LOCUS       NZ_CP020557            10195 bp    DNA     circular CON 24-JUL-2024
DEFINITION  Paenibacillus larvae subsp. pulvifaciens strain SAG 10367
            chromosome, complete genome.
ACCESSION   NZ_CP020557
VERSION     NZ_CP020557.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus larvae subsp. pulvifaciens
  ORGANISM  Paenibacillus larvae subsp. pulvifaciens
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 4666454)
  AUTHORS   Dingman,D.W.
  TITLE     Paenibacillus larvae genome sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4666454)
  AUTHORS   Dingman,D.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2017) Entomology, CT Agric Expt Station, 123
            Huntington St, New Haven, CT 06504, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2016
            Assembly Method :: SMRT Pipe HGAP assembly v. February-2016
            Assembly Name                     :: sag10367-hgap-022916
            Genome Coverage                   :: 214.2x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002082155.1-RS_2024_07_24
            Annotation Date                   :: 07/24/2024 02:27:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,275
            CDSs (total)                      :: 5,166
            Genes (coding)                    :: 4,634
            CDSs (with protein)               :: 4,634
            Genes (RNA)                       :: 109
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 532
            CDSs (without protein)            :: 532
            Pseudo Genes (ambiguous residues) :: 0 of 532
            Pseudo Genes (frameshifted)       :: 400 of 532
            Pseudo Genes (incomplete)         :: 184 of 532
            Pseudo Genes (internal stop)      :: 26 of 532
            Pseudo Genes (multiple problems)  :: 72 of 532
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:07:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 46163
            Orig. end                         :: 56358
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020557.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Dr. Adriana Alippi,
            CIDEFI-CIC, Facultad de Ciencias Agrarias y Forestales, Universidad
            Nacional de La Plata, 1900 La Plata, Argentina.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10195
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10195
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10195
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5195](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5195)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    240..255
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 20.69"
                     /tool="antismash"
     misc_feature    complement(291..310)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 20.91"
                     /tool="antismash"
     misc_feature    689..704
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 24.12"
                     /tool="antismash"
     misc_feature    2311..2325
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.5"
                     /tool="antismash"
     misc_feature    2802..2818
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 22.33"
                     /tool="antismash"
     repeat_region   2810..4407
