family protein"
                     /protein_id="WP_083038083.1"
                     /transl_table=11
                     /translation="MGVNLIMVAILILLTAFFVATEFAIVRLRSSRVDQMVFEGKKNAV
                     AVQKVTGNLDEYLSACQLGITITALGLGWLGEPTVEKLLHPLFGILHLSGNWTRILSFL
                     ISFILVTYLHVVLGELAPKTIAIQKAEQISLLTAKPIIYFYKILYPFIWLLNGSANLLI
                     KLFGLKPVSEHEEAHSEEEIRIILSESYESGKINKTEFGYVNRIFTFDELLAKEIMVPR
                     TDMVCLFTNHSLKENMDTIRQEQYTRFPVAEGSKDNIIGMVNTKQFFLNYDPQKPFDFK
                     SLIHPILMVPDVMPVKSLLKKMQQERVHIALLLDEYGGTAGLITIEDILEEIVGEIRDE
                     FDEDEPQEIEQINETTYLVDGKVSLDRIQPLFGVEFDAEEVDTIGGWLYSQNQFLKENV
                     GWTYSHLTFTIRERDKNRIRKIEIRTSQTK"
     gene            1911..2666
                     /locus_tag="B7C51_RS00600"
                     /old_locus_tag="B7C51_00600"
     CDS             1911..2666
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447232.1"
                     /locus_tag="B7C51_RS00600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00600"
                     /product="DUF1836 domain-containing protein"
                     /protein_id="WP_083041501.1"
                     /transl_table=11
                     /translation="METFTLTRKEISGLLRCLQGHSEKTPLQVLQQAWASRHQQDLEAG
                     TSFAAFVSTALPSIFTKLIKTQPVHHLSLHDIVALGNQIEYTTFSITSVQNWVKRDFKE
                     MLVKNKKGRKYCVNQIALLFMIEDFKTNLDFESIRGLFRAVFGDFRTDEDDLLEPVDIY
                     AIYANMAEEIETDKTQHMEQVIRNKADRFVHSLVHLNEEQKEAVRNMIMIGVISIQTAF
                     CQHLAKQYINATLFLQHLHKGKGLKKHKR"
     gene            complement(2763..>3281)
                     /locus_tag="B7C51_RS00605"
                     /old_locus_tag="B7C51_00605"
                     /pseudo=""
     CDS             complement(2763..>3281)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033862 - UMP kinase activity [Evidence
                     IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /GO_process="GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423360.1"
                     /locus_tag="B7C51_RS00605"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00605"
                     /product="uridine monophosphate kinase"
                     /pseudo=""
                     /transl_table=11
                     /translation="DRIHMREAGVLKSKTEKEVRVMSAMPVNTVAEPYIRLRSTHHLEK
                     GYIVIFAGGNGQPYVTTDYPSVQRAIETNSCAILVAKHGVDGVFDHDPRARTDARKYAS
                     LPYDEVLERNLKVMDQSAFILAREYKLPIHVFDFDQTGSMKAICEGNHVGTFIGENTAV
                     EYAESTIVT"
     gene            complement(3366..3956)
                     /locus_tag="B7C51_RS00610"
                     /old_locus_tag="B7C51_00610"
     CDS             complement(3366..3956)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016508801.1"
                     /locus_tag="B7C51_RS00610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00610"
                     /product="YitT family protein"
                     /protein_id="WP_237089117.1"
                     /transl_table=11
                     /translation="MGIFVLAFGSAAMIQANLGVSSWDVLHIGMTNWTPLSIGTWVQIV
