LOCUS       NZ_CP020555            10960 bp    DNA     linear   CON 05-MAR-2024
DEFINITION  Streptomyces sp. Sge12 chromosome, complete genome.
ACCESSION   NZ_CP020555
VERSION     NZ_CP020555.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. Sge12
  ORGANISM  Streptomyces sp. Sge12
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7983613)
  AUTHORS   Tao,M., Xu,J., Xu,M., Liu,K., Zhao,Z. and Peng,Q.
  TITLE     Genome sequence of Streptomyces sp. Sge12, producing unknown
            compounds against rice blast disease
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7983613)
  AUTHORS   Tao,M., Xu,J., Xu,M., Liu,K., Zhao,Z. and Peng,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, Huashan Road 1954, Shanghai 200030,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: RS_HGAP Assembly v. 3; BWA v. 0.7.10; Bowtie v. 2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 85.0x
            Sequencing Technology :: Illumina HiSeq 4000; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:12:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,312
            CDSs (total)                      :: 7,216
            Genes (coding)                    :: 7,106
            CDSs (with protein)               :: 7,106
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 32 of 111
            Pseudo Genes (incomplete)         :: 91 of 111
            Pseudo Genes (internal stop)      :: 8 of 111
            Pseudo Genes (multiple problems)  :: 18 of 111
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:35:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7972136
            Orig. end                         :: 7983096
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP020555.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10960
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="41"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10960
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10960
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5960](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5960)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            404..733
                     /locus_tag="B6R96_RS38390"
     CDS             404..733
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /locus_tag="B6R96_RS38390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_237291730.1"
                     /transl_table=11
                     /translation="MGWGVGVRVLVHRAGVSRVGVEVVRGDLVRPGSLRGVCEGVSSVL
                     HLASRIGGSVGELEAVNVEGTRALLGEAARAGVERVVQLGTAGVYGDGVHRGWWRGSWW
                     SVRGR"
     gene            complement(1621..2544)
                     /locus_tag="B6R96_RS35895"
                     /old_locus_tag="B6R96_35895"
     CDS             complement(1621..2544)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (22-189). E-value: 1.7e-29.
                     Score: 94.9. Matches aSDomain:
                     nrpspksdomains_B6R96_RS35895_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020124491.1"