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=49043..49074
                     /rpt_unit_seq="atttcaatccacgcatccgcgtaggatgcgac"
     misc_feature    2862..2876
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.83"
                     /tool="antismash"
     misc_feature    complement(4798..4811)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.69"
                     /tool="antismash"
     misc_feature    complement(4799..4812)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.91"
                     /tool="antismash"
     gene            complement(5001..5195)
                     /locus_tag="B7C51_RS00325"
                     /old_locus_tag="B7C51_00325"
     CDS             complement(5001..5195)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B7C51_RS00325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B7C51_00325"
                     /product="hypothetical protein"
                     /protein_id="WP_042118951.1"
                     /sec_met_domain="TIGR03651 (E-value: 0.0003, bitscore:
                     19.2, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFELIASGISAGNAAQVINAVFNGLSIVSALSLCAGIAGGAAWFL
                     SNGIGYMIKWAGKKTIIDW"
     gene            complement(5224..5997)
                     /locus_tag="B7C51_RS00330"
                     /old_locus_tag="B7C51_00330"
     CDS             complement(5224..5997)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /locus_tag="B7C51_RS00330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00330"
                     /product="stage II sporulation protein M"
                     /protein_id="WP_083038043.1"
                     /transl_table=11
                     /translation="MKAYISVGHKAAIFLIGLSILVGFIYGFVFEDIDQIKTIGFDGLN
                     VLETFFQIFFHNFIIGLIIIFCSIFFYIVSAIPVIATYFVFGESFAIILKEKGLIAAIS
                     TYPHFLPETLSTMLLLSIAFVISAKMLKIVFKNESILKNKKKILKTYLVSNVLLIFAAF
                     IEALKIGDRKLINEIYFVSIGSKLIFSTVAVLTLISATSWNFIFASVGAIILYIIYFFA
                     NKQNTISERTRKVIARGDECLRIYSYGSRGFMPCL"
     gene            complement(6374..7171)
                     /locus_tag="B7C51_RS00335"
                     /old_locus_tag="B7C51_00335"
     CDS             complement(6374..7171)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /locus_tag="B7C51_RS00335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00335"
                     /product="phosphotransferase"
                     /protein_id="WP_083038045.1"
                     /sec_met_domain="APH (E-value: 3.1e-12, bitscore: 45.1,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHSLHPPAPGSRGLNRPVGNIRAFRLSTDKGDFLVKPFSRRKTGP
                     KLTTREQITRLSSYIQKLKESGYPHLPNWLVTKSGQYWVSHNGRPYYMTDWVEGSGIQS
                     EEDYENLGRALATLHNNCKDSLPSMSRYTYKQTKLFKLQDQLFRLQLLTIRRNKSKGKW
                     FREHGGLCIELANKSWSILRKPETKQIMEEEINHPALIHGDVTLPNIIIHPSRPFLIDW
                     DLLRMGSTYYETAKTLLNTTNFDPANISALIKGYEQIKGLTPA"
     gene            <7109..7309
                     /locus_tag="B7C51_RS00340"
                     /old_locus_tag="B7C51_00340"
                     /pseudo=""
     CDS             <7109..7309
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010495662.1"
                     /locus_tag="B7C51_RS00340"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00340"