                     GLLMLGGSSWLDRTWPQLGSIVNIVLVGFFLNWILGLHLIPTVQLLWERGLLLVAGILC
                     MGFGSGMYVASNIGAGPRDGMTLVLARKFGLSIRLVRTLLELTALTIGWIAGGPVAAGT
                     FISVFLIGPVMQASLAFWRRRLVHDKSEPGKGA"
     gene            complement(4108..4542)
                     /locus_tag="B7C51_RS00615"
                     /old_locus_tag="B7C51_00615"
     CDS             complement(4108..4542)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119089.1"
                     /locus_tag="B7C51_RS00615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00615"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_023485119.1"
                     /transl_table=11
                     /translation="MIYGHTVNRTARKFSKYLNELLDPLGLFSSQWGVILCLHDRGPCS
                     QTELCEYLSVEAPTMTRTLARLEKIGYVERRRAKDRRAYHVSLTPHAMEMLGVWTQASE
                     MTESIALKGIDDKDLEVFNKVLDQMNRNLDRQFAKVPKET"
     misc_feature    4664..4679
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 24.77"
                     /tool="antismash"
     gene            5001..5186
                     /locus_tag="B7C51_RS00620"
                     /old_locus_tag="B7C51_00620"
     CDS             5001..5186
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF020782.4"
                     /locus_tag="B7C51_RS00620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00620"
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="WP_083038087.1"
                     /sec_met_domain="TIGR03651 (E-value: 1.1e-09, bitscore:
                     36.7, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNISWQSASRVIDAVNAGASIWGIVSIIVAGGGILGMGYAALALL
                     VKKKVKDLGETAAIAW"
     gene            5311..6999
                     /locus_tag="B7C51_RS00625"
                     /old_locus_tag="B7C51_00625"
     CDS             5311..6999
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B7C51_RS00625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B7C51_00625"
                     /product="hypothetical protein"
                     /protein_id="WP_083038088.1"
                     /transl_table=11
                     /translation="MKLTGGFIISNLVRIVYYQHMLHYRWLLDHFIALTDSRLVYAKLL
                     LFLFPFTLGAFSFYISKGIFSYTLSLEWVPARSTLEALFMIVFFLLFWVLLFRDYKHNY
                     ARAFYSHDSTLYLAQGIHFILILTAKLIDVFFLSVWLFIYPILIGISLAWNSVYSIKIP
                     LQWFTIDFGLLLVLILLLRNLSMIIMFLVKSTKKQRGQTYWFQHLHVLALGKYIFIGIG
                     SYIVTILFIQTHTPAWSWQSLQSEHLNLFHKLSFPTQWLITGKEFYFLLLLFSLLITFV
                     TLVIGFNRILYVKSQLTTRYVNRDYSSGLRLLETPNMPNWLLLIMKDLIIVWRDKRTLM
                     TPVGQFLSMFMAFLGTLGALRNSELFHSQTVSLSAIIAMQFIFSTFAGNMISRITSIDA
                     EGRMISTLLLYLRSPAVFMKSKLILHSLFVGGVITSVTILLSLMFRIEVTLFLYSLCSS
                     LLITMTHSLSFIVSSAIFPKFNWDHETRIGTSSKAVLLESLFLRVYEFGCMISIAVAAV
                     YLYTNALSPNQYYICILCIIILFSSVWISILLLLYRIPWWKGWKF"
     gene            6996..7739
                     /locus_tag="B7C51_RS00630"
                     /old_locus_tag="B7C51_00630"
     CDS             6996..7739