                     /locus_tag="B6R96_RS35895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_35895"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_107475436.1"
                     /sec_met_domain="ADH_N (E-value: 2.2e-08, bitscore: 32.6,
                     seeds: 66, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGQAWGFDRHGGPEVQAFFDRPDPVPGRGEVLIRVDVAGVNPLDH
                     LLRSGPVDGLDGGRPFPRVLGMEAAGTVLARGQDVTGLEVGDAVFGFALTGGGTYAQTT
                     VLSAANTARIPAGLSATVAATLPVAGTTAVDVLDQLGLPAGATVLVNGVGGGVGLAVTR
                     LAIGRRLHVIGTGSTAKREHAESLGALFIDYTTQDVAAAARKLVPDGFDAIVDLVAGTS
                     LRTIAPLARDPRNIIAVSDTSVPDLGGRFVERRLDRKNLERSARLALDGLLTPAITAVH
                     PLSEAPAALAAVENGHTTGKTVIKVT"
     aSDomain        complement(1978..2478)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B6R96_RS35895_PKS_ER.1"
                     /evalue="1.70E-29"
                     /label="B6R96_RS35895_PKS_ER.1"
                     /locus_tag="B6R96_RS35895"
                     /protein_end="189"
                     /protein_start="22"
                     /score="94.9"
                     /tool="antismash"
                     /translation="DPVPGRGEVLIRVDVAGVNPLDHLLRSGPVDGLDGGRPFPRVLGM
                     EAAGTVLARGQDVTGLEVGDAVFGFALTGGGTYAQTTVLSAANTARIPAGLSATVAATL
                     PVAGTTAVDVLDQLGLPAGATVLVNGVGGGVGLAVTRLAIGRRLHVIGTGSTAKREHAE
                     SLGA"
     CDS_motif       complement(2050..2112)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B6R96_RS35895_0001"
                     /evalue="1.90E-03"
                     /label="PKSI-ER_m5"
                     /locus_tag="B6R96_RS35895"
                     /protein_end="165"
                     /protein_start="144"
                     /score="11.0"
                     /tool="antismash"
                     /translation="GATVLVNGVGGGVGLAVTRLA"
     misc_feature    complement(2632..2647)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.24"
                     /tool="antismash"
     gene            complement(2643..3047)
                     /locus_tag="B6R96_RS35900"
                     /old_locus_tag="B6R96_35900"
     CDS             complement(2643..3047)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1171:
                     transcriptional regulator, MerR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018552569.1"
                     /locus_tag="B6R96_RS35900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_35900"
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_081521179.1"
                     /transl_table=11
                     /translation="MRIGELARRTGVSPRSLRYYEDQGLLTSSRSDAGQRHYADAAVQR
                     VALIRQLFDAGLSSRVIATVLPCVDIPGDPGVAEETFTAMMRERDRIDADIAHLIETRD
                     ALDALIRANSRHRAQLSTAPTTGGAADLML"
     misc_feature    4366..4380
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.8"
                     /tool="antismash"
     gene            complement(5001..5960)