                     /product="fatty acid hydroxylase"
                     /pseudo=""
                     /transl_table=11
                     /translation="YVAHRPIQPATPWGRWMKRMHLWHHFKNEQYWYGVTNPVFDLTLH
                     TYKDGKEVEKSGTTRNMEKLE"
     gene            complement(7558..8000)
                     /gene="cynS"
                     /locus_tag="B7C51_RS28625"
                     /old_locus_tag="B7C51_00345"
                     /pseudo=""
     CDS             complement(7558..8000)
                     /GO_function="GO:0008824 - cyanate hydratase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="cynS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081569.1"
                     /locus_tag="B7C51_RS28625"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00345"
                     /product="cyanase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MERELAGKIIVEAKRKLGLKWADIAKVIDRSEAWTASALLGQATL
                     SNEEAEQVASLLELDQGLPSA"
     gene            complement(8136..8558)
                     /locus_tag="B7C51_RS00350"
                     /old_locus_tag="B7C51_00350"
     CDS             complement(8136..8558)
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019909483.1"
                     /locus_tag="B7C51_RS00350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00350"
                     /product="OsmC family protein"
                     /protein_id="WP_083038047.1"
                     /transl_table=11
                     /translation="MKVTTTWQGKRAFTSVGPSGYSVTMDATAAYGGDGKGATPMELIL
                     AGLAGCMGIDITMILRSTLDTIQSIEIEAEGTRDEKPPKGFTAIDLVFKINGDVPDHRI
                     WRAIELGKEKYCAVSDSLKADIRYKLILNGKEAVKP"
     gene            complement(8603..9349)
                     /locus_tag="B7C51_RS00355"
                     /old_locus_tag="B7C51_00355"
     CDS             complement(8603..9349)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018756024.1"
                     /locus_tag="B7C51_RS00355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00355"
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="WP_083038049.1"
                     /transl_table=11
                     /translation="MIQTTGLTKSFQDTVVLKDINLQVKQNEIVVLLGPSGSGKSTLLR
                     CINGLEELSGGTVEVNGIHIDAKMPARQRRARILEIRRETGMVFQQFNLYPHKTALGNV
                     IESLLVVKKMRREEAIKIGEQLLDRVGLADKKDVYPSRLSGGQQQRVAIARALAMEPKI
                     MLFDEPTSALDPELVGEVLDVMQQLAKDGMTMLVVTHEMKFARKVADRIVFMDAGIIVE
                     EADPDAFFESPQSERARKFLKILDHE"
     gene            complement(9371..10015)
                     /locus_tag="B7C51_RS00360"
                     /old_locus_tag="B7C51_00360"
     CDS             complement(9371..10015)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018756025.1"
                     /locus_tag="B7C51_RS00360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B7C51_00360"
                     /product="amino acid ABC transporter permease"
                     /protein_id="WP_083038051.1"
                     /transl_table=11
                     /translation="MSLVLENIPFLLKGAYYTLLITIVSMFFGLIIGVVVAVARIKGNR
                     PIQWLARAYVSIIRGTPALVQIFIVYYGLVDYGIALSSLTAAFVALSINVGAYLSETFR
                     GAILSIPKGQTEAAYAAGMTSWQAMRRIILPQAARVAIPPMGNTFIGMLKETSLVSSIA