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="B7C51_RS00630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00630"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_158672760.1"
                     /transl_table=11
                     /translation="MNPIEVYGISYQYVGTKDLAVKDITFHVKSNEIVGLLGHNGSGKT
                     TIMNCIAGLFQPVQGKVLLSGTQISDLCKPKPVGYVPADPILYNMLTVQEYIVFIGKLH
                     HLSTAQIHTISEPLLDMLKLKDNRHKLIKHLSHGMKQKVSMITGLLHQPPALVLDEPMT
                     GFDAQSTKEMKDFLVHLVRTNHGAGILLSSHRLDIVESICDRIVVIRQGQLIFTGSVQE
                     LKHSAQHGNSLEEAFLSITSENGGN"
     gene            7740..7934
                     /locus_tag="B7C51_RS00635"
                     /old_locus_tag="B7C51_00635"
     CDS             7740..7934
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B7C51_RS00635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B7C51_00635"
                     /product="hypothetical protein"
                     /protein_id="WP_024093949.1"
                     /transl_table=11
                     /translation="MRGKQVVNQISLIFIFAFIGIYGDIQWYQKGNDASLFIMGMALFG
                     TSYAIVLIVDPIVGWLKKK"
     gene            7985..8290
                     /locus_tag="B7C51_RS28635"
                     /old_locus_tag="B7C51_00640"
     CDS             7985..8290
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /locus_tag="B7C51_RS28635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00640"
                     /product="stage II sporulation protein M"
                     /protein_id="WP_367869910.1"
                     /transl_table=11
                     /translation="MIYFLSTCIGFFSGPYLQLNPIALSLSTNPYTYIIHNFKSMVFFI
                     SGLFTMGISTMYVLFMNGFVLGASLYGIGTASTFFSAFIRIIPHGIFEQEEGVYLP"
     gene            complement(8421..9625)
                     /locus_tag="B7C51_RS00645"
                     /old_locus_tag="B7C51_00645"
                     /pseudo=""
     CDS             complement(8421..9625)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006836507.1"
                     /locus_tag="B7C51_RS00645"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00645"
                     /product="MDR family MFS transporter"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPKKLCLLVMGMFINVTGASFIWPLNTIYIHYHLGKALAVAGFVL
                     TLNSFASVVGSLLGGYLFDRIGSYRTVVQGVVLTLAAATVLAFNHDWWFYVVCLIVMGL
                     GSGMVVPSIYASAGLIWPSGGSKTFAAIYVAQNAGVALGSALGDISLHIHSTIYSGEMP
                     LSMPYLLCWWCLHSNVFQRPQVPSAKKKEV"
     misc_feature    complement(9581..9599)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 25.22"
                     /tool="antismash"
     misc_feature    complement(9584..9599)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.09"
                     /tool="antismash"
     misc_feature    9734..9747
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.72"
                     /tool="antismash"
ORIGIN
        1 ccatatattg ggatatgaat gcacatattt caaccttgaa tagattcggt aaaatatacc
       61 tgttccccat aagaaaatcg agctcaacgg gaatgcctac aatatcgccg tctatgggga
      121 tgcccgccat tatgcggctt tattctggga tgaggtggag aaagagttag aaattcatga
      181 gacaagaaac cttggccgtt gcctttttgg agcacatgcg ggaaattatt gctgtttaaa