                     /locus_tag="B6R96_RS35905"
                     /old_locus_tag="B6R96_35905"
     CDS             complement(5001..5960)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106284.1"
                     /locus_tag="B6R96_RS35905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_35905"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_237291262.1"
                     /sec_met_domain="AfsA (E-value: 1.2e-20, bitscore: 72.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTMLTIGKHAPVLPLPGTEQPWMRCNDARQLTTTVPREYVHRSSL
                     AEVFLTGCERTGQDTFTLTGQWPRAHTFFTSADGTGHDPVQAGETVRQVGLYLCHAVYG
                     VPLGHNVLLWSLDFTTRPEHLRIGRGPSDLTMNALCTDFGWKGNRFSGYLSVDIHRNGE
                     LAARGTARFSCVAPATYRRLRGDRGRLALTRPLPRRTPVPAHTTGRLLPFDVVLAPTDH
                     PGRWQLNPDLDHPILFEHANDHHPGMVLVEAARQAASALHAPATFTPAAIATEFHHYAE
                     LDAPCWIDATLTAPGHVAITGHQENRTIFRSTVTATTT"
     misc_feature    complement(5969..5984)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.07"
                     /tool="antismash"
     misc_feature    complement(6298..6313)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.25"
                     /tool="antismash"
     gene            6449..7807
                     /locus_tag="B6R96_RS35910"
                     /old_locus_tag="B6R96_35910"
     CDS             6449..7807
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385836.1"
                     /locus_tag="B6R96_RS35910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_35910"
                     /product="cytochrome P450"
                     /protein_id="WP_081521178.1"
                     /sec_met_domain="p450 (E-value: 1.2e-13, bitscore: 49.3,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRQCPFAIDALGGDVHAEAARVREQGPATRVLLPGGVSAWAVGR
                     AADMRRLLVDPRVSKDAYRHWPAWISGEVGEQWPLAIWVSVQNMITAYGSEHARLRRPA
                     AAAFTARRAAGLRPRIEEVVGELLEDLAASAPGGAVDLRTRFAHELPTRIVFELFGIPE
                     HSWGPLHKIINAFFDTGITAEEAQRNAADLDVVMADLVAHRRRHPGDDMTSALIAARDT
                     ATDPRTATDPRTATDARTATDARTATATAADAGAGAGAEGAGLSEKELVDSLILLFTAG
                     YETSVNLICTALHELLRNPAQLALVRAGLASWEDVVEEALRIEPPAFYGLLRFAVEDID
                     MGEGVVIGQGDPILISFGSAGRDPEVHGADADRFDVLRATRGQHLSFGFGTHHCLGATL
                     ARLECAVAVRRFFETFPAAALAGAGELGRVESFISNGLRELPVVLGPRAPVAD"
     gene            7928..8608
                     /locus_tag="B6R96_RS35915"
                     /old_locus_tag="B6R96_35915"
     CDS             7928..8608
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676872.1"
                     /locus_tag="B6R96_RS35915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6R96_35915"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_030390789.1"
                     /transl_table=11
                     /translation="MARQERAVRTRRAILEAAAAVFDERGYDAATIADILARAGVTKGA
                     LYFHFSSKQELAQGVLDEQFAEGGVPHRESKLQELVDVAMVLAYRMKREPMLSAGARLS
                     LGPGMRDIFGGGSVPGWIEFTRTLLVRAKEQGELLPHVDPAETAWILSACWTGAQIYSQ