                     VTELLRSAQLMIAQYYVNMPFYIGIAVMYWVMSTVFSSILGAIEKRLAKAY"
     misc_feature    complement(10080..10094)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.72"
                     /tool="antismash"
ORIGIN
        1 taaagaattc ttctttcgtc ttgggacggg tatacggcag gacgggacga atctcttgtt
       61 cctgcagcat tttgcagatg gctggtgttt tataacctgc atccgcggca accgcttgtg
      121 gagccggaac tacgcgagta acctcgtgta gaacatcttc aaatacttgg ctgtcgtgga
      181 tatttccagg ggttactttg acgccgagta caaacccgtt ccgatcacac gcggtatgga
      241 agctgtaggc gaaaacacgt tctttctctc ccttcacgaa caaaccacac tccgggtcag
      301 tcgtgctcac cttcacctcc ttctgcgtga cattgggctt tttttcgaac ggtttctttc
      361 catgtagagc ccgatcttga ttgatctctt catcaagctg atcctggtac tttcgacttt
      421 gctcctgaac gatctgtttt acatatttgt ttttgttcgc gcttgcttta acatgtgtcg
      481 cgtcgatgaa gagtacgtca ggttcaatga aaccatgccg ggcagcttct tcgaggatgc
      541 gatgaaagat tccctcaaac agtccagtat cccgaaaacg gcgcacgtaa tttttaccca
      601 gcgtggagaa atgaggaatg ggctgactga agtcatagcc aataaaccaa cggtacgcga
      661 cgttggtttc aatctctttg atggtctgac gcatggagcg aacaccaaaa aggcattgaa
      721 taagcgcaat tttaataaga acaaccggat caatgctagg gcggccgtta tcctcacagt
      781 aaacatcccg aaccaagtca tagatgaaac taaaatcgat ggcgtattcg attttacgga
      841 ccaaatgatc tataggtaca agctcgtcca aggagaccat ggaaatttga gaacgtcctt
      901 cgtaaccggt ttttcgcaac atattcaagc actcccgcac tagttattta cctatatgat
      961 actatatgaa tgcggtgaat ggttggtgta ttttcgtaaa aaagactgtc gacaggactt
     1021 tgtcgacagt ctgagaaggg gagtttcccc ttctatttaa taaaagttga gaattaccaa
     1081 gcaatagtag ctctttttcc tttttcttga attttctttt tcaagaacta gcgtattgtt
     1141 tctttacctg cagcagcaat taaagaaaga ccgcctgaac caacggctaa caaaatacta
     1201 acgattgttg tgacagcacg tcctgcattc acaatattta gttacaactg aagcgatact
     1261 agctgggatc cgaattcttt tgctaaatct gcgactggaa gcagttgaag agagaacaaa
     1321 aagagagcca atatcaaaaa tcctaccgct aaagccgaaa agatgttttc atttcttgtt
     1381 tctaacataa acaattaatc ctttaaatgg tttttatgac atttatagtc tatcattcgt
     1441 ttgaaccatt attaatacac attttaacat aaataactct atttaacatc ccttatgtag
     1501 gggtgatgga tagaagcaga ctagattaga acatccttaa tttaccatat aaatacataa
     1561 attttacaat atacatttta tgtatttatt ctcatttgaa tttaaaaggg agcataaata
     1621 taacaaatat ggtactaagg aatgtaagag gaagattgaa atgtccgacg atcaatggtg
     1681 gattcccgca gatctttcca taaaacgaag ggataattcc tgaacaatca ggtgatgggt
     1741 tccccatctt ccgaggttaa aagagaaatc tgacttggta aacattacat aaactcatga
     1801 tgatccgacc ggcaatactt tcagatacct ctcctcacat tcagcctgaa cgcccaccga
     1861 agtaacatag tagtcacttc ctgatacata taatgcttgg caagccctgg tacaaataac
     1921 tgtaattgag agtcagagcc agatacgata tcatccacaa aatggcttga aactatttta
     1981 tactgtgtta aagaggctta tttccgggtt gccaaccaaa cattataaac tcagctaagg
     2041 aattttgtga tcctacgaaa ttaccggctt ctgttcttgg atgcggagtg gttttaacgc
     2101 atttggtgca gtatcttttt taatgatgag gatgtttcca tgtacccgta catgctccat
     2161 attataatca attggaacac ggctggtact tagatagttt gtggttagat cgtaacaatt
     2221 cagaaggaat catattcatc ttctcaatcg gttcacttct gcgacataag ctgttgactg
     2281 ttcggtaaca tattgtcatg taataatagt ttaatttcat ttccagtgac tctctctttc
     2341 ggtgtatttc tcttcttttt ttatgcttat gcagcatata tatcctttat tgttacatat