      241 ttcaatgata aaagccgctg tgtacgaact gcaccccaat tgttggatgc aactaacaat
      301 tggaggtgca tttcagtatg tcagcggctt tcactttatt tagtttggct agtccggatt
      361 tcaatcttgc ggatccggtt tttgtcacgt tcccggatag taaaggttag atgactgtat
      421 gtccagccga cattttcttt aagaaattga ttctggctgt acagccagcc gccaatggta
      481 tccacttctt ccgcatcaaa ttcgactccg aacaaaggct gaatccggtc aagcgatacc
      541 tttccgtcca ccagataagt ggtttcattg atttgttcaa tctcctgagg ctcatcttcg
      601 tcaaattcat cacggatttc gcctacgatt tcttccaaaa tatcctcaat ggtaatcagt
      661 ccggccgtgc cgccatattc atcaagcagc aaggcgatat gcacgcgttc ctgctgcatt
      721 ttcttaagca gtgatttaac cggcatgaca tcaggcacca tgagaatcgg atgaatcaaa
      781 gatttaaaat caaacggctt ttgaggatcg tagttcagaa aaaattgttt cgtattgacc
      841 atcccgataa tgttgtcctt actgccttct gctaccggaa aacgtgtata ctgttcctgc
      901 cggatggtat ccatgttttc cttcagggaa tggttcgtaa acaggcagac catatccgtc
      961 cggggtacca tgatttcctt ggcgagcagt tcatcgaatg taaaaattct gtttacataa
     1021 ccgaattcgg ttttgttgat tttaccgctc tcgtaacttt cagataaaat aatgcggatt
     1081 tcttcttccg aatgggcttc ttcatgttcg ctcactggct ttaagccaaa caatttaatc
     1141 agcagatttg cggagccgtt aaggagccaa atgaacggat aaagaatttt ataaaaataa
     1201 ataataggtt tggccgttaa taaactgatc tgctcggctt tttgaatggc aatcgtctta
     1261 ggggccagct ctcccaatac cacatgaagg taagttacta atatgaatga aatcagaaaa
     1321 gagagaatac gggtccagtt tccggacaaa tgcaggatgc caaataaagg gtgaagcagt
     1381 ttttcaacag tcggttctcc cagccagcca agacccaagg ccgtgatcgt aatccccagt
     1441 tgacaagcgg ataagtattc gtccaggttg cccgtcacct tctgaacagc tactgcattt
     1501 tttttgcctt caaacaccat ctggtctacc cgacttgaac gcagtcttac aatcgcaaat
     1561 tccgtcgcca caaaaaaagc cgttaacaaa attaaaatag cgaccataat caaattaaca
     1621 cccattcatc aaccatcctt taattattta gcattttgcc tgatttcata gactccaggg
     1681 gcagcaaaac gctgatgtta gaacttttga ttagttctta agaacttatt ttatgattaa
     1741 ttcctttacc tgacaaattt ctctatgctt caaaaaatcc cccatcggcc gatctgggca
     1801 tgtttagcag ttaaacgaaa ctccgcacgt ttgttttgcg cttttgacaa tcgctcgtac
     1861 tgtatttaag atgtaggcat atgcagaagg cttaaagtga ggtgatcaag atggaaacat
     1921 tcacattgac gcgtaaagaa atatccggat tgctccgttg tctgcaggga cactcagaaa
     1981 aaactccctt gcaggttctt cagcaggcgt gggcaagccg ccatcaacag gatctggagg
     2041 cggggacttc ttttgcagcg tttgtttcca ctgcccttcc ttctattttt accaaactga
     2101 tcaaaacaca acccgtacat catttgtcct tgcatgatat tgtagctctg ggcaatcaaa
     2161 ttgaatatac gacgttctct attacttccg tccaaaattg ggtgaaaagg gatttcaaag
     2221 agatgttggt gaaaaataaa aaaggcagaa aatattgtgt caaccaaatc gccctgctgt
     2281 ttatgatcga ggatttcaaa acaaatttgg attttgaatc gatacgcggc ttgttccggg
     2341 cagttttcgg agattttcgg acggatgaag acgatttgct ggagcctgtt gacatttacg
     2401 caatttatgc aaatatggca gaagaaatag aaacggataa aacccagcat atggagcaag
     2461 tcatccggaa taaagctgac cggttcgtcc attctcttgt tcatctgaat gaggagcaaa
     2521 aggaggccgt acgcaacatg attatgatcg gagtaatatc tatacaaact gctttctgtc
     2581 aacatttggc taagcagtac ataaatgcca ctttgttcct gcagcatttg cataagggta
     2641 aaggtttaaa aaagcataaa agatgaggcg ccttcagcct catcttttgt aaagtttcgg