                     TLSDRSDIEQRVAAVYQHICPSIATPAMLARLDIAGDRGERVSAEMRRGPGRGAAQESE
                     PALS"
     gene            8782..9849
                     /locus_tag="B6R96_RS38875"
     CDS             8782..9849
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="B6R96_RS38875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_162498483.1"
                     /transl_table=11
                     /translation="MVKQERAARTREALVRAAAEVFAEEGFTAASIAVISRRARVSAGG
                     LHFHFAGKTVLARAVQDRAAQALSRITAGPAVPPTAPASAPTTPGPGPTTARTTTARTT
                     TARTTTARTTTARTTPAPGPASAPGPAPASAPTAPASAPGPAPGPAQGPAPASAPGSAP
                     ASAPGPASAPSAPAPGPASAATTPAPVPGPDGSAGTGADPGGNAGGSAGAGSGGGSLQV
                     LVDSTHRLMALLAHDAVVRAGFALCADPSFDGTADLRRQWRLWVEAAVAAAAREGLLAE
                     GLLPEDAARGVVAATVGLEVLGAYDRQWLAPHTLTRFWTLMLPRLASPPALDALLPQGS
                     PGSAAPPPDPDTESD"
     misc_feature    complement(10017..10031)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.18"
                     /tool="antismash"
     misc_feature    10048..10063
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.92"
                     /tool="antismash"
ORIGIN
        1 ccggcgttgt tgaaggcggt gtcgaggtgg ccgtaggtgg tgatggcttg ttggacggcg
       61 tgggcgattt gttcggtgtg ggtgacgtcg gcgatgacgt attgggcgtg gtggccttgg
      121 gtttgtagtt cgtcggtgag gtcggcgagt tgtttttcgc ggcgggcgac gagggtgacg
      181 gcggcgcctt cgcggcagaa gatgcgggcg gcggcggctc cgatgccgga gcttgcgccg
      241 gtgatgagtg cggatctgcc ttcgaggagc ttgggttggg ttccggcggt catcgcgtga
      301 cggtaacacg gtggattggg gggttgtggt gttcggtggg ggtgggttgg tgttggtgac
      361 ggggggtgcg gggtttgtgg gttctgtggt ggtggagagg ttggtggggt ggggtgttgg
      421 ggtgagggtg ttggtgcatc gggcgggggt gtcgcgggtg ggggtggagg tggtgcgggg
      481 ggatttggtg cggccgggtt cgttgcgtgg ggtgtgtgag ggggtgtcgt cggtgttgca
      541 tttggcgtcg cggatcgggg ggtcggtggg ggagttggag gcggtgaatg tggaggggac
      601 gcgggcgttg ttgggggagg cggcgcgggc gggggtggag cgggtggtgc agttggggac
      661 ggcgggggtg tatggggatg gggtgcatcg ggggtggtgg agggggagtt ggtggagtgt
      721 ccggggtcgg tgacgagtgt gacgcggttg gcgggggagc ggttggtgtt ggcggcgggt
      781 gggacggtgg tgcggccgca tttggttttc gggcgggggg atcggtgggt ggtgccgggg
      841 ttggtggagt tgttggggcg tgtgccggtg tgggtggagg gtgggcgggc gcgtttgtcg
      901 ttggtttcgg tggatgtgtt ggcgggggtg ttggcggagt tggcggtggg gggtgggcgg
      961 gtggggggtg gggtgttgca tgcggggcat ccggagccgg tgagtgtgcg ggtgttggtg
     1021 gagacggttg cgggtgcgtt ggggttgggg ttgccggtgg gggaggttga tctggcgggg
     1081 gcgttggagt gggtgggggg tgcgcagggt ggggtgttgg cgcggcgggt gcggttggtg
     1141 ggcgttgatc gctggtatga cagtgggcgg ttgtggtcgc aggtgagggt ttcgccgggg
     1201 cctgggttcg tggaggcgtt cgcggagtgt gctccgtggt accggtcggt gctgtcgggc
     1261 gggaaccctg cctgacggcc ccgatgctct ccccctcctg ggccggccca tgcgccctgg
     1321 gcgccgccac gggtttggca gggcgtgaac atcgtgtgtc acgcagtgtg ttgtccgccg
     1381 gagagccggg ctgggtcttc cttgtgtccg gtgtcttggg ccccgggtgg tggggtggtg