     2401 atctaacttt ttcaatacta cttaggtatt ataatgagta acgaactcct tcgaactatt
     2461 ggaaaaaggg ctgtccaatg aacagcccct gtcaagtaga cagtataaat aataaaaaaa
     2521 cgttaaacgg acttggtcct ggactccatt ggactaagac taatttggtt tgtagtcact
     2581 cgtaattgta aaattggaat ttttgcgatt tcacacaata actgaatgct gaatgactct
     2641 tcctgctgaa ccccttgaat ggttaggtag atattctcct gtcggtgctg gcttaatgtt
     2701 tccccttttt ttaacctgtc taccttaagg gggaaatacc ataaaattag atgccttttg
     2761 gcgttgtccc gtccgattgc ttgaaacgcc atagttggta gtgattaaaa agtaggcctt
     2821 tgcatccgcg taggatgcga cgcgacgctc ccggaatacc tcgtttagat cggccagtga
     2881 tttcaatcca cgcatccgcg taggatgcga cacgccttac gcctcctttt tcttgcggat
     2941 gacaaaattt caatccacgt atccgcgtag gatgcgacag taaagcaaac cctacgatcc
     3001 cagcttgctt ttttgtattt caatccacgc atccgcgtag gatgcgacca actcttaaag
     3061 ctcgccactc tcttgagtta taatttcaat ccacgcatcc gcataggatg cgacgctccc
     3121 aacacctacg agcaacgctt ggcgtttgta aaatttcaat ccacgcatcc gcgtaggatg
     3181 cgacaactga aactaagtct ttaactatgg ctgatattat ttcaatccac gcatccgcgt
     3241 aggatgcgac gcgtgcggac ggccgccaca taatcggggt ttttatttca atccacgcat
     3301 ccgcgtagga tgcgacattc gcctgtcgcc atatgacact ggccacagaa cgatttcaat
     3361 ccacgcatcc gcgtaggatg cgaccatatc gtgagtcagg cgacttacga ttcataccgt
     3421 atttcaatcc acgcatccgc gtaggatgcg actgtaaccg tagatacagt tcataaaatt
     3481 aatgagacta tttcaatcca cgtatccgcg taggatgcga cacccttcgg cttctgcaac
     3541 agcagatacc cggtcataat ttcaatccac gcatccgcgt aggatgcgac caaatgtttt
     3601 cccttcagta taggctcccc tcgatttcaa tccacgcatc cgcgtaggat gcgacggaaa
     3661 ctggcgagcg catcgtatgg gggactgcat cgatttcaat ccacgcatcc gcgtaggatg
     3721 cgacaacgca acaagtttcc ggtttgcagt taccaaatcc ggatttcaat ccacgcatcc
     3781 gcgtaggatg cgacaggtta tctccaccta gtccactcta cttggtcaaa tttcaatcca
     3841 cgcatccgcg taggatgcga cattcgcctg tcgccatatg acactggcca cagaacgatt
     3901 tcaatccacg catccgcgta ggatgcgacc atatcgtgag tcaggcgact tacgattcat
     3961 accgtatttc aatccacgca tccgcgtagg atgcgactgt aaccgtagat acagttcata
     4021 aaattaatga gactatttca atccacgtat ccgcgtagga tgcgacaccc ttcggcttct
     4081 gcaacagcag atacccggtc ataatttcaa tccacgcatc cgcgtaggat gcgaccaaat
     4141 gttttccctt cagtataggc tccccctcga tttcaatcca cgcatccgcg taggatgcga
     4201 cggaaactgg cgagcgcatc gtatggggga ctgcatcgat ttcaatccac gcatccgcgt
     4261 aggatgcgac aacgcaacaa gtttccggtt tgcagttacc aaatccggat ttcaatccac
     4321 gcatccgcgt aggatgcgac aggttatctc cacctagtcc actctacttg gtcaaatttc
     4381 aatccacgca tccgcgtagg atgcgacatt gactcgtatt gacgcacgtc gcgctctgga
     4441 aaaagcccat ccaagcctga gaaataaaaa gcaacaggag aatagctaaa accttaattt
     4501 ctaataatat gactaagttg attagaatca ggatgattcc atttaactca aataacgcat
     4561 ctttaataaa cactttatgc atccatgttc taataggcag gattttgaat tccttcactt
     4621 tataaaggta ggagatgaat aaaaggagaa gaagaacata tttcaagaat agtggatacc
     4681 aaaaaatggt taaagggaac gcgcatttaa aaaaagtttc tgacaaagtg aagcatgtgc
     4741 ttaacattct gcaaatgtta gtaggtaaat aagtaaattc gtcactgaaa aatctcattt
     4801 tttttgaaaa atgaagtcga taatgctttt ttataaaaag aactaggtat tgcaatatat
     4861 aaaaaagctt tttgggaaca ttcataagac ctccaaaacc tctgaatcca tatatgcatt
     4921 gaaattgatg aataaataaa ataaaacaag ggatttaagg gattatcatt gagtttggta