     2701 ccatctttcg tgccggaatg aacgtgttga ggaaaaccgt ataatcgaat atatcgtaaa
     2761 cattaggtaa ctattgtact ttccgcatat tccaccgctg tattttctcc tataaacgtc
     2821 ccgacatgat ttccttcaca aatggctttc atagatccgg tctggtcaaa gtcaaacaca
     2881 tgaatcggga gtttgtattc tcttgccaga ataaaggccg attgatccat gaccttcaga
     2941 ttccgttcca gtacttcatc gtatggaaga gaggcatact tccgggcatc cgttctcgct
     3001 cgcgggtcat ggtcaaatac cccgtccacc ccgtgtttgg ctactaatat ggcacagctg
     3061 ttcgtctcaa tagctctttg aaccgaagga taatctgtgg ttacataagg ctggccgttt
     3121 ccacctgcga aaataacaat atagcccttt tccaaatgat gagtcgaccg cagccggata
     3181 tagggctcgg ctaccgtatt gacgggcatc gccgacatga ctctgacttc cttttccgtt
     3241 ttggatttca gcacaccggc ttcccgcata tgtatccggt ctcttcacga atacaacttg
     3301 attcatcctt gattctcctc ctgttggata tccagtttcc cagttttgaa agttcggaac
     3361 ttatattatg cccctttacc cggttcgctt ttgtcatgaa caagtctcct tctccaaaaa
     3421 gcgagcgatg cctgcatcac cggaccgata agaaataccg atataaacgt acctgccgca
     3481 accggcccgc ccgcaatcca gccgatcgtg agggccgtta attctaatag ggtccgcacc
     3541 aaacggatgg aaagcccaaa ctttcgcgcc agaaccagcg tcataccatc ccgcgggcct
     3601 gcccctatat tggatgctac atacatacct gatccaaaac ccatgcaaag aataccggca
     3661 acaagcaaga gtcctctttc ccacagcaat tgtacggttg gaatcagatg caggcccaga
     3721 atccagttta ggaaaaagcc gaccagaaca atattgacaa tacttccaag ttggggccaa
     3781 gtccggtcca gccaggacga tccccctaac ataagcaggc cgacaatttg tacccacgtt
     3841 ccaatcgata gcggagtcca gttcgtcatg ccgatatgaa gcacatccca ggaagacaca
     3901 cctaaattgg cctgaatcat agcggctgac ccaaaggcca gtacaaagat tcccacaaca
     3961 tacaaagcat acctctgaaa ggttattttc caaaccgttc tactcccctg atctcgtcta
     4021 ttctccactc tcaatttcca gcgcctcttt ctataattct ttattttgat tgtcaaatca
     4081 aacattttga ccgtttctct taccttttca agtctcttta gggacttttg caaattgccg
     4141 gtctaaattc cggttcattt gatccagtac tttattaaac acttctaagt ctttgtcatc
     4201 aattccctta agagcaatcg actcagtcat ttccgaagcc tgggtccaga ctcccagcat
     4261 ttccatggca tgcggcgtta agctgacatg ataggccctg cggtcttttg ctctccgtcg
     4321 ctccacatag ccgatctttt ccagccttgc aagtgtccgg gtcattgttg gagcttcaac
     4381 agataaatat tcgcatagtt ccgtttgtga acaaggtccc ctgtcgtgta aacataaaat
     4441 aactccccat tgggagctga acagtccaag aggatcaagg agttcgttta aatatttaga
     4501 gaactttctt gcggtccgat ttactgtatg gccgtaaatc atggattgga atcccttctc
     4561 tcttccgcat attagttact tagctaagta tatacaagac cggttatcct gtcaatacaa
     4621 acaaaaaagg aaggaacagc catctccggc ggatagcgtt cccaatgagt ataattatct
     4681 attatataaa caatgtcccc acaaaggttc atcaagaatg ttcttcttcg tccttaagct
     4741 gaaaaataat gtcctccttg tccgtatcaa gggtgagact ttttccctct aagtgccact
     4801 gcggatatta actgccgctt tctactcccc tgatgtcatc tattctccac tcctaaatcc
     4861 aagtatagga aaaaataaat ctatgtttct atgcttgaca atatcccatt ttttactata
     4921 ttatgaatca aataatttta tttcgaaaat ttatatgtta ggtgtttgcc atgggagaag
     4981 tgttagattt agtttgaaca atgaatatta gttggcaaag cgctagcaga gtaattgacg
     5041 ctgtcaatgc tggtgcatcc atttggggaa ttgtctctat tattgtagca ggtggtggaa
     5101 tcctcggtat gggatatgcg gctttggccc ttcttgtaaa gaaaaaagta aaagatttag
     5161 gggaaacagc agcaatagct tggtaattgt ttgatttatg aatcggtttc ggataaggcg