     1441 gcttggtgtg ggcgcgggct ggtggcgtac cggtgaaggg gttcgactcg gtacaggtga
     1501 atgtgctcgg tcgatggtgc gccgaccgtg ggtcacgttc gtcccggctc tgcctcggga
     1561 ggttgtcgtt cttggcggtc cgccttggcc ggttgacgtt gtcgggtggc cgggtgtttc
     1621 ttatgtcacc ttgatgacgg tcttgccggt ggtgtggccg ttctcgacgg cggcgagggc
     1681 ggccggggct tcggagagcg gatggactgc ggtgattgcg ggtgtgagga gtccgtcgag
     1741 ggcgagtcgg gctgatcgct ccaggttctt gcggtcgagg cggcgctcga cgaagcgccc
     1801 gccgagatcg ggcacggatg tgtcgctgac ggcaatgatg ttgcgggggt cgcgggccag
     1861 gggagcgatg gttcgcagtg aggtgcctgc gaccaggtcg acgatcgcgt cgaagccgtc
     1921 cggaacgagc ttgcgtgccg cggcggcgac gtcctgggtg gtgtagtcga tgaagagcgc
     1981 ccccaggctc tcggcgtgtt cgcgtttggc ggtgctcccg gtgccgatca cgtgcagccg
     2041 gcgtccgatg gccagccggg tgacggcgag gccgactccg cctccgactc cgttgaccag
     2101 gaccgtggcg ccggcgggga gtccgagttg gtcgaggacg tccaccgcgg tcgtcccggc
     2161 caccggcagt gttgccgcca cggttgcgga caggcccgcc gggatgcgcg cggtgttcgc
     2221 cgcggacagc accgtggtct gggcgtaggt gccgccaccg gtcagcgcga agccgaagac
     2281 tgcgtcgccc acctcgagcc cggtgacgtc ctggccccgg gcgagaacgg ttccggctgc
     2341 ctccatgccc agcacgcggg gaaagggacg cccgccgtcg agcccgtcga ccgggcccga
     2401 gcgcagcagg tggtcgaggg gattgacgcc ggcgacgtcg acccggatca gtacctcacc
     2461 gcgaccgggt acgggatcgg ggcgatcgaa gaacgcctgc acctcgggcc cgccgtgcct
     2521 gtcgaaaccc catgcctgtc ccatcttccg ccgcctctcg tacgaccggc ccgctgaatg
     2581 cgggcgctgt cgccaccctc acccctgcca ctgatgtcag gggcaacacg atgagatgca
     2641 ggtcacagca tcaggtcggc tgcgccgccg gttgtcgggg ccgtggacag ctgcgcccgg
     2701 tgccggctgt tggccctgat cagtgcgtcg agtgcatccc gggtctcgat caggtgcgcg
     2761 atatcggcgt cgatccggtc gcgctcgcgc atcatcgccg tgaacgtctc ctcggcgacg
     2821 cccgggtcac cggggatgtc cacacacggc agcacggtcg cgatcacccg gctggacagc
     2881 cccgcgtcga acaactgcct gatgagcgcc acgcgctgga ccgcagcatc ggcgtagtga
     2941 cgctgccccg cgtcggagcg tgagctggtc agcagcccct ggtcctcgta gtaccgcagt
     3001 gagcgcgggc tcacgcccgt gcgccgggca agctcgccta tccgcatccc gcagagccta
     3061 cgcccgcgac ctgcgggtgc ggagcgccga tgcgtcatgg tcgcctgcgg acacccccta
     3121 ccgcggcagg gttgccgtgc ccgccgcccc acggctgagc acgttcgccc gtacacggct
     3181 gagcccttgt cgccgtatgc cggcacgggc tgccgccagg gggctcggtg cggcgcccgg
     3241 ggacgggggc acgcccggct tcggcgcccc gcggcccatg acgccgccac atcccactcg
     3301 gtgggcgtga acttctgctg ccgccggggg tgttgccagg ccgggggcag ccgggtcggg
     3361 tcgtccttgt gtccggtgtc tgcgtcccct ggttccgggg cggggggcct ggtatggatg
     3421 cgggctgccg tcagggggtg gccggtcgga ggtcctgggc cgggtgaccc tgctgtccgg
     3481 ggaccggtgg cccgggtccc gggtcctggg tcgtggccgt gcgtgaacac cggatgggcg
     3541 cgggtgctgt cgggtcggtc gcccgggtcg ggcggcccct ggtttcgtgc ctgaactttc
     3601 gtgccgggtt gcggccccgg cgtggacatc ggctgccgca ggggcgtgtc tgtcccgatg
     3661 cccggggcgg gatcccgtcc cgtgcccgca cgcccctggt ttaccggcag ggcctgggcg
     3721 tgcgcatcgt gcgcttgggc gggtgtggtg cggtcggtgt ccgggccggg tcgcccctgc