     4981 atcccttgtt ttatggttaa ttaccagtca atgattgtct ttttaccagc ccattttatc
     5041 atgtacccta ttccattaga taaaaaccat gctgcaccac ctgcaattcc agcacaaaga
     5101 gataaggccg acacgataga taaaccgtta aaaactgcat taattacttg agcagcattt
     5161 cctgcagaaa taccacttgc aattaattca aacatattat caccccctcg aagttaatat
     5221 gatttataga caaggcataa agcctcggct accataagaa taaattcgga gacattcatc
     5281 acctcttgcg attacttttc tagttcgttc tgagatagtg ttttgtttat tagcaaaaaa
     5341 ataaataatg tataagataa ttgcacctac acttgcaaat ataaaattcc aagatgttgc
     5401 tgaaattaaa gtaagaactg ctactgtcga gaaaatcagc tttgacccaa tagagacaaa
     5461 atatatttca tttatcaatt ttctatctcc aattttcaaa gcttcaatga aagcagcaaa
     5521 aatcaacaac acattagaaa caagataggt ttttaaaatc ttttttttgt tctttagaat
     5581 ggattcattt ttaaatacaa tttttaacat ttttgcagaa ataacaaatg ctattgagag
     5641 taataacatg gtggaaagtg tttccgggag gaaatgaggg tatgtactta ttgctgcaat
     5701 aagtcctttt tcctttaaaa taattgcaaa actttctcca aaaacaaaat acgtagctat
     5761 tacaggaatt gcagatacaa tatagaagaa gatagagcaa aatataatga ttaaaccaat
     5821 gataaaatta tggaaaaaaa tttggaaaaa agtttcaagg acattcaatc catcaaaacc
     5881 tattgttttg atttgatcta tatcttcgaa aacaaaacca taaataaaac ctactaaaat
     5941 acttagacca attaagaaga tggctgcttt atgtccaaca gaaatatagg ctttcatatt
     6001 cacttcacca aaacctttta aaagataata gaaactctac ttatttttaa caccccctaa
     6061 tcttgaaaag agggatgatg gttaaaagca aactagatta gcatatactt aatttaccat
     6121 ataaatacat aaaatgtaca atatgaattt tatgtattta ttctcatttg aatttaaaag
     6181 ggagtatgaa tataacaaaa tatggtatta attattgccg tgcccattca tccacccatc
     6241 gtatggcacc aagtcttttc tcccatgttc ggctcaaaat atgaaaaacc ggcgaacgcc
     6301 tgcctgacgc aatcctccgg gctgatctcc atgcctcgac aggaaggcga aaaaaagcgc
     6361 ttatgagcaa tcgttaagcc ggagtcaacc cttttatttg ttcgtaaccc tttattagag
     6421 cagagatatt tgccggatca aaattagttg tatttagcaa ggttttggcg gtttcgtagt
     6481 aggttgaacc cattcttagc agatcccagt caatcaggaa tggtctgctt ggatgaataa
     6541 tgatgttagg caaggtgaca tccccatgaa ttaatgcggg atgattgatt tcctcttcca
     6601 tgatctgttt cgtttccggt ttccttagga tgctccatga tttgttggcc agttcaatgc
     6661 aaagtccccc atgctctctg aaccatttcc ccttgctttt attccgccgg atagttaata
     6721 actggaggcg aaagagctga tcttgcagtt taaacaactt ggtttgctta taggtatacc
     6781 gggacatgga aggaagagag tccttgcaat tgttgtgaag agtcgccaag gccctgccga
     6841 gattttcgta atcctcctcg ctttggatgc cgctgccttc aacccagtcc gtcatatagt
     6901 agggcctgcc attatgagag acccaatatt ggcctgattt tgtaaccagc caattgggca
     6961 agtgaggata tccgctttcc tttaattttt ggatataaga agagagacgg gtaatttgct
     7021 ctcttgttgt caattttggc cctgtctttc ttcgggaaaa gggctttacc aaaaaatccc
     7081 ctttgtccgt tgacagacgg aaggccctta tgttgcccac cggccgattc aacccgcgac
     7141 tccctggggc aggtggatga agagaatgca cttatggcat cattttaaaa atgagcaata
     7201 ttggtatggt gtaactaatc ctgtatttga tttgactctc catacgtaca aagacgggaa
     7261 agaggtggag aaaagcggga caacgcggaa tatggaaaaa ttggaatgag cgcgggatct
     7321 gggggcaaga gctctggtcc tggagaccca aacgtccaat tttccggcta ttagcatccg
     7381 ttaataataa ggagaggctt aatagcctct ggagcttgtc aagaaaccca cttcacatag
     7441 aaattgtctg tatagtccgg tgggggatcc ctccaagccg ctgcccggaa cccgtgaaag
     7501 aaatgaaagc ctgaagaggc ttaaacagcc tcttcccgct tatcatagca catgccgcta
     7561 aaactttttg taagggagga attttccgtt tagcatcatc agtacgcggt cacctttcgg