     5221 tgtctcttga cacgtcatta tccgatatta ctaagaatct cgtgactatt agtcataaga
     5281 ggttcaatat gagacaaagc agctttaagg atgaaattaa ctggagggtt tatcatttct
     5341 aaccttgtac gcattgttta ctatcagcat atgcttcatt accggtggtt actggatcac
     5401 tttatagcac tgactgattc ccgacttgtt tatgctaagc ttttgttgtt tttgtttcct
     5461 tttacactag gggccttttc cttctatata tccaaaggta ttttttccta caccctgtct
     5521 ctggaatggg tccctgcccg gagtactttg gaagcgttgt ttatgatcgt tttctttctt
     5581 ttattttggg tgttgttgtt tcgtgactat aaacataatt atgcaagagc cttttactca
     5641 catgatagta ccctatactt ggcacaagga attcatttta ttctaatact tactgccaag
     5701 ttaatagatg tcttctttct aagtgtatgg ttattcattt atcccatcct gatcggaatt
     5761 tcattggcct ggaattccgt ttattcaatc aagattcctc tacagtggtt cacgattgat
     5821 ttcgggttac tacttgttct gatcttattg ctgaggaacc tcagtatgat tatcatgttt
     5881 ctcgtgaaat caaccaaaaa acaaaggggg cagacctatt ggtttcagca tctacacgta
     5941 ctagctttag gtaaatatat tttcattggt ataggcagct acatagtcac aattcttttt
     6001 atccaaaccc atacacctgc ttggtcttgg cagtctctgc aaagtgagca cttgaacctg
     6061 ttccataagt tgtcctttcc tactcagtgg ctaattacag ggaaggaatt ttatttcctg
     6121 ctcttgcttt ttagtttact gatcactttt gttacacttg taatcgggtt taaccggatc
     6181 ctgtatgtaa aatcccagct tacgacaaga tatgtgaatc gtgattattc gagtggttta
     6241 aggcttcttg aaacccccaa tatgcccaat tggctgctat taataatgaa agatctgatc
     6301 atcgtttggc gggataagcg aacacttatg actccagttg gacagttctt gtctatgttt
     6361 atggcgtttt tgggaacttt gggggcactt cgaaattcag aattatttca ttcccagact
     6421 gtatccttat ccgccattat tgcgatgcaa tttatttttt ctacatttgc cggaaacatg
     6481 atttcccgga taacaagtat agatgctgaa ggccgtatga tctctactct attgctttat
     6541 ttacgttccc cggctgtttt catgaaatca aaattgattc tgcatagtct atttgtaggg
     6601 ggagttataa cctctgtaac aatcttactg tccttgatgt ttcgtataga agtaactttg
     6661 tttctttatt cactttgcag cagtttattg attaccatga cccattcttt aagttttatt
     6721 gttagcagcg ccatttttcc taaatttaat tgggatcatg aaacgagaat aggtacatcc
     6781 tctaaagctg ttctattgga gtccctgttt ttgcgtgtgt atgaatttgg atgtatgatt
     6841 tcaattgcgg tagccgctgt ttacctgtat acaaatgcac taagcccaaa tcaatattat
     6901 atctgcatct tatgtatcat tattttgttt agctctgtat ggatatccat acttttattg
     6961 ttgtatcgaa taccttggtg gaaaggatgg aagttttgaa tccaattgaa gtttacggaa
     7021 tatcttatca atatgtgggt acaaaagatc tggctgtgaa agatataact tttcacgtaa
     7081 aatcaaatga aattgtgggt ctcttgggtc acaacggatc gggaaaaacg accatcatga
     7141 attgcatcgc aggcttattt cagcctgtac aagggaaagt tttattatcg ggaacccaaa
     7201 tttcggatct gtgtaagccg aagccagtcg gttatgttcc tgcggatccg atcctgtaca
     7261 acatgttgac cgtacaagaa tacattgtgt ttattggtaa attacaccac ttatctacgg
     7321 cacagatcca tactatttcc gaacctttac ttgacatgct gaaactaaaa gacaaccgcc
     7381 ataagcttat taagcactta tctcatggaa tgaaacaaaa agtatccatg ataaccggac
     7441 tgcttcatca accgcccgct cttgttttag acgaacccat gactgggttt gatgcccaat
     7501 caaccaagga aatgaaagat ttcttggttc atttggttcg aaccaatcat ggagcaggga
     7561 tattattatc ctctcatcgg ctcgatatcg tggagagcat ctgtgatcga attgtggtta
     7621 ttcgtcaagg gcagttgatc ttcactggct ctgttcaaga attaaaacat agtgcccaac