     3781 gttccgttgc gtgccgttgc gtttcgttgc ctgcgcccct ccgggccctc gttacgggcc
     3841 ctggcgtgaa cggggctgcc gtgcggggct gtcgggctgc tcgcccggca ccggacaccc
     3901 ccgtttcggt gtggtgcagg tgcgcagggc ggcggctccg gcatgaacat gggcggtggt
     3961 gggggaggtt gtcggggcgg ccgtccgcgg atgagcctcc tccgcttcgg ggactgcgcc
     4021 cccacgtgcc gtgccgtgcc gtgccggggc gttggtgtgg actgcggttg ctgcaggggg
     4081 tccgaccgat cccgtccggg cggccgcacc gtgttcggcc gctgcgtgcc gggtttcccg
     4141 ggcggcggct ggggtgcggg gccgctccga ggtcccggcc ggacactctg gtgcggcgtg
     4201 cctgcccgcg ccgtggtgcg gggcgatggt cctggcgtga accacgggct gctgcggggg
     4261 cccggcgtgg acagcggctg atgcgagggg gccggcacga cgccccggac cggagccccg
     4321 gctgcgctgg gcgcgctctt ggcgccgtca tggctccggc tggcgtgaac gtcgttgtcg
     4381 gtaaagggtg ctgtccccgg gcagccgatg cgagtccatc ccggtgcccc cggtgttcgc
     4441 gccccctggt tcagggacgg gggcccggtg tggacgcggg ctgctgcggg ggaggtgtcc
     4501 ggtcggttgc ccgggtcggc tccccccttt gttgggcgtg ctgcgccccg tggtgggggg
     4561 cgttcggtgc cgctgggagt ggtccccgcc ggtgttccgg ccgggggttc ctgcggcgcc
     4621 gactgggccg gtggtgtgcg ggggcatggg gcgggacccc cggtgtgaac ggtgtcgcgt
     4681 ggtggggggt gttgtccggt cgggggtggc gaggccgttg ccgggggagg tgtttcccct
     4741 tttgtgcggg cgggtgtgcg cctgcctgtc cgcccccggc ccccggtgtg aacggtgttg
     4801 cgtgctgggg gtgttgcccg gtcgcggtgg tgaagccgcc ctccggggaa ggtgtgcccg
     4861 ctcttgtgcg ggcggggtgt gtgcctgtcg gctcgccccg ccccggctcc cgatgtccgc
     4921 gctgcccccg ccgttcccgc ggcacccccg accccgacgc ccgcggcccg accccgacgt
     4981 cggacgccgg acagcggtgg tcaggtggtg gttgcggtga cggtggagcg gaagatggtg
     5041 cggttttcct ggtggccggt gatggcgacg tggccggggg cggtgagggt ggcgtcgatc
     5101 cagcaggggg cgtcgagttc ggcgtagtgg tggaattcgg tggcgatggc ggcgggcgtg
     5161 aaggtggcgg gggcgtggag ggcgctggcg gcctggcggg cggcttcgac gaggaccatg
     5221 ccggggtggt ggtcgttggc gtgttcgaag aggatggggt ggtcgaggtc ggggttgagt
     5281 tgccagcggc cggggtggtc ggtgggggcg aggacgacgt cgaagggcag gagccggccg
     5341 gtggtgtggg cggggacggg ggtgcggcgg ggcagggggc gggtcagggc caggcggccg
     5401 cggtcgccgc gcaggcggcg gtaggtggcg ggggcgacgc aggagaagcg ggcggtgccg
     5461 cgggcggcga gttcgccgtt gcggtggatg tcgacgctga ggtagccgga gaaccggttg
     5521 cccttccagc cgaagtcggt gcacagggcg ttcatggtga ggtcgctggg gccgcggccg
     5581 atgcgcaggt gttcggggcg ggtggtgaag tccaggctcc acagcaggac gttgtggccg
     5641 agggggactc cgtagacggc gtggcagagg tagaggccga cctggcggac ggtctcgccg
     5701 gcctggacgg ggtcgtggcc ggtgccgtcg gcgctggtga agaaggtgtg ggcgcggggc
     5761 cactgcccgg tcagggtgaa ggtgtcctgg ccggtgcgtt cgcagccggt caggaagacc
     5821 tcggccagtg agctgcggtg gacgtattcc cggggcacgg tggtggtcag ctggcgggcg
     5881 tcgttgcagc gcatccatgg ctgctccgtc ccgggcaggg gcaggacggg cgcgtgcttt
     5941 ccgatcgtca gcatggtcat gacttctccc gcagagccgt tcgacggatg ggacgggttg
     6001 cgctcgcgag acgcaagata cgtactctcc ggttttgttt gcaaggagct cagccgggca
     6061 ggatgcggcc cgcacgtcgc cccggcggcc cgccgggcgc cctccgggag ggctgtcagg
     6121 acctggacca gcggatatcc gacagggtgc ggccgcaggg gacgcgcaca ccaccgcccc