     7621 atccgggacc cgtttaatat ccatctcgaa atcaattgca gacataatgc catcgccaaa
     7681 tttttcatga ataatttctt tcattccggg accgtaaact aagagtattt cataaaaacg
     7741 ataaagaagc ggatctgtag gcggcatctg aatagccgtc ccccggtaag gtacctctgt
     7801 taagcactcg gcaacccctg atcaagctcc agaagagaag ctacttgttc tgcttcttca
     7861 ttggaaagtg tagcttgtcc taataatgcc gaggccgtcc atgcctccga gcggtcaatg
     7921 actttcgcga tatccgccca ctttaatcct aatttcctct tggcctcgac tatgattttt
     7981 ccggcaagtt ctctttccat tttgtttctc ctctcttgtt tatactttcc cttcatttta
     8041 ttcggcctta taaatctcca ttccccggtt tgctaaactg ttagacaaaa aaaaagacgg
     8101 gccccggcga tccggttgac ccgacttttg aaaaattaag gcttcactgc ttctttacca
     8161 ttcaaaatga gcttgtaacg gatatctgct ttgagagagt ccgaaacagc acagtatttt
     8221 tcttttccca attcaatggc tctccagata cgatgatcgg gaacatcccc attgatttta
     8281 aaaacaagat ctatagccgt aaacccttta ggcggtttct catcacgggt tccttccgct
     8341 tcaatctcaa tactttgaat agtatccagc gtcgaacgta aaatcatggt gatgtcgatc
     8401 cccatgcagc cggcaagacc tgcaagaatc agttccatag gagtggcccc tttgccgtct
     8461 ccgccgtaag cagccgttgc gtccatagtg acgctgtatc cggaaggccc aaccgaagta
     8521 aaggcccgtt ttccttgcca ggttgtcgtt actttcatat ttgatttccc ccttttctcc
     8581 tgtgaggtaa gagtcattat ggttattcat gatccagtat tttcagaaat ttccttgctc
     8641 tttcgctttg cggactttca aagaacgcgt ctggatcggc ttcctctaca ataattcctg
     8701 catccataaa gactatgcgg tccgccactt tcctggcaaa cttcatttcg tgtgttacaa
     8761 caagcatggt catgccgtct tttgccaatt gctgcatgac atccagtacc tcacccacaa
     8821 gttcggggtc aagtgcggag gttggctcat caaagagcat gattttgggt tccattgcca
     8881 gcgcacgtgc tatagcgacc cgctgctgct gacctccgga aaggcgggaa ggatacacat
     8941 ccttcttgtc agcaagtccc acccggtcca acaattgttc gccgattttt atagcttcct
     9001 ctctccgcat cttttttacc accaacaatg attcgataac gttccctaat gcagtcttat
     9061 gcggatataa attaaattgc tgaaatacca tcccggtttc ccggcggatc tccagaatgc
     9121 gtgccctccg ctgacgggcc ggcattttag cgtctatatg aatgccgttg acttcaacag
     9181 taccgccgct caactcttca agtccgttga tgcagcgtag cagcgtactt ttccctgaac
     9241 cgctcggacc gagcagaacg acaatttcat tctgtttgac ttgcaaattg atgtctttca
     9301 acacaactgt gtcctgaaag gatttggtta atccggttgt ttgaatcatg ggtcttctca
     9361 ctccttgccc tcaatatgct ttggctaacc gcttttcaat cgctcccaat atggaggaga
     9421 aaaccgtact catcacccaa tacataacag caattccgat atagaaaggc atatttacat
     9481 aatactgggc aatcataagc tgtgccgatc gaagcagctc ggtaactgca atggaggaaa
     9541 cgagtgaggt ttctttcagc atgccaataa aggtattccc cattggcgga attgccactc
     9601 gagccgcctg gggcagtata atcctgcgca tcgcctgcca ggaagtcatt ccggctgcat
     9661 aggctgcctc tgtctgccct ttcggaatgg aaagaatggc tccccggaag gtttcggaca
     9721 ggtaagcacc gacattgata ctaagtgcaa caaaagccgc agttaatgag cttaaggcaa
     9781 ttccgtaatc aaccagtccg taatagacaa taaaaatctg aacaagagca ggcgtgcccc
     9841 gtataataga tacgtaagca cgcgcgagcc attgaatggg acggtttccc tttattctgg
     9901 caacggcaac cactactcca atgatcagac cgaaaaacat ggacacaata gtgattaata
     9961 gtgtatagta agcacctttc aataaaaaag gtatattttc aagaacaaga ctcataacct
    10021 gccgaattcc cttctcccta tcctggtatc attattcagc cggaggttct tcaccaaacc
    10081 attttgtgaa aatagtttta taagtcccgt cagctttcaa atcttttagc gcctgattca
    10141 gggcatcccg cagctcagta ttccctttgg tcactgcaat accggcctta tctga
//