     7681 atgggaattc cctggaagag gcatttttga gtattacttc tgagaatggg gggaactaaa
     7741 tgagaggcaa acaggtagta aaccaaatct cattgatttt tattttcgct tttatcggta
     7801 tttatggtga tatccagtgg tatcagaaag ggaatgatgc ctcccttttt attatgggaa
     7861 tggcactgtt tggaacgtct tacgccatcg tattaattgt tgatcctatt gtaggttggt
     7921 taaaaaagaa atgatccgcc aagtagaaat aaagtacatt cgaatcttgc tccttatttc
     7981 atgtatgatt tattttttat caacatgtat tggttttttt tcaggaccct acttacagtt
     8041 aaatccgatt gctctctccc tttccacaaa tccctatact tatatcatcc ataatttcaa
     8101 aagcatggtt ttttttataa gcggattgtt tacgatgggg atttccacga tgtatgttct
     8161 ttttatgaat gggtttgtgc tgggggcctc tctttatgga attggcacgg cctctacctt
     8221 tttcagtgct ttcattagaa ttataccgca tggaatattc gaacaagaag aaggggtgta
     8281 tttaccctag gttttatttg gttttgtacc accatctcta tcccggctct tcaacttttg
     8341 ttagtttagt ccaaactgct ctgtaagtaa agatccccta taagtagaca gctcaccatg
     8401 tctgcttata agggacagtt ttaggcatct ggaccgctgg aggttgtggt tgcggatatg
     8461 gaagagcgtt tttttaatcc tcggtcataa ataccggcaa taaggattga aacgaacaaa
     8521 agtgcgatga gcaggataaa taaaacatag atgttataaa aatccaccag aattccgccc
     8581 agaagcggtc ccagcatttt accgatggtt cctgcgctgt tcaccactcc ctggtacatt
     8641 cccagtctat tctccggggc cagtttattt gcaatggttg gaatggcagg ccacacaagc
     8701 atttcaccta aggaaataat aatcatggcc acgaggaata ttttgaattc cctggcaacc
     8761 agcaagattc caaaggaagc aataaaaacc agaataccta aaataatttg ttttttaagg
     8821 gatgagatta ttttgggtat gagtgcggtc atgaccggct ggccgaatac aatcagaatc
     8881 ccgttgattg tccaaagcag gctgtatttt ttcaggtcga tggaaaggct ttgcatatgt
     8941 accgaaatcg ttgcctgcca ctgtgtgtag gctagccagc acaaaatata agccccgcag
     9001 ataatcaaca gagctttaaa ggacgaggtg agctttgttt ttatcaaacc tcttttttct
     9061 ttgccgacgg gacctgtgga cgttggaaaa cgtttgaatg taaacaccac cagcaaagta
     9121 aataaggcat agacagcggc atttccccag aatatatagt cgaatgaata tgaagcgata
     9181 tatccccgag tgcagagcca agcgctacac cggcattttg ggcaacatag atcgcggcga
     9241 acgtcttgct tcctccactg ggccaaatca acccggctga agcatatatg gaaggaacca
     9301 ccatgccgga acccaatccc attacaatca aacagactac ataaaaccac cagtcgtgat
     9361 taaaagccaa taccgtggcg gccgcaagag tcaggacaac gccctggacg actgtccggt
     9421 aactgccgat cctgtcaaat aaataccctc caagcaggct tccgacgaca gaagcaaatg
     9481 aattcaaagt caggacaaat ccggcaacgg ccaatgcctt ccccaaatga tagtggatat
     9541 aaatagtatt gagcggccaa ataaaagatg cgcccgtaac attgataaac attcccatta
     9601 ctaacaggca taacttttta ggcataaaac cacctcctgt gtgattttat cctctcatct
     9661 ctttccattc ggaacgcact ccctccatta tatatagccc ccgtatgaaa aagaaagacc
     9721 ttttttcagt ttcattttac taaaaaagaa aaagtcacgc ccacgatcag cgcgacttca
     9781 tatttcatat gtttcattaa cgaaagtttt ggggctaagc atttttttcg aattgtgcca
     9841 tccgctctat cagctgtccg gaagtaatat gcggattacc ggcatttatg cggtaaattt
     9901 ctttttgatc tgcatgattg atgccttcct ctgtcagaaa gaacagtttg atcccaagtt
     9961 cttgtccaac ggttagcacc tgttcattta tatatccgta aggtaacgcc aggaggctga
    10021 tttgctctcc cagttcctct ttcaatcgtt tttccataaa tgacaagtct gtcttgaccc
    10081 gccgaatata ttcgttcgcg tcttcactac gctgcttatt cggcatatat ttggcaatcg
    10141 taagcgcagg cttcgggtct ccttccttgg aggtgttatg cttgtg
//