     6181 aagtgggaac cgtggttccg gtcatatgcg ccggtcggac agggtgcggg cgccgtccgt
     6241 tcaaccaggc ccccgccagg gcgacttcgg gggaccggat gcggcgggcc gggtcccgaa
     6301 aaccattggc atgcgggtcg ggtcgtgtca gactcggggc cgccgccggt gaaacagaaa
     6361 ccggatgggc ggttttggaa tacggggtgt ggacacgccg ctgcaccctg tcgccgatct
     6421 gccgccgtcc atcctggaga gttgtgagat gaaccgtcag tgcccttttg cgatcgacgc
     6481 cctgggcggg gacgtgcacg ccgaggccgc gcgcgtgcgt gaacaggggc ccgcgacacg
     6541 ggtcctgctg ccgggcgggg tcagtgcctg ggccgtgggc cgcgcggccg acatgcgccg
     6601 gctcctggtg gacccgcggg tctccaagga cgcctaccgc cactggccgg cctggatcag
     6661 cggcgaggtg ggggagcagt ggccgctggc gatctgggtc tcggtgcaga acatgatcac
     6721 cgcctacggc agcgagcacg cccggctgcg cagaccggcg gcggccgcgt tcaccgcccg
     6781 acgggcggcc ggcctgcgcc cccggatcga ggaggtggtc ggtgaactcc tggaggacct
     6841 ggccgcctcg gcgcccggtg gcgcggtgga cctgcgcacg cggttcgccc acgagctgcc
     6901 gacccggatc gtcttcgagc tgttcgggat accggagcac tcctggggcc cgctgcacaa
     6961 aatcatcaac gcgttcttcg acaccggtat caccgccgag gaggcgcagc gcaacgcggc
     7021 cgatctggac gtggtgatgg cggacctggt cgcccaccgc cgccgccacc ccggcgacga
     7081 catgaccagc gcactcatcg ccgcccgcga caccgccacc gacccccgca ccgccaccga
     7141 cccccgcacc gccaccgacg cccgtaccgc caccgacgcc cgtaccgcta ccgctaccgc
     7201 cgccgatgcg ggtgcgggtg cgggtgcgga gggggcgggg ttgagcgaga aggagctcgt
     7261 cgacagcctg atcctgctct tcacggccgg ttacgagacc tccgtcaacc tgatctgcac
     7321 ggccctgcac gaactgctgc gcaatccggc gcagttggcg ctggtgcggg cggggttggc
     7381 ttcgtgggag gacgtggtgg aggaggcgct gcggatcgag ccgccggcct tctacgggct
     7441 gctgcgtttc gcggtggagg acatcgacat gggggagggg gtggtgatcg ggcagggtga
     7501 tccgatcctg atctccttcg gttcggcggg gcgtgatccc gaggtgcacg gcgcggacgc
     7561 cgaccgcttc gacgtgctgc gggcgacgcg ggggcagcat ctgtcgttcg ggttcggcac
     7621 gcatcactgc ctgggtgcga cgctggcgcg gctggagtgc gcggtggcgg tgcggcggtt
     7681 cttcgagacg ttcccggcgg cggctctggc gggggcgggg gagttggggc gggtggagtc
     7741 gttcatctcc aacgggctgc gggagttgcc cgtggtgctg ggtcccaggg cgcccgtggc
     7801 cgattgaccg cgtcggggcg acgcctggcc tgcttgaaaa gaatcggacc ttctggttgg
     7861 ttttaatggg gcggtggatc cgcgcggtgg atccggccgt attggaccat ggacaacgga
     7921 ggcaccggtg gcacggcagg agcgtgcggt acgtacgcgt cgcgcgatct tggaagcggc
     7981 ggcggcggtc ttcgacgagc gcggttacga cgcggccacc atcgcggaca tcctcgcgcg
     8041 ggcgggcgtc accaagggcg ccctgtattt ccatttctcc tccaagcagg agctggcgca
     8101 gggcgtcctg gacgagcagt tcgccgaggg cggggtgccc caccgggaga gcaagctgca
     8161 ggaactggtg gacgtcgcga tggtgctggc gtaccggatg aagcgcgagc ccatgctcag
     8221 cgcgggtgcc cggctctctc tggggccggg catgcgcgac atcttcggcg ggggttcggt
     8281 gccgggctgg atcgagttca cgcggaccct gctggtgcgg gcgaaggagc agggtgagct
     8341 gctgccgcac gtggatcccg ccgagacggc ctggatcctg tccgcctgct ggacgggcgc
     8401 gcagatctac tcgcagaccc tgagcgaccg cagcgacatc gagcagcggg tggccgccgt
     8461 ctaccagcac atctgcccga gcatcgcgac gcccgcgatg ctcgccaggc tcgacatcgc
     8521 cggcgaccgg ggggagcggg tgagcgcgga gatgcggcgc gggcccggcc ggggggcggc
     8581 gcaggagagc gaaccggccc tgtcctgagg cccgcccgcc ccctgcccgc ccgagggcgg
     8641 cccccggtgg ggcgggtggg gccggtgggg ccggccggct ccagcgggac gcgagcgggg
     8701 ctccggtggc ggcgcggacc gtgaccggaa cgatctttac acccccttgg gcaaaccgcg
     8761 ttggcggttt gtggagactc catggtcaag caggaacgtg cggcccgtac gcgggaggcg
     8821 ctggtgcgtg cggccgcgga ggtgttcgcc gaggaggggt tcaccgccgc gtcgatcgcg
     8881 gtgatcagcc gccgggcccg ggtcagcgcc gggggactgc acttccactt cgccggcaag
     8941 accgtcctgg cccgcgccgt ccaggaccgg gccgcgcagg ccctgtcgcg catcacggcc
     9001 ggcccggccg taccaccgac cgcccccgcc tccgccccga ccacccccgg accgggtccg
     9061 actaccgccc ggaccaccac cgcccggacc accaccgccc ggaccaccac cgcccggacc
     9121 accaccgccc ggaccacccc cgcgccgggt ccggcctccg cgccgggtcc ggctcccgca
     9181 tccgcgccga ccgcccccgc ctccgccccg ggccccgccc cgggccccgc ccagggcccc
     9241 gcccccgcct ccgccccggg ttccgccccc gcctccgccc cgggccccgc ctccgccccg
     9301 tccgcccccg caccgggccc tgcctccgca gcgaccaccc cggcccccgt cccgggcccg
     9361 gacgggagcg cgggcaccgg cgcagacccg ggcgggaacg caggcgggag tgcgggcgcc
     9421 ggttcgggcg ggggttcgtt gcaggtgctg gtggactcca cgcaccggct gatggccctc
     9481 cttgcgcacg acgcggtggt gcgggccggg ttcgcgctgt gcgcggaccc ctccttcgac
     9541 gggacggcgg acctgcgccg gcagtggcgg ctgtgggtcg aggcggccgt ggcggccgcg
     9601 gcccgggagg gcctgctcgc cgaggggctc ctgcccgagg acgcggcgcg cggggtggtg
     9661 gccgcgacgg tggggctgga ggtcctgggc gcctacgacc ggcagtggct cgcaccgcac
     9721 accctgaccc ggttctggac gctgatgctg ccgcggctcg cctcgccccc ggccctggac
     9781 gccctgctgc cgcagggatc gcccggcagt gccgccccac cccccgaccc ggacaccgaa
     9841 tcagactgaa cggtctcttt caggtggggt ccgccaccct cctgtgaccc tcgtcgaggg
     9901 ggtgatcgag gcgtcctgaa gccgccccac cgcacccgaa cccacccgaa attccaggcc
     9961 agcaaaggtt tctgacgttc cgtcggacat gatcgaccat caaaacagca cgaccggttt
    10021 gatattgaca aaccgccggt cctgttttct actggctgtg cgactcatcc aaggacaggg
    10081 gagtacggcg tggacatcga tgtactgggc gcgttggcgg tgcgggagaa cggaatctcc
    10141 atcacgccga ccgcccccaa gccccggcag gtcctggccc tcctggccct ccatgccgat
    10201 caggtggtac ccgtatcggc actgatagag gaactgtggg cgggcgaacc gccgcgcagt
    10261 gcccgcacca cactgcagac ctacgtcctg cagttacgcg ccctcatcgc cggcgccctc
    10321 gaacgcggca ccgccaccgc caccggcccc gacgccgggg ccgacgacga cacccccacc
    10381 ggtacgggtg cgggtgcggg tgcggggcgg gtggtgcgga ccgccaagga catcctggtc
    10441 accctgcccg gcggatacct cctcagcagc ggcggcggaa ccctcgacgt ccgcgagttc
    10501 gaccgcctcg ccggcaccgg ctaccgcgcc atggacgccg gcgacttccc cggcgccgcc
    10561 cgcctgctgc gcgaggccct ggccctgtgg tccggacccg ccttcgccga cgtacagggc
    10621 ggaatccagc tcgacatgga gaccagacgc ctggaagaga cccggctgtg cgccctggac
    10681 cagcggatcg aggccgacct gcgactggga cgccaccgcg aactcctggc cgaactgacc
    10741 gtcctggtca cccgctaccg cacccacgag aacctccacg gacagttcat gctcgccctg
    10801 caccgctccg gccgccgcgg cgaggcactg gacgtctacc agcgactgcg cgccaccctc
    10861 gtgcgcgaac tgggactgga accctccatc gccctgcgcc gcctgcagcg ctcgatcctg
    10921 atggccggac ccgagagccc ggccgaggcc cacgcggagc
//
